CN208869595U - A kind of mass spectrum substrate target holder for detection of biological samples - Google Patents

Abstract

The utility model provides a kind of for carrying the target holder of mass spectrum substrate in detection of biological samples, target holder main body including rectangular or square, it is respectively set multiple gene substrate card slots and albumen substrate card slot in main body, the corresponding target holder of each card slot, which is successively overlapped, is equipped with mass spectrum substrate card slot first step, mass spectrum substrate card slot second step, mass spectrum substrate card slot third step.The utility model can carry out gene, albumen and microbial identification simultaneously, and there is high-throughput, substrate to select, and flexible, flatness is high, detection Mass accuracy is high.

Description

A kind of mass spectrum substrate target holder for detection of biological samples

Technical field

The utility model relates to a kind of mass spectrum substrate target holders for BIOMARK detection, pass through the spy of mass spectrum substrate target holder Kind technique and superhigh precision improve MALDI-TOF Mass Spectrometer Method Mass accuracy, to improve genetic test, microprotein mirror Fixed accuracy belongs to Mass Spectrometer Method field.

Background technique

Flight time mass spectrum starts from the 1940s, being confined to the backwardness of electronic technology at that time and instrument and equipment, instrument Resolution ratio is difficult to promote and apply less than 100.Over time with the progress of science and technology, to the 80's Mos, After the substance assistant laser desorpted ionized new method of the utility models such as Hillenkamp, by this soft ionization method and flight Time mass spectrum combination, forms a kind of new instrument: substance assistant laser desorpted ionized/flight time mass spectrum, i.e. MALDI-TOF MS(Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry), it is the novel organic mass spectrometry of a kind of soft ionization developed in recent years, by introducing substrate molecule, is made Testing molecule does not generate fragment, solves the problems, such as non-volatile and thermal instability large biological molecule desorption ionization, is point One of the important means of the organic substance of the difficult volatilization of analysis.

The principle of MALDI-TOF MS: when with the laser irradiation sample of some strength and substrate formed cocrystallization film, Matrix absorbs energy from laser, sample desorption, and electric charge transfer occurs between matrix-sample and makes ionized sample molecule, electricity From sample accelerate to fly over dirft tube under electric field action, it is different according to the flight time for reaching detector and be detected, root The ratio between quality charge according to ion (M/Z) is directly proportional to the flight time of ion to detect different ions.

MALDI TOF has become detection and identification polypeptide, protein, polysaccharide, nucleotide, glycoprotein, high polymer in recent years And the powerful tool of a variety of synthetic polymers, and it is widely used in the drug development of biotechnology and pharmacy corporation, scientific research Nuclear radiation, chemical substance and monitoring of bio-pathogen of the bioanalysis in field and chemical detection and security department etc..

MALDI-TOF MS has high sensitivity, accuracy height, high resolution, map simplicity, mass range wide and speed The features such as fast, operationally sample preparation it is easy, can milligram ammonia, extensive, parallelization and increasingly automated processing biological sample to be checked, And there is special superiority in measurement large biological molecule and synthetic high polymer application aspect.Such as measured using MALDI-TOF MS The peptide mass fingerprinting of protein digestion cracks (PSD) fragment ion map after composing (PMF), source and combines mass spectrum network data Library searching can get the sequence of polypeptide, protein.Genome single nucleotide polymorphism (SNPs) is carried out using MALDI-TOF Analysis detection, can distinguish and identify relative molecular mass up to 7,000 or so (containing more than 20 a bases), there is only 1 base difference Different DNA.It is worth pointing out that have become must be indispensable in life science proteome research by MALDI-TOF One of important key technology.

MALDI-TOF can solve in current protein science following several potential challenges, i.e., the discovery of biomarker, Molecular diagnosis research and development, protein high throughput analysis and proteomic map, but it is not limited to this, MALDI-TOF MS application Pathogenic mechanism is disclosed in the discovery of biomarker and on molecular level, to be applied to molecular diagnosis, target Treatment and personalized medicine, growth will be more completely prompted for people, develop and the rule of the vital movements such as metabolic regulation and The genesis mechanism of serious disease carries out the diagnosis prevention and treatment of disease for the mankind and new drug development provides important theoretical basis.

