CN206756847U - Duck source NDV, duck plague virus, duck circovirus colloidal-gold detecting-card - Google Patents
Duck source NDV, duck plague virus, duck circovirus colloidal-gold detecting-card Download PDFInfo
- Publication number
- CN206756847U CN206756847U CN201720248195.0U CN201720248195U CN206756847U CN 206756847 U CN206756847 U CN 206756847U CN 201720248195 U CN201720248195 U CN 201720248195U CN 206756847 U CN206756847 U CN 206756847U
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- China
- Prior art keywords
- duck
- gold
- card
- ndv
- colloidal
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- Expired - Fee Related
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Abstract
It the utility model is related to a kind of duck source NDV, duck plague virus, duck circovirus colloidal-gold detecting-card, the colloidal-gold detecting-card includes loading pad, standard gold pad, test strips, absorbent filter and PVC bottom plates, the loading pad, standard gold pad are put in detection card loading endlap, the other end is stacked with absorbent filter, and the bottom surface of the test strips is PVC bottom plates；The test strips are coated with the first detection line of duck source NDV, are coated with the second detection line of duck plague virus, are coated with the 3rd detection line and nature controlling line of duck circovirus cap albumen successively since loading end；The anti-duck IgG antibody of mouse of colloid gold label is coated with the standard gold pad.Duck source of the present utility model NDV, duck plague virus, the colloidal-gold detecting-card of duck circovirus three detection during, required sample size is few, kit it is easy to operate, detection sample is easily obtained, easy to detect quick, immune response specificity is high, and result judgement is convenient.
Description
Technical field
It the utility model is related to detection card technique field, and in particular to a kind of duck source NDV, duck plague virus, duck circle
The colloidal-gold detecting-card of circovirus virus three.
Background technology
At present, the clinical common three kinds of virus mixed infections detection of duck source NDV, duck plague virus, duck circovirus is more
Individually to be detected, it is necessary to be detected respectively, its is time-consuming, arduously.Existing detection method also has PCR kit to be examined
Survey, but this detection method, the time length required for detecting, the cost of detection are high.The existing detection method of these three viruses
It is the features such as easily polluting, be cumbersome, poor specificity be present more.
As can be seen here, there is cost in traditional detection duck source NDV, duck plague virus, the instrument of duck circovirus
Between high, working costs, poor specificity, it is cumbersome the defects of, it would be highly desirable to further improve.
Utility model content
The purpose of this utility model is to provide a kind of duck source NDV, duck plague virus, the glue of duck circovirus three
Body gold detection card, to solve the problems, such as that prior art is present.
To realize above-mentioned technical problem, the utility model provides a kind of duck source NDV, duck plague virus, duck annulus disease
Malicious three colloidal-gold detecting-cards, the colloidal-gold detecting-card include test strips, loading pad, standard gold pad and blotting paper, test strips
The top at loading end be stacked the loading pad, standard gold pad, the other end of test strips is stacked with absorbent filter, the test strips
Bottom surface is PVC bottom plates；
The test strips are coated with the first detection line of duck source NDV, coating duck plague disease successively since loading end
Second detection line of poison, the 3rd detection line and nature controlling line for being coated with duck circovirus cap albumen；
The anti-duck IgG antibody of mouse of colloid gold label is coated with the standard gold pad.
In one embodiment of the present utility model, the nature controlling line is coated with sheep anti-mouse igg.
In one embodiment of the present utility model, the collaurum is 38-42nm spheric granules.
In one embodiment of the present utility model, the test strips, loading pad and standard gold pad are nitrocellulose membrane.
In one embodiment of the present utility model, the colloidal-gold detecting-card also includes buckle box, and the buckle box includes bottom
Seat and lid, the test strips, loading pad, standard gold pad and blotting paper are fixed by buckle box.
In one embodiment of the present utility model, one end that the lid corresponds to the loading pad offers circular loading
Hole, correspond to and offer rectangular form at the detection line and nature controlling line of test strips.
In one embodiment of the present utility model, the radius in the loading hole is 0.15-0.35cm.
In one embodiment of the present utility model, a length of 11-12cm of the buckle box, a width of 2-4cm, thickness 0.2-
0.4cm。
The utility model duck source NDV, duck plague virus, the colloidal-gold detecting-card of duck circovirus three have following
Advantage：
Duck source of the present utility model NDV, duck plague virus, the colloidal-gold detecting-card of duck circovirus three, for duck
Source NDV, duck plague virus, duck circovirus antibody are detected, and any of which virus infection can be detected successfully,
Mixed infection detection can more be carried out.During detection, required sample size is few, kit it is easy to operate, easy to detect it is quick,
Detection sample is easily obtained, and immune response specificity is high, and result judgement is convenient.
Brief description of the drawings
Fig. 1 is duck source of the present utility model NDV, duck plague virus, the colloidal-gold detecting-card of duck circovirus three
Internal structure schematic diagram.
Fig. 2 is duck source of the present utility model NDV, duck plague virus, the colloidal-gold detecting-card of duck circovirus three
Surface structure schematic diagram.
