CN207031420U - Cell pretreatment device - Google Patents
Cell pretreatment device Download PDFInfo
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- CN207031420U CN207031420U CN201720939674.7U CN201720939674U CN207031420U CN 207031420 U CN207031420 U CN 207031420U CN 201720939674 U CN201720939674 U CN 201720939674U CN 207031420 U CN207031420 U CN 207031420U
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Abstract
The utility model discloses a kind of cell pretreatment device, and it includes casing, cell sample processing pond, micro-filtration pond, UF membrane pond, waste liquid collection box, flexible pipe and small molecule collection vessel.Top plate is embedded to be cased with electromagnet one and upper electromagnet two；Cell sample processing pond bottom is connected by spring one with bottom plate, and bottom plate is embedded to be cased with lower electromagnet one and lower electromagnet two；Micro-filtration bottom of pond portion is connected by spring two with bottom plate, and filtering cylinder is inserted with UF membrane pond, and the bottom for filtering cylinder is provided with seperation film；Pump and magnetic valve are provided with every flexible pipe.On the one hand the utility model adapts to gravity environment and microgravity environment, on the other hand, sample treatment film, microfiltration membranes and seperation film and flexible pipe can be not easily blocked, lysis efficiency is high, and cleavage rate is high；And macromolecular and separation of small molecuies efficiency high.
Description
Technical field
Biological plant manufacturing engineering field is the utility model is related to, particularly a kind of cell pretreatment device.
Background technology
In current bioscience research field, utilization space microgravity environment carries out cell culture, biological sample separation
The research of purifying, the analysis detection of various biological samples etc., not only with great scientific research value, and with extensive
Commercial prospect.
Under space environment, to cell processing procedure used by centrifugation, extraction, precipitation etc. operation by microgravity shadow
Ring and can not be normally carried out.Therefore need to design new mode to process the biological samples such as cell, with separation and Extraction purpose
Product.Due to the harsh conditions under space microgravity environment, cell pretreatment and separation of biopolymer process are very difficult,
A kind of device pre-processed under microgravity environment to cell is also lacked in the prior art.
Beijing Institute of Technology is in the Chinese utility model patent Shen of the Application No. 201410844513.0 of application in 2014
Please, entitled " a kind of cell pretreatment apparatus and method " that its utility model is created, it includes：Pending cell sample storage
Area, buffer solution memory block one, lysate memory block, liquid delivery member one, liquid delivery member two, liquid delivery member three,
Cell sample processing pond, micro-filtration pond, liquid delivery member four, UF membrane pond, small molecule collecting region, buffer solution memory block two, liquid
Body transfer unit five, macromolecular collecting region.Pending cell sample memory block, buffer solution memory block one, lysate memory partitioning
The entrance of cell sample processing pond is not connected by liquid delivery member one, liquid delivery member two, liquid delivery member three；
The entrance in the outlet connection micro-filtration pond of cell sample processing pond；The outlet in micro-filtration pond connects UF membrane by liquid delivery member four
The entrance in pond；The outlet in the UF membrane pond is also connected with the entrance of small molecule collecting region.Buffer solution memory block two is defeated by liquid
The outlet of the junctional membrane separate tank of part five is sent, the entrance in the UF membrane pond is also connected with the entrance of macromolecular collecting region.
Above-mentioned patent application, which is throughout managed the promotion in device using liquid delivery member and switched, carries out cell sample
The pretreatments such as cleaning, cracking and material separation, the device can carry out the pretreatment of cell sample on ground and microgravity environment
Process, solve difficulty of the cell sample under space environment, realize the automation pretreatment of cell sample, to a certain extent
The cell lysis efficiency and the macromolecular rate of recovery improved.However it remains following deficiency：
During 1. cell sample processing pond is cracked, make on sample treatment film treat cell lysis sample with
Lysate to shake back and forth between cell sample processing pond, liquid delivery member three and lysate memory block together, about
20-40 minutes.This setup, there is following deficiency：Cell lysis is treated, is often shaken once, part is just had and waits to crack
Cell is adsorbed on sample treatment film and transfer unit three, on the one hand, the time of contact for treating cell lysis and lysate can be reduced,
Make the reduction of cell cleavage rate.On the other hand, adsorb and treat cell lysis on sample treatment film and transfer unit three, prolong with the time
It is long, the blocking of sample treatment film and the pipeline of transfer unit three can be caused, further reduce lysis efficiency and cleavage rate.
