CN206473315U - A kind of blood flow three-dimensional image forming apparatus based on lamella light - Google Patents
A kind of blood flow three-dimensional image forming apparatus based on lamella light Download PDFInfo
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- CN206473315U CN206473315U CN201621192297.7U CN201621192297U CN206473315U CN 206473315 U CN206473315 U CN 206473315U CN 201621192297 U CN201621192297 U CN 201621192297U CN 206473315 U CN206473315 U CN 206473315U
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Abstract
The utility model is related to a kind of blood flow three-dimensional image forming apparatus based on lamella light, and the device includes laser, beam splitter, cylindrical mirror, drive device, imaging device, triggering device and computer；Beam splitter is split to the laser that laser is launched, and cylindrical mirror is by the laser formation lamella light irradiation after beam splitting on sample；Imaging device and triggering device are connected with computer；Computer controls drive device to drive sample placed on it to do lengthwise movement by triggering device；Imaging device is used for the view data for gathering sample and by described image data transfer to computer, and the view data that computer docking is received is handled and sets up the three-dimensional flow image of sample.A kind of blood flow three-dimensional image forming apparatus based on lamella light that the utility model is provided, by controlling sample lengthwise movement so that each layer of sample is sequentially located at the irradiation position of lamella light, imaging device gathers view data and transmits it to computer and handled to set up the three-dimensional flow image of sample in each layer of sample, and imaging precision is high.
Description
Technical field
The utility model is related to biological tissue's blood flow imaging technical field, more particularly, to a kind of based on lamella light
Blood flow three-dimensional image forming apparatus.
Background technology
Embryonic development is the important topic in life science field, in embryo development procedure the formation of histoorgan with
Growth is required to blood and provides oxygen and nutrient.Development it is a kind of can be used for observation embryo development procedure medium vessels network distribution into
As there is highly important scientific research and application value with monitoring method.And with the development of clinical medicine and experimental study, blood
No matter flow imaging has great value in terms of clinical medicine application or scientific research.At present, optics blood flow imaging side
Method mainly has laser speckle blood current imaging, and this method is dissipated by gathering N frames with identical time for exposure and frame period Time Continuous
Spot image；The pixel in same position correspondence N × N size spatial neighborhoods in each two field picture is taken out, set of pixels is constituted, calculates the neighbour
Contrast in domain, then contrast so that each pixel is corresponding for gray scale, build blood-stream image.
Existing laser speckle blood current imaging method is broadly divided into following three kinds：Contrast imaging method in space：For example China is real
" side of microcirculation blood flow spatio-temporal response characteristic on laser speckle imager detection intestines cell membrane is utilized with new patent CN1391869A
Method ", the major defect of this method is that image spatial resolution is relatively low.Time contrasts imaging method：Such as Chinese utility model patent
CN1792323A " a kind of transcranial cerebral blood flow high-resolution rate imaging method and its device ", the major defect of this method is image temporal
Resolution ratio is relatively low, is easily influenceed by flating.Space-time, which is combined, contrasts imaging method：Chinese utility model patent
CN101485565A " a kind of laser speckle blood current imaging and analyzing method ", Chinese utility model patent CN102429650A are " a kind of
Laser speckle blood flow imaging contrast analytical method ", both approaches are all the method raising imagings that utilization space and time combine
Spatial resolution and temporal resolution, but all have that algorithm operation quantity is excessive, slow problem.In addition, laser dissipates
Spot blood flow imaging all haves the shortcomings that three-dimensional flow imaging can not be carried out.
In addition, the method for existing three-dimensional flow imaging also has Laser Scanning Confocal Microscope and micro- nuclear magnetic resonance technique.Copolymerization
The shortcoming of burnt microtechnic is can not to carry out non-fluorescence imaging, and can not carry out three-dimensional imaging to complete embryo.Micro- nuclear-magnetism is total to
The shortcoming for technology of shaking is that image resolution ratio is low, and expensive.