Traditional MALDI-TOF MS target plate application field is single, as SHIMADZU produce MALDI-TOF Axima, Target plate is served only for albumen microbial identification；The MALDI-TOF MASSARRY of AGENA production, target plate are served only for genetic test；Also There is no a kind of covering gene to detect, three kinds of the albumen microbial identification MALDI-TOF mass spectrum target plates applied.

In addition, MALDI-TOF tradition target plate, flux are fixed, albumen microorganism substrate flux has 384well, 48well, Gene substrate flux has 384well, 96well, 24well, cannot a variety of applications selection collocation, and cannot multiple target plates simultaneously into Sample, multiple laboratory technicians can not test simultaneously, and Mass Spectrometer Method Mass accuracy is low, and identification accuracy is low etc..

Utility model content

First purpose of the utility model is to provide a kind of mass spectrum substrate target holder for BIOMARK detection, other features It is:

The target holder front is rectangular or square main body, wherein the direction lead angle 1 at oblique angle is arranged at one jiao of main body, and in master The locating groove 2 for extending to main body left and right ends is respectively arranged in the top and bottom of body, positions for vacuum chamber sample introduction card slot；

Main body left part is equipped with multiple gene substrate card slots 3 in parallel, and right part is equipped with multiple albumen microorganism substrate card slots in parallel 4, and through-hole is run through in each mass spectrum substrate card slot quadrangle, to clamp substrate；

Successively overlapping is equipped with mass spectrum substrate card slot first step 5, mass spectrum to the corresponding target holder main body of substrate position from top to bottom Substrate card slot second step 6, mass spectrum substrate card slot third step 7, wherein mass spectrum substrate card slot first step 5, places mass spectrum base Piece；Mass spectrum substrate card slot second step 6 places attachment or is adhered to the non magnetic iron plate of mass spectrum substrate back；Mass spectrum substrate card Slot third step 7 is embedded in disc-shaped magnet.This set, effect are the iron plate of adsorbate spectrum substrate back, make mass spectrum substrate It is placed in target holder and achievees the purpose that fixed mass spectrum substrate.

In one embodiment, the direction lead angle 1 has 45 ° or other suitable angles for identifying main direction Degree.

In another embodiment, the locating groove 2 is the locating groove for being parallel to main body, width range 2mm- 10mm, altitude range 1mm-5mm, machining accuracy ± 0.01mm.

In another embodiment, the quantity of gene substrate card slot 3 and albumen microorganism substrate card slot 4 can be according to biological sample This detection demand setting, such as: 4+2,2+3,6+1 number combinations；

In other embodiments, the mass spectrum substrate card slot first step 5, step depth range 0.5mm-1.5mm, Machining accuracy 0.01mm, 10 μm of the flatness < of step plane, Mass Spectrometer Method Mass accuracy < 350ppm place mass spectrum substrate；

In other embodiments, the mass spectrum substrate card slot second step 6, step depth range 1mm-2mm, processing Precision 0.01mm, 20 μm of the flatness < of step plane place mass spectrum substrate behind round sheet iron plate；

In other embodiments, the mass spectrum substrate card slot third step 7, step depth range 0.3mm-1mm add Work precision 0.01mm, 30 μm of the flatness < of step plane place round sheet magnet, fixed absorption mass spectrum substrate；

Also in other embodiments, the mass spectrum substrate card slot through-hole 8, diameter 3-10mm, to facilitate clamping substrate.

In above-mentioned all embodiments, the mass spectrum substrate target holder, material characteristics are that strong but pliable in texture, flatness is good, Metal, silicon wafer, graphene may be selected.

