CN206706109U - Tissue Culture Dish perfusion device - Google Patents

Tissue Culture Dish perfusion device Download PDF

Info

Publication number
CN206706109U
CN206706109U CN201720422229.3U CN201720422229U CN206706109U CN 206706109 U CN206706109 U CN 206706109U CN 201720422229 U CN201720422229 U CN 201720422229U CN 206706109 U CN206706109 U CN 206706109U
Authority
CN
China
Prior art keywords
drain pipe
culture dish
screw thread
tissue culture
asepwirator pump
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201720422229.3U
Other languages
Chinese (zh)
Inventor
武昊
张华�
刘备
孙杨
李焕发
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fourth Military Medical University FMMU
Original Assignee
Fourth Military Medical University FMMU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fourth Military Medical University FMMU filed Critical Fourth Military Medical University FMMU
Priority to CN201720422229.3U priority Critical patent/CN206706109U/en
Application granted granted Critical
Publication of CN206706109U publication Critical patent/CN206706109U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model discloses Tissue Culture Dish perfusion device, including locating ring, feed tube, first asepwirator pump, first turbine flowmeter, first regulating valve, drain pipe, second asepwirator pump, second turbine flowmeter, second regulating valve, the Tissue Culture Dish perfusion device, clever structure, it is powerful, it is simple to operate, by using the device, it ensure that the stability of liquid flow direction, avoid pour into and flow out conduit mouth localized liquid flow is inconsistent and generation phenomena such as the generation and nutrient solution that cause to change the not thorough phenomenon of liquid are dried up, greatly improve the conventional efficient of medical personnel.

