CN206616224U - A kind of anaerobic bacteria tests piece - Google Patents
A kind of anaerobic bacteria tests piece Download PDFInfo
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- CN206616224U CN206616224U CN201720297465.7U CN201720297465U CN206616224U CN 206616224 U CN206616224 U CN 206616224U CN 201720297465 U CN201720297465 U CN 201720297465U CN 206616224 U CN206616224 U CN 206616224U
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- anaerobic bacteria
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- adhesive tape
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- 238000012360 testing method Methods 0.000 title claims abstract description 53
- 241001148471 unidentified anaerobic bacterium Species 0.000 title claims abstract description 36
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 65
- 239000001301 oxygen Substances 0.000 claims abstract description 65
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 65
- 239000001963 growth medium Substances 0.000 claims abstract description 31
- 239000002250 absorbent Substances 0.000 claims abstract description 29
- 230000002745 absorbent Effects 0.000 claims abstract description 29
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical group CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 44
- 239000002390 adhesive tape Substances 0.000 claims description 43
- 241000894006 Bacteria Species 0.000 claims description 27
- 239000004310 lactic acid Substances 0.000 claims description 22
- 235000014655 lactic acid Nutrition 0.000 claims description 22
- 239000004520 water soluble gel Substances 0.000 claims description 11
- 239000003292 glue Substances 0.000 claims description 10
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 8
- 239000004820 Pressure-sensitive adhesive Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- 229920002125 Sokalan® Polymers 0.000 claims description 6
- 239000004584 polyacrylic acid Substances 0.000 claims description 6
- 241000186000 Bifidobacterium Species 0.000 claims description 4
- 241000193155 Clostridium botulinum Species 0.000 claims description 4
- 241000193468 Clostridium perfringens Species 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 235000010413 sodium alginate Nutrition 0.000 claims description 3
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 2
- 229920002907 Guar gum Polymers 0.000 claims description 2
- 239000000665 guar gum Substances 0.000 claims description 2
- 235000010417 guar gum Nutrition 0.000 claims description 2
- 229960002154 guar gum Drugs 0.000 claims description 2
- 239000000661 sodium alginate Substances 0.000 claims description 2
- 229940005550 sodium alginate Drugs 0.000 claims description 2
- 229920001285 xanthan gum Polymers 0.000 claims description 2
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 13
- 238000004140 cleaning Methods 0.000 abstract description 2
- 239000010408 film Substances 0.000 description 49
- 238000000034 method Methods 0.000 description 16
- 244000005700 microbiome Species 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 7
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 235000013311 vegetables Nutrition 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 235000005979 Citrus limon Nutrition 0.000 description 2
- 244000131522 Citrus pyriformis Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical class OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 150000004985 diamines Chemical class 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 229940099596 manganese sulfate Drugs 0.000 description 2
- 235000007079 manganese sulphate Nutrition 0.000 description 2
- 239000011702 manganese sulphate Substances 0.000 description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 235000002639 sodium chloride Nutrition 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 235000002722 Dioscorea batatas Nutrition 0.000 description 1
- 235000006536 Dioscorea esculenta Nutrition 0.000 description 1
- 240000001811 Dioscorea oppositifolia Species 0.000 description 1
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 244000300264 Spinacia oleracea Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 239000005030 aluminium foil Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
Abstract
Piece is tested the utility model discloses a kind of anaerobic bacteria.The utility model provides the anaerobic bacteria test piece that the utility model is provided, and test piece includes the culture film for being stained with colour developing cultivating system for being stained with the oxygen absorbing film of oxygen absorbent and being covered on the oxygen absorbing film；Oxygen absorbing film and culture film are bonded in a side to be connected.It is of the present utility model it is demonstrated experimentally that anaerobic bacteria of the present utility model test piece saves operating personnel's cleaning vessel, prepares the work, easy to operate, ready access upon use such as culture medium, disposal discarded object；Anaerobic bacteria test piece of the present utility model can realize the culture detection of anaerobic bacteria without anaerobism equipment, and good repetitiveness, accuracy is high.