In above-mentioned all embodiments, wherein detection albumen the corresponding body floor of mass spectrum substrate, can be set or It is not provided with third step 7.This is because protein spectrum substrate material is heavier, it is put into mass spectrum substrate card slot and is made by self gravity With being adsorbed without magnetic sheet, therefore only need the first and second step structure.In a specific embodiment, the area of three kinds of steps It can according to need and be configured adjustment.In a preferred embodiment, the size of three kinds of steps is successively One step, second step, third step.In a further preferred embodiment, overlapped centered on three kinds of steps.

In above-mentioned all embodiments, the mass spectrum substrate target holder is to be suitable for the CLIN-TOF-II clinical flight time Mass spectrometer.

Technical effect

1, the utility model can place gene substrate and albumen microorganism substrate simultaneously, and single injected sampling can carry out simultaneously Genetic test and albumen microbial identification avoid different detections from distinguishing sample introduction, save the time, single injected sampling can save 15 points Clock detects 5 times, saves the time 60 minutes within one day for one day, is suitble to high-throughput detection.

2, the utility model flux is flexible, and a target holder can place gene substrate 4 and open, and albumen microorganism substrate 2 is opened, i.e., and 4 + 2 modes can also can be arranged with 2+3,6+1 isotype, flux according to the detection demand of biological sample；

3, the utility model can more people operation of sample preparation simultaneously, a target holder is divided into the micro- life of+2 albumen of 4 gene substrates For object substrate, 6 unit substrates are divided into, sample can be prepared simultaneously with 6 laboratory technicians, printing operation, 6 units can be with one Secondary sample introduction.

4, the utility model accuracy is high, and the flatness of target holder card slot directly affects Mass Spectrometer Method Mass accuracy, thus shadow Accuracy is rung, it is accurate to greatly improve Mass Spectrometric Identification by this target holder card slot flatness < 10um, Mass Spectrometer Method Mass accuracy < 350ppm Degree.

5, the utility model target holder versatility is good, is suitble to use on a variety of mass spectrometers.It is firm new rich to be particularly suitable for Beijing It creates Biotechnology Co., Ltd and produces CLIN-TOF-II clinic time-of-flight mass spectrometer, registration certificate number: tool note in capital is quasi- 20162401065。

Detailed description of the invention

Fig. 1 mass spectrum substrate target holder floor map

Fig. 2 mass spectrum substrate target holder diagrammatic cross-section

Fig. 3 deafness standard items spectrogram

Fig. 4 microorganism detection Escherichia coli spectrogram

Fig. 5 genetic test deafness plasmid spectrogram

Specific embodiment

The utility model is described further with reference to the accompanying drawings and embodiments.

The preparation of embodiment 1, target holder

(1) it processes raw material

The plate raw material of target holder processing preferably for stainless steel, checks raw material specification, material, lot number, steel surface is without bright Aobvious concave surface and damage, surface scratch depth are not greater than 0.5mm, and should not exceed the 1/2 of the negative tolerance of the thickness of steel product.

(2) plane is crossed

Using CAD mechanical drawing program, processing and fabricating figure, sample bar, template and steel tape are crossed.When progress blanking portion To consider to shear surplus, chipping allowance when division line.

(3) plate cutting

By plasma cutting technique, milling side is carried out according to preset pattern to plate, obtains target holder main body steel sheet structure.

(4) turner of target holder main body

First rough turn, according to the step dimension that drawing is set, rough turn each step width and length stay allowance；

Smart car afterwards, according to the step dimension that drawing is set, each step width of smart car and length, 3.2 μ of surface roughness Ra m。

(5) it polishes

To the target holder first product of preparation, the first, second and third step of card slot is polished twice by CMP method, i.e. rough polishing and thin It throws.The purpose of rough polishing is the removal remaining mechanical damage of ledge surface, and the thickness within the scope of 30um is generally removed from surface.Fine polishing Purpose is the removal slight damage that polishing leaves in ledge surface for the first time and cloud defect, generally 2 are removed from surface~ 3um.There is mirrored effect by the chip surface polished twice, during Mass Spectrometer Method, reflect and take the photograph by illumination, optical path As head real-time display, the case where chip surface sample is bombarded can be directly observed, the position of laser bombardment is changed, obtained best Map.In a more specific embodiment, the chip is the silicon chip or quartz chip of single-sided polishing.

