CN207351955U - A kind of target plate - Google Patents
A kind of target plate Download PDFInfo
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- CN207351955U CN207351955U CN201721276340.2U CN201721276340U CN207351955U CN 207351955 U CN207351955 U CN 207351955U CN 201721276340 U CN201721276340 U CN 201721276340U CN 207351955 U CN207351955 U CN 207351955U
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- target
- target spot
- target plate
- spot
- quality control
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Abstract
A kind of target plate, it can be specifically Matrix-assisted laser desorption ionization target plate, its surface is divided into some pickup areas, if having dry-eye disease target spot and a Quality Control target spot in each pickup area, if Quality Control target spot is surrounded by it with the dry-eye disease target spot of pickup area.Can realize that subregion carries out Quality Control using the target plate of the utility model, eliminate the region difference present in target plate in the prior art, avoid target plate uneven surface, electric field apply uneven, sample height it is uneven etc. caused by adverse effect.
Description
Technical field
It the utility model is related to field of mass spectrometry, and in particular to a kind of target plate.
Background technology
MALD I-TOF MS (Matrix-assisted laser desorption ionization, Matrix-Assisted Laser
Desorption/Ionization Time of Flight Mass Spectrometry) it is a kind of soft ionization mass spectrum, in 20
The end of the eighties in century comes out and develops rapidly, which be not easy testing molecule by introducing substrate molecule in ionization process
Fragment is produced, solves the problems, such as non-volatile and thermal instability large biological molecule desorption ionization, is the difficult volatilization of analysis
One of important means of organic substance, particularly in terms of analysis of protein, MALDI-TOF MS have the function that irreplaceable.Mesh
Preceding MALDI-TOF MS are mainly used in Clinical microorganism Rapid identification, protein and proteomics, high molecular material is studied,
Organic molecule research etc., plays an important role in fields such as life science, biotechnology, medicament research and developments.
But in the prior art, easily there is uneven surface during making and use in sample target plate, electric field applies
Situations such as uneven, sample is just uneven, the resolution ratio and repeatability for causing spectrogram decline.
Utility model content
The utility model provides a kind of target plate, it is possible to achieve subregion carries out Quality Control, eliminates target plate institute in the prior art
Existing region difference.
A kind of target plate, the target plate surface are divided into some pickup areas, have some target spots in each pickup area.
Preferably, if having dry-eye disease target spot and at least one Quality Control target spot in each pickup area of the target plate.
Preferably, each pickup area of the target plate has a Quality Control target spot；The Quality Control target spot is by it with collection
If the dry-eye disease target spot in region surrounds.
Preferably, if the dry-eye disease target spot in each pickup area of the target plate is laid out in the rules such as square or circle
Shape；If the Quality Control target spot is in square or the circular center that it is formed with pickup area dry-eye disease target spot.
Preferably, the target plate surface is divided into some pickup areas so that the target plate, which is partitioned domain, carries out Quality Control；
The Quality Control target spot is used to carry out Quality Control to sample pre-treatments and mass spectral analysis.
Preferably, the target plate is Matrix-Assisted Laser Desorption Ionization Time of Flight target plate.
Preferably, some position correction symbols are also distributed with the target plate surface, and the position correction symbol has with each target spot
Relative position relation；The effect of the position correction symbol is, by the position where position correction symbol, according to itself and each target spot
Relative position relation can calculate the position of each target spot.
Preferably, some target spot indications are further included, each target spot indications are distinct symbols, for recognizing each target spot.
Preferably, some target spot indications are one group of line direction target spot indications and one group of column direction location mark
Symbol；A certain target spot indications in the line direction target spot indications group and a certain target in the column direction location mark symbol group
The point a certain target spot of indications Identification Simultaneously.
Preferably, location hole and/or locating slot are further included, for being coordinated with target fixing device so as to fulfill described
The accurate positionin of target plate；Bar code identification region is further included, for aiding in bar code reading device to identify the information of the target plate.
It can realize that subregion carries out Quality Control using the target plate of the utility model, eliminate present in target plate in the prior art
Region difference, avoid target plate uneven surface, electric field apply uneven, sample height it is uneven etc. caused by unfavorable shadow
Ring.