Description

Tissue Culture Dish perfusion device
Technical field
It the utility model is related to perfusion device, more particularly to Tissue Culture Dish perfusion device.
Background technology
At present, it is excessively simple for the perfusion mode of traditional cell culture, in use, medical personnel are in culture dish first Two conduits of middle placement, one pours into new nutrient solution, and another draws old nutrient solution.Such processing mode not only operates Trouble, conduit are fixed unstable, and it is inconsistent easily to occur pouring into and flow out conduit mouth localized liquid flow, causes to change liquid not Thoroughly phenomenon occurs, and has had a strong impact on the conventional efficient of cell culture perfusion, in view of disadvantages described above, is necessary to design cell in fact Culture dish perfusion device.
Utility model content
Technical problem to be solved in the utility model is:Tissue Culture Dish perfusion device is provided, to solve to pass at present The cell culture perfusion of system is excessively simple, the problem of reducing medical personnel's conventional efficient.
In order to solve the above technical problems, the technical solution of the utility model is:Tissue Culture Dish perfusion device, including positioning Ring, feed tube, the first asepwirator pump, the first turbine flowmeter, the first regulating valve, drain pipe, the second asepwirator pump, the second turbine flow Meter, the second regulating valve, described feed tube are located on the left of locating ring, and described feed tube is connected with locating ring screw thread, described First asepwirator pump is located on the left of feed liquor pipe outer wall, and the first described asepwirator pump is connected with feed liquor pipe screw thread, the first described turbine Flowmeter is located at the top of feed tube, and the first described turbine flowmeter is connected with feed liquor pipe screw thread, the first described regulating valve position On the left of the first turbine flowmeter, the first described regulating valve is connected with feed liquor pipe screw thread, and described drain pipe is located at locating ring Right side, described drain pipe are connected with locating ring screw thread, and the second described asepwirator pump is located on the right side of drain pipe outer wall, and described the Two asepwirator pumps are connected with drain pipe screw thread, and the second described turbine flowmeter is located at the top of drain pipe, the second described turbine flow Gauge is connected with drain pipe screw thread, and the second described regulating valve is located on the left of the second turbine flowmeter, the second described regulating valve It is connected with drain pipe screw thread.
Further, described feed tube inner right side is also equipped with a number of inlet opening, and described inlet opening is circle Shape through hole.
Further, described drain pipe inside left is also equipped with a number of fluid hole, and described fluid hole is circle Shape through hole.
Further, described drain pipe top left side is additionally provided with connecting rod, and described connecting rod is connected with drain pipe screw thread.
Further, described connecting rod outer upper ends have also extended through magnifying glass, and described magnifying glass rotates phase with connecting rod Even.
Further, described magnifying glass top right side is additionally provided with the first location-plate, the first described location-plate and magnifying glass It is rotatedly connected, and the first described location-plate is connected with connecting rod close-fitting.
Further, described magnifying glass bottom right is additionally provided with the second location-plate, the second described location-plate and magnifying glass It is rotatedly connected, and the second described location-plate is connected with connecting rod close-fitting.
Compared with prior art, Tissue Culture Dish perfusion device, before use, first in the presence of locating ring so that Feed tube and drain pipe are connected as a single entity, and described locating ring is transparent material, in use, medical personnel first by the first asepwirator pump, Second asepwirator pump, the first turbine flowmeter and the second turbine flowmeter are connected with extraneous power supply, and medical personnel hold this again Device, then feed tube and drain pipe are positioned in culture dish so that locating ring is in the surface of culture dish, and medical personnel are again The first asepwirator pump is opened with hand and nutrient solution is discharged at inlet opening by the second asepwirator pump, the first asepwirator pump from feed tube, then by entering Nutrient solution is discharged into culture dish by fluid apertures, and in the course of work of the first asepwirator pump, the first turbine flowmeter in feed tube to training The flow of nutrient solution is monitored, and medical personnel can know to cultivate in current feed tube by the numerical value that the first turbine flowmeter is shown The specific traffic conditions of liquid, when the numerical value shown by the first turbine flowmeter is not up to the demand of medical personnel, medical personnel The flow of nutrient solution in feed tube can be controlled by using the first regulating valve, with this so that the first turbine flowmeter monitors Numerical value change, after the numerical value that the first turbine flowmeter is shown reaches the demand of medical personnel, medical personnel stop make With the first regulating valve, meanwhile, by opening the second asepwirator pump so that the nutrient solution in culture dish is discharged into out liquid by fluid hole In pipe, then next procedure is discharged into by drain pipe, in the course of work of the second asepwirator pump, the second turbine flowmeter is in drain pipe The flow of nutrient solution is monitored, and medical personnel can know to train in current drain pipe by the numerical value that the second turbine flowmeter is shown The specific flow of nutrient solution, when the numerical value shown by the second turbine flowmeter is not up to the demand of medical personnel, medical personnel can The flow of nutrient solution in drain pipe is controlled by using the second regulating valve, with this so that what the second turbine flowmeter monitored Numerical value changes, and after the numerical value that the second turbine flowmeter is shown reaches the demand of medical personnel, medical personnel stop using Second regulating valve, the Tissue Culture Dish perfusion device, clever structure is powerful, simple to operate, by using the dress Put, it is ensured that the stability of liquid flow direction, avoid that to pour into and flow out conduit mouth localized liquid flow inconsistent and cause Change the generation of the not thorough phenomenon of liquid and generation phenomena such as nutrient solution dries up, greatly improve the experiment of medical personnel Efficiency, meanwhile, according to actual use situation, medical personnel can hand-hold magnifying glass, in the work that magnifying glass and connecting rod are rotatedly connected Under so that magnifying glass is rotated to the surface of culture dish, by using magnifying glass, facilitates the more detailed observation of medical personnel The concrete condition of nutrient solution perfusion in culture dish, the first location-plate and the second location-plate are to prevent magnifying glass when rotated And moved up and down along the direction of connecting rod, it is ensured that the normality that magnifying glass uses, the first described turbine flowmeter and Two turbine flowmeter model DN20.
