CN207452141U - A kind of control microcarrier cell is inoculated with adherent uniformity device - Google Patents
A kind of control microcarrier cell is inoculated with adherent uniformity device Download PDFInfo
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- CN207452141U CN207452141U CN201721541278.5U CN201721541278U CN207452141U CN 207452141 U CN207452141 U CN 207452141U CN 201721541278 U CN201721541278 U CN 201721541278U CN 207452141 U CN207452141 U CN 207452141U
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Abstract
The utility model discloses a kind of control microcarrier cells to be inoculated with adherent uniformity device, including insulating layer, ultraviolet sterilizing lamp, blake bottle sealing cover, filtering sandwich layer, sensing switch board and jet through hole, the insulating layer is internally provided with ultraviolet sterilizing lamp, filtering sandwich layer is provided with below the blake bottle sealing cover, jet through hole is offered on the outside of the sensing switch board, the utility model is scientific and reasonable for structure, safe and convenient to use, the temperature needed during cell culture can be provided by being provided with insulating layer；Being provided with ultraviolet sterilizing lamp can go out except the thalline of cell interior doping, improve the health of cell passage；It is provided with blake bottle sealing cover and improves air-tightness inside blake bottle；Leucocyte and filtering foreign can be filtered out by being provided with filtering sandwich layer；Cell quality, pH value, the quantity of electric charge and physiological environment temperature etc. can be detected by being provided with induction controller；Being provided with jet through hole can make its adherent more uniformly in cell attachment incubation.
Description
Technical field
The utility model is related to cell culture equipment technical fields, are specially that a kind of control microcarrier cell inoculation is adherent
Evenness device.
Background technology
Microcarrier cell inoculated and cultured technology, is mainly used for the large-scale culture of zooblast, by stirring in incubation
Mixing makes cell suspend and adherent, carries out culture inoculation, since zooblast quality itself and its charged property are not phases
With, lead to problems such as its often occur during adherent it is adherent it is uneven, accumulation is serious, space between cells is excessive, reduce
The quality and the survival rate of cell that cell inoculation is cultivated, while zooblast inner structure is complicated, would generally be mixed before cell culture
It is miscellaneous to have leucocyte and other mushroom sundries, cause it has impact on the normal of cell to cultivate and be inoculated with, and to the life of cell culture
Reason environment pollutes, while cell, in seeded process is cultivated, the internal temperature of incubator determines that it cultivates the speed of inoculation
Degree and survival rate, so insulating layer and heater are essential for cell culture, the air-tightness inside incubator and blake bottle
The joint factor of cell culture inoculation is also become, the stability of incubator installation, which makes cell possess in seeded process is cultivated, to be had
Certain guarantee.
The content of the invention
The utility model provides a kind of control microcarrier cell and is inoculated with adherent uniformity device, can effectively solve the above-mentioned back of the body
Quality of the zooblast proposed in scape technology itself and its charged property are different, cause it during adherent
Often there is the problems such as adherent uneven, accumulation is serious, space between cells is excessive, reduce the quality and cell of cell inoculation cultivation
Survival rate, would generally be affected the normal of cell doped with leucocyte and other mushroom sundries and cultivate and connect before cell culture
Kind, insulating layer and heater are essential for cell culture, and the air-tightness inside incubator and blake bottle also becomes cell
The problems such as cultivating the joint factor of inoculation.
