CN207169438U - A kind of equipment for separating liquid from solid - Google Patents
A kind of equipment for separating liquid from solid Download PDFInfo
- Publication number
- CN207169438U CN207169438U CN201721097284.6U CN201721097284U CN207169438U CN 207169438 U CN207169438 U CN 207169438U CN 201721097284 U CN201721097284 U CN 201721097284U CN 207169438 U CN207169438 U CN 207169438U
- Authority
- CN
- China
- Prior art keywords
- sliding block
- liquid
- equipment
- fixed seat
- solid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a kind of equipment for separating liquid from solid, it can also be used to the separation of cell and supernatant.The equipment for separating liquid from solid includes：Storage element；Filter element；Liquid collecting unit；And negative pressure generation unit；Filter element includes the first sliding block, the second sliding block provided with the second hole and the filter assemblies being arranged between the first sliding block and the second sliding block provided with the first hole, the filter assemblies cover the first hole and the second hole, and the first sliding block and the second sliding block can slide along the direction differed with solidliquid mixture filtering direction to be separated；Filter element is arranged between the storage element and liquid collecting unit；Negative pressure generation unit is tightly connected with liquid collecting unit.The equipment for separating liquid from solid and solid-liquid separating method can quick separating cell culture fluids by simple operations without centrifuge and extras, and because equipment for separating liquid from solid operation dimension is single, it can be passed directly in automation equipment, realize and the mass of sample is handled, enormously simplify the complexity of operation.
Description
Technical field
The utility model belongs to biomedicine field, is related to a kind of equipment for separating liquid from solid, it can also be used to cell and supernatant
Separation.
Background technology
, it is necessary to which first centrifugation could obtain the samples such as supernatant, nutrient solution, blood plasma in conventional cell culture experiment.Obtain
Clearly, especially the supernatant of cell culture, general use first are centrifuged again with the method on pipette transfer upper strata.The defects of such a method
It is：1st, centrifuge is needed；2nd, it can not automate, operation difficulty is added for cell culture.
The A of CN 102229888 disclose a kind of feedback-type pneumatic-control compression cell culture apparatus, the storage of described device mesohigh
Gas tank connects micro pressurization through switch, pressure-reducing valve, link conduit to three-way pipe a, and lower link conduit, link conduit, syringe
Pump, link conduit to three-way pipe b, and connecting conduit, pressure gauge and bacterial filter upper end, bacterial filter lower end connection syringe needle；Rubber
Plug is upper external reinforcement plate on cylinder type glass container, on rubber stopper, and lower external reinforcement plate is below cartridge type glass container, syringe needle
Penetrate rubber stopper to enter in cylinder type glass container, Tissue Culture Dish is placed in cylinder type glass container bottom.Which solves
The defects of closed container is neither easy to open, closed and can not bear a certain size compression.But the device can not be same
When for cell culture and the separation of solid and liquid of cell culture fluid.
And traditional filter is not particularly suited for the separation of solid and liquid of cell culture fluid, due to cell fragility, in traditional mistake
Direct draught is easy to rupture in filter, blocks filter membrane, can not separate supernatant haemocyte well.
Utility model content
In view of the deficienciess of the prior art, the purpose of this utility model is to provide a kind of equipment for separating liquid from solid, it is described
Equipment for separating liquid from solid without centrifuge and extras by simple operations can quick separating cell culture fluid, and by institute
It is single to state equipment for separating liquid from solid operation dimension, can be passed directly in automation equipment, realizes the mass processing to sample, greatly
The big complexity for simplifying operation.