Brief description of the drawings
Fig. 1 is the partial front elevation view of Tissue Culture Dish perfusion device;
Fig. 2 is the inside top sectional view of feed tube and drain pipe.
Locating ring 1, feed tube 2, the first asepwirator pump 3, the first turbine flowmeter 4, the first regulating valve 5, drain pipe 6, second Asepwirator pump 7, the second turbine flowmeter 8, the second regulating valve 9, inlet opening 201, fluid hole 601, connecting rod 602, magnifying glass 603, First location-plate 604, the second location-plate 605.
Following embodiment will combine above-mentioned accompanying drawing and further illustrate.
Embodiment
Hereinafter, a variety of specific details are elaborated, to provide the saturating of the concept to forming described embodiment basis Thorough understanding.However, it will be apparent to those skilled in the art that described embodiment can be in these no specific details In it is some or all in the case of put into practice.In other cases, well-known processing step is not specifically described.
As shown in Figure 1 and Figure 2, Tissue Culture Dish perfusion device, including locating ring 1, feed tube 2, the first asepwirator pump 3, first Turbine flowmeter 4, the first regulating valve 5, drain pipe 6, the second asepwirator pump 7, the second turbine flowmeter 8, the second regulating valve 9, it is described Feed tube 2 be located at 1 on the left of locating ring, described feed tube 2 is connected with the screw thread of locating ring 1, and the first described asepwirator pump 3 is located at On the left of the outer wall of feed tube 2, the first described asepwirator pump 3 is connected with the screw thread of feed tube 2, and the first described turbine flowmeter 4 is located at The top of feed tube 2, the first described turbine flowmeter 4 are connected with the screw thread of feed tube 2, and the first described regulating valve 5 is located at first The left side of turbine flowmeter 4, the first described regulating valve 5 are connected with the screw thread of feed tube 2, and it is right that described drain pipe 6 is located at locating ring 1 Side, described drain pipe 6 are connected with the screw thread of locating ring 1, and the second described asepwirator pump 7 is located on the right side of the outer wall of drain pipe 6, described Second asepwirator pump 7 is connected with the screw thread of drain pipe 6, and the second described turbine flowmeter 8 is located at the top of drain pipe 6, and described second Turbine flowmeter 8 is connected with the screw thread of drain pipe 6, and the second described regulating valve 9 is located at the left side of the second turbine flowmeter 8, described Second regulating valve 9 is connected with the screw thread of drain pipe 6, and the described inner right side of feed tube 2 is also equipped with a number of inlet opening 201, described inlet opening 201 is manhole, and the described inside left of drain pipe 6 is also equipped with a number of fluid hole 601, described fluid hole 601 is manhole, and the described top left side of drain pipe 6 is additionally provided with connecting rod 602, described connection Bar 602 is connected with drain pipe screw thread, and the described outer upper ends of connecting rod 602 have also extended through magnifying glass 603, described magnifying glass 603 are rotatedly connected with connecting rod 602, and the described top right side of magnifying glass 603 is additionally provided with the first location-plate 604, and described first Location-plate 604 is rotatedly connected with magnifying glass 603, and the first described location-plate 604 is connected with the close-fitting of connecting rod 602, described The bottom right of magnifying glass 603 is additionally provided with the second location-plate 605, and the second described location-plate 605 is rotatedly connected with magnifying glass 603, And the second described location-plate 605 is connected with the close-fitting of connecting rod 602.
The Tissue Culture Dish perfusion device, before use, first in the presence of locating ring 1 so that feed tube 2 and drain pipe 6 are connected as a single entity, and described locating ring 1 is transparent material, in use, medical personnel first by the first asepwirator pump 3, the second asepwirator pump 7, First turbine flowmeter 4 and the second turbine flowmeter 8 are connected with extraneous power supply, and medical personnel hold the device again, then will Feed tube 2 and drain pipe 6 are positioned in culture dish so that locating ring 1 is in the surface of culture dish, and medical personnel are opened with hand again The first asepwirator pump 3 and the second asepwirator pump 7 are opened, nutrient solution is discharged at inlet opening 201 by the first asepwirator pump 3 from feed tube 2, then by entering Nutrient solution is discharged into culture dish by fluid apertures 201, and in the course of work of the first asepwirator pump 3, the first turbine flowmeter 4 is to feed tube The flow of nutrient solution is monitored in 2, and medical personnel can know current feed tube by the numerical value that the first turbine flowmeter 4 is shown The specific traffic conditions of nutrient solution in 2, when the numerical value shown by the first turbine flowmeter 4 is not up to the demand of medical personnel, Medical personnel can be controlled by using the first regulating valve 5 to the flow of nutrient solution in feed tube 2, with this so that the first turbine The numerical value that flowmeter 4 monitors changes, after the numerical value of the first turbine flowmeter 4 display reaches the demand of medical personnel, doctor Shield personnel stop using the first regulating valve 5, meanwhile, pass through and open the second asepwirator pump 7 so that nutrient solution in culture dish by Fluid hole 601 is discharged into drain pipe 6, then is discharged into next procedure by drain pipe 6, in the course of work of the second asepwirator pump 7, second Turbine flowmeter 8 is monitored to the flow of nutrient solution in drain pipe 6, and medical personnel can be shown by the second turbine flowmeter 8 Numerical value know the specific flow of nutrient solution in current drain pipe 6, when the numerical value shown by the second turbine flowmeter 8 is not up to cured During the demand of shield personnel, medical personnel can be controlled by using the second regulating valve 9 to the flow of nutrient solution in drain pipe 6, With this so that the numerical value of the second turbine flowmeter 8 monitoring changes, when the numerical value of the second turbine flowmeter 8 display reaches medical care After the demand of personnel, medical personnel stop using the second regulating valve 9, meanwhile, according to actual use situation, medical personnel can Hand-hold magnifying glass 603, in the presence of magnifying glass 603 and connecting rod 602 are rotatedly connected so that the rotation of magnifying glass 603 to culture The surface of ware, by using magnifying glass 603, facilitate the more detailed observation nutrient solution perfusion in culture dish of medical personnel Concrete condition, the first location-plate 604 and the second location-plate 605 be in order to prevent magnifying glass 603 when rotated and along connecting rod 602 direction moves up and down, it is ensured that the normality that magnifying glass 603 uses, the first described turbine flowmeter 4 and the second turbine The model DN20 of flowmeter 8.
The utility model is not limited to above-mentioned specific embodiment, and one of ordinary skill in the art visualizes from above-mentioned Hair, without performing creative labour, a variety of conversion made, all falls within the scope of protection of the utility model.

