CN206549664U - A kind of multiple determination micro-fluidic chip - Google Patents
A kind of multiple determination micro-fluidic chip Download PDFInfo
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- CN206549664U CN206549664U CN201720153206.7U CN201720153206U CN206549664U CN 206549664 U CN206549664 U CN 206549664U CN 201720153206 U CN201720153206 U CN 201720153206U CN 206549664 U CN206549664 U CN 206549664U
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Abstract
The utility model discloses a kind of multiple determination micro-fluidic chip, it is related to microfluidic chip analysis field.The chip includes base and upper lid, and base includes sample reception pond, shunting pond, several reaction tanks and the waste liquid pool being sequentially communicated, and reaction tank is connected by detect tank with waste liquid pool；Upper lid include being arranged on the injection port of sample reception pond opposite position, be arranged on the driver plate corresponding with shunting pond position, driver plate includes the inlet and outlet in rotating disk, handle and rotating disk circumference, after upper lid and bottom seat bonding, shunting pond can be chimeric with driver plate, import is connected with sample reception pond, and outlet is selectively connected by rotating rotating disk with reaction tank.The beneficial effects of the utility model are can not only to quantify control to participate in the sample size of reaction, and sample can be controlled to participate in the sequencing of reaction, and sample can also be controlled to be selectively entered reaction tank and reacted, easy, efficient multiple determination is realized.
Description
Technical field
The utility model is related to microfluidic chip analysis field, and in particular to be a kind of micro-fluidic core of multiple determination
Piece.
Background technology
Microfluidic chip technology is bases such as biology, chemistry, the sample preparation of medical analysis process, reaction, separation, detections
This operating unit is integrated on the chip of one piece of micro-meter scale, is automatically performed analysis total system.Because it is in biological, chemistry, doctor
The great potential in etc. field, and develop into a biology, chemistry, medical science, fluid, electronics, material, mechanical subject intersection
Brand-new research field.The characteristics of this microfluidic chip technology has efficiency high (high analyte, low cost), easily popularized.
When carrying out multiple determination to same sample, same sample needs to carry out multiple detection reactions, takes relatively long, and inspection
Survey process is cumbersome.In the prior art, micro-fluidic chip is applied to multi objective biochemistry detection by someone, by being set on chip
Multiple reaction tanks occurred for detection reaction are detected to same sample, so as to realize that multiple indexs can be achieved in single injected sampling
Detection reaction.
Authorization Notice No. discloses a kind of multiple determination miniflow quantitatively shunted for CN 205127986U Chinese patent
Chip is controlled, the chip includes underlying basal and upper strata cover plate, bond together to form sheet chip, cover plate is provided with injection port, substrate
Provided with multi-stage diffluence branch road and reaction tank, one end of size identical reaction tank connects corresponding each diverter branch, and the other end leads to
Cross fluid cushion passage to connect with multiple buffer pools, contacted between buffer pool, the size of buffer pool with liquid flow direction successively
Reduce, fluid cushion channel end is connected with the gas outlet on cover plate.Although the chip can be realized respectively by quantitative shunting
After sample size in parallel reaction pond is identical, but the chip is single injected sampling, sample enters all parallel reactions simultaneously
React in pond and simultaneously, the sequencing that sample can not be controlled to enter in each parallel reaction pond can not control sample
Some or certain several reaction tanks are only selectively entered individually to react.
Utility model content
Technical problem to be solved in the utility model is the shortcoming for overcoming above prior art：A kind of multi objective is provided
Detection micro-fluidic chip, can not only quantify control and enter the sample size of reaction tank participation reaction, and can control sample
Optionally only enter some or certain several reaction tanks individually to react, sample can also be controlled to be reacted into reaction tank
Sequencing, realize easy, efficient multiple determination.
Technical solution of the present utility model is as follows：A kind of multiple determination micro-fluidic chip, including base and upper
Lid, described base includes sample reception pond, shunting pond, several reaction tanks and the waste liquid pool being sequentially communicated, and described is anti-
Ying Chi is connected by detect tank with waste liquid pool；Described upper lid includes being arranged on the sample introduction with sample reception pond opposite position
Mouthful, be arranged on the corresponding driver plate in shunting pond position, the import including rotating disk, handle and in rotating disk circumference of described driver plate and
Outlet, after upper lid and bottom seat bonding, shunting pond can be chimeric with driver plate, and described import is connected with sample reception pond, the outlet
Connected by rotating rotating disk with reaction tank.
