CN206872823U - A kind of anaerobe High pressure culture device of convenient for taking liquid - Google Patents

Abstract

The utility model discloses a kind of anaerobe High pressure culture device of convenient for taking liquid, the culture apparatus includes culture tube body, the culture tube body includes high pipe end, horizontal ends and low pipe end, the high pipe end is set with low pipe end side-by-side parallel, the horizontal ends connect high pipe end and low pipe end, the internal diameter of the pipeline of the low pipe end is more than high pipe end and the internal diameter of the pipeline of horizontal ends, and the upper portion side wall of the low pipe end sets liquid outlet, valve is installed on the liquid outlet.The characteristics of anaerobe High pressure culture device of the present utility model possesses takes liquid convenient under hyperbaric environment, will not occur the phenomenon of liquid taking port blocking, and slag thing is less in the liquid of taking-up, the centrifugation number of liquid can be reduced, improve the efficiency of liquid detecting.

Description

A kind of anaerobe High pressure culture device of convenient for taking liquid

Technical field

The utility model belongs to the High pressure culture engineering device technique field of anaerobe, and in particular to a kind of convenient for taking liquid Anaerobe High pressure culture device.

Background technology

Anaerobe is a kind of suitable microorganism grown under anaerobic, mainly includes anaerobic type bacterium, also wraps Include a small amount of actinomyces and mycoplasma, the anaerobic fungi of only a few.Anaerobe is extensive in distributed in nature, and species is various, They are in close relations with the mankind, therefore the research to anaerobe also increasingly draws attention.

The existing training method to anaerobe mainly has Heng Shi anaerobism rolling tube technique, anaerobic jar technology, anaerobism gloves Box technology, culture medium deoxygenation technology, airbag anaerobic system and anaerobic petri diss technology etc..It is conventional except being carried out to anaerobe Anaerobic culturel research it is outer, at this stage due to finding that part anaerobe possesses from the special physiological environment such as oil reservoir, ocean High voltage bearing characteristic, and this quasi-microorganism has and is different from special genotype, metabolic mechanism and metabolite under normal pressure, therefore More important is seemed to culture of the anaerobe under hyperbaric environment and the research of metabolic characteristics.

During High pressure culture is carried out to anaerobe, generally require to examine the liquid in High pressure culture pipe Survey, to understand the vital movement of anaerobe, but the existing culture that anaerobe is carried out using High pressure culture pipe, mostly It is that liquid taking port is set in High pressure culture bottom of the tube, if the High pressure culture pipe in the U of patent CN 204939469 is from culture tube bottom Portion takes liquid.But said apparatus on the one hand due to the pressure in High pressure culture pipe it is very big, on the other hand, due to anaerobe train High pressure culture bottom of the tube remains more slag thing during supporting, and when carrying out taking liquid from High pressure culture bottom of the tube, easily blocks Liquid taking port, and contain more slag thing in the liquid taken out, it is past when being detected by ultraviolet spectrometer and liquid chromatograph Toward needing to be detected by multiple centrifugation operation, the detection efficiency and testing result of liquid have been had a strong impact on Accuracy.Therefore, there is an urgent need to be improved to High pressure culture pipe device, liquid not side is taken to solve existing High pressure culture pipe Just, the problem of slag thing easily blocks liquid taking port, and slag thing is more in the liquid of taking-up.

Utility model content

The purpose of this utility model is exactly in order to solve the above-mentioned technical problem, and to provide a kind of micro- life of the anaerobism of convenient for taking liquid Thing High pressure culture device, possess and the characteristics of liquid is convenient, and slag thing is less in the liquid of taking-up is taken under hyperbaric environment, can avoid taking Liquid mouth blocks, and can reduce the centrifugation number of liquid, improves the efficiency of liquid detecting.

To achieve these goals, the technical solution adopted in the utility model is：A kind of anaerobe of convenient for taking liquid High pressure culture device, including culture tube body, the culture tube body include high pipe end, horizontal ends and low pipe end, the senior executive End is set with low pipe end side-by-side parallel, and the horizontal ends connect high pipe end and low pipe end, the internal diameter of the pipeline of the low pipe end are more than The internal diameter of the pipeline of high pipe end and horizontal ends, the upper portion side wall of the low pipe end set liquid outlet, valve are installed on the liquid outlet.

