CN207231873U - One kind analysis sample preparation apparatus - Google Patents

Abstract

The utility model provides a kind of analysis sample preparation apparatus, including：One driving unit, has a driving output shaft；Connect a movement coordinating organization of the driving output shaft；Product fixed cell as being arranged at the movement coordinating organization；When driving output shaft one first moment of torsion of output, movement coordinating organization drives sample fixed cell to perform oscillating movement；When driving second moment of torsion that output shaft exports and the first moment of torsion is reverse, movement coordinating organization drives the execution of sample fixed cell to centrifuge rotational motion.Vibration and centrifugation can be coupling in same mechanical device and realized by it, be enormously simplify the preparation process of analysis sample, greatly improved efficiency.

Description

One kind analysis sample preparation apparatus

Technical field

One kind analysis sample preparation apparatus is the utility model is related to, more particularly to one kind will can vibrate and centrifugation coupling is next real Now analyze the device of sample preparation.

Background technology

Analysis sample preparation procedure generally includes to extract and purifies two steps：The purpose of extraction in sample in will treat Survey component to be transferred in liquid as far as possible, obtain so-called extracting solution；And it is then by component to be measured in extracting solution and other to purify Component separates, and most basic purifying step is exactly to remove remaining sample in extracting solution, this is usually completed with centrifugation.

Analyze extraction process and understand that the step is above all sufficiently mixed solid and liquid, to realize component to be measured From transfer of the solid into liquid, realize that this method has very much, extracted as ultrasonic extraction, Microwave Extraction and machinery shake, And wherein using it is most wide be mechanical oscillation mode.And centrifuging is rotated by high speed, realized using centrifugal force separated Method, and a kind of purely mechanic mode.In most cases, vibrate and to centrifuge the two difference in operation larger, difference need to be used Device realize, not only add expense, it is also necessary to carry out the transfer of sample, it is very inconvenient；As can both are coupling in together Realized in one mechanical device, then can greatly simplify the preparation process of analysis sample, greatly improve efficiency.

At present, external to have the report that vibration and centrifugation function are realized using stepper motor, the method for realization is, with stepping electricity Machine is vibrated as driving source with certain frequency, amplitude reciprocating action to realize；One-directional rotation centrifuges to realize.This mode is only It can realize coplanar vibration, its frequency and angle all can be limited significantly be subject to stepper motor performance, and vibration is often not enough filled Point；In addition, the load capacity of stepper motor is small, single direction rotation speed is slow, and causing can not on processing sample size and centrifugal rotational speed Fully meet the requirement of analysis sample preparation.

Utility model content

For technical problem existing in the prior art, the purpose of this utility model is to provide a kind of analysis sample preparation dress Put, vibration and centrifugation can be coupling in same mechanical device and realized by it, enormously simplify the preparation process of analysis sample, greatly Width improves efficiency.

In order to achieve the above object, the concrete technical scheme that the utility model is taken is：

One kind analysis sample preparation apparatus, including：

One driving unit, has a driving output shaft；

Connect a movement coordinating organization of the driving output shaft；

Product fixed cell as being arranged at the movement coordinating organization；

When driving output shaft one first moment of torsion of output, movement coordinating organization drives sample fixed cell to perform oscillating movement； When driving output shaft output second moment of torsion reverse with the first moment of torsion, movement coordinating organization drive sample fixed cell perform from Heart rotational motion.

Further, the movement coordinating organization includes：

It is sheathed on a unidirectional rotary unit of the driving output shaft；

It is sheathed on an eccentric oscillating unit of the driving output shaft；

When output shaft exports the first moment of torsion, unidirectional rotary unit check, eccentric oscillating unit performs eccentric vibration；

When output shaft exports the first moment of torsion, unidirectional rotary unit performs synchronous rotary with eccentric oscillating unit.

Further, the eccentric oscillating unit includes：It is sheathed on an eccentric wheel of driving output shaft；Outside eccentric wheel One bearing in portion；One horizontal slider, has a receiving opening, which is embedded in the receiving opening；Outside horizontal slider One horizontal sliding table in portion；An at least spring is equipped between the outer wall of the horizontal slider and the inner wall of horizontal sliding table；Horizontal slider, Horizontal sliding table is supported by a supporting plane.

Further, the unidirectional rotary unit includes：One unilateral bearing；Fix an one-way shaft of the unilateral bearing outer ring Bearing ring；One horizontal sliding table supporting rack, plane is foregoing supporting plane thereon；There is horizontal sliding table supporting rack a center to lead to Hole, driving output shaft are passed through without contacting the central through hole；Horizontal sliding table supporting rack has a faying face, the faying face with it is unidirectional The inner wall of the inner ring of bearing forms static friction contact.

Further, the spring is compression spring, and when output shaft exports the first moment of torsion, spring driving horizontal slider is reciprocal Horizontal movement, when output shaft exports the second moment of torsion, spring driving horizontal slider returns to an initial position.

