CN207100143U - A kind of adult fish poisonous substance exposure device of small test fish - Google Patents

A kind of adult fish poisonous substance exposure device of small test fish Download PDF

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Publication number
CN207100143U
CN207100143U CN201720292240.2U CN201720292240U CN207100143U CN 207100143 U CN207100143 U CN 207100143U CN 201720292240 U CN201720292240 U CN 201720292240U CN 207100143 U CN207100143 U CN 207100143U
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China
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water
exposure
tank
solution
bath
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CN201720292240.2U
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Chinese (zh)
Inventor
胡晶莹
孙冰
周小羽
王玉柱
田芳
夏敏杰
职瑞娜
李润生
丁训城
李卫华
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Shanghai Institute of biomedical technology
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Shanghai Institute of Planned Parenthood Research
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The utility model provides a kind of adult fish poisonous substance exposure device of small test fish, including open flowing water exposing device, and the open flowing water exposing device includes:Exposure solution supplemental partss, for supplementing exposure solution with tank to exposure;The exposure tank, for accommodating the small test fish and the exposure solution;The exposure tank has delivery port, for discharging the exposure solution.

Description

A kind of adult fish poisonous substance exposure device of small test fish
Technical field
The utility model relates generally to poisonous substance exposure device, more particularly to a kind of adult fish poisonous substance exposure of small test fish is set It is standby.
Background technology
Massive epidemiology is investigated and experimental study confirms, reproductive dysfunction, hormonal dependent organ tumor (such as mammary gland Cancer, uterine cancer, carcinoma of testis, prostate cancer etc.) generation, the generation of deformed child, retarded child all has with environment incretion interferent Close.
The small test such as zebra fish and medaka with fish, is moved as the experiment of Biology of Embryonic Development and science of heredity earliest Thing model, it is widely used in the research of early embryonic development gene expression regulation.From the nineties in last century zebra fish and Medaka is widely used in the acute, chronic of chemicals, Reproductive and developmental toxicity detection, and the bioaccumulation effect of heavy metal Research etc..The research activities that recent domestic carries out environment incretion interferent with small test fish as animal model is fast Speed heating, small test fish have become " bridge " between research environment incretion interferent and human body relevant disease. In the whole life cycle of small test fish, the biological effect of environmental poisonous substance can be by the death rate, growth inhibition shape The detection of condition, development toxicity, neurotoxicity and genotoxicity etc. and embody.
At present, environment incretion interferent is to the exposure experimental provision of small test fish adult fish, is broadly divided into flowing water, half quiet Three kinds of water, hydrostatic exposed modes.Continuous-flow type exposure is more advanced exposed mode at present, in the control of stability of concentration with And reduce human input etc. and be better than half hydrostatic and hydrostatic pattern.However, existing continuous-flow type exposing device is using process In, however it remains the unstable situation of exposure solution concentration.
Utility model content
Inventors herein have recognized that existing continuous-flow type exposing device is circulating, even if the exposure after using is molten Liquid reuses after filtering, cause continuous-flow type exposing device exist the main reason for concentration unstable situation be filter simultaneously Can not complete impurity screening, and the concentration of exposure solution after filtering can also produce change, by the exposure solution after filtering again Secondary use eventually introduces impurity, and causes concentration unstable.Based on this discovery, the utility model provides a kind of open stream Water exposing device, it has the characteristics of making the concentration of exposure solution more stable, accurate.
The utility model provides a kind of adult fish poisonous substance exposure device of small test fish, including open flowing water exposure dress Put, the open flowing water exposing device includes:Exposure solution supplemental partss, for supplementing exposure solution with tank to exposure;Should Exposure tank, for accommodating the small test fish and the exposure solution;The exposure tank has delivery port, for discharging this Exposure solution.
In an embodiment of the present utility model, the exposure solution supplemental partss include:Exposure solution accumulator tank, for storing up Deposit the exposure solution;Exposure solution water inlet pipe, it is connected with the exposure solution accumulator tank, for by the exposure solution accumulator tank The exposure solution is guided into the exposure tank.
In an embodiment of the present utility model, syringe pump is provided with the exposure solution water inlet pipe, it is sudden and violent for adjusting this Reveal the flow of the exposure solution in solution water inlet pipe.