To use following technical scheme up to this purpose, the utility model：
The utility model provides a kind of equipment for separating liquid from solid, and the equipment for separating liquid from solid includes：
Storage element, for storing solidliquid mixture to be separated；
Filter element, for filtering solidliquid mixture to be separated；
Liquid collecting unit, for collecting liquid caused by filter element；With,
Negative pressure generation unit, for making liquid collecting unit produce negative pressure；
The filter element includes provided with first sliding block in the first hole, the second sliding block provided with the second hole and is arranged at the
Filter assemblies between one sliding block and the second sliding block, the filter assemblies cover the first hole and the second hole, first sliding block
It can be slided with the second sliding block along the direction differed with solidliquid mixture filtering direction to be separated, i.e., described first sliding block and institute
The glide direction for stating the second sliding block can be any direction in addition to solidliquid mixture filtering direction (flow direction) to be separated, so
Set production, assembling and use all fairly simple；
The filter element is arranged between the storage element and liquid collecting unit；
The negative pressure generation unit is connected with liquid collecting unit, to cause liquid collecting unit to produce negative pressure.
The first sliding block and the second sliding block of the filter element can slide, so that storage element collects list with liquid
Member can be spaced from each other or be connected by filter assemblies, when needing filtering, be slided as long as sliding first sliding block and described second
Block causes filter assemblies to be placed on the pipeline that storage element connects with liquid collecting unit；When that need not filter, such as exist
Cell culture stage, slidably first sliding block and the second sliding block so that storage element is spaced from each other with liquid collecting unit.
Equipment for separating liquid from solid provided by the utility model can meet the separation of solid and liquid of cell culture and cell culture fluid simultaneously,
And the lock out operation complexity of the solidliquid mixtures such as cell culture fluid can be reduced.
First sliding block is provided with first raised, and second sliding block is provided with the first groove, described first it is raised and
First groove, which cooperates, is fixed on the filter assemblies between the first sliding block and the second sliding block.First projection and first
The setting of groove is to ensure the stability of filtering in order to avoid filter assemblies slide between the first sliding block and the second sliding block.
Preferably, the first sliding block both ends and the both ends of second sliding block are respectively arranged with the second projection, to prevent
First sliding block and the second sliding block slide between storage element and liquid collecting unit.
First sliding block and the second sliding block can slide along the direction vertical with solidliquid mixture filtering direction to be separated,
The direction is the moving direction of most convenient, and those skilled in the art can also set the first sliding block and the second sliding block according to being actually needed
Moving direction.
Preferably, the filter assemblies include filter membrane, and the filter membrane is selected from hydrophilic nylon film, microporous polypropylene membrane or poly- four
In fluoride microporous film any one or at least two combination, typical but non-limiting combination such as hydrophilic nylon film with it is poly-
Propylene microcellular film, hydrophilic nylon film and microporous teflon membran, hydrophilic nylon film, microporous polypropylene membrane and polytetrafluoroethylene (PTFE) are micro-
Pore membrane, preferably microporous teflon membran.Those skilled in the art can also select according to the property of solidliquid mixture to be separated
Different filter assemblies.
The storage element includes memotron and the first communicating pipe, and one end of first communicating pipe connects with memotron,
The other end of first communicating pipe is connected with filter element, and first communicating pipe is used for the solid-liquid to be separated in memotron
Mixture is input to filter element to filter, or preserves solidliquid mixture to be separated.
Preferably, the side of the memotron connects with the first communicating pipe.
Preferably, the connection end of first communicating pipe and filter element is provided with sealing ring, to prevent solid-liquid to be separated
Mixture is spilt.
Preferably, the storage element also includes the first fixed seat, the memotron and is fixed on the first communicating pipe described
In first fixed seat, in order to place.
The liquid collecting unit includes fluid collection tube and the second communicating pipe, one end of second communicating pipe and liquid
Collecting pipe connects, and the other end of second communicating pipe is connected with the filter element, and second communicating pipe is to by solid-liquid
Isolated liquid is input in fluid collection tube.
Preferably, the side of the fluid collection tube connects with the second communicating pipe.
Preferably, the connection end of second communicating pipe and filter element is provided with sealing ring, to prevent separation of solid and liquid from obtaining
To liquid spill.
Preferably, the liquid collecting unit also includes the second fixed seat, consolidates the fluid collection tube and the second communicating pipe
Due in second fixed seat, it is easy to the placement of liquid collecting unit.
