CN207294657U - Isolate and purify the device of 2 collagen types in chicken carcasses - Google Patents
Isolate and purify the device of 2 collagen types in chicken carcasses Download PDFInfo
- Publication number
- CN207294657U CN207294657U CN201721035872.7U CN201721035872U CN207294657U CN 207294657 U CN207294657 U CN 207294657U CN 201721035872 U CN201721035872 U CN 201721035872U CN 207294657 U CN207294657 U CN 207294657U
- Authority
- CN
- China
- Prior art keywords
- membrane
- chicken carcasses
- tank
- mixing tube
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Materials For Medical Uses (AREA)
- Peptides Or Proteins (AREA)
- Cosmetics (AREA)
Abstract
The utility model discloses a kind of device for isolating and purifying 2 collagen types in chicken carcasses, include a fluid liquid tank, one raw material liq tank, one UF membrane groove, one mixing tube, two high pressure measurement motors, one forecooler, two preheaters, one temperature controller, two check valves, two inlet control valves and two discharge control valves, to the degreasing chicken carcasses extract that is exported using mixing tube uniformly mixing raw material liquid tank and the output of fluid liquid tank liquid carbon dioxide and input UF membrane groove, protected using UF membrane grooved ring and expeditiously obtain 2 collagen types of the materials such as the victory peptides of small-molecular-weight and macromolecule.It uses environmentally friendly flow of liquid carbon dioxide body, and combine the physical method of UF membrane (UF membrane groove), expeditiously the component such as 2 collagen type of high concentration macromolecular and chondroitin, glycosaminoglycan, gucosamine, sodium hyaluronate and the Amino acid of small molecule can be obtained at the same time, not only processing procedure is simple, and it is favourable not consume the energy, environment protecting, very has economic value.
Description
Technical field
The utility model is related with chicken carcasses component extraction equipment, in more detail refers to that one kind is isolated and purified in chicken carcasses
The device of 2 collagen types.
Background technology
Shown according to reported in literature, chondroitin sulfate and gucosamine are contained in 2 collagen type structures in chicken bone
Sulfate, this two kinds of materials can maintain the pH level in joint, and can slow down anchylosis, alleviate arthritic pain
Bitterly.Also contain chondroitin and sodium hyaluronate in the bone of chicken, the health of cartilage between joint can be maintained, and human body can also be strengthened and exempted from
Epidemic disease system.2 collagen types can enter enteron aisle, maintain the integrality of enteron aisle, and then improve the effect of immune system, thus change
The health of kind digestive system.
The method that collagen is extracted in the existing bone from chicken, has and reaction is hydrolyzed with acid, basic solvent, this method uses
A large amount of solvents, it is difficult to a large amount of industrialization of continous way.Also there is the reactive mode hydrolyzed using ferment, this method is worked as using substantial amounts of water
For hydrolysising solvent, while need the heat-treated reaction for terminating ferment, during can cause the denaturation of protein, and be only
The victory peptide of small-molecular-weight, such as the component substances of gelatin (Gelatine) class.
Secondly, it is that chicken carcasses and chicken feet are utilized into a large amount of hot water of high pressure for a long time such as Chinese patent CN102028171B
Boiling, heat after hydrolysis, it is necessary to again by concentration the step of, the victory peptide and glutin of small molecule are dried, this technology
It is unable to reach the purpose of 2 collagen types of separating and purifying high-purity.United States Patent (USP) US6838440B2, is by chicken carcasses low temperature
After drying, low concentration and the chicken bone dry products of low-purity are obtained with milling by crushing, its without extraction procedures, product
Mouthfeel acceptance is low, and need to take the effect that high dose begins to reach appropriate.Chinese patent CN102690372B, is with chicken carcasses
Extract and separate goes out chondroitin sulfate, and obtains chicken bone extraction solution body with mode of heating, recycles macroreticular resin to be adsorbed, eluted
To obtain chondroitin sulfate.After it is using substantial amounts of hot cooking extraction, then with the separated sulfuric acid of substantial amounts of salt solution wash-off
Chondroitin, not only time-consuming for work flow, and the use of substantial amounts of hot water, saline solution is solvent, needs to be concentrated and dried again after separation, disappears
Consume the energy and environment protecting is bad.Chinese patent CN10398803B, is to use two kinds of enzyme water of alkali protease and compound protease
After solving the collagen component in chicken carcasses, it is deodorized through activated carbon, then is purified with film ultra-filtration filters.During need using a large amount of
Water when being solvent, process complex steps.United States Patent (USP) US4804745, and using ferment hydrolysis method of protein, obtain
To the victory peptide medicament with treatment of arthritis.United States Patent (USP) US6323319, is after utilizing ferment method for hydrolysis, coordinates adjustment pH
Value, precipitation separate 2 collagen type components in chicken bone, the method for hydrolysis of its collagen, substantially using alkali, acid or aqueous enzyme
Solution, during use a large amount of solvents, financial cost height.