The utility model, by driver plate with shunting using cooperatively for pond, can play to enter sample by setting dial structure
The effect that row is quantitatively shunted, by the handle on rotating driver plate, can connect the outlet on rotating disk with any reaction tank, not only
Control can be quantified and enter the sample size of reaction tank participation reaction, and sample can be controlled optionally to only enter some or certain
Several reaction tanks are reacted, i.e., optionally only detect some indexs, and are detected together without being confined to all indexs, real
The selectivity of Testing index is showed, so when sample size is less, the consumption of sample can be saved；It can also control simultaneously
Sample enters the sequencing of reaction tank, so as to be controlled according to the difference reacted in reaction tank in the different reaction time,
To obtain optimal reaction result.
Further, the bottom of the base is provided with for regulation to be heated and can needed according to reaction to reaction tank
The electric heating sheets of heating-up temperature, the quantity of described electric heating sheets is equal with reaction tank quantity, respectively positioned at reaction tank just under
Side.
Preferably, the size of described electric heating sheets is identical with reaction tank.
The utility model can adjust the electric heating sheets of temperature by being provided with the underface of reaction tank, you can with basis
The different controls of reaction not only contribute to the progress of reaction in reaction tank, improve reaction in different heating-up temperatures in reaction tank
Speed and the efficiency of detection, and can be by controlling reaction tank to keep optimal reaction temperature to reach optimal reaction knot
Really, the accuracy of reaction is improved, is conducive to the accurate analysis of multiple determination result.
Described upper lid includes the steam vent for being arranged on waste liquid pool and sample reception pond opposite position.
The sample reception pond is that, provided with fixed filter pad in isosceles trapezoid, sample reception pond, described filter pad is
Prepared by semipermeable membrane material, its shape and sample reception pond are adapted.
Described reaction tank is advance fixed many indexes detection reagent in size identical ellipse, reaction tank, for example
Enzyme and substrate, antigen or antibody and PCR primer etc., can pass through physical absorption, chemical crosslinking, sol-gel embedding, microballon
The modes such as fixed or film fixation are fixed；
The reaction tank is preferably 3~6.
Described detect tank is size identical gallery, is fixed with Multiple Antibodies in advance in detect tank.
The base and upper lid for the multiple determination micro-fluidic chip that the utility model is provided can select poly dimethyl silicon
One kind in oxygen alkane (PDMS), polymethyl methacrylate (PMMA), makrolon (PC).Base and upper lid can pass through hot pressing
Any mode is bonded together in bonding, laser bonding, ultrasonic wave added bonding, so as to realize the closed sealing-in of chip.
Brief description of the drawings
Fig. 1 is the micro-fluidic chip base schematic diagram for three kinds of Indexs measures.
Fig. 2 is the lid schematic diagram on the micro-fluidic chip of three kinds of Indexs measures.
Fig. 3 is the sectional view in the AA directions along along Fig. 1 and Fig. 2.
Fig. 4 is the micro-fluidic chip overall structure diagram for three kinds of Indexs measures.
Fig. 5 is the micro-fluidic chip overall structure diagram for three kinds of Indexs measures.
1 is sample reception pond, and 2 be shunting pond, and 21 be limiter, and 3 be reaction tank, and 4 be detect tank, and 5 be waste liquid pool, and 6 are
Electric heating sheets, 7 be injection port, and 8 be driver plate, and 81 be rotating disk, and 82 be handle, and 83 be import, and 84 be opening, and 9 be steam vent.
Embodiment
The utility model is described in further details with specific embodiment below, but the utility model be not only limited to
Lower specific embodiment.
Embodiment one
A kind of to be used for the micro-fluidic chip of three kinds of Indexs measures as shown in Fig. 1,2,3,4,5, the micro-fluidic chip is the bottom of by
The plates that seat 30 and upper lid 20 are bonded.Base 30 include be sequentially communicated sample reception pond 1, shunting pond 2, reaction tank 3,
Detect tank 4, waste liquid pool 5, and it is 3 to be arranged on reaction tank 3 in the electric heating sheets 6 of the bottom of base 30, the present embodiment, 3 are anti-
Answer pond 3 in parallel, one end of reaction tank 3 is connected with shunting pond 2 respectively, and the other end is respectively communicated with a detect tank 4, passes through detect tank
4 connect with a waste liquid pool 5.Upper lid 20 include be arranged on the injection port 7 corresponding with the position of sample reception pond 1, be arranged on
Shunt the corresponding driver plate 8 in the position of pond 2 and be arranged on the steam vent 9 corresponding with sample reception pond 1 and the position of waste liquid pool 5,
Driver plate 8 includes an import 83 and one outlet 84 in rotating disk 81, handle 82 and rotating disk circumference, by base 30 and the key of upper lid 20
After conjunction, shunting pond 2 is then chimeric with driver plate 8 to form an entirety, and import 83 is connected with sample reception pond 1, passes through rotating driver plate
8, the selectivity of outlet 84 is connected with any reaction tank 3.
