CN206580823U - A kind of device for filtering and collecting of cast-off cells - Google Patents
A kind of device for filtering and collecting of cast-off cells Download PDFInfo
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- CN206580823U CN206580823U CN201720159787.5U CN201720159787U CN206580823U CN 206580823 U CN206580823 U CN 206580823U CN 201720159787 U CN201720159787 U CN 201720159787U CN 206580823 U CN206580823 U CN 206580823U
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- filter membrane
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Abstract
A kind of device for filtering and collecting of cast-off cells, the filter is provided with the separator that disposably cast-off cells are separated out, the separator includes the pillar hollow cylinder for being provided with four layers of filter membrane in it from top to bottom, and four layers of filter membrane is followed successively by the first filter membrane, the second filter membrane, the 3rd filter membrane and the 4th filter membrane according to the order arranged from top to bottom.Effectively prevent occur in the prior art complex steps, experiment operator take a lot of trouble arduously, influence experiment uniformity, easily cause cross pollution, cause experimental error and be difficult to meet the defect of high flux requirement.
Description
Technical field
The utility model is related to medical science vitro diagnostic techniques field, and in particular to a kind of cast-off cells are collected by filtration
Cast-off cells collection device in device, specifically excrement, is especially adapted for use in colorectal cancer patients excrement midgut wall and comes off
The collection device of cell, it is according to elutriation method, by designing a kind of pillar filter plant that four metafiltration membrane structures are housed, and at it
Two ends are respectively equipped with fecal specimens sample holes and cell collects outlet, realize the collection of cast-off cells in excrement.
Background technology
Colorectal cancer is as main malignant tumor of digestive tract, with higher fatal rate.Colorectal cancer is carried out timely
Diagnosis and treatment be improve survival key factor, especially early diagnosis can significantly improve the survival rate of patient,
Clinically have great importance.Conventional diagnosis of colorectal carcinoma method includes at present：Fibersigmoidoscopy, excrement
Occult blood test and total colonoscopy, wherein occult blood test are current generaI investigation most common methods.
In recent years, with the development of technology, some new detection modes start to occur, such as the excrement based on gene level
Cast-off cells are detected in middle DNA detections and the excrement based on cellular level.These methods have noninvasive and detection specificity is higher
The characteristics of, the extraction of cast-off cells in excrement can be related to during detection.The detection object of fecal cast-off cell detection is straight
Connect the cast-off cells exactly extracted through excrement；DNA is detected in excrement, sometimes to improve the accuracy detected, can also be used and first be carried
Cast-off cells are taken, cell DNA is then extracted, the mode of DNA detections is finally carried out.
Fecal specimens complicated component, intestinal wall cast-off cells only account for few part, and the presence of other compositions, such as enteron aisle are thin
Polysaccharide and cholate in bacterium, mucus, swill etc., can influence final testing result.Therefore, come off in excrement thin
When born of the same parents are extracted, other impurities must be removed as far as possible.The existing method that cast-off cells are extracted from excrement has：Density level bands
Centrifugal process, elutriation method, immunomagnetic beads method are spent, wherein elutriation method is more commonly used.The key of elutriation method is four filterings, complete in four times
Into aperture of filter screen is sequentially reduced, and the washing device that the method is used is small to primary cellular defect and recovery rate is high, but there is also necessarily not
Foot, existing washing device has the disadvantage that when especially carrying out the clinical detection of a large amount of samples：
1. there are complex steps, the problem of experiment operator takes a lot of trouble laborious using existing washing device；
2. four filterings are generally required using existing washing device, manual operation is excessive, influence experiment uniformity, Yi Zao
Into cross pollution, cause experimental error；
3. using existing washing device, by experimental implementation, time-consuming, it is difficult to meet high flux requirement.
The content of the invention
To solve the above problems, the utility model provides a kind of device for filtering and collecting of cast-off cells, it effectively prevent
Occur in the prior art complex steps, experiment operator take a lot of trouble arduously, influence experiment uniformity, easily cause cross pollution, lead
Cause experimental error and it is difficult to meet the defect of high flux requirement.
In order to overcome deficiency of the prior art, the utility model provides a kind of device for filtering and collecting of cast-off cells
Solution, it is specific as follows：
A kind of device for filtering and collecting of cast-off cells, including the filter for cast-off cells, the filter are set
The separator that once property separates out cast-off cells.
Further, the separator includes the pillar hollow cylinder for being provided with four layers of filter membrane in it from top to bottom,
Four layers of filter membrane according to the order arranged from top to bottom be followed successively by the first filter membrane, the second filter membrane, the 3rd filter membrane and
4th filter membrane.