Fig. 3 is duck source of the present utility model NDV, duck plague virus, the inspection of the colloidal-gold detecting-card of duck circovirus three
Survey the result schematic diagram of embodiment.
Fig. 4 is that duck source of the present utility model NDV, duck plague virus, the colloidal-gold detecting-card of duck circovirus three are another
The testing result schematic diagram of one detection embodiment.
Embodiment
Following examples are used to illustrate the utility model, but are not limited to the scope of the utility model.
As depicted in figs. 1 and 2, the utility model provides a kind of duck source NDV, duck plague virus, duck circovirus three
Join colloidal-gold detecting-card, gold-immunochromatographyreagent reagent for assay box includes test strips 3, loading pad 1, standard gold pad 2 and blotting paper 4, test strips 3
One end above stacked loading pad 1, standard gold pad 2, the other end of test strips 3 is stacked with blotting paper 4, and the bottom surface of test strips 3 is lining
Plate 5；Test strips 3 are coated with the first detection line 31 of duck source NDV from upper end, are coated with the second inspection of duck plague virus successively
Survey line 32, the 3rd detection line 33 and nature controlling line 34 for being coated with duck circovirus cap albumen, wherein, nature controlling line is coated with goat-anti
Mouse IgG.The anti-duck IgG antibody of mouse of colloid gold label is coated with standard gold pad 2.Duck source of the present utility model NDV, duck
Pestivirus, the colloidal-gold detecting-card of duck circovirus antibody three, it is only necessary to the sampling of duck blood once, pass through a detection
Card, it is possible to which viral mixed infection detects to these three, and the time of detection is short, three collaurums inspection of the present utility model
Card is surveyed, it is easy to operation, gained time for the timely diagnosis of disease, treatment, improve cure rate, reduce raiser's loss.
Wherein, collaurum is 38-42nm spheric granules.Test strips 3, loading pad 1 and standard gold pad 2 are fine using nitric acid
Tie up film.
Gold-immunochromatographyreagent reagent for assay box also includes buckle box, and the buckle box includes base 8 and lid 9, test strips 3, loading pad
1st, standard gold pad 2 and blotting paper 4 are fixed in box body by buckle box.One end of the corresponding loading pad of lid 9 offers circle
Loading hole 6, rectangular form 7 is offered at the detection line and nature controlling line of corresponding test strips, can be with by rectangular form 7
It was observed that the testing result of three gold-immunochromatographyreagent reagent for assay boxes.
Specifically, the radius in the loading hole of buckle box cover 9 is 0.15-0.35cm, wherein, a length of 11- of buckle box
12cm, a width of 2-4cm, thickness 0.2-0.4cm.The length, the buckle of width and the length and width for just adapting to test strips,
And make it that detection line, the length of nature controlling line are shorter, save the raw materials such as antigen-antibody.
Triplet gold-immunochromatographyreagent reagent for assay box application immunochromatography principle of the present utility model, takes blood serum sample to be checked, will
Blood serum sample to be checked is added in loading hole, and blood serum sample to be checked flows through gold standard pad by loading pad, if anti-duck source in serum be present
NDV, duck plague virus, the antibody of duck circovirus cap albumen, the antibody in serum can be with coated glue on standard gold pad
The anti-duck IgG antibody of body gold mark mouse combines, and conjugate may proceed to flow along test strips, and respectively with the coating duck in test strips
First detection line of source NDV, the second detection line for being coated with duck plague virus and coating duck circovirus cap albumen the
Three detection lines combine, and this three detections line shows red, and the anti-duck IgG antibody of mouse of last colloid gold label is run into test strips
Nature controlling line when, coated sheep anti-mouse igg antibody is combined with the anti-duck IgG antibody of mouse on nature controlling line, and display is red.Detect serum
In containing anti-duck source NDV, duck plague virus, the antibody of duck circovirus cap albumen.
If anti-duck source NDV, duck plague virus, the antibody of duck circovirus cap albumen are not contained in serum, no
It can be combined with the anti-duck IgG antibody of mouse, then the antibody in serum also would not be with coated duck source NDV, duck in detection line
Pestivirus, duck circovirus cap protein bindings, do not develop the color in detection line, it was demonstrated that without duck source NDV, duck plague disease in serum
Poison, duck circovirus cap protein antibodies.By the rectangular form hole on buckle box, the colour developing of observable detection line with
It is no, it can be determined that whether duck has infected duck source NDV, duck plague virus, duck circovirus.Three card of the present utility model
Detection kit has high specific and high sensitivity, is not required to carry any instrument and equipment, simple to operate, without professional,
It can obtain testing result within 15 minutes or so.
When duck source of the present utility model NDV, duck plague virus, the detection of the colloidal-gold detecting-card of duck circovirus three
Also need to dropper 1 to wrap, 1 bottle of sample buffer and 1 pair, disposable sterilized gloves.During detection, it is added dropwise with dropper into loading hole
Testing liquid, most suitable sample-adding amount is 50-150 μ l, preferably, sample-adding amount is 100 μ l.
