CN207227839U - A kind of device for producing bacteria cellulose composite material - Google Patents
A kind of device for producing bacteria cellulose composite material Download PDFInfo
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- CN207227839U CN207227839U CN201720919990.8U CN201720919990U CN207227839U CN 207227839 U CN207227839 U CN 207227839U CN 201720919990 U CN201720919990 U CN 201720919990U CN 207227839 U CN207227839 U CN 207227839U
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Abstract
It the utility model is related to a kind of device for producing bacteria cellulose composite material, including medium container, the axis of rolling, framework material, feeding rotary drum, collect rotary drum, guide roller and pressure roller, the two rows that the axis of rolling is provided parallel to inside medium container, the axis of rolling parallel interval arrangement respectively arranged, be parallel to each other dislocation between two rows of axis of rolling, framework material is first directed into inside medium container by the feeding of feeding rotary drum and guide roller, roll around and by the axis of rolling along the shaft staggered string of two rows rolling and transmitted with S-shaped track in medium container again, finally pull out medium container through pressure roller and be directed into collect rotary drum be wound collection.The utility model realizes the efficient, continuous of bacteria cellulose composite material, large-scale production, improves product composite quality, accelerates culture medium thalline and breeds and improve the utilization ratio to culture medium.
Description
Technical field
The utility model belongs to the technical field of bacteria cellulose production, is answered more particularly to one kind production bacteria cellulose
The device of condensation material.
Background technology
Bacteria cellulose (Bacterial Cellulose, abbreviation BC) is a kind of pure cellulose produced by microorganism, is had
When be commonly called as " nata de coco ", can be widely applied to food, facial mask, high-strength functional material, weaving, papermaking, artificial blood vessel, organizational project
The fields such as stent, artificial skin and medical dressing.But existing shallow tray fermentation production equipment production efficiency and low output, and machine
Tool degree is low, and labor intensity is big, it is impossible to continuous production.Therefore it is high urgently to develop a kind of yield, and can high efficiency continuously production
Novel fermentation device.
In terms of medical dressing, hygrometric state bacteria cellulose is easily broken makes in use since mechanical strength is inadequate
Into inconvenient for use, such as to reach mechanical property same with absorbent gauze, then require cellulose membrane to reach certain thickness, this can increase
The hardness of film, patch skin are decreased obviously.
Existing prepare bacteria cellulose film composite material is to utilize static culture technology, including will be needed fine with bacterium
The compound material of dimension element, which is placed in nutrient solution, to be co-cultured, by impregnating bacteria cellulose film and organic matter macromolecule or inorganic
Thing doped and compounded, it is inefficient；And since cellulose is made only in media surface, using quiescent culture method it is compound up
Cellulose is few, and skewness, it is impossible to which covering gauze etc. needs most of gap of compound material, and cultivation cycle is very
It is long.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of device for producing bacteria cellulose composite material, real
Existing the efficient, continuous of bacteria cellulose composite material, large-scale production, improve product composite quality, and it is numerous to accelerate culture medium thalline
Grow and improve the utilization ratio to culture medium.
The utility model solves technical solution used by its technical problem, and to be to provide a kind of production bacteria cellulose compound
The device of material, including medium container, the axis of rolling, framework material, feeding rotary drum, collection rotary drum, guide roller and pressure roller, it is described
The two rows that the axis of rolling is provided parallel to inside medium container, the axis of rolling parallel interval arrangement respectively arranged, two rows of axis of rolling
Between be parallel to each other dislocation, the framework material is first directed into inside medium container by the feeding of feeding rotary drum and guide roller,
Rolled again along the shaft staggered string of two rows rolling around and by the axis of rolling and transmitted in medium container with S-shaped track, finally pulled out
Medium container, which passes through pressure roller and is directed into collection rotary drum, is wound collection.
As a kind of preferred embodiment of the utility model, the medium container is internally provided with heating plate, bottom is set
There is tap hole, the side wall of medium container is equipped with blow vent, pH meter socket, dissolved oxygen electrode socket, thermometer socket, acid ＆ alkali liquid and adds
Add mouth, Nutritional supplements mouth, feed liquid highly to visit axis and feed inlet, pH electrodes, the dissolved oxygen electrode are installed in the pH meter socket
Dissolved oxygen electrode is installed in socket, thermometer probe is installed in the thermometer socket, is installed inside the medium container
There is feed liquid Liquid level test probe.