To achieve the above object, the utility model provides following technical solution：A kind of control microcarrier cell inoculation is adherent
Uniformity device, including device body, insulating layer, external-connected port, stationary fixture, spill fixing groove, micro-carriers cell culture case,
Micro-carriers cell culture bottle, blake bottle side wall, ultraviolet sterilizing lamp, blake bottle sealing cover, filtering sandwich layer, shaft, sensing control
Cabinet, paddle, micro-driving motor, fixed strut, pH value buffer solution adding mouth, culture solution adding mouth, control switch, power supply,
Snorkel, movable loop bar, sealant rubber ring, vibrator, gas collection chamber, jet through hole, miniature gas collection air pump, heater, PLC controls
Device, slide, charge density detector, charge mass detector, pH value detector, temperature monitor and connect partition plate, the dress
It puts and insulating layer is provided on the inside of body, described device body bottom portion both sides are both provided with stationary fixture, the stationary fixture top
Slide is provided with, incubator is provided with above described device body inside bottom, it is thin to be provided with microcarrier on the inside of the incubator
Born of the same parents' blake bottle, the micro-carriers cell culture bottle edge both sides are both provided with blake bottle side wall, described device body interior or so
Both sides are both provided with ultraviolet sterilizing lamp, and the micro-carriers cell culture bottle upper center is provided with blake bottle sealing cover, described
It is provided with filtering sandwich layer below blake bottle sealing cover, the micro-carriers cell culture bottle middle inside is provided with shaft, described turn
Sensing switch board is provided with below axis, the sensing switch board includes charge density detector, charge mass detector, pH value inspection
It surveys instrument, temperature monitor and connects partition plate, charge mass monitor, the charge are provided on the right side of the charge density detector
PH value detector is provided with below density tester, temperature monitor, the sensing control are provided on the right side of the pH value detector
Paddle is provided with below cabinet processed, top is provided with micro-driving motor on the right side of the incubator, and the micro-driving motor is left
Side-lower offers pH value buffer solution adding mouth, and culture solution adding mouth is offered on the left of the pH value buffer solution adding mouth, described
Control switch is provided on the right side of micro-driving motor, power supply, the shaft or so two is provided on the left of the micro-driving motor
Side is both provided with snorkel, and movable loop bar is provided on the outside of the snorkel, sealant rubber ring is provided with above the activity loop bar,
The snorkel lower right-hand side is provided with vibrator, and gas collection chamber, the gas collection chamber left side are provided on the left of the vibrator
Jet through hole is offered, lower section is provided with miniature gas collection air pump, the miniature gas collection air pump bottom right side on the left of the snorkel
Having heaters is set, and the miniature gas collection air pump left bottom is provided with PLC controller, the input terminal and electricity of the control switch
The output terminal in source is electrically connected, the micro-driving motor, ultraviolet sterilizing lamp and sense the input terminal of switch board with control
The output terminal of switch is electrically connected, and the output terminal of the input terminal and sensing switch board of the PLC controller is electrically connected, described micro-
The input terminal of type gas collection air pump and the output terminal of PLC controller are electrically connected.
Preferably, lower section offers external-connected port on the left of described device body.
Preferably, it is provided with spill fixing groove above described device body inside bottom.
Preferably, it is provided with fixed strut below the micro-driving motor.
Preferably, it is flexibly connected between the snorkel and miniature gas collection air pump by movable loop bar.
Preferably, connected between the micro-driving motor and paddle by shaft.
Preferably, between the charge density detector, charge mass detector, pH value detector and temperature monitor
It is connected by connecting partition plate.
Compared with prior art, the beneficial effects of the utility model：The utility model is scientific and reasonable for structure, side safe to use
Just, the temperature needed during cell culture can be provided by being provided with insulating layer；Being provided with ultraviolet sterilizing lamp can go out except thin
The thalline of intracellular portion doping improves the health of cell passage；Be provided with blake bottle sealing cover improve it is airtight inside blake bottle
Property；Leucocyte and filtering foreign can be filtered out by being provided with filtering sandwich layer；Be provided with induction controller can detect cell quality,
PH value, the quantity of electric charge and physiological environment temperature etc.；Being provided with jet through hole can make its adherent more in cell attachment incubation
Add uniformly.
Description of the drawings
Attached drawing is used to provide a further understanding of the present invention, and a part for constitution instruction, with this practicality
New embodiment for explaining the utility model, does not form the limitation to the utility model together.
