CN207042924U - A kind of immunoblotting Membrane cleaning device - Google Patents
A kind of immunoblotting Membrane cleaning device Download PDFInfo
- Publication number
- CN207042924U CN207042924U CN201720764176.3U CN201720764176U CN207042924U CN 207042924 U CN207042924 U CN 207042924U CN 201720764176 U CN201720764176 U CN 201720764176U CN 207042924 U CN207042924 U CN 207042924U
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- China
- Prior art keywords
- liquid
- delivery port
- liquid bath
- outlet
- cleaning device
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Abstract
A kind of immunoblotting Membrane cleaning device is the utility model is related to, device for storing liquid is connected to washing trough by conduit, and concatenation can adjust water pump on conduit；The liquid outlet of drain pipe connection device for storing liquid and the entrance of adjustable water pump of conduit, the outlet connection liquid-in pipe of adjustable water pump, liquid-in pipe separates two-way, the first delivery port and the second delivery port being connected respectively on the inside of the gear liquid bath of washing trough, the water outlet direction of first delivery port and the second delivery port is not on the contrary, and conllinear；The bottom plate and gear liquid bath of washing trough form concave shaped, and the inner side for keeping off liquid bath is provided with gap, gap and the conducting of groove out outlet.The utility model regulating flow quantity, fully effectively wash, be easy to operate.
Description
Technical field
Membrane cleaning engineering device technique field is the utility model is related to, particularly a kind of regulating flow quantity, is fully effectively washed, behaviour
Make easy immunoblotting Membrane cleaning device.
Background technology
In life science, Western Blot are the most commonly used for detecting certain albumen in cell or tissue
One of method.Protein delivery after typically cell or tissue is cracked is total to on PVDF or NC films with specific antibody with film
It is incubated together, then the expression of corresponding albumen is shown by special coloration method.Flow is：The preparation of protein sample,
SDS-PAGE electrophoresis, transferring film, closing, primary antibody are incubated, and wash film, and secondary antibody is incubated, and wash film, substrate colour developing, analysis.
In operation, have and wash membrane process twice, it is therefore an objective to remove the Excess antibody for being adhered to film surface.It is common
It is that film is placed in container to wash film method, adds buffer solution, is rocked on shaking table.Each film link is washed at least to wash three times, every time
No less than 10 minutes.Following defect be present in this method：Complex operation, by film transposition in container, washing one secondary will wash
Liquid is gone, and changes fresh buffer again, and these operations are intended to manually carry out.It is time-consuming, each wash film link at least 30 minutes.
The experiment rush hour, the film time was washed in extension often because forgetting to change liquid.Influence operating table surface cleaning：Container for washing film is more
It is open, process of rocking often has liquid spilling, influences table top cleaning.
Need a kind of regulating flow quantity, fully effectively wash, immunoblotting Membrane cleaning device easy to operate.
Utility model content
The purpose of this utility model is to provide a kind of regulating flow quantity, fully effectively washing, protein easy to operate print
Mark method Membrane cleaning device.
A kind of immunoblotting Membrane cleaning device, including：
Device for storing liquid, the device for storing liquid are connected to washing trough by conduit, and concatenation can adjust water pump on the conduit；
The liquid outlet of drain pipe connection device for storing liquid and the entrance of adjustable water pump of the conduit, the adjustable water pump
Outlet connection liquid-in pipe, the liquid-in pipe separates two-way, the first delivery port for being connected respectively on the inside of the gear liquid bath of washing trough and
Second delivery port, the water outlet direction of first delivery port and the second delivery port is not on the contrary, and conllinear；
The bottom plate and gear liquid bath of the washing trough form concave shaped, and the inner side of the gear liquid bath is provided with gap, the spilling water
Mouth and the conducting of groove out outlet.
The top of the gear liquid bath is provided with cover plate, and the cover plate passes through sealing ring fixing seal.
The adjustable water pump is provided with the knob of regulation flow.