Utility model content
The purpose of this utility model be to overcome the deficiencies in the prior art there is provided a kind of blood flow based on lamella light it is three-dimensional into
As device, the installation cost is cheap, and sample nondestructive is done harm to, and can realize lamella light demixing scan, and can obtain three-dimensional flow image,
And imaging precision is high.
In order to solve the above technical problems, the technical solution adopted in the utility model is：
A kind of blood flow three-dimensional image forming apparatus based on lamella light, including laser, beam splitter, cylindrical mirror, driving dress are provided
Put, imaging device, triggering device and computer；The beam splitter is split to the laser that laser is launched, and cylindrical mirror will divide
Laser formation lamella light irradiation after beam is on sample；Imaging device and triggering device are connected with computer；Computer passes through triggering
Device control drive device drives sample placed on it to do lengthwise movement；Imaging device is for gathering the view data of sample simultaneously
By described image data transfer to computer, the view data that computer docking is received is handled and sets up the three-dimensional flow figure of sample
Picture.
In such scheme, by setting cylindrical mirror, drive device, imaging device, triggering device and computer so that computer expert
Triggering device control drive device driving sample is crossed when doing lengthwise movement, each layer of sample be sequentially located at cylindrical anamorph into piece
At the irradiation position of layer light, imaging device gathers view data and transmits it to computer and handled in each layer of sample
To set up the three-dimensional flow image of sample.A kind of blood flow three-dimensional image forming apparatus based on lamella light of the utility model, it is with low cost,
Sample nondestructive is done harm to, lamella light demixing scan can be realized, and three-dimensional flow image can be obtained, and imaging precision is high.
Preferably, the imaging device is COMS cameras, and the camera lens of the COMS cameras is long-focus telecentric lens.So
Setting can not only obtain the view data of more high spatial resolution, and can save cost.
Preferably, the output end of drive device is connected with platform, and the platform is used to fix sample.Drive device work makes
Platform does lengthwise movement, and platform motion drives sample motion, make sample each layer be sequentially located at cylindrical anamorph into lamella light
Irradiation position at.The fixation for being easy to sample is so set, prevents that sample is rocked when drive device from working, the image shot is influenceed
The quality of data.
Preferably, the drive device is stepper motor.
Preferably, the laser that laser is launched is divided into four beams by the beam splitter, and cylindrical mirror is four.Cylindrical mirror is put
The surrounding in sample is put, and the lamella light for making four cylindrical mirrors be at identical height, such four positions is highly consistent, from
And make it that the irradiation light intensity of sample is more uniform has the consistent depth of field so that the view data effect of imaging device collection is more
It is good, be conducive to improving the precision for the three-dimensional flow image set up.
Compared with prior art, the beneficial effects of the utility model are：
A kind of blood flow three-dimensional image forming apparatus based on lamella light of the utility model, by set cylindrical mirror, drive device, into
As device, triggering device and computer so that when computer does lengthwise movement by triggering device control drive device driving sample, sample
Each layer of product be sequentially located at cylindrical anamorph into lamella light irradiation position at, imaging device is gathered in each layer of sample
View data simultaneously transmits it to computer and handled to set up the three-dimensional flow image of sample, and the installation cost is cheap, to sample
Product are harmless, can realize lamella light demixing scan, and can obtain three-dimensional flow image, and imaging precision is high；By the way that imaging is filled
Install and be set to COMS cameras, the camera lens of COMS cameras is set to long-focus telecentric lens, can not only obtain more high-space resolution
The view data of rate, and cost can be saved；By the way that the laser that beam splitter is arranged to launch in laser is divided into four beams, post
Face mirror is set to four, laser is pointed into sample from surrounding by cylindrical mirror so that the irradiation light intensity of sample is more uniform to be had
The consistent depth of field so that the view data effect of imaging device collection more preferably, is conducive to improving the three-dimensional flow image of foundation
Precision；Using the whole audience blood flow imaging of noncontact optical measurement, without injecting contrast agent, sample nondestructive is done harm to, lamella can be realized
Light demixing scan, three-dimensional flow image, and imaging precision can be set up on the basis of every layer of two-dimensional projection's blood-stream image of acquisition
It is high.