The storage element sets at least two first communicating pipes, such as 2,3,4,5,6,7,8,9
Or 10 etc., the liquid collecting unit set at least two second communicating pipes, such as 2,3,4,5,6,7,8
Individual, 9 or 10 etc., the number of first communicating pipe is identical with the number of the second communicating pipe.Each first communicating pipe
It is connected by filter element with second communicating pipe, to be filtered when needing filtering to solidliquid mixture to be separated.
First fixed seat sets the 3rd raised and/or the 3rd groove, second fixed seat set the 4th groove and/
Or the 4th is raised, the storage element passes through the described 3rd raised and/or the 3rd groove and institute with the liquid collecting unit
State the 4th groove and/or the 4th raised the filter element is fixed between the first fixed seat and the second fixed seat.
First fixed seat and second fixed seat set positioning hole, first fixed seat and described second respectively
Fixed seat by the positioning hole and with positioning hole coordinate pilot pin by the filter element be fixed on the first fixed seat and
Between second fixed seat.
The negative pressure generation unit includes pull bar, and the pull bar is arranged in fluid collection tube, passes through push-and-pull push-and-pull
Bar so that negative pressure is produced in fluid collection tube.Those skilled in the art can also use other negative pressure generating devices, such as by liquid
Collecting pipe connects vavuum pump, and generation negative pressure is vacuumized using vavuum pump.
Preferably, the negative pressure generation unit also includes sealing ring, and the pull bar is provided with the second groove, the sealing
Circle is positioned in second groove, and the sealing ring can preferably produce negative pressure in fluid collection tube.
The utility model additionally provides a kind of solid-liquid separating method, and the solid-liquid separating method includes：Under condition of negative pressure,
Treat that separation of solid and liquid solidliquid mixture is filtered, obtain the liquid after separation of solid and liquid.
Compared with prior art, the beneficial effects of the utility model are：
Equipment for separating liquid from solid provided by the utility model can be quick by simple operations without centrifuge and extras
Dissociated cell culture liquid, traditional approach is more using centrifugation, minimum 5-10 minutes；The utility model was less than 1 minute, greatly
Traditional separate mode is simplified greatly；
Equipment for separating liquid from solid provided by the utility model can be passed directly to automation equipment because operation dimension is single
On, automation, the mass processing to sample are realized, this filter can be as needed, and different size is made, and tackles from 1mL
Cell conditioned medium to 100mL separates；Concatermer can be made, while handles multiple samples, is carried out in automation equipment at batch
Reason, can once handle 12-48 amount；
Equipment for separating liquid from solid provided by the utility model can be used for the separation of solid and liquid of cell culture and cell culture fluid simultaneously.
Brief description of the drawings
Fig. 1 is the structural representation for the equipment for separating liquid from solid that embodiment 1 provides.
Fig. 2 is the structural representation in A directions in Fig. 1 that embodiment 1 provides.
Fig. 3 is the structural representation in B directions in Fig. 1 that embodiment 1 provides.
Fig. 4 is the structural representation for the storage element that embodiment 1 provides.
Fig. 5 is the structural representation for the first sliding block that embodiment 1 provides.
Fig. 6 is the structural representation for the second sliding block that embodiment 1 provides.
Fig. 7 is the structural representation for the piston that embodiment 1 provides.
Fig. 8 is the structural representation for the pilot pin that embodiment 1 provides.
Wherein：1, memotron；2, positioning hole；3, pull bar；4, sliding shutter；5, fluid collection tube；6, the first sealing ring；
7, the first communicating pipe；8, the 3rd groove；9, pilot pin；10, the first sliding block；11, first is raised；12, the second sliding block；13, first
Groove；14, the 3rd is raised；15, second is raised；16, the first fixed seat；17, the second fixed seat；18, the second sealing ring.
Embodiment
Further illustrate the technical solution of the utility model below in conjunction with the accompanying drawings and by embodiment.
