CN206916154U - A kind of automatic cytological dimensional culture microcapsule generating apparatus - Google Patents

Abstract

The utility model discloses a kind of automatic cytological dimensional culture microcapsule generating apparatus, belong to biomedicine technical field.The automatic cytological dimensional culture microcapsule generating apparatus includes：Second housing is connected with the first housing；Touch-screen system is arranged on the first housing；Switch gate is flexibly connected with the opening of the second housing；Culture dish base is arranged on switch gate；Insulating base is arranged in second shell body；Jacking system is arranged in second shell body；Push rod system is arranged in first shell body；Injecting systems one end is connected with jacking system, and the other end is connected with the first housing；High voltage power supply is arranged in second shell body；Central controller is arranged in second shell body.The utility model automatic cytological dimensional culture microcapsule generating apparatus can produce micron-sized cell culture micro-capsule, beneficial to cell benign growths, ensure the homogeneity of cell culture and the accuracy of experiment.

Description

A kind of automatic cytological dimensional culture microcapsule generating apparatus

Technical field

Biomedicine technical field is the utility model is related to, more particularly to a kind of automatic cytological dimensional culture micro-capsule occurs Device.

Background technology

In the market Three-dimensional cell culture instrument cultivated mostly using cell adherence in suspended microspheres surface or Suspension culture is carried out in culture dish, although effect is more much better than traditional two-dimentional cell culture, actually attached cell Or internal layer cell has very big difference with okioplast local environment, each cell culture uniformity can not ensure；Meanwhile cultivate cell Microballoon yardstick it is general also bigger (grade), internal layer cell mass exchange is difficult, easily produces adverse reaction and death.

The content of the invention

The utility model provides a kind of automatic cytological dimensional culture microcapsule generating apparatus, solves or part solves now There are the attached cell of Three-dimensional cell culture instrument culture or internal layer cell in technology to have very big difference with okioplast local environment, Each cell culture uniformity can not ensure, it is general also bigger (grade) to cultivate the microballoon yardstick of cell, internal layer cellular material Exchange difficult, easy generation adverse reaction and dead technical problem.

In order to solve the above technical problems, the utility model provides a kind of automatic cytological dimensional culture microcapsule generating apparatus Including：First housing；Second housing, it is connected with first housing；Touch-screen system, it is arranged on first housing；Open Close the door, be flexibly connected with the opening of second housing；Culture dish base, it is arranged on the switch gate, the culture dish Culture dish is provided with base；Insulating base, it is arranged in the second shell body, negative plate is enclosed with the insulating base； Jacking system, it is arranged in the second shell body；Push rod system, it is arranged in the first shell body；Injecting systems, one end and institute Jacking system connection is stated, the other end is connected with first housing, the injecting systems other end described in the push rod systems force, when When the switch gate is closed, second housings close, the culture dish base is arranged on above the negative plate, the injection One end of system is placed in above the culture dish；High voltage power supply, it is arranged in the second shell body, the positive pole of the high voltage power supply It is connected with the jacking system, the negative pole of the high voltage power supply is connected with the negative plate；Central controller, it is arranged on described In two housings, the central controller is connected with the touch-screen system, jacking system and push rod system；Wherein, the center Controller receives the touch-screen system and sends control signal, is sent according to control signal to the jacking system and push rod system Action signal.

Further, USB interface is provided with first housing, the USB interface is connected with the central controller, Set on first housing and syringe cover plate is movably set with the injecting systems, note is provided with the syringe cover plate Emitter observation window.

Further, heat emission hole is offered on second housing, partition wall, the partition wall are provided with the second shell body Second housing is divided into the first accommodation space and the second accommodation space, it is accommodating that the jacking system is arranged on described first In space, the high voltage power supply and central controller are arranged in second accommodation space.

Further, culture dish observation window and indent handle are offered on the switch gate.

Further, the touch-screen system includes：Touch-screen mounting bracket, rotating shaft installing plate, touch-screen and touch-screen Protecting crust；Described touch-screen mounting bracket one end is provided with spanner boss；Described rotating shaft installing plate one end by Damping rotation shaft with The touch-screen mounting bracket other end connection, the other end are connected with first housing；The touch-screen is arranged on described touch Touch in screen mounting bracket, the touch-screen is connected with the central controller, and control signal is sent to the central controller；Institute Touch-screen protecting crust is stated to be connected with the touch-screen mounting bracket.

Further, the jacking system includes：Lift mounting bracket, lifting motor, syringe needle driving arm, slip Card, insulation connecting plate, syringe needle card, woven hose clip, knocker female end and microswitch；Mounting bracket is lifted, is arranged on described In second shell body, notch is offered in the lifting mounting bracket, scale is provided with the lifting mounting bracket；Lifting driving Motor, it is arranged in the lifting mounting bracket, the lifting motor is connected with the central controller, during receiving is described The action signal for entreating controller to send；Syringe needle driving arm, it is connected through the notch with the lifting motor；Slide card Piece, it is connected with the syringe needle driving arm, the slip card is consistent with the notch position；Insulate connecting plate, one end It is connected with the syringe needle driving arm；Syringe needle card, it is connected with the insulation connecting plate other end, the syringe needle card and the high pressure The positive pole connection of power supply, the syringe needle card are connected with the injecting systems；Woven hose clip, it is arranged on the insulation connecting plate On；Knocker female end, it is arranged in the lifting mounting bracket, the sub- end of knocker is provided with the switch gate, when the switch gate During closing, the knocker female end is flexibly connected with the sub- end of the knocker；Microswitch, it is arranged in the lifting mounting bracket, Microswitch boss is provided with the switch gate, when the switch gate is closed, the microswitch of stating is opened with the fine motion Close boss contact, the microswitch closure.