Brief description of the drawings
Fig. 1 is a kind of MALDI-TOF MS target plate schematic diagrames of embodiment of the utility model.
Embodiment
The utility model is described in further detail below by embodiment combination attached drawing.
The utility model embodiment provides a kind of target plate, as shown in Figure 1, target plate surface is divided into some pickup areas 10, target
If having dry-eye disease target spot 102 and a Quality Control target spot 101 in each pickup area 10 of plate, Quality Control target spot 101 is adopted together by it
If the dry-eye disease target spot 102 in collection region 10 surrounds.
Specifically, if the dry-eye disease target spot 102 in each pickup area 10 of target plate is laid out in square；Quality Control target spot
If 101 in the square center that it is formed with 10 dry-eye disease target spot 102 of pickup area.Such as in Fig. 1, target plate is divided into six
Pickup area 10, each pickup area 10 have 16 sample target spots 102 and a Quality Control target spot 101, wherein, 12 samples
Product target spot 102 forms the square enclosure of pickup area 10, and four sample target spots 102 are formed to be enclosed in the square of pickup area 10
And Quality Control target spot 101 is enclosed in square center.The distance between two sample target spots, which are significantly less than, in same pickup area comes
The distance between two sample target spots from different acquisition region, therefore technical staff is easy for distinguish what is formed on target plate
Six pickup areas.
In the utility model others embodiment, if dry-eye disease target spot 102 in each pickup area 10 can be with
It is laid out in the regular shapes such as circle；Quality Control target spot 101 is in the center of the regular shapes such as circle.
Matrix-assisted laser desorption ionization is mainly detected by ion gun (MALDI) and time of flight mass
Device (TOF) forms, and MALDI-TOF MS can work according to following principle：Apply about 20KV high pressures on sample target plate, profit
Absorbed with sample and substrate formed cocrystallization film, substrate molecule on the laser irradiation target plate target spot of some strength from laser
Energy, sample desorption, occurs electric charge transfer and causes ionized sample molecule, the sample of ionization is in high-voltage electricity between matrix and sample
Accelerated under field action and fly into time of flight mass detector, since the mass-to-charge ratio (m/z) of different ions is different, so arriving
Flight time t up to time of flight mass detector is also different, therefore the detection of ion can be carried out according to flight time t.Using
The reference sample known, can calibrate flight time t according to known ion massspectrum peak, complete by flight time t to matter
The conversion of lotus ratio (m/z).
MALDI-TOF is in clinical microorganism identification, microorganism pretreatment process, hardware acquisition system, at software data
Reason system can all have an impact qualification result, and carrying out quality control to above procedure is very important.Quality control can lead to
Cross a certain target spot on sample target plate and place known sample, by carrying out pre-treatment, collection and software analysis to known sample
To actual result want to compare with known results, calibration of the output results or verification are carried out according to comparison result, so as to complete whole process
Quality control.
Based on above-mentioned analysis and the utility model to the improved though of target plate, the sample target spot 102 of the present embodiment is in area
Domain property is distributed, and the Quality Control target spot 101 set by among each pickup area 10 is used for sample pre-treatments and mass spectral analysis
Carry out Quality Control.In practical operation, when being acquired to the sample target spot 102 in certain pickup area 10, first to the region
Quality Control target spot 101 is acquired, and is corrected or is verified according to the result of collection, then again to the 10 other collections of this pickup area
Point and sample target spot 102 are acquired, so as to strictly control the acquisition quality of 10 collection point of this pickup area.According to actual need
Will, the target plate product of the present embodiment covers disposable and use repeatly target plate.
The target plate surface of the present embodiment is divided into some pickup areas 10 so that target plate, which is partitioned domain, carries out Quality Control, Quality Control
Target spot 101 is used to carry out Quality Control to sample pre-treatments and mass spectral analysis, and on this basis, target plate surface is also evenly distributed with
The position correction symbol 20 of some " ten " fonts, position correction symbol 20 have relative position relation, position correction symbol 20 with each target spot
Effect be that location mark symbol 20 itself can determine the position of current target plate, further, pass through position correction and accord with 20 institutes
Position, the position of each target spot can accurately be calculated so as to fulfill to each target spot according to the relative position relation of itself and each target spot
Calibrated.Position correction symbol 20 can also be other any easily recognizable symbols or pattern.