2. macromolecular is trapped in seperation film by UF membrane pond when carrying out macromolecular and separation of small molecuies, then to cutting
The macromolecular stayed in seperation film is collected recovery, on the one hand complicated, on the other hand, big point be trapped in seperation film
Son easily blocks seperation film, so that the separation of macromolecular and small molecule is not complete enough, separating effect reduces.
Utility model content
The technical problems to be solved in the utility model is to be directed to above-mentioned the deficiencies in the prior art, and provides a kind of cell and locate in advance
Device is managed, on the one hand the cell pretreatment device adapts to gravity environment and microgravity environment, on the other hand, can cause at sample
Reason film, microfiltration membranes and seperation film and flexible pipe are not easily blocked, and lysis efficiency is high, and cleavage rate is high；And macromolecular and separation of small molecuies
Efficiency high.
In order to solve the above technical problems, the technical solution adopted in the utility model is：
A kind of cell pretreatment device, including casing, cell sample processing pond, micro-filtration pond, UF membrane pond, waste liquid recovery
Case, flexible pipe and small molecule collection vessel；Wherein, cell sample processing pond, micro-filtration pond, UF membrane pond, waste liquid collection box and small
Molecular collection container is arranged in casing.
Casing includes top plate, bottom plate and side plate, wherein, top plate is connected by side plate with bottom plate.
Cell sample storage tank to be handled, buffer solution are set gradually on the top plate directly over cell sample processing pond
Storage tank and lysate storage tank.
Top plate directly over cell sample processing pond, which embeds, is cased with upper electromagnet one.
Cell sample entrance, buffer inlet and lysate entrance are disposed with the top of cell sample processing pond.
The outlet of pending cell sample storage tank enters with cell sample entrance, the outlet of buffer solution storage tank and buffer solution
Connected between mouth and the outlet of lysate storage tank and lysate entrance by flexible pipe.
Cell sample processing pond bottom is connected by spring one with bottom plate, the bottom immediately below cell sample processing pond
Plate is embedded to be cased with lower electromagnet one.
Sample treatment film is provided with cell sample processing pond, the cell sample processing pond below sample treatment film
Waste liquid outlet and lysate outlet are respectively arranged with pool wall；Wherein, waste liquid outlet passes through flexible pipe and waste liquid collection box
It is connected.
Micro-filtration bottom of pond portion is connected by spring two with bottom plate, and upper electromagnetism is nested with the top plate directly over micro-filtration pond
Iron two, position
Lower electromagnet two is nested with bottom plate immediately below the micro-filtration pond.
Micro-filtration is provided with microfiltration membranes in pond, and micro-filtrate entrance is provided with the pool wall in the micro-filtration pond above microfiltration membranes,
The micro-filtrate entrance is connected by flexible pipe with lysate outlet；It is provided with the pool wall in the micro-filtration pond below microfiltration membranes micro-
Filtrate (liquid.
Opening is provided with the top of UF membrane pond, separating liquid entrance is provided with the pool wall in UF membrane pond, the separating liquid enters
Mouth is connected by flexible pipe with micro-filtrate outlet.
Filtering cylinder is inserted with UF membrane pond, filtering cylinder filters from the opening insertion at the top of UF membrane pond
The casing wall of cylinder is slidably connected with the opening at the top of UF membrane pond for sealing, filters the height of cylinder and can lift.
The bottom of filtering cylinder is provided with seperation film, and the top exit for filtering cylinder passes through flexible pipe and small molecule collection vessel
It is connected.
The adsorption magnetic force piece by magnetic rotation is provided with the interior pool wall in the UF membrane pond below filtering cylinder, is inhaled
Interior rotating shaft is fixedly installed in the middle part of attached magnetic plate.
Rotation magnetic force piece is provided with the outer pool wall in UF membrane pond, the rotation magnetic force piece mutually adsorbs with adsorption magnetic force piece；Rotation
The middle part for turning magnetic plate is fixedly connected with outer shaft, and the motor that outer shaft can be on bottom plate be installed in is rotated by.