In other words, the method that collagen is extracted in the existing bone from chicken, is not the 2 Collagen Type VI eggs that can not obtain high-purity
In vain, it is to have that product mouthfeel acceptance is low, the consumption energy, environment protecting is bad, process complex steps, or using a large amount of
The shortcomings of solvent, financial cost is high.
Utility model content
The main purpose of the utility model is that a kind of device for isolating and purifying 2 collagen types in chicken carcasses is provided, its
The materials such as 2 collagen types and the small molecule victory peptides of high-purity can be expeditiously isolated and purified out, not only processing procedure is simple, and not
It is favourable to consume the energy, environment protecting, very has economic value.
To reach above-mentioned purpose, the utility model uses following technical scheme:
A kind of device for isolating and purifying 2 collagen types in chicken carcasses, includes:
One fluid liquid tank, to accommodate and provide liquid carbon dioxide;
One raw material liq tank, to accommodate and provide the extract of degreasing chicken carcasses;
One UF membrane groove, connects the fluid liquid tank and raw material liq tank, is equipped with a filter membrane in it, to can be by degreasing
The extract isolation and purification of chicken carcasses goes out 2 collagen types of the materials such as the victory peptides of small-molecular-weight and macromolecule, an electricity
Heater, to heat the extract of liquid carbon dioxide and degreasing chicken carcasses;
One mixing tube, is connected between the fluid liquid tank, the raw material liq tank and the UF membrane groove, to can
Uniformly extract and the liquid carbon dioxide of mixing degreasing chicken carcasses are to input UF membrane groove;
Two high pressure measurement motors, are connected between the fluid liquid tank, the raw material liq tank and the mixing tube, to
The extract of degreasing chicken carcasses and liquid carbon dioxide can be inputted mixing tube;
One forecooler, is connected between the fluid liquid tank and the high pressure measurement motor;
Two preheaters, are connected between the two high pressure measurement motors and the mixing tube;
One temperature controller, connects the electric heater of the UF membrane groove;
Two check valves, are connected between two preheaters and the mixing tube, to make the extraction of degreasing chicken carcasses
Liquid is taken to can only flow into the mixing tube with liquid carbon dioxide;
Two inlet control valves, are respectively arranged between two check valves and the mixing tube, that can control respectively
Whether the extract of fat chicken carcasses enters the mixing tube with liquid carbon dioxide;And
Two discharge control valves, connect the UF membrane groove respectively, are big point of output can control the UF membrane groove respectively
The saturating flow liquid (P, Permeate) of the material such as the reservation liquid (R, Retentate) of sub 2 collagen types or small-molecular-weight victory peptides;
And the opening degree of the respectively import and export control valve is controlled, more controllable flow liquid and the volume flow rate ratio for retaining liquid
Example scope.
The device for isolating and purifying 2 collagen types in chicken carcasses, has further included a pressure gauge, arranged on the film point
From groove one end, to regulate and control the pressure in the UF membrane groove.
The UF membrane groove is stainless-steel tubing pillar, and the filter membrane is ceramic ultrafiltration membrane.
The filter membrane can be one of ZrO2/TiO2 of molecular weight 15kD or 50kD molecular cut off.
