CN207123535U - A kind of chemiluminescence immunoassay kit of Quantitative detection cardiac troponin - Google Patents
A kind of chemiluminescence immunoassay kit of Quantitative detection cardiac troponin Download PDFInfo
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- CN207123535U CN207123535U CN201721215016.XU CN201721215016U CN207123535U CN 207123535 U CN207123535 U CN 207123535U CN 201721215016 U CN201721215016 U CN 201721215016U CN 207123535 U CN207123535 U CN 207123535U
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Abstract
It the utility model is related to a kind of chemiluminescence immunoassay kit of Quantitative detection cardiac troponin, kit includes detection and blocked, it is luminous to start reagent and chemical illumination immunity analysis instrument, detection card includes loading pad, chemiluminescence pad, carrier film with detection line and nature controlling line, sample suction pad, bottom plate and boosting pad, chemiluminescence pad is included with reference to pad body and coated in the chemical illuminating reagent labelled antibody coating combined on pad body, antibody in chemical illuminating reagent labelled antibody coating is the antibody of cardiac troponin, detection line is the coating for the pairing antibody or antigen formation that can be combined with the antibody specificity of cardiac troponin.The utility model can have the advantages that easy to operate, rapid reaction, sensitivity height, high specificity, for acute myocardial infarction early warning and state of an illness risk judgment with Quantitative detection cardiac troponin.
Description
Technical field
It the utility model is related to biotechnology diagnostic field, and in particular to a kind of Quantitative detection cardiac troponin
Chemiluminescence immunoassay kit.
Background technology
Acute myocardial infarction AMI (acute myocardial infarction, AMI) is the Severe acute disease of cardiovascular system,
The incidence of disease is high, and prognosis is poor, and the death rate is high.For without typical chest pain and the unconspicuous myocardial infarction patient of ECG change, only
By electrocardiogram, echocardiogram and heart nuclear magnetic resonance, it is difficult to Accurate Diagnosis.And traditional detection project is mostly myocardium enzyme
Spectrum, but enzymatic activity rise be present and the deficiencies of later, the specificity poor and duration is short occur.Cardiac troponin is uniquely to deposit
It is the contractile protein of cardiac muscle, has high susceptibility and specificity to myocardial necrosis.
One of three subunits (cTnI, cTnC and cTnT) for cardiac troponin (cTn), are contraction of muscle tissue
Regulatory protein, it is polypeptide in itself, on the actin filament of contractile protein, important tune is played during contraction of muscle and diastole
Section acts on.It has unique sequence, molecular weight 24000 on gene.The TnI of different tissue sources is special in a organized way
Property and without species specificity.CTnI is existed only in heart muscle and ventricular muscles, and a kind of only TnI is present, fetus, neonate and
The cTnI all sames in each stages such as adult.So cTnI is a kind of preferably cardiac myocytespecific mark.
In the case of myocardial cell membrane is complete, cTnI can not appear cell membrane and enter blood circulation.Cardiac muscle cells are because lacking
When degeneration necrosis occurs for blood, anoxic, cTnI is released into blood by damaged cell membrane.Because cTnI molecular weight is smaller and continues from change
Property escape into the cell, therefore after AMI occurs, occur in blood relatively early, and continue for quite a long time.Compared with other sero-enzymes,
CTnI is the more special label of myocardial damage, while it is also very sensitive, can be performed the operation with Diagnosing Cardiac（Such as：Calcified master
Arterial valve is narrow and coronary bypass）There is 4h AMI in the small infarct and ischemic of surrounding.Compared with CTnI, cTnC
Without Cardiac-specific, myocardial damage detection is generally not used for.CTnI and cTnT is unable to the entrance of penetration cell film under normal condition
Blood, so cTnI and cTnT are extremely low in Healthy People blood；Such as myocardial cell damage, cTnI and cTnT enter people's cell interstitial and blood
Liquid.In the diseases such as kidney failure, pneumonia and septicemia, cTnT contents can also raise in blood, so its specificity is not so good as cTnI.
