CN206851797U - A kind of device for collecting cryptocaryon irritans larva - Google Patents
A kind of device for collecting cryptocaryon irritans larva Download PDFInfo
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- CN206851797U CN206851797U CN201720762527.7U CN201720762527U CN206851797U CN 206851797 U CN206851797 U CN 206851797U CN 201720762527 U CN201720762527 U CN 201720762527U CN 206851797 U CN206851797 U CN 206851797U
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- filter
- collecting
- filtering head
- core filtering
- larva
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Abstract
A kind of device for collecting cryptocaryon irritans larva, is related to laboratory sample collection device.A kind of a kind of device of collection cryptocaryon irritans larva simple in construction, easy to operate, can collecting a large amount of vibrant larvas is provided.Provided with filtration fraction, geometrical clamp, liquid-collecting bottle and vavuum pump；The filtration fraction is provided with filter cup, filter screen, filter paper and core filtering head, and filter screen and filter paper are fixed between filter cup and core filtering head by the geometrical clamp, the top of the core filtering head and filter cup and the bottom connection of liquid-collecting bottle；The liquid-collecting bottle is connected by ground bottleneck with core filtering head；The core filtering head is connected by sebific duct with vavuum pump.
Description
Technical field
Laboratory sample collection device is the utility model is related to, more particularly, to a kind of dress for collecting cryptocaryon irritans larva
Put.
Background technology
Cryptonucleus insect (Cryptocaryon irritans) is stimulated, belongs to cryptonucleus insect section (Cryptocaryonidae) hidden core
Eimeria (Cryptocaryon) member, is widely distributed in tropical and subtropical region, can cause the cause of a variety of marine fish
Dead property disease is " ichthyophthirius ", is the class epidemic disease of China two.The history of life of cryptonucleus insect is stimulated to be divided into 4 stages:Before trophozoite, packing
Body, packing and larva.Trophozoite is unique parasitic stages, leaves variance in form precursor after host, packing precursor is very in short-term
Interior development is packing and is attached to water-bed and divides under suitable conditions.This 3 periods stimulate cryptonucleus insect individual larger, long
More than 400~500 μm, caused larva can quickly move about degree in water after packing division, have very strong infection ability, but
This ability is most strong in 2h after excystment, and 8h postoperative infection abilities significantly weaken.In recent years, cryptocaryoniosis is stimulated frequently to break out,
Getting worse is endangered, stimulates the research of cryptonucleus insect also more and more to be paid attention to.Cryptonucleus insect is stimulated to pass on and cultured in vitro
Research is a lot, and a large amount of collections of packing and trophozoite also have significant component of work, and the collection of larva also rests on centrifugation
Obtain in this method.In order to study to stimulating the immune protection of cryptonucleus insect, it is necessary to substantial amounts of stimulation cryptonucleus insect be collected, due to thorn
Swashing cryptonucleus insect trophozoite, packing precursor and packing are relatively large in diameter (more than 300~500 μm), and naked eyes are visible, are relatively easy to collect, and
Larva is small and easily rupturable, a large amount of to collect more difficulty.Existing research is all to use centrifugal process at present, and this method efficiency is low, right
Larva injury is big, it is therefore necessary to designs a kind of safe and efficient device for collecting larva.
The content of the invention
The problem of the purpose of this utility model is to exist for prior art provides solution, there is provided a kind of structure letter
A kind of device of collection cryptocaryon irritans larva single, easy to operate, that a large amount of vibrant larvas can be collected.
The utility model is provided with filtration fraction, geometrical clamp, liquid-collecting bottle and vavuum pump；The filtration fraction be provided with filter cup,
Filter screen, filter paper and core filtering head, filter screen and filter paper are fixed between filter cup and core filtering head by the geometrical clamp, described
The bottom connection on the top and liquid-collecting bottle of core filtering head and filter cup；The liquid-collecting bottle passes through ground bottleneck and core filtering head
Connection；The core filtering head is connected by sebific duct with vavuum pump.
The filter cup and liquid-collecting bottle are Large Copacity glassware.
The filter screen can use 400 mesh filter screens, and the filter paper can use 0.22 μm of filter paper.
The filter screen is supported by core filtering head with filter paper and leaves space between the two, for separating in larva and water
Bacterium and fungi.
With it is existing the method for cryptocaryon irritans larva is collected by centrifugation compared with, the utility model is big according to cryptocaryon irritans larva
It is small notable difference to be present with other stage of development sizes to separate larva.Space between filter screen and filter paper freely to move about in water
Larva can be separated with most of bacterium, fungi in water body, be adapted to for stimulate cryptonucleus insect test subsequent request；With difference
Aperture filter screen obtains the lossless larvas of a large amount of health, hence it is evident that the treatment research better than centrifugal process to cryptocaryon irritans larva；Together
When can also be applied to breeding process in remove water body parasite.
Brief description of the drawings
Fig. 1 is the structural representation of the utility model embodiment.
Embodiment
Below in conjunction with drawings and Examples, the utility model is described in further detail.