Further, the push rod system includes：Propel fixed support, propel motor, injector push-rod, push rod cap And proximity transducer；The fixed support of propelling is arranged in first shell body；The motor of propelling is arranged on described propel On fixed support, the motor of propelling is connected with the central controller, receives the action that the central controller is sent Signal；The injector push-rod is connected with the motor of propelling；The push rod cap is arranged on the injector push-rod, institute State one end that push rod cap promotes the injecting systems；The proximity transducer is arranged on the push rod cap, described close to sensing Device is connected with the central controller, and position feed back signal is sent to the central controller.

Further, the injecting systems include：Syringe mounting blocks, syringe, woven hose and draw point；The syringe Mounting blocks are connected with first housing, and the position of the position of the syringe mounting blocks and the syringe cover plate is the same as always On line；The syringe is arranged on the syringe mounting blocks；Described woven hose one end connects with the syringe；The steel Pin is connected with the woven hose other end, and the draw point is arranged on the jacking system.

Further, automatic cytological dimensional culture microcapsule generating apparatus of the present invention also includes：Disinfection system, it is arranged on institute State in the first accommodation space.

Further, the disinfection system includes：Uviol lamp, it is arranged in first accommodation space.

The one or more technical schemes provided in the embodiment of the present application, have at least the following technical effects or advantages：

Due to switch gate being closed, for switch gate by the second housings close, culture dish base is arranged on insulating base parcel Above negative plate, one end of injecting systems is placed in above the culture dish, and central controller receives to be arranged on the first housing The control signal that touch-screen system is sent, according to control signal to jacking system and push rod system sending action signal, by certainly Dynamicization controls, and eliminates manual operation error, each micro-capsule size can keep good uniformity to ensure cell culture Homogeneity and the accuracy of experiment, and then injecting systems one end is connected by jacking system with high-voltage power cathode, negative plate It is connected with high voltage power supply negative pole, a high-voltage electrostatic field is formed between one end of injecting systems and culture dish, by injecting systems Load the suspension of cell and bioactive materials, one end of push rod systems force injecting systems injects suspension into culture dish Liquid, with the presence of big quantity of material with ionic state in suspension, while material further ionizes in high voltage electric field in suspension, and Suspension intermediate ion orients in the electric field to be gathered, so as to which high-voltage electrostatic field is to the small liquid of suspension at the syringe needle of the draw point of injecting systems Drop produces pulling force effect, when droplet overcomes droplet surface tension and viscosity resistance under gravity and electric field force effect, drop Fall in culture dish, particular gel salt bath solution is housed in culture dish, droplet forms thin one by one with salt bath solution crosslinking Born of the same parents cultivate micro-capsule, and cell culture micro-capsule size is up to micron order, and internal layer cell mass exchange is easy, beneficial to cell benign growths.

Brief description of the drawings

Fig. 1 is the structural representation for the automatic cytological dimensional culture microcapsule generating apparatus that the utility model embodiment provides Figure；

Fig. 2 is the internal structure schematic diagram of Fig. 1 automatic cytological dimensional culture microcapsule generating apparatus；

Fig. 3 is the structural representation of the second housing of Fig. 1 automatic cytological dimensional culture microcapsule generating apparatus；

Fig. 4 is the side view of Fig. 3 the second housing；

Fig. 5 is the structural representation of the touch-screen system of Fig. 1 automatic cytological dimensional culture microcapsule generating apparatus；

Fig. 6 is the structural representation of the jacking system of Fig. 1 automatic cytological dimensional culture microcapsule generating apparatus；

Fig. 7 is the structural representation of the push rod system of Fig. 1 automatic cytological dimensional culture microcapsule generating apparatus；

Fig. 8 is the structural representation of the injecting systems of Fig. 1 automatic cytological dimensional culture microcapsule generating apparatus

Fig. 9 is the structure of another embodiment of the culture systems of Fig. 1 automatic cytological dimensional culture microcapsule generating apparatus Schematic diagram.

Embodiment

Referring to Fig. 1-4, a kind of automatic cytological dimensional culture microcapsule generating apparatus bag of the utility model embodiment offer Include：Automatic cytological dimensional culture microcapsule generating apparatus includes：First housing 2, the second housing 3, touch-screen system 1, switch gate 4th, culture dish base 10, insulating base 11, negative plate 12, jacking system 13, push rod system 14, injector system 15, center control Device 17 processed and high voltage power supply 18.

Second housing 3 is connected with first housing 2.

The touch-screen system 1 is arranged on first housing 2.

The switch gate 4 is flexibly connected with the opening of second housing 3.

The culture dish base 10 is arranged on the switch gate 4, and culture dish 9 is provided with the culture dish base 10.

The insulating base 11 is arranged in second housing 3, and negative plate 12 is enclosed with the insulating base 11.

The jacking system 13 is arranged in second housing 3.

The push rod system 14 is arranged in first housing 2.

Described one end of injecting systems 15 is connected with the jacking system 13, and the other end is connected with first housing 2, described Push rod system 14 promotes the injecting systems other end, and when the switch gate 4 is closed, second housing 3 is closed, the training Support ware base 10 and be arranged on the top of negative plate 12, one end of the injecting systems 15 is placed in the top of culture dish 9.

The high voltage power supply 18 is arranged in second housing 3, and the positive pole of the high voltage power supply 18 is with the lifting System connection, the negative pole of the high voltage power supply 18 are connected with the negative plate.

The central controller 17 is arranged in second housing 3, the central controller 17 and the touch-screen system System 1, jacking system 13 and push rod system 14 connect.