Usually used High pressure culture pipe is arranged to high pipe end, horizontal ends and low pipe end three parts by the utility model, high Pipe end is set with low pipe end using side-by-side parallel, and the liquid outlet by being arranged on low pipe end top is carried out taking liquid, will not only made Blocked into liquid taking port, and the content of slag thing in the liquid of taking-up can be greatly reduced, due to the Action of Gravity Field of slag thing, its big portion Divide the horizontal ends for being deposited on culture tube body, because the internal diameter of the pipeline of low pipe end is larger, slag thing is not easy to when carrying out taking liquid Enter from horizontal ends at the top of low pipe end and ooze out, so as to reduce the separation of subsequent liquid slagging-off number, improve liquid detecting Efficiency.

Further, the high pipe end, horizontal ends and low pipe end integrally form J-shaped, and horizontal ends are positioned at J-shaped minimum Point, high pipe end and low pipe end are respectively perpendicular to the both ends of horizontal ends, can largely prevent the slag things of horizontal ends from low pipe end Outflow.

Further, the height of the low pipe end is more than the 1/3 of high pipe end whole height, and the setting of the height can be larger Degree prevents the slag thing of horizontal ends from being flowed out from the liquid outlet on low pipe end top.

Further, filter screen is set in the horizontal ends, and filter screen can be good at preventing the slag thing of horizontal ends from entering low pipe End.

Further, the horizontal ends bottom sets slag-drip opening, when culture is multiple, slag thing in horizontal ends bottom deposit, Convenient discharge slag thing.

Further, the culture apparatus also includes access cover, and internal thread, the high pipe end are set inside the access cover Top sets the external screw thread to match with internal thread, the centrally disposed through hole of access cover, be installed with the through hole into Mouth is managed, and triple valve is installed on the inlet tube.Access cover can be connected and sealed by internal thread with high pipe end, and access cover is convenient Fed intake into culture tube body, the convenient connection of screw-type connection mode and dismounting, inlet tube can be realized to be filled into culture tube body Nitrogen is pressurized.

Further, first interface connection pressure gauge of the triple valve, second interface connection inlet tube, the 3rd Interface connects breather pipe, and two-way valve is installed on the breather pipe.One interface of two-way valve can connect nitrogen charging device, another Interface can connect collector.Inlet tube and high pipe end can be attached, nitrogen is then filled with into breather pipe and realizes training The high pressure anaerobic environment in tube body is supported, pressure gauge can detect the pressure change in inlet tube, and two-way valve is conveniently filled Gas and take gas.

The beneficial effects of the utility model are：The utility model by culture tube body reasonably improved, if It is set to including high pipe end, horizontal ends and low pipe end three parts, and according to structure design of the present utility model, enables to caused Slag thing is largely deposited on horizontal end position, convenient to carry out taking liquid from low pipe end, prevents slag thing from blocking liquid taking port, and the liquid taken out Slag thing content is few in body, and centrifugation slagging-off number is less, can greatly improve the efficiency of liquid detecting.

Brief description of the drawings

Fig. 1 is overall structure diagram of the present utility model；

Fig. 2 is access cover structural representation of the present utility model；

Fig. 3 is the structural representation that access cover is connected with high pipe end；

Wherein, 1 is high pipe end, and 2 be horizontal ends, and 3 be low pipe end, and 4 be liquid outlet, and 5 be valve, and 6 be filter screen, and 7 be deslagging Mouthful, 8 be access cover, and 9 be through hole, and 10 be inlet tube, and 11 be triple valve, and 12 be pressure gauge, and 13 be breather pipe, and 14 be two-way valve, 15 be internal thread, and 16 be external screw thread.

Embodiment

In order that the purpose of this utility model, technical scheme and advantage are more clearly understood, it is with reference to embodiments and attached The utility model is specifically described figure, it is necessary to, it is noted that following examples are used only for carrying out the utility model Explanation and illustration, it is not used to limit the utility model.Made according to the above some non-of those skilled in the art The modifications and adaptations of matter, still fall within the scope of protection of the utility model.