Content is very low in normal human blood, about 20.4pg/ml.cTnI >20.4pg/ml, myocardial damage is just examined
Disconnected meaning.Patient can be detected with cTnI there is the minor myocardial damage of pectoralgia symptom a few days ago.Occur in AMI
2.2-6.8 hours, its concentration start to raise, and about reach peak value 195.9ng/ml at 11.2 hours, and the duration is long, 5~
It can be down to after 10 days normal.CTnI dynamic releases after myocardial damage are rapid, and curve is complete, and peak value is obvious, window diagnostic period length,
Compared with current diagnosis miocardial infarction (MI) index, cTnI has advantages below：Time of occurrence is early in blood because molecular weight compared with
CK-MB is small, is more easy to be released into blood after myocardial damage；High sensitivity；Specific high, Skeletal muscle injury does not influence the judgement of result,
Also it can speculate that the type of surgery of non-cardiac surgery patient has no significant effect to cTnI；Duration is grown, therefore can be in myocardial damage
Early stage and later stage clarify a diagnosis by detecting cTnI and judge Myocardial injury degree.In acute myocardial infarction diagnosis, treatment and prognosis
With important clinical value.
At present, cardiac troponin（CTnI）Detection method mainly have radioimmunology (RIA), enzyme immunoassay
(EIA), colloidal gold immunity chromatography, microfluidic method, fluorescence immune chromatography method etc..Radioimmunology (RIA) method measure takes time
Longer (2 days), detection sensitivity are relatively low (10 μ g/L), are not suitable for AMI early diagnosis and the diagnosis of small infarct.Enzyme is exempted from
Epidemic disease determination method (EIA) method minute is 3.5h, and complex operation, the testing time is long, detection range is narrow, for the diagnosis to AMI
Still dislike time-consuming long.Colloidal gold immunity chromatography qualitatively or quantitatively detects, and sensitivity is low, the range of linearity is narrow, it is impossible to meet it is quantitative,
The requirement accurately detected；Microfluidic method can quantify, accurately while detect cardiac muscle three, but generally require more sample size,
And reagent cost is high；Most of fluorescence immune chromatography method uses fluorescein or other materials as light emitting source, although having higher
The range of linearity, but disturbed by background signal, it is impossible to ensure low side sensitivity.
Because CTnI clinical medicine determines that level is very low, medically it is badly in need of a kind of sensitive, accurate, economic, easy inspection
Survey method detects to it, and chemiluminescence has highest sensitivity in all detection method at present, this research be by
The solid phase labelling immunoassay technology that chemiluminescence immunoassay technology and immunochromatography technique organically combine, Gao Ling
The light reaction of sensitivity is combined with specific immunological response, and the degree of accuracy is high, precision is good, in combination with immunochromatography skill
The advantages of art is quick, economic, obviates traditional chemical and light that time-consuming for Enzyme-multiplied immune technique, the defects of poor repeatability.Can be fast
Speed, accurately it is used for acute myocardial infarction early warning and state of an illness risk judgment.
In addition, it is used for the detection cartoon of the kit of postoperative patient monitoring in the prior art often by loading pad, pad, tool
It is made up of the carrier film and sample suction pad of detection line and nature controlling line, this kind detection card is adapted only to blood serum sample, and blood plasma and whole blood
Product can not detect, it is necessary to can be detected after centrifugal treating, it is simple and crude in family, home for destitute, condition so to limit kit
Community sanitary etc. occasion application.
The content of the invention
Technical problem to be solved in the utility model is to provide a kind of chemistry of Quantitative detection cardiac troponin
Electrochemiluminescent immunoassay kit.
To solve above technical problem, the utility model adopts the following technical scheme that：
A kind of chemiluminescence immunoassay kit of Quantitative detection cardiac troponin, kit include detection card, hair
Photopolymerization initiation reagent and chemical illumination immunity analysis instrument, detection card include loading pad, chemiluminescence pad, have detection line and matter
Carrier film, sample suction pad, bottom plate and the boosting pad of line are controlled, chemiluminescence pad is included with reference to pad body and coated in pad
Chemical illuminating reagent labelled antibody coating on body, the antibody in chemical illuminating reagent labelled antibody coating is myocardium myo calcium egg
White antibody, detection line are the painting for the pairing antibody or antigen formation that can be combined with the antibody specificity of cardiac troponin
Layer.
According to a preferred aspect of the present utility model, loading pad, chemiluminescence pad, boosting pad, carrier film and suction
Sample pad is interlaced successively to be pasted onto on bottom plate.