Pump and magnetic valve are provided with every flexible pipe.
The aperture of sample treatment film is more than the aperture of microfiltration membranes, and the aperture of microfiltration membranes is more than the aperture of seperation film.
The aperture of the microfiltration membranes is 1-10 μm, and the aperture of microfiltration membranes is 0.1-0.45 μm, point that seperation film can retain
Son amount is not less than 5KD.
Screw thread is laid with interior rotating shaft.
Sealing ring is provided between opening at the top of the casing wall of filtering cylinder and UF membrane pond.
Lower electromagnet one and lower electromagnet two are cyclic structure, and upper electromagnet one and upper electromagnet two are circular knot
Structure, the through hole that flexible pipe can be supplied to pass through is provided with upper electromagnet one.
After the utility model uses said structure, have the advantages that：
1. the setting of the pump and magnetic valve on above-mentioned flexible pipe and flexible pipe, on the one hand, can adapt to gravity environment and micro- heavy
The liquid conveying of force environment；On the other hand, periodic vibration can be carried out with cell sample processing pond and micro-filtration pond, is not influenceing to shake
While swinging, moreover it is possible to prevent pipeline blockage.
2. the setting of above-mentioned spring one, upper electromagnet one and lower electromagnet one so that cell sample processing pond can be carried out
Periodically shake, namely treat that cell lysis and lysate as cell sample processing pond carries out periodicity concussion, treat that cracking is thin
Born of the same parents grow with lysate time of contact, and are not easily blocked sample treatment film and flexible pipe, and lysis efficiency is high, and cleavage rate is high.On the other hand,
When in gravity environment, it is only necessary to the periodicity of electromagnet one is powered, the electromagnetic attraction of electromagnet one is more than carefully
The gravity in born of the same parents' sample treatment pond, so, you can cell sample processing pond is carried out periodicity concussion.When in microgravity environment
When, the upper periodicity alternate energisation of electromagnet one and lower electromagnet one, the electromagnetic attraction of lower electromagnet one that is, gravity environment,
Periodicity concussion is carried out to cell sample processing pond so as to realize.Furthermore it is possible to by realizing electrical current control to electromagnetism
The suction control of iron.
3. the setting of above-mentioned spring two, upper electromagnet two and lower electromagnet two, micro-filtration pond can be caused to carry out periodicity concussion,
Prevent microfiltration membranes and flexible pipe from blocking, micro-filtration pond periodically concussion principle is identical with cell sample processing pond.
4. the setting of above-mentioned filtering cylinder, on the one hand, filtering cylinder cylinder lift drive component in the presence of on glide
It is dynamic, it can prevent the macromolecules adsorption below seperation film from seperation film, preventing seperation film from blocking, macromolecular and small molecule point
From efficiency high.On the other hand, small molecule is directly carried out from seperation film by rear by filtering cylinder into small molecule collection vessel
Recovery, and the macromolecular in UF membrane pond is directly trapped in UF membrane pond, after the completion of separation of small molecuies, UF membrane pond
That is, macromolecular collection vessel, it is not necessary to macromolecular is carried out to capture again, reclaimed, simple to operate, macromolecular recovery effect
Rate is high.
Brief description of the drawings
Fig. 1 is a kind of structural representation of cell pretreatment device of the utility model.
Wherein have：
10. casing；
11. top plate；111. pending cell sample storage tank；112. buffer solution storage tank；113. lysate storage tank；
114. cylinder lifts drive component；Electromagnet one on 115.；Electromagnet two on 116.；
12. bottom plate；121. lower electromagnet one；122. lower electromagnet two；123. spring one；124. spring two；125. driving
Motor；126. outer shaft；127. rotary magnetic iron plate；
20. cell sample processing pond；21. cell sample entrance；22. buffer inlet；23. lysate entrance；24. sample
Process film；25. waste liquid exports；26. lysate exports；
30. micro-filtration pond；31. microfiltration membranes；32. micro-filtrate entrance；33. micro-filtrate exports；
40. UF membrane pond；41. filter cylinder；42. seperation film；Rotating shaft in 43.；431. adsorption magnet pieces；432. screw thread；
44. sealing ring；
50. waste liquid collection box；
60. flexible pipe；61. pump；62. magnetic valve；
70. small molecule collection vessel.