The mixing tube includes an inner tube and an outer tube, and said inner tube connects the raw material liq tank, the outer tube connection
The fluid liquid tank.
The beneficial effects of the utility model:It combines UF membrane (UF membrane using environmentally friendly flow of liquid carbon dioxide body
Groove) physical method, can expeditiously at the same time obtain 2 collagen type of high concentration macromolecular and chondroitin, the grape of small molecule
The components such as glycosaminoglycan, gucosamine, sodium hyaluronate and Amino acid, not only processing procedure is simple, and does not consume the energy, environment protecting very
It is good, very have economic value.
Brief description of the drawings
Fig. 1 is the system diagram of one preferred embodiment of the utility model.
Fig. 2A is the HPLC analysis collection of illustrative plates of degreasing chicken carcasses extract in one preferred embodiment of the utility model.
Fig. 2 B are that 2 collagen type of middle macromolecular after one preferred embodiment of the utility model isolates and purifies retains liquid
HPLC analyzes collection of illustrative plates.
10:Isolate and purify the device of 2 collagen types in chicken carcasses, 11:Fluid liquid tank, 12:Raw material liq tank, 13:
UF membrane groove, 14:Mixing tube, 15,16:High pressure measurement motor, 17:Forecooler, 18,19:Preheater, 20:Temperature controller,
21、22:Check valve, 23,24:Inlet control valve, 25,26:Discharge control valve, 27:Filter membrane, 28:Electric heater, 29:Inner tube,
30:Outer tube, 31:Pressure gauge.
Embodiment
Hereinafter, one preferred embodiment of the utility model is hereby lifted, and coordinates schema to be described in further detail as rear, its
In:
First, refering to Figure 1, one preferred embodiment of the utility model isolates and purifies 2 Collagen Type VI egg in chicken carcasses
White device 10, includes a fluid liquid tank 11, a raw material liq tank 12, a UF membrane groove 13, a mixing tube 14, two high pressures
15,16, one forecooler 17 of measure motor, two preheaters, 18,19, one temperature controller 20, two check valves, 21,22, two import control
Valve 23,24 processed and two discharge control valves 25,26.
Fluid liquid tank 11, is to store and can provide liquid CO 2 fluid.
Raw material liq tank 12, is the extract that can accommodate and provide degreasing chicken carcasses, degreasing chicken carcasses extract is in chicken
Skeleton add equivalent distilled water mixing after through extract, filter residue, refrigerated centrifuge remove fat constituent the step of and obtain.
UF membrane groove 13, is stainless-steel tubing pillar of the internal diameter for 0.036m~0.125m and height 1.0m, connects fluid liquid
Tank 11 and raw material liq tank 12, are equipped with a filter membrane 27 in it, are that French Novasep companies produce Carbosep M2 or M8
The ZrO2/TiO2 ceramics ultrafiltration membranes of molecular weight 15kD or 50kD molecular cut off (cut-off), to can be by degreasing chicken bone
The extract isolation and purification of frame goes out 2 collagen types of the materials such as the victory peptides of small-molecular-weight and macromolecule, an electrical heating
Device 28, is the extract for heating liquid carbon dioxide and degreasing chicken carcasses.
Mixing tube 14, is to be connected between fluid liquid tank 11, raw material liq tank 12 and UF membrane groove 13, includes an inner tube
29 and an outer tube 30, inner tube 29 be connection raw material liq tank 12, outer tube 30 is connection fluid liquid tank 11, to for equably
Extract and the liquid carbon dioxide of degreasing chicken carcasses are mixed to input UF membrane groove 13.
Two high pressure measurement motors 15,16, be connected to fluid liquid tank 11, raw material liq tank 12 and mixing tube 14 it
Between, the extract of degreasing chicken carcasses and liquid carbon dioxide can be inputted mixing tube 14.
Forecooler 17, is connected between fluid liquid tank 11 and high pressure measurement motor 15.
Two preheaters 18,19, are connected between two high pressure measurement motors 15,16 and mixing tube 14.
Temperature controller 20, is the electric heater 28 in junctional membrane separating tank 13, to control the heating of electric heater 28
Temperature.
