CN207198169U - A kind of agglutinating reaction external member for being used to detect pathogenic microorganism - Google Patents
A kind of agglutinating reaction external member for being used to detect pathogenic microorganism Download PDFInfo
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- CN207198169U CN207198169U CN201721146457.9U CN201721146457U CN207198169U CN 207198169 U CN207198169 U CN 207198169U CN 201721146457 U CN201721146457 U CN 201721146457U CN 207198169 U CN207198169 U CN 207198169U
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- external member
- agglutinating
- reactive tank
- agglutinating reaction
- reaction
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Abstract
The utility model discloses a kind of agglutinating reaction external member for being used to detect pathogenic microorganism.The agglutinating reaction external member includes agglutinating reaction plate and more than one mix rod；The agglutinating reaction plate includes transparent reaction plate, and the transparent reaction plate is provided with more than one reactive tank, and the bottom of the reactive tank is plane, and the inner surface of the reactive tank is coated with anti-protein adsorption layer；The mix rod surface is coated with the anti-protein adsorption layer.The utility model is applicable to various slide method agglutination tests, can be with disposable, direct sterilization treatment after use, not only mitigates operating personnel's workload, is more avoided that personnel, environment by detection sample pollution.
Description
Technical field
It the utility model is related to a kind of agglutinating reaction external member for being used to detect pathogenic microorganism.
Background technology
The serological test that aggegation occurs after being combined with corresponding antibodies for particulate antigen is referred to as agglutinating reaction experiment.Aggegation is anti-
The unknown antigen of available known detection of specific antibody (diagnosis or parting such as separation strain) should be tested, it is also possible to known
Antigen detects unknown antibody (such as rose bengal precipitation test).This method is with a long history, simple to operate, the reaction time is short, fits
For test in laboratory, Site Detection is also applied for.Wherein slide method is most widely used as qualitative test in basic unit.Glass
Piece method uses simple glass plate to be reused after being handled as reaction plate, the sterilized drying of glass plate etc..Needed before use according to sample
Product quantity and sample-adding amount draw square lattice with wax crayon on a glass, and grid is numbered；During use in grid
The antigen and antibody of ormal weight are added, by toothpick stirring or rocking glass plate makes both fully mix, small by there is aggegation
The degree of block carries out result judgement.The technology uses simple glass plate to be reused as reaction plate, glass plate, agglutinating reaction examination
The sample for testing detection is biological sample, and ability is reusable after sterilized, scrub, sterilizing, drying and processing are needed after glass plate use.
Needed before sample is detected with wax crayon on a glass frame and be numbered, not only lose time and the size of grid,
Shape can not realize standardization；Because glass plate is plane, the lines of crayon drawing do not have iris action, often can be because of liquid not
Controllable flowing causes cross pollution；Antigen-antibody is mixed frequently with the method for rocking glass plate in a large amount of sample detections, is
Sample cross contamination shaking amplitude very little is avoided, can not accomplish fully to mix influences result of the test；Glass plate is walked using post processing
It is rapid cumbersome, waste time and energy；Glass has the characteristics of frangible, quality is big in itself, and easily hurts sb.'s feelings, and is unfavorable for carrying with.
Agglutinating reaction experiment, which relies primarily on, is dissolved in liquid free state antigen-antibody reaction to realize, agglutinating reaction examination
Antigen and antibody in testing are protein, and protein can have necessarily in liquid solution during electrically charged contact solid reaction plate
The adsorption phenomena of ratio, influence agglutinating reaction result of the test.
Exploitation one kind can avoid because of antigen-antibody Adsorption Effect agglutinating reaction result and avoid occurring intersecting dirt between sample
Contaminate, be safe to use, the product for carrying out agglutinating reaction experiment that processing is simple, easy to carry has application value very much.
Utility model content
A technical problem to be solved in the utility model how is avoided because to influence aggegation anti-antigen-antibody adsorbs
Answer testing result and how to avoid cross pollution occur between sample.
To solve above technical problem, the utility model provides a kind of agglutinating reaction set for being used to detect pathogenic microorganism
Part.
