CN206538416U - One kind series connection fed-batch fermentation device - Google Patents
One kind series connection fed-batch fermentation device Download PDFInfo
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- CN206538416U CN206538416U CN201720098619.XU CN201720098619U CN206538416U CN 206538416 U CN206538416 U CN 206538416U CN 201720098619 U CN201720098619 U CN 201720098619U CN 206538416 U CN206538416 U CN 206538416U
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- series connection
- feed supplement
- batch fermentation
- fermentation device
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Abstract
The utility model is related to technical field of bioengineering, specifically related to a kind of series connection fed-batch fermentation device, including the fermentation tank being sequentially connected, feed supplement contact pin, connecting tube, feed supplementing device and filter, the feed supplementing device is provided with the peristaltic pump and feed supplement bottle that several are sequentially connected.A kind of series connection fed-batch fermentation device of the present utility model, the nutritive loss of different type replenisher mixing sterilizing can be avoided, it can realize that a variety of replenishers are added simultaneously again, material-feeding port on fermentation tank need not be increased, the number of operations of feed supplement contact pin is reduced, microbiological contamination hidden danger is reduced, ensure product quality, the consumption of filter is decreased, cost is reduced, it is ensured that fermentation efficiency.
Description
Technical field
The utility model is related to technical field of bioengineering, and in particular to one kind series connection fed-batch fermentation device.
Background technology
Microbial bacterial agent requires supplementation with some nutrient solutions, acid solution or alkali lye, chemical defoamer etc. during liquid fermentation,
In order to ensure product quality, these replenishers and pre- first pass through at high-temperature sterilization is all needed with the feed supplementing device that replenisher is directly contacted
Reason.It is general that feed supplement is carried out using feed supplement bottle plus peristaltic pump because required feed supplement amount is smaller for small-sized fermentation tank.It is conventional at present
Feeding medium during fermentation device be made up of feed supplement contact pin, feed supplement bottle, connecting tube, peristaltic pump and filter, in use, by these parts
It is put into together after connecting in autoclave sterilization pot and carries out sterilization treatment.After sterilizing, feed supplement contact pin is inserted into fermentation tank feed supplement
In mouthful, then connecting tube is connected on peristaltic pump and feed supplement bottle, feed supplement bottle connection filter.When needing feed supplement, start peristaltic pump,
Material in feed supplement bottle is added in fermentation tank, filter can ensure that feed supplement bottle is internal and be communicated with air, keep in feed supplement bottle
Portion's normal pressure, makes feed supplement more smooth.
Because often replenisher type is more in fermentation process, and many different types of replenishers can not be mixed
Sterilizing, can only use the bottled different replenisher of different feed supplements, conventional method is by each feed supplement equipped with different replenishers
Bottle all connects a feed supplement contact pin and filter, and feed supplement contact pin is inserted into material-feeding port, the material-feeding port so needed and mistake during feed supplement
Filter is more, troublesome poeration, and cost is high, also increases the number of operations of feed supplement contact pin, brings microbiological contamination hidden danger, influences product matter
Amount.
The content of the invention
The purpose of this utility model is, for of the prior art above-mentioned not enough there is provided one kind is easy to operate, can to mend simultaneously
A variety of replenishers are filled, microbiological contamination risk can be reduced, nutritive loss is reduced, reduces cost, efficient series connection fed-batch fermentation device.
The purpose of this utility model is achieved through the following technical solutions：
One kind series connection fed-batch fermentation device, including be sequentially connected fermentation tank, feed supplement contact pin, connecting tube, feed supplementing device and
Filter, the feed supplementing device is provided with the peristaltic pump and feed supplement bottle that several are sequentially connected.
Wherein, the fermentation tank is provided with agitating paddle, and agitating paddle both sides are respectively arranged with the first stirring frame and the second stirring
Frame.
Wherein, the first stirring frame is provided with some horizontally disposed horizontal grid of stirring.
Wherein, the second stirring frame is provided with some vertically disposed vertical grid of stirring.
Wherein, the connecting tube is insulating tube, and insulating tube is disposed with inner tube, heat-insulation layer and outer tube from the inside to the outside.
Wherein, the outer tube is provided with internal layer, stiff ribbon and outer layer from the inside to the outside.
Wherein, the filter is made up of upper conjunction box, lower conjunction box and filtration core, and upper conjunction box passes through screw thread and mistake with lower conjunction box
Filter core is connected.
Wherein, the filtration core includes active carbon layer, ceramic sandwich layer, nonwoven layer and sodium hydroxide layer.
The beneficial effects of the utility model：
First, a kind of series connection fed-batch fermentation device of the present utility model, can avoid the mixing sterilizing of different type replenisher
Nutritive loss, can realize that a variety of replenishers are added simultaneously again, without increasing material-feeding port on fermentation tank, reduce feed supplement contact pin
Number of operations, reduce microbiological contamination hidden danger, it is ensured that product quality, decrease the consumption of filter, reduce cost, it is ensured that fermentation effect
Rate.