Description
Technical field
The utility model is related to biological technical field, particularly belongs to microbial rapid detection field, more particularly to one kind is detested
Oxygen bacterium tests piece.
Background technology
At present, China anaerobic bacteria detection mainly uses National Standard Method, and the sample being inoculated with is trained in anaerobic box or anaerobism box
Support.The method needs tester to do substantial amounts of preparation, including scrubs vessel, prepares culture medium, sterilizing, disposal discarded object
Deng, and high is required to instrument and equipment, it is necessary to which anaerobism equipment is cultivated, technical ability, experience additionally to operator have higher want
Ask.Because detection is more, easily pollution, accuracy as a result is difficult to control.
Test piece simple with its manufacture craft, easy to operate, as a result accurately, the advantages of detection time is short has won vast inspection
The favor of survey personnel.Test piece need not test before preparation, save labour.Sample liquid is directly drawn to be added dropwise in survey
Detection is cultivated in test piece, biochemical identification link is saved, low is required to operator, it is as a result reproducible.
Anaerobic bacteria test piece in the market only has the lactic acid bacteria test piece of Minnesota Mining and Manufacturing Company, and one kind is that 6406 bacterium colonies are total
Number test piece is tested for lactic acid bacteria, but needs tester to prepare MRS nutrient solutions in advance as dilution, and needs anaerobism to train
Support；Another is the 6461 lactic acid bacterias test piece that in August, 2016 is released, and this test piece adds reducing agent, is not required in the medium
Anaerobism equipment is wanted, but accuracy gap compared with conventional is larger, stops selling in the end of the year in 2016.
The content of the invention
A purpose of the present utility model is to provide a kind of anaerobic bacteria test piece.
The anaerobic bacteria test piece that the utility model is provided, anaerobic bacteria test piece includes the oxygen absorbing film for being stained with oxygen absorbent
With the culture film for being stained with colour developing cultivating system being covered on the oxygen absorbing film.
The material of the oxygen absorbing film is by the airtight adhesive tape of waterproof, oxygen absorbent and waterproof and breathable adhesive tape composition.
The oxygen absorbing film for being stained with oxygen absorbent is prepared according to the method comprised the following steps：Oxygen absorbent is adhered into waterproof not
The glue surface of breathable adhesive tape, then waterproof and breathable adhesive tape is sticked to the surface of oxygen absorbent, form the oxygen absorbing film for being stained with oxygen absorbent.
The glue of the glue surface of adhesive tape is polyacrylic acid pressure sensitive adhesive.
The material of culture film of colour developing cultivating system is stained with by chromogenic culture medium, cold water soluble gel and waterproof are not
Breathable adhesive tape is constituted.
The culture film for being stained with colour developing cultivating system is prepared according to the method comprised the following steps：Cold water soluble is coagulated
After glue and the mixing of microorganism chromogenic culture medium, the cultivating system that develops the color is obtained, then the colour developing cultivating system is adhered to described anti-
The surface of the airtight adhesive tape of water, forms the culture film for being stained with colour developing cultivating system.
The glue of the glue surface of adhesive tape is polyacrylic acid pressure sensitive adhesive.
The side of oxygen absorbing film waterproof and breathable adhesive tape face one and the culture film are stained with colour developing cultivating system face
One side is bonded, and can be bonded by polyacrylic acid pressure sensitive adhesive.
In above-mentioned oxygen absorbing film, the airtight adhesive tape of waterproof is PET adhesive tape；
The PET adhesive tape thickness is 0.10mm-0.50mm；
The PET adhesive tape is 7.0-10.0cm × 8.0-11.0cm rectangle；
The waterproof and breathable adhesive tape is PVC tape；
The PVC tape thickness is 0.03mm-0.10mm；
The PVC tape is 7.0-10.0cm × 8.0-11.0cm rectangle.