As the utility model another preferred embodiment, the axis of rolling is arranged to inside medium container
The two rows of lower horizontal parallel, the axis of rolling that the framework material is vertically staggeredly gone here and there around upper and lower interlaced arrangement.
Improved as to the further of the above embodiment, the feed liquid Liquid level of the medium container is upper and lower two
Between row's axis of rolling.
As the utility model another preferred embodiment, the axis of rolling is arranged to left inside medium container
Right vertical parallel two rows, the axis of rolling that the framework material is staggeredly gone here and there around left and right interlaced arrangement in the horizontal direction.
Improved as to the further of the above embodiment, the high out-feed liquid liquid of the axis of rolling of part on the two rows axis of rolling
Position.
As the utility model another preferred embodiment, the feeding rotary drum and collection rotary drum are oppositely arranged respectively
In medium container both sides, the guide roller and pressure roller are correspondingly arranged in the opposite sides of medium container and hold positioned at culture medium
The top edge of device.
As the utility model another preferred embodiment, the framework material is membrane material or the spinning with mesh
Cloth material, the pore size of the mesh is 0.05mm-10mm.
As the utility model another preferred embodiment, the medium container is closed container or top
Opened type container.
As the utility model another preferred embodiment, the linear velocity of framework material transmission for 10~
280m/h.Beneficial effect
First, framework material is by feeding rotary drum and collects rotary drum hair receipts, shaft staggered by rolling inside medium container
String is transmitted around and with S-shaped track, and the characteristic easily wound using bacteria cellulose, is twined by the movement to move in circles of framework material
Around bacterium-adsorbing fibres element, uniform and strong bonded bacteria cellulose film, gained can be formed in framework material both sides
Product quality is high；
Second, framework material and the compound automation of bacteria cellulose, continuous production are realized, improves production efficiency,
Manual labor's intensity is reduced, equipment is simple, reduces production cost, is advantageously implemented large-scale industrial production；
3rd, by controlling the transfer rate of the axis of rolling, the static training in Asia to thalline can be realized in medium container
Support, framework material can ceaselessly to replace in liquid medium within and air, can either liquid culture medium provide it is enough molten
Oxygen is solved, promotes the quick breeding of thalline in culture medium and produces cellulose, so that connecing beneficial to framework material and bacteria cellulose
Touch winding；Meanwhile be conducive to have been fixed in the thalline on framework material and can not only touch nutrition, but also oxygen is adequately exposed to,
So as to promote thalline amount reproduction and eccrine fiber element, bacteria cellulose material is efficiently prepared.Many factors promote bacterial fibers
The production efficiency of cellulosic material greatly promotes；
4th, framework material is arranged inside medium container in continuous S-shaped, takes full advantage of the sky of medium container
Between, the production area of bacteria cellulose composite material is substantially increased, improves the service efficiency to culture medium, is conducive to improve
Yield；
5th, bacteria cellulose composite material is prepared by the way that framework material and bacteria cellulose are compound, the bacterium of preparation is fine
The plain film composite material of dimension remains the nanometer tridimensional network of bacteria cellulose uniqueness, while has saved the use of bacteria cellulose
Amount, has the advantages that high water absorption and moisture holding capacity, high-crystallinity, high polymerization degree, high intensity and good biocompatibility；Can
The problems such as to solve poor traditional dressing absorbency, adhesion wound, product can be widely applied to food, facial mask, wound dressing, artificial
The fields such as skin, weaving paper making raw material.
Brief description of the drawings
Fig. 1 is set in a row for the utility model axis of rolling level, the signal of the Facad structure of medium container opening embodiment
Figure.
Fig. 2 is the side structure schematic diagram of Fig. 1 axis of rolling arrangement.
Fig. 3 is the overlooking the structure diagram of Fig. 1 axis of rolling arrangement.
Fig. 4 is set in a row for the utility model axis of rolling level, the structure diagram of medium container closing embodiment.
Fig. 5 is vertically set in a row for the utility model axis of rolling, the structure diagram of medium container opening embodiment.
Fig. 6 is the side structure schematic diagram of Fig. 5 axis of rolling arrangement.
Fig. 7 is the overlooking the structure diagram of Fig. 5 axis of rolling arrangement.
Fig. 8 is vertically set in a row for the utility model axis of rolling, the structure diagram of medium container closing embodiment.