Embodiment 1
A kind of equipment for separating liquid from solid, as Figure 1-3, the equipment for separating liquid from solid include：
Storage element, for storing solidliquid mixture to be separated；
Filter element, for filtering solidliquid mixture to be separated；
Liquid collecting unit, for collecting liquid caused by filter element；With,
Negative pressure generation unit, for making liquid collecting unit produce negative pressure；
The filter element includes the first sliding block 10 (as shown in Figure 5) provided with the first hole, the second cunning provided with the second hole
Block 12 (as shown in Figure 6) (sliding block 12 of the first sliding block 10 and second form sliding shutter 4) and it is arranged at the first sliding block 10
And the second filter assemblies between sliding block 12, the filter assemblies cover the first hole and the second hole, the He of the first sliding block 10
Second sliding block 12 can slide along the direction vertical with solidliquid mixture filtering direction to be separated；The filter element is arranged at institute
State between storage element and liquid collecting unit；The negative pressure generation unit is tightly connected with liquid collecting unit；
First sliding block 10 and second sliding block 12 also can be along in addition to vertical direction and solidliquid mixtures to be separated
The direction that filtering direction differs is slided, as long as can ensure to need filtering, the solidliquid mixture to be separated in storage element
Can filtered component filters, filtrate enters liquid collecting unit；When need not filter, the storage element is collected single with liquid
Do not connected between member；
First sliding block 10 is provided with the first projection 11, and second sliding block 12 is provided with the first groove 13, and described
The filter assemblies are fixed between the first sliding block 10 and the second sliding block 12 by one projection 11 and the first groove 13；
The both ends of first sliding block 10 and the both ends of second sliding block 12 are respectively arranged with the second projection 15, to prevent on-slip
Block slides from storage element and liquid collecting unit；
The filter assemblies include filter membrane, and the filter membrane is selected from hydrophilic nylon film, microporous polypropylene membrane or polytetrafluoroethylene (PTFE)
In microporous barrier any one or at least two combination, preferably microporous teflon membran；
The storage element is as shown in figure 4, including memotron 1, the first communicating pipe 7 and the first fixed seat 16, the storage
The communicating pipe 7 of pipe 1 and first is fixed in first fixed seat 16, one end of first communicating pipe 7 and the side of memotron 1
Connection, the other end connection filter element of first communicating pipe 7；First communicating pipe 7 and the connection of the filter element
End is provided with the first sealing ring 6；
The liquid collecting unit includes fluid collection tube 5, the second communicating pipe and the second fixed seat 17, and the liquid is collected
The communicating pipe of pipe 5 and second is fixed in second fixed seat 17, one end of second communicating pipe and the one of fluid collection tube 5
Side is connected, the other end connection filter element of second communicating pipe, and the connection end of second communicating pipe and filter element is set
It is equipped with sealing ring；
Further, the storage element set at least two first communicating pipes 7, such as 2,3,4,5,6,7
Individual, 8,9 or 10 etc., the liquid collecting unit set at least two second communicating pipes, such as 2,3,4,5,6
Individual, 7,8,9 or 10 etc., the number of first communicating pipe 7 is identical with the number of the second communicating pipe, and each first connects
Siphunculus 7 is connected by filter assemblies with the second communicating pipe, in order to which the equipment for separating liquid from solid mass handles solidliquid mixture；
The negative pressure generation unit is piston, as shown in fig. 7, comprises the sealing ring 18 of pull bar 3 and second, the pull bar
3 are provided with the second groove, and second sealing ring 18 is positioned in second groove, described to carry pushing away for the second sealing ring 18
Pull bar 3 is placed in the fluid collection tube 5, and is tightly connected with the fluid collection tube 5, and the pull bar 3 can be in the liquid
Slided in body collecting pipe 5, so as to produce negative pressure in the fluid collection tube 5；
The side of first fixed seat 16 is provided with the 3rd projection 14, and opposite side is provided with the 3rd groove 8, and described second is solid
The side of reservation 17 is provided with the 4th groove, and opposite side is provided with the 4th projection, positioning hole is set respectively on the raised and groove,
The storage element and the liquid collecting unit by it is described it is raised be connected with groove, and by the positioning hole 2 with
And with the fixation (as shown in Figure 8) of pilot pin 9 that positioning hole 2 coordinates, the filter element is fixed on the first fixed seat 16 and the
Between two fixed seats 17.