Some target spot indications are also devised with target plate, each target spot indications are distinct symbols, if Fig. 1 is one group of line direction
" 1,2,3...12 " numerals and one group of column direction location mark accord with " A, B, C...H " letter to target spot indications；Line direction target spot mark
Show a certain target spot indications of Fu Zuzhong and a certain a certain target of target spot indications Identification Simultaneously in column direction location mark symbol group
Point.For example, line direction target spot indications " 5 " and column direction target spot indications " B " identify the sample target of the second row secondary series
Point.
The agent structure of target plate is square, but can also flexibly set location hole 301, positioning in the suitable position of target plate
Groove 302 and bar code identification region 40, such as in Fig. 1, location hole 301, locating slot 302 and bar code identification region 40 are located at target
Plate bottom.The combination of location hole 301 and locating slot 302 is used to be coordinated so as to fulfill the accurate of target plate with target fixing device
Positioning；Bar code identification region 40 is used for the relevant information for aiding in bar code reading device identification target plate.
The target plate subregion of the utility model carries out Quality Control, eliminates the interregional difference present in target plate in the prior art
It is different, avoid target plate uneven surface, electric field apply uneven, sample height it is uneven etc. caused by adverse effect.
Above content is to combine specific embodiment further description made for the present invention, it is impossible to assert this hair
Bright specific implementation is confined to these explanations.For general technical staff of the technical field of the invention, do not taking off
On the premise of from present inventive concept, some simple deduction or replace can also be made.
Claims (9)
- A kind of 1. target plate, it is characterised in that：The target plate surface is divided into some pickup areas, has some target spots in each pickup area；If there is dry-eye disease target spot and at least one Quality Control target spot in each pickup area of the target plate；If the Quality Control target spot is surrounded by it with the dry-eye disease target spot of pickup area.
- 2. target plate according to claim 1, it is characterised in that：Each pickup area of the target plate has a Quality Control target spot.
- 3. target plate according to claim 2, it is characterised in that：If the dry-eye disease target spot in each pickup area of the target plate is laid out in square or circle；If the Quality Control target spot is in square or the circular center that it is formed with pickup area dry-eye disease target spot.
- 4. target plate according to claim 1, it is characterised in that：The target plate surface is divided into some pickup areas so that the target plate, which is partitioned domain, carries out Quality Control；The Quality Control target spot is used to carry out Quality Control to sample pre-treatments and mass spectral analysis.
- 5. according to claim 1-4 any one of them target plates, it is characterised in that：The target plate is Matrix-Assisted Laser Desorption Ionization Time of Flight target plate.
- 6. according to claim 1-4 any one of them target plates, it is characterised in that：Some position correction symbols are also distributed with the target plate surface, and the position correction symbol is closed with each target spot with relative position System；The effect of the position correction symbol is, by the position where position correction symbol, according to its relative position with each target spot Relation can calculate the position of each target spot.
- 7. according to claim 1-4 any one of them target plates, it is characterised in that：Some target spot indications are further included, each target spot indications are distinct symbols, for recognizing each target spot.
- 8. target plate according to claim 7, it is characterised in that：Some target spot indications are one group of line direction target spot indications and one group of column direction location mark symbol；A certain target spot indications in the line direction target spot indications group with it is a certain in the column direction location mark symbol group The a certain target spot of target spot indications Identification Simultaneously.
- 9. according to claim 1-4 any one of them target plates, it is characterised in that：Location hole and/or locating slot are further included, for being coordinated with target fixing device so as to fulfill the accurate of the target plate Positioning；Bar code identification region is further included, for aiding in bar code reading device to identify the information of the target plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721276340.2U CN207351955U (en) | 2017-09-29 | 2017-09-29 | A kind of target plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721276340.2U CN207351955U (en) | 2017-09-29 | 2017-09-29 | A kind of target plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207351955U true CN207351955U (en) | 2018-05-11 |
Family
ID=62357178
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721276340.2U Active CN207351955U (en) | 2017-09-29 | 2017-09-29 | A kind of target plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207351955U (en) |
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2017
- 2017-09-29 CN CN201721276340.2U patent/CN207351955U/en active Active
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