Fig. 9 is set in a row for the utility model axis of rolling level, the structure diagram of framework material formation loop.
Embodiment
With reference to specific embodiment, the utility model is expanded on further.It is to be understood that these embodiments are merely to illustrate this
Utility model rather than limitation the scope of the utility model.In addition, it should also be understood that, reading the content of the utility model instruction
Afterwards, those skilled in the art can make various changes or modifications the utility model, and such equivalent forms equally fall within this Shen
Please the appended claims limited range.
A kind of device for producing bacteria cellulose composite material as shown in figs 1-9, including medium container 1, the axis of rolling
11st, framework material 13, feeding rotary drum 15, collection rotary drum 16, guide roller 17 and pressure roller 10.
Medium container 1 is closed cuboid or opened type rectangular parallelepiped structure, and open container is placed in ten thousand grades of 1-10's
In dust proof workshop.Medium container 1 is internally provided with heating plate 2, bottom is equipped with tap hole 14, and the side wall of medium container 1 is equipped with
Blow vent 3, pH meter socket 4, dissolved oxygen electrode socket 5, thermometer socket 6, acid ＆ alkali liquid adding mouth 7, Nutritional supplements mouth 8, feed liquid are high
Degree visits axis 9 and feed inlet 12.PH electrodes are installed in pH meter socket 4, dissolved oxygen electrode, temperature are installed in dissolved oxygen electrode socket 5
Thermometer probe is installed in meter socket 6, feed liquid Liquid level test probe is installed inside medium container 1.
Feeding rotary drum 15 and collection rotary drum 16 are relatively arranged on 1 both sides of medium container, guide roller 17 and pressure roller 10 respectively
It is correspondingly arranged in the opposite sides of medium container 1 and positioned at the top edge of medium container 1.
The two rows that the axis of rolling 11 is provided parallel to inside medium container 1, can be pacified by the side wall of medium container 1
Dress, opened type container can also be hung on outside container.11 parallel interval of the axis of rolling respectively arranged is arranged, between two rows of axis of rolling 11
Be parallel to each other dislocation.One kind is preferable to provide mode：The axis of rolling 11 is arranged to horizontal parallel up and down inside medium container 1
Two rows, the feed liquid Liquid level of medium container 1 is between upper and lower two rows of axis of rolling 11, and framework material 13 is by feeding rotary drum
15 feedings and guide roller 17 are directed into inside medium container 1, vertically interlock along two rows of axis of rolling 11 and go here and there around and pass through rolling
Axis 11, which is rolled, to be transmitted in medium container 1 with S-shaped track, pulls out medium container 1 through pressure roller 10 and be directed into receipts
Collection rotary drum 16 is wound collection；Another kind is preferable to provide mode：The axis of rolling 11 is arranged to left inside medium container 1
Right vertical parallel two rows, the high out-feed liquid liquid level of the axis of rolling 11 of part, framework material 13 are turned by feeding on two rows of axis of rolling 11
15 feeding of drum and guide roller 17 are directed into inside medium container 1, are horizontally staggered along two rows of axis of rolling 11 and go here and there around and pass through rolling
Moving axis 11, which is rolled, to be transmitted in medium container 1 with S-shaped track, pulls out medium container 1 through pressure roller 10 and be directed into
Collect rotary drum 16 and be wound collection.
Framework material 13 by organic matter and or inorganic matter form, including polysaccharide, cellulose, cellulose derivative, fine jade
Fat, seaweed acids, hyaluronic acid, chitin, chitosan, chitosan derivatives, timber, starch, polypeptide, protein, gelatin, glue
Original, silk, wool, polyglutamic acid, high molecular polymer, silica gel, rubber, plastics, terylene, polypropylene fibre, nylon, Orlon, polyethylene,
Polyvinyl alcohol, polyvinyl alcohol, polytetrafluoroethylene (PTFE), polyacrylamide, gauze, cotton, paper, silica, glass, ceramics,
One or more combination in boccaro, metal, metal salt, metal oxide.Framework material 13 is for membrane material or with mesh
Woven fabric material, the pore size of mesh are 0.05mm-10mm, shape can be circular, square, triangle, ellipse, heart,
Quincunx, diamond shape or pentalpha.The wire velocity control that framework material 13 transmits is 10~280m/h.