2nd, the agitating paddle of the utility model fermentation tank is provided with the horizontal grid of stirring of laterally setting and longitudinally disposed stirring is indulged
Grid, stir horizontal grid during stirring and the vertical grid of stirring form different flow directions, make microorganism be difficult to be attached on agitating paddle, hinder stirring
Effect, is also easy to cleaning now.
3rd, the utility model connecting tube is provided with three-decker, and metastable temperature can be provided for replenisher, it is to avoid
Replenisher it is rotten.
4th, the utility model filter is provided with active carbon layer, ceramic sandwich layer, nonwoven layer and sodium hydroxide layer, multilayer
Filtration can make air in installation for fermenting keep certain cleanliness factor, and the balance of installation for fermenting and ambient pressure can be kept again,
Make feed supplement more smooth.
Brief description of the drawings
Utility model is described further using accompanying drawing, but the embodiment in accompanying drawing is not constituted to of the present utility model
What is limited, on the premise of not paying creative work, can also be according to the following drawings for one of ordinary skill in the art
Obtain other accompanying drawings.
Fig. 1 is a kind of structural representation for fed-batch fermentation device of connecting of the utility model.
Fig. 2 is a kind of cross-sectional view of the connecting tube for fed-batch fermentation device of connecting of the utility model.
Fig. 3 is a kind of decomposition texture schematic diagram of the filter for fed-batch fermentation device of connecting of the utility model.
Reference includes：1 fermentation tank, 2 feed supplement contact pins, 3 connecting tubes, 4 feed supplementing devices, 5 filters, 6 peristaltic pumps, 7 are stirred
Mix oar, the 8 horizontal grid of stirring, the vertical grid of 9 stirrings, 10 inner tubes, 11 heat-insulation layers, 12 outer tubes, close box, 14 time conjunction boxes, 15 filtration cores, 16 on 13
Active carbon layer, 17 ceramic sandwich layers, 18 nonwoven layers, 19 sodium hydroxide layers.
Embodiment
With the following Examples and accompanying drawing is further described to the utility model.
As shown in figure 1, a kind of series connection fed-batch fermentation device of the present utility model, including be sequentially connected fermentation tank 1, feed supplement
Contact pin 2, connecting tube 3, feed supplementing device 4 and filter 5, the feed supplementing device 4 are provided with the He of peristaltic pump 6 that several are sequentially connected
Feed supplement bottle.The one end of feed supplement contact pin 2 is the sharp groove of metal, and the other end is connected with connecting tube 3, and sharp bevel end can be inserted into fermentation
The material-feeding port of tank 1, connects fermentation tank 1.Feed supplement contact pin 2 is connected with feed supplement bottle by connecting tube 3, and peristaltic pump is connected in connecting tube 3
6.Feed supplementing device 4 at least containing more than two peristaltic pumps 6 and feed supplement bottle, peristaltic pump 6 and feed supplement bottle be sequentially connected, feed supplement bottle by
Material is drawn in body, bottle cap, long duct, tubulature composition, long duct insertion feed supplement bottom of bottle portion, and tubulature is deep into bottle liquid level
On, the other end of the connecting tube 3 connection feed supplement bottle long duct being connected with feed supplement contact pin 2, feed supplement bottle tubulature is connected by connecting tube 3
Connect another feed supplement bottle long duct, in peristaltic pump 6 indirectly, feed supplement bottle tubulature connection feed supplement bottle long duct, feed supplement bottle tubulature connects
Connect filter 5.
In use, by the feed supplement contact pin 2 connected, connecting tube 3, conduit, filter 5 and being respectively provided with different type feed supplement
The feed supplement bottle of liquid is put into high-pressure sterilizing pot simultaneously carries out sterilization treatment, after sterilizing cooling, opens peristaltic pump 6 by the thing of feed supplement bottle
Material is added in feed supplement bottle and mixed, and the material of feed supplement bottle then is added into feed supplement bottle is mixed, and material has been carried out in feed supplement bottle
Full mixing, is added in fermentation tank 1 finally by peristaltic pump 6, remixes feed supplement by being first sterilized separately processing, be avoided that together
Sterilizing causes feed supplement liquid to fail, and can realize that a variety of replenishers are added simultaneously again, without increasing material-feeding port on fermentation tank 1, reduces
The number of operations of feed supplement contact pin 2, reduces microbiological contamination hidden danger, it is ensured that product quality, decreases the consumption of filter 5, reduce into
This, it is ensured that fermentation efficiency.
The utility model fermentation tank 1 is provided with agitating paddle 7, and the both sides of agitating paddle 7 are respectively arranged with the first stirring frame and second
Stir frame.The first stirring frame is provided with some horizontally disposed horizontal grid 8 of stirring, and the second stirring frame is provided with some
The vertical grid 9 of the vertically disposed stirring of root.The both sides of agitating paddle 7 are the vertical grid 9 of asymmetrical stirring respectively and stir the horizontal structure of grid 8, are reduced
The aggregation of microorganism and tunning on grid, is also convenient for cleaning.Simultaneously different unsymmetric structure rotational bands is come irregular
Liquid flows, by increasing capacitance it is possible to increase the disturbance near zymotic fluid liquid level, so as to contribute to froth breaking, reduces the use of defoamer.