Wherein, the central controller 17 receives the touch-screen system 1 and sends control signal, according to control signal to institute State jacking system 13 and the sending action signal of push rod system 14.

Technical scheme is due to switch gate 4 being closed, and switch gate 4 closes the second housing 3, and culture dish base 10 is set Put above the negative plate 12 wrapped up in insulating base 11, one end alignment culture dish 9 of injecting systems 15, central controller 17 receives The control signal that touch-screen system 1 on the first housing 2 is sent is arranged on, according to control signal to jacking system 13 and push rod system Unite 14 sending action signals, by Automated condtrol, eliminate manual operation error, each micro-capsule size can keep good one The one end of injecting systems 15 is passed through jacking system 13 by cause property so as to ensure the accuracy of the homogeneity of cell culture and experiment It is connected with the positive pole of high voltage power supply 18, negative plate 12 is connected with the negative pole of high voltage power supply 18, in one end of injecting systems 15 and culture dish 9 Between form a high-voltage electrostatic field, the suspension of cell and bioactive materials, push rod system 14 will be loaded in injecting systems 15 One end of injecting systems 15 injection suspension into culture dish 9 is promoted, with the presence of big quantity of material with ionic state in suspension, simultaneously Material further ionizes in high voltage electric field in suspension, and suspension intermediate ion orients gather in the electric field, so as to high pressure Electrostatic field produces pulling force effect to suspension droplet at the syringe needle of the draw point of injecting systems 13, when droplet is in gravity and electric field When overcoming droplet surface tension and viscosity resistance under power effect, it is dropped in culture dish 9, equipped with specific solidifying in culture dish 9 Glue salt bath solution, droplet form cell culture micro-capsule one by one with salt bath solution crosslinking, and cell culture micro-capsule size is up to micro- Meter level, internal layer cell mass exchange is easy, beneficial to cell benign growths.

The structure of the first housing 2 and the second housing 3 is discussed in detail.

Usb 19 is provided with first housing 2, the usb 19 is connected with the central controller 17, is used Data are sent in the data for receiving the central controller 17 or to the central controller 17.Set on first housing 2 Put and be movably set with syringe cover plate 6 at the injecting systems 15.The syringe cover plate 6 is arranged on first housing 2 Front end, face syringe 15-2 installation site, the syringe cover plate 6 are used to avoid in experimentation experimenter to described Syringe 15-2 false touch is moved or the interference of other extraneous factors.Due to needing frequently to change the syringe 15- in experimentation 2, therefore syringe cover plate 6 is designed as rotatable state, specifically, in the present embodiment, the syringe cover plate 2-1 is by turning Axle construction is flexibly connected with first housing 2, and can realize that opening is kept automatically, to facilitate experiment operator to institute State syringe 15-2 installation and replacing.Meanwhile syringe observation window 7 is provided with the syringe cover plate 6, note described in face Emitter 15-2, can allow the injection shape of experiment operator mixed liquor in syringe 15-2 described in Real Time Observation in experimentation State.The front end of second housing 2 is also provided with the break-make that power switch 8 is used for control device power supply；It is arranged on the second housing 2 Front end, facilitate experimenter to operate.

Heat emission hole 3-2 is offered on second housing 3, heat emission hole 7-4 is offered on second housing 3, to ensure The work radiating requirements of high voltage power supply 18, central controller 17 and other electrical equipment.Partition wall 3-1 is provided with second housing 3, Second housing 3 is divided into the first accommodation space and the second accommodation space by the partition wall, and the jacking system 13 is arranged on In first accommodation space, the high voltage power supply 18 and central controller 17 are arranged in second accommodation space, described Partition wall 3-1 effect one is to increase the intensity of the second housing 3, and it is that the inner space of lower house 3 is divided into two parts that it, which acts on two, the One accommodation space is as various parts assembly spaces, for installing the parts such as central processing unit 17, high voltage power supply 18, second Accommodation space and the switch gate 4 form a closed area, as experimental implementation region.

The structure of touch-screen system 1 is discussed in detail.

Referring to Fig. 5, the touch-screen system include touch-screen mounting bracket 1-1, rotating shaft installing plate 1-4, touch-screen 1-2 and Touch-screen protecting crust 1-3.

Described touch-screen mounting bracket 1-1 one end is fixedly installed spanner boss 1-1-1, and is set in first housing 1 Put and otch offered at the spanner boss 1-1-1, ensure experimenter can one hand by the spanner boss 1-1-1 institute Touch-screen mounting bracket 1-1 pull-ups are stated, the spanner boss 9-1-1 can also ensure that experimenter is convenient and the touch-screen is installed Support 1-1 rotates to other positions by initial position.

Described rotating shaft installing plate 1-4 one end passes through Damping rotation shaft 1-5 and touch-screen mounting bracket 1-1 other ends activity Connection, the other end are fixedly connected by bolt with first housing 1.The Damping rotation shaft 1-5 can realize the touch-screen The rotation of mounting bracket 1-1 optional positions stops.

The touch-screen 1-2 is fixedly installed in the touch-screen mounting bracket 1-1, the touch-screen 1-2 with it is described in Centre controller 17 is connected, and control signal is sent to the central controller 17.Wherein, the touch-screen mounting bracket 1-1 is tactile Screen 1-2 assembling matrixes are touched, while are also touch-screen 1-2 supporting plate and protective stent, to ensure that touch-screen 1-2 systems are rotating During without damage.

The touch-screen protecting crust 1-3 is fixedly connected with the touch-screen mounting bracket 1-1, the touch-screen protecting crust 1- 3 couples of touch-screen 1-2 are protected, while are also ensured attractive in appearance.

