CN206680459U - Novel microbial solid medium card punch - Google Patents

Novel microbial solid medium card punch Download PDF

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Publication number
CN206680459U
CN206680459U CN201720281801.9U CN201720281801U CN206680459U CN 206680459 U CN206680459 U CN 206680459U CN 201720281801 U CN201720281801 U CN 201720281801U CN 206680459 U CN206680459 U CN 206680459U
Authority
CN
China
Prior art keywords
punching ring
card punch
punching
ring
culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201720281801.9U
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Chinese (zh)
Inventor
张溢峰
田迪
白先放
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangxi University
Original Assignee
Guangxi University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangxi University filed Critical Guangxi University
Priority to CN201720281801.9U priority Critical patent/CN206680459U/en
Application granted granted Critical
Publication of CN206680459U publication Critical patent/CN206680459U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

This novel microbial solid medium card punch, punching ring therein is the hollow cylinder of tubular, punching ring is angled 100 ° 150 ° with punching ring rod, when card punch is tiltedly put in insertion culture dish, it is vertical with the solid culture primary surface in culture dish to punch ring wall, regular cylindrical body shape solid culture based specimen can be got when pushing card punch, there is 10 40 micro-holes, a diameter of 0.11cm 0.30cm of micro-hole on punching ring.The utility model novel microbial solid medium card punch can get regular cylindrical body shape solid culture based specimen, avoid culture dish opening excessive, the youngster for reducing living contaminants leads, time required for reducing during heat sterilization, after solving solid medium punching, culture medium is sticked be not easy to fall on inwall the problem of, substantially increases conventional efficient.

Description

Novel microbial solid medium card punch
【Technical field】
It the utility model is related to a kind of card punch, particularly microbiological culture media card punch
【Background technology】
Card punch, it is widely used in medical science, agronomy, industry etc. microculture and face-off experiment, in microorganism In related science research, it is related to solid medium card punch, is widely used in the cutting of solid medium.At present, commonly use solid Body culture medium cutting method is then using the "T"-shaped card punch cutting punched to solids.The patent No. Large area sterilizing dead angle occurs in CN201320592980.X, Experiment on Microbiology card punch, it is impossible to complete sterilizing, and also it is this Setting type card punch, need to open culture dish lid completely in punching, bacterial spore can disorderly fly everywhere, asking for living contaminants occur Topic, increase pollution probability.Patent No. CN201420388064.9 is improved, and in drill process, is cultivated without opening completely It ware lid, only need to open a seam, in the laterally inserted culture dish of card punch, carry out punching sampling.When the horizontally-parallel insertion of card punch When in culture dish, because culture dish wall is highly more than punching ring height, card punch can not touch the solid culture in culture dish Base, it can not sample, in order to carry out punching sampling, it is therefore desirable to tiltedly put card punch, oblique hole can only be got by tiltedly putting card punch, punching The culture medium solid block of sampling out is irregular, is unfavorable for testing, and when carrying out heat sterilization to card punch, firing rate Slowly, efficiency is low.
【The content of the invention】
To solve problem above, the utility model provides a kind of novel microbial solid medium card punch, can solved miscellaneous Bacterium pollution problem, make experimental result with more science.
The utility model novel microbial solid medium card punch, there is punching ring, punching ring bar, connecting rod and thermal insulation Handle, it is characterised in that:Punching ring is the hollow cylinder of tubular, and phase is welded at punching ring bar and the one of punching ring wall upper end Even, the punching ring wall on weld vertical direction forms fixed angle with punching ring bar, and its angle is 100 ° -150 °, when beating When hole device is tiltedly put in insertion culture dish, punching ring wall is vertical with the solid culture primary surface in culture dish, can when pushing card punch To get regular cylindrical body shape solid culture based specimen, avoid culture dish opening excessive, reduce the probability of living contaminants;Punching ring Bar and connecting rod are designed as the formula of being weldingly connected, and both are tightly secured together;There are 10-40 micro-hole, micro-hole on punching ring A diameter of 0.11cm-0.30cm, micro-hole is set to increase contact of the solid medium with air after punching in punching ring Face, it is easy to the solid medium in punching ring to depart from perforating head, on the other hand, is also convenient for before experiment starts or is fought each other after terminating Orifice ring carries out heat sterilization, is that sterilizing efficiency is higher.
The utility model novel microbial solid medium card punch, it is characterised in that punching ring is hollow cylinder Structure, the internal diameter of punching ring is 0.2cm-1.5cm, is highly 0.3cm-1.0cm, and the length of connecting rod is 3.5cm-6.0cm, hand Handle outer wrapping layer of insulation material.
The utility model novel microbial solid medium card punch can get regular cylindrical body shape solid culture basic pattern Product, avoid culture dish opening excessive, reduce the probability of living contaminants, the time required for reducing during heat sterilization, solution After solid medium of having determined punching, culture medium is sticked be not easy to fall on inwall the problem of, substantially increases conventional efficient.
【Brief description of the drawings】
Fig. 1 is structural representation of the present utility model;Fig. 2 is the utility model punching ring structural representation
1st, punching ring;2nd, micro-hole;3rd, sword is punched;4th, punching ring bar;5th, connecting rod;6th, heat insulation bat-handle
【Embodiment】
A kind of novel microbial solid medium card punch, including punching ring bar (4), punching ring (1), punching sword (3), company Extension bar (5), insulated handle (6).Punching ring bar (4) length 5cm, one end are closely weldingly connected with punching ring (1), punching ring bar (4) it is connected with punching ring (1) and forms 120 ° of angle;The other end of punching ring bar (4) closely welds phase with connecting rod (5) Even, the other end and heat insulation bat-handle (6) of connecting rod (5) are closely coupled.Punching ring (1) is tube-shaped column body, hollow, and outer rim is set It is 0.65cm to be equipped with flat punching sword (3) internal diameter, is highly 0.45cm.Connecting rod (5) closely connects in left end and punching ring bar (4) It is connected to together, right-hand member is fixed together with heat insulation bat-handle (6).