Specific embodiment is as follows：
Embodiment 1
As shown in Figs. 1-3, medium container 1 is open cuboid container, a length of 200 meters, width be 2 meters, it is 3 meters a height of, hold
The two rows axis of rolling 11 is parallel in device respectively 1000, and the distance of 11 axle center of the axis of rolling in-between is 2.5 meters, and left and right rolls
The distance between 11 axle center of axis is 0.2 meter, a diameter of 0.1 meter of roller bearing.
(1) Spawn incubation
By acetobacter xylinum access 300mL fluid nutrient mediums (per in 1L water, glucose 400g, yeast extract 60g, tryptose
Peptone 100g, citric acid 1.15g, Na2HPO42.7g, pH5.0,115 DEG C of sterilizing 30min) spread cultivation, in 30 DEG C, 160r/min conditions
It is spare after lower shaking table culture or quiescent culture 12h；
(2) prepared by the fermentation of bacteria cellulose composite membrane
Using polyester fiber as framework material 13, in use, first by the nutrient solution that high-temp steam sterilizing is crossed from 12 note of feed inlet
Enter in medium container 1, then by the way that the fluid nutrient medium of the bacterial strain containing production is transferred in medium container 1, then motor
Rotated with the speed of 200m/h and drive framework material 13 to carry out disturbance culture 25h in the medium, you can in 13 table of framework material
Face continuously and uniformly forms certain thickness bacteria cellulose film composite material.Suitable speed is determined, when thickness is met the requirements
Afterwards, that is, reach to collect and carry out rotating collection on rotary drum 16, it is pure also into being carried out into the processing pond containing sodium hydroxide solution
Change is handled.
(3) material process
The bacteria cellulose material of preparation is removed from collection rotary drum 16, after distilled water repeatedly rinses, is then soaked in
In the NaOH solution of 0.5wt%, 70 DEG C of water bath processing 30min, make bacteria cellulose composite membrane it is white it is translucent after, first
Washed 4~5 times with the aqueous solution of the acetic acid containing 0.5mol/L, then washed repeatedly to neutrality with pure water, answered up to bacteria cellulose
Close film product.
Embodiment 2
(1) Spawn incubation
Gluconate pyracetobacillus seed liquor is linked into 300L fluid nutrient mediums by 10% access amount (per in 1L water,
5g, pH4.5,115 DEG C of maltose 100g, peptone 3g, yeast extract sterilizing 30min；Or fructose 160g, yeast extract 5g, pancreas
Peptone 5g, citric acid 1.15g, Na2HPO41L, pH5.0,115 DEG C of 2.7g, water sterilizing 30min), bioreactor control exists
Under 30 DEG C of constant temperatures；
(2) prepared by the fermentation of bacteria cellulose composite membrane
Using chintz as framework material 13, the fluid nutrient medium of the bacterial strain containing production prepared by step (1) is transferred to Fig. 1
In the medium container 1 of type, then framework material 13 carries out disturbance culture with the speed of 280m/h, in 30 DEG C of dynamic cultivation 18h
Afterwards, you can obtain bacteria cellulose/calico printing composite membrane；
(3) material process
Bacteria cellulose material will be prepared to remove from collection rotary drum 16, after distilled water repeatedly rinses, be then soaked in
In the NaOH solution of 1wt%, 80 DEG C of water bath processing 100min, make bacterial cellulose stent composite membrane it is white it is translucent after i.e.
Can, first washed 4~5 times with the aqueous solution of the acetic acid containing 0.5mol/L, then washed repeatedly to neutrality with pure water, then refrigerate, i.e.,
Obtain bacterial cellulose stent composite membrane product.
Embodiment 3
(1) Spawn incubation
Gluconate pyracetobacillus seed liquor is linked into 300L fluid nutrient mediums by 10% access amount (per in 1L water,
5g, pH4.5,115 DEG C of maltose 100g, peptone 3g, yeast extract sterilizing 30min；Or fructose 160g, yeast extract 5g, pancreas
Peptone 5g, citric acid 1.15g, Na2HPO41L, pH5.0,115 DEG C of 2.7g, water sterilizing 30min), bioreactor control exists
Under 30 DEG C of constant temperatures；
(2) prepared by the fermentation of bacteria cellulose composite membrane
Using hospital gauze as framework material 13, the fluid nutrient medium of the bacterial strain containing production prepared by step (1) is transferred to Fig. 1
In the medium container 1 of type, framework material 13 obtains cellulose/gauze composite material with the speed fermentation 48h of 104m/h；
(3) material process
Bacteria cellulose material will be prepared to remove from collection rotary drum 16, after distilled water repeatedly rinses, be then soaked in
In the NaOH solution of 1wt%, 80 DEG C of water bath processing 100min, make bacterial cellulose stent composite membrane it is white it is translucent after i.e.