In above-mentioned oxygen absorbing film, the oxygen absorbent is iron powder；
The iron powder mesh number is the mesh of 120 mesh -200.
In above-mentioned culture film, the airtight adhesive tape of waterproof is PET adhesive tape；
PET adhesive tape thickness, width are with PET adhesive tape in oxygen absorbing film in the culture film, and length is longer than in oxygen absorbing film
PET adhesive tape 0.3cm-0.5cm.
In above-mentioned culture film, the mass ratio of the microorganism chromogenic culture medium and the cold water soluble gel is 1:1-
3。
In above-mentioned anaerobic bacteria test piece, the microorganism chromogenic culture medium is lactic acid bacteria chromogenic culture medium, Bifidobacterium shows
Color culture medium, C.perfringens chromogenic culture medium or clostridium botulinum chromogenic culture medium.
In an embodiment of the present invention, the microorganism chromogenic culture medium used is lactic acid bacteria chromogenic culture medium, and it is trained by MRS
Support base and colour developing thing TTC is constituted, the mass ratio of the colour developing thing TTC and the MRS culture mediums is specially 0.05:1.MRS is cultivated
Base is 18.88% beef protein powder by mass fraction；18.88% flesh of fish juice；9.44% Yeast diffusion juice powder；37.76% glucose；
9.44% sodium acetate；3.78% lemon acid diamine；0.19% Tween 80；1.10% magnesium sulfate and 0.53% manganese sulfate composition.
In above-mentioned culture film, the cold water soluble gel is that sodium alginate, xanthans, guar gum or methylol are fine
One kind in the plain sodium of dimension.
The utility model also provides the method that the anaerobic bacteria tests piece for preparing, and comprises the following steps：
The step of preparation test piece, includes：
1）Prepare the oxygen absorbing film for being stained with oxygen absorbent
The oxygen absorbing film for being stained with oxygen absorbent is the glue surface that oxygen absorbent is adhered to the airtight adhesive tape of waterproof, then will be anti-
Water breathable adhesive tape sticks to the surface of oxygen absorbent, forms the oxygen absorbing film for being stained with oxygen absorbent；
2）Prepare the culture film for being stained with colour developing cultivating system
The culture film for being stained with colour developing cultivating system is to mix cold water soluble gel and microorganism chromogenic culture medium
Afterwards, the cultivating system that develops the color is obtained, then the colour developing cultivating system is adhered into the surface of the airtight adhesive tape of the waterproof, forms viscous
With the culture film of colour developing cultivating system
3）Assembling
The oxygen absorbing film waterproof and breathable adhesive tape face and the culture film are stained with colour developing cultivating system face in short side
Side is bonded, and can be bonded by polyacrylic acid pressure sensitive adhesive.
Application in above-mentioned anaerobic bacteria test piece detection anaerobic bacteria limit is also the scope of the utility model protection；
Or, the application in above-mentioned anaerobic bacteria test piece progress anaerobic bacteria counting is also the scope of the utility model protection.
Above-mentioned anaerobic bacteria is lactic acid bacteria, Bifidobacterium, at least one of C.perfringens or clostridium botulinum.
The utility model provides a kind of method that anaerobic bacteria counts, and comprises the following steps：
1）The culture film that above-mentioned anaerobic bacteria tests piece is uncovered, then microorganism sample liquid to be checked is added in the test piece
On oxygen absorbing film, the culture film is covered, makes sample liquid free diffusing, piece is tested after being loaded；
2）Piece will be tested after the sample-adding and is placed in culture in incubator, bacterium colony after culture will be counted.
