CN206696296U - A kind of Western blotting film - Google Patents
A kind of Western blotting film Download PDFInfo
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- CN206696296U CN206696296U CN201720304574.7U CN201720304574U CN206696296U CN 206696296 U CN206696296 U CN 206696296U CN 201720304574 U CN201720304574 U CN 201720304574U CN 206696296 U CN206696296 U CN 206696296U
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- film
- western blotting
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- 238000001262 western blot Methods 0.000 title claims abstract description 63
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 44
- 239000000758 substrate Substances 0.000 claims description 20
- 239000011248 coating agent Substances 0.000 claims description 9
- 238000000576 coating method Methods 0.000 claims description 9
- 239000004677 Nylon Substances 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 8
- 229920001778 nylon Polymers 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims 1
- 229920002678 cellulose Polymers 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims 1
- 239000000427 antigen Substances 0.000 abstract description 18
- 102000036639 antigens Human genes 0.000 abstract description 18
- 108091007433 antigens Proteins 0.000 abstract description 18
- 238000001514 detection method Methods 0.000 abstract description 8
- 238000012360 testing method Methods 0.000 abstract description 8
- 238000006243 chemical reaction Methods 0.000 abstract description 6
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 21
- 238000004519 manufacturing process Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 239000000020 Nitrocellulose Substances 0.000 description 6
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical group O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 6
- 239000007853 buffer solution Substances 0.000 description 6
- 229920001220 nitrocellulos Polymers 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 239000013641 positive control Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 239000013642 negative control Substances 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 2
- 239000004205 dimethyl polysiloxane Substances 0.000 description 2
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 2
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 2
- 229920005573 silicon-containing polymer Polymers 0.000 description 2
- 238000006884 silylation reaction Methods 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 239000002033 PVDF binder Substances 0.000 description 1
- BOTDANWDWHJENH-UHFFFAOYSA-N Tetraethyl orthosilicate Chemical compound CCO[Si](OCC)(OCC)OCC BOTDANWDWHJENH-UHFFFAOYSA-N 0.000 description 1
- CHBCHAGCVIMDKI-UHFFFAOYSA-N [F].C=C Chemical compound [F].C=C CHBCHAGCVIMDKI-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000002038 chemiluminescence detection Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- JZZIHCLFHIXETF-UHFFFAOYSA-N dimethylsilicon Chemical compound C[Si]C JZZIHCLFHIXETF-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229920001600 hydrophobic polymer Polymers 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- -1 oxygen alkane Chemical class 0.000 description 1
- 238000010422 painting Methods 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 238000012764 semi-quantitative analysis Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 125000004469 siloxy group Chemical group [SiH3]O* 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- YUYCVXFAYWRXLS-UHFFFAOYSA-N trimethoxysilane Chemical compound CO[SiH](OC)OC YUYCVXFAYWRXLS-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Abstract
The utility model discloses a kind of Western blotting film.The Western blotting film surface has with multiple parallel strip hydrophilic regions at hydrophobic region interval, and the strip hydrophilic region has one or more lands, has target antigen on the land, for combining the target antibody of Western blotting.On Western blotting film of the present utility model, the albumen sample of multiple target antibodies can be detected simultaneously, so as to improve the reaction efficiency of Parallel testing.Meanwhile Western blotting film has with multiple parallel strip hydrophilic regions at hydrophobic region interval, is combined by the absorption affinity of hydrophilic region with target antibody, so as to more save reagent, while it is applied to trace detection.
Description
Technical field
The utility model belongs to protein analysis field, more particularly, to a kind of Western blotting film.
Background technology
Western blotting (Western Blot) technology is by Protein transfer to film, is then detected using antibody
Technology.Western blotting is usually used in identifying certain albumen, and can carry out quantitative and semi-quantitative analysis to albumen.Examined with reference to chemiluminescence
Survey, can simultaneously more multiple sample same proteins expression difference.Because Western blotting has SDS-PAGE high-resolution
The high specific and sensitiveness of power and solid-phase immunoassay, as a kind of routine techniques of analysis of protein.
