CN207147848U - A kind of biological sample controllable temperature immediate dialysis device - Google Patents
A kind of biological sample controllable temperature immediate dialysis device Download PDFInfo
- Publication number
- CN207147848U CN207147848U CN201721131585.6U CN201721131585U CN207147848U CN 207147848 U CN207147848 U CN 207147848U CN 201721131585 U CN201721131585 U CN 201721131585U CN 207147848 U CN207147848 U CN 207147848U
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- buffer solution
- low
- cup
- water
- temperature
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Abstract
The utility model discloses a kind of biological sample controllable temperature immediate dialysis device,It is related to dialysis apparatus,Cylindrical jacket is provided with the outside of cylindrical cup,Cylindrical cup is integrally formed with cylindrical jacket,The two is an entirety,Top and bottom connect together completely,Centre is water bath with thermostatic control layer,Screw lid is provided with the top of cylindrical cup,Buffer solution Effluent criteria female Luer is provided with the upper and lower part side wall of cylindrical jacket and cylindrical cup,Buffer solution water inlet standard Luer interface,Low-temperature circulating water Effluent criteria female Luer,Low-temperature circulating water water inlet standard Luer interface,The water outlet joint on buffer solution cup top is connected to form a buffer solution circulation loop by peristaltic pump and buffer solution inlet channel with buffer solution water inlet standard Luer interface,Dialysis time can be greatly shortened in this device,Save elution buffer；Its temperature control does not need expensive chromatography freezer, and low temperature dialysis can be also carried out under conditions of no low-temperature circulating water bath equipment.
Description
Technical field
Dialysis apparatus is the utility model is related to, specially a kind of biological sample controllable temperature immediate dialysis device.
Background technology
Dialysis is a kind of conventional method in biological sample processing procedure, is usually used in the replacing of buffer solution, also can be used for weight
The renaturation of histone.But conventional dialysis is a time-consuming poorly efficient process, is usually put into the bag filter equipped with sample solution
In the large beaker for filling dialyzate, beaker is placed on magnetic stirring apparatus, and beaker bottom is placed with magnetic stir bar, beaker top uncovered
Or covered with culture dish.Whole dialysis apparatus is placed in chromatography freezer 2-3 days are dialysed under low temperature even for more time.It is not
It is to need special Cryo Equipment chromatography freezer, it is necessary to repeatedly change buffer solution in place of foot, elution buffer dosage is big, dialysis
Overlong time, protein and other is degradable or is denatured.Need to change repairing for the large biological molecule of buffer solution to some
It is more totally unfavorable for decorations reaction, because modification intermediate product is unstable in aqueous buffer solution, it is necessary in the most short time
The interior replacing for completing elution buffer.
The content of the invention
The purpose of this utility model is to provide a kind of biological sample controllable temperature immediate dialysis device, to solve above-mentioned background
The problem of technology is present.
To achieve the above object, the utility model provides following technical scheme：It include screw lid 1, cylindrical jacket 2,
Cylindrical cup 3, peristaltic pump 4, attemperater 5, thermometer 6, water bath with thermostatic control layer 7, the first low-temperature circulating waterpipe 8-1, second
Low-temperature circulating waterpipe 8-2, buffer solution outlet conduit 9-1, buffer solution inlet channel 9-2, buffer solution cup a and low temperature cup b, circle
The outside of cylindricality cup 3 is provided with cylindrical jacket 2, and cylindrical cup 3 is integrally formed with cylindrical jacket 2, and the two is
One entirety, top and bottom connect together completely, and centre is water bath with thermostatic control layer 7, and the top of cylindrical cup 3 is provided with
Buffer solution Effluent criteria Rule is provided with the upper and lower part side wall of screw lid 1, cylindrical jacket 2 and cylindrical cup 3 to connect
Mouth 1-1, buffer solution water inlet standard Luer interface 1-4, low-temperature circulating water Effluent criteria female Luer 1-3, low-temperature circulating water water inlet
Standard Luer interface 1-2, and buffer solution Effluent criteria female Luer 1-1 and buffer solution water inlet standard Luer interface 1-4 and cylinder
Shape cup 3 connects, low-temperature circulating water water inlet standard Luer interface 1-2 and low-temperature circulating water Effluent criteria female Luer 1-3 and perseverance
Tepidarium layer 7 connects, and buffer solution Effluent criteria female Luer 1-1 passes through buffer solution outlet conduit 9-1 and buffer solution cup a tops
Water inlet connection connects, and the water outlet joint on buffer solution cup a tops passes through peristaltic pump 4 and buffer solution inlet channel 9-2 and buffering
Liquid water inlet standard Luer interface 1-4 connects to form a buffer solution circulation loop, low-temperature circulating water Effluent criteria female Luer 1-3
It is connected by the first low-temperature circulating waterpipe 8-1 with the water inlet connection on low temperature cup b tops, the water outlet on low temperature cup b tops
Mouth joint connects shape by the low-temperature circulating waterpipe 8-2 of peristaltic pump 4 and second with low-temperature circulating water water inlet standard Luer interface 1-2
Into a low temperature water-flow circuit, and buffer solution cup a and low temperature cup b are arranged on inside attemperater 5.
