CN206706096U - A kind of beet Pseudoperonospora cubensis specific PCR detection kit - Google Patents
A kind of beet Pseudoperonospora cubensis specific PCR detection kit Download PDFInfo
- Publication number
- CN206706096U CN206706096U CN201621140743.XU CN201621140743U CN206706096U CN 206706096 U CN206706096 U CN 206706096U CN 201621140743 U CN201621140743 U CN 201621140743U CN 206706096 U CN206706096 U CN 206706096U
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- China
- Prior art keywords
- supporting plate
- bottle
- beet
- outer box
- detection kit
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- 235000016068 Berberis vulgaris Nutrition 0.000 title claims abstract description 21
- 241000335053 Beta vulgaris Species 0.000 title claims abstract description 21
- 241001281805 Pseudoperonospora cubensis Species 0.000 title claims abstract description 20
- 238000001514 detection method Methods 0.000 title claims abstract description 20
- 230000000692 anti-sense effect Effects 0.000 claims abstract description 9
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 9
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims abstract description 8
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 7
- 239000013641 positive control Substances 0.000 claims abstract description 7
- 239000013642 negative control Substances 0.000 claims abstract description 6
- 239000000523 sample Substances 0.000 claims abstract description 6
- 239000012154 double-distilled water Substances 0.000 claims abstract description 4
- 229910001629 magnesium chloride Inorganic materials 0.000 claims abstract description 4
- 239000006260 foam Substances 0.000 claims description 12
- 238000007373 indentation Methods 0.000 claims description 3
- 238000011144 upstream manufacturing Methods 0.000 claims 1
- 201000010099 disease Diseases 0.000 abstract description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 13
- 244000052769 pathogen Species 0.000 abstract description 6
- 230000001717 pathogenic effect Effects 0.000 abstract description 6
- 238000003745 diagnosis Methods 0.000 abstract description 5
- 235000011147 magnesium chloride Nutrition 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 206010027146 Melanoderma Diseases 0.000 description 2
- 235000014443 Pyrus communis Nutrition 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 235000005940 Centaurea cyanus Nutrition 0.000 description 1
- 240000004385 Centaurea cyanus Species 0.000 description 1
- 241001582888 Lobus Species 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002872 contrast media Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000039328 opportunistic pathogen Species 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses a kind of beet Pseudoperonospora cubensis specific PCR detection kit, and it includes outer box and internal box, is provided with the first supporting plate and the second supporting plate in internal box, the first supporting plate in the second supporting plate with being respectively equipped with：Positive control agent bottle, negative control reagent bottle, sense primer bottle, anti-sense primer bottle, Tap Man probes bottle, DNA profiling bottle, magnesium chloride solution bottle, 10 ddH2O bottles, a side of outer box are provided with lug boss, and another side corresponding with lug boss is provided with groove；End face is provided with two stopper slots to cave at the top of outer box, and the bottom of outer box is provided with two limited blocks, is placed on by the limited block of an outer box in the stopper slot of another outer box, several outer boxes are stacked together.The utility model can carry out quick diagnosis and pathogen identification, timely and effectively carry out disease control, control disease, reduce economic loss.
Description
Technical field
It the utility model is related to kit field, and in particular to a kind of beet Pseudoperonospora cubensis specific PCR detection reagent
Box.
Background technology
Beet Pseudoperonospora cubensis is important plant quarantine venereal disease opportunistic pathogen, Pseudoperonospora cubensis is mainly invaded to the very harmful of beet
Contaminate the tender organ of beet children, lobus cardiacus of causing harm more, the blade of bacteria infection is initially light green color, after be changed into yellow until there is brown
Irregular shape scab.
Directly observed using traditional plant disease detection method, the symptom feelings that early stage is not exhibited by can be omitted
Condition, and this detection method relies on morphology, is easily disturbed by human factor and environmental condition, in addition traditional classification
Method it is time-consuming it is longer, program is cumbersome, the requirement of quick detection is unable to reach, it is difficult to monitor propagation and the disease stream of pathogen in time
OK.Therefore, with the development of molecular biology, by constantly obtaining the gene of beet Pseudoperonospora cubensis, different reagents is prepared
Box, quick diagnosis and pathogen identification can be carried out, timely and effectively carry out disease control, control disease, reduce economic loss.
The content of the invention
The purpose of this utility model is to overcome problem above existing for prior art, there is provided a kind of beet Pseudoperonospora cubensis is special
Different in nature PCR detection kit, the utility model can carry out quick diagnosis and pathogen identification, timely and effectively carry out disease and prevent
Control, control disease, reduce economic loss.