Embodiment
The utility model is described in further detail with specific better embodiment below in conjunction with the accompanying drawings.
As shown in figure 1, a kind of cell pretreatment device, including casing 10, cell sample processing pond 20, micro-filtration pond 30, film
Separate tank 40, waste liquid collection box 50, flexible pipe 60 and small molecule collection vessel 70.
Wherein, cell sample processing pond, micro-filtration pond, UF membrane pond, waste liquid collection box and small molecule collection vessel are all provided with
Put in casing.
Casing includes top plate 11, bottom plate 12 and side plate, wherein, top plate is connected by side plate with bottom plate.
Cell sample storage tank 111 to be handled, slow is set gradually on the top plate directly over cell sample processing pond
Fliud flushing storage tank 112 and lysate storage tank 113.
Pending cell sample storage tank 111, buffer solution storage tank 112 and lysate storage tank 113, it is preferably to be cylinder
Shape.
Top plate directly over cell sample processing pond, which embeds, is cased with upper electromagnet 1, preferably with through hole
It is circular.
Cell sample entrance 21, buffer inlet 22 and lysate entrance are disposed with the top of cell sample processing pond
23。
The outlet of pending cell sample storage tank enters with cell sample entrance, the outlet of buffer solution storage tank and buffer solution
Connected between mouth and the outlet of lysate storage tank and lysate entrance by flexible pipe 60.
Three flexible pipes being connected with cell sample entrance 21, buffer inlet 22 and lysate entrance 23 are respectively from upper electricity
Passed through in the through hole and top plate of magnet one.
Cell sample processing pond bottom is connected by spring 1 with bottom plate, immediately below cell sample processing pond
Bottom plate it is embedded be cased with lower electromagnet 1, lower electromagnet one is preferably cyclic structure, and spring one is located in ring cavity.
Sample treatment film 24 is provided with cell sample processing pond, the cell sample processing pond below sample treatment film
Pool wall on be respectively arranged with waste liquid outlet 25 and lysate outlet 26；Wherein, waste liquid outlet passes through flexible pipe and waste liquid
Collection box is connected.
The setting of above-mentioned spring one, upper electromagnet one and lower electromagnet one so that cell sample processing pond can be carried out week
Phase property is shaken, namely treats that cell lysis and lysate as cell sample processing pond carries out periodicity concussion, treat cell lysis
Grown with lysate time of contact, and be not easily blocked sample treatment film and flexible pipe, lysis efficiency is high, and cleavage rate is high.
When in gravity environment, it is only necessary to the periodicity of electromagnet one is powered, inhale the electromagnetism of electromagnet one
Power is more than the gravity of cell sample processing pond.So, when upper electromagnet one is powered, cell sample processing pond will be in electromagnetic attraction
In the presence of move up.After peak is moved in cell sample processing pond, upper electromagnet one powers off, cell sample processing pond
It will be moved in the presence of gravity and/or the pulling force of spring one under phase, when cell sample processing pond is displaced downwardly to minimum point, upper electricity
Magnet is powered again and again, goes round and begins again, you can cell sample processing pond is carried out periodicity concussion.
When in microgravity environment, the upper periodicity alternate energisation of electromagnet one and lower electromagnet one, lower electromagnet one
Electromagnetic attraction is that is, gravity environment.Upper electromagnet one is powered, and cell sample processing pond moves up, until peak, bullet
Spring one stretches, upper electromagnet one powers off, while lower electromagnet one is powered, and cell sample processing pond will provide in lower electromagnet one
In the presence of the power that automatically resets of electromagnetic attraction and spring one, move down, until minimum point, then, upper electromagnet one are logical
Electricity, go round and begin again, periodicity concussion is carried out to cell sample processing pond so as to realize.
Furthermore it is possible to by controlling electrical current the suction control realized to electromagnet.
Micro-filtration bottom of pond portion is connected by spring 2 124 with bottom plate, is nested with the top plate directly over micro-filtration pond
Electromagnet 2 116, lower electromagnet 2 122 is nested with the bottom plate immediately below micro-filtration pond.