In an embodiment of the present utility model, the open flowing water exposing device also includes:Water purification supplemental partss, are used for Coordinate with the exposure solution supplemental partss, adjust the concentration of the exposure solution, to supplement exposure solution with tank to the exposure.
In an embodiment of the present utility model, the water purification supplemental partss include:Water purifier, it is net for being carried out to water inlet Change;Water storage tank, for store it is purified after water purification;Water purification water inlet pipe, connect respectively with the water storage tank, the exposure solution water inlet pipe Connect, for water purification to be injected into the exposure solution water inlet pipe, to adjust the concentration of the exposure solution.
In an embodiment of the present utility model, water pump is provided with the water purification water inlet pipe, it is sudden and violent to be injected into this for adjustment Reveal the flow of the water purification in solution water inlet pipe.
In an embodiment of the present utility model, the open flowing water exposing device also includes exposure solution recovery part, For collecting, filtering from the exposure with the exposure solution discharged in tank.
In an embodiment of the present utility model, the exposure solution recovery part includes:Liquid case is reclaimed, for collecting from this Expose with the exposure solution discharged in tank;Case is attenuated, is connected with the recovery liquid case, for filtering being somebody's turn to do in the recovery liquid case Exposure solution, to discharge after being attenuated.
In an embodiment of the present utility model, in addition to water proof temperature control device, for making the exposure tank be in perseverance In tepidarium media environment.
In an embodiment of the present utility model, the water proof temperature control device includes:Bath water groove, there is an opening and a water outlet Mouthful, for accommodating the exposure tank and water-bath medium;Water-bath water tank, for accommodating the water-bath medium, and this is heated or cooled Water-bath medium;Water-bath pump, it is connected with water-bath water inlet pipe, for the water-bath medium in the water-bath water tank to be intake by the water-bath Pipe is injected into the bath water groove through the opening;Water-bath outlet pipe, it is connected with the delivery port of the bath water groove, for will be through the delivery port The water-bath medium of outflow is guided to the water-bath water tank;And temperature control unit, for detecting the water-bath in the bath water groove The temperature of medium, and control the water-bath water tank is heated or cooled behavior, to adjust the temperature of the water-bath medium.
Compared with prior art, the utility model has advantages below:Open flowing water exposing device of the present utility model, The exposure solution for being exposed through being discharged with the delivery port of tank directly or after processing is discharged, and is not re-introduced into weight in exposure tank It is multiple to use, in this way, the adult fish poisonous substance exposure device of small test fish of the present utility model is compared to existing circulating continuous-flow type Exposing device has the characteristics of concentration of exposure solution is more stable, accurate.Exposure solution accumulator tank of the present utility model, exposure The container made of tank is glass material, exposure solution water inlet pipe are stainless steel tube, compared to it is existing by polyvinyl chloride, it is poly- Container, tubing made of the plastic materials such as ethene, polystyrene, in the absence of due to that itself may contain on a small quantity in plastic products Environment incretion interferent, the situation that can be impacted to the experimental result of environment incretion interferent, further ensures reality Test the accuracy of result.The utility model is integrated water proof temperature control device and open flowing water exposing device, makes small-sized reality Test that the temperature of the exposed environments of the adult fish of fish is more constant, reduce environment temperature to the influence caused by experimental result.
Brief description of the drawings
Fig. 1 is the structural representation of the adult fish poisonous substance exposure device of the small test fish of the utility model first embodiment.
Fig. 2 is the structural representation of the adult fish poisonous substance exposure device of the small test fish of the utility model second embodiment.
Fig. 3 is the structural representation of the adult fish poisonous substance exposure device of the small test fish of the utility model 3rd embodiment.
Fig. 4 is the structural representation of the adult fish poisonous substance exposure device of the small test fish of the utility model fourth embodiment.
Fig. 5 is the structural representation of the adult fish poisonous substance exposure device of the small test fish of the embodiment of the utility model the 5th.
Embodiment
For above-mentioned purpose of the present utility model, feature and advantage can be become apparent, below in conjunction with accompanying drawing to this practicality New embodiment elaborates.
Many details are elaborated in the following description in order to fully understand the utility model, but this practicality is new Type can also be different from other manner described here to implement using other, therefore the utility model is not by following public tool The limitation of body embodiment.