Film used in immunoblot assay is nitrocellulose filter, nylon membrane or polyvinylidene fluoride film, is shaped as strip
(《Antibody technique experiment guide》, Science Press, 2002.9).The film is only capable of carrying out the single detection of single sample to be tested,
If the Parallel testing of multiple samples to be tested need to be carried out, need after the plurality of albumen sample is transferred on different films, for
Different films are individually incubated, while are vibrated to accelerate reaction speed.Therefore had the disadvantages that using the film：
First, when carrying out Parallel testing, reaction efficiency is low；2nd, because Western blotting film needs to be immersed in sample to be tested solution,
Required sample size is big, expends reagent, and be difficult to trace detection；3rd, film needs to develop the color after being dried and taken pictures, then enters
Row analysis, so as to which in situ detection can not be realized；4th, because film is generally immersed in different windows or reactive tank, different films
Reaction condition slightly have difference, so as to influence the accuracy of Western blotting.Disadvantage mentioned above also result in enter using the film indirectly
The structure of the automatic detection instrument of row Western blotting is complex, and production cost is higher.
Utility model content
For the disadvantages described above or Improvement requirement of prior art, the utility model provide a kind of Western blotting film and its
Preparation method, its object is to which film surface is divided into multiple hydrophilic regions, so as to realize the parallel inspection of multiple albumen samples
Survey.
To achieve the above object, according to one side of the present utility model, there is provided a kind of Western blotting film, it is described to exempt from
Epidemic disease trace film surface has and had with multiple parallel strip hydrophilic regions at hydrophobic region interval, the strip hydrophilic region
One or more lands, there is target antigen on the land, for combining the target antibody of Western blotting.
Preferably, the strip hydrophilic region has multiple lands, and the multiple land includes test block and right
According to area.
As it is further preferred that the check plot includes positive control area, negative control area or conjugate check plot.
Preferably, the material of the strip hydrophilic region is nitrocellulose, nylon or Kynoar.
Preferably, the hydrophobic region has silylation.
As it is further preferred that the material of the hydrophobic region is dimethyl silicone polymer or derivatives thereof.
Preferably, the width of the strip hydrophilic region and hydrophobic region is 1mm~5mm.
Preferably, direction of the Western blotting film along strip hydrophilic region is around being made as spool structure.
As it is further preferred that a diameter of 2cm~6cm of the spool structure.
As it is further preferred that the spool structure is wound in support shaft.
As it is further preferred that the support shaft is cylindric or cylindric.
Preferably, the Western blotting film (1) includes substrate film and is covered in the hydrophobic painting on the substrate film surface
Material, the hydrophobic coating forms hydrophobic region (11), and substrate film is divided into multiple strip hydrophilic regions (12).
Preferably, the Western blotting film includes hydrophobic film and is covered in multiple strips parent on hydrophobic film surface
Water film, the multiple strip hydrophilic film be arranged in parallel, form multiple parallel strip hydrophilic regions.
It is described as it is further preferred that the hydrophobic film surface has the groove that is engaged with strip hydrophilic film
Strip hydrophilic film is fixed in the groove.
According to another aspect of the present utility model, a kind of preparation method of above-mentioned Western blotting film is additionally provided, including：
The combining target antigen on substrate film, to form the quantity identical junction belt with target antigen, the substrate film
Material be nitrocellulose, nylon or Kynoar；
Hydrophobic region is formed on substrate film with hydrophobic coating, substrate film is divided into multiple strip hydrophilic regions, together
When junction belt is divided into land so that each strip hydrophilic region has to be combined with the quantity identical of target antigen
Area.
According to another aspect of the present utility model, a kind of preparation method of above-mentioned Western blotting film is additionally provided, including：
The parallel patch of multiple strip hydrophilic films is formed on hydrophobic film so that have and multiple on the hydrophobic film
Shape hydrophilic film formed multiple parallel strip hydrophilic regions, the strip hydrophilic region with hydrophobic region separately.
Preferably, the hydrophobic film surface has the groove being engaged with strip hydrophilic film, and the groove is used for solid
Determine strip hydrophilic film.
In general, by the contemplated above technical scheme of the utility model compared with prior art, due to by dredging
Aqua region, Western blotting film is divided into multiple parallel strip hydrophilic regions, following beneficial effect can be obtained：
1st, on same Western blotting film, the sample of multiple target antibodies can be detected, so as to improve parallel inspection
The reaction efficiency of survey；
2nd, Western blotting film has with multiple parallel strip hydrophilic regions at hydrophobic region interval, passes through hydrophilic region
Absorption affinity combined with target antibody, so as to more saving reagent, while be applied to trace detection；
3rd, Western blotting film by the absorption affinity of hydrophilic region with target antibody due to being combined, so that required is molten
Liquid measure is less, simplifies detecting step, is more suitable in situ detection；
4th, the sample of multiple target antibodies is incorporated on same Western blotting film, so as to be more suitable for identical
Under the conditions of reacted, it is easier to carry out parallel control, improve the accuracy rate of detection.