In the accompanying drawings：
Fig. 1 is the structure diagram of the utility model；
Fig. 2 is the mounting structure schematic diagram of the utility model jet through hole；
Fig. 3 is the mounting structure schematic diagram of the utility model sensing switch board；
Fig. 4 is the mounting structure schematic diagram of the utility model stationary fixture；
Fig. 5 is the mounting structure schematic diagram of the utility model pH value buffer solution adding mouth；
Fig. 6 is the mounting structure schematic diagram of the utility model culture solution adding mouth；
Figure label：1st, device body；2nd, insulating layer；3rd, external-connected port；4th, stationary fixture；5th, spill fixing groove；6th, it is micro-
Carrier cell incubator；7th, micro-carriers cell culture bottle；8th, blake bottle side wall；9th, ultraviolet sterilizing lamp；10th, blake bottle seals
Lid；11st, sandwich layer is filtered；12nd, shaft；13rd, switch board is sensed；14th, paddle；15th, micro-driving motor；16th, fixed strut；
17th, pH value buffer solution adding mouth；18th, culture solution adding mouth；19th, control switch；20th, power supply；21st, snorkel；22nd, movable sleeve
Bar；23rd, sealant rubber ring；24th, vibrator；25th, gas collection chamber；26th, jet through hole；27th, miniature gas collection air pump；28th, heater；
29th, PLC controller；30th, slide；31st, charge density detector；32nd, charge mass detector；33rd, pH value detector；34th, it is warm
Spend detector；35th, partition plate is connected.
Specific embodiment
The preferred embodiment of the utility model is illustrated below in conjunction with attached drawing, it should be understood that described herein excellent
Embodiment is selected to be only used for describing and explaining the present invention, is not used to limit the utility model.
Embodiment：As shown in Figs. 1-5, the utility model provides a kind of technical solution, a kind of control microcarrier cell inoculation
Adherent uniformity device is thin including device body 1, insulating layer 2, external-connected port 3, stationary fixture 4, spill fixing groove 5, microcarrier
Born of the same parents' incubator 6, micro-carriers cell culture bottle 7, blake bottle side wall 8, ultraviolet sterilizing lamp 9, blake bottle sealing cover 10, filtering sandwich layer
11st, shaft 12, sensing switch board 13, paddle 14, micro-driving motor 15, fixed strut 16, pH value buffer solution adding mouth 17,
Culture solution adding mouth 18, control switch 19, power supply 20, snorkel 21, movable loop bar 22, sealant rubber ring 23, vibrator 24, gas collection
Chamber 25, jet through hole 26, miniature gas collection air pump 27, heater 28, PLC controller 29, slide 30, charge density detector
31st, charge mass detector 32, pH value detector 33, temperature monitor 34 are provided with partition plate 35,1 inside of device body is connected
Insulating layer 2,1 two bottom sides of device body are both provided with stationary fixture 4, and 4 top of stationary fixture is provided with slide 30, device body
Incubator 6 is provided with above 1 inside bottom, 6 inside of incubator is provided with micro-carriers cell culture bottle 7, micro-carriers cell culture
7 edge both sides of bottle are both provided with blake bottle side wall 8, and the 1 inside left and right sides of device body is both provided with ultraviolet sterilizing lamp 9, micro-
7 upper center of carrier cell blake bottle is provided with blake bottle sealing cover 10, and 10 lower section of blake bottle sealing cover is provided with filtering sandwich layer
11,7 middle inside of micro-carriers cell culture bottle is provided with shaft 12, and 12 lower section of shaft is provided with sensing switch board 13, sensing control
Cabinet 13 processed include charge density detector 31, charge mass detector 32, pH value detector 33, temperature monitor 34 with connect every
Plate 35,31 right side of charge density detector are provided with charge mass monitor 32, and 31 lower section of charge density detector is provided with pH
It is worth detector 33,33 right side of pH value detector is provided with temperature monitor 34, and 13 lower section of sensing switch board is provided with paddle 14,
6 right side top of incubator is provided with micro-driving motor 15, and 15 left side lower section of micro-driving motor offers pH value buffer solution and adds
Add mouth 17,17 left side of pH value buffer solution adding mouth offers culture solution adding mouth 18, and 15 right side of micro-driving motor is provided with control
System switch 19,15 left side of micro-driving motor are provided with power supply 20, and 12 left and right sides of shaft is both provided with snorkel 21, snorkel
21 outsides are provided with movable loop bar 22, and movable 22 top of loop bar is provided with sealant rubber ring 23, and 21 lower right-hand side of snorkel is provided with
Vibrator 24,24 left side of vibrator are provided with gas collection chamber 25, and 25 left side of gas collection chamber offers jet through hole 26, snorkel 21
Miniature gas collection air pump 27 is provided with below left side, miniature 27 bottom right side of gas collection air pump sets having heaters 28, miniature gas collection gas
It pumps 27 left bottoms and is provided with PLC controller 29, the input terminal of control switch 19 is electrically connected with the output terminal of power supply 20, miniature
Output terminal of the input terminal of driving motor 15, ultraviolet sterilizing lamp 9 and sensing switch board 13 with control switch 19 is electrically connected,
The input terminal of PLC controller 29 and the output terminal of sensing switch board 13 are electrically connected, the input terminal and PLC of miniature gas collection air pump 27
The output terminal of controller 29 is electrically connected.