To realize above-mentioned technical purpose and the technique effect, the utility model is achieved through the following technical solutions：
A kind of beet Pseudoperonospora cubensis specific PCR detection kit, it includes outer box and is arranged on the outer box
The internal box in internal portion, the opening of the internal box are provided with lid, be provided with the internal box the first supporting plate with
Second supporting plate, first supporting plate in the second supporting plate with being respectively equipped with：Positive control agent bottle, negative control reagent
Bottle, sense primer bottle, anti-sense primer bottle, Tap Man probes bottle, DNA profiling bottle, magnesium chloride solution bottle, 10 ddH2O bottles, institute
The side for stating outer box is provided with lug boss, and another side corresponding with the lug boss is provided with groove, by described convex
The portion of rising coordinates with the groove is bolted together several described outer boxes；End face is provided with two at the top of the outer box
The individual stopper slot to cave in, the bottom of the outer box are provided with two limited blocks, pass through the limited block of an outer box
It is placed in the stopper slot of another outer box, several outer boxes is stacked together.
It is further preferred that the side of the first supporting plate two is equipped with hand-strap, one end of the hand-strap with it is described
Fixed between first supporting plate, the other end of the hand-strap is arranged on above first supporting plate, by described portable
First supporting plate is proposed the internal box by band.
It is further preferred that two sides of second supporting plate are equipped with hand-strap, one end of the hand-strap and institute
State fixed between the second supporting plate, the other end of the hand-strap is arranged on above second supporting plate, passes through the hand
Second supporting plate is proposed the internal box by belt.
It is further preferred that first supporting plate and second supporting plate are cystosepiment or plastic plate.
It is further preferred that the first foam pad is provided between first supporting plate and second supporting plate, described
One foam pad is arranged between the bottle on the bottle in first supporting plate and second supporting plate.
It is further preferred that also including the second foam pad, second foam pad is arranged on the bottom of the internal box.
It is further preferred that bonded between the lid and the internal box using hasp or magnet.
It is further preferred that it is additionally provided with semicircular indentations on the lid.
It is further preferred that
Sense primer in the sense primer bottle is：5’ -ATGAAATAATGGCACGCTCC-3’.
It is further preferred that
Anti-sense primer in the anti-sense primer bottle is：5’ -GATGCTGGCTTATGTGCTGA-3’.
The beneficial effects of the utility model are:
The utility model can carry out quick diagnosis and pathogen identification, timely and effectively carry out disease control, control disease
Evil, reduce economic loss.Kit of the present utility model uses double-layer box structure, and a side of outer box is provided with projection
Portion, another side corresponding with lug boss are provided with groove, are coordinated by lug boss and groove several outer boxes are assembled
Together；End face is provided with two stopper slots to cave at the top of outer box, and the bottom of outer box is provided with two limited blocks, leads to
The limited block for crossing an outer box is placed in the stopper slot of another outer box, and several outer boxes are overlayed into one
Rise.On the one hand internal box structure can be protected by the structure of said external box body, on the other hand can be by some kits
Discharge is together, i.e., conveniently laying, and firmly, avoids kit from toppling over.
Described above is only the general introduction of technical solutions of the utility model, in order to better understand skill of the present utility model
Art means, and being practiced according to the content of specification, with preferred embodiment of the present utility model and coordinate accompanying drawing detailed below
Describe in detail bright as after.Specific embodiment of the present utility model is shown in detail by following examples and its accompanying drawing.
Brief description of the drawings
In order to illustrate more clearly of the technical scheme in the utility model embodiment technology, embodiment technology will be retouched below
The required accompanying drawing used is briefly described in stating, it should be apparent that, drawings in the following description are only the utility model
Some embodiments, for those of ordinary skill in the art, on the premise of not paying creative work, can also basis
These accompanying drawings obtain other accompanying drawings.
Fig. 1 is external structure of the present utility model；
Fig. 2 is Fig. 1 top view；
Fig. 3 is the structural representation of internal box of the present utility model；
Fig. 4 is the top view of internal structure in Fig. 3；
Wherein, 10- outer boxes, 101- lug bosses, 102- grooves, 103- stopper slots, 104- limited blocks, 20- inner cartridges
Body, 201- lids, 202- half round cut, the supporting plates of 30- first, 301- positive control agent bottles, 302- negative control reagents
Bottle, 303- sense primer bottles, 304- anti-sense primer bottles, 305-Tap Man probe bottles, 306-DNA template bottles, 307- magnesium chlorides
Solution bottle, 308-ddH2O bottles.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belong to the scope of the utility model protection.
