CN206428254U - Digital PCR biochip sample adding device - Google Patents

Abstract

The utility model discloses a kind of digital pcr biochip sample adding device, including fixed plummer, the pedestal of sample-adding brush and chip lid, heating module, sample-adding brush is removably secured sample charging mechanism thereon, it is fixedly linked with sample charging mechanism, slow down the slow descending mechanism of the sinking speed of sample-adding brush, the auxiliary descending mechanism contacted with each other after being fixedly linked with sample charging mechanism by secondary landing with chip, control panel, and the matched moulds arm being hinged and connected provided with perforate power switch and outlet by compressing switch with the shell of diffuser and by rotating shaft and pedestal, matched moulds arm is provided with the retaining mechanism of fixed chip lid；Chip lid is removably attached on retaining mechanism；Matched moulds arm rotates to plummer around the shaft covers chip lid on chip.The technical program enables digital pcr aqueous mixture to be uniformly loaded into chip, with sample-adding precision it is high, be loaded that uniform, reproducible, sample-adding is rapid, automaticity is high, operate easily advantage.

Description

Digital pcr biochip sample adding device

Technical field

The utility model is related to biochip test field, more particularly to digital pcr biochip sample adding device.

Background technology

Digital pcr is Digital PCR (dPCR), and it is a kind of nucleic acid molecules absolute quantitation technology.Compared to qPCR, number Word PCR can allow you directly to count the number of DNA molecular, be the absolute quantitation to initial sample.Quantitative PCR is to rely on standard Curve or reference gene determine nucleic acid amount, and digital pcr then allows you directly to count the number of DNA molecular, be to starting The absolute quantitation of sample.Suitable for the application field that can not be differentiated very well by Ct values：Such as copy number variation, abrupt climatic change, It is gene relative expression research (such as allele imbalance expression), the checking of two generation sequencing results, miRNA expression analysis, unicellular Gene expression analysis etc..

There are the following problems in additional steps for existing digital pcr biochip：1st, the aqueous phase being added on chip is mixed Compound is influenceed larger by variation of ambient temperature；2nd, existing sample loading alternative automaticity is relatively low, and sample-adding precision is not accurate enough.

Utility model content

In order to overcome the deficiencies in the prior art, the utility model provide one kind temperature can be maintained under steady state and The higher digital pcr biochip sample adding device of automaticity.

To achieve the above object, the technical solution adopted in the utility model is：A kind of digital pcr biochip sample-adding dress Put, include the pedestal of fixed plummer, sample-adding brush and chip lid, plummer is provided with the locating slot of chip placement；In locating slot Provided with the equally distributed projection for holding up chip；Also include heating module：Thermal source is provided chip and by temperature stabilization in rule Determine in scope, heating module is fixedly installed on one end of pedestal, plummer is detachably secured to the top of heating module；Sample-adding machine Structure：It is fixed on pedestal, sample-adding brush is removably fixedly linked with sample charging mechanism, sample-adding brush edge first under sample charging mechanism drive It is slow after being moved straightly to close to above plummer to drop to directly over chip；Slow descending mechanism：It is fixedly linked, subtracts with sample charging mechanism The sinking speed of slow sample-adding brush；Auxiliary descending mechanism：Be fixedly linked with sample charging mechanism, after the secondary landing of auxiliary sample-adding brush with locating slot Chip be in contact；Control panel：Control is provided to device, control panel is electrically connected with heating module and sample charging mechanism；Shell：With base Sample charging mechanism, slow descending mechanism and auxiliary descending mechanism are covered on and protected in it by seat after being fixedly linked；Shell is corresponding with sample charging mechanism Place is provided with the perforate for facilitating sample charging mechanism to advance or retreat；The side of shell is provided with power switch and outlet；Shell Top is provided with by compressing switch and diffuser；The surface that diffuser is arranged on the LED being fixed on control panel appears light；Electricity Source switch, outlet, electrically connected respectively with control panel by compressing switch；Matched moulds arm：It is hinged and connected by rotating shaft with pedestal；Matched moulds Arm is provided with the retaining mechanism of fixed chip lid；Chip lid is removably attached on retaining mechanism；Matched moulds arm rotates around the shaft Chip lid is covered on chip to plummer.

After the technical solution of the utility model：Device is connected with extraneous power supply by outlet, turned on the power Switch is powered to whole device, and heating module is heated automatically after start, is waited and is reached after design temperature, passes through LED The change of lamp color reminds the user can to start working.Chip is placed into locating slot and chip is lifted by projection, Sample-adding brush is fixed on sample charging mechanism, in the retaining mechanism that chip lid is fixed on to matched moulds arm.14.5uL is pipetted with liquid-transfering gun Reactant mixture, it is hanging to be added dropwise in sample-adding brush middle concave groove portion, pressed after completion by compressing switch, control panel output refers to Make to sample charging mechanism, sample charging mechanism drives direction of the sample-adding brush first towards chip to move horizontally, and