For the ease of the connection of the control terminals such as device body 1 and external computer, in the present embodiment, it is preferred that device sheet
1 left side lower section of body offers external-connected port 3.
For the ease of the installation and fixation of incubator 6, in the present embodiment, it is preferred that set above 1 inside bottom of device body
It is equipped with spill fixing groove 5.
In order to be more fixed when running micro-driving motor 15, in the present embodiment, it is preferred that under micro-driving motor 15
Side is provided with fixed strut 16.
For the ease of adjusting the jet direction of jet through hole 26, in the present embodiment, it is preferred that snorkel 21 and miniature gas collection
It is flexibly connected between air pump 27 by movable loop bar 22.
In order to by stirring action microcarrier cell be enable to be suspended in culture solution, in the present embodiment, it is preferred that miniature
It is connected between driving motor 15 and paddle 14 by shaft 12.
In order to make charge density detector 31, charge mass detector 32, pH value detector 33 and temperature monitor 34 equal
Normal operation, and between do not generate reciprocal effect, in the present embodiment, it is preferred that charge density detector 31, charge mass detection
It is connected between instrument 32, pH value detector 33 and temperature monitor 34 by connecting partition plate 35.
The operation principle and process for using of the utility model：It first will by stationary fixture 4 and slide 30 by device body 1
It is fixed on work top, making not generate in the device course of work and rock, and it is micro- will to wait that the zooblast for cultivating inoculation is put into
In carrier cell blake bottle 7, filtering sandwich layer 11 can will be doped in intracellular leucocyte and some dirts during input
Thalline is filtered out in time, and blake bottle sealing cover 10 makes the air-tightness inside micro-carriers cell culture bottle 7 more preferable, can prevent the external world
Miscellaneous bacteria etc. is mixed into, and insulating layer 2 and heater 28 can provide cell required adaptation in cultivation, inoculation and succeeding generations
Temperature, ultraviolet sterilizing lamp 9 can eliminate the thalline inside micro-carriers cell culture case 6 and micro-carriers cell culture bottle 7, make thin
Born of the same parents gather around the physiological environment there are one more healthy cleaning, pass through micro-driving motor 15, shaft 1, paddle 14 and vibrator 24
The cell in micro-carriers cell culture bottle 7 can be made to be stirred and vibrate, it is made more fully to be suspended in culture solution, thin
When born of the same parents suspend, sensing switch board 13 can detect cell sole mass, charged amount, pH value, physiological environment temperature in culture solution
The data such as degree, can be by adding when cell charged amount and relatively low pH value in pH value buffer solution 17 casees culture solutions of adding mouth
Add appropriate amount of buffer solution, supplement the pH value concentration needed for cell culture, and inside equalization cell itself electrically charged amount polarity it is poor,
Then sense switch board 13 and detection data are transferred to PLC controller 29, PLC controller 29 is by cell sole mass and carries
The numerical values recited of the quantity of electric charge controls the spray of the work frequency of miniature gas collection air pump 27 and gas collection chamber 25 and jet through hole 26
Gas position, when cell sole mass and larger charged amount, gas collection chamber 25 and jet through hole 26 will be positioned at cultures
Bottle side wall 8 bottom position carries out jet, makes sole mass and the larger cell attachment of charged amount in the bottom of blake bottle side wall 8
Portion, when cell sole mass and smaller charged amount, gas collection chamber 25 and jet through hole 26 will be suspended into culture
For jet during bottle 8 top position of side wall, make its adherent in the top of blake bottle side wall 8, during jet is adherent, due to thin
The difference that born of the same parents carry weight and charged amount will not generate the phenomenon that accumulation and excessive adherent gap when making its adherent, last real
Now cell is uniform adherent.