It is of the present utility model it is demonstrated experimentally that anaerobic bacteria of the present utility model test piece save operating personnel's cleaning vessel,
The work such as culture medium, disposal discarded object are prepared, easy to operate, ready access upon use, good repetitiveness, accuracy is high.This practicality is new
Type introduces test piece using iron powder as oxygen absorbent first, it is not necessary to which anaerobism equipment can realize the detection of anaerobic bacteria, successfully break through
Limitation of the traditional detection in terms of instrument and equipment；Culture is used as on adhesive tape using being sticked at after the directly mixing of chromogenic culture medium and gel
Layer, saves culture medium and adds water the links such as allotment, coating, drying, destruction of the reduction manufacture craft to nutritional ingredient；Manufacture craft letter
It is single；Test piece high with conventional detection coincidence rate, as a result accurately.
Brief description of the drawings
Fig. 1 is the product figure that anaerobic bacteria tests piece.
Fig. 2 is the lateral effect figure that anaerobic bacteria tests piece.
Embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
Material, reagent used etc., unless otherwise specified, are commercially obtained in following embodiments.
Embodiment 1, anaerobic bacteria test piece
The structural representation of anaerobic bacteria test piece of the present utility model is as depicted in figs. 1 and 2.
Anaerobic bacteria test piece shown in Fig. 1 and Fig. 2, including be stained with the oxygen absorbing film of oxygen absorbent and be stained with colour developing culture
The culture film of system；Oxygen absorbing film includes the airtight adhesive tape 1 of waterproof, oxygen absorbent 2 and waterproof and breathable adhesive tape 3；Cultivate film bag
Include colour developing cultivating system 4 and the airtight adhesive tape 5 of waterproof；Oxygen absorbing film waterproof and breathable adhesive tape face and the culture film are stained with aobvious
Color cultivating system face bonds in short side side and connected.
It is stained with the oxygen absorbing film of oxygen absorbent oxygen absorbent is adhered to the glue surface of the airtight adhesive tape of waterproof, then waterproof is saturating
Gas adhesive tape sticks to the surface of oxygen absorbent, forms the oxygen absorbing film for being stained with oxygen absorbent.
The culture film for being stained with colour developing cultivating system is to mix cold water soluble gel and microorganism chromogenic culture medium
Afterwards, the cultivating system that develops the color is obtained, then the cultivating system that develops the color is adhered into the glue surface of adhesive tape, is formed and is stained with colour developing cultivating system
Cultivate film.
Colour developing cultivating system is made up of microorganism chromogenic culture medium and cold water soluble gel, wherein, microorganism colour developing training
The mass ratio for supporting base and cold water soluble gel is 1:1-3.
Microorganism chromogenic culture medium is lactic acid bacteria chromogenic culture medium, Bifidobacterium chromogenic culture medium, C.perfringens show
Color culture medium or clostridium botulinum chromogenic culture medium.
Above-described embodiment is merely to illustrate the utility model, wherein the structure of each part, set location and its connected mode
Deng can all be varied from, every equivalents carried out on the basis of technical solutions of the utility model and improvement,
It should not exclude outside protection domain of the present utility model.
Embodiment 2, lactic acid bacteria test the preparation and application of piece
First, lactic acid bacteria tests the preparation of piece
1st, it is stained with the preparation of the oxygen absorbing film of oxygen absorbent
By iron powder（Changzhou Zhi Guang activated carbons Co., Ltd, 200 mesh）Adhere to PET adhesive tape（Shenzhen He Chang makes the science and technology prosperous limited
Company；Article No.：401, thickness 0.11mm）Surface, then by PVC tape（Yangzhou Teng Long packaging materials Co., Ltd；Thickness
0.03mm）Iron powder surface is adhered to, obtains being stained with the oxygen absorbing film of iron powder.
2nd, it is stained with the preparation of the culture film of colour developing cultivating system
By 2.00g sodium alginates（Henan De Wang chemical industry Industrial Co., Ltd.；Article No.：9005-38-3）It is aobvious with 1.05g MRS
Color culture medium is well mixed, and obtains the cultivating system that develops the color, then colour developing cultivating system is adhered into PET adhesive tape（Shenzhen He Chang Xing Ke
Skill Co., Ltd；Article No.：401, thickness 0.11mm）Surface, obtains being stained with the culture film of colour developing cultivating system.