Brief description of the drawings
Fig. 1 is the top view of the utility model embodiment 1；
Fig. 2 a are the manufacturing process schematic diagram of the utility model embodiment 1；
Fig. 2 b are the manufacturing process schematic diagram of the utility model embodiment 1；
Fig. 2 c are the manufacturing process schematic diagram of the utility model embodiment 1；
Fig. 3 is the manufacturing process schematic diagram of the utility model embodiment 2；
Fig. 4 is the manufacturing process schematic diagram of the utility model embodiment 3；
Fig. 5 is the structural representation of the utility model embodiment 4；
In all of the figs, identical reference is used for representing identical element or structure, wherein：1- Western blottings
Film, 11- hydrophobic regions, 12- hydrophilic regions, 13- lands, 2- support shafts.
Embodiment
In order that the purpose of this utility model, technical scheme and advantage are more clearly understood, below in conjunction with accompanying drawing and implementation
Example, the utility model is further elaborated.It should be appreciated that specific embodiment described herein is only explaining
The utility model, it is not used to limit the utility model.In addition, institute in each embodiment of the utility model disclosed below
As long as the technical characteristic being related to does not form conflict each other, can is mutually combined.
The utility model provides a kind of Western blotting film 1, and the surface of Western blotting film 1 has with hydrophobic region
Multiple strip hydrophilic regions 12 at 11 intervals, the material of the strip hydrophilic region 12 are nitrocellulose, nylon or gather inclined fluorine
Ethene, so as to combining target antigen, the hydrophobic region 11 is usually organic hydrophobic polymer with silylation, also simultaneously
Siloxy or silicone hydroxyl may be carried, such as dimethyl silicone polymer or derivatives thereof；
The strip hydrophilic region 12 has one or more lands 13 along its length, has on the land 13
There is target antigen, the target antigen is used to be combined with the target protein of Western blotting；When the strip hydrophilic region 12 includes
During multiple lands 13, land 13 can be divided into test block and check plot, and test block is used to be combined with target protein, and compares
Area can be divided into positive control area, negative control area or conjugate check plot according to its function；Wherein, positive control area generally contains
There is the antigen that can be combined with other compositions in solution to be measured in addition to target antibody, for example, when using blood as solution to be measured,
The target antigen of positive control area can then use serum antibody, and positive control area colour developing proves that Western blotting film 1 does not go bad, and
Solution type to be measured is errorless；And negative control area can be coated with unlabelled secondary antibody so that negative control area at the standard conditions
It can not develop the color, if it develops the color, it was demonstrated that the abnormal conditions such as may have Western blotting film 1 rotten, it should change Western blotting film 1
And redeterminate；And the target antigen of conjugate check plot is IgG conjugates, IgM conjugates or IgA conjugates, due to IgG,
IgM and IgA is common immunoglobulin, and the presence of conjugate check plot may indicate that whether target antibody has successfully combined.
The width of the strip hydrophilic region 12 and hydrophobic region 11 is usually 1mm~5mm, is being easy to do Western blotting
While test, it is ensured that each strip hydrophilic region 12 is spaced completely, while effectively utilizes the space of Western blotting film 1.
For the ease of storing and using, the Western blotting film 1 can be along the direction of strip hydrophilic region around cylindric
Or cylindric support shaft 2 is around the spool structure for being made as a diameter of 2cm~6cm, as shown in Figure 5.
The preparation method of the Western blotting film 1 is roughly divided into two kinds：
The first：The combining target antigen on substrate film, it is described to form the quantity identical junction belt with target antigen
The material of substrate film is nitrocellulose, nylon or Kynoar；Hydrophobic region 11 is formed on substrate film with hydrophobic coating,
So that substrate film is divided into multiple strip hydrophilic regions 12, while by junction belt land 13 corresponding with junction belt so that every
Individual strip hydrophilic region 12 all has the quantity identical land 13 with target antigen；Hydrophobic coating needs are nontoxic, and
Less than 37 degree can dry, in order to avoid the activity of target antigen is influenceed, such as the hydrophobic coating in patent document CN201380057250.