The structure of switch gate 4 is discussed in detail.

Indent handle 4-1 is provided with the switch gate 4, facilitates operator to open or close switch gate 4, while ensures to open 4 front plans close the door without protrusion, facilitates packaging and the transhipment of device, can also ensure appearance looks elegant.

Culture dish observation window 5 is provided with the switch gate 4, the culture dish observation window 5 is arranged on the interposition of switch gate 4 Put, culture dish 9 in corresponding experimental work region, for experimental implementation person, real time inspection three-dimensional cell cultivation is micro- in experimentation Capsule generating state.

The structure of culture dish base 10 and culture dish 9 is discussed in detail.

The culture dish base 10 is fixedly connected with the switch gate 4.The culture dish base 10 can be beaten with switch gate 4 Open and depart from Experimental Area, facilitate experiment operator to pick and place the culture dish 9.It is it should be noted that real in device to ensure Working region bactericidal effect is tested, culture dish base 10 is designed as pellucidity.

The culture dish 9 is arranged on the culture dish base 10.Specifically, in the present embodiment, the culture dish Corresponding neck is offered on base 10, it is ensured that the culture dish 9 is placed stable.It is filled with the culture dish 9 specific Gel salt bath solution, for accepting the bioactive materials of the drippage of injecting systems 15 and the suspension drop of cell to be cultivated, this is mixed Suspension drips to react to form three-dimensional cell cultivation micro-capsule one by one with gel salt bath solution crosslinking.Experimental needs, the training Support ware 9 and different specifications can be selected, therefore plurality of specifications culture dish neck is also offered on culture dish base 10, to meet various realities Test demand.

Referring to Fig. 9, based on identical inventive concept, the present invention also provides a kind of culture dish 9 and the culture dish bottom The set-up mode of seat 10.The culture dish 9 is arranged on the culture dish base 10, and the culture dish base 10 may be designed as taking out Pull-up structure, it is integrated in by guide rail on insulating base 11.

The structure of negative plate 12 and insulating base 11 is discussed in detail.

The negative plate 12 is connected with the negative pole of the high voltage power supply 12, the shape when testing progress and in jacking system 13 Into high-voltage electrostatic field.

The insulating base 11 is fixedly installed in second housing 3, and the insulating base 11 wraps up the negative plate 12, the insulating base 11 is placed in experimental work region centre position.The negative plate 12 is offered on the insulating base 11 Otch is installed, after both coordinate, the insulating base 11 wraps the negative plate 12, may be caused for taking precautions against high-tension circuit Harm.

When the switch gate 6 is closed, the culture dish 9 is arranged on the top of the negative plate 12.

The structure of jacking system 13 is discussed in detail.

Referring to Fig. 6, the jacking system includes：Lift mounting bracket 13-2, lifting motor 13-1, slide card 13-3, syringe needle driving arm 13-4, insulation connecting plate 13-5, syringe needle card 13-7, woven hose clip 13-6, knocker female end 13-10 And microswitch 13-9.

The lifting mounting bracket 13-2 is fixedly installed in second housing 3, on the lifting mounting bracket 13-2 Offer notch, scale be provided with the lifting mounting bracket 13-2, for identify injecting systems 15 and negative plate 12 away from From facilitating experiment operator macroscopic view to understand experimental state and regulation experiment parameter.The notch is guarantee syringe needle driving arm 13-4 is connected through lifting mounting bracket 13-2 with lifting motor 13-1, and ensures to move up and down.

The lifting motor 13-1 is fixedly installed on the lifting mounting bracket 13-2, the lifting motor 13-1 is connected with the central controller 17, receives the action signal that the central controller 17 is sent.The lifting driving electricity Machine 13-1 can use stepper motor or servomotor with position detecting function, on lifting mounting bracket 13-2, and For driving syringe needle driving arm 13-4 to move up and down.

The syringe needle driving arm 13-4 is fixedly connected through the notch with the lifting motor 13-1.

The slip card 13-3 is fixedly connected with the syringe needle driving arm 13-4, the slip card 13-3 with it is described Notch position is consistent, and is ensured in the syringe needle driving arm 13-4 during up and down motion, the lifting mounting bracket Notch on 13-2 ensure that the bactericidal effect in experimental work region all in sealing state.

Described insulation connecting plate 13-5 one end is fixedly connected with the syringe needle driving arm 13-4, due to syringe needle Ka13-7Chu In high-tension circuit, the insulation connecting plate 13-5 can prevent influence of the high-tension circuit to other parts.The insulation connecting plate 13-5 is using the good material manufacture of insulating properties.

The syringe needle card 13-7 is connected with the insulation connecting plate 13-5 other ends, the syringe needle card 13-7 and the high pressure The positive pole connection of power supply 18.The syringe needle card 13-7 is connected with the injecting systems 15.Specifically, in the present embodiment, institute State injecting systems 2 to be fastened on the syringe needle card 3-7 by hand-tighten bolts 13-8, facilitate the installation and more of the injecting systems 2 Change.

The woven hose clip 13-6 is arranged on the insulation connecting plate 13-5, for fixing the transfusion of injecting systems 15 Pipe 15-3, the woven hose clip 13-6 are designed using opening, and the woven hose 15-3 of the injecting systems 15 can be installed conveniently And dismounting.

The knocker female end 13-10 is fixedly installed on the lifting mounting bracket 13-2, is provided with the switch gate 4 The sub- end of knocker, when the switch gate 4 is closed, the knocker female end 13-10 is flexibly connected with the sub- end of the knocker, is easy to described The opening and closing of switch gate 4, and realize the closing hold mode of the switch gate 4.