Embodiment

As Figure 1-3, it is a kind of anaerobe High pressure culture device of convenient for taking liquid provided by the utility model, should Microbial cultivation device includes culture tube body, and culture tube body is made up of high pipe end 1, horizontal ends 2 and low pipe end 3, wherein high Pipe end 1 is set with the low side-by-side parallel of pipe end 3, and horizontal ends 2 connect high pipe end 1 and low pipe end 3, high pipe end 1, horizontal ends 2 and low pipe End 3 is overall in J-shaped.The internal diameter of the pipeline of low pipe end 3 is more than high pipe end 1 and the internal diameter of the pipeline of horizontal ends 2, the height of low pipe end 3 More than the 1/3 of the high whole height of pipe end 1, filter screen 6 is set in the pipeline of horizontal ends 2, the bottom of horizontal ends 2 sets slag-drip opening 7, The upper portion side wall of low pipe end 3 is provided with liquid outlet 4, valve 5 is installed on liquid outlet 4.

Above-mentioned anaerobe High pressure culture device also includes access cover 8, and the inside of access cover 8 sets internal thread 15, senior executive The top of end 1 sets the external screw thread 16 to match with internal thread 15, the centrally disposed through hole 9 of access cover 8, is installed with through hole 9 Inlet tube 10, triple valve 11, first interface connection pressure gauge 12 of triple valve 11 are installed on inlet tube 10, and second interface connects Inlet tube 10 is connect, the 3rd interface connects breather pipe 13, two-way valve 14 is installed on breather pipe 13.

The liquid outlet 4 that the utility model is set on the low top of pipe end 3, it is convenient to carry out taking liquid under high pressure, in J-shaped training Tube body is supported, enables to most of slag thing to be deposited on the horizontal ends 2 of culture tube body, without blocking liquid outlet 4, by low pipe The height at end 3 sets 1/3 more than the high height of pipe end 1, and the slag thing in horizontal ends 2 can be avoided to be flowed out from liquid outlet 4, subtracted significantly The content of slag thing in the liquid taken out is lacked.

Claims (7)

1. a kind of anaerobe High pressure culture device of convenient for taking liquid, including culture tube body, it is characterised in that the culture Tube body includes high pipe end (1), horizontal ends (2) and low pipe end (3), and the high pipe end (1) sets with low pipe end (3) side-by-side parallel Put, the horizontal ends (2) connect high pipe end (1) and low pipe end (3), the internal diameter of the pipeline of the low pipe end (3) are more than high pipe end (1) With the internal diameter of the pipeline of horizontal ends (2), the upper portion side wall of the low pipe end (3) sets liquid outlet (4), pacified on the liquid outlet (4) Fill valve (5).

2. the anaerobe High pressure culture device of convenient for taking liquid according to claim 1, it is characterised in that the senior executive Holding (1), horizontal ends (2) and low pipe end (3), integrally composition is J-shaped.

3. the anaerobe High pressure culture device of convenient for taking liquid according to claim 1 or 2, it is characterised in that described The height of low pipe end (3) is more than the 1/3 of high pipe end (1) whole height.

4. the anaerobe High pressure culture device of convenient for taking liquid according to claim 1 or 2, it is characterised in that described Filter screen (6) is set in horizontal ends (2).

5. the anaerobe High pressure culture device of convenient for taking liquid according to claim 1 or 2, it is characterised in that described Horizontal ends (2) bottom sets slag-drip opening (7).

6. the anaerobe High pressure culture device of convenient for taking liquid according to claim 1 or 2, it is characterised in that also wrap Access cover (8) is included, internal thread (15) is set inside the access cover (8), set and internal thread (15) at the top of the high pipe end (1) The external screw thread (16) to match, the centrally disposed through hole of the access cover (8) (9), the through hole (9) is interior to be installed with inlet tube (10) triple valve (11), is installed on the inlet tube (10).

7. the anaerobe High pressure culture device of convenient for taking liquid according to claim 6, it is characterised in that the threeway First interface connection pressure gauge (12) of valve (11), second interface connection inlet tube (10), the 3rd interface connection ventilation Manage (13), two-way valve (14) is installed on the breather pipe (13).