Micro-filtration is provided with microfiltration membranes in pond, and micro-filtrate entrance is provided with the pool wall in the micro-filtration pond above microfiltration membranes
32, the micro-filtrate entrance is connected by flexible pipe with lysate outlet；Set on the pool wall in the micro-filtration pond below microfiltration membranes
There is micro-filtrate to export 33.
The setting of above-mentioned spring two, upper electromagnet two and lower electromagnet two, micro-filtration pond can be caused to carry out periodicity concussion, prevented
Only microfiltration membranes and flexible pipe block, and micro-filtration pond periodically concussion principle is identical with cell sample processing pond.
Opening is provided with the top of UF membrane pond, separating liquid entrance is provided with the pool wall in UF membrane pond, the separating liquid enters
Mouth is connected by flexible pipe with micro-filtrate outlet.
Filtering cylinder 41 is inserted with UF membrane pond, filtering cylinder inserts from the opening at the top of UF membrane pond, and mistake
The casing wall of air filtration cylinder is slidably connected with opening for sealing, filters the height of cylinder and can lift.
The highly preferred cylinder lifting drive component 114 by being arranged on top plate of filtering cylinder is driven.
The structure and principle of above-mentioned filtering cylinder are prior art, be will not be described in great detail here.
Sealing ring 44 is preferably provided between opening at the top of the casing wall of filtering cylinder and UF membrane pond.
The bottom of filtering cylinder is provided with seperation film 42, and the top exit for filtering cylinder collects appearance by flexible pipe and small molecule
Device is connected.
The adsorption magnetic force piece by magnetic rotation is provided with the interior pool wall in the UF membrane pond below filtering cylinder
431, interior rotating shaft 43 is fixedly installed in the middle part of adsorption magnetic force piece, and screw thread is preferably laid with interior rotating shaft.Under gravity environment, spiral shell
The liquid flow direction that line can be pointed in UF membrane pond is oriented to, and is lifted up the liquid positioned at separate tank bottom.
Rotation magnetic force piece 127 is provided with the outer pool wall in UF membrane pond, the rotation magnetic force piece mutually adsorbs with adsorption magnetic force piece；
The middle part of rotation magnetic force piece is fixedly connected with outer shaft 126, the driving for the motor 125 that outer shaft can be on bottom plate be installed in
Lower rotation.
Pump and magnetic valve are provided with every flexible pipe.
The aperture of sample treatment film is more than the aperture of microfiltration membranes, and the aperture of microfiltration membranes is more than the aperture of seperation film.
The aperture of the microfiltration membranes is preferably 1-10 μm, and the aperture of microfiltration membranes is preferably 0.1-0.45 μm, seperation film institute energy
The molecular weight of retention is preferably not less than 5KD.
Preferred embodiment of the present utility model described in detail above, still, the utility model is not limited to above-mentioned reality
The detail in mode is applied, in range of the technology design of the present utility model, the technical solution of the utility model can be entered
The a variety of equivalents of row, these equivalents belong to the scope of protection of the utility model.