The microswitch 3-9 is fixedly installed on the lifting mounting bracket 3-2, is provided with the switch gate 4 micro- Dynamic switch boss 4-2, when the switch gate 6 is closed, the microswitch 3-9 contacts with the microswitch boss 4-2, when When the switch gate 6 is opened, the microswitch 3-9 and microswitch boss 4-2 departs from, and microswitch 13-9 disconnects, Circuit disconnects, and microswitch 13-9 coordinates with the microswitch boss 4-2 on switch gate 4, protects experiment operator, realizes Enabling defencive function.

The jacking system 13 is arranged on the left of experimental work region, is mainly used in controlling draw point 15-4 and negative plate 12 Distance, so as to adjust the power of high-voltage electrostatic field.

The structure of push rod system 14 is discussed in detail.

Referring to Fig. 7, the push rod system includes：Propel fixed support 14-2, propel motor 14-1, injector push-rod 14-3, push rod cap 14-4 and proximity transducer 14-5.

The fixed support 14-2 that propels is fixedly installed in the first housing 2.The fixed support 13-2 that propels is as pushing away The assembling matrix of lever system 14, push rod system 14 is fixed on for other parts in installation system, and by fastening bolt The front end of second housing 2.

It is described propel motor 14-1 be fixedly installed on it is described propel on fixed support 14-2, it is described to propel motor 14-1 is connected with the central controller 17, receives the action signal that the central controller 17 is sent.It is described to propel driving electricity Driving power sources of the machine 14-1 as push rod system 14, stepper motor or servomotor with position detecting function can be used, For driving injector push-rod 14-3 linear motion.

The injector push-rod 14-3 is fixedly connected with the motor 14-1 that propels, and promotes the note of injecting systems 15 Emitter 15-2 pushrod movement, carry out the injection of mixed liquor.

The push rod cap 14-4 is fixedly installed on the injector push-rod 14-3, and the push rod cap 14-4 promotes the note One end of system 2 is penetrated, injector push-rod 8-3 and the flexible contact of injecting systems 2 are ensured by the push rod cap 14-4.The push rod Cap 14-4 is made of micro-elasticity material, such as elastomeric material.

The proximity transducer 14-5 is fixedly installed on the push rod cap 14-4, the proximity transducer 14-5 with it is described Central controller 17 is connected, and position feed back signal is sent to the central controller 17.The proximity transducer 14-5 is used to examine Survey the push rod cap 14-4 and the injecting systems 2 contact condition；When the push rod cap 14-5 contacts with injecting systems 15, The proximity transducer 14-5 controls to the feedback signal of central controller 17, the central controller 17 according to feedback signal It is described to propel motor 14-1 adjustment operational factors, the experiment set using more accurate parameters.

The push rod system 14 is installed on the front end of the first housing 2, is mainly used in automatically controlling the biology of injecting systems 15 The injection work of the suspension of active material and cell to be cultivated.

The structure of injecting systems 15 is discussed in detail.

Referring to Fig. 8, the injecting systems include：Syringe cover plate 15-1, syringe mounting blocks 15-3 and syringe 15-4, Woven hose 15-5 and draw point 15-6.

The syringe mounting blocks 15-3 is fixedly connected with first housing 2, the position of the syringe mounting blocks 15-3 Put with the position of the syringe cover plate 6 on the same line.The syringe mounting blocks 15-3 is designed as detachable pattern, and Plurality of specifications is designed as, experiment operator can be matched as needed.There is respective card slot on the syringe mounting blocks 15-3, use In the installation syringe 15-4.It should be noted that one to three difference can be set on the syringe mounting blocks 15-3 Neck, for installing the syringe 15-4 of different size, widen the free degree of experimental implementation.

The syringe 15-4 is arranged on the syringe mounting blocks 15-3.

Described woven hose 15-5 one end connects with the syringe 15-4.

The draw point 15-6 connects with the woven hose 15-5 other ends, and the draw point 15-6 is arranged on the jacking system On 13, for producing suspension droplet.The draw point 15-6 is stainless pin.

Specifically, because experiment needs to carry out in confined space, while it is also contemplated that in injecting systems 15 and tests consumptive material The convenience that (syringe 15-4, woven hose 15-5, draw point 15-6) is installed and changed, the first housing 2 and the corresponding positions of the second housing 3 Put offer woven hose 15-5 by neck, by the neck, woven hose 15-5 can be by extending outside experimental work region To experimental work region, while the neck can ensure woven hose 15-5 convenient installation and change, and be closed in switch gate 4 After conjunction, experimental work region can remain a confined space.

The injector system 15 is also installed on the front end of the first housing 2, is mainly used in installation and the mixed liquor of syringe Distribution.

Automatic cytological dimensional culture microcapsule generating apparatus of the present invention also includes：Disinfection system.

The disinfection system is fixedly installed in second housing 3.

The disinfection system includes：Uviol lamp 16.

The uviol lamp 16 is fixedly installed in second housing 3, preceding to experimental work region for being carried out in experiment Sterilization processing is carried out, to ensure that experiment is smoothed out.

Wherein, the central controller 17 in the present invention automatically controls maincenter as device, is installed on the second shell Second accommodation space of body 3, for receiving experimental implementation instruction and various feedback informations, and control device automatically completes experiment Operation.High voltage power supply 18 is also installed on the second accommodation space of the second housing 3, for producing high-voltage electrostatic field.High voltage power supply 18 Electromagnetic radiation protection measure need to be done, and enough distances are kept with other parts.

The utility model embodiment is introduced in order to clearer, is given below from the application method of the utility model embodiment To introduce.