Embodiment
A kind of beet Pseudoperonospora cubensis specific PCR detection kit is disclosed in the present embodiment, it includes outer box 10
With the internal box 20 being arranged on inside said external box body 10, wherein, institute in the structure such as Fig. 1-2 of said external box body 10
Show, a side of said external box body 10 is provided with lug boss 101, and another side corresponding with above-mentioned lug boss 101 is provided with recessed
Groove 102, coordinated by above-mentioned lug boss 101 with above-mentioned groove 102 and several said external box bodys 10 are bolted together；On
State the top end face of outer box 10 and be provided with two stopper slots 103 to cave in, the bottom of said external box body 10 is provided with two limits
Position block 104, the stopper slot of another said external box body 10 is placed on by the limited block 104 of a said external box body 10
In 103, several outer boxes 10 are stacked together.
Two stopper slots 103 to cave in are provided with the top end face of said external box body 10, the bottom of outer box 10 is set
There are two limited blocks 104, the stopper slot of another outer box 10 is placed on by the limited block 104 of an outer box 10
In 103, several outer boxes 10 are stacked together.On the one hand inside can be protected by the structure of said external box body 10
Box body structure, on the other hand some kits can be discharged together, i.e., it is conveniently laying, and firmly, avoid kit from toppling over.
As shown in Figure 3-4, the opening of above-mentioned internal box 20 is provided with lid 201, above-mentioned lid 201 and above-mentioned inner cartridge
Bonded between body 20 using hasp or magnet, semicircular indentations 202 are additionally provided with above-mentioned lid 201.
The first supporting plate 30 and the second supporting plate, above-mentioned first supporting plate 30 and are provided with above-mentioned internal box 201
It is respectively equipped with two supporting plates：Positive control agent bottle 301, negative control reagent bottle 302, sense primer bottle 303, anti-sense primer
307,10 bottle 304, Tap Man probes bottle 305, DNA profiling bottle 306, magnesium chloride solution bottle ddH2O308 bottles.
Wherein, in the present embodiment, it is the DNA of Pear black spot extraction, above-mentioned feminine gender in above-mentioned positive control agent bottle 301
It is the DNA without Pear black spot sample extraction in contrast agents bottle 302, the wherein red bottle cap mark of positive control agent bottle 301
Show, negative control reagent bottle 302 is indicated with blue bottle cap.
Sense primer in above-mentioned sense primer bottle 303 is：5’ -ATGAAATAATGGCACGCTCC-3’；Above-mentioned downstream
Anti-sense primer in primer bottle 304 is： 5’-GATGCTGGCTTATGTGCTGA-3’.
In the present embodiment, because above-mentioned first supporting plate 30 and above-mentioned second supporting plate are cystosepiment or plastic plate.
Above-mentioned first supporting plate 30 and the inside for being stuck in internal box 20 of above-mentioned second supporting plate tightly, for convenience by above-mentioned support
Plate is taken out, and hand-strap, one end of above-mentioned hand-strap and above-mentioned first supporting plate are equipped with above-mentioned first supporting plate, 30 liang of sides
Fixed between 30, the other end of above-mentioned hand-strap is arranged on above above-mentioned first supporting plate 30, will be upper by above-mentioned hand-strap
State the first supporting plate 30 and propose above-mentioned tray interior.
Likewise, be equipped with above-mentioned hand-strap in two sides of above-mentioned second supporting plate, one end of above-mentioned hand-strap with it is upper
State fixed between the second supporting plate, the other end of above-mentioned hand-strap is arranged on above above-mentioned second supporting plate, passes through above-mentioned hand
Belt proposes above-mentioned second supporting plate inside above-mentioned internal box 20.
In order to protect two layers of reagent bottle, the first foam is provided between above-mentioned first supporting plate 30 and above-mentioned second supporting plate
Pad, above-mentioned first foam pad are arranged between the bottle on the bottle in above-mentioned first supporting plate 30 and above-mentioned second supporting plate.
Meanwhile the second foam pad is additionally provided with, above-mentioned second foam pad is arranged on the bottom of above-mentioned internal box 20.
The utility model can carry out quick diagnosis and pathogen identification, timely and effectively carry out disease control, control disease
Evil, reduce economic loss.Handle band structure is also provided with the utility model, between one end of hand-strap and the first supporting plate
Fixed, the other end of hand-strap is arranged on above the first supporting plate, and above-mentioned first supporting plate is proposed into box body by hand-strap
It is internal；Supporting plate is proposed into box body by the way that handle strip is convenient, it is easy to detect.