Solid-liquid separating method using the equipment for separating liquid from solid is：Under condition of negative pressure, treat that separation of solid and liquid solid-liquid mixes
Thing is filtered, obtains the liquid after separation of solid and liquid, specifically, comprises the following steps：
(1) solidliquid mixture to be separated is placed in memotron 1；
(2) filter element is slided, the filter assemblies of the filter element are placed in and connected with second the first communicating pipe 7
On the pipeline of pipe connection；
(3) sliding the pull bar 3 makes to produce negative pressure in fluid collection tube 5, the filtered component of solidliquid mixture to be separated
Enter fluid collection tube 5 after filtering, the liquid after separation of solid and liquid is obtained in fluid collection tube 5.
The solidliquid mixture to be separated can be the samples such as cell culture fluid, blood plasma.
50mL cell culture fluids, required time 50s are separated using the equipment for separating liquid from solid described in embodiment 1, and is used
Traditional centrifugation is, it is necessary to which 10min is acted on.
In addition, the memotron 1 of equipment for separating liquid from solid described in embodiment 1 and the volume of fluid collection tube 5 can be more than 100mL,
For samples such as first separation 100mL cell culture fluids or blood plasma.
Applicant states, the foregoing is only specific embodiment of the present utility model, but protection model of the present utility model
Enclose and be not limited thereto, person of ordinary skill in the field is it will be clearly understood that any belong to those skilled in the art and exist
In the technical scope that the utility model discloses, the change or replacement that can readily occur in all fall within the scope of protection of the utility model
Within the scope of disclosure.
Claims (10)
1. a kind of equipment for separating liquid from solid, it is characterised in that the equipment for separating liquid from solid includes：
Storage element, for storing solidliquid mixture to be separated；
Filter element, for filtering solidliquid mixture to be separated；
Liquid collecting unit, for collecting liquid caused by filter element；With,
Negative pressure generation unit, for making liquid collecting unit produce negative pressure；
The filter element includes the first sliding block provided with the first hole, the second sliding block provided with the second hole and is arranged at the first cunning
Filter assemblies between block and the second sliding block, the filter assemblies cover the first hole and the second hole, first sliding block and the
Two sliding blocks can slide along the direction differed with solidliquid mixture filtering direction to be separated；
The filter element is arranged between the storage element and liquid collecting unit；
The negative pressure generation unit is tightly connected with liquid collecting unit.
2. equipment for separating liquid from solid according to claim 1, it is characterised in that first sliding block is provided with the first projection,
Second sliding block is provided with the first groove, and the filter assemblies are fixed on the first sliding block by first projection and the first groove
And second between sliding block.
3. equipment for separating liquid from solid according to claim 1, it is characterised in that first sliding block and the second sliding block being capable of edges
Slide in the direction vertical with solidliquid mixture filtering direction to be separated.
4. equipment for separating liquid from solid according to claim 1, it is characterised in that the filter assemblies include filter membrane, the filter
Any one of film in hydrophilic nylon film, microporous polypropylene membrane or microporous teflon membran.
5. equipment for separating liquid from solid according to claim 1, it is characterised in that the storage element includes memotron, first
Communicating pipe and the first fixed seat, one end of first communicating pipe connect with memotron, the other end of first communicating pipe with
Filter element connects, and the memotron and is fixed on the first communicating pipe in first fixed seat.
6. equipment for separating liquid from solid according to claim 5, it is characterised in that the liquid collecting unit is collected including liquid
Pipe, the second communicating pipe and the second fixed seat, one end of second communicating pipe connect with fluid collection tube, second communicating pipe
The other end be connected with filter element, the fluid collection tube and be fixed on the second communicating pipe in second fixed seat.
7. equipment for separating liquid from solid according to claim 6, it is characterised in that the storage element sets at least two first
Communicating pipe, the liquid collecting unit set at least two second communicating pipes, and the number of first communicating pipe connects with second
The number of pipe is identical.