As shown in the application and claims, unless context clearly prompts exceptional situation, " one ", "one", " one The word such as kind " and/or "the" not refers in particular to odd number, may also comprise plural number.It is, in general, that term " comprising " is only prompted to wrap with "comprising" Include clearly identify the step of and element, and these steps and element do not form one it is exclusive enumerate, method or equipment The step of may also including other or element.
Fig. 1 is the structural representation of the adult fish poisonous substance exposure device of the small test fish of the utility model first embodiment. With reference to figure 1, the adult fish poisonous substance exposure device 100 of small test fish includes open flowing water exposing device 110.Open flowing water is sudden and violent Dew device 110 includes exposure solution supplemental partss 111 and tank 112 is used in exposure.
Exposure solution supplemental partss 111 are used to supplement exposure solution with tank 112 to exposure.Exposure solution supplemental partss 111 can include exposure solution accumulator tank 111a and exposure solution water inlet pipe 111b.
Exposure solution accumulator tank 111a is used to store the exposure solution.In the embodiment shown in fig. 1, exposure solution is supplemented Part 111 has six exposure solution accumulator tank 111a, it is possible to understand that, the utility model is to exposure solution accumulator tank 111a's Number does not limit, can be set with experimental demand, such as with one, two or ten etc..It can equally manage Solution, multiple exposure solution accumulator tank 111a can store different exposure solutions respectively, it is possibility to have section store is phase Same exposure solution, can also be all storages identical exposure solutions.Wherein, it is described in the utility model " different Exposure solution " can be the solution of different solubility and/or different poisonous substances.Preferably, exposure solution accumulator tank 111a is glass material The accumulator tank of matter.
Exposure solution water inlet pipe 111b is connected with exposure solution accumulator tank 111a, for by exposure solution accumulator tank 111a Exposure solution guide into exposure tank 112.In the embodiment shown in fig. 1, in each exposure solution water inlet pipe 111b Exposure solution four exposure tanks 112 are separately directed to after shunting, it is possible to understand that, the utility model is to the shunting Number does not limit, can be set with experimental demand, such as with one, two or ten etc..In addition, exposure is molten Liquid water inlet pipe 111b can be provided with syringe pump 111c, for adjusting the stream of the exposure solution in exposure solution water inlet pipe 111b Amount.Preferably, exposure solution water inlet pipe 111b is stainless steel tube.
Exposure tank 112 is used to accommodate small test fish and exposure solution.Exposure has delivery port with tank 112 112a, for discharging exposure solution.Preferably, exposure tank 112 is the tank of glass material.
In the embodiment shown in fig. 1, there are open flowing water exposing device 110 three rows to expose with tank 112 (wherein, Second and third row is not drawn into exposure tank 112), often row is respectively arranged two groups of exposure tanks 112, shares six groups of exposure water Groove 112.It should be understood that the row and group number of open flowing water exposing device 110 can be adjusted with experimental demand Whole, the utility model is not limited thereto.
Open flowing water exposing device 110 of the present utility model, it is exposed through being discharged with the delivery port 112a of tank 112 sudden and violent Reveal solution, discharge directly or after processing, be not re-introduced into exposure and reused with tank 112, in this way, of the present utility model The adult fish poisonous substance exposure device 100 of small test fish has exposure solution compared to existing circulating continuous-flow type exposing device The characteristics of concentration is more stable, accurate.In addition, exposure solution accumulator tank 111a of the present utility model, exposure tank 112 are glass Container made of glass material, exposure solution water inlet pipe 111b are stainless steel tube, compared to it is existing by polyvinyl chloride, polyethylene, Container, tubing made of the plastic materials such as polystyrene, in the absence of due in plastic products itself a small amount of environment may be contained Incretion interferent, the situation that can be impacted to the experimental result of environment incretion interferent, further ensure experiment knot The accuracy of fruit.
Fig. 2 is the structural representation of the adult fish poisonous substance exposure device of the small test fish of the utility model second embodiment. The adult fish poisonous substance exposure device 200 of small test fish illustrated in fig. 2 is sudden and violent with the adult fish poisonous substance of small test fish illustrated in fig. 1 The difference of dew equipment 100 is that its open flowing water exposing device 110 adds water purification supplemental partss 113.Therefore, herein not Exposure solution supplemental partss 111 and exposure tank 112 are described in detail again, and water purification supplemental partss are only described in detail 113。