Can, first washed 4~5 times with the aqueous solution of the acetic acid containing 0.5mol/L, then washed repeatedly to neutrality with pure water, then refrigerate, i.e.,
Obtain bacterial cellulose stent composite membrane product.
Embodiment 4
(1) Spawn incubation
Gluconate pyracetobacillus seed liquor is linked into 300L fluid nutrient mediums by 10% access amount (per in 1L water,
5g, pH4.5,115 DEG C of maltose 100g, peptone 3g, yeast extract sterilizing 30min；Or fructose 160g, yeast extract 5g, pancreas
Peptone 5g, citric acid 1.15g, Na2HPO41L, pH5.0,115 DEG C of 2.7g, water sterilizing 30min), bioreactor control exists
Under 30 DEG C of constant temperatures；
(2) prepared by the fermentation of bacteria cellulose composite membrane
Using nylon fibre as framework material 13, the fluid nutrient medium of the bacterial strain containing production prepared by step (1) is transferred to Fig. 1
In the medium container 1 of type, then framework material 13 carries out disturbance culture with the speed of 100m/h, in 30 DEG C of dynamic cultivation 50h
Afterwards, you can obtain bacteria cellulose/polyamide fibre composite membrane；
(3) material process
Bacteria cellulose material will be prepared to remove from collection rotary drum 16, after distilled water repeatedly rinses, be then soaked in
In the NaOH solution of 1wt%, 80 DEG C of water bath processing 100min, make bacterial cellulose stent composite membrane it is white it is translucent after i.e.
Can, first washed 4~5 times with the aqueous solution of the acetic acid containing 0.5mol/L, then washed repeatedly to neutrality with pure water, then refrigerate, i.e.,
Obtain bacterial cellulose stent composite membrane product.
Embodiment 5
As shown in figure 4, medium container 1 for closing cuboid container, a length of 200 meters, width be 2 meters, 3 meters a height of, container
The interior two rows axis of rolling 11 is parallel respectively 1000, and the distance of 11 axle center of the axis of rolling in-between is 2.5 meters, the left and right axis of rolling
The distance between 11 axle center are 0.2 meter, a diameter of 0.1 meter of roller bearing.
(1) Spawn incubation
Gluconate pyracetobacillus seed liquor is linked into 300L fluid nutrient mediums by 10% access amount (per in 1L water,
5g, pH4.5,115 DEG C of maltose 100g, peptone 3g, yeast extract sterilizing 30min；Or fructose 160g, yeast extract 5g, pancreas
Peptone 5g, citric acid 1.15g, Na2HPO41L, pH5.0,115 DEG C of 2.7g, water sterilizing 30min), bioreactor control exists
Under 30 DEG C of constant temperatures；
(2) prepared by the fermentation of bacteria cellulose composite membrane
Using nylon fibre as framework material 13, the fluid nutrient medium of the bacterial strain containing production prepared by step (1) is transferred to Fig. 4
In the medium container 1 of type, then framework material 13 carries out disturbance culture with the speed of 100m/h, in 30 DEG C of dynamic cultivation 50h
Afterwards, you can obtain bacteria cellulose/polyamide fibre composite membrane；
The medium container 1 was passed through air filtering, air mass flow 1Nm using air compressor machine during the fermentation3/h。
Embodiment 6
(1) Spawn incubation
Gluconate pyracetobacillus seed liquor is linked into 300L fluid nutrient mediums by 10% access amount (per in 1L water,
5g, pH4.5,115 DEG C of maltose 100g, peptone 3g, yeast extract sterilizing 30min；Or fructose 160g, yeast extract 5g, pancreas
Peptone 5g, citric acid 1.15g, Na2HPO41L, pH5.0,115 DEG C of 2.7g, water sterilizing 30min), bioreactor control exists
Under 30 DEG C of constant temperatures；
(2) prepared by the fermentation of bacteria cellulose composite membrane
Using cellulose fibre as framework material 13, the fluid nutrient medium of the bacterial strain containing production prepared by step (1) is transferred to
In Fig. 1 types medium container 1, then framework material 13 carries out disturbance culture with the speed of 100m/h, in 30 DEG C of dynamic cultivation 50h
Afterwards, you can obtain composite membrane；
(3) material process
Bacteria cellulose material will be prepared to remove from collection rotary drum 16, after distilled water repeatedly rinses, be then soaked in
In the NaOH solution of 1wt%, 80 DEG C of water bath processing 100min, make bacteria cellulose skeleton composite membrane it is white it is translucent after i.e.