(6) it detects

Workpiece mechanical dimension is detected, after meeting drawing processing request, and step is detected by illumination, optical path bounce technique and puts down Face degree and machining accuracy, determine qualified product, and referable uses.

Embodiment 2, target holder flatness influence qualification result accuracy

With deaf gene standard items (the deaf kit of Beijing Yixin Bochuang Biotechnology Co., Ltd., product number: 1010109) for,

(1) material and method

(a) preparation:

Prepared matrix solution, the deaf UEP standard items that pre-treatment is completed, the microarray Mass Spectrometer Method core cleaned up Piece.

(b) point sample:

3 microarray Mass Spectrometer Method chips are taken, the sample well of each chip puts 0.75 μ L matrix solution, after doing naturally, Put 0.5 μ L deafness UEP standard items again, 12 repetitions, after volatilizing naturally, for use

(c) three kinds of flatness card slots:

Select three kinds of flatness card slots of mass spectrum substrate target holder, 10 μm of 1 flatness < of card slot, 20 μm of 2 flatness < of card slot, card 30 μm of 3 flatness < of slot, correspondence are put into the good microarray Mass Spectrometer Method chip of three points in three card slots, and sample introduction is put into CLIN-TOF-II flight time mass spectrum sample room.

(d) CLIN-TOF-II flight time mass spectrum parameter is set:

Turing mode:linear；

Mass Range:3000-9000；

Max Laser Rep Rate:10.0；

Power:105；

Profiles:40；

Shots:10。

(e) vacuum threshold:

As vacuum degree < 5E-6, start to detect；

(2) result and analysis

As shown in table 1,10 μm of 1 flatness < of card slot, 12 repetitions, each site peak deviation: (dark green is maximum inclined Difference)

Table 1

The results are shown in Table 1, and maximum deviation is in the position 8214.4Da, deviation 2.78Da, mass accuracy: (M-M₀)* 10⁶/M₀=(8214.4-8211.62) * 10⁶/ 8214.4=338.4ppm < 350ppm.

Wherein, mass accuracy is lower, and peak position is more accurate, and testing result accuracy is higher.

As shown in table 2,20 μm of 2 flatness < of card slot, 12 repetitions, each site peak deviation: (dark green is maximum inclined Difference)

Table 2

The results are shown in Table 2, and maximum deviation is in the position 8214.4Da, deviation 3.89Da, mass accuracy

(M-M₀)*10⁶/M₀=(8218.29-8214.4) * 10⁶/ 8214.4=473.5ppm < 500ppm.

As shown in table 3,30 μm of 3 flatness < of card slot, 12 repetitions, each site peak deviation: (dark green is maximum inclined Difference)

Table 3

The results are shown in Table 3, and maximum deviation is in the position 8214.4Da, deviation 4.26Da, mass accuracy

(M-M₀)*10⁶/M₀=(8214.4-8210.14) * 10⁶/ 8214.4=518.6ppm < 550ppm.

The above mass spectrogram result is referring to Fig. 3 deafness standard items spectrogram.

Conclusion: target holder flatness is affected to spectrogram mass accuracy, therefore, the optimal quality index of the utility model Be: 10 μm of target holder flatness <, mass accuracy < 350ppm can be improved detection of nucleic acids accuracy rate.

Embodiment 3, microbial identification

5 kinds of Clinical microorganism samples (preservation of Microbiological Lab, Beijing Yixin Bochuang Biotechnology Co., Ltd.) is selected, 20 μ l-30 μ l component I are added in each centrifuge tube (200 μ l), fall within upper tube with 1 μ l aseptic inoculation ring or pipette tips picking single bacterium In, it mixes, concussion mixes 5min.

3 repetitions of every kind of bacterial strain, mass range 2000-20000Da, acquisition data are given a mark in BE3.0 software, check confidence Spend result.