Water purification supplemental partss 113 are used to coordinate with exposure solution supplemental partss 111, the concentration of exposure solution are adjusted, with right Exposure supplements exposure solution with tank 112.Water purification supplemental partss 113 can include water purifier 113a, water storage tank 113b and water purification Water inlet pipe 113c.Water purifier 113a is used to purify water inlet.Water storage tank 113b be used for store it is purified after water purification.Water purification Water inlet pipe 113c is connected respectively with water storage tank 113b, exposure solution water inlet pipe 111b, is entered for water purification to be injected into exposure solution In water pipe 111b, to adjust the concentration of the exposure solution.Water pump 113d can be provided with water purification water inlet pipe 113c, for adjusting The flow for the water purification being injected into exposure solution water inlet pipe 111b.
, can be with although in the embodiment shown in Figure 2, water pump 113d is provided at water purification water inlet pipe 113c house steward Understand, equally can water purification water inlet pipe 113c it is each be in charge of set water pump 113d, with individually to it is each be in charge of it is net Water-carrying capacity is controlled.
Fig. 3 is the structural representation of the adult fish poisonous substance exposure device of the small test fish of the utility model 3rd embodiment. The adult fish poisonous substance exposure device 300 of small test fish illustrated in fig. 3 is sudden and violent with the adult fish poisonous substance of small test fish illustrated in fig. 1 The difference of dew equipment 100 is that its open flowing water exposing device 110 adds exposure solution recovery part 114.Therefore, exist No longer exposure solution supplemental partss 111 and exposure tank 112 are described in detail for this, and exposure solution is only described in detail and returns Receive part 114.
Exposure solution recovery part 114 is used to collect, filtered from exposed with the exposure solution discharged in tank 112.Exposure Solution recovery part 114 can include recovery liquid case 114a and attenuation case 114b.Liquid case 114a is reclaimed to be used to collect from exposure use The exposure solution discharged in tank 112.Attenuation case 114b is connected with recovery liquid case 114a, for being recovered by filtration in liquid case 114a Exposure solution, to discharge after being attenuated.
Fig. 4 is the structural representation of the adult fish poisonous substance exposure device of the small test fish of the utility model fourth embodiment. The adult fish poisonous substance exposure device 400 of small test fish illustrated in fig. 4 is sudden and violent with the adult fish poisonous substance of small test fish illustrated in fig. 1 The difference of dew equipment 100 is that it also has water proof temperature control device 120.Therefore, herein no longer to open flowing water exposing device 110 are described in detail, and water proof temperature control device 120 is only described in detail.
Water proof temperature control device 120 is used to make exposure tank 112 be in water bath with thermostatic control media environment.Water proof temperature control device 120 can include bath water groove 121, water-bath water tank 122, water-bath pump 123, water-bath water inlet pipe 124, water-bath outlet pipe 125 and temperature Spend control unit 126.Bath water groove 121 has an an opening 121a and delivery port 121b, for accommodating exposure tank 112 and water Bathe medium.Water-bath water tank 122 is used to accommodate water-bath medium, and water-bath medium is heated or cooled.Water-bath pump 123 and water-bath water inlet pipe 124 connections, for the water-bath medium in water-bath water tank 122 to be injected into bath water groove through opening 121a by water-bath water inlet pipe 124 In 121.Water-bath outlet pipe 125 is connected with the delivery port 121b of bath water groove 121, for the water-bath matchmaker that will be flowed out through delivery port 121b It is situated between and guides to water-bath water tank 122.Temperature control unit 126 is used for the temperature for detecting the water-bath medium in bath water groove 121, and controls Behavior is heated or cooled in water-bath water tank 122, to adjust the temperature of water-bath medium.
The present embodiment is integrated water proof temperature control device 120 and open flowing water exposing device 110, makes small test fish Adult fish exposed environments temperature it is more constant, to reduce environment temperature to the influence caused by experimental result.
Fig. 5 is the structural representation of the adult fish poisonous substance exposure device of the small test fish of the embodiment of the utility model the 5th. The adult fish poisonous substance exposure device 500 of small test fish illustrated in fig. 5 has open flowing water exposing device 110 and water proof temperature control Device 120, wherein there is open flowing water exposing device 110 exposure solution supplemental partss 111, exposure tank 112, water purification to mend Part 113 and exposure solution recovery part 114 are filled, these parts are identical with foregoing embodiment, water proof temperature control device 120 It is identical with embodiment illustrated in fig. 4, no longer these parts are described in detail herein.
Although the utility model describes with reference to current specific embodiment, ordinary skill in the art Personnel are it should be appreciated that the embodiment of the above is intended merely to illustrate the utility model, without departing from the utility model spirit In the case of can also make various equivalent change or replacement, therefore, as long as to upper in spirit of the present utility model State the change of embodiment, modification will all fall in the range of following claims.