Claims (7)

1. Tissue Culture Dish perfusion device, it is characterised in that including locating ring, feed tube, the first asepwirator pump, the first turbine flow Meter, the first regulating valve, drain pipe, the second asepwirator pump, the second turbine flowmeter, the second regulating valve, described feed tube is positioned at fixed On the left of the ring of position, described feed tube is connected with locating ring screw thread, and the first described asepwirator pump is located on the left of feed liquor pipe outer wall, described The first asepwirator pump be connected with feed liquor pipe screw thread, the first described turbine flowmeter is located at the top of feed tube, the first described whirlpool Flowmeters are connected with feed liquor pipe screw thread, and the first described regulating valve is located on the left of the first turbine flowmeter, and described first adjusts Section valve is connected with feed liquor pipe screw thread, and described drain pipe is located on the right side of locating ring, and described drain pipe is connected with locating ring screw thread, The second described asepwirator pump is located on the right side of drain pipe outer wall, and the second described asepwirator pump is connected with drain pipe screw thread, and described the Two turbine flowmeters are located at the top of drain pipe, and the second described turbine flowmeter is connected with drain pipe screw thread, and described second adjusts Section valve is located on the left of the second turbine flowmeter, and the second described regulating valve is connected with drain pipe screw thread.
2. Tissue Culture Dish perfusion device as claimed in claim 1, it is characterised in that described feed tube inner right side is also equal Provided with a number of inlet opening, described inlet opening is manhole.
3. Tissue Culture Dish perfusion device as claimed in claim 2, it is characterised in that described drain pipe inside left is also equal Provided with a number of fluid hole, described fluid hole is manhole.
4. Tissue Culture Dish perfusion device as claimed in claim 3, it is characterised in that described drain pipe top left side is also set There is connecting rod, described connecting rod is connected with drain pipe screw thread.
5. Tissue Culture Dish perfusion device as claimed in claim 4, it is characterised in that described connecting rod outer upper ends are also passed through Magnifying glass is installed with, described magnifying glass is rotatedly connected with connecting rod.
6. Tissue Culture Dish perfusion device as claimed in claim 5, it is characterised in that described magnifying glass top right side is also set There is the first location-plate, the first described location-plate is rotatedly connected with magnifying glass, and the first described location-plate and connecting rod close-fitting It is connected.
7. Tissue Culture Dish perfusion device as claimed in claim 6, it is characterised in that described magnifying glass bottom right is also set There is the second location-plate, the second described location-plate is rotatedly connected with magnifying glass, and the second described location-plate and connecting rod close-fitting It is connected.
CN201720422229.3U 2017-04-21 2017-04-21 Tissue Culture Dish perfusion device Expired - Fee Related CN206706109U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720422229.3U CN206706109U (en) 2017-04-21 2017-04-21 Tissue Culture Dish perfusion device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720422229.3U CN206706109U (en) 2017-04-21 2017-04-21 Tissue Culture Dish perfusion device