Further, the shock-absorbing support frame being arranged between driving unit and movement coordinating organization is further included.

Further, the driving unit is a motor.

Further, the sample fixed cell is with product disk as pipe fitting placed hole.

By taking above-mentioned technical proposal, the different directions moment of torsion of driving device output drives sample by moving coordinating organization Product disk realizes horizontal oscillations and centrifugal movement respectively, so that changeable execution mechanical shaking extraction and centrifugal clarification two in an equipment Kind function, improves integration of equipments degree, reduces sample transfer link, improves preparation efficiency.

Brief description of the drawings

Fig. 1 is the overall cross-sectional view that sample preparation apparatus is analyzed in one embodiment of the utility model.

Fig. 2 is the part section structural representation that sample preparation apparatus is analyzed in one embodiment of the utility model.

Embodiment

The following is a combination of the drawings in the embodiments of the present utility model, and the technical scheme in the embodiment of the utility model is carried out Clear, complete description.

In one embodiment, there is provided one kind analysis sample preparation apparatus, its structural representation is in Fig. 1, figure；3 conduct of motor Driving unit, is provided with shock-absorbing support frame 2 on motor 3；Movement coordinating organization 4, movement conditioning are installed on shock-absorbing support frame 2 Mechanism 4 is connected with motor 3 at the same time, and drives operating by motor 3；Sample disc 1 is installed on movement coordinating organization 4, sample will be by It is fixed in sample disc 1, is driven by movement coordinating organization 4 and vibrated and centrifuged.

It is shown in Figure 2 in detail to move coordinating organization 4, including：Load the spring 41 of card slot, which is compression spring, and quantity is One or more；Horizontal slider 42；Bearing 43；Eccentric wheel 44；Horizontal sliding table 45；Horizontal sliding table supporting rack 46；Unilateral bearing pressure ring 47；Motor fixing plate 48；Unilateral bearing 49.

When motor 3 rotates clockwise, motor 3 is moved with movable eccentric wheel 44 along 43 inner wall of bearing, promotes horizontal slider 42 to transport Dynamic, due to 49 check of unilateral bearing, horizontal sliding table 45 is static with respect to motor 3, and motor 3 need to overcome the active force of spring 41 at this time, Horizontal slider 42 is driven to do circumferential level movement along 45 direction of horizontal sliding table.Sample disc 1 is integrally fixed on horizontal sliding table 45, Sample disc 1 equally makees horizontal oscillating movement, realizes the horizontal oscillations of sample.

When 3 stop motion of motor, since horizontal slider 42 is acted on by 41 power of spring, horizontal slider 42 is set to return to level The distal end of slide unit 45, i.e. initial position, this initial position are also the Theoretical Equilibrium position in mechanical device design.

When motor 3 rotates counterclockwise, this direction of motion can depart from unilateral bearing 49, rotate, horizontal sliding table 45 There is spring 41 between horizontal slider 42, at this time, due to resistance between the components such as eccentric wheel 44, horizontal sliding table 45, horizontal slider 42 Effect, these components tend to do mass motion without separating.Then motor 3 can drive horizontal sliding table by the way that eccentric wheel 44 is overall 45th, horizontal slider 42 does counter-clockwise rotary motion together with the sample disc 1 being fixed on horizontal sliding table 45, realize sample from The heart moves.

By varying the check direction of unilateral bearing, direction of rotation can be exchanged.In addition, in some embodiments In, unilateral bearing can also be replaced with flywheel.

It should be noted that driving unit is not limited to motor or other kinds of dynamical element, such as air motor Deng.

Sample fixed cell is not limited to the sample disc of diagram, can also select other kinds of fixed structure as needed, Being capable of the fixedly secured centrifuge tube for containing sample.

Similarly, movement conditioning structure can also carry out the adjustment in a little structure, can realize and export double-direction twist moment It is converted into the movement for performing that vibration and centrifugation rotate.

Illustrate the application mode and using effect of device described by above-described embodiment below by specific preparation process.

2.0 ± 0.05g of chicken meat sample (being accurate to 0.01g) is weighed in 50mL centrifuge tubes, is separately added into suitable Buddha's warrior attendant Alkanamine, D15- amantadines, Rimantadine, D4- Rimantadines, chlorphenamine, D4- chlorphenamine standard working solutions, after mixing Standing 30min, (sample is directly weighed into the 50mL outer tubes of centrifuge tube external member and adds standard by Oscillatory Coupling centrifugal treating method Working solution).Sample of the blank sample with 20 μ g/L of parallel addition is prepared respectively, takes blank and addition sample respectively by following two kinds Method processing, referring next to national food safety standard《The survey of amantadine and Rimantadine residual quantity in animal derived food Determine liquid chromatography-tandem mass spectrometry》Machine measures on the instrument condition of exposure draft.