First housing 2 and the second housing 3 are combined together by falcon slot structure and fastening bolt, are installed for other parts Assembling matrix is provided, and forms Internal Experiment space.The middle part of second housing 3 is designed with midfeather 3-1, and it is increase that it, which acts on one, The intensity of second housing 3, it is that the inner space of the second housing 3 is divided into first the second accommodation space of accommodation space level that it, which acts on two, Second accommodation space installs central processing unit 17 and high voltage power supply 18, and high voltage power supply 18 is used to produce high-voltage electrostatic field.High-tension electricity Source 18 need to do electromagnetic radiation protection measure.First accommodation space and switch gate 4 form a closed area, as experimental implementation area Domain.

Touch-screen system 1 by rotating shaft installing plate 1-4 be arranged on the first housing 2 upper center inclined-plane on, as with reality Testing the communication interface of personnel, experimenter can carry out the input of operational order by touch-screen 1-2, and observation experiment process Real-time status.The original state of touch-screen system 1 is parallel with the inclined-plane of the first housing 2, so that experiment operator is easier to touching Screen system 1 carries out experimental implementation and observation.General Biochemistry Experiment need to be carried out in toilet or superclean bench, when super Because superclean bench space is limited, and it is enclosed construction, although touch-screen system 1 is initial when being tested in net workbench State is arranged at an angle, but experimenter is still not easy to experimental implementation and observation.Rotating shaft installing plate 1-4 one end passes through resistance Buddhist nun's rotating shaft 1-5 is flexibly connected with the touch-screen mounting bracket 1-1 other ends, and the other end is fixedly connected with the first housing 1, Damping rotation shaft 1-5 can realize that the rotation of touch-screen mounting bracket 1-1 optional positions stops, and experimenter can be according to experiment actual conditions tactile Touch screen system 1 from initial position rotate to vertical position between any angle, so as to experimental implementation and state observation, therefore touch Screen system 9 is rotary structure, and is positively retained at optional position state.According to lab space demand, operating personnel pass through one hand Touch-screen mounting bracket 1-1 pull-ups, spanner boss 1-1-1 can also be ensured that experimenter facilitates handle by operation spanner boss 1-1-1 Touch-screen mounting bracket 1-1 rotates to other positions by initial position, and touch-screen 1-2 is fixedly installed on touch-screen mounting bracket 1- In 1, touch-screen 1-2 is connected with central controller 17, to central controller 11 send control signal, touch-screen protecting crust 1-3 with Touch-screen mounting bracket 1-1 is fixedly connected, and touch-screen 1-2 is protected, while is also ensured attractive in appearance.

During original state, jacking system 13 is in initial zero position, and push rod system 14 is also at initial zero position, and switch gate 4 closes Close, syringe cover plate 6 is closed.When needing to be tested, experiment operator opens syringe cover plate 6, opens switch gate 4, A kind of selected gauge hypodermic device 15-4 sucks the suspension of a certain amount of cell to be cultivated and bioactive materials, is installed on syringe On mounting blocks 15-3, and connect woven hose 15-5 and (can also first connect woven hose 15-5, then be installed to syringe mounting blocks again On 15-3).Then woven hose 15-5 is allowed to extend to working region by neck accordingly by the first housing 2 and the second housing 3, Then stainless pin 15-6 is installed on syringe needle card 15-7, fastened by hand-tighten bolts 15-8.It is a certain amount of specific solidifying taking up The culture dish 9 of glue salt bath solution is positioned on culture dish base 10；Switch gate 4 is then shut off, closes syringe cover plate 6.Will be exhausted Edge bottom plate 11 is arranged in the first accommodation space, and negative plate 12 is arranged on insulating base 11, the negative pole of high voltage power supply 18 with Negative plate 12 connects, and insulating base 11 wraps negative plate 12, harm caused by for taking precautions against high-tension circuit possibility, high voltage power supply 18 positive pole is connected with syringe needle card 13-7.The axis mechanism that rotates of switch gate 4 rotates in the first accommodation space opening, until knocker End is flexibly connected with knocker female end 13-10, and microswitch boss 4-2 contacts with microswitch 13-9.Due to culture dish base 10 Be connected with switch gate 4, culture dish 9 is arranged in culture dish base 10, switch gate 4 close after, culture dish 9 negative plate 12 just on Side.

8 are turned on the power switch, system energization.Central controller 17 controls uviol lamp 16 to kill virus experimental work region Sterilization treatment.By in touch-screen 1-2 man-machine interface input experiment parameter (as experiment specification of syringe, experimental voltage, experiment away from From, injection speed, volume injected etc.), control signal is sent to central controller 11 by touch-screen 1-2, central controller to Motor 13-1 sending action signals are lifted, lifting motor 13-1 drives syringe needle driving arm 13-4 actions.To ensure The bactericidal effect in experimental work region, it is provided with syringe needle driving arm 13-4 and slides card 13-3, face lifting mounting bracket Notch on 13-2, and be fitted close with lifting mounting bracket 13-2, ensure moving up and down in syringe needle driving arm 13-4 Notch on Cheng Zhong, lifting mounting bracket 13-2 is all in sealing state.Syringe needle driving arm 13-4 passes through the connecting plate 13- that insulate 5 drive syringe needle card 13-7 actions, and syringe needle 15-6 is fastened on syringe needle card 13-7 by hand-tighten bolts 13-8, in insulation connecting plate Woven hose clip 13-6 is installed, for fixing woven hose 15-5 on 13-5.Woven hose 15-5 one end connects with syringe needle 15-6, separately One end connects with syringe 15-4.Central controller 17 propels motor to motor 14-1 sending action signals are propelled 14-1 drives push rod cap 14-4 actions by injector push-rod 14-3, is transported by push rod cap 14-3 pushing syringes 15-4 push rod It is dynamic, the injection of mixed liquor is carried out, ensure that injector push-rod 14-3 and syringe 15-4 flexible contacts, push rod cap 14-4 is using micro- Elastomeric material is made, such as elastomeric material.Proximity transducer 14-5 is designed with push rod cap 14-4, for detecting push rod cap 14-4 With syringe 15-4 contact condition；When push rod cap 14-4 contacts with syringe 15-4, proximity transducer 14-5 is controlled to center The feedback signal of device 17 processed, central controller 17 control push rod system 14 to adjust operational factor according to feedback signal, and use is more accurate The experiment that parameter is set.Syringe needle 15-6 is directed at culture dish 9.

