CN206974904U - A kind of automatic focusing device of CCD fluorescence biosensor chips scanner - Google Patents
A kind of automatic focusing device of CCD fluorescence biosensor chips scanner Download PDFInfo
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- CN206974904U CN206974904U CN201720796979.7U CN201720796979U CN206974904U CN 206974904 U CN206974904 U CN 206974904U CN 201720796979 U CN201720796979 U CN 201720796979U CN 206974904 U CN206974904 U CN 206974904U
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Abstract
It the utility model is related to a kind of automatic focusing device of CCD fluorescence biosensor chips scanner.Including light source, CCD camera, imaging lens, motor, single-chip microcomputer, the biochip that the light source is used to be opposite in front of imaging lens carries out fluorescence excitation, the fluoroscopic image of the imaged camera lens collection biochip of CCD camera, the motor is used for the focal length for adjusting imaging lens, and the light source, CCD camera, motor control by the single-chip microcomputer；The motor drives imaging lens to rotate by driving belt, to be implemented as the adjustment as lens focus.The utility model have it is simple in construction, imaging lens visual field size can be kept constant, and the advantages of the degree of accuracy is high, speed is fast and automaticity is high.
Description
Technical field
It the utility model is related to a kind of automatic focusing device of CCD fluorescence biosensor chips scanner.
Background technology
Patent（Application number：201220260024.7）A kind of automatic focusing system towards line array CCD is proposed, it is characterized in that
Pass through mobile camera lens（Change object distance）, and realize automatic focusing with the method for CCD collection images.Patent（Application number：
CN201610597471.4）It is proposed a kind of micro-array chip fluorescence detection method of great power LED, it is characterised in that pass through design
Annular LED illumination array biochip is excited, then using CCD camera gather transmitting fluorescence.Patent（Application
Number：CN02252864.4）A kind of design of the CCD biochip supplies analysis with uniform illumination system is proposed, passes through optics member
The white light that part sends xenon lamp is filtered into parallel monochromatic light, and its oblique is mapped on biochip then is excited into fluorescent dye,
The fluorescence of transmitting is captured with CCD camera again.
Biochip technology is the new and high technology to be emerged rapidly in life science, and it is with slide, silicon chip or Buddhist nun
Dragon etc. is carrier, arranges substantial amounts of biomaterial to high-density on its surface, is realized to DNA, protein, cell and other lifes
Accurate, quick, parallel and large information capacity the detection and analysis of thing component, can be widely applied to drug research, medical diagnosis on disease, base
Because of fields such as function research.The detection mode of the current main flow of fluoroscopic examination of biochip has two kinds：One kind is to be based on
The mode of the laser co-focusing of photomultiplier, another kind are the imagings of high pressure xenon gas lamp, mercury lamp or high-power LED combined CCD
Mode.Wherein, CCD imaging modes are reused by the way that light source is filtered into narrowband wavelength ranges and biochip is excited
CCD camera collects the fluorescence emitted, and which has the advantages of simple in construction, detection speed is fast.But CCD imaging modes
Have a problem that, that is, because the focal length of imaging lens is very sensitive, during mobile instrument or glass carrier bench disengaging
Camera lens is easily out of focus so that the image of shooting becomes fuzzy, and but also precision not only troublesome, time-consuming with the mode of manual focusing is held
Easily by people's subjective impact.Therefore, the present invention proposes a kind of automatic focusing design of CCD fluorescence biosensor chips scanner so that only
Simple operations instrument is needed with regard to automatic focusing process can be performed.
The content of the invention
The purpose of this utility model is to provide a kind of automatic focusing device of CCD fluorescence biosensor chips scanner, the dress
Put with it is simple in construction, imaging lens visual field size can be kept constant, and the degree of accuracy is high, speed is fast and automaticity is high
Advantage.
To achieve the above object, the technical solution of the utility model is：A kind of CCD fluorescence biosensor chips scanner it is automatic
Focus control, including light source, CCD camera, imaging lens, motor, single-chip microcomputer, the light source are used to be opposite in front of imaging lens
Biochip carry out fluorescence excitation, the fluoroscopic image of the imaged camera lens collection biochip of the CCD camera, the motor is used
In the focal length of adjustment imaging lens, the light source, CCD camera, motor control by the single-chip microcomputer.
In the embodiment of the utility model one, the motor drives imaging lens to rotate by driving belt, to be implemented as
As the adjustment of lens focus.
In the embodiment of the utility model one, the single-chip microcomputer is also associated with a host computer, to pass through PC control institute
CCD camera is stated, the fluoroscopic image of the biochip of the CCD camera collection is transferred to host computer and handled.
In the embodiment of the utility model one, communicated between the single-chip microcomputer and host computer by USB, it is described upper
Communicated between machine and CCD camera by USB.
Compared to prior art, the utility model has the advantages that：The utility model have it is simple in construction, can protect
It is constant to hold imaging lens visual field size, and the advantages of the degree of accuracy is high, speed is fast and automaticity is high.
Brief description of the drawings
Fig. 1 is the utility model device block diagram.
Fig. 2 is imaging schematic diagram.