According to another preferred aspect of the present utility model, detection card also includes hemofiltration film, and hemofiltration film is covered on loading pad,
Loading pad, chemiluminescence pad, boosting pad, carrier film and sample suction pad is interlaced successively is pasted onto on bottom plate.
Preferably, the material of boosting pad is glass fibre membrane or non-woven fabrics.
Preferably, detection line, nature controlling line are parallel to each other, and detection line is located at the one of the close chemiluminescence pad of carrier film
Side, nature controlling line are located at the side of the close sample suction pad of carrier film.
Preferably, the antibody of cardiac troponin is monoclonal antibody.
Preferably, nature controlling line includes but is not limited to the shapes such as sheep anti-mouse igg, goat-anti chicken IGY or goat anti-rabbit igg by coating
Into.
Preferably, carrier film is nitrocellulose filter and is 5~12um of aperture porous spline structure film.
Preferably, loading pad, the material with reference to pad body are respectively glass fibre membrane or non-woven fabrics, and the material of sample suction pad is
Absorbent filter.
Preferably, chemiluminescence immune assay of the present utility model uses acridinium ester/H2O2System (Acridinium
ester/ H2O2System), chemical illuminating reagent labelled antibody coating is acridinium ester label antibody coating.The luminous reagent that starts corresponds to
For HNO3+ H2O2 And NaOH.
Chemiluminescence immunoassay technology and immuno-chromatographic assay technology are known.Chemistry hair described in the utility model
Light reagent labelled antibody can use method well known in the art to prepare.
Due to the implementation of above technical scheme, the utility model has the following advantages that compared with prior art：This practicality is new
Type uses Quantitative detection cardiac troponin（CTnI）Chemiluminescence immunoassay chromatographic technique, have it is easy to operate, reaction
Quickly, the advantages that sensitivity height, high specificity, for acute myocardial infarction early warning and state of an illness risk judgment.This practicality is new
Type adds boosting pad in structure, can effectively remove ambient interferences, so as to further improve diagnostic sensitivity and accurate
Degree, boosting pad, which additionally aids, in addition accelerates detection sample flow, improves detection efficiency, shortens detection time.
Further, the utility model detection card will can be only applied to the kit of hospital laboratory originally, by technology
Innovation, by increasing hemofiltration film, facilitate it, be simple, being accurately applied to acute myocardial infarction early warning and the state of an illness
Risk judgment, suitable for the simple and crude community sanitary institute of family, home for destitute, condition, its application method is also only by patient or family members
Acupuncture one is bled to realize acute myocardial infarction early warning and state of an illness risk judgment, mitigation goes to hospital to register, is lined up, chemically examining,
The complicated processes such as report are taken, mitigates the pressure of public medical mechanism, is relatively beneficial to the daily treatment of patient.
Brief description of the drawings
Fig. 1 is the structural representation according to detection card of the present utility model；
Wherein：1st, loading pad；2nd, chemiluminescence pad；20th, with reference to pad body；21st, chemical illuminating reagent labelled antibody
Coating；3rd, carrier film；4th, sample suction pad；5th, bottom plate；6th, hemofiltration film；7th, boosting pad；T1, detection line；C, nature controlling line.
Embodiment
As shown in figure 1, a kind of chemiluminescence immunoassay kit of Quantitative detection cardiac troponin, kit include
Detection card, luminous startup reagent and chemical illumination immunity analysis instrument, detection card include loading pad 1, chemiluminescence pad 2, tool
There are carrier film 3, sample suction pad 4, bottom plate 5, hemofiltration film 6 and the boosting pad 7 of detection line and nature controlling line C, hemofiltration film 6 is covered in upper
On sample pad 1, loading pad 1, chemiluminescence pad 2, boosting pad 7, carrier film 3 and sample suction pad 4 is interlaced successively is pasted onto bottom
On plate 5.The material of boosting pad 7 is glass fibre membrane or non-woven fabrics.Carrier film 3 is nitrocellulose filter and is 5~12um of aperture
Porous spline structure film.Loading pad 1, the material with reference to pad body 20 are respectively glass fibre membrane or non-woven fabrics, the material of sample suction pad 4
Matter is absorbent filter.