Push rod cap 14-4 pushing syringes 15-4 injection suspensions into culture dish 9, because culture dish 9 is arranged on negative plate On 12, the negative pole of high voltage power supply 18 is connected with negative plate 12, and positive pole is connected with syringe needle card 14-7, and syringe needle 15-6 is arranged on syringe needle card On 14-7, a high-voltage electrostatic field is formed between syringe needle 15-6 and culture dish 9, has big quantity of material to be deposited with ionic state in suspension , while material further ionizes in high voltage electric field in suspension, and suspension intermediate ion orients gather in the electric field, from And high-voltage electrostatic field produces pulling force effect to suspension droplet at stainless steel syringe needle, when droplet acts in gravity and electric field force Under when overcoming droplet surface tension and viscosity resistance, be dropped in the culture dish 9 of lower section, particular gel salt be housed in culture dish 9 Solution is bathed, droplet forms cell culture micro-capsule one by one with salt bath solution crosslinking, cell culture micro-capsule size can be made reachable Micron order, cell can carry out sufficient mass exchange with nutrient solution in micro-capsule, beneficial to cell benign growths, because yardstick is small, Cell individual eliminates the difference come due to material mass transfer different band in each micro-capsule.Wherein, when cell culture micro-capsule size needs When adjusting, central controller 17 receives the first Regulate signal of the transmission of touch-screen system 1, according to the first Regulate signal to push rod The transmission speed signal of system 14, the injection speed of injecting systems 15 is adjusted, the injection of injecting systems 15 is adjusted by push rod system 14 Speed control adjusts cell culture micro-capsule size.When cell culture micro-capsule size needs adjustment, central controller 17 receives to touch The second Regulate signal of the transmission of screen system 1 is touched, lifting signal is sent to jacking system 13 according to the second Regulate signal, passes through adjustment The one end of injecting systems 15 controls the electric-field intensity of high-voltage electrostatic field with the distance of negative plate 12, and adjustment institute is controlled by electric-field intensity State cell culture micro-capsule size.When cell culture micro-capsule size needs adjustment, by the output voltage for adjusting high voltage power supply 18 The electric-field intensity of high-voltage electrostatic field is controlled, the cell culture micro-capsule size is adjusted by electric-field intensity control.

Simultaneously as central controller 17, which receives touch-screen screen 1-2, sends control signal, it is to lifting according to control signal System 13 and the sending action signal of push rod system 14, by Automated condtrol, eliminate manual operation error, each micro-capsule size can be with Good uniformity is kept so as to ensure the accuracy of the homogeneity of cell culture and experiment.

By the Real Time Observation three-dimensional cell cultivation micro-capsule of culture dish observation window 5 feelings can occur for experimenter in experimentation Condition, by the Real Time Observation syringe 15-4 ejection situations of syringe observation window 7, experimenter can real-time regulation experiment as needed Parameter, to generate optimum cell dimensional culture micro-capsule.

After the completion of experiment, when opening switch gate 4, microswitch boss 4-2 and microswitch 13-9 departs from, microswitch 13-9 disconnects, i.e., circuit disconnects, and with Protection operating personnel, realizes enabling defencive function.Controller 17 controls push rod system 14 and jacking system 13 automatically reply initial position, it is (syringe 15-4, defeated that experimenter takes out culture dish 9 and medical disposable material Liquid pipe 15-5, stainless pin 15-6).

It should be noted last that above embodiment is only illustrating the technical solution of the utility model rather than limit System, although the utility model is described in detail with reference to example, it will be understood by those within the art that, can be right The technical solution of the utility model is modified or equivalent substitution, without departing from the spirit and model of technical solutions of the utility model Enclose, it all should cover among right of the present utility model.

Claims (10)

1. A kind of 1. automatic cytological dimensional culture microcapsule generating apparatus, it is characterised in that including：

   First housing (2)；

   Second housing (3), it is connected with first housing (2)；

   Touch-screen system (1), it is arranged on first housing (2)；

   Switch gate (4), it is flexibly connected with the opening of second housing (3)；

   Culture dish base (10), it is arranged on the switch gate (4), culture dish (9) is provided with the culture dish base (10)；

   Insulating base (11), it is arranged in second housing (3), negative plate (12) is enclosed with the insulating base (11)；

   Jacking system (13), it is arranged in second housing (3)；

   Push rod system (14), it is arranged in first housing (2)；

   Injecting systems (15), one end are connected with the jacking system (13), and the other end is connected with first housing (2), described Push rod system (14) promotes the injecting systems other end, when the switch gate (4) is closed, the second housing (3) closing, The culture dish base (10) is arranged on above the negative plate (12), and one end of the injecting systems (15) is placed in the culture Above ware (9)；

   High voltage power supply (18), it is arranged in second housing (3), positive pole and the jacking system of the high voltage power supply (18) Connection, the negative pole of the high voltage power supply (18) are connected with the negative plate；