Second：The parallel patch of multiple strip hydrophilic films is formed on hydrophobic film so that have on the hydrophobic film
With multiple strip hydrophilic films formed strip hydrophilic region 12, the strip hydrophilic region 12 with hydrophobic region 11 separately；
The hydrophobic film surface can be provided with the groove being engaged with strip hydrophilic film, to be combined simultaneously in strip hydrophilic film
It is fixed so that the surface where strip hydrophilic region 12 is less than the surface where hydrophobic region, can so be further ensured that strip
Reaction solution between hydrophilic region 12 will not interact mixing.
Above-mentioned Western blotting film 1 can be applied to immune-blotting method, so that indirect method carries out chemiluminescence detection as an example, tool
Body comprises the following steps：
S1. set above away from strip hydrophilic region 12 at 0.1mm~2mm corresponding with the quantity of strip hydrophilic region 12
Liquid-feeding tube, a variety of solution to be measured are made in solution to be measured by liquid-feeding tube with making an addition to the different surfaces of strip hydrophilic region 12
Target antibody and junction belt on target antigen be completely combined；Simultaneously so that the strip hydrophilic region 12 of liquid-feeding tube is with 1cm/s
~15cm/s speed does relatively reciprocating motion, and while motion, still can be slowly added solution to be measured in bar by liquid-feeding tube
The surface of shape hydrophilic region 12, to prevent liquid evaporation, to keep the liquid level on strip hydrophilic region 12 as the mm of 0.1mm~1；
In the case, it is computed, even if the solution to be measured remained in liquid-feeding tube is counted, the volume of required solution to be measured
Several tens of microliters is only needed to milliliter level；
S2. collector tube can be set in one end of each strip hydrophilic region 12, one end of collector tube is away from strip hydrophilic region 12
Top 0.1mm~2mm, other end connection vavuum pump, after completion of the reaction, vavuum pump aspirates strip hydrophilic region by collector tube
Unnecessary solution to be measured on 12；
S3. in a manner of same in S1-S2., the solution to be measured in former liquid-feeding tube is substituted with buffer solution, Western blotting is thin
The solution to be measured on the surface of film 1 is rinsed well；Because buffer solution is usually the lower-cost solution such as PBS, relative to solution to be measured,
More volume can be added；
S4. in a manner of same in S1-S2., the buffer solution in former liquid-feeding tube is substituted with two corresponding anti-solution so that secondary antibody is complete
Combined with target antibody；
S5. in a manner of same in S3., the two corresponding anti-solution in former liquid-feeding tube is substituted with buffer solution, by Western blotting film 1
The two corresponding anti-solution on surface is rinsed well；
S6. in a manner of same in S1-S2., the buffer solution in former liquid-feeding tube is substituted with chemiluminescence agent solution so that mark
The secondary antibody recorded a demerit lights；
S7. in a manner of same in S3., the chemiluminescence agent solution in former liquid-feeding tube is substituted with buffer solution, by Diagnosis of Sghistosomiasis
The chemiluminescence agent solution on the surface of mark film 1 is rinsed well；
S8. adaptive immune trace testing result.
Embodiment 1
Fig. 1 is the Western blotting film 1 of the utility model embodiment 1, and its material is nylon membrane；The Western blotting film 1
Surface has with multiple strip hydrophilic regions 12 at the interval of hydrophobic region 11, the strip hydrophilic region 12 and hydrophobic region 11
Width be 1mm, the strip hydrophilic region 12 has one or more lands 13, has mesh on the land 13
Mark antigen；
The preparation method of the Western blotting film 1 first on the substrate film of nylon with reference to four kinds of targets as shown in Fig. 2 resist
Original, to form four junction belts, as shown in Fig. 2 a-b；Then hydrophobic region 11 is formed on substrate film with hydrophobic coating, will
Substrate film is divided into multiple strip hydrophilic regions 12, at the same by junction belt be divided into junction belt quantity identical land 13,
So that each strip hydrophilic region 12 has the quantity identical land 13 with target antigen, as shown in Figure 2 c, that is, obtain
Western blotting film 1 shown in Fig. 1.The composition of hydrophobic coating be 1.448wt% tetraethyl orthosilicate, the ten of 0.1588wt%
Dialkyl group trimethoxy silane, 0.0698wt% ammonium hydroxide, 0.1938wt% HCl, 208wt% ethanol and
78.1198wt% softened water.