The angle of import 83 on driver plate 8 is consistently greater than the angle between the entrance of reaction tank 3 of most both sides, so as to
To ensure that import 83 can be connected with sample reception pond 1 all the time during rotating driver plate 8 makes outlet 84 be connected with shunting pond 2.
In the present embodiment, sample reception pond 1 is to be fixed with filter pad in isosceles trapezoid, sample reception pond 1, and filter pad is
Prepared by semipermeable membrane material, its shape and sample reception pond are adapted, for being filtered to sample.
Reaction tank 3 is preferably oval for size identical in the present embodiment, fixes many indexes inspection in reaction tank 3 in advance
Test agent, such as enzyme and substrate, antigen or antibody and PCR primer, can pass through physical absorption, chemical crosslinking, colloidal sol-solidifying
Glue embedding, microballon fix or film fix etc. mode be fixed, sample enter reaction tank after, enter with the detection reagent in reaction tank
Row reaction.
Detect tank 4 is size identical gallery in the present embodiment, wherein being fixed with Multiple Antibodies in advance；The present embodiment
In waste liquid pool 5 to be square, for collecting waste liquid.
In the present embodiment, electric heating sheets 6 are arranged at the bottom of base 30, and positioned at the underface of reaction tank 3, for anti-
Answer pond 3 to be heated and can need to adjust heating-up temperature according to reaction, the size and number of electric heating sheets 6 respectively with reaction
Pond 3 is identical.
The utility model, by driver plate 8 with shunting using cooperatively for pond 2, can be played to sample by setting the structure of driver plate 8
The effect quantitatively shunted, by the handle on rotating driver plate, can connect the outlet on rotating disk with any reaction tank, no
Only can quantify control enter reaction tank participate in reaction sample size, and can control sample optionally only enter some or
Certain several reaction tank is reacted, i.e., optionally only detect some indexs, and is detected together without being confined to all indexs,
The selectivity of Testing index is realized, so when sample size is less, the consumption of sample can be saved；It can also control simultaneously
Sample preparation product enter the sequencing of reaction tank, so as to be controlled according to the difference reacted in reaction tank in different reactions
Between, to obtain optimal reaction result.
The utility model can adjust the electric heating sheets 6 of temperature by being provided with the underface of reaction tank, you can with root
According to the different controls reacted in reaction tank in different heating-up temperatures, the progress of reaction in reaction tank is not only contributed to, improves anti-
The efficiency of speed and detection is answered, and can be by controlling reaction tank to keep optimal reaction temperature to reach optimal reaction
As a result, the accuracy of reaction is improved, is conducive to the accurate analysis of multiple determination result.
In the present embodiment, electric heating sheets 6 can be connected with adjustable Constant Current Generator by wire, electricity is applied to by regulation
Voltage on heating plate 6 adjusts its heating power, so as to reach the purpose of regulation heating-up temperature.I.e. by controlling electric heating sheets
6, which are powered, produces joule's heat energy, and solution example in the microchannel of top is heated by heat transfer.Due to the electric heating sheets
6 are arranged on the bottom of base, i.e., it will not directly be contacted with fluid sample in microchannel, so as to avoid pollution and disturb.And
And the size of electric heating sheets is identical with reaction tank, therefore equably reaction tank can be heated, so as to be conducive to reaction
The progress reacted in pond, improves the accuracy of reaction speed and reaction, is conducive to the accurate analysis of multiple determination result.
Reaction tank 3 of the present utility model is 3, and 3~6 reaction tanks can be set according to the actual requirements, is not restricted to this
3 in embodiment.
The base 30 and upper lid 20 for the multiple determination micro-fluidic chip that the utility model is provided can select poly- diformazan
One kind in radical siloxane (PDMS), polymethyl methacrylate (PMMA), makrolon (PC).Base 30 and upper lid 20 can be with
It is bonded together by any mode in thermocompression bonding, laser bonding, ultrasonic wave added bonding, so as to realize the closed of chip
Sealing-in.
Principle of the present utility model is each composition structure formation 3- of upper lid and bottom seat when the parallel bonding of seat of upper lid and bottom
6 microchannels, sample fluid can flow in the presence of capillary force along microchannel, therefore fluid sample is added to sample
Product reception tank 1 reaches shunting pond 2 after Filter pad, enters to be measured contained by reaction tank 3, sample fluid after shunting
Antibody labeling thing in antigenic component and reaction tank 3 occurs immune response and is combined together, and then reaches and detects with sample fluid
The antibody of antigen-antibody-marker combination and detect tank 4 in groove 4, sample fluid containing band occurs immune response and stays in detection
Groove 4, remaining sample fluid flows through detecting element area and finally reaches waste liquid pool 5, stays in the immune response conjugate of detect tank 4
Amount, can be obtained by optical detection means detection, so as to realize the quantitative analysis to determined antigen.
It the above is only feature implementation example of the present utility model, the utility model protection domain be not limited in any way.