Two check valves 21,22, are connected between two preheaters 18,19 and mixing tube 14, to make degreasing chicken carcasses
Extract and liquid carbon dioxide can only flow into mixing tube 14.
Two inlet control valves 23,24, are to be respectively arranged between two check valves 21,22 and mixing tube 14, that can distinguish
Whether the extract and liquid carbon dioxide for controlling degreasing chicken carcasses enter mixing tube 14.
Two discharge control valves 25,26, are difference junctional membrane separating tanks 13, are output can control UF membrane groove 13 respectively
The saturating flow liquid for retaining the material such as liquid or small-molecular-weight victory peptides of 2 collagen type of macromolecular.
In addition, having further included a pressure gauge 31,13 one end of UF membrane groove is provided at, in controllable UF membrane groove 13
Pressure.One temperature digital display 32, is electrically connected temperature controller 18, can show the temperature in UF membrane groove 13.And
Control the opening degree of each import and export control valve 23,24,25,26, more controllable flow liquid and the volume flow rate ratio for retaining liquid
In a certain range.
Therefore, the function mode of the device 10 for isolating and purifying 2 collagen types in chicken carcasses of the utility model and characteristic,
Effect is as follows:
Fluid liquid tank 11 is first opened, coordinates high pressure measurement motor 15, makes liquid carbon dioxide with the volume flow of 2-4L/hr
Speed, enters mixing tube 14, and the extract of the degreasing chicken carcasses in raw material liq tank 12, is to coordinate high pressure via the check valve 21
Measure motor 16, with the volume flow rate of 200-500mL/hr, enters mixing tube 14 via check valve 22, make liquid carbon dioxide with
Degreasing chicken carcasses extract uniformly mixes in mixing tube 14.Open two discharge control valves 25,26 at the same time again, regulate and control UF membrane groove
Pressure in 13 reaches the scope of 150-200psi, and the temperature in UF membrane groove 13 is to be controlled in 40-60 with temperature controller 20
DEG C scope in, and control each import and export control valve 23,24,25,26, the saturating flow liquid for exporting two discharge control valves 25,26
(material such as P, Permeate, small-molecular-weight victory peptides) is with retaining liquid (R, Retentate, the reservation of 2 collagen type of macromolecular
Liquid) volume flow rate ratio be maintained in 4.0-6.0 scopes than 1.
Base this, via the isolation and purification of UF membrane groove 13, open discharge control valve 25, collect separated flow liquid
(Permeate, P), its component are generally the victory peptide matters of small-molecular-weight, such as chondroitin (chondroitin), gucosamine
Glycan (glycosaminoglycans, GAGS), gucosamine (glucosamine), sodium hyaluronate (hyaluronic acid) and
The components such as Amino acid (amino acids).And discharge control valve 26 is opened, and collect and retain liquid (Retentate, R), its component
2 collagen type of macromolecule material of filter membrane 27 can not generally be passed through.
Hereinafter, tubing string hereby is chromatographed using BioSep-SEC-S2000 (7.8mm × 300mm, 5 μm), with HPLC quantitative analyses
Instrument, mobile phase are concentration 0.15mol/L potassium phosphate buffers (pH=4.7), and flow velocity 1.0ml/min, UV detect ripple
A length of 210nm, tubing string temperature are room temperature, quantify 2 collagen type concentration in sample, analyze degreasing chicken carcasses extract,
As shown in Figure 2 A, and reservation liquid (R) sample that 13 isolation and purification of UF membrane groove goes out is collected in comparative analysis, as shown in Figure 2 B:
The direct low temperature drying powder-processed of chicken carcasses, or be directly added into water or after other solvent extractions obtain, product can be with
Obtain whole components in chicken carcasses, such as 2 collagen types of macromolecular, the victory peptides of small molecule for example chondroitin, gucosamine,
The components such as sodium hyaluronate, Amino acid.As Fig. 2A is shown, HPLC analysis degreasing chicken carcasses extract samples, can obtain 2 type glue at the same time
The components such as the victory peptides of former albumen and small molecule.And work as chicken carcasses raw material using acid, basic solvent or ferment hydrolysis, obtain
Victory the peptides such as component such as chondroitin, sodium hyaluronate and Amino acid, the method to small molecule can not obtain 2 collagen types.Chicken bone
After frame raw material utilizes low temperature drying, comminuted powder product contains only 2 collagen type components of low concentration;Again with chemical precipitation agent such as
Trichloroacetic acid (trichloroacetic acid), after 2 collagen type of precipitation and separation, with semipermeable dialysis membrane (such as
Spectra/Por Dialysis Membrane), using in substantial amounts of solvent (such as deionized water, buffer solution), dialysis goes to clean
Matter, retains 2 collagen type components of purifying, and the method can not obtain victory peptides such as chondroitin, gucosamine, the glass of small molecule
The components such as uric acid, Amino acid.