Publications (1)

Publication Number Publication Date
CN206706109U true CN206706109U (en) 2017-12-05

Family

ID=60455592

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201720422229.3U Expired - Fee Related CN206706109U (en) 2017-04-21 2017-04-21 Tissue Culture Dish perfusion device

Country Status (1)

Country Link
CN (1) CN206706109U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112522081A (en) * 2020-12-28 2021-03-19 王艳青 Efficient cell culture dish perfusion device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112522081A (en) * 2020-12-28 2021-03-19 王艳青 Efficient cell culture dish perfusion device
CN112522081B (en) * 2020-12-28 2022-09-27 上海揽微赛尔生物科技有限公司 Cell culture dish perfusion device

Similar Documents

Publication Publication Date Title
CN205694768U (en) Novel water conservancy irrigation rig
CN206706109U (en) Tissue Culture Dish perfusion device
CN201528584U (en) Hatching barrel device without blind angle flowing water
CN204119907U (en) Realize the mixing arrangement of irrigating spray medicine fertilizing integrated
CN206482898U (en) A kind of hemodialysis system
CN208024993U (en) Flat section is flow automatically water-bound under a kind of pressure pipeline
CN207927223U (en) A kind of manaca seedling cultivation irrigation device
CN205812785U (en) It is applicable to the fertile machine of portable molten fertile note of surface irrigation water-fertilizer integral
CN203483701U (en) Dialyzate stirring and preparing machine
CN105993354B (en) Fertile machine is noted suitable for the portable molten fertilizer of surface irrigation water-fertilizer integral
CN206027566U (en) Inner loop stirring formula hemodialysis solutions configuration device
CN107574121A (en) A kind of Tissue Culture Dish perfusion device
CN209160180U (en) Online filling buffer decontamination system
CN108308004A (en) The built-in sprinkler with reel of cylindrantherae
CN207551990U (en) A kind for the treatment of device for oil field well washing acidifying waste liquid
CN206770672U (en) Three-way valve used for aquiculture
CN221601885U (en) Sewage treatment medicament processing raw materials adder
CN206384877U (en) Sewage disposal Aerobic Pond bubble elimination device
CN204182618U (en) A kind of continuous-flow type automatic corn cleaning machine
CN207894480U (en) A kind of rotary drum liquid level capacity display device
CN212338281U (en) Binary channels instrument valve
CN206492735U (en) Elisa plate board-washing device
CN204220121U (en) Emulsion polymerisation initator dissolves and drips tank
CN206676245U (en) A kind of molten medicine-chest suitable for low temperature environment
CN207827097U (en) A kind of nursing waste collecting device

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20171205

Termination date: 20180421

CF01 Termination of patent right due to non-payment of annual fee