It is two or more that the bottom plate and gear liquid bath, which form concave shaped quantity, and each concave shaped is provided with one group of first water outlet
Mouth, the second delivery port and gap.
The gear liquid bath is the cabling channel of inner hollow, and the liquid-in pipe is entered by keeping off the through hole on the outside of liquid bath, enters liquid
Pipe is placed in the inside of gear liquid bath, and outlet is respectively aligned to the first delivery port and the second delivery port.
The concave shaped that the bottom plate and gear liquid bath are formed is rectangle.
The utility model device for storing liquid is connected to washing trough by conduit, and concatenation can adjust water pump on conduit；Conduit goes out liquid
The liquid outlet of pipe connection device for storing liquid and the entrance of adjustable water pump, it can adjust the outlet connection liquid-in pipe of water pump, liquid-in pipe point
Go out two-way, the first delivery port and the second delivery port being connected respectively on the inside of the gear liquid bath of washing trough, the first delivery port and second go out
The water outlet direction at the mouth of a river is not on the contrary, and conllinear；The bottom plate and gear liquid bath of washing trough form concave shaped, and the inner side for keeping off liquid bath is provided with spilling water
Mouthful, gap and the conducting of groove out outlet.Have in washing trough frame conduit can the water filling into groove, the particular design of delivery port can be with
Ensure to form vortex in groove, be advantageous to the flushing to film, delivery port enters water using inclined-plane in groove, can ensure shape in groove during injection
Into unimodal current, realize and blotting membrane is fully washed.Adjustable water pump can control single groove or double flute water filling, and water flow velocity
Degree.The utility model regulating flow quantity, fully effectively wash, be easy to operate.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model；
Fig. 2 is the structural representation of the utility model washing trough；
Fig. 3 is the stereogram of the utility model cover plate；
Fig. 4 is front view of the present utility model；
In figure：1st, device for storing liquid, 2, conduit, 3, adjustable water pump, 4, washing trough, 5, cover plate, 6, sealing ring, 21, drain pipe,
22nd, liquid-in pipe, the 23, first delivery port, the 24, second delivery port, 41, bottom plate, 42, gear liquid bath, 43, gap, 44, the outer water outlet of groove
Mouthful.
Embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is described further.
Embodiment 1：
A kind of immunoblotting Membrane cleaning device, including：Device for storing liquid 1, device for storing liquid 1 are connected to by conduit 2 and washed
Groove 4, the adjustable water pump 3 of concatenation on conduit 2；The drain pipe 21 of conduit 2 connects the liquid outlet and adjustable water pump 3 of device for storing liquid 1
Entrance, can adjust the outlet connection liquid-in pipe 22 of water pump 3, liquid-in pipe 22 separates two-way, be connected respectively to the gear liquid bath of washing trough 4
The first delivery port 23 and the second delivery port 24 of 42 inner sides, the water outlet direction of the first delivery port 23 and the second delivery port 24 on the contrary,
It is and not conllinear；The bottom plate 41 and gear liquid bath 42 of washing trough 4 form concave shaped, and the inner side of gear liquid bath 42 is provided with gap 43, gap
43 and groove out outlet 44 turn on.
The top of gear liquid bath 42 is provided with cover plate 5, and cover plate 5 passes through the fixing seal of sealing ring 6.Adjustable water pump 3, which is provided with, to be adjusted
The knob of amount of restriction.It is two or more that bottom plate 41 and gear liquid bath 42, which form concave shaped quantity, and each concave shaped is provided with one group first
Delivery port 23, the second delivery port 24 and gap 43.The cabling channel that liquid bath 42 is inner hollow is kept off, liquid-in pipe 22 is by keeping off liquid bath
The through hole in 42 outsides enters, and liquid-in pipe 22 is placed in the inside of gear liquid bath 42, and outlet is respectively aligned to the first delivery port 23 and second and gone out
The mouth of a river 24.The concave shaped that bottom plate 41 and gear liquid bath 42 are formed is rectangle.