Above-mentioned MRS chromogenic culture mediums are by 1.00g MRS culture mediums and 0.05g indicator TTC（Sigma companies article No.
T8877）It is uniformly mixed so as to obtain；MRS culture mediums are 18.88% beef protein powder by mass fraction；18.88% flesh of fish juice；9.44% yeast soaks
Juice powder；37.76% glucose；9.44% sodium acetate；3.78% lemon acid diamine；0.19% Tween 80；1.10% magnesium sulfate and
0.53% manganese sulfate is constituted.
3rd, the assembling of piece is tested
Above-mentioned 1 oxygen absorbing film for being stained with iron powder prepared is cut into 9.0cm × 10.0cm rectangle；
Rectangle of the culture film slitting for being stained with colour developing cultivating system that above-mentioned 2 are prepared into 9.0cm × 10.5cm；
Above-mentioned oxygen absorbing film waterproof and breathable adhesive tape face and the culture film are stained with colour developing cultivating system face in short side
Side is bonded with pressure sensitive adhesive, bonding width 1.0cm.
It is standby after packaging of aluminium foil bag, ethylene oxide sterilizing, obtain the lactic acid bacteria test piece shown in Fig. 1 or Fig. 2.
2nd, lactic acid bacteria test piece detection lactic acid bacteria sum
1st, lactic acid bacteria test piece detection method
Inoculation：6 parts of raw vegetable samples and 4 parts of active lactic acid bacteria drink samples are taken respectively, and every part of sample presses sterile working
25g is taken in sterile homogenizing bag, 225mL sterile salines is added, with slap type homogenizer homogeneous 1min, is made 10-1Bacterium solution,
1mL is drawn again to be added in 9mL sterile salines, is made 10-2Bacterium solution, 10 times of gradient dilutions are done with this.Select 2-3 individual suitable dilute
Degree of releasing respectively draws 1mL and the oxygen absorbing film surface that piece is tested in above-mentioned one obtained lactic acid bacteria is added dropwise, and slowly covers upper strata culture thin
Film, makes sample liquid free diffusing, and piece is tested after being loaded, and solidifies 1min, each 2 repetitions of dilution factor.Draw 1mL sterile physiologicals
Salt solution is added dropwise tests piece as blank control, 2 repetitions of blank control in above-mentioned one obtained lactic acid bacteria.
Culture：Stack every pile and be no more than 10,36 DEG C ± 1 DEG C aerobic culture 48h ± 2h.
Count：Culture counts red colonies after terminating, and 2 test piece average values with average in 20-200 bacterium colony multiply
It is total using corresponding dilution factor as the sample lactic acid bacteria.Blank control clump count is 0, and it is invalid otherwise to test.
2nd, MRS flat band methods
2nd, MRS flat band methods
It is inoculated with, cultivated and counted by GB4789.35-2010 method.
Statistics：The each sample total plate count measured with testing piece method and MRS flat band methods does t- inspections, exists with P ＜ 0.05
Significant difference.
