CN203324277U - ELISA kit used for detecting porcine reproductive and respiratory syndrome antibodies - Google Patents
ELISA kit used for detecting porcine reproductive and respiratory syndrome antibodies Download PDFInfo
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- CN203324277U CN203324277U CN2013203794954U CN201320379495U CN203324277U CN 203324277 U CN203324277 U CN 203324277U CN 2013203794954 U CN2013203794954 U CN 2013203794954U CN 201320379495 U CN201320379495 U CN 201320379495U CN 203324277 U CN203324277 U CN 203324277U
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Abstract
The utility model provides an ELISA kit used for detecting porcine reproductive and respiratory syndrome antibodies. The ELISA kit used for detecting the porcine reproductive and respiratory syndrome antibodies comprises a kit body and a kit cover arranged on the kit body in a buckling mode, wherein a cavity suitable for containing an ice bag is formed in the middle of the kit body, seven cylindrical grooves are formed in the left side of the cavity, seven bottles of reagents are arranged in the seven cylindrical grooves respectively, a rectangular groove is formed in the right side of the cavity, and a 96-hole enzyme-labeled plate is arranged in the rectangular groove. The seven bottles of reagents are respectively a cleaning solution, a serum diluent, a chromogenic substrate, second rabbit anti-pig enzyme-labeled antibodies, a stop solution, positive serum and negative serum, and the 96-hole enzyme-labeled plate is wrapped by recombinant GP5 protein antigens of porcine reproductive and respiratory syndrome virus GP5 protein genes, and is sealed by bovine serum albumin. The ELISA kit used for detecting the porcine reproductive and respiratory syndrome antibodies is convenient to take and use and portable. The reagents are taken out for use when the ELISA kit is used, the reagents are prevented from being placed by mistake or forgotten to be placed, and the work efficiency of operators is improved.
Description
Technical field
The utility model belongs to technical field of medical instruments, particularly, relates to a kind of ELISA kit that detects porcine reproductive and respiratory syndrome antibody.
Background technology
In the medical diagnosis industry, often need to diagnose the state of an illness by the testing result of reagent.Reagent is all to be loaded in an airtight reagent bottle to preserve usually, not without other further safeguard measures.But from the angle of health and the angle of safety, such bulk storage mode is obviously irrational, and first reagent bottle is not protected again, is easy to occur leakage pollution; It two is that reagent bottle is not separately preserved after taking reagent, has the hidden danger of obscuring; It three is that reagent bottle does not store together with the paramedicines or the device that are equipped with, is also that inconvenient tester uses.Therefore be sought after a kind of case, reagent bottle and all auxiliary detection articles for use collection can be contained in a box and take as a unit, only need get a box when tester uses and get final product implementation and operation, use and can deposit back former box censorship, this will be very convenient act, so also can avoid causing secondary pollution simultaneously.
The utility model content
The utility model provides a kind of kit of the ELISA for detection of porcine reproductive and respiratory syndrome antibody, only need get a box during use and get final product implementation and operation.
The utility model provides a kind of ELISA kit that detects porcine reproductive and respiratory syndrome antibody, comprise box body and the spiral-lock lid on box body, described box body middle part is provided with the cavity that is suitable for putting ice bag, described cavity left side is provided with 7 cylindrical grooves, 7 bottles of reagent be positioned over respectively with 7 adaptive cylindrical grooves of its size in, described cavity right side is provided with a rectangular slot, and 96 hole ELISA Plate are placed therein, and adaptive with its size; Described 7 bottles of reagent are respectively cleansing solution, serum dilution, chromogenic substrate, the anti-pig ELIAS secondary antibody of rabbit, stop buffer, positive serum, negative serum; Described 96 hole ELISA Plate are coated with the restructuring GP5 proteantigen of porcine reproductive and respiratory syndrome virus GP5 protein gene, and seal with bovine serum albumin(BSA).
Preferably, the material of described kit is polystyrene foam.
Preferably, described 96 hole ELISA Plate have the Fresco Bag external packing, in described Fresco Bag external packing inside, also are provided with desiccant bag.