Parameter setting:

Turing mode:linear

Mass Range:2000-20000

Max Laser Rep Rate:30.0

Power:60

Profiles:100

Shots:5

Serial number	Sample number	Strain Chinese	Strain name	Confidence
					1	8739-2-A1 0001	Escherichia coli	Escherichia coli	149
2	8739-2-A2 0001	Escherichia coli	Escherichia coli	127
					3	8739-2-A3 0001	Escherichia coli	Escherichia coli	134
4	8807-B1 0001	Candida albicans bacteria complex	Candida albicans	120
					5	8807-B2 0001	Candida albicans bacteria complex	Candida albicans	108
6	8807-B3 0001	Candida albicans bacteria complex	Candida albicans	112
					7	A29-C1 0001	Staphylococcus aureus	Staphylococcus aureus	125
8	A29-C2 0001	Staphylococcus aureus	Staphylococcus aureus	123
					9	A29-C3 0001	Staphylococcus aureus	Staphylococcus aureus	135
10	A21-D1 0001	Enterococcus faecium	Enterococcus faecium	123
					11	A21-D2 0001	Enterococcus faecium	Enterococcus faecium	149
12	A21-D3 0001	Enterococcus faecium	Enterococcus faecium	142
					13	I06-E1 0001	Morganella morganii strain	Morganella morganii	114
14	I06-E2 0001	Morganella morganii strain	Morganella morganii	102
					15	I06-E3 0001	Morganella morganii strain	Morganella morganii	108

Table 4

Fig. 4 microorganism detection Escherichia coli spectrogram is shown in above-mentioned mass spectral results spectrogram production.

Selecting 5 kinds of microorganism clinical samples is respectively escherichia coli, Candida albicans bacteria complex, Staphylococcus aureus Bacterium, enterococcus faecium, morganella morganii strain can correctly identify strain name, confidence level is at 100 points by Mass Spectrometer Method More than, the higher confidence level the more credible, wherein confidence level > 25 divides, and is that qualification result is credible, 15 groups of data authentication results are just Really, accuracy 100%.

Embodiment 4, genetic test

The deaf plasmid product of selection (the deaf kit of Beijing Yixin Bochuang Biotechnology Co., Ltd., product number: 1010109) for

(1) material and method

(a) preparation:

Prepared matrix solution, the deaf plasmid product that pre-treatment is completed, the microarray Mass Spectrometer Method core cleaned up Piece.

(b) standard items are put

1 microarray Mass Spectrometer Method chip is taken, a sample well of chip intermediate region, each 0.75 μ L base of hole point are selected Matter solution after volatilizing naturally, then puts 0.5 μ L deafness standard items, stand-by after volatilizing

(c) sample is put:

In standard items sample spot peripheral region, 5 sample wells of chip are selected, each 0.75 μ L matrix solution of hole point, from After so doing, then 0.5 μ L deafness positive plasmid product is put, 5 repetitions select 5 sample wells of chip, each 0.75 μ L of hole point Matrix solution after doing naturally, then puts the deaf negative plasmid product of 0.5 μ L, 5 repetitions, after volatilizing naturally, for use

(d) sample introduction:

Mass spectrum substrate target holder is selected, correspondence is put into the microarray Mass Spectrometer Method chip put in card slot, and sample introduction is put into CLIN-TOF-II flight time mass spectrum sample room.

(e) CLIN-TOF-II flight time mass spectrum parameter is set:

Turing mode:linear；

Mass Range:3000-9000；

Max Laser Rep Rate:10.0；

Power:105；

Profiles:40；

Shots:10。

(f) vacuum threshold:

As vacuum degree < 5E-6, start to detect；

(2) deaf plasmid sample qualification result:

Table 5

Specific mass spectral results spectrogram, referring to Fig. 5 genetic test deafness plasmid spectrogram.