Claims (2)

1. a kind of novel microbial solid medium card punch, there are punching ring, punching ring bar, connecting rod and heat insulation bat-handle, its It is characterised by that punching ring for the hollow cylinder of tubular, is weldingly connected at punching ring bar and the one of punching ring wall upper end, weld Punching ring wall on vertical direction forms fixed angle with punching ring bar, and its angle is 100 ° -150 °, when card punch tiltedly put it is slotting When entering in culture dish, punching ring wall is vertical with the solid culture primary surface in culture dish, and rule can be got when pushing card punch Cylindrical shape solid culture based specimen;Punching ring bar and connecting rod are designed as the formula of being weldingly connected, and both are tightly secured together;Beat There are 10-40 micro-hole, a diameter of 0.11cm-0.30cm of micro-hole on orifice ring.
2. novel microbial solid medium card punch according to claim 1, it is characterised in that punching ring is hollow Cylindrical structure, the internal diameter of punching ring is 0.2cm-1.5cm, is highly 0.3cm-1.0cm, and the length of connecting rod is 3.5cm- 6.0cm, handle outer wrapping layer of insulation material.
CN201720281801.9U 2017-03-22 2017-03-22 Novel microbial solid medium card punch Expired - Fee Related CN206680459U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720281801.9U CN206680459U (en) 2017-03-22 2017-03-22 Novel microbial solid medium card punch

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720281801.9U CN206680459U (en) 2017-03-22 2017-03-22 Novel microbial solid medium card punch

Publications (1)

Publication Number Publication Date
CN206680459U true CN206680459U (en) 2017-11-28

Family

ID=60389907

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201720281801.9U Expired - Fee Related CN206680459U (en) 2017-03-22 2017-03-22 Novel microbial solid medium card punch

Country Status (1)

Country Link
CN (1) CN206680459U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112457997A (en) * 2020-12-18 2021-03-09 福建省烟草公司三明市公司 Trichoderma solid fermentation method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112457997A (en) * 2020-12-18 2021-03-09 福建省烟草公司三明市公司 Trichoderma solid fermentation method
CN112457997B (en) * 2020-12-18 2022-12-09 福建省烟草公司三明市公司 Solid fermentation method for trichoderma

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GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20171128

Termination date: 20190322

CF01 Termination of patent right due to non-payment of annual fee