(1) manual handle method：1% acetic acid acetonitrile solution 20mL, vortex oscillation 3min is added, adds 2g anhydrous slufuric acids Magnesium, vortex 30s, 4000r/min centrifugation 5min.Supernatant 1mL is taken to add 50mg PSA vortexs 30s, 4000r/min centrifugation 3min.Supernatant is taken to cross upper LC-MS/MS measure after 0.22um filter membranes.

(2) automatic processing method：2g anhydrous magnesium sulfates, 1% acetic acid of 20mL are added in the 50mL pipes of centrifuge tube external member Acetonitrile mixes；150mg PSA are added in inner tube, inner tube insertion outer tube is placed in centrifugal device, setting program 1 is：Up time Pin, which rotates, realizes that 8 fonts vibrate 2min, then rotates counterclockwise and realize that 5000rpm centrifuges 3min, and program 2 is：Rotate clockwise reality Existing 8 fonts vibration 1min, then rotate counterclockwise and realize that 500rpm centrifuges 2min, operation program, after the completion of by supernatant in inner tube Upper LC-MS/MS is measured after directly crossing 0.22um filter membranes.

Amantadine difference sample preparation methods acquired results in 1 chicken of table

As seen from the above table, for amantadine, without marked difference between the matrix effect of two methods, using vibration coupling Absolute recovery when closing centrifugal treating method with quantified by external standard method is also basically identical with manual handle method, is above 95%, And with the relatively good rate of recovery and precision result can be obtained during inner mark method ration as manual handle method.To Buddha's warrior attendant For ethamine, Oscillatory Coupling centrifugal treating method also is used with external standard method without marked difference between the matrix effect of two methods Absolute recovery when quantitative and manual handle difference also not significantly, are above 75%, and with inner mark method ration when also can Obtain the relatively good rate of recovery and precision result.According to national food safety standard《Amantadine in animal derived food Measure liquid chromatography-tandem mass spectrometry of residual quantity》Isotopic Internal Standard sizing technique used by exposure draft, in this experiment The sample Oscillatory Coupling centrifugal treating method of use and manual handle method have the similar detection result of result.

Obviously, described embodiment is only the utility model part of the embodiment, instead of all the embodiments.It is based on Embodiment in the utility model, those of ordinary skill in the art are obtained all without making creative work Other embodiment, shall fall within the protection scope of the present invention.

Claims (8)

1. one kind analysis sample preparation apparatus, it is characterised in that including：

One driving unit, has a driving output shaft；

Connect a movement coordinating organization of the driving output shaft；

Product fixed cell as being arranged at the movement coordinating organization；

When driving output shaft one first moment of torsion of output, movement coordinating organization drives sample fixed cell to perform oscillating movement；Driving When output shaft exports second moment of torsion reverse with the first moment of torsion, movement coordinating organization drive sample fixed cell performs centrifugation and turns Dynamic movement.

2. analysis sample preparation apparatus as claimed in claim 1, it is characterised in that the movement coordinating organization includes：

It is sheathed on a unidirectional rotary unit of the driving output shaft；

It is sheathed on an eccentric oscillating unit of the driving output shaft；

When output shaft exports the first moment of torsion, unidirectional rotary unit check, eccentric oscillating unit performs eccentric vibration；

When output shaft exports the second moment of torsion, unidirectional rotary unit and eccentric oscillating unit synchronous rotary.

3. analysis sample preparation apparatus as claimed in claim 2, it is characterised in that the bias oscillating unit includes：It is arranged In an eccentric wheel of driving output shaft；A bearing outside eccentric wheel；One horizontal slider, has a receiving opening, the axis Hold and be embedded in the receiving opening；A horizontal sliding table outside horizontal slider；The outer wall of the horizontal slider is slided with level An at least spring is equipped between the inner wall of platform；Horizontal slider, horizontal sliding table are supported by a supporting plane.

4. analysis sample preparation apparatus as claimed in claim 3, it is characterised in that the unidirectional rotary unit includes：One is single To bearing；Fix a unilateral bearing pressure ring of the unilateral bearing outer ring；One horizontal sliding table supporting rack, plane is foregoing branch thereon Support plane；Horizontal sliding table supporting rack has a central through hole, and driving output shaft is passed through without contacting the central through hole；Horizontal sliding table Supporting rack has a faying face, which forms static friction with the inner wall of the inner ring of unilateral bearing and contact.

5. analysis sample preparation apparatus as claimed in claim 3, it is characterised in that the spring compression spring, when output shaft exports The spring drives horizontal slider reciprocating horizontal motion during the first moment of torsion, and when output shaft exports the second moment of torsion, spring driving is horizontal Sliding block returns to an initial position.

6. analysis sample preparation apparatus as claimed in claim 1, it is characterised in that further include and be arranged at driving unit and movement A shock-absorbing support frame between coordinating organization.

7. analysis sample preparation apparatus as claimed in claim 1, it is characterised in that the driving unit is a motor.

8. analysis sample preparation apparatus as claimed in claim 1, it is characterised in that the sample fixed cell is with pipe fitting Product disk as placed hole.