Claims (10)

1. the adult fish poisonous substance exposure device of a kind of small test fish, it is characterised in that including open flowing water exposing device, this is opened Putting formula flowing water exposing device includes:
Exposure solution supplemental partss, for supplementing exposure solution with tank to exposure;
The exposure tank, for accommodating the small test fish and the exposure solution;The exposure tank has delivery port, is used for Discharge the exposure solution.
2. equipment according to claim 1, it is characterised in that the exposure solution supplemental partss include:
Exposure solution accumulator tank, for storing the exposure solution;
Exposure solution water inlet pipe, it is connected with the exposure solution accumulator tank, for the exposure in the exposure solution accumulator tank is molten Liquid is guided into the exposure tank.
3. equipment according to claim 2, it is characterised in that be provided with syringe pump on the exposure solution water inlet pipe, be used for Adjust the flow of the exposure solution in the exposure solution water inlet pipe.
4. equipment according to claim 2, it is characterised in that the open flowing water exposing device also includes:Water purification supplements Part, for coordinating with the exposure solution supplemental partss, the concentration of the exposure solution is adjusted, it is sudden and violent to be supplemented with tank the exposure Reveal solution.
5. equipment according to claim 4, it is characterised in that the water purification supplemental partss include:
Water purifier, for being purified to water inlet;
Water storage tank, for store it is purified after water purification;
Water purification water inlet pipe, it is connected respectively with the water storage tank, the exposure solution water inlet pipe, for water purification to be injected into the exposure solution In water inlet pipe, to adjust the concentration of the exposure solution.
6. equipment according to claim 5, it is characterised in that be provided with water pump on the water purification water inlet pipe, noted for adjusting Enter the flow of the water purification into the exposure solution water inlet pipe.
7. equipment according to claim 1, it is characterised in that the open flowing water exposing device also returns including exposure solution Part is received, for collecting, filtering from the exposure with the exposure solution discharged in tank.
8. equipment according to claim 7, it is characterised in that the exposure solution recovery part includes:
Liquid case is reclaimed, for collecting from the exposure with the exposure solution discharged in tank;
Case is attenuated, is connected with the recovery liquid case, for filtering the exposure solution in the recovery liquid case, to carry out attenuation heel row Put.
9. equipment according to claim 1, it is characterised in that also including water proof temperature control device, for making the exposure water Groove is in water bath with thermostatic control media environment.
10. equipment according to claim 9, it is characterised in that the water proof temperature control device includes:
Bath water groove, there is an opening and a delivery port, for accommodating the exposure tank and water-bath medium;
Water-bath water tank, for accommodating the water-bath medium, and the water-bath medium is heated or cooled;
Water-bath pump, it is connected with water-bath water inlet pipe, for the water-bath medium in the water-bath water tank to be passed through by the water-bath water inlet pipe The opening is injected into the bath water groove;
Water-bath outlet pipe, be connected with the delivery port of the bath water groove, for by the water-bath medium flowed out through the delivery port guide to The water-bath water tank;And
Temperature control unit, for detecting the temperature of the water-bath medium in the bath water groove, and control the heating of the water-bath water tank Or cooling behavior, to adjust the temperature of the water-bath medium.
CN201720292240.2U 2017-03-23 2017-03-23 A kind of adult fish poisonous substance exposure device of small test fish Active CN207100143U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720292240.2U CN207100143U (en) 2017-03-23 2017-03-23 A kind of adult fish poisonous substance exposure device of small test fish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720292240.2U CN207100143U (en) 2017-03-23 2017-03-23 A kind of adult fish poisonous substance exposure device of small test fish

Publications (1)

Publication Number Publication Date
CN207100143U true CN207100143U (en) 2018-03-16

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201720292240.2U Active CN207100143U (en) 2017-03-23 2017-03-23 A kind of adult fish poisonous substance exposure device of small test fish

Country Status (1)

Country Link
CN (1) CN207100143U (en)

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Address after: 200032 Shanghai Xuhui District Xietu Road No. 2140

Patentee after: Shanghai Institute of biomedical technology

Address before: 200032 No. 2140 Xietu Road, Xuhui District, Shanghai

Patentee before: SHANGHAI INSTITUTE OF PLANNED PARENTHOOD RESEARCH