Can, first washed 4~5 times with the aqueous solution of the acetic acid containing 0.5mol/L, then washed repeatedly to neutrality with pure water, then refrigerate, i.e.,
Obtain bacterial cellulose stent composite membrane product.
Embodiment 7
(1) Spawn incubation
Gluconate pyracetobacillus seed liquor is linked into 300L fluid nutrient mediums by 10% access amount (per in 1L water,
5g, pH4.5,115 DEG C of maltose 100g, peptone 3g, yeast extract sterilizing 30min；Or fructose 160g, yeast extract 5g, pancreas
Peptone 5g, citric acid 1.15g, Na2HPO41L, pH5.0,115 DEG C of 2.7g, water sterilizing 30min), bioreactor control exists
Under 30 DEG C of constant temperatures；
(2) prepared by the fermentation of bacteria cellulose composite membrane
Using protein fibres such as hospital gauze or wools as framework material 13, contain production bacterial strain by prepared by step (1)
Fluid nutrient medium be transferred in Fig. 1 types medium container 1, then framework material 13 carries out disturbance training with the speed of 100m/h
Support, during which, periodically add nano silver material into culture medium, the addition of nano silver material can be fed-batch type addition or
Continous way is added, after 30 DEG C of dynamic cultivation 50h, you can obtains Ag-carrying bacterial cellulose/gauze composite membrane or Ag-carrying bacterial is fine
Tie up element/wool composite membrane.
Embodiment 8
(1) Spawn incubation
Gluconate pyracetobacillus seed liquor is linked into 300L fluid nutrient mediums by 10% access amount (per in 1L water,
5g, pH4.5,115 DEG C of maltose 100g, peptone 3g, yeast extract sterilizing 30min；Or fructose 160g, yeast extract 5g, pancreas
Peptone 5g, citric acid 1.15g, Na2HPO41L, pH5.0,115 DEG C of 2.7g, water sterilizing 30min), bioreactor control exists
Under 30 DEG C of constant temperatures；
(2) prepared by the fermentation of bacteria cellulose composite membrane
Using protein fibres such as hospital gauze or wools as framework material 13, contain production bacterial strain by prepared by step (1)
Fluid nutrient medium be transferred in Fig. 1 types medium container 1, then framework material 13 carries out disturbance training with the speed of 100m/h
Support, during which, starch, gelatin or micro-nano SiO are periodically added into culture medium2Material, after 30 DEG C of dynamic cultivation 50h, you can
Bacteria cellulose/gauze/starch composite membrane is obtained, either bacteria cellulose/wool/starch composite membrane or bacteria cellulose/yarn
Cloth/synthesized thin film, either bacteria cellulose/wool/synthesized thin film or bacteria cellulose/gauze/SiO2Composite membrane,
Or bacteria cellulose/wool/SiO2Composite membrane.The addition of nano silver material can be fed-batch type addition or continous way
Addition.
Embodiment 9
Medium container 1 as illustrated in figs. 5-7 is open cuboid container, a length of 200 meters, width be 2 meters, it is 3 meters a height of, hold
The two rows of roller bearings in left and right are parallel in device respectively 15, and the distance of roller bearing axle center in-between is 0.2 meter, between the roller bearing axle center of left and right
Distance is 195 meters, a diameter of 0.1 meter of roller bearing.