Brief description of the drawings
Fig. 1 is a kind of structural representation of the blood flow three-dimensional image forming apparatus based on lamella light of the present embodiment.
Fig. 2 is Fig. 1 top view, wherein not comprising imaging device, computer and triggering device.
Fig. 3 is that sample simulates layering and irradiation position movement schematic diagram, wherein dotted line and the solid line blood that to represent two different
Pipe.
Two-dimensional projection's blood-stream image that Fig. 4 gathers for z layers of the sample of simulation.
Two-dimensional projection's blood-stream image that Fig. 5 gathers for z-1 layers of the sample of simulation.
Fig. 6 is the real blood-stream image of z layers of sample.
Fig. 7 is the flow chart of image data acquiring.
Fig. 8 is the flow chart for the three-dimensional flow image that computer handles image data acquisition sample.
Embodiment
The utility model is further described with reference to embodiment.Wherein, being given for example only property of accompanying drawing
Illustrate, expression is only schematic diagram, rather than pictorial diagram, it is impossible to be interpreted as the limitation to this patent；In order to which this reality is better described
With new embodiment, some parts of accompanying drawing have omission, zoomed in or out, and do not represent the size of actual product；To ability
For field technique personnel, some known features and its explanation may be omitted and will be understood by accompanying drawing.
The same or analogous part of same or analogous label correspondence in the accompanying drawing of the utility model embodiment；In this practicality
In new description, it is to be understood that if the orientation or position relationship that have the instructions such as term " on ", " under ", "left", "right" are
Based on orientation shown in the drawings or position relationship, it is for only for ease of description the utility model and simplifies description, rather than indicate
Or imply that signified device or element must have specific orientation, to be retouched in specific azimuth configuration and operation, therefore accompanying drawing
Term the being given for example only property explanation of position relationship is stated, it is impossible to be interpreted as the limitation to this patent, for the common skill of this area
For art personnel, the concrete meaning of above-mentioned term can be understood as the case may be.
Embodiment
A kind of schematic diagram of the blood flow three-dimensional image forming apparatus based on lamella light of the present embodiment as depicted in figs. 1 and 2, including swashs
Light device 1, beam splitter 2, cylindrical mirror 3, drive device 4, imaging device 6, triggering device 8 and computer 9；The beam splitter 2 is to laser
The laser that device 1 is launched is split, and cylindrical mirror 3 is by the laser formation lamella light irradiation after beam splitting on sample 5；Imaging device
6 and triggering device 8 be connected with computer 9；Computer 9 controls drive device 4 to drive sample 5 placed on it by triggering device 8
Do lengthwise movement；Imaging device 6 is used to gather the view data of sample 5 and by described image data transfer to computer 9, computer 9
View data to reception is handled and sets up the three-dimensional flow image of sample 5.
In the present embodiment, computer 9 controls drive device 4 to drive sample 5 placed on it to do from up to by triggering device 8
Under lengthwise movement.
During using the device, user sends signal, the control drive device of triggering device 8 by computer 9 to triggering device 8
4 driving samples 5 do lengthwise movement from top to bottom, each layer of sample 5 is sequentially located at the photograph for the lamella light that cylindrical mirror 3 is formed
Penetrate at position, imaging device 6 gathers view data and transmit it to computer 9 and handled to build in each layer of sample 5
The three-dimensional flow image of vertical sample 5.A kind of blood flow three-dimensional image forming apparatus based on lamella light of the utility model, it is with low cost, it is right
Sample nondestructive does harm to, and can realize lamella light demixing scan, and can obtain three-dimensional flow image, and imaging precision is high.
Wherein, the imaging device 6 is COMS cameras, and the camera lens of the COMS cameras is long-focus telecentric lens 7.So
Setting can not only obtain the view data of more high spatial resolution, and can save cost.