Preferably, the described bag filter for being filled with sample liquid or dialysis tubing are placed in the cylinder full of elution buffer
In cup 3, you can easily carry out temperature control immediate dialysis.Dialysis cup is designed as different size specification, can be with not co-content
Sample liquid matching to be dialysed.
Preferably, described attemperater 5 is internally provided with thermometer 6.
Preferably, water built in described water bath with thermostatic control layer 7 and low temperature ice bag, controlling water temperature is between 0-10 DEG C.
Compared with prior art, the beneficial effects of the utility model are：The present apparatus, can be significantly compared with conventional dialysis mode
Degree shortens dialysis time, and several hours are shorten to from a couple of days；Compared with conventional dialysis mode, elution buffer can be significantly saved；
Compared with conventional dialysis mode, its temperature control does not need expensive chromatography freezer, or even the bar in no low-temperature circulating water bath equipment
Also low temperature dialysis can be carried out under part.
Brief description of the drawings
Fig. 1 is the utility model structure schematic elevation view.
In figure：It includes screw lid 1, cylindrical jacket 2, cylindrical cup 3, peristaltic pump 4, attemperater 5, thermometer
6th, water bath with thermostatic control layer 7, the first low-temperature circulating waterpipe 8-1, the second low-temperature circulating waterpipe 8-2, buffer solution outlet conduit 9-1,
Buffer solution inlet channel 9-2, buffer solution cup a and low temperature cup b.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belong to the scope of the utility model protection.
Referring to Fig. 1, the utility model provides a kind of technical scheme：It includes screw lid 1, cylindrical jacket 2, cylinder
Cup 3, peristaltic pump 4, attemperater 5, thermometer 6, water bath with thermostatic control layer 7, the first low-temperature circulating waterpipe 8-1, the second low temperature follow
Ring waterpipe 8-2, buffer solution outlet conduit 9-1, buffer solution inlet channel 9-2, buffer solution cup a and low temperature cup b, cylindrical cup
The outside of son 3 is provided with cylindrical jacket 2, and cylindrical cup 3 is integrally formed with cylindrical jacket 2, and the two is one whole
Body, top and bottom connect together completely, and centre is water bath with thermostatic control layer 7, and the top of cylindrical cup 3 is provided with screw lid
1, be provided with the upper and lower part side wall of cylindrical jacket 2 and cylindrical cup 3 buffer solution Effluent criteria female Luer 1-1,
Buffer solution water inlet standard Luer interface 1-4, low-temperature circulating water Effluent criteria female Luer 1-3, low-temperature circulating water enter water quality standard Shandong
You are interface 1-2, and buffer solution Effluent criteria female Luer 1-1 and buffer solution water inlet standard Luer interface 1-4 and cylindrical cup 3
Connection, low-temperature circulating water water inlet standard Luer interface 1-2 and low-temperature circulating water Effluent criteria female Luer 1-3 and water bath with thermostatic control layer
7 connections, buffer solution Effluent criteria female Luer 1-1 are connect by the water inlet on buffer solution outlet conduit 9-1 and buffer solution cup a tops
Head connection, the water outlet joint on buffer solution cup a tops enter water gauge by peristaltic pump 4 and buffer solution inlet channel 9-2 with buffer solution
Quasi- female Luer 1-4 connects to form a buffer solution circulation loop, and low-temperature circulating water Effluent criteria female Luer 1-3 passes through first
Low-temperature circulating waterpipe 8-1 is connected with the water inlet connection on low temperature cup b tops, and the water outlet joint on low temperature cup b tops is led to
Cross the low-temperature circulating waterpipe 8-2 of peristaltic pump 4 and second and low-temperature circulating water water inlet standard Luer interface 1-2 connect to be formed one it is low
Warm water circulation loop, and buffer solution cup a and low temperature cup b are arranged on inside attemperater 5.