Finally it should be noted that：The foregoing is merely the preferred embodiments of the utility model, are not limited to this reality
With new, although the utility model is described in detail with reference to the foregoing embodiments, come for those skilled in the art
Say, can still modify to the technical solution recorded in foregoing embodiments or to which part technical characteristic into
Row equivalent substitution.Within the spirit and principle of the utility model, any modifications, equivalent replacements and improvements are made, should all
It is included within the scope of protection of this utility model.
Claims (7)
1. a kind of control microcarrier cell is inoculated with adherent uniformity device, including device body（1）, insulating layer（2）, external-connected port
（3）, stationary fixture（4）, spill fixing groove（5）, micro-carriers cell culture case（6）, micro-carriers cell culture bottle（7）, blake bottle
Side wall（8）, ultraviolet sterilizing lamp（9）, blake bottle sealing cover（10）, filtering sandwich layer（11）, shaft（12）, sensing switch board
（13）, paddle（14）, micro-driving motor（15）, fixed strut（16）, pH value buffer solution adding mouth（17）, culture solution addition
Mouthful（18）, control switch（19）, power supply（20）, snorkel（21）, movable loop bar（22）, sealant rubber ring（23）, vibrator（24）、
Gas collection chamber（25）, jet through hole（26）, miniature gas collection air pump（27）, heater（28）, PLC controller（29）, slide（30）、
Charge density detector（31）, charge mass detector（32）, pH value detector（33）, temperature monitor（34）With connect partition plate
（35）, it is characterised in that：Described device body（1）Inside is provided with insulating layer（2）, described device body（1）Two bottom sides are equal
It is provided with stationary fixture（4）, the stationary fixture（4）Top is provided with slide（30）, described device body（1）In inside bottom
Side is provided with incubator（6）, the incubator（6）Inside is provided with micro-carriers cell culture bottle（7）, the microcarrier cell training
Support bottle（7）Edge both sides are both provided with blake bottle side wall（8）, described device body（1）The internal left and right sides is both provided with ultraviolet
Line sterilamp（9）, the micro-carriers cell culture bottle（7）Upper center is provided with blake bottle sealing cover（10）, the blake bottle
Sealing cover（10）Lower section is provided with filtering sandwich layer（11）, the micro-carriers cell culture bottle（7）Middle inside is provided with shaft
（12）, the shaft（12）Lower section is provided with sensing switch board（13）, the sensing switch board（13）It is detected including charge density
Instrument（31）, charge mass detector（32）, pH value detector（33）, temperature monitor（34）With connect partition plate（35）, the electricity
Lotus density tester（31）Right side is provided with charge mass detector（32）, the charge density detector（31）Lower section is provided with
PH value detector（33）, the pH value detector（33）Right side is provided with temperature monitor（34）, the sensing switch board（13）
Lower section is provided with paddle（14）, the incubator（6）Micro-driving motor is provided with above right side（15）, the micro drives
Motor（15）PH value buffer solution adding mouth is offered below left side（17）, the pH value buffer solution adding mouth（17）Left side offers
Culture solution adding mouth（18）, the micro-driving motor（15）Right side is provided with control switch（19）, the micro-driving motor
（15）Left side is provided with power supply（20）, the shaft（12）The left and right sides is both provided with snorkel（21）, the snorkel（21）
Outside is provided with movable loop bar（22）, the activity loop bar（22）Top is provided with sealant rubber ring（23）, the snorkel（21）
Lower right-hand side is provided with vibrator（24）, the vibrator（24）Left side is provided with gas collection chamber（25）, the gas collection chamber
（25）Left side offers jet through hole（26）, the snorkel（21）Miniature gas collection air pump is provided with below left side（27）, it is described
Miniature gas collection air pump（27）Bottom right side sets having heaters（28）, the miniature gas collection air pump（27）Left bottom is provided with
PLC controller（29）, the control switch（19）Input terminal and power supply（20）Output terminal be electrically connected, the micro drives
Motor（15）, ultraviolet sterilizing lamp（9）With sensing switch board（13）Input terminal with control switch（19）Output terminal it is electrical
Connection, the PLC controller（29）Input terminal with sensing switch board（13）Output terminal be electrically connected, the miniature gas collection gas
Pump（27）Input terminal and PLC controller（29）Output terminal be electrically connected.