As depicted in figs. 1 and 2, the utility model connecting tube 3 is insulating tube, and insulating tube is disposed with inner tube from the inside to the outside
10th, heat-insulation layer 11 and outer tube 12, inner tube 10 are feed supplement liquid flow duct, and heat-insulation layer 11 can provide insulation for feed supplement liquid, it is to avoid feed supplement
Liquid reduces ferment effect due to temperature change, while outer tube 12 is protection pipe, with three-layer protection structure, can avoid touching
Hitting makes feed supplement liquid outflow, and also avoids polluting fermentation system, the outer tube 12 is provided with internal layer, stiff ribbon and outer layer from the inside to the outside.
As shown in figure 3, the utility model filter 5 is made up of upper conjunction box 13, lower conjunction box 14 and filtration core 15, upper conjunction box 13
It is connected with lower conjunction box 14 by screw thread with filtration core 15.Upper conjunction box 13 with lower conjunction box 14 to be symmetrical arranged, the two by screw thread and
Filtration core 15 is spirally connected, simple in construction, quick detachable, and the filtration core 15 includes active carbon layer 16, ceramic sandwich layer 17, nonwoven layer 18
With sodium hydroxide layer 19, active carbon layer 16 and ceramic sandwich layer 17 can adsorb bacterium, subparticle and the virus in air, nothing
The moisture content in air can be adsorbed by spinning layer of cloth 18 and sodium hydroxide layer 19, reduce the influence to feed supplement liquid.
Finally it should be noted that above example is only illustrating the technical solution of the utility model, rather than to this reality
With the limitation of novel protected scope, although being explained with reference to preferred embodiment to the utility model, this area it is general
Lead to it will be appreciated by the skilled person that can be modified to the technical solution of the utility model or equivalent substitution, without departing from this
The spirit and scope of utility model technical scheme.
Claims (8)
1. one kind series connection fed-batch fermentation device, it is characterised in that：Including the fermentation tank being sequentially connected, feed supplement contact pin, connecting tube, benefit
Expect device and filter, the feed supplementing device is provided with the peristaltic pump and feed supplement bottle that several are sequentially connected.
2. a kind of series connection fed-batch fermentation device according to claim 1, it is characterised in that：The fermentation tank is provided with stirring
Oar, agitating paddle both sides are respectively arranged with the first stirring frame and the second stirring frame.
3. a kind of series connection fed-batch fermentation device according to claim 2, it is characterised in that：The first stirring frame is provided with
Some horizontally disposed horizontal grid of stirring.
4. a kind of series connection fed-batch fermentation device according to claim 2, it is characterised in that：The second stirring frame is provided with
Some vertically disposed vertical grid of stirring.
5. a kind of series connection fed-batch fermentation device according to claim 1, it is characterised in that：The connecting tube is insulating tube,
Insulating tube is disposed with inner tube, heat-insulation layer and outer tube from the inside to the outside.
6. a kind of series connection fed-batch fermentation device according to claim 5, it is characterised in that：The outer tube is set from the inside to the outside
There are internal layer, stiff ribbon and outer layer.
7. a kind of series connection fed-batch fermentation device according to claim 1, it is characterised in that：The filter by it is upper conjunction box,
Lower conjunction box and filtration core composition, upper conjunction box is connected with lower conjunction box by screw thread with filtration core.
8. a kind of series connection fed-batch fermentation device according to claim 7, it is characterised in that：The filtration core includes activated carbon
Layer, ceramic sandwich layer, nonwoven layer and sodium hydroxide layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720098619.XU CN206538416U (en) | 2017-01-26 | 2017-01-26 | One kind series connection fed-batch fermentation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720098619.XU CN206538416U (en) | 2017-01-26 | 2017-01-26 | One kind series connection fed-batch fermentation device |
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| Publication Number | Publication Date |
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| CN206538416U true CN206538416U (en) | 2017-10-03 |
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| CN201720098619.XU Active CN206538416U (en) | 2017-01-26 | 2017-01-26 | One kind series connection fed-batch fermentation device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112500997A (en) * | 2020-12-18 | 2021-03-16 | 上海钧济医学检验所有限公司 | Auxiliary device for online culture solution supplementation of antibacterial drug in-vitro PK/PD model and use method of auxiliary device |
-
2017
- 2017-01-26 CN CN201720098619.XU patent/CN206538416U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112500997A (en) * | 2020-12-18 | 2021-03-16 | 上海钧济医学检验所有限公司 | Auxiliary device for online culture solution supplementation of antibacterial drug in-vitro PK/PD model and use method of auxiliary device |
| CN112500997B (en) * | 2020-12-18 | 2023-08-04 | 上海原科实业发展有限公司 | Auxiliary device for online culture fluid supplementation of antibacterial drug in-vitro PK/PD model and use method thereof |
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