As shown in figure 1, the utility model embodiment is provided with filter cup 1, filtering head 2, liquid-collecting bottle 3, filter screen 4, filter paper 5, sand
Core filtering head 6, sebific duct 7, vavuum pump 8 and geometrical clamp 9；Filter screen 4 and filter paper 5 are fixed on filter cup 1 and core by the geometrical clamp 9
Between filtering head 6, the filtering head 2 is arranged on the top of liquid-collecting bottle 3, and the top of filtering head 2 is connected with core filtering head 6, described
Liquid-collecting bottle 3 is connected by ground bottleneck with filtering head 2；The filtering head 2 is connected by sebific duct 7 with vavuum pump 8.
The filter cup 1 and liquid-collecting bottle are Large Copacity glassware.
The filter screen 4 uses 400 mesh filter screens, and the filter paper 5 uses 0.22 μm of filter paper.
The filter screen 4 is supported by core filtering head 6 with filter paper 5 and leaves space between the two, for separating larva and water
In bacterium and fungi.
Specific embodiment given below.
Hatching stimulates cryptonucleus insect packing under the conditions of being 20 ‰~25 ‰ in 23~27 DEG C of temperature, salinity, and collection is incubated as far as possible
Change the larva in 2h.During using collection device, first sterilizing seawater is added in filter cup 1, vavuum pump 8 is opened and is taken out by sebific duct 7
Walk the gas in liquid-collecting bottle 3 so that the seawater in filter cup 1 enters in liquid-collecting bottle 3.By the seawater containing cryptocaryon irritans larva
It is poured slowly into filter cup 1, keeps having seawater always in filter cup 1, cryptocaryon irritans larva can freely be swum in filter cup 1
Move without being flowed away with seawater so as to reach enrichment purpose.It can be abandoned after liquid is filled in liquid-collecting bottle 3 after vavuum pump 8 is turned off
Go.When needing to collect cryptocaryon irritans larva, stop adding the seawater containing cryptocaryon irritans larva, sea in cup 1 to be filtered
Vavuum pump 8 is closed immediately during water residual quantity about 5ml, removes geometrical clamp 9 after seawater is finally fully entered in liquid-collecting bottle 3, it is small
The heart uses the slow flushing filtering net of a small amount of sterilizing seawater to can obtain a large amount of healthy cryptocaryon irritans larvas after taking out filter screen 4.
Claims (4)
1. a kind of device for collecting cryptocaryon irritans larva, it is characterised in that provided with filtration fraction, geometrical clamp, liquid-collecting bottle and vacuum
Pump；The filtration fraction is provided with filter cup, filter screen, filter paper and core filtering head, and filter screen and filter paper are fixed on by the geometrical clamp
Between filter cup and core filtering head, the top of the core filtering head and filter cup and the bottom connection of liquid-collecting bottle；The collection
Liquid bottle is connected by ground bottleneck with core filtering head；The core filtering head is connected by sebific duct with vavuum pump.
2. a kind of device for collecting cryptocaryon irritans larva as claimed in claim 1, it is characterised in that the filter screen uses 400 mesh
Filter screen.
3. a kind of device for collecting cryptocaryon irritans larva as claimed in claim 1, it is characterised in that the filter paper uses 0.22 μ
M filter paper.
A kind of 4. device for collecting cryptocaryon irritans larva as claimed in claim 1, it is characterised in that the filter screen and filter paper by
Core filtering head supports and leaves space between the two.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720762527.7U CN206851797U (en) | 2017-06-28 | 2017-06-28 | A kind of device for collecting cryptocaryon irritans larva |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720762527.7U CN206851797U (en) | 2017-06-28 | 2017-06-28 | A kind of device for collecting cryptocaryon irritans larva |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206851797U true CN206851797U (en) | 2018-01-09 |
Family
ID=60828773
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720762527.7U Expired - Fee Related CN206851797U (en) | 2017-06-28 | 2017-06-28 | A kind of device for collecting cryptocaryon irritans larva |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206851797U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108619769A (en) * | 2018-05-07 | 2018-10-09 | 华中科技大学 | A kind of suction filtration system and solid-liquid separating method being suitable for separation of solid and liquid |
| CN110754436A (en) * | 2019-10-31 | 2020-02-07 | 青岛农业大学 | Method for collecting larvae of cryptocaryon irritans |
| CN112795484A (en) * | 2021-02-08 | 2021-05-14 | 自然资源部第四海洋研究所(中国—东盟国家海洋科技联合研发中心) | Method for extracting and separating exosome of cryptocaryon irritans of marine parasitic ciliates and application of exosome |
-
2017
- 2017-06-28 CN CN201720762527.7U patent/CN206851797U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108619769A (en) * | 2018-05-07 | 2018-10-09 | 华中科技大学 | A kind of suction filtration system and solid-liquid separating method being suitable for separation of solid and liquid |
| CN110754436A (en) * | 2019-10-31 | 2020-02-07 | 青岛农业大学 | Method for collecting larvae of cryptocaryon irritans |
| CN112795484A (en) * | 2021-02-08 | 2021-05-14 | 自然资源部第四海洋研究所(中国—东盟国家海洋科技联合研发中心) | Method for extracting and separating exosome of cryptocaryon irritans of marine parasitic ciliates and application of exosome |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180109 Termination date: 20180628 |