Preferably, the described bag filter for being filled with sample liquid or dialysis tubing are placed in the cylinder full of elution buffer
In cup 3, you can easily carry out temperature control immediate dialysis.Dialysis cup is designed as different size specification, can be with not co-content
Sample liquid matching to be dialysed.
Preferably, described attemperater 5 is internally provided with thermometer 6.
Preferably, water built in described water bath with thermostatic control layer 7 and low temperature ice bag, controlling water temperature is between 0-10 DEG C.
While there has been shown and described that embodiment of the present utility model, for the ordinary skill in the art,
It is appreciated that these embodiments can be carried out with a variety of changes in the case where not departing from principle of the present utility model and spirit, repaiied
Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.
Claims (3)
- A kind of 1. biological sample controllable temperature immediate dialysis device, it is characterised in that：It includes screw lid (1), cylindrical jacket (2), cylindrical cup (3), peristaltic pump (4), attemperater (5), thermometer (6), water bath with thermostatic control layer (7), the first low-temperature circulating Waterpipe (8-1), the second low-temperature circulating waterpipe (8-2), buffer solution outlet conduit (9-1), buffer solution inlet channel (9-2), Buffer solution cup (a) and low temperature cup (b), cylindrical jacket (2), cylindrical cup (3) are provided with the outside of cylindrical cup (3) It is integrally formed with cylindrical jacket (2), the two is an entirety, and top and bottom connect together completely, and centre is Water bath with thermostatic control layer (7), screw lid (1), cylindrical jacket (2) and cylindrical cup (3) are provided with the top of cylindrical cup (3) Upper and lower part side wall on be provided with buffer solution Effluent criteria female Luer (1-1), buffer solution water inlet standard Luer interface (1- 4), low-temperature circulating water Effluent criteria female Luer (1-3), low-temperature circulating water water inlet standard Luer interface (1-2), and buffer solution goes out Water quality standard female Luer (1-1) connects with buffer solution water inlet standard Luer interface (1-4) with cylindrical cup (3), low-temperature circulating Water water inlet standard Luer interface (1-2) and low-temperature circulating water Effluent criteria female Luer (1-3) connect with water bath with thermostatic control layer (7), Buffer solution Effluent criteria female Luer (1-1) is connect by buffer solution outlet conduit (9-1) and the water inlet on buffer solution cup (a) top Head connection, the water outlet joint on buffer solution cup (a) top pass through peristaltic pump (4) and buffer solution inlet channel (9-2) and buffer solution Standard Luer interface (1-4) connection of intaking forms a buffer solution circulation loop, low-temperature circulating water Effluent criteria female Luer (1- 3) it is connected by the first low-temperature circulating waterpipe (8-1) with the water inlet connection on low temperature cup (b) top, on low temperature cup (b) The water outlet joint in portion passes through peristaltic pump (4) and the second low-temperature circulating waterpipe (8-2) and low-temperature circulating water water inlet standard Luer Interface (1-2) connection forms a low temperature water-flow circuit, and buffer solution cup (a) and low temperature cup (b) are arranged on attemperater (5) it is internal.
- A kind of 2. biological sample controllable temperature immediate dialysis device according to claim 1, it is characterised in that：Described guarantor Reservoir (5) is internally provided with thermometer (6).
- A kind of 3. biological sample controllable temperature immediate dialysis device according to claim 1, it is characterised in that：Described constant temperature Water built in water-bath layer (7) and low temperature ice bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721131585.6U CN207147848U (en) | 2017-09-05 | 2017-09-05 | A kind of biological sample controllable temperature immediate dialysis device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721131585.6U CN207147848U (en) | 2017-09-05 | 2017-09-05 | A kind of biological sample controllable temperature immediate dialysis device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207147848U true CN207147848U (en) | 2018-03-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721131585.6U Expired - Fee Related CN207147848U (en) | 2017-09-05 | 2017-09-05 | A kind of biological sample controllable temperature immediate dialysis device |
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| CN (1) | CN207147848U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111659482A (en) * | 2020-06-11 | 2020-09-15 | 南昌航空大学 | Device for quickly and accurately controlling temperature of quartz microbalance flow cell and construction method thereof |
-
2017
- 2017-09-05 CN CN201721131585.6U patent/CN207147848U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111659482A (en) * | 2020-06-11 | 2020-09-15 | 南昌航空大学 | Device for quickly and accurately controlling temperature of quartz microbalance flow cell and construction method thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180327 Termination date: 20200905 |