Preferably, described serum dilution is loaded on the clear PE plastic bottle of green cap, described chromogenic substrate is loaded on the clear PE plastic bottle of red cap, the anti-pig ELIAS secondary antibody of described rabbit is loaded on the clear PE plastic bottle of white cap, described stop buffer is loaded on the clear PE plastic bottle of yellow cap, described cleansing solution is loaded on the clear PE plastic bottle of black caps, and described positive serum is loaded on the clear PE plastic bottle of green cap, and described negative serum is loaded on the clear PE plastic bottle of red cap.
Preferably, be respectively equipped with around described box body surrounding the cavity that is suitable for placing ice bag.
Preferably, described cleansing solution is 200ml, and described serum dilution is 100ml, and described chromogenic substrate is 100ml, and the anti-pig ELIAS secondary antibody of described rabbit is 100ml, and described stop buffer is 100ml, and described positive serum is 5ml, and described negative serum is 5ml.
When kit of the present utility model will detect porcine reproductive and respiratory syndrome antibody, agents useful for same all is placed in kit, when detecting porcine reproductive and respiratory syndrome antibody, only kit of the present utility model need to be opened, reagent is wherein taken out and can use, clean hygiene, take conveniently, is easy to carry about with one, avoid the mistake of reagent to put, leaked and put, improved operating personnel's work efficiency.
The accompanying drawing explanation
The structural representation that Fig. 1 is the utility model kit;
The another kind of structural representation that Fig. 2 is the utility model kit;
Wherein, 1 is box body, and 2 is lid, and 3 is cavity, and 4 is cylindrical groove, and 5 is rectangular slot.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.
As shown in Figure 1: a kind of ELISA kit that detects porcine reproductive and respiratory syndrome antibody of the present utility model, comprise box body 1 and the spiral-lock lid 2 on box body, box body 1 middle part is provided with the cavity 3 that is suitable for putting ice bag, cavity 3 left sides are provided with 7 cylindrical grooves 4,7 bottles of reagent be positioned over respectively with 7 adaptive cylindrical grooves 4 of its size in, cavity 3 right sides are provided with a rectangular slot 5,96 hole ELISA Plate and are placed therein, and adaptive with its size; 7 bottles of reagent are respectively cleansing solution, serum dilution, chromogenic substrate, the anti-pig ELIAS secondary antibody of rabbit, stop buffer, positive serum, negative serum; 96 hole ELISA Plate are coated with the restructuring GP5 proteantigen of porcine reproductive and respiratory syndrome virus GP5 protein gene, and seal with bovine serum albumin(BSA).
The material of kit is polystyrene foam preferably, and polystyrene foam has the effect of well heat insulation and damping.
In order to keep the stable of antigen, 96 hole ELISA Plate have the Fresco Bag external packing, in Fresco Bag external packing inside, also are provided with desiccant bag.
For the ease of distinguishing each reagent, serum dilution is loaded on the clear PE plastic bottle of green cap, chromogenic substrate is loaded on the clear PE plastic bottle of red cap, the anti-pig ELIAS secondary antibody of rabbit is loaded on the clear PE plastic bottle of white cap, stop buffer is loaded on the clear PE plastic bottle of yellow cap, cleansing solution is loaded on the clear PE plastic bottle of black caps, and positive serum is loaded on the clear PE plastic bottle of green cap, and negative serum is loaded on the clear PE plastic bottle of red cap.
In order to reach better effect, be respectively equipped with around described box body surrounding the cavity that is suitable for placing ice bag, ice bag is placed therein.
In kit of the present utility model, the volume of every reagent is respectively: cleansing solution is 200ml, and serum dilution is 100ml, and chromogenic substrate is 100ml, and the anti-pig ELIAS secondary antibody of rabbit is 100ml, and stop buffer is 100ml, and positive serum is 5ml, and negative serum is 5ml.
Also be placed with instructions in kit.During use, reagent in kit is taken out, the operation steps in to specifications operates and gets final product.Various reagent by color different caps distinguish, easy to use.After finishing using, put back to kit and preserve.
Finally it should be noted that: the foregoing is only preferred embodiment of the present utility model, be not limited to the utility model, although with reference to previous embodiment, the utility model is had been described in detail, for a person skilled in the art, its technical scheme that still can put down in writing aforementioned each embodiment is modified, or part technical characterictic wherein is equal to replacement.All within spirit of the present utility model and principle, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection domain of the present utility model.