Embodiment 2
Fig. 3 is the manufacturing process schematic diagram of the Western blotting film 1 of the utility model embodiment 2, first places poly dimethyl silicon
The substrate of oxygen alkane (PDMS), will be then 3mm cellulose nitrate film between 3mm with multiple lands 13, width
Every being sticked with substrate.
Embodiment 3
Fig. 4 is the manufacturing process schematic diagram of the Western blotting film 1 of the utility model embodiment 3, and the difference with embodiment 2 exists
In on the PDMS substrates, at interval of 5mm, there is the wide grooves of a 5mm, width is that 5mm polyvinylidene difluoride film can be firm
It is sticked with well in the groove.
Embodiment 4
Fig. 5 is the structural representation of 4 Western blotting film of the utility model embodiment 1, and the difference with embodiment 1 is, long
The Western blotting film 1 spent for 10cm is wound in the support shaft 2 that diameter is about 3.2cm.
As it will be easily appreciated by one skilled in the art that preferred embodiment of the present utility model is the foregoing is only, not
To limit the utility model, any modification of all made within spirit of the present utility model and principle, equivalent substitution and change
Enter, should be included within the scope of protection of the utility model.
Claims (9)
1. a kind of Western blotting film (1), it is characterised in that Western blotting film (1) surface has with hydrophobic region
(11) multiple parallel strip hydrophilic regions (12) at interval, the strip hydrophilic region (12) have one or more lands
(13)。
2. Western blotting film (1) as claimed in claim 1, it is characterised in that the material of the strip hydrophilic region is nitre
Acid cellulose, nylon or Kynoar.
3. Western blotting film (1) as claimed in claim 1, it is characterised in that the strip hydrophilic region (12) and dredge
The width of aqua region (11) is 1mm~5mm.
4. the Western blotting film (1) as described in any one in claim 1-3, it is characterised in that the Western blotting is thin
Direction of the film (1) along strip hydrophilic region (12) is around being made as spool structure.
5. Western blotting film (1) as claimed in claim 4, it is characterised in that the spool structure is wound in support shaft (2)
On.
6. Western blotting film (1) as claimed in claim 4, it is characterised in that a diameter of 2cm of the spool structure~
6cm。
7. Western blotting film (1) as claimed in claim 1, it is characterised in that the Western blotting film (1) includes substrate
Film and the hydrophobic coating for being covered in the substrate film surface, the hydrophobic coating form hydrophobic region (11), and by substrate film
It is divided into multiple strip hydrophilic regions (12).
8. Western blotting film (1) as claimed in claim 1, it is characterised in that the Western blotting film (1) includes hydrophobic
Film and the multiple strip hydrophilic films for being covered in hydrophobic film surface, the multiple strip hydrophilic film be arranged in parallel, shape
Into multiple parallel strip hydrophilic regions (12).
9. Western blotting film (1) as claimed in claim 8, it is characterised in that the hydrophobic film surface has and strip
The groove that hydrophilic film is engaged, the strip hydrophilic film are fixed in the groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720304574.7U CN206696296U (en) | 2017-03-27 | 2017-03-27 | A kind of Western blotting film |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720304574.7U CN206696296U (en) | 2017-03-27 | 2017-03-27 | A kind of Western blotting film |
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| Publication Number | Publication Date |
|---|---|
| CN206696296U true CN206696296U (en) | 2017-12-01 |
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| CN201720304574.7U Expired - Fee Related CN206696296U (en) | 2017-03-27 | 2017-03-27 | A kind of Western blotting film |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106932596A (en) * | 2017-03-27 | 2017-07-07 | 武汉优视科技有限公司 | A kind of Western blotting film and preparation method thereof |
| CN110975952A (en) * | 2019-12-10 | 2020-04-10 | 华中科技大学 | Paper-based microfluid chip and preparation method and application thereof |
-
2017
- 2017-03-27 CN CN201720304574.7U patent/CN206696296U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106932596A (en) * | 2017-03-27 | 2017-07-07 | 武汉优视科技有限公司 | A kind of Western blotting film and preparation method thereof |
| CN110975952A (en) * | 2019-12-10 | 2020-04-10 | 华中科技大学 | Paper-based microfluid chip and preparation method and application thereof |
| CN110975952B (en) * | 2019-12-10 | 2020-11-17 | 华中科技大学 | Paper-based microfluid chip and preparation method and application thereof |
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