The foregoing description of the disclosed embodiments, professional and technical personnel in the field are enable to realize or new using this practicality
Type.A variety of modifications to these embodiments will be apparent for those skilled in the art, determine herein
The General Principle of justice can be realized in other embodiments in the case where not departing from spirit or scope of the present utility model.Cause
This, the utility model is not intended to be limited to the embodiments shown herein, and is to fit to and principles disclosed herein
The most wide scope consistent with features of novelty.
Claims (10)
1. a kind of beet Pseudoperonospora cubensis specific PCR detection kit, it includes outer box and is arranged on the outer box
Internal internal box, the opening of the internal box are provided with lid, provided with the first supporting plate and the in the internal box
Two supporting plates, first supporting plate in the second supporting plate with being respectively equipped with：Positive control agent bottle, negative control reagent bottle,
Sense primer bottle, anti-sense primer bottle, Tap Man probes bottle, DNA profiling bottle, magnesium chloride solution bottle, 10 ddH2O bottles, its feature
It is, a side of the outer box is provided with lug boss, and another side corresponding with the lug boss is provided with groove, passed through
The lug boss coordinates with the groove is bolted together several described outer boxes；At the top of the outer box on end face
Provided with two stopper slots to cave in, the bottom of the outer box is provided with two limited blocks, passes through outer box
Limited block is placed in the stopper slot of another outer box, and several outer boxes are stacked together.
2. beet Pseudoperonospora cubensis specific PCR detection kit according to claim 1, it is characterised in that described first
The side of supporting plate two is equipped with hand-strap, fixed between one end of the hand-strap and first supporting plate, the hand-strap
The other end be arranged on above first supporting plate, first supporting plate is proposed by the inside by the hand-strap
Box body.
3. beet Pseudoperonospora cubensis specific PCR detection kit according to claim 1, it is characterised in that described second
Two sides of supporting plate are equipped with hand-strap, fixed between one end of the hand-strap and second supporting plate, described portable
The other end of band is arranged on above second supporting plate, is proposed second supporting plate in described by the hand-strap
Portion's box body.
4. beet Pseudoperonospora cubensis specific PCR detection kit according to claim 1, it is characterised in that described first
Supporting plate and second supporting plate are cystosepiment or plastic plate.
5. beet Pseudoperonospora cubensis specific PCR detection kit according to claim 1, it is characterised in that described first
The first foam pad is provided between supporting plate and second supporting plate, first foam pad is arranged in first supporting plate
Bottle and second supporting plate on bottle between.
6. beet Pseudoperonospora cubensis specific PCR detection kit according to claim 1, it is characterised in that also including
Two foam pads, second foam pad are arranged on the bottom of the internal box.
7. beet Pseudoperonospora cubensis specific PCR detection kit according to claim 1, it is characterised in that the lid
Bonded between the internal box using hasp or magnet.
8. beet Pseudoperonospora cubensis specific PCR detection kit according to claim 1, it is characterised in that the lid
On be additionally provided with semicircular indentations.
9. beet Pseudoperonospora cubensis specific PCR detection kit according to claim 1, it is characterised in that the upstream
Sense primer in primer bottle is：5’-ATGAAATAATGGCACGCTCC-3’.
10. beet Pseudoperonospora cubensis specific PCR detection kit according to claim 1, it is characterised in that the downstream
Anti-sense primer in primer bottle is：5’-GATGCTGGCTTATGTGCTGA-3’.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621140743.XU CN206706096U (en) | 2016-10-20 | 2016-10-20 | A kind of beet Pseudoperonospora cubensis specific PCR detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621140743.XU CN206706096U (en) | 2016-10-20 | 2016-10-20 | A kind of beet Pseudoperonospora cubensis specific PCR detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206706096U true CN206706096U (en) | 2017-12-05 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621140743.XU Expired - Fee Related CN206706096U (en) | 2016-10-20 | 2016-10-20 | A kind of beet Pseudoperonospora cubensis specific PCR detection kit |
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| Country | Link |
|---|---|
| CN (1) | CN206706096U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110592258A (en) * | 2019-09-30 | 2019-12-20 | 黑龙江大学 | Method for identifying beet variety based on DAMD molecular marker |
-
2016
- 2016-10-20 CN CN201621140743.XU patent/CN206706096U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110592258A (en) * | 2019-09-30 | 2019-12-20 | 黑龙江大学 | Method for identifying beet variety based on DAMD molecular marker |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171205 |