As shown in Figure 2 B, it is from complete degreasing chicken carcasses extract, by the 2 type glue of macromolecular of high concentration and purity
Former protein ingredient, isolates and purifies in the reservation liquid (R) of UF membrane groove 13, while simultaneously can be by the small molecule of high concentration and purity
The components such as chondroitin, glycosaminoglycan, gucosamine, sodium hyaluronate and Amino acid, isolate and purify the saturating stream in UF membrane groove 13
In liquid (P).
From the foregoing, it will be observed that the utility model this isolate and purify the devices of 2 collagen types in chicken carcasses, be the liquid using environmental protection
State CO 2 fluid, and the physical method of UF membrane (UF membrane groove) is combined, it expeditiously can obtain high concentration at the same time and divide greatly
The component such as sub 2 collagen types and chondroitin, glycosaminoglycan, gucosamine, sodium hyaluronate and the Amino acid of small molecule, not only
Processing procedure is simple, and it is favourable not consume the energy, environment protecting, very has economic value.
The foregoing is merely a preferred embodiment of the utility model, when the application that the utility model cannot be limited with this is special
Sharp protection domain, the simple equivalence changes made such as according to the claim and description of the utility model are with replacing
Change, should all still fall within the range of present utility model application the scope of the claims covers protection.
Claims (5)
- A kind of 1. device for isolating and purifying 2 collagen types in chicken carcasses, it is characterised in that:Include:One accommodating and provide the fluid liquid tank of liquid carbon dioxide;One accommodating and provide the raw material liq tank of the extract of degreasing chicken carcasses;One UF membrane groove, connects the fluid liquid tank and raw material liq tank, is equipped with a filter membrane in it, to can be by degreasing chicken bone The extract isolation and purification of frame goes out 2 collagen types of the materials such as the victory peptides of small-molecular-weight and macromolecule, and one adding The electric heater of hot liquid carbon dioxide and the extract of degreasing chicken carcasses,;One mixing tube, is connected between the fluid liquid tank, the raw material liq tank and the UF membrane groove, to can be uniform Extract and the liquid carbon dioxide of degreasing chicken carcasses are mixed to input UF membrane groove;Two high pressure measurement motors, are connected between the fluid liquid tank, the raw material liq tank and the mixing tube, that can incite somebody to action The extract of degreasing chicken carcasses and liquid carbon dioxide input mixing tube;One forecooler, is connected between the fluid liquid tank and the high pressure measurement motor;Two preheaters, are connected between the two high pressure measurement motors and the mixing tube;One temperature controller, connects the electric heater of the UF membrane groove;Two check valves, are connected between two preheaters and the mixing tube, to make the extract of degreasing chicken carcasses The mixing tube is can only flow into liquid carbon dioxide;Two inlet control valves, are respectively arranged between two check valves and the mixing tube, can control degreasing chicken respectively Whether the extract of skeleton enters the mixing tube with liquid carbon dioxide;AndTwo discharge control valves, connect the UF membrane groove respectively, are output 2 types of macromolecular can control the UF membrane groove respectively The saturating flow liquid for retaining the material such as liquid or small-molecular-weight victory peptides of collagen.
- 2. the device according to claim 1 for isolating and purifying 2 collagen types in chicken carcasses, it is characterised in that:Further include There is a pressure gauge, arranged on described UF membrane groove one end, to regulate and control the pressure in the UF membrane groove.