Embodiment
Below in conjunction with the accompanying drawings, the technical solution of the utility model is specifically described.
A kind of automatic focusing device of CCD fluorescence biosensor chips scanner of the present utility model, including light source, CCD camera,
Imaging lens, motor, single-chip microcomputer, the biochip that the light source is used to be opposite in front of imaging lens carry out fluorescence excitation, institute
The fluoroscopic image of the imaged camera lens collection biochip of CCD camera is stated, the motor is used for the focal length for adjusting imaging lens, described
Light source, CCD camera, motor control by the single-chip microcomputer.The motor drives imaging lens to rotate by driving belt, with reality
It is ready-made as the adjustment of lens focus.
The single-chip microcomputer is also associated with a host computer, to be adopted by CCD camera described in PC control, the CCD camera
The fluoroscopic image of the biochip of collection is transferred to host computer and handled.Carried out between the single-chip microcomputer and host computer by USB
Communication, is communicated between the host computer and CCD camera by USB.
The realization principle of the utility model device is told about in detail below.
As shown in figure 1, the automatic focusing device of CCD fluorescence biosensor chips scanner of the present utility model, mainly by CCD phases
These parts of machine, host computer, imaging lens, motor, driving belt, single-chip microcomputer, light source, biochip form.Its is worked
Journey is as follows：Biochip is placed simultaneously using light source progress fluorescence excitation, by single-chip microcomputer controlled motor from the initial of lens focus
Position.Then since the initial position of lens focus, focalized in the adjustable extent of focal length using motor continuous coarse, every
Using CCD camera collection biological chip fluorescent picture at the end of secondary coarse adjustment, then image is transferred to the upper of PC by usb communication
Machine stores.Host computer is handled image after all fluoroscopic images of rough focusing are received, and judges image definition, then
Focal position as corresponding to single-chip microcomputer controlled motor by the Focussing of imaging lens to that maximum width image of definition, and
Repeat the process.
No matter more complicated imaging lens are, actually can be viewed as the ideal image system of single thin lens as shown in Figure 2
System model.Imaging system is imaged to point P, and u is object distance in figure, and v is image distance, when system is in quasi- coke-like state, the point in image planes
P ' is imaging system to object point P imagings.If f is the focal length of imaging system, then met according to Gaussian imaging equation, object image distance
Following relational expression：
When imaging parameters meet above-mentioned relation, imaging system is in quasi- coke-like state, and now imaging is most clearly.If
Imaging system is in out-of-focus appearance, and equally object point P is imaged, and now object image distance no longer meets Gaussian imaging equation, on detector
Imaging is no longer a point, but a disc of confusion.Focusing is exactly to adjust the parameter of imaging system, meets object image distance high
This imaging formula, obtains picture rich in detail.As shown from the above formula, focusing can be by changing object distance u, image distance v or focal length f
For realize, this is the basic principle of lens focusing.
Analysis based on more than for lens imaging system, the utility model are to utilize motor regulation imaging lens on hardware
The focal length f of head reaches the purpose of focusing.
Above is preferred embodiment of the present utility model, all changes made according to technical solutions of the utility model are produced
Function without departing from technical solutions of the utility model scope when, belong to the scope of protection of the utility model.
Claims (4)
- A kind of 1. automatic focusing device of CCD fluorescence biosensor chips scanner, it is characterised in that：Including light source, CCD camera, into As camera lens, motor, single-chip microcomputer, the biochip that the light source is used to be opposite in front of imaging lens carries out fluorescence excitation, described The fluoroscopic image of the imaged camera lens collection biochip of CCD camera, the motor are used for the focal length for adjusting imaging lens, the light Source, CCD camera, motor control by the single-chip microcomputer.
- A kind of 2. automatic focusing device of CCD fluorescence biosensor chips scanner according to claim 1, it is characterised in that： The motor drives imaging lens to rotate by driving belt, to be implemented as the adjustment as lens focus.
- A kind of 3. automatic focusing device of CCD fluorescence biosensor chips scanner according to claim 1, it is characterised in that： The single-chip microcomputer is also associated with a host computer, to pass through CCD camera described in PC control, the biology of the CCD camera collection The fluoroscopic image of chip is transferred to host computer and handled.
- A kind of 4. automatic focusing device of CCD fluorescence biosensor chips scanner according to claim 3, it is characterised in that： Communicated by USB between the single-chip microcomputer and host computer, communicated between the host computer and CCD camera by USB.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720796979.7U CN206974904U (en) | 2017-07-04 | 2017-07-04 | A kind of automatic focusing device of CCD fluorescence biosensor chips scanner |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201720796979.7U CN206974904U (en) | 2017-07-04 | 2017-07-04 | A kind of automatic focusing device of CCD fluorescence biosensor chips scanner |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113909680A (en) * | 2021-11-11 | 2022-01-11 | 中国科学院力学研究所 | Internal rotation laser head device |
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2017
- 2017-07-04 CN CN201720796979.7U patent/CN206974904U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113909680A (en) * | 2021-11-11 | 2022-01-11 | 中国科学院力学研究所 | Internal rotation laser head device |
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