Have in the frame of washing trough 4 conduit 2 can the water filling into groove, the particular design of delivery port can ensure to form whirlpool in groove
Stream, is advantageous to the flushing to film.The cover plate 5 of Simple transparent can situation of the film in washing trough directly perceived.Adjustable water pump 3 can be controlled
Make single groove or double flute water filling, and water velocity.
The advantages of the utility model：
It can ensure to form unimodal current in groove 1. delivery port enters water using inclined-plane in groove, during injection, realize to blotting membrane
Fully washing.
2. washed using single groove or double flute as needed simultaneously, the use of buffer solution is saved.
3. water flow controller can adjust water velocity, washing intensity is increased or decreased.
4. it is easy to operate, save time and manpower.
General principle, principal character and advantage of the present utility model has been shown and described above.The technical staff of the industry
It should be appreciated that the utility model is not restricted to the described embodiments, the simply explanation described in above-described embodiment and specification is originally
The principle of utility model, on the premise of the spirit and scope of the utility model is not departed from the utility model also have various change and
Improve, these changes and improvements are both fallen within claimed the scope of the utility model.The utility model requires protection scope by
Appended claims and its equivalent define.
Claims (6)
- A kind of 1. immunoblotting Membrane cleaning device, it is characterised in that including：Device for storing liquid (1), the device for storing liquid (1) are connected to washing trough (4) by conduit (2), concatenated on the conduit (2) adjustable Water saving pump (3)；The liquid outlet of drain pipe (21) connection device for storing liquid (1) and the entrance of adjustable water pump (3) of the conduit (2), it is described The outlet connection liquid-in pipe (22) of adjustable water pump (3), the liquid-in pipe (22) separate two-way, are connected respectively to washing trough (4) The first delivery port (23) and the second delivery port (24) kept off on the inside of liquid bath (42), first delivery port (23) and the second delivery port (24) water outlet direction is not on the contrary, and conllinear；The bottom plate (41) and gear liquid bath (42) of the washing trough (4) form concave shaped, and the inner side of the gear liquid bath (42) is provided with spilling water Mouth (43), the gap (43) and groove out outlet (44) conducting.
- A kind of 2. immunoblotting Membrane cleaning device according to claim 1, it is characterised in that the gear liquid bath (42) Top be provided with cover plate (5), the cover plate (5) passes through sealing ring (6) fixing seal.
- A kind of 3. immunoblotting Membrane cleaning device according to claim 1, it is characterised in that the adjustable water pump (3) it is provided with the knob of regulation flow.
- A kind of 4. immunoblotting Membrane cleaning device according to claim 1, it is characterised in that the bottom plate (41) and It is two or more to keep off liquid bath (42) and form concave shaped quantity, and each concave shaped is provided with one group of first delivery port (23), the second water outlet Mouth (24) and gap (43).
- A kind of 5. immunoblotting Membrane cleaning device according to claim 1, it is characterised in that the gear liquid bath (42) For the cabling channel of inner hollow, the liquid-in pipe (22) is entered by keeping off the through hole on the outside of liquid bath (42), and liquid-in pipe (22) is placed in The inside of liquid bath (42) is kept off, outlet is respectively aligned to the first delivery port (23) and the second delivery port (24).
- A kind of 6. immunoblotting Membrane cleaning device according to claim 1, it is characterised in that the bottom plate (41) and It is rectangle to keep off the concave shaped that liquid bath (42) is formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720764176.3U CN207042924U (en) | 2017-06-28 | 2017-06-28 | A kind of immunoblotting Membrane cleaning device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720764176.3U CN207042924U (en) | 2017-06-28 | 2017-06-28 | A kind of immunoblotting Membrane cleaning device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207042924U true CN207042924U (en) | 2018-02-27 |
Family
ID=61493292
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720764176.3U Expired - Fee Related CN207042924U (en) | 2017-06-28 | 2017-06-28 | A kind of immunoblotting Membrane cleaning device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207042924U (en) |
-
2017
- 2017-06-28 CN CN201720764176.3U patent/CN207042924U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180227 Termination date: 20200628 |