Further, the hollow cylinder is with the sample holes for input sample and for collecting and sending out cast-off cells
Outlet is collected to be connected.
Further, the filter sizes of first filter membrane are 600 μm, and the filter sizes of second filter membrane are
150 μm, the filter sizes of the 3rd filter membrane are 75 μm, and the filter sizes of the 4th filter membrane are 50 μm.
Further, the filter opening of four layers of filter membrane uses circumference layout structure.
The beneficial effects of the utility model are：
Can in the detection of supporting cast-off cells suitable for excrement and excrement cast-off cells DNA detections, can facilitate to excrement
The processing of concentration filter of sample, cast-off cells are provided for the subsequent step of analyzing and diagnosing, with simple to operate, integrated filtering,
Easy to use, small to the primary cellular defect, advantage that recovery rate is high.
Brief description of the drawings
Fig. 1 is the outline plan schematic diagram of the device for filtering and collecting of the utility model cast-off cells.
Fig. 2 is the inside dimensional structure diagram of the device for filtering and collecting of the utility model cast-off cells.
Fig. 3 is the filter opening distribution schematic diagram of the device for filtering and collecting of the utility model cast-off cells.
Embodiment
, during applied to clinic, there is objective shortcoming, such as step is numerous in the device that cast-off cells are collected in current existing excrement
Trivial, manual operation is excessive, and the infringement to cell is larger, and cell extraction rate is low.This practicality is devised in view of above mentioned problem new
Type, the utility model can support the use cast-off cells detection and the DNA detections of cast-off cells in excrement in excrement, be carried for detection
For cast-off cells sample.
The utility model is described further below in conjunction with drawings and examples.
Embodiment 1
1- Fig. 3 is understood with reference to the accompanying drawings, the device for filtering and collecting of the cast-off cells of the present embodiment, and cast-off cells are collected by filtration
The solution of device, it is specific as follows：
The device for filtering and collecting of cast-off cells, including the filter for cast-off cells, the filter are provided with one
The separator that secondary property separates out cast-off cells.
The separator includes the pillar hollow cylinder 2 for being provided with four layers of filter membrane in it from top to bottom, described four layers
Filter membrane is followed successively by the first filter membrane 4, the second filter membrane 5, the 3rd filter membrane 6 and the 4th mistake according to the order arranged from top to bottom
Filter membrane 7.
The hollow cylinder is exported with the sample holes 1 for input sample and for the collection for collecting and sending out cast-off cells
3 are connected.The sample is fecal specimens.
The fecal specimens suspension that so buffered liquid is handled injects pillar hollow cylinder 2 from sample holes 1；Fecal specimens
The first filter membrane 4, the second filter membrane 5, the 3rd filter membrane 6 and the 4th filter membrane 7 are sequentially passed through, final filtration obtains coming off carefully
Born of the same parents；The cast-off cells being filtrated to get are collected through collecting outlet 3.
Embodiment 2
1- Fig. 3 is understood with reference to the accompanying drawings, the device for filtering and collecting of the cast-off cells of the present embodiment, and the filter is provided with one
The separator that secondary property separates out cast-off cells.
The separator includes the pillar hollow cylinder 2 for being provided with four layers of filter membrane in it from top to bottom, described four layers
Filter membrane is followed successively by the first filter membrane 4, the second filter membrane 5, the 3rd filter membrane 6 and the 4th mistake according to the order arranged from top to bottom
Filter membrane 7.
The hollow cylinder is exported with the sample holes 1 for input sample and for the collection for collecting and sending out cast-off cells
3 are connected.The sample is fecal specimens.
The filter sizes of first filter membrane 4 are 600 μm, and the filter sizes of second filter membrane 5 are 150 μm, institute
The filter sizes for stating the 3rd filter membrane 6 are 75 μm, and the filter sizes of the 4th filter membrane 7 are 50 μm.
The fecal specimens suspension that so buffered liquid is handled injects pillar hollow cylinder 2 from sample holes 1；Fecal specimens
Sequentially pass through the first filter membrane 4, the second filter membrane 5, the 3rd filter membrane 6 and the 4th filter membrane 7, the first filter membrane 4 and the second mistake
The pore size of the filter opening of filter membrane 5 is respectively 600 μm and 150 μm, and aperture is larger, can carry out coarse filtration to swill, filter out
The swill of larger particles；The pore size of the filter opening of 3rd filter membrane 6 and the 4th filter membrane 7 is respectively 75 μm and 50 μm,
Aperture is smaller, can filter out the swill of smaller particle with swill fine filtering.Final filtration obtains cast-off cells；Filter
To cast-off cells 3 be collected through collecting outlet.
