CN206916126U - A kind of pine wood nematode nucleic acid-extracting apparatus - Google Patents

A kind of pine wood nematode nucleic acid-extracting apparatus Download PDF

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Publication number
CN206916126U
CN206916126U CN201720850523.4U CN201720850523U CN206916126U CN 206916126 U CN206916126 U CN 206916126U CN 201720850523 U CN201720850523 U CN 201720850523U CN 206916126 U CN206916126 U CN 206916126U
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CN
China
Prior art keywords
cover body
drill
surge drum
nucleic acid
annular groove
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201720850523.4U
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Chinese (zh)
Inventor
邱秀文
周桂香
何龙喜
谭世才
王慧娟
胡皓
王薇
赵爱德
王晶
汪湘君
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Jiujiang University
Original Assignee
Jiujiang University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiujiang University filed Critical Jiujiang University
Priority to CN201720850523.4U priority Critical patent/CN206916126U/en
Application granted granted Critical
Publication of CN206916126U publication Critical patent/CN206916126U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model provides a kind of pine wood nematode nucleic acid-extracting apparatus, including electric drill, and electric drill includes drill body and drill bit, and drill bit is arranged on the front end of drill body, and drill body head is additionally provided with cover body, and cover body is provided with surge drum;Cover body is tubular, the rear end of cover body and the front end of drill body are connected by spring, the front end of spring is connected with cover body, the rear end of spring is connected by connector with drill body, connector includes bottom plate and is arranged on the edge of bottom plate side, connector is socketed by edge and drill body, bottom plate is provided with centre bore, drill bit is fed through centre bore and is arranged in cover body, cover body is provided with annular groove, and annular groove is provided with opening, the open at one end of surge drum, the other end is closed, and the opening of the open one end of surge drum and annular groove connects.Pine wood nematode nucleic acid-extracting apparatus provided by the utility model, wood chip can have an effect after entering surge drum with lysate, be cleaved pine wood nematode, simple in construction, easy to operate.

Description

A kind of pine wood nematode nucleic acid-extracting apparatus
Technical field
It the utility model is related to a kind of nucleic acid-extracting apparatus, more particularly to a kind of pine wood nematode nucleic acid-extracting apparatus.
Background technology
The pine tree wilt disease as caused by endangering pine wood nematode is destructive disease especially big in forestry, be it is internationally recognized most Important plant quarantine harmful organism.With the progress of Protocols in Molecular Biology, the detection technique of pine wood nematode is also increasingly It is more.Application No. 201220581808.X, notification number are CN 203007255U, and the day for announcing is that 2013.6.19 patent discloses Pine wood nematode nucleic acid rapid extraction device in a kind of timber, including the timber collection device and nucleic acid speed that are used in conjunction with each other carry dress Put;Timber acquisition device includes collector, drill bit and electric drill;Nucleic acid speed, which carries device, includes syringe, filter, adsorption column and suction Head, adsorbed film is set in adsorption column;When collecting wood chip, drill bit is connected with electric drill, collector be located at drill bit and timber to be measured it Between;Syringe is set below collector;Extract adjust when, syringe first be connected with suction nozzle absorption lysate, then with filtering Device, adsorption column and suction nozzle are sequentially connected extraction nucleic acid.Although the device can directly extract the nucleic acid in sample, complicated, It is cumbersome, it is inconvenient for use.
Utility model content
The purpose of this utility model is to provide a kind of pine wood nematode nucleic acid-extracting apparatus solve the existing knot of prior art Structure is complicated, it is cumbersome the problem of.
The technical solution of the utility model is as follows:A kind of pine wood nematode nucleic acid-extracting apparatus, including electric drill, electric drill include boring Body and drill bit, drill bit are arranged on the front end of drill body, and drill body head is additionally provided with cover body, and cover body is provided with surge drum;Cover body is tubular, Spring is provided between the rear end of cover body and the front end of drill body, the front end of spring is connected with cover body, and the rear end of spring passes through connector It is connected with drill body, connector includes bottom plate and is arranged on the edge of bottom plate side, and connector is socketed by edge and drill body, bottom plate Centre bore is provided with, drill bit is arranged in cover body through the centre bore, and the inwall of cover body is annular groove, and annular groove is provided with Opening, surge drum are arranged on the opening on annular groove, the open at one end of surge drum, and the other end is closed, surge drum opening The opening of one end and annular groove connects.
Preferably, it is to be detachably connected between above-mentioned surge drum and cover body.
Preferably, above-mentioned surge drum is provided with feed tube.
The utility model has the advantages that:Pine wood nematode nucleic acid-extracting apparatus provided by the utility model, cover body It is connected with drill body by spring, the step of eliminating handling, and does not influence the traveling of drill bit;Cover body inwall is annular groove and set There is surge drum, avoid the waste of wood chip, wood chip can have an effect after entering surge drum with lysate, be split pine wood nematode Solution, so as to discharge nucleic acid.Pine wood nematode nucleic acid-extracting apparatus provided by the utility model, simple in construction, easy to operate, work effect Rate is high.
Brief description of the drawings
Fig. 1 is the pine wood nematode nucleic acid-extracting apparatus structural representation that the utility model embodiment provides.
In figure, 1. drill bodies, 2. drill bits, 3. cover bodies, 4. springs, 5. connectors, 6. annular grooves, 7. surge drums, 8. feed liquors Pipe.
Embodiment
Specific embodiment of the present utility model is described in further detail below in conjunction with the accompanying drawings.
Embodiment
A kind of pine wood nematode nucleic acid-extracting apparatus, referring to Fig. 1, including electric drill, electric drill includes drill body 1 and drill bit 2, drill bit 2 The front end of drill body 1 is arranged on, the front end of drill body 1 is additionally provided with cover body 3, and cover body 3 is provided with surge drum 7;Cover body 3 is tubular, cover body 3 Spring 4 is provided between rear end and the front end of drill body 1, the front end of spring 4 is connected with cover body 3, and the rear end of spring 4 passes through connector 5 Be connected with drill body 1, connector 5 includes bottom plate and is arranged on the edge of bottom plate side, be block shape, connector 5 by edge and Drill body 1 is socketed, and the bottom plate of connector 5 is provided with centre bore, and drill bit 2 is arranged in cover body 3 through centre bore, and the inwall of cover body 3 is Annular groove 6, annular groove 6 is circumferentially disposed along cover body 3, and annular groove 6 is provided with opening, and opening is provided with surge drum 7, receives Collect the open at one end of cylinder 7, other end closing, the open one end of surge drum 7 is connected with the opening of annular groove 6.
In use, adding lysate in surge drum 7, surge drum 7 is connected with cover body 3, electric drill is opened and drills through wood chip, wood Bits enter in annular groove 6, and the lysate entered by annular groove 6 in surge drum 7, with surge drum 7 is had an effect, pine line Worm is cleaved, and discharges nucleic acid.Then, lysate is filtered, is filtered impurity therein, then by adsorbed film, make it In nucleic acid be adsorbed, finally by adsorbed film Nucleic Acid Elution collect.
It is to be detachably connected in order to easy to loading and unloading, preferably between surge drum 7 and cover body 3.In the present embodiment, surge drum 7 with The opening of annular groove 6 is threaded connection, can also use mounting or clamping.
For the ease of the connection of surge drum 7 and cover body 3, preferably surge drum 7 is provided with feed tube 8.In use, can be first by sky Surge drum 7 be connected on cover body 3, then again pass through feed tube 8 add lysate.
Pine wood nematode nucleic acid-extracting apparatus provided by the utility model, cover body 3 are connected with drill body 1, eliminate the step of handling Suddenly, it is easy to operate;Spring 4 is provided between cover body 3 and drill body 1, when drill bit 2 is advanced, the compression spring 4 of cover body 3 retreats, and does not influence The traveling of drill bit 2;The inwall of cover body 3 is annular groove 6, and the wood chip that drill bit 2 gathers is directly entered in annular groove 6, avoids wood The waste of bits, improves collecting efficiency;Annular groove 6 is provided with surge drum 7, and lysate is added in surge drum 7, and wood chip, which enters, to be received It can be had an effect after collection cylinder 7 with lysate, be cleaved pine wood nematode, so as to discharge nucleic acid.Pine provided by the utility model Material nematode nucleic acid extraction element, simple in construction, compact, simple to operate, easy to use, operating efficiency is high.