(1) Spawn incubation
Gluconate pyracetobacillus seed liquor is linked into 300L fluid nutrient mediums by 10% access amount (per in 1L water,
5g, pH4.5,115 DEG C of maltose 100g, peptone 3g, yeast extract sterilizing 30min；Or fructose 160g, yeast extract 5g, pancreas
Peptone 5g, citric acid 1.15g, Na2HPO41L, pH5.0,115 DEG C of 2.7g, water sterilizing 30min), bioreactor control exists
Under 30 DEG C of constant temperatures；
(2) prepared by the fermentation of bacteria cellulose composite membrane
Using hospital gauze as framework material 13, the fluid nutrient medium of the bacterial strain containing production prepared by step (1) is transferred to Fig. 5
In type medium container 1, then framework material 13 carries out disturbance culture with the speed of 100m/h, is cultivated respectively in 30 DEG C of dynamics
After 59h, you can obtain bacteria cellulose/calico printing composite membrane.
Embodiment 10
Medium container 1 as shown in Figure 8 for closing cuboid container, a length of 200 meters, width be 2 meters, 3 meters a height of, container
Interior two rows of roller bearings in left and right are parallel respectively 15, and the distance of roller bearing axle center in-between is 0.2 meter, between the roller bearing axle center of left and right away from
From for 195 meters, a diameter of 0.1 meter of roller bearing.
(1) Spawn incubation
Gluconate pyracetobacillus seed liquor is linked into 300L fluid nutrient mediums by 10% access amount (per in 1L water,
5g, pH4.5,115 DEG C of maltose 100g, peptone 3g, yeast extract sterilizing 30min；Or fructose 160g, yeast extract 5g, pancreas
Peptone 5g, citric acid 1.15g, Na2HPO41L, pH5.0,115 DEG C of 2.7g, water sterilizing 30min), bioreactor control exists
Under 30 DEG C of constant temperatures；
(2) prepared by the fermentation of bacteria cellulose composite membrane
Using nylon fibre as framework material 13, the fluid nutrient medium of the bacterial strain containing production prepared by step (1) is transferred to Fig. 8
In type medium container 1, then framework material 13 carries out disturbance culture with the speed of 100m/h, after 30 DEG C of dynamic cultivation 59h,
Bacteria cellulose/polyamide fibre composite membrane can be obtained.
The medium container 1 was passed through air filtering, air mass flow 1Nm using air compressor machine during the fermentation3/h。
Embodiment 11
Medium container 1 as shown in Figure 9 for closing cuboid container, a length of 200 meters, width be 2 meters, 3 meters a height of, container
Interior two rows of roller bearings in left and right are parallel respectively 15, and the distance of roller bearing axle center in-between is 0.2 meter, between the roller bearing axle center of left and right away from
From for 195 meters, a diameter of 0.1 meter of roller bearing.
(1) Spawn incubation
Gluconate pyracetobacillus seed liquor is linked into 300L fluid nutrient mediums by 10% access amount (per in 1L water,
5g, pH4.5,115 DEG C of maltose 100g, peptone 3g, yeast extract sterilizing 30min；Or fructose 160g, yeast extract 5g, pancreas
Peptone 5g, citric acid 1.15g, Na2HPO41L, pH5.0,115 DEG C of 2.7g, water sterilizing 30min), bioreactor control exists
Under 30 DEG C of constant temperatures；
(2) prepared by the fermentation of bacteria cellulose composite membrane
Using nylon fibre net/polyester fiber net as framework material 13, the liquid of the bacterial strain containing production prepared by step (1) is trained
Group-transfer is supported into Fig. 9 types medium container 1, then framework material 13 carries out disturbance culture with the speed of 100m/h.In the early stage
During production, due to material skeleton also no attachment BC, do not have to directly collection, but material skeleton by the roller bearing on the left side from upper
Side raps around to the starting roller bearing on the right, forms the circuit of closing to produce.When adhering to successfully BC on framework material, it is possible to will
The roller bearing on the left side is transformed into collect and is harvested on roller bearing.After dynamic cultivation 100h is circulated in 30 DEG C of culture mediums, you can it is fine to obtain bacterium
Tie up element/polyamide fibre composite membrane.
Claims (10)
1. a kind of device for producing bacteria cellulose composite material, including medium container (1), the axis of rolling (11), framework material
(13), feeding rotary drum (15), collection rotary drum (16), guide roller (17) and pressure roller (10), it is characterised in that：The axis of rolling (11)
The two rows being provided parallel to inside medium container (1), the axis of rolling respectively arranged (11) parallel interval arrangement, two rows of axis of rolling
(11) be parallel to each other dislocation between, and the framework material (13) is directed into training by (15) feeding of feeding rotary drum and guide roller (17)
Support that based containers (1) are internal, staggeredly go here and there along two rows of axis of rolling (11) and roll with S-shaped track in culture medium around and by the axis of rolling (11)
Transmission in container (1), pull out medium container (1) through pressure roller (10) and be directed into and collect rotary drum (16) and be wound receipts
Collection.