It is provided by the utility model to be used to detect the agglutinating reaction external member of pathogenic microorganism, it include agglutinating reaction plate and
More than one mix rod；The agglutinating reaction plate includes transparent reaction plate, and the transparent reaction plate reacts provided with more than one
Groove, the bottom of the reactive tank is plane, and the inner surface of the reactive tank is coated with anti-protein adsorption layer；The mix rod surface
It is coated with the anti-protein adsorption layer.
In the above-mentioned agglutinating reaction external member for detecting pathogenic microorganism, the quantity of the mix rod depends on the reaction
The quantity of groove, a reactive tank can match somebody with somebody more than one described mix rod, as described in a reactive tank can match somebody with somebody 1 to 3
Mix rod.
In the above-mentioned agglutinating reaction external member for detecting pathogenic microorganism, the quantity of the reactive tank can be according to test sample to be checked
The quantity of product determines, as that can be provided with 1 to 96 reactive tank on the transparent reaction plate.
Above-mentioned to be used to detect in the agglutinating reaction external member of pathogenic microorganism, the reactive tank can be square groove, the reaction
The bottom of groove is the upper surface of the transparent reaction plate.
In the above-mentioned agglutinating reaction external member for detecting pathogenic microorganism, the cell wall height of the reactive tank can be 1.0-
3.5mm, the interior length and inner width of the reactive tank can be 2-4cm.
The above-mentioned agglutinating reaction external member for being used to detect pathogenic microorganism can be made up in addition to anti-protein adsorption layer of polystyrene.
Polystyrene is a kind of thermoplastic of water white transparency.
The anti-protein adsorption layer of the above-mentioned agglutinating reaction external member for detecting pathogenic microorganism is made up of polyvinyl alcohol.
In the above-mentioned agglutinating reaction external member for detecting pathogenic microorganism, the reactive tank is on the transparent reaction plate
Distribution can rectangular array.
In the above-mentioned agglutinating reaction external member for detecting pathogenic microorganism, the transparent reaction plate can be square plate, adjacent
Two edges be provided with and be used for mark zone that each reactive tank is numbered.
In the above-mentioned agglutinating reaction external member for detecting pathogenic microorganism, the transparent reaction plate is provided with Handheld Division.
In the above-mentioned agglutinating reaction external member for detecting pathogenic microorganism, the Handheld Division can have matte surface.
In the above-mentioned agglutinating reaction external member for detecting pathogenic microorganism, one end of the mix rod can be provided with handle.
The above-mentioned agglutinating reaction plate for being used to detect in the agglutinating reaction external member of pathogenic microorganism falls within of the present utility model
Protection domain.
The inner surface of reactive tank of the present utility model and the anti-protein adsorption layer on the mix rod surface, can be avoided because of antigen
Antibody adsorption reaction plate and influence agglutinating reaction testing result；Antigen-antibody is quick when mix rod is used for agglutinating reaction operation, fills
Divide and mix, one end is provided with handle, librarian use easy to operation；Antigenic solution can be added dropwise using square reactive tank, during use
In the side of most long-diagonal, the opposite side in most long-diagonal is added dropwise in antibody-solutions, before combination, antigen and antibody do not connect
Touch, can be after antigenic solution, the antibody-solutions of all samples of this plate be added dropwise when carrying out great amount of samples detection, then mix
The antigenic solution and antibody-solutions of each sample, make each sample reaction time try one's best it is synchronous, when being easy to the great amount of samples to detect
Unified timing, convenient operation.Transparent reaction plate is provided with handgrip part, easy to remove, shake up reaction plate, preferably completes experiment,
Handgrip part reaction plate can be marked when polylith reaction plate uses simultaneously after carrying out frosted processing；On transparent reaction plate
Provided with the high square reactive tanks of 1.0-3.5mm, the interior length of reactive tank and interior a width of 2-4cm, provided to different agglutinating reactions sufficient
Reaction compartment and avoid cross pollution occur between different samples；Two adjacent edges of transparent reaction plate, which are provided with, to be used for every
The mark zone that individual reactive tank is numbered can make the reactive tank of each sample have different numberings, save operating personnel and mark the time
Improve operating efficiency.The utility model is applicable to various slide method agglutination tests, can directly be gone out after use with disposable
Bacterium is handled, and not only mitigates operating personnel's workload, is more avoided that personnel, environment by detection sample pollution.