8. equipment for separating liquid from solid according to claim 6, it is characterised in that first fixed seat sets the 3rd projection
And/or the 3rd groove, second fixed seat set the 4th groove and/or the 4th raised, the storage element and the liquid
Collector unit is raised by the filtering by the described 3rd raised and/or the 3rd groove and the 4th groove and/or the 4th
Unit is fixed between the first fixed seat and the second fixed seat.
9. equipment for separating liquid from solid according to claim 6, it is characterised in that first fixed seat and described second is fixed
Seat sets positioning hole, first fixed seat and second fixed seat to coordinate by the positioning hole and with positioning hole respectively
Pilot pin the filter element is fixed between the first fixed seat and the second fixed seat.
10. equipment for separating liquid from solid according to claim 1, it is characterised in that the negative pressure generation unit includes pull bar
And sealing ring, the second groove is provided with the pull bar, and the sealing ring is positioned in second groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721097284.6U CN207169438U (en) | 2017-08-28 | 2017-08-28 | A kind of equipment for separating liquid from solid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721097284.6U CN207169438U (en) | 2017-08-28 | 2017-08-28 | A kind of equipment for separating liquid from solid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207169438U true CN207169438U (en) | 2018-04-03 |
Family
ID=61738419
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721097284.6U Active CN207169438U (en) | 2017-08-28 | 2017-08-28 | A kind of equipment for separating liquid from solid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207169438U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107441939A (en) * | 2017-08-28 | 2017-12-08 | 广州市雷德生物科技有限公司 | A kind of equipment for separating liquid from solid and solid-liquid separating method |
-
2017
- 2017-08-28 CN CN201721097284.6U patent/CN207169438U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107441939A (en) * | 2017-08-28 | 2017-12-08 | 广州市雷德生物科技有限公司 | A kind of equipment for separating liquid from solid and solid-liquid separating method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104272109B (en) | Sample introduction system | |
| DK2361301T3 (en) | DEVELOPMENT AND METHODS OF TREATING BIOLOGICAL MATERIAL | |
| US10675628B2 (en) | Fluid transfer device and process of aseptically transferring a fluid | |
| EP3041594B1 (en) | Filter device for filtering complex fluid samples | |
| CN204008653U (en) | Blood sampling transmission equipment, blood separation and test macro and blood sampling transmission system | |
| CN203899243U (en) | Synchronous suction filtration device for different grain diameters by layers | |
| US20110000324A1 (en) | Method and apparatus for sample processing and injection | |
| CN207169438U (en) | A kind of equipment for separating liquid from solid | |
| CN207877718U (en) | The micro-fluidic sampling device of bubble-free of cell solution | |
| WO2013043109A2 (en) | Device and method for processing a patient sample | |
| CN202397870U (en) | Blood separating device | |
| CN105816928A (en) | Blood composition acquisition device and method | |
| CN107441939A (en) | A kind of equipment for separating liquid from solid and solid-liquid separating method | |
| CN208260308U (en) | A kind of multistage Suction filtration device | |
| CN206933216U (en) | A kind of filter plant of rose enzyme beverage | |
| CN104614538A (en) | Multifunctional disposable transfusion and cross blood-matching test combiner | |
| CN214597504U (en) | Sediment gap water filtering device | |
| CN204422550U (en) | Multiplex disposable blood transfusion cross match blood test combiner | |
| CN112322468A (en) | Unidirectional negative pressure adsorption sampling and filtering device and method | |
| CN212504936U (en) | Device for preparing immune cells in blood on large scale | |
| CN112452020A (en) | Multi-connection solid-liquid separation vacuum filtration system | |
| CN208574485U (en) | A kind of graded combination Suction filtration device | |
| CN102295259B (en) | High-pressure fluid injection apparatus with automatic bubble discharging function | |
| CN214182023U (en) | Multi-connection solid-liquid separation vacuum filtration system | |
| CN216934843U (en) | Funnel and psammitolite suction filter device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |