CN211689059U - A perforating device for inoculating fungus on culture medium - Google Patents

A perforating device for inoculating fungus on culture medium Download PDF

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CN211689059U
CN211689059U CN201922343335.4U CN201922343335U CN211689059U CN 211689059 U CN211689059 U CN 211689059U CN 201922343335 U CN201922343335 U CN 201922343335U CN 211689059 U CN211689059 U CN 211689059U
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head
removable
punching
inoculating
culture medium
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张国财
吕明帅
赵博
岳峥嵘
张博文
马伟虎
杨璟
季竞宇
李一龙
葛玉祥
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Northeast Forestry University
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Northeast Forestry University
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Abstract

本实用新型提供了一种用于培养基上接菌的打孔装置,包括顶盖(1)、手柄(2)、压杆(3)、手柄筒(4)、压杆头(5)和可拆换的打孔头(6)。可拆换的打孔头(6)两端均可连接在手柄筒(4)的底端;压杆(3)位于手柄筒(4)内,且其顶端连接着顶盖(1),压杆(3)下端连接着压杆头并穿过手柄筒(4)的底部以及可到达可拆换的打孔头(6)中。本实用新型的一种用于培养基上接菌的打孔装置,结构简单,使用方便,取样完整,可在打孔时简化操作步骤,提高接菌效率;同时,结合材质特点,可拆换的打孔头(6)是一次性使用的,这样有利于降低接菌时杂菌污染的风险,确保实验操作的准确性。

Figure 201922343335

The utility model provides a punching device for inoculating bacteria on a culture medium, comprising a top cover (1), a handle (2), a pressing rod (3), a handle barrel (4), a pressing rod head (5) and a Removable punch head (6). Both ends of the removable punching head (6) can be connected to the bottom end of the handle barrel (4); the pressing rod (3) is located in the handle barrel (4), and its top end is connected to the top cover (1), and the pressure rod (3) is connected to the top cover (1). The lower end of the rod (3) is connected with the pressing rod head and passes through the bottom of the handle barrel (4) and can reach the removable punching head (6). The perforating device used for inoculating bacteria on the culture medium of the utility model has the advantages of simple structure, convenient use and complete sampling, which can simplify the operation steps during perforation and improve the inoculating efficiency; at the same time, combined with the material characteristics, it can be disassembled and replaced The punching head (6) is disposable, which is beneficial to reduce the risk of contamination by miscellaneous bacteria when inoculating bacteria and ensure the accuracy of experimental operations.

Figure 201922343335

Description

一种用于培养基上接菌的打孔装置A kind of punching device for inoculating bacteria on culture medium

技术领域technical field

本实用新型属于微生物培养实验领域,具体涉及一种可重复利用的打孔器装置。The utility model belongs to the field of microorganism culture experiments, in particular to a reusable hole punch device.

背景技术Background technique

打孔器是一种广泛应用于医学、农学、生物学等微生物培养实验中的工具,目前,在微生物相关科学研究中,有关于在培养皿上接菌的相关试验中,常见做法是用解剖刀的简单切割或者镊子移取将菌接到新的培养皿上,这一过程容易在操作中引起杂菌污染,影响接菌效果,影响实验进展。A hole punch is a tool widely used in microbial culture experiments such as medicine, agronomy, and biology. Simple cutting with a knife or pipetting with tweezers connects the bacteria to a new petri dish. This process is likely to cause bacterial contamination during the operation, affecting the effect of inoculating bacteria and affecting the progress of the experiment.

而较为规范的接菌方式是采用实验室普通的打孔器在培养皿的表面中心位置打取菌饼,再用接菌针将菌饼的反面向上放入要接入的培养皿内。这一过程相对复杂、繁琐并且在其无菌操作的过程中,往往存在灭菌不彻底,存在死角,对实验的成功进行造成不利影响。The more standardized way of inoculating bacteria is to use an ordinary hole punch in the laboratory to punch the bacterial cake at the center of the surface of the petri dish, and then use the inoculating needle to put the reverse side of the bacterial cake into the petri dish to be accessed. This process is relatively complex and cumbersome, and in the process of aseptic operation, there are often incomplete sterilization and dead ends, which adversely affect the success of the experiment.

此外,在移动菌饼和使用接菌针翻转菌饼的过程中,由于菌饼的硬度不同,容易导致菌饼破碎或者不易取出,给微生物实验造成了麻烦。In addition, in the process of moving the bacterial cake and using the inoculating needle to turn the bacterial cake, due to the different hardness of the bacterial cake, it is easy to cause the bacterial cake to be broken or difficult to take out, which causes trouble for the microbiological experiment.

实用新型内容Utility model content

本实用新型所要解决的技术问题是针对现有技术的缺陷,提供一种可重复利用并减少污染,简化操作的适用于在培养皿上进行接菌工作的打孔器。The technical problem to be solved by the utility model is to aim at the defects of the prior art, and to provide a puncher suitable for inoculating bacteria on a petri dish, which can be reused, reduces pollution and simplifies the operation.

一种用于培养基上接菌的打孔装置,包括顶盖(1)、手柄(2)、压杆(3)、手柄筒(4)、压杆头(5)和可拆换的打孔头(6),所述可拆换的打孔头(6)固定连接在所述手柄筒(4)的下端;所述压杆(3)位于所述手柄筒(4)内,且其顶端连接着所述顶盖(1),所述的压杆头(5)位于压杆(3)下端穿过所述手柄筒(4)的底部;与所述的可拆换的打孔头(6)的内部相连接。在所述的培养基上接菌的打孔器上,所述压杆头(5)的底端伸入所述的手柄筒(4)的内部;进一步地,所述的在培养基上接菌的打孔器,手柄筒(4)的底端与可拆换的打孔头(6)连接在一起,且压杆头(5)依旧可伸入可拆换的打孔头(6)内部;在所述的在培养基上接菌的打孔器上,所述顶盖(1)、手柄(2)、压杆(3)、手柄筒(4)、压杆头(5)均采用不锈钢材质。在所述的在培养基上接菌的打孔器上,可拆换的打孔头(6)的部分采用PE材质。A punching device for inoculating bacteria on a culture medium, comprising a top cover (1), a handle (2), a pressing rod (3), a handle barrel (4), a pressing rod head (5) and a removable punch A hole head (6), the removable punching head (6) is fixedly connected to the lower end of the handle barrel (4); the pressure rod (3) is located in the handle barrel (4), and its The top end is connected to the top cover (1), the pressure rod head (5) is located at the lower end of the pressure rod (3) and passes through the bottom of the handle barrel (4); (6) are internally connected. On the puncher for inoculating bacteria on the medium, the bottom end of the pressing rod head (5) extends into the interior of the handle barrel (4); For the hole punch for bacteria, the bottom end of the handle barrel (4) is connected with the removable punch head (6), and the pressure rod head (5) can still extend into the removable punch head (6) Inside; on the described hole puncher for inoculating bacteria on the culture medium, the top cover (1), the handle (2), the pressure rod (3), the handle barrel (4), and the pressure rod head (5) are all Made of stainless steel. On the puncher for inoculating bacteria on the culture medium, the part of the removable punching head (6) is made of PE material.

本实用新型的有益效果为:本实用新型提供的在培养基上接菌的打孔器,使用时食指与中指撑住手柄(2),拇指按住顶盖(1),用可拆换的打孔头(6)的底端对培养皿上菌体进行打孔,一块块孔筒形状的菌块进入到孔筒内,然后按压上端的顶盖(1),通过连接在一起的压杆(3)和压杆头(5);从而有效减少了菌饼的损失,使用方便,取样完整。The beneficial effects of the utility model are as follows: the hole punch provided by the utility model for inoculating bacteria on the culture medium, when in use, the index finger and the middle finger support the handle (2), the thumb presses the top cover (1), and the removable The bottom end of the punching head (6) punches the bacteria on the petri dish, and pieces of bacteria in the shape of a hole cylinder enter the hole cylinder, and then press the top cover (1) at the upper end, and the pressure rods connected together (3) and the pressure rod head (5); thereby effectively reducing the loss of the bacterial cake, easy to use, and complete sampling.

本实用新型的有益效果为:将可拆换的打孔头(6)的顶部拔出,再将原底部安入手柄筒(4)底部,之后直接将可拆换的打孔头(6)内的菌饼推出至新的培养皿内;不使用接菌针;从而有效的节省接菌时间,提高接菌的效率。The beneficial effects of the utility model are as follows: the top of the removable punching head (6) is pulled out, and then the original bottom is placed in the bottom of the handle barrel (4), and then the removable punching head (6) is directly inserted. The bacteria cake inside is pushed out into a new petri dish; the bacteria inoculation needle is not used; thus effectively saving the bacteria inoculation time and improving the bacteria inoculation efficiency.

本实用新型的有益效果为:可拆换的打孔头(6)是PE材质的,打孔头是一次性使用的;从而有效的减少杂菌污染,保证实验进度。The beneficial effects of the utility model are as follows: the removable punching head (6) is made of PE material, and the punching head is disposable; thereby effectively reducing the pollution of miscellaneous bacteria and ensuring the progress of the experiment.

附图说明Description of drawings

图1是本实用新型所述用于培养基上接菌的打孔装置结构示意图。FIG. 1 is a schematic structural diagram of the punching device for inoculating bacteria on a culture medium according to the present invention.

图2是本实用新型所述用于培养基上接菌的打孔装置分离状态示意图。Fig. 2 is a schematic diagram of the separation state of the punching device for inoculating bacteria on a medium according to the present invention.

具体实施方式Detailed ways

下面结合附图对本实用新型作进一步说明:Below in conjunction with accompanying drawing, the utility model is further described:

如图1所示,是一种用于图1是本实用新型所述用于培养基上接菌的打孔装置的结构示意图,特征在于:顶盖(1)、手柄(2)、压杆(3)、手柄筒(4)、压杆头()(5)和可拆换的打孔头(6)。As shown in Figure 1, it is a structural schematic diagram of the punching device used for inoculating bacteria on the culture medium according to Figure 1, characterized in that: a top cover (1), a handle (2), a pressure rod (3), handle barrel (4), pressure rod head () (5) and removable punching head (6).

如图2所示,是一种用于培养基上接菌的打孔装置分离状态示意图,特征在于:可拆换的打孔头(6)是一次性的,同时可拆换的打孔头(6)的两端都是可以与手柄筒(4)的底端相连接的,可以高效的通过压杆(3)与压杆头(5)将菌饼推入新的培养皿内,减少污染。As shown in Figure 2, it is a schematic diagram of the separation state of the punching device used for inoculating bacteria on the culture medium, and is characterized in that: the removable punching head (6) is disposable, and the removable punching head Both ends of (6) can be connected to the bottom end of the handle barrel (4), which can efficiently push the bacteria cake into the new petri dish through the pressing rod (3) and the pressing rod head (5), reducing the Pollution.

实施例1在上述结构中,使用时食指与中指撑住手柄(2),拇指按住顶盖(1),用可拆换的打孔头(6)的底端对培养皿上菌体进行打孔取样,将菌饼存储于可拆换的打孔头(6)内,在可拆换的打孔头(6)与手柄筒(4)地段连接处将可拆换的打孔头(6)拔下,再将原可拆换的打孔头(6)底端作为上端,并与手柄筒(4)底部再次安装在一起,通过连接在一起的压杆(3)和压杆头(5),用拇指推动顶盖(1),通过压杆头(5)下移把可拆换的打孔头(6)内的菌块快速推出,获得完整的菌块,且操作省时省力,提高了工作效率。Embodiment 1 In the above-mentioned structure, the index finger and the middle finger support the handle (2) during use, the thumb presses the top cover (1), and the bottom end of the removable punching head (6) is used to carry out the bacteria on the culture dish. Punch and sample, store the bacteria cake in the removable punching head (6), and place the removable punching head ( 6) Unplug, then use the bottom end of the original removable punching head (6) as the upper end, and install it together with the bottom of the handle barrel (4) again, through the connected pressure rod (3) and the pressure rod head (5), push the top cover (1) with the thumb, and quickly push out the bacterial block in the removable punching head (6) by moving down the pressure rod head (5) to obtain a complete bacterial block, and the operation is time-saving Save labor and improve work efficiency.

在本实施例,可在培养机上接菌的打孔装置,其顶盖(1)、手柄(2)、压杆(3)、手柄筒(4)、压杆头(5)均采用不锈钢材质,可在使用前对打孔器进行消毒、高温、高压灭菌。而可拆换的打孔头(6)是可一次性使用的PE材质,可以有效地降低污染的可能性。In this embodiment, the top cover (1), handle (2), pressing rod (3), handle barrel (4), and pressing rod head (5) of the punching device that can inoculate bacteria on the cultivator are all made of stainless steel. , the punch can be sterilized, high temperature, autoclaved before use. The removable punching head (6) is made of PE material which can be used once, which can effectively reduce the possibility of pollution.

以上显示和描述了本实用新型的基本原理、主要特征和优点。本实用新型还会有各种变化和改进,这些变化和改进都落入要求保护的本实用新型范围内。The basic principles, main features and advantages of the present invention have been shown and described above. The utility model will also have various changes and improvements, which all fall within the scope of the claimed utility model.

Claims (5)

1.一种用于培养基上接菌的打孔装置,其特征在于:包括顶盖(1)、手柄(2)、压杆(3)、手柄筒(4)、压杆头(5)和可拆换的打孔头(6),可拆换的打孔头(6)固定连接在所述手柄筒(4)的下端;压杆(3)位于所述手柄筒(4)内,且其顶端装设有所述顶盖(1),所述的压杆头(5)位于压杆(3)下端穿过所述手柄筒(4)的底部;与所述的可拆换的打孔头(6)相连接,在所述的一种用于培养基上接菌的打孔装置上,所述顶盖(1)的底端伸入所述手柄筒(4)顶端内;进一步地,手柄筒(4)的底端与可拆换的打孔头(6)是连接在一起的且压杆头(5)依旧可伸入可拆换的打孔头(6)中。1. a punching device for inoculating bacteria on a culture medium is characterized in that: comprising a top cover (1), a handle (2), a pressure rod (3), a handle barrel (4), a pressure rod head (5) and a removable punching head (6), the removable punching head (6) is fixedly connected to the lower end of the handle barrel (4); the pressure rod (3) is located in the handle barrel (4), And the top cover (1) is installed at the top, and the pressure rod head (5) is located at the lower end of the pressure rod (3) and passes through the bottom of the handle barrel (4); The punching heads (6) are connected, and on the described punching device for inoculating bacteria on a culture medium, the bottom end of the top cover (1) extends into the top end of the handle barrel (4); Further, the bottom end of the handle barrel (4) is connected with the removable punching head (6), and the pressing rod head (5) can still extend into the removable punching head (6). 2.如权利要求1所述的一种用于培养基上接菌的打孔装置,其特征在于:所述顶盖(1)的底端连接着压杆(3)伸入所述手柄筒(4)内,压杆(3)连接着压杆头(5)可伸入可拆换的打孔头(6)中。2. A kind of punching device for inoculating bacteria on a culture medium as claimed in claim 1, characterized in that: the bottom end of the top cover (1) is connected with a pressure rod (3) and extends into the handle barrel In (4), the pressing rod (3) is connected with the pressing rod head (5) and can extend into the removable punching head (6). 3.如权利要求2所述的一种用于培养基上接菌的打孔装置,其特征在于:可拆换的打孔头(6)两端都可以与手柄筒(4)底端连接,一端打入菌饼,再将可拆换的打孔头(6)转换方向插入手柄筒(4)底端,这个操作可节省使用接菌针的时间,提高接菌效率。3. a kind of punching device for inoculating bacteria on the culture medium as claimed in claim 2 is characterized in that: both ends of the removable punching head (6) can be connected with the bottom end of the handle barrel (4) , one end of the fungus cake is punched, and then the removable punching head (6) is inserted into the bottom end of the handle barrel (4) in a reversed direction. This operation can save the time of using the inoculating needle and improve the inoculating efficiency. 4.如权利要求3所述一种用于培养基上接菌的打孔装置,其特征在于:普通的打孔装置是一体的不可拆换,但该打孔装置的可拆换的打孔头(6)是可以拆分的且是一次性的。4. a kind of punching device for inoculating bacteria on culture medium as claimed in claim 3, is characterized in that: common punching device is integral non-removable, but the removable punching of this punching device The head (6) is detachable and disposable. 5.如权利要求4所述的一种用于培养基上接菌的打孔装置,其特征在于:所述顶盖(1)、手柄(2)、压杆(3)、手柄筒(4)、压杆头(5)均采用不锈钢材质,可拆换的打孔头(6)采用PE材质。5. a kind of punching device for inoculating bacteria on culture medium as claimed in claim 4, is characterized in that: described top cover (1), handle (2), pressing rod (3), handle barrel (4) ) and the pressure rod head (5) are made of stainless steel, and the removable punching head (6) is made of PE material.
CN201922343335.4U 2019-12-24 2019-12-24 A perforating device for inoculating fungus on culture medium Expired - Fee Related CN211689059U (en)

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