Table 1 detects 6 parts of raw vegetable samples and 4 parts of active lactic acid bacteria drink sample breasts for test piece method and MRS flat band methods
The total result of sour bacterium
（Unit：cfu/g）
——————————————————————————————————————
———————————————
Detection method 123456789 10
——————————————————————————————————————
———————————————
Test piece 2.0 × 108 1.6×108 4.5×107 1.3×102 1.9×103 5.6×104 9.3
×103 7.1×103 2.4×102 1.5×103
MRS flat band methods 2.5 × 108 1.2×108 5.3×107 1.0×102 1.5×103 4.7×104
8.2×103 6.4×103 2.0×102 1.0×103
——————————————————————————————————————
———————————————
Note：1：The excellent beneficial C of Mongolia Ox；2：Erie's yoghurt 3：Mongolia Ox's hat benefit breast；4：Ternary fresh milk；5：Tomato；6：Ma Ling
Potato；7：Spinach；8：Chinese yam； 9：Cucumber；10：Eggplant
Experiment proves that test piece method detects 6 parts of raw vegetable samples and 4 parts of active lactic acid bacteria drink samples with MRS flat band methods
Lactic acid bacteria sum is without significant difference（The ＞ 0.05 of P=0.48）, test piece method can be total instead of lactic acid bacteria in conventional method sample survey
Number.
Claims (7)
1. a kind of anaerobic bacteria tests piece, anaerobic bacteria test piece includes being stained with the oxygen absorbing film of oxygen absorbent and is covered in the suction
The culture film for being stained with colour developing cultivating system on oxygen film；
The material of the oxygen absorbing film is by the airtight adhesive tape of waterproof, oxygen absorbent and waterproof and breathable adhesive tape composition；
The material of the culture film for being stained with colour developing cultivating system is by chromogenic culture medium, and cold water soluble gel and waterproof are not
Breathable adhesive tape is constituted.
2. anaerobic bacteria according to claim 1 tests piece, it is characterised in that：
Oxygen absorbing film is stained with oxygen absorbent.
3. anaerobic bacteria according to claim 1 tests piece, it is characterised in that：
The oxygen absorbing film for being stained with oxygen absorbent is that oxygen absorbent adhered into the airtight tape surface of waterproof, then by waterproof and breathable adhesive tape
Oxygen absorbent surface is adhered to, the oxygen absorbing film for being stained with oxygen absorbent is formed.
4. anaerobic bacteria according to claim 1 tests piece, it is characterised in that：
The culture film for being stained with colour developing cultivating system is to mix cold water soluble gel and anaerobic bacteria chromogenic culture medium
Afterwards, the cultivating system that develops the color is obtained, then the colour developing cultivating system is adhered into the glue surface of the airtight adhesive tape of waterproof, formation is stained with
The culture film of colour developing cultivating system.
5. piece is tested according to any described anaerobic bacteria in claim 1-4, it is characterised in that：
The side of oxygen absorbing film one is connected with the side bonding of the culture film.
6. piece is tested according to any described anaerobic bacteria in claim 1-4, it is characterised in that：
The material of the airtight adhesive tape of waterproof is specially PET polyacrylic acid pressure sensitive adhesive adhesive tapes；
The material of the waterproof and breathable adhesive tape is specially PVC polyacrylic acid pressure sensitive adhesive adhesive tapes；
The oxygen absorbent is iron powder；
The cold water soluble gel is specially sodium alginate, xanthans, guar gum or sodium cellulose glycolate；
The chromogenic culture medium is lactic acid bacteria chromogenic culture medium, Bifidobacterium chromogenic culture medium, C.perfringens colour developing culture
One kind in base or clostridium botulinum chromogenic culture medium.
7. test piece according to claim 4, it is characterised in that：
The mass ratio of the chromogenic culture medium and the cold water soluble gel is 1:1-3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720297465.7U CN206616224U (en) | 2017-03-24 | 2017-03-24 | A kind of anaerobic bacteria tests piece |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720297465.7U CN206616224U (en) | 2017-03-24 | 2017-03-24 | A kind of anaerobic bacteria tests piece |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206616224U true CN206616224U (en) | 2017-11-07 |
Family
ID=60233999
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| Application Number | Title | Priority Date | Filing Date |
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| CN201720297465.7U Expired - Fee Related CN206616224U (en) | 2017-03-24 | 2017-03-24 | A kind of anaerobic bacteria tests piece |
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| CN (1) | CN206616224U (en) |
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| GR01 | Patent grant | ||
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Granted publication date: 20171107 |