2. a kind of control microcarrier cell according to claim 1 is inoculated with adherent uniformity device, it is characterised in that：It is described
Device body（1）External-connected port is offered below left side（3）.
3. a kind of control microcarrier cell according to claim 1 is inoculated with adherent uniformity device, it is characterised in that：It is described
Device body（1）Spill fixing groove is provided with above inside bottom（5）.
4. a kind of control microcarrier cell according to claim 1 is inoculated with adherent uniformity device, it is characterised in that：It is described
Micro-driving motor（15）Lower section is provided with fixed strut（16）.
5. a kind of control microcarrier cell according to claim 1 is inoculated with adherent uniformity device, it is characterised in that：It is described
Snorkel（21）With miniature gas collection air pump（27）Between pass through movable loop bar（22）Flexible connection.
6. a kind of control microcarrier cell according to claim 1 is inoculated with adherent uniformity device, it is characterised in that：It is described
Micro-driving motor（15）With paddle（14）Between pass through shaft（12）Connection.
7. a kind of control microcarrier cell according to claim 1 is inoculated with adherent uniformity device, it is characterised in that：It is described
Charge density detector（31）, charge mass detector（32）, pH value detector（33）And temperature monitor（34）Between it is logical
Cross connection partition plate（35）Connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721541278.5U CN207452141U (en) | 2017-11-17 | 2017-11-17 | A kind of control microcarrier cell is inoculated with adherent uniformity device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201721541278.5U CN207452141U (en) | 2017-11-17 | 2017-11-17 | A kind of control microcarrier cell is inoculated with adherent uniformity device |
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| CN207452141U true CN207452141U (en) | 2018-06-05 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109797102A (en) * | 2017-11-17 | 2019-05-24 | 北京中原合聚经贸有限公司 | A kind of adherent uniformity device of control microcarrier cell inoculation |
| CN111670811A (en) * | 2020-07-31 | 2020-09-18 | 广西壮族自治区农业科学院 | Constant temperature device for cell tissue culture |
| CN111979088A (en) * | 2019-05-23 | 2020-11-24 | 比欧联科供应链管理(北京)有限公司 | Breathable bag forward and reverse perfusion cell culture device and culture method |
-
2017
- 2017-11-17 CN CN201721541278.5U patent/CN207452141U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109797102A (en) * | 2017-11-17 | 2019-05-24 | 北京中原合聚经贸有限公司 | A kind of adherent uniformity device of control microcarrier cell inoculation |
| CN111979088A (en) * | 2019-05-23 | 2020-11-24 | 比欧联科供应链管理(北京)有限公司 | Breathable bag forward and reverse perfusion cell culture device and culture method |
| CN111979088B (en) * | 2019-05-23 | 2022-08-26 | 比欧联科供应链管理(北京)有限公司 | Breathable bag forward and reverse perfusion cell culture device and culture method |
| CN111670811A (en) * | 2020-07-31 | 2020-09-18 | 广西壮族自治区农业科学院 | Constant temperature device for cell tissue culture |
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Address after: Room 308, Zone C, floor 3, Xinya building, No. 63, Xisihuan North Road, Haidian District, Beijing 100142 Patentee after: Beijing Zhongyuan Heju Biotechnology Co., Ltd. Address before: 100125 No. 11 Dongfang East Road, Beijing, Chaoyang District Patentee before: BEIJING ZHONGYUAN HEJU ECONOMIC AND TRADE CO., LTD. |
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