Further, chemiluminescence pad 2 is included with reference to pad body 20 and coated in the chemistry combined on pad body 20
Luminescence reagent labelled antibody coating 21, the antibody in chemical illuminating reagent labelled antibody coating 21 are the anti-of cardiac troponin
Body, detection line T1 are the coating for the pairing antibody or antigen formation that can be combined with the antibody specificity of cardiac troponin.Inspection
Survey line T1, nature controlling line C are parallel to each other, and detection line T1 is located at the side of the close chemiluminescence pad 2 of carrier film 3, nature controlling line C
Positioned at the side of the close sample suction pad 4 of carrier film 3.The antibody of cardiac troponin is monoclonal antibody.Nature controlling line C passes through coating
The including but not limited to formation such as sheep anti-mouse igg, goat-anti chicken IGY or goat anti-rabbit igg.Chemical illuminating reagent labelled antibody coating is a word used for translation
Pyridine ester labelled antibody coating.The luminous reagent that starts corresponds to HNO3+ H2O2 And NaOH.
Each part of the present utility model is so assemblied together, and loading pad 1, chemistry hair are sequentially bonded on bottom plate 5
Light pad 2, boosting pad 7, carrier film 3 and sample suction pad 4, each several part successively with and be only in contact with adjacent regions and part weight
It is folded, on the loading pad 1 that wherein hemofiltration film 6 is covered in, bar is then cut into, loads and detection card is formed in plastic clip.
The detection method of the utility model when in use is such：
（1）Detection reagent and sample are balanced to room temperature, detection card is taken out, keeps flat；
（2）Serum, blood plasma or whole blood sample are drawn, adds in the centrifuge tube of cleaning, is carried out with Sample dilution (PBS) dilute
Release, fully mix；
（3）Sample after drawing dilution with liquid-transfering gun is added in sample aperture, after 15~20 minutes, sprays luminous startup examination
Agent, by chemical illumination immunity analysis instrument, CTnI concentration is carried out according to the standard curve being set in advance in detector
Result is calculated and be shown.
The utility model Cleaning Principle is as follows：
After sample to be added to the sample well in loading pad 1, the CTnI antigens that contain in sample and acridinium ester label
CTnI antibody bindings, by capillarity, the antibody-antigen immune compound formed is chromatographed to inspection along nitrocellulose filter
Survey line, then the CTnI antibody bindings with being solidificated in detection line.Uncombined immune complex then continues chromatography to nature controlling line
Captured by sheep anti-mouse igg antibody.
Analyzed by the light quantum that chemical illumination immunity analysis instrument collects seizure and detects nature controlling line and detection line, so
Afterwards CTnI concentration is carried out that result is calculated and be shown according to the standard curve being set in advance in detector.
To sum up, the utility model describes Quantitative detection cardiac troponin（CTnI）Chemiluminescence immunoassay chromatography
Technology, have the advantages that easy to operate, rapid reaction, sensitivity be high, high specificity, for acute myocardial infarction early warning and
State of an illness risk judgment.
The utility model detection card will can be only applied to the kit of hospital laboratory originally, by technological innovation, pass through
Increase hemofiltration film, facilitate it, be simple, being accurately applied to acute myocardial infarction early warning and state of an illness risk judgment,
Suitable for the simple and crude community sanitary institute of family, home for destitute, condition, its application method is bled by patient or family members' acupuncture one
To realize acute myocardial infarction early warning and state of an illness risk judgment, mitigation goes to hospital to register, is lined up, chemically examining, taking report etc. numerous
Trivial process, mitigate the pressure of public medical mechanism, be relatively beneficial to the daily treatment of patient.
Further, the utility model adds boosting pad in structure, can effectively remove ambient interferences, so as to enter
One step improves diagnostic sensitivity and the degree of accuracy, and boosting pad additionally aids quickening detection sample flow in addition, improves detection efficiency, contracting
Short detection time.
Further, acridinium ester reagent（Acridinium C2 NSH Ester）For labelled antibody, advantage mainly has：
1. background luminescence is low, signal to noise ratio is high；2. luminescence-producing reaction disturbing factor is few, chemical reaction is simple, it is quick, without catalyst；3. light
The quick concentration of release, luminous efficiency is high, luminous intensity is big；4. it is easy to protein bind and photon yield is not reduced after being coupled；
5. label is stable, the reagent term of validity greatly prolongs.