In addition, the output end of drive device 4 is connected with platform 10, the platform 10 is used to fix sample 5.Drive device 4
Work the lengthwise movement for making platform 10 do from top to bottom, and the motion of platform 10 drives sample 5 to move from top to bottom, makes the every of sample 5
One layer is sequentially located at the irradiation position of the lamella light of the formation of cylindrical mirror 3.The fixation for being easy to sample 5 is so set, driving is prevented
Sample 5 is rocked when device 4 works, and influences the quality of view data shot.
Wherein, the drive device 4 is stepper motor.
In addition, the laser that laser 1 is launched is divided into four beams by the beam splitter 2, cylindrical mirror 3 is four, such as Fig. 2 institutes
Show.Cylindrical mirror 3 is placed on to the surrounding of sample 5, and four cylindrical mirrors 3 is at identical height, such four positions
Lamella light is highly consistent, so that the irradiation light intensity of sample 5 is more uniform the consistent depth of field so that imaging device 6 is adopted
The view data effect of collection more preferably, is conducive to improving the precision for the three-dimensional flow image set up.
The blood flow three-dimensional image forming apparatus based on lamella light provided using the utility model is to the blood flow three based on lamella light
Dimension imaging, comprises the following steps：
S1. the view data of sample 5 is gathered：Sample 5 is placed on to the top of drive device 4, computer 9 passes through triggering device
8 control drive devices 4 drive sample 5 placed on it to do lengthwise movement from top to bottom so that the first layer of sample 5 to n-th
Layer is sequentially located at lamella light irradiation position, and imaging device 6 gathers m images, every IMAQ in each layer of sample 5
Speed is f；The collecting flowchart figure of view data is as shown in fig. 7, mobile example 5 causes lamella light irradiation the 1st of sample first
At layer, gathered every the 1/f times after an image, m images of collection, move down sample 5 so that lamella light irradiation is in sample
2nd layer, then gather m images again in a manner described, and so on, until having gathered m images of n-th layer, stop collection,
Fig. 3 is that sample 5 simulates layering and irradiation position movement schematic diagram；
S2. imaging device 6 transmits the view data of the sample 5 collected to computer 9, the picture number of 9 pairs of receptions of computer
According to the three-dimensional flow image for being handled and being set up sample 5, process chart is as shown in figure 8, comprise the following steps：
S21. Fast Fourier Transform (FFT) of the pixel progress along time shaft one by one of the m collected to each layer images, with
Obtain the spectrum value of time shaft of each pixel along m images：
Iz(x,y,t)→Iz(x,y,u)(1)
Wherein, Iz (x, y, t) represents z layers of pixel (x, y) along the signal light intensity at time shaft t, and Iz (x, y, u) is represented
Z layers of pixel (x, y) frequency domain intermediate frequency spectrum, z is less than or equal to n；
S22. frequency domain filtering is carried out, dynamic signal data and stationary singnal data is extracted respectively, and by Dynamic Signal number
According to sum and stationary singnal data sum ratio as each pixel of every tomographic image imaging parameter：
Wherein, IRBC(x, y, z) is the imaging parameter of z layers of pixel (x, y) place reconstruction image, id(x, y, u) is z
The dynamic signal data that layer pixel (x, y) place correspondence collection signal is extracted；is(x, y, 0) is z layers of pixel (x, y) place
The stationary singnal data that correspondence collection signal is extracted, μ 0=10Hz, f are every IMAQ speed；
S23. the view data of the sample gathered is the upward lateral scattering optical signal of sample, therefore the view data of collection
Contain all blood flow informations on the position and the position；The imaging parameter of each pixel of every tomographic image is subtracted into last layer
The imaging parameter of image corresponding pixel points, to obtain the corresponding real imaging parameter of each pixel in every tomographic image：
I′RBC(x, y, z)=IRBC(x,y,z)-IRBC(x, y, z-1) (3) wherein, I 'RBC(x, y, z) is every layer of projection blood flow
The corresponding real imaging parameter of each pixel in image；It is assumed that two-dimensional projection rheographies of the Fig. 4 for the z layers of collection of sample of simulation
Picture, it is assumed that two-dimensional projection's blood-stream image that Fig. 5 gathers for z-1 layers of the sample of simulation, then the two dimension throwing of z layers of collection of sample
Shadow blood-stream image subtracts the real blood-stream image of z layers of two-dimensional projection's blood-stream image derived sample of z-1 layers of collection of sample, such as
Shown in Fig. 6；
S24. sample (5) each layer will be set up as gray value by corresponding real imaging parameter per each pixel of tomographic image
Two-dimensional projection's blood-stream image, the three-dimensional flow image of sample (5) is set up using n-layer two-dimensional projection blood-stream image.