As shown in table 5 and Fig. 5, first is classified as serial number in table, and second is classified as catalogue number(Cat.No.), and third is classified as site information, and the 4th It is classified as genotyping result, the 5th is classified as confidence level, wherein the 5th column confidence level is divided into A, B, C, tetra- kinds of D, A is most credible.Its In the 4th column genotyping result information, represent testing result, detect 10 deaf plasmid samples, 20 positions of each deafness plasmid altogether Point, totally 200 sites, genotyping result is consistent with theoretical genotyping result, testing result accuracy 100%, therefore is based on flatness 10 μm of < of mass spectrum substrate target holder, carries out deaf pattern detection, 200 sites accuracy position 100%, this mass spectrum substrate target holder has Beneficial significant effect.

Claims (10)

1. a kind of mass spectrum substrate target holder for detection of biological samples, it is characterized in that:

The target holder front is rectangular or square main body, wherein the direction lead angle 1 at oblique angle is arranged at one jiao of main body, and in main body The locating groove 2 for extending to main body left and right ends is respectively arranged in top and bottom, positions for vacuum chamber sample introduction card slot；

Main body left part is equipped with multiple gene substrate card slots 3 in parallel, and right part is equipped with multiple albumen microorganism substrate card slots 4 in parallel, and And through-hole is run through in each mass spectrum substrate card slot quadrangle, to clamp substrate；

Successively overlapping is equipped with mass spectrum substrate card slot first step 5, mass spectrum substrate to the corresponding target holder main body of substrate position from top to bottom Card slot second step 6, mass spectrum substrate card slot third step 7, wherein mass spectrum substrate card slot first step 5, places mass spectrum substrate；Matter Substrate card slot second step 6 is composed, attachment is placed or is adhered to the non magnetic iron plate of mass spectrum substrate back；Mass spectrum substrate card slot third Step 7 is embedded in disc-shaped magnet.

2. mass spectrum substrate target holder according to claim 1, characterized in that wherein direction lead angle 1 is used to identify main direction, It has 45 ° or other suitable angles, and the locating groove 2 is the locating groove for being parallel to main body, width range 2mm-10mm, altitude range 1mm-5mm, machining accuracy ± 0.01mm.

3. mass spectrum substrate target holder according to claim 1, characterized in that wherein gene substrate card slot 3 and albumen microorganism The quantity of substrate card slot 4 can be arranged according to the detection demand of biological sample, such as: 4+2,2+3,6+1 number combinations.

4. mass spectrum substrate target holder according to claim 1, characterized in that wherein mass spectrum substrate card slot first step 5, step Depth bounds 0.5mm-1.5mm, machining accuracy 0.01mm, 10 μm of the flatness < of step plane, Mass Spectrometer Method Mass accuracy < 350ppm places mass spectrum substrate.

5. mass spectrum substrate target holder according to claim 1, characterized in that wherein mass spectrum substrate card slot second step 6, step Depth bounds 1mm-2mm, machining accuracy 0.01mm, 20 μm of the flatness < of step plane place mass spectrum substrate behind circular piece Shape iron plate.

6. mass spectrum substrate target holder according to claim 1, characterized in that wherein mass spectrum substrate card slot third step 7, step Depth bounds 0.3mm-1mm, machining accuracy 0.01mm, 30 μm of the flatness < of step plane place round sheet magnet, fixed Adsorb mass spectrum substrate.

7. mass spectrum substrate target holder according to claim 1, characterized in that wherein mass spectrum substrate card slot through-hole 8, diameter 3- 10mm, to facilitate clamping substrate.

8. mass spectrum substrate target holder according to claim 1, characterized in that the mass spectrum substrate for wherein detecting albumen is led accordingly Body bottom surface can be set or be not provided with third step 7.

9. mass spectrum substrate target holder according to claim 1, characterized in that wherein the area of three kinds of steps can according to need It is configured adjustment, the size of preferably described three kinds of steps is successively first step, second step, third step.

10. mass spectrum substrate target holder according to claim 1, characterized in that wherein mass spectrum substrate target holder is to be suitable for CLIN- TOF-II clinic time-of-flight mass spectrometer.