Brief description of the drawings
Fig. 1 is the structural representation of the agglutinating reaction plate of the agglutinating reaction external member for detecting pathogenic microorganism.
Fig. 2 is the structural representation of the mix rod of the agglutinating reaction external member for detecting pathogenic microorganism.
Fig. 3 is the diagrammatic cross-section of a reactive tank of the agglutinating reaction external member for detecting pathogenic microorganism.
Marked in figure as follows：1 represents transparent reaction plate, and 2 represent mix rod, and 3 represent reactive tank, and 4 represent anti-protein adsorption
Layer, 5 represent Handheld Division, and 6 represent mark zone.
Embodiment
The utility model is further described in detail with reference to embodiment, the embodiment provided is only
Illustrate the utility model, rather than in order to limit the scope of the utility model.
Material used, reagent etc., unless otherwise specified, are commercially obtained in following embodiments.
Embodiment 1, prepare agglutinating reaction external member for detecting pathogenic microorganism
The agglutinating reaction external member for being used to detect pathogenic microorganism of the present embodiment is in addition to anti-protein adsorption layer, by polystyrene
It is made, its structure is as Figure 1-3.The agglutinating reaction external member for being used to detect pathogenic microorganism includes transparent reaction plate 1 and mixed
Even rod 2, square reactive tank 3 is set on transparent reaction plate 1.The inner surface of reactive tank 3 is coated with anti-protein adsorption layer 4.Mix rod 2
Surface is coated with anti-protein adsorption layer 4.Distribution rectangular array of the reactive tank 3 on transparent reaction plate 1, rectangular array are 8 rows
12 row.The a height of 2mm of cell wall of reactive tank 3, interior a length of 3cm of reactive tank 3, interior a width of 2cm.The bottom of reactive tank 3 is transparent anti-
Answer the upper surface of plate 1.The agglutinating reaction external member for being used to detect pathogenic microorganism has 96 reactive tanks, 3,100 mix rods 2.
One end of mix rod 2 is provided with handle.Transparent reaction plate 1 is provided with Handheld Division 5, and the Handheld Division is handled by frosted.Transparent reaction plate 1
Adjacent two edges (long side and broadside) are provided with the mark zone 6 for being used for that each reactive tank to be numbered.Positioned at the mark of long side
Remember that area 6 numbers provided with alphabetical A-L, numbered positioned at the mark zone 6 of broadside provided with Arabic numerals 1-8.
Its preparation method is as follows：
First, thermoplastic shaping
Poured into a mould to obtain the transparent reaction plate 1 and mix rod 2 with 96 reactive tanks 3 with polystyrene.In transparent reaction plate 1
Two adjacent edges are provided for the mark zone 6 that each reactive tank is numbered.In transparent reaction plate 1, Handheld Division 5 is set,
The Handheld Division is handled by frosted.
2nd, anti-protein adsorption layer is prepared
1g polyvinyl alcohol is weighed, is added while stirring in 10L deionized waters, heated solution makes polyvinyl alcohol to 65-75 DEG C
Fully dissolving, obtains 0.1g/L poly-vinyl alcohol solutions.The closed preservation of room temperature.
Transparent reaction plate 1 and mix rod 2 are put into poly-vinyl alcohol solution and adsorbed 2 hours, then takes out transparent reaction plate 1
With mix rod 2 in 20-30 DEG C of drying, polyvinyl alcohol layer, the polyvinyl alcohol are formed in the inner surface of reactive tank 3 and the surface of mix rod 2
Layer is anti-protein adsorption layer 4.
3rd, pack
It is packaged together after prepare 100 mix rods 2 are sealed with one block of transparent reaction plate 1, obtains being used to detect
The agglutinating reaction external member of pathogenic microorganism, plastic bag sealing packaging.