- 3. the device according to claim 1 for isolating and purifying 2 collagen types in chicken carcasses, it is characterised in that:The film Separating tank is stainless-steel tubing pillar, and the filter membrane is ceramic ultrafiltration membrane.
- 4. the device according to claim 3 for isolating and purifying 2 collagen types in chicken carcasses, it is characterised in that:The mistake Filter membrane can be one of ZrO2/TiO2 of molecular weight 15kD or 50kD molecular cut off.
- 5. the device according to claim 1 for isolating and purifying 2 collagen types in chicken carcasses, it is characterised in that:It is described mixed Close pipe and include an inner tube and an outer tube, said inner tube connects the raw material liq tank, and the outer tube connects the fluid liquid tank.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201721035872.7U CN207294657U (en) | 2017-08-17 | 2017-08-17 | Isolate and purify the device of 2 collagen types in chicken carcasses |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201721035872.7U CN207294657U (en) | 2017-08-17 | 2017-08-17 | Isolate and purify the device of 2 collagen types in chicken carcasses |
Publications (1)
Publication Number | Publication Date |
---|---|
CN207294657U true CN207294657U (en) | 2018-05-01 |
Family
ID=62444693
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201721035872.7U Active CN207294657U (en) | 2017-08-17 | 2017-08-17 | Isolate and purify the device of 2 collagen types in chicken carcasses |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN207294657U (en) |
-
2017
- 2017-08-17 CN CN201721035872.7U patent/CN207294657U/en active Active
Similar Documents
Publication | Publication Date | Title |
---|---|---|
TWM554269U (en) | Device for decomposing Type II collagen in purified chicken skeleton | |
CN102584934B (en) | Process for preparing human immunoglobulin for intravenous injection | |
CN103073652B (en) | A kind of extracting method of spirulina polysaccharide | |
CN104861082B (en) | Method for separating polysaccharide and protein by using choline ionic liquid two-phase aqueous system | |
CN103819577B (en) | A kind of preparation method of spirulina polysaccharide | |
CN102952187B (en) | Preparation method of high-purity bovine serum albumin | |
CN104232718B (en) | The preparation method and application of dendrobium candidum antineoplastic polypeptide | |
CN110016086B (en) | Refining process of ganoderma lucidum polysaccharide | |
CN204455079U (en) | A kind of Cordyceps militaris (L.) Link. separation of fermentative broth is purified and concentrating unit | |
CN104292327A (en) | Method for extracting phycobiliprotein from spirulina | |
CN105821093A (en) | Lactobacillus plantarum exopolysaccharide and preparation method thereof | |
CN102827304A (en) | Method for extracting trametes versicolor exopolysaccharide through two-membrane process | |
CN107602719B (en) | Ganoderma lucidum fruiting body refined polysaccharide with significant auxiliary anti-tumor activity and preparation method and application thereof | |
CN1143866C (en) | Process for separating and purifying lentinan | |
CN102241767B (en) | Extraction method of bovine serum albumin | |
CN102191234A (en) | Method for separating and purifying chicken egg white lysozyme | |
CN104131055B (en) | Preparation method for phycoerythrin ACE inhibitory peptide | |
CN107674901A (en) | From the small-molecular peptides and its extracting method of the extraction of giant salamander meat bone | |
CN110256597B (en) | Method for reducing heavy metal residues in ganoderma lucidum polysaccharide by membrane method | |
CN207294657U (en) | Isolate and purify the device of 2 collagen types in chicken carcasses | |
CN104774827A (en) | Method for preparing alginate lyase from abalone internal organs | |
CN103627683A (en) | Production method for recombinant humanized superoxide dismutase | |
CN104450656A (en) | Method for preparing and purifying thrombin in rabbit blood | |
CN104387459A (en) | Industrial separation and purification method of bacterial source antibacterial peptide | |
CN106834399A (en) | A kind of Antihypertensive Peptides from Trachyostracous mussel closed shell flesh |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
GR01 | Patent grant | ||
GR01 | Patent grant |