A kind of 2. device for producing bacteria cellulose composite material according to claim 1, it is characterised in that：The culture
Based containers (1) are internally provided with heating plate (2), bottom is equipped with tap hole (14), and the side wall of medium container (1) is equipped with blow vent
(3), pH meter socket (4), dissolved oxygen electrode socket (5), thermometer socket (6), acid ＆ alkali liquid adding mouth (7), Nutritional supplements mouth (8),
Feed liquid highly visits axis (9) and feed inlet (12), and pH electrodes, the dissolved oxygen electrode socket (5) are provided with the pH meter socket (4)
In dissolved oxygen electrode is installed, thermometer probe is installed, peace inside the medium container (1) in the thermometer socket (6)
Equipped with feed liquid Liquid level test probe.
A kind of 3. device for producing bacteria cellulose composite material according to claim 1 or 2, it is characterised in that：It is described
The axis of rolling (11) is arranged to the two rows of upper and lower horizontal parallel inside the medium container (1), and the framework material (13) is along vertical
The axis of rolling (11) that direction is staggeredly gone here and there around upper and lower interlaced arrangement.
A kind of 4. device for producing bacteria cellulose composite material according to claim 3, it is characterised in that：The culture
The feed liquid Liquid level of based containers (1) is between upper and lower two rows of axis of rolling (11).
A kind of 5. device for producing bacteria cellulose composite material according to claim 1 or 2, it is characterised in that：It is described
The axis of rolling (11) is arranged to the vertical parallel two rows in left and right inside the medium container (1), and the framework material (13) is along level
The axis of rolling (11) that direction is staggeredly gone here and there around left and right interlaced arrangement.
A kind of 6. device for producing bacteria cellulose composite material according to claim 5, it is characterised in that：The two rows
The high out-feed liquid liquid level of the axis of rolling (11) of part on the axis of rolling (11).
A kind of 7. device for producing bacteria cellulose composite material according to claim 1, it is characterised in that：The feeding
Rotary drum (15) and collection rotary drum (16) are relatively arranged on medium container (1) both sides, the guide roller (17) and pressure roller respectively
(10) it is correspondingly arranged in the opposite sides of medium container (1) and positioned at the top edge of medium container (1).
A kind of 8. device for producing bacteria cellulose composite material according to claim 1, it is characterised in that：The skeleton
Material (13) is membrane material or the woven fabric material with mesh, and the pore size of the mesh is 0.05mm-10mm.
A kind of 9. device for producing bacteria cellulose composite material according to claim 1, it is characterised in that：The culture
Based containers (1) are closed container or open top type container.
A kind of 10. device for producing bacteria cellulose composite material according to claim 1, it is characterised in that：The bone
The linear velocity of frame material (13) transmission is 10~280m/h.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201720919990.8U CN207227839U (en) | 2017-07-27 | 2017-07-27 | A kind of device for producing bacteria cellulose composite material |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720919990.8U CN207227839U (en) | 2017-07-27 | 2017-07-27 | A kind of device for producing bacteria cellulose composite material |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107354746A (en) * | 2017-07-27 | 2017-11-17 | 东华大学 | A kind of device for producing bacteria cellulose composite material |
| EP3581705A1 (en) * | 2018-06-12 | 2019-12-18 | Sanko Tekstil Isletmeleri San. Ve Tic. A.S. | A process for providing a culture of microorganisms to an elongated element |
-
2017
- 2017-07-27 CN CN201720919990.8U patent/CN207227839U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107354746A (en) * | 2017-07-27 | 2017-11-17 | 东华大学 | A kind of device for producing bacteria cellulose composite material |
| EP3581705A1 (en) * | 2018-06-12 | 2019-12-18 | Sanko Tekstil Isletmeleri San. Ve Tic. A.S. | A process for providing a culture of microorganisms to an elongated element |
| US11485948B2 (en) | 2018-06-12 | 2022-11-01 | Sanko Tekstil Isletmeleri San. Ve Tic. A.S. | Process for providing a culture of microorganisms to an elongated element |
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