   Central controller (17), it is arranged in second housing (3), the central controller (17) and the touch-screen system (1), jacking system (13) and push rod system (14) connection；

   Wherein, the central controller (17) receives the touch-screen system (1) and sends control signal, according to control signal to institute State jacking system (13) and push rod system (14) sending action signal.
2. 2. generating means according to claim 1, it is characterised in that：

   USB interface (19) is provided with first housing (2), the USB interface (19) connects with the central controller (17) Connect, set the injecting systems (15) place to be movably set with syringe cover plate (6), the syringe on first housing (2) Syringe observation window (7) is provided with cover plate (6).
3. 3. generating means according to claim 1, it is characterised in that：

   Heat emission hole (3-2) is offered on second housing (3), partition wall (3-1) is provided with second housing (3), it is described Second housing (3) is divided into the first accommodation space and the second accommodation space by partition wall, and the jacking system (13) is arranged on In first accommodation space, the high voltage power supply (18) and central controller (17) are arranged in second accommodation space.
4. 4. generating means according to claim 1, it is characterised in that：

   Culture dish observation window (5) and indent handle (4-1) are offered on the switch gate (4).
5. 5. generating means according to claim 1, it is characterised in that：

   The touch-screen system (1) includes：Touch-screen mounting bracket (1-1), rotating shaft installing plate (1-4), touch-screen (1-2) and touch Touch screen protecting crust (1-3)；

   Described touch-screen mounting bracket (1-1) one end is provided with spanner boss (1-1-1)；

   Described rotating shaft installing plate (1-4) one end is connected by Damping rotation shaft (1-5) and touch-screen mounting bracket (1-1) other end Connect, the other end is connected with first housing (2)；

   The touch-screen (1-2) is arranged in the touch-screen mounting bracket (1-1), the touch-screen and the central controller (17) connect, control signal is sent to the central controller (17)；

   The touch-screen protecting crust (1-3) is connected with the touch-screen mounting bracket (1-1).
6. 6. generating means according to claim 1, it is characterised in that：

   The jacking system (13) includes：Lift mounting bracket (13-2), lifting motor (13-1), syringe needle driving arm (13-4), slide card (13-3), insulation connecting plate (13-5), syringe needle card (13-7), woven hose clip (13-6), knocker female end (13-10) and microswitch (13-9)；

   Mounting bracket (13-2) is lifted, is arranged in second housing (3), notch is offered in the lifting mounting bracket, Scale is provided with the lifting mounting bracket (13-2)；

   Motor (13-1) is lifted, is arranged on the lifting mounting bracket (13-2), the lifting motor (13-1) It is connected with the central controller (17), receives the action signal of the central controller (17) transmission；

   Syringe needle driving arm (13-4), it is connected through the notch with the lifting motor (13-1)；

   Card (13-3) is slided, is connected with the syringe needle driving arm (13-4), the slip card (13-3) and the notch Position is consistent；

   Insulated connecting plate (13-5), and one end is connected with the syringe needle driving arm (13-4)；

   Syringe needle card (13-7), it is connected with described insulation connecting plate (13-5) other end, the syringe needle card (13-7) and the high pressure The positive pole connection of power supply (18), the syringe needle card (13-7) are connected with the injecting systems (15)；

   Woven hose clip (13-6), it is arranged on the insulation connecting plate (13-5)；

   Knocker female end (13-10), it is arranged on the lifting mounting bracket (13-2), knocker is provided with the switch gate (4) Sub- end, when the switch gate (4) is closed, the knocker female end (13-10) is flexibly connected with the sub- end of the knocker；

   Microswitch (13-9), it is arranged on the lifting mounting bracket (13-2), is provided with fine motion on the switch gate (4) and opens Boss (4-2) is closed, when the switch gate (4) is closed, the microswitch (13-9) connects with the microswitch boss (4-2) Touch, microswitch (13-9) closure.
7. 7. generating means according to claim 1, it is characterised in that：

   The push rod system (14) includes：Propel fixed support (14-2), propel motor (14-1), injector push-rod (14- 3), push rod cap (14-4) and proximity transducer (14-5)；

   The fixed support (14-2) of propelling is arranged in the first housing (2)；

   It is described propel motor (14-1) be arranged on it is described propel on fixed support (14-2), it is described to propel motor (14- 1) it is connected with the central controller (17), receives the action signal of the central controller (17) transmission；

   The injector push-rod (14-3) is connected with the motor (14-1) of propelling；

   The push rod cap (14-4) is arranged on the injector push-rod (14-3), and the push rod cap (14-4) promotes the injection One end of system (15)；

   The proximity transducer (14-5) is arranged on the push rod cap (14-4), the proximity transducer (14-5) with it is described in Controller (17) connection is entreated, position feed back signal is sent to the central controller (17).
8. 8. generating means according to claim 1, it is characterised in that：

   The injecting systems include：Syringe mounting blocks (15-1), syringe (15-2), woven hose (15-3) and draw point (15- 4)；

   The syringe mounting blocks (15-1) are connected with first housing (2)；

   The syringe (15-2) is arranged on the syringe mounting blocks (15-1)；

   Described woven hose (15-3) one end connects with the syringe (15-2)；

   The draw point (15-4) connects with the woven hose (15-3) other end, and the draw point (15-4) is arranged on the lifting system Unite on (13).
9. 9. generating means according to claim 1, it is characterised in that the automatic cytological dimensional culture micro-capsule fills Putting also includes：

   Disinfection system, it is arranged in second housing (3).
10. 10. generating means according to claim 9, it is characterised in that the disinfection system includes：

    Uviol lamp (16), it is arranged in second housing (3).