Embodiment 3
1- Fig. 3 is understood with reference to the accompanying drawings, the device for filtering and collecting of the cast-off cells of the present embodiment, and the filter is provided with one
The separator that secondary property separates out cast-off cells.
The separator includes the pillar hollow cylinder 2 for being provided with four layers of filter membrane in it from top to bottom, described four layers
Filter membrane is followed successively by the first filter membrane 4, the second filter membrane 5, the 3rd filter membrane 6 and the 4th mistake according to the order arranged from top to bottom
Filter membrane 7.
The hollow cylinder is exported with the sample holes 1 for input sample and for the collection for collecting and sending out cast-off cells
3 are connected.The sample is fecal specimens.
The filter sizes of first filter membrane 4 are 600 μm, and the filter sizes of second filter membrane 5 are 150 μm, institute
The filter sizes for stating the 3rd filter membrane 6 are 75 μm, and the filter sizes of the 4th filter membrane 7 are 50 μm.
The filter opening of four layers of filter membrane uses circumference layout structure 8.
The other sample holes 1 are cylindric and are arranged on the top of pillar hollow cylinder 2, and the outlet 3 of collecting is cylinder
Shape is simultaneously arranged on the bottom of pillar hollow cylinder 2.
The fecal specimens suspension that so buffered liquid is handled injects pillar hollow cylinder 2 from sample holes 1；Fecal specimens
Sequentially pass through the first filter membrane 4, the second filter membrane 5, the 3rd filter membrane 6 and the 4th filter membrane 7, the first filter membrane 4 and the second mistake
The pore size of the filter opening of filter membrane 5 is respectively 600 μm and 150 μm, and aperture is larger, can carry out coarse filtration to swill, filter out
The swill of larger particles；The pore size of the filter opening of 3rd filter membrane 6 and the 4th filter membrane 7 is respectively 75 μm and 50 μm,
Aperture is smaller, can filter out the swill of smaller particle with swill fine filtering.Final filtration obtains cast-off cells；Filter
To cast-off cells 3 be collected through collecting outlet.
In summary, the beneficial effects of the utility model are as follows：
Cast-off cells detection and the DNA detections of cast-off cells in excrement in excrement can be supported the use, provides de- for detection
Fall cell sample.Realize it is simple, integrated, conveniently, the extraction to cast-off cells in excrement of small loss.
Compared with prior art or device, technical characterstic of the invention has：
1. integrated filtration treatment, simplifies experimentation, it is laborsaving to be that experiment operator is saved worry；
2. four filter operations encapsulate step completion in the present apparatus, it is to avoid the cross pollution in experimentation；
3. filter process is integrated, it is easy to accomplish automatic business processing, greatly shorten the experiment used time, meet high flux detection need
Ask.
The utility model is described in the way of brief description of the drawings above, it will be understood by those of skill in the art that this
It is open to be not limited to embodiments described above, in the case of without departing from scope of the present utility model, can make a variety of changes,
Change and replace.
Claims (4)
1. a kind of device for filtering and collecting of cast-off cells, including the filter for cast-off cells, it is characterised in that the mistake
Filter device is provided with the separator that disposably cast-off cells are separated out, and the separator includes being provided with four from top to bottom in it
The pillar hollow cylinder of layer filter membrane.
2. the device for filtering and collecting of cast-off cells according to claim 1, it is characterised in that the hollow cylinder is with being used for
The sample holes of input sample are exported with the collection for collecting and sending out cast-off cells to be connected.
3. the device for filtering and collecting of cast-off cells according to claim 1, it is characterised in that four layers of filter membrane according to
The order arranged from top to bottom is followed successively by the first filter membrane, the second filter membrane, the 3rd filter membrane and the 4th filter membrane, described first
The filter sizes of filter membrane are 600 μm, and the filter sizes of second filter membrane are 150 μm, the filter membrane of the 3rd filter membrane
Aperture is 75 μm, and the filter sizes of the 4th filter membrane are 50 μm.
4. the device for filtering and collecting of cast-off cells according to claim 1, it is characterised in that the filter of four layers of filter membrane
Hole uses circumference layout structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720159787.5U CN206580823U (en) | 2017-02-22 | 2017-02-22 | A kind of device for filtering and collecting of cast-off cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720159787.5U CN206580823U (en) | 2017-02-22 | 2017-02-22 | A kind of device for filtering and collecting of cast-off cells |
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| Publication Number | Publication Date |
|---|---|
| CN206580823U true CN206580823U (en) | 2017-10-24 |
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ID=60090851
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720159787.5U Active CN206580823U (en) | 2017-02-22 | 2017-02-22 | A kind of device for filtering and collecting of cast-off cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206580823U (en) |
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2017
- 2017-02-22 CN CN201720159787.5U patent/CN206580823U/en active Active
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