The whole audience blood flow imaging of this noncontact optical measurement, without injecting contrast agent, does harm to sample nondestructive, can realize piece
Layer light demixing scan, can set up three-dimensional flow image, and imaging essence on the basis of every layer of two-dimensional projection's blood-stream image of acquisition
Degree is high.
Wherein, the sample 5 is the embryo of chicken or fish.
Obviously, above-described embodiment of the present utility model is only intended to clearly illustrate the utility model example, and
It is not the restriction to embodiment of the present utility model.For those of ordinary skill in the field, in described above
On the basis of can also make other changes in different forms.There is no need and unable to give all embodiments
It is exhaustive.All any modifications, equivalent substitutions and improvements made within spirit of the present utility model and principle etc., should be included in
Within the utility model scope of the claims.
Claims (5)
1. a kind of blood flow three-dimensional image forming apparatus based on lamella light, it is characterised in that including laser (1), beam splitter (2), post
Face mirror (3), drive device (4), imaging device (6), triggering device (8) and computer (9)；The beam splitter (2) is to laser (1)
The laser launched is split, and cylindrical mirror (3) is by the laser formation lamella light irradiation after beam splitting on sample (5)；Imaging dress
(6) and triggering device (8) is put to be connected with computer (9)；Computer (9) controls drive device (4) driving to put by triggering device (8)
Sample (5) thereon does lengthwise movement；Imaging device (6) is used to gather the view data of sample (5) and by described image number
According to transmitting to computer (9), computer (9) is handled the view data of reception and sets up the three-dimensional flow image of sample (5).
2. a kind of blood flow three-dimensional image forming apparatus based on lamella light according to claim 1, it is characterised in that the imaging
Device (6) is COMS cameras, and the camera lens of the COMS cameras is long-focus telecentric lens (7).
3. a kind of blood flow three-dimensional image forming apparatus based on lamella light according to claim 1, it is characterised in that drive device
(4) output end is connected with platform (10), and the platform (10) is used to fix sample (5).
4. a kind of blood flow three-dimensional image forming apparatus based on lamella light according to claim 1, it is characterised in that the driving
Device (4) is stepper motor.
5. a kind of blood flow three-dimensional image forming apparatus based on lamella light according to any one of Claims 1-4, its feature exists
In the laser that laser (1) is launched is divided into four beams by the beam splitter (2), and cylindrical mirror (3) is four.
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| CN201621192297.7U CN206473315U (en) | 2016-10-31 | 2016-10-31 | A kind of blood flow three-dimensional image forming apparatus based on lamella light |
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| CN201621192297.7U CN206473315U (en) | 2016-10-31 | 2016-10-31 | A kind of blood flow three-dimensional image forming apparatus based on lamella light |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106419889A (en) * | 2016-10-31 | 2017-02-22 | 佛山科学技术学院 | Device and method for three-dimensionally imaging blood flow based on lamella light |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106419889A (en) * | 2016-10-31 | 2017-02-22 | 佛山科学技术学院 | Device and method for three-dimensionally imaging blood flow based on lamella light |
| CN106419889B (en) * | 2016-10-31 | 2023-12-05 | 佛山科学技术学院 | Three-dimensional blood flow imaging device and method based on lamellar light |
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