Technical scheme formed by all equal exchanges of use or equivalence replacement, all falls within the utility model rights protection scope.
Claims (8)
1. a kind of multiple determination micro-fluidic chip, including base (30) and upper lid (20), described base (30) include according to
Sample reception pond (1), shunting pond (2), several reaction tanks (3) and the waste liquid pool (5) of secondary connection, described reaction tank (3)
Connected by detect tank (4) with waste liquid pool (5)；Described upper lid (20) includes being arranged on position corresponding with sample reception pond (1)
The injection port (7) put and it is arranged on the driver plate (8) corresponding with shunting pond (2) position, it is characterised in that：Described driver plate
(8) rotating disk (81), handle (82) and import (83) and outlet (84) in rotating disk (81) circumference, upper lid (20) and base are included
(30) be bonded after, shunting pond (2) can be chimeric with driver plate (8), described import (83) is connected with sample reception pond (1), it is described go out
Mouth (84) is connected by rotating rotating disk (81) with reaction tank (3).
2. a kind of multiple determination micro-fluidic chip according to claim 1, it is characterised in that：The base (30)
Bottom is provided with adjusts the electric heating sheets (6) of heating-up temperature for being heated and can be needed according to reaction to reaction tank (3),
The quantity of described electric heating sheets (6) is equal with reaction tank (3) quantity, respectively positioned at the underface of reaction tank (3).
3. a kind of multiple determination micro-fluidic chip according to claim 2, it is characterised in that：Described electric heating sheets
(6) size is identical with reaction tank (3).
4. multiple determination micro-fluidic chip according to claim 1, it is characterised in that：Described upper lid (20) includes
It is arranged on the steam vent (9) of waste liquid pool (5) and sample reception pond (1) opposite position.
5. a kind of multiple determination micro-fluidic chip according to claim 1, it is characterised in that：The sample reception pond
(1) it is isosceles trapezoid, provided with fixed filter pad in sample reception pond (1), described filter pad is prepared for semipermeable membrane material, its
Shape and sample reception pond are adapted.
6. a kind of multiple determination micro-fluidic chip according to claim 1, it is characterised in that：Described reaction tank
(3) it is size identical ellipse, many indexes detection reagent is fixed in advance in reaction tank (3).
7. a kind of multiple determination micro-fluidic chip according to claim 1, it is characterised in that：The reaction tank (3)
For 3~6.
8. a kind of multiple determination micro-fluidic chip according to claim 1, it is characterised in that：Described detect tank
(4) it is size identical gallery, Multiple Antibodies is fixed with advance in detect tank (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720153206.7U CN206549664U (en) | 2017-02-20 | 2017-02-20 | A kind of multiple determination micro-fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720153206.7U CN206549664U (en) | 2017-02-20 | 2017-02-20 | A kind of multiple determination micro-fluidic chip |
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| Publication Number | Publication Date |
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| CN206549664U true CN206549664U (en) | 2017-10-13 |
Family
ID=60011742
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| Application Number | Title | Priority Date | Filing Date |
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| CN201720153206.7U Active CN206549664U (en) | 2017-02-20 | 2017-02-20 | A kind of multiple determination micro-fluidic chip |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107064216A (en) * | 2017-04-19 | 2017-08-18 | 哈尔滨工业大学 | A kind of portable COD detection means based on microfluidic chip technology |
| CN109882596A (en) * | 2017-12-06 | 2019-06-14 | 盾安环境技术有限公司 | A kind of pneumatic proportional valve based on MEMS micro-fluidic chip |
| CN110470600A (en) * | 2018-05-11 | 2019-11-19 | 中国石油化工股份有限公司 | Portable water quality many reference amounts detection device and detection method |
| CN113358883A (en) * | 2021-05-19 | 2021-09-07 | 扬州大学 | Micro-fluidic device for detecting plateau disease |
-
2017
- 2017-02-20 CN CN201720153206.7U patent/CN206549664U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107064216A (en) * | 2017-04-19 | 2017-08-18 | 哈尔滨工业大学 | A kind of portable COD detection means based on microfluidic chip technology |
| CN109882596A (en) * | 2017-12-06 | 2019-06-14 | 盾安环境技术有限公司 | A kind of pneumatic proportional valve based on MEMS micro-fluidic chip |
| CN110470600A (en) * | 2018-05-11 | 2019-11-19 | 中国石油化工股份有限公司 | Portable water quality many reference amounts detection device and detection method |
| CN110470600B (en) * | 2018-05-11 | 2024-02-27 | 中国石油化工股份有限公司 | Portable water quality multi-parameter detection equipment and detection method |
| CN113358883A (en) * | 2021-05-19 | 2021-09-07 | 扬州大学 | Micro-fluidic device for detecting plateau disease |
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