Take serum two to be checked to drip with dropper, and be added dropwise in sample buffer and be well mixed, during loading, existed with dropper
Above-mentioned mixed liquor four is instilled in well to drip.After loading, detection reagent is stuck in placement 10-15 minutes at room temperature.Then to knot
Fruit is judged, negative：Without color reaction in detection line, do not develop the color, red reaction is shown on nature controlling line.It is positive：First detection
Line, the second detection line, the display of at least one detection line is red in the 3rd detection line, the red reaction of nature controlling line display.
As shown in figure 3, it is the utility model duck source NDV, duck plague virus, the inspection of the collaurum of duck circovirus three
When survey card detects to serum, contain duck source NDV antibody, antibody against duck plague virus, duck circovirus cap in serum
The result schematic diagram of detection, it is positive reaction, it was demonstrated that duck has infected duck source NDV antibody, duck simultaneously during protein antibodies
Pestivirus antibody, duck circovirus.
As shown in figure 4, it is the utility model duck source NDV, duck plague virus, the inspection of the collaurum of duck circovirus three
When survey card detects to serum, duck source NDV antibody, antibody against duck plague virus, duck circovirus are not contained in serum
During cap protein antibodies, the result schematic diagram of detection, the testing result proves that duck is uninfected by duck source NDV, duck plague disease
Poison, duck circovirus.
Duck source NDV, duck plague virus, duck circovirus infection are duck group common infection virus, and duck once infects
Virus is stated, great loss will be brought to raiser, the utility model utilizes the duck source of colloidal gold immunochromatographimethod technology preparation new
City epidemic disease poison, duck plague virus, the colloidal-gold detecting-card of duck circovirus three, can detect these three common virus, the inspection simultaneously
Test agent box is easy to operate, can substantially reduce the operating time.To duck source NDV, duck plague virus, duck circovirus antibody
Detected in time, any virus infection can be detected successfully, can more carry out mixed infection detection.During detection, institute
Need sample size few, kit it is easy to operate, convenient and swift, detection sample is easily obtained, and immune response specificity is high, as a result sentences
It is fixed convenient.
Although above having made detailed description to the utility model with generality explanation and specific embodiment,
On the basis of the utility model, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, the these modifications or improvements on the basis of without departing from the utility model spirit, belonging to the utility model will
Seek the scope of protection.
Claims (8)
1. a kind of duck source NDV, duck plague virus, duck circovirus colloidal-gold detecting-card, it is characterised in that the colloid
Gold detection card includes test strips, loading pad, standard gold pad and blotting paper, and the top at the loading end of test strips is stacked the loading
Pad, standard gold pad, the other end of test strips are stacked with absorbent filter, and the bottom surface of the test strips is PVC bottom plates；
The test strips are coated with the first detection line of duck source NDV, are coated with duck plague virus successively since loading end
Second detection line, the 3rd detection line and nature controlling line for being coated with duck circovirus cap albumen；
The anti-duck IgG antibody of mouse of colloid gold label is coated with the standard gold pad.
2. duck source according to claim 1 NDV, duck plague virus, duck circovirus colloidal-gold detecting-card, it is special
Sign is,
The nature controlling line is coated with sheep anti-mouse igg.
3. duck source according to claim 1 NDV, duck plague virus, duck circovirus colloidal-gold detecting-card, it is special
Sign is,
The collaurum is 38-42nm spheric granules.
4. duck source according to claim 1 NDV, duck plague virus, duck circovirus colloidal-gold detecting-card, it is special
Sign is,
The test strips, loading pad and standard gold pad are nitrocellulose membrane.
5. duck source according to claim 1 NDV, duck plague virus, duck circovirus colloidal-gold detecting-card, it is special
Sign is, the colloidal-gold detecting-card also includes buckle box, and the buckle box includes base and lid, the test strips, loading pad,
Standard gold pad and blotting paper are fixed by buckle box.
6. duck source according to claim 5 NDV, duck plague virus, duck circovirus colloidal-gold detecting-card, it is special
Sign is that one end that the lid corresponds to the loading pad offers circular loading hole, the detection line and matter of corresponding test strips
Rectangular form is offered at control line.
7. duck source according to claim 6 NDV, duck plague virus, duck circovirus colloidal-gold detecting-card, it is special
Sign is that the radius in the loading hole is 0.15-0.35cm.
8. duck source according to claim 6 NDV, duck plague virus, duck circovirus colloidal-gold detecting-card, it is special
Sign is, a length of 11-12cm of the buckle box, a width of 2-4cm, thickness 0.2-0.4cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720248195.0U CN206756847U (en) | 2017-03-14 | 2017-03-14 | Duck source NDV, duck plague virus, duck circovirus colloidal-gold detecting-card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720248195.0U CN206756847U (en) | 2017-03-14 | 2017-03-14 | Duck source NDV, duck plague virus, duck circovirus colloidal-gold detecting-card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206756847U true CN206756847U (en) | 2017-12-15 |
Family
ID=60609149
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720248195.0U Expired - Fee Related CN206756847U (en) | 2017-03-14 | 2017-03-14 | Duck source NDV, duck plague virus, duck circovirus colloidal-gold detecting-card |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206756847U (en) |
-
2017
- 2017-03-14 CN CN201720248195.0U patent/CN206756847U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171215 Termination date: 20190314 |