The utility model is described in detail above, its object is to allow the personage for being familiar with this art can be much of that
Solve content of the present utility model and be carried out, the scope of protection of the utility model can not be limited with this, it is all according to this practicality
The equivalent change or modification that new Spirit Essence is made, it should all cover in the scope of protection of the utility model.
Claims (10)
- A kind of 1. chemiluminescence immunoassay kit of Quantitative detection cardiac troponin, it is characterised in that：The kit Including detection card, luminous startup reagent and chemical illumination immunity analysis instrument, the detection card includes loading pad（1）, chemiluminescence Pad（2）, there is detection line and nature controlling line（C）Carrier film（3）, sample suction pad（4）, bottom plate（5）And boosting pad（7）, institute State chemiluminescence pad（2）Including with reference to pad body（20）With coated in the combination pad body（20）On chemiluminescence examination Agent labelled antibody coating（21）, the chemical illuminating reagent labelled antibody coating（21）In antibody be the cardiac troponin Antibody, the detection line（T1）For the pairing antibody or anti-that can be combined with the antibody specificity of the cardiac troponin Original shape into coating.
- 2. chemiluminescence immunoassay kit according to claim 1, it is characterised in that：The loading pad（1）, chemiluminescence Pad（2）, boosting pad（7）, carrier film（3）And sample suction pad（4）It is interlaced successively to be pasted onto bottom plate（5）On.
- 3. chemiluminescence immunoassay kit according to claim 1, it is characterised in that：The detection card also includes hemofiltration film （6）, the hemofiltration film（6）It is covered in described loading pad（1）On, loading pad（1）, chemiluminescence pad（2）, boosting pad （7）, carrier film（3）And sample suction pad（4）It is interlaced successively to be pasted onto the bottom plate（5）On.
- 4. chemiluminescence immunoassay kit according to claim 1, it is characterised in that：The boosting pad（7）Material be Glass fibre membrane or non-woven fabrics.
- 5. chemiluminescence immunoassay kit according to claim 1, it is characterised in that：The detection line（T1）, nature controlling line （C）It is parallel to each other, the detection line（T1）Positioned at the carrier film（3）The close chemiluminescence pad（2）Side, The nature controlling line（C）Positioned at the carrier film（3）The close sample suction pad（4）Side.
- 6. chemiluminescence immunoassay kit according to claim 1, it is characterised in that：The antibody of the cardiac troponin For monoclonal antibody.
- 7. chemiluminescence immunoassay kit according to claim 1, it is characterised in that：The nature controlling line（C）Pass through coating Sheep anti-mouse igg, goat-anti chicken IGY or goat anti-rabbit igg are formed.
- 8. chemiluminescence immunoassay kit according to claim 1, it is characterised in that：The carrier film（3）It is fine for nitric acid Tie up plain film and for 5~12um of aperture porous spline structure film.
- 9. chemiluminescence immunoassay kit according to claim 1, it is characterised in that：The loading pad（1）, with reference to advance capital for Body（20）Material be respectively glass fibre membrane or non-woven fabrics, the sample suction pad（4）Material be absorbent filter.
- 10. chemiluminescence immunoassay kit according to claim 1, it is characterised in that：The chemical illuminating reagent mark Antibody coating is acridinium ester label antibody coating.
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| CN201721215016.XU CN207123535U (en) | 2017-09-21 | 2017-09-21 | A kind of chemiluminescence immunoassay kit of Quantitative detection cardiac troponin |
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| CN201721215016.XU CN207123535U (en) | 2017-09-21 | 2017-09-21 | A kind of chemiluminescence immunoassay kit of Quantitative detection cardiac troponin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111735960A (en) * | 2019-12-10 | 2020-10-02 | 华南农业大学 | Preparation method of poultry pulmonary hypertension diagnosis kit |
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2017
- 2017-09-21 CN CN201721215016.XU patent/CN207123535U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111735960A (en) * | 2019-12-10 | 2020-10-02 | 华南农业大学 | Preparation method of poultry pulmonary hypertension diagnosis kit |
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