Embodiment 2, the agglutinating reaction external member detection dysentery characterized by white mucous stool infected chicken for detecting pathogenic microorganism
Dysentery characterized by white mucous stool infected chicken whole blood flat board is carried out using the agglutinating reaction external member for being used to detect pathogenic microorganism of embodiment 1 to coagulate
Collection experiment, method are carried out with reference to fowl typhoid and white diarrhea diagnostic techniques (NY/T 536-2002), comprised the following steps that：
Under 20 DEG C~25 DEG C environmental conditions, antigen is drawn with pipettor, is respectively perpendicular and drips in 96 reactive tanks 3, often
0.05mL antigens are added dropwise in individual reactive tank 3, then take blood with the wing venous or hat point of 1 punctures, one dysentery characterized by white mucous stool infected chicken
0.05mL, wing venous or hat point that 96 syringe needles puncture 48 dysentery characterized by white mucous stool infected chickens and 48 healthy chickens respectively are shared, respectively takes blood
0.05mL, it is separately added into 96 reactive tanks 3, blood and antigen are sufficiently mixed uniformly by each reactive tank 3 with a mix rod 2,
And it is scattered to a diameter of 2cm, transparent reaction plate 1, timing result of determination are constantly shaken, while set positive serum, negative blood
Clear control.Experiment in triplicate, detects 48 dysentery characterized by white mucous stool infected chickens and 48 healthy chickens every time.
In 2min, positive serum is in 100% aggegation, and negative serum not aggegation, experiment is effectively.Tested whole blood goes out with antigen
Existing more than 50% aggegation person is the positive, and it is then feminine gender that aggegation does not occur.As a result show that positive serum is in 100% aggegation, negative blood
All there is more than 50% aggegation, all positives in clear not aggegation, 48 dysentery characterized by white mucous stool infected chickens, and 48 healthy chickens do not coagulate all
Collection, all feminine genders, illustrate that the agglutinating reaction external member detection dysentery characterized by white mucous stool for being used to detect pathogenic microorganism of the present invention infects accuracy rate
For 100%.
Claims (9)
- A kind of 1. agglutinating reaction external member, it is characterised in that：The agglutinating reaction external member includes agglutinating reaction plate and more than one is mixed Even rod；The agglutinating reaction plate includes transparent reaction plate, and the transparent reaction plate is provided with more than one reactive tank, the reaction The bottom of groove is plane, and the inner surface of the reactive tank is coated with anti-protein adsorption layer；The mix rod surface is coated with described Anti- protein adsorption layer.
- 2. agglutinating reaction external member according to claim 1, it is characterised in that：The reactive tank is square groove, the reaction The bottom of groove is the upper surface of the transparent reaction plate.
- 3. agglutinating reaction external member according to claim 2, it is characterised in that：The high 1.0-3.5mm of cell wall of the reactive tank, The interior length of the reactive tank and interior a width of 2-4cm.
- 4. agglutinating reaction external member according to claim 3, it is characterised in that：The agglutinating reaction external member is by polystyrene system Into.
- 5. agglutinating reaction external member according to claim 4, it is characterised in that：The reactive tank is on the transparent reaction plate Distribution rectangular array.
- 6. agglutinating reaction external member according to claim 5, it is characterised in that：The transparent reaction plate is square plate, adjacent Two edges be provided with and be used for mark zone that each reactive tank is numbered.
- 7. agglutinating reaction external member according to claim 6, it is characterised in that：The transparent reaction plate is provided with Handheld Division.
- 8. agglutinating reaction external member according to claim 7, it is characterised in that：The Handheld Division has matte surface.
- 9. agglutinating reaction external member according to claim 1, it is characterised in that：One end of the mix rod is provided with handle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721146457.9U CN207198169U (en) | 2017-09-08 | 2017-09-08 | A kind of agglutinating reaction external member for being used to detect pathogenic microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721146457.9U CN207198169U (en) | 2017-09-08 | 2017-09-08 | A kind of agglutinating reaction external member for being used to detect pathogenic microorganism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207198169U true CN207198169U (en) | 2018-04-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721146457.9U Expired - Fee Related CN207198169U (en) | 2017-09-08 | 2017-09-08 | A kind of agglutinating reaction external member for being used to detect pathogenic microorganism |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207198169U (en) |
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2017
- 2017-09-08 CN CN201721146457.9U patent/CN207198169U/en not_active Expired - Fee Related
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180406 Termination date: 20190908 |
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| CF01 | Termination of patent right due to non-payment of annual fee |