Claims (3)

  1. A kind of 1. pine wood nematode nucleic acid-extracting apparatus, it is characterised in that:Including electric drill, the electric drill includes drill body (1) and drill bit (2), the drill bit (2) is arranged on the front end of drill body (1), and drill body (1) front end is additionally provided with cover body (3), on the cover body (3) Provided with surge drum (7);Cover body (3) is tubular, is connected between the rear end and the front end of drill body (1) of cover body (3) by spring (4), The front end of the spring (4) is connected with cover body (3), and the rear end of spring (4) is connected by connector (5) with drill body (1), the company Fitting (5) includes bottom plate and is arranged on the edge of bottom plate side, and connector (5) is socketed by the edge and cover body (3), connection The bottom plate of part (5) is provided with centre bore, and drill bit (2) is arranged in cover body (3) through the centre bore, and the inwall of cover body (3) is Annular groove (6), the annular groove (6) are provided with opening, and the surge drum (7) is arranged on the opening on annular groove (6) Place, the open at one end of the surge drum (7), other end closing, the open one end of surge drum (7) and the opening of annular groove (6) Place's connection.
  2. 2. pine wood nematode nucleic acid-extracting apparatus as claimed in claim 1, it is characterised in that:The surge drum (7) and cover body (3) Between to be detachably connected.
  3. 3. pine wood nematode nucleic acid-extracting apparatus as claimed in claim 1, it is characterised in that:The surge drum (7) be provided with into Liquid pipe (8).
CN201720850523.4U 2017-07-13 2017-07-13 A kind of pine wood nematode nucleic acid-extracting apparatus Expired - Fee Related CN206916126U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720850523.4U CN206916126U (en) 2017-07-13 2017-07-13 A kind of pine wood nematode nucleic acid-extracting apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720850523.4U CN206916126U (en) 2017-07-13 2017-07-13 A kind of pine wood nematode nucleic acid-extracting apparatus

Publications (1)

Publication Number Publication Date
CN206916126U true CN206916126U (en) 2018-01-23

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201720850523.4U Expired - Fee Related CN206916126U (en) 2017-07-13 2017-07-13 A kind of pine wood nematode nucleic acid-extracting apparatus

Country Status (1)

Country Link
CN (1) CN206916126U (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111296383A (en) * 2020-02-24 2020-06-19 菏泽学院 Hand-held pine wood nematode insect killer
CN118667648A (en) * 2024-08-26 2024-09-20 四川杰莱美科技有限公司 Artificial intelligence-based pine wood nematode nucleic acid detection system and method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111296383A (en) * 2020-02-24 2020-06-19 菏泽学院 Hand-held pine wood nematode insect killer
CN118667648A (en) * 2024-08-26 2024-09-20 四川杰莱美科技有限公司 Artificial intelligence-based pine wood nematode nucleic acid detection system and method

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CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20180123

Termination date: 20180713