Claims (6)
1. an ELISA kit that detects porcine reproductive and respiratory syndrome antibody, comprise box body and the spiral-lock lid on box body, it is characterized in that: described box body middle part is provided with the cavity that is suitable for putting ice bag, described cavity left side is provided with 7 cylindrical grooves, 7 bottles of reagent be positioned over respectively with 7 adaptive cylindrical grooves of its size in, described cavity right side is provided with a rectangular slot, and 96 hole ELISA Plate are placed therein, and adaptive with its size; Described 7 bottles of reagent are respectively cleansing solution, serum dilution, chromogenic substrate, the anti-pig ELIAS secondary antibody of rabbit, stop buffer, positive serum, negative serum; Described 96 hole ELISA Plate are coated with the restructuring GP5 proteantigen of porcine reproductive and respiratory syndrome virus GP5 protein gene, and seal with bovine serum albumin(BSA).
2. kit according to claim 1, it is characterized in that: the material of described kit is polystyrene foam.
3. kit according to claim 1, it is characterized in that: described 96 hole ELISA Plate have the Fresco Bag external packing, in described Fresco Bag external packing inside, also are provided with desiccant bag.
4. kit according to claim 1, it is characterized in that: described serum dilution is loaded on the clear PE plastic bottle of green cap, described chromogenic substrate is loaded on the clear PE plastic bottle of red cap, the anti-pig ELIAS secondary antibody of described rabbit is loaded on the clear PE plastic bottle of white cap, described stop buffer is loaded on the clear PE plastic bottle of yellow cap, described cleansing solution is loaded on the clear PE plastic bottle of black caps, described positive serum is loaded on the clear PE plastic bottle of green cap, and described negative serum is loaded on the clear PE plastic bottle of red cap.
5. kit according to claim 1, is characterized in that: around described box body surrounding, be respectively equipped with the cavity that is suitable for placing ice bag.
6. kit according to claim 1, it is characterized in that: described cleansing solution is 200ml, described serum dilution is 100ml, described chromogenic substrate is 100ml, the anti-pig ELIAS secondary antibody of described rabbit is 100ml, described stop buffer is 100ml, and described positive serum is 5ml, and described negative serum is 5ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013203794954U CN203324277U (en) | 2013-06-28 | 2013-06-28 | ELISA kit used for detecting porcine reproductive and respiratory syndrome antibodies |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013203794954U CN203324277U (en) | 2013-06-28 | 2013-06-28 | ELISA kit used for detecting porcine reproductive and respiratory syndrome antibodies |
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| CN203324277U true CN203324277U (en) | 2013-12-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2013203794954U Expired - Fee Related CN203324277U (en) | 2013-06-28 | 2013-06-28 | ELISA kit used for detecting porcine reproductive and respiratory syndrome antibodies |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103336114A (en) * | 2013-06-28 | 2013-10-02 | 无锡同心塑料制品有限公司 | ELISA (Enzyme Linked Immuno-Sorbent Assay) kit for detecting porcine reproductive and respiratory syndrome |
| CN103698514A (en) * | 2013-12-17 | 2014-04-02 | 广西大学 | ELISA (Enzyme Linked Immunosorbent Assay) kit for detecting lawsonia intracellularis antibody |
-
2013
- 2013-06-28 CN CN2013203794954U patent/CN203324277U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103336114A (en) * | 2013-06-28 | 2013-10-02 | 无锡同心塑料制品有限公司 | ELISA (Enzyme Linked Immuno-Sorbent Assay) kit for detecting porcine reproductive and respiratory syndrome |
| CN103698514A (en) * | 2013-12-17 | 2014-04-02 | 广西大学 | ELISA (Enzyme Linked Immunosorbent Assay) kit for detecting lawsonia intracellularis antibody |
| CN103698514B (en) * | 2013-12-17 | 2015-11-18 | 广西大学 | Detect the ELISA kit of pig Lawsonia intracellularis antibody |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131204 Termination date: 20140628 |
|
| EXPY | Termination of patent right or utility model |