Claims (5)
- A kind of 1. cell pretreatment device, it is characterised in that：Including casing, cell sample processing pond, micro-filtration pond, UF membrane pond, Waste liquid collection box, flexible pipe and small molecule collection vessel；Wherein, cell sample processing pond, micro-filtration pond, UF membrane pond, waste liquid Collection box and small molecule collection vessel are arranged in casing；Casing includes top plate, bottom plate and side plate, wherein, top plate is connected by side plate with bottom plate；Cell sample storage tank to be handled, buffer solution storage are set gradually on the top plate directly over cell sample processing pond Tank and lysate storage tank；Top plate directly over cell sample processing pond, which embeds, is cased with upper electromagnet one；Cell sample entrance, buffer inlet and lysate entrance are disposed with the top of cell sample processing pond；The outlet of pending cell sample storage tank and cell sample entrance, the outlet of buffer solution storage tank and buffer inlet, And connected between the outlet of lysate storage tank and lysate entrance by flexible pipe；Cell sample processing pond bottom is connected by spring one with bottom plate, in the bottom plate immediately below cell sample processing pond It is nested with lower electromagnet one；Sample treatment film is provided with cell sample processing pond, the pool wall of the cell sample processing pond below sample treatment film On be respectively arranged with waste liquid outlet and lysate outlet；Wherein, waste liquid outlet is connected by flexible pipe with waste liquid collection box Connect；Micro-filtration bottom of pond portion is connected by spring two with bottom plate, and upper electromagnet is nested with the top plate directly over micro-filtration pond Two, it is nested with lower electromagnet two on the bottom plate immediately below micro-filtration pond；Micro-filtration is provided with microfiltration membranes in pond, is provided with micro-filtrate entrance on the pool wall in the micro-filtration pond above microfiltration membranes, this is micro- Filtrate inlet is connected by flexible pipe with lysate outlet；Micro-filtrate is provided with the pool wall in the micro-filtration pond below microfiltration membranes Outlet；Opening is provided with the top of UF membrane pond, separating liquid entrance is provided with the pool wall in UF membrane pond, the separating liquid entrance leads to Flexible pipe is crossed with micro-filtrate outlet to be connected；Filtering cylinder is inserted with UF membrane pond, filtering cylinder filters cylinder from the opening insertion at the top of UF membrane pond Casing wall be slidably connected with the opening at the top of UF membrane pond for sealing, filtering the height of cylinder can lift；The bottom of filtering cylinder is provided with seperation film, and the top exit for filtering cylinder passes through flexible pipe and small molecule coated lycopene Connect；The adsorption magnetic force piece by magnetic rotation is provided with the interior pool wall in the UF membrane pond below filtering cylinder, adsorbs magnetic Interior rotating shaft is fixedly installed in the middle part of power piece；Rotation magnetic force piece is provided with the outer pool wall in UF membrane pond, the rotation magnetic force piece mutually adsorbs with adsorption magnetic force piece；Rotary magnetic The middle part of power piece is fixedly connected with outer shaft, and the motor that outer shaft can be on bottom plate be installed in is rotated by；Pump and magnetic valve are provided with every flexible pipe；The aperture of sample treatment film is more than the aperture of microfiltration membranes, and the aperture of microfiltration membranes is more than the aperture of seperation film.
- 2. cell pretreatment device according to claim 1, it is characterised in that：The aperture of the microfiltration membranes is 1-10 μm, The aperture of microfiltration membranes is 0.1-0.45 μm, and the molecular weight that seperation film can retain is not less than 5KD.
- 3. cell pretreatment device according to claim 1, it is characterised in that：Screw thread is laid with interior rotating shaft.
- 4. cell pretreatment device according to claim 1, it is characterised in that：Filter the casing wall and UF membrane Chi Ding of cylinder Sealing ring is provided between the opening in portion.
- 5. cell pretreatment device according to claim 1, it is characterised in that：Lower electromagnet one and lower electromagnet two are Cyclic structure, upper electromagnet one and upper electromagnet two are circular configuration, and being provided with upper electromagnet one can supply what flexible pipe passed through Through hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720939674.7U CN207031420U (en) | 2017-07-31 | 2017-07-31 | Cell pretreatment device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720939674.7U CN207031420U (en) | 2017-07-31 | 2017-07-31 | Cell pretreatment device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207031420U true CN207031420U (en) | 2018-02-23 |
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ID=61461713
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201720939674.7U Active CN207031420U (en) | 2017-07-31 | 2017-07-31 | Cell pretreatment device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108841510A (en) * | 2018-08-10 | 2018-11-20 | 遵义市永宏金属设备有限公司 | Processing cooling system for wine brewing |
| CN109456882A (en) * | 2019-01-16 | 2019-03-12 | 北京理工大学 | A kind of space biological sample micro process integrating device |
-
2017
- 2017-07-31 CN CN201720939674.7U patent/CN207031420U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108841510A (en) * | 2018-08-10 | 2018-11-20 | 遵义市永宏金属设备有限公司 | Processing cooling system for wine brewing |
| CN109456882A (en) * | 2019-01-16 | 2019-03-12 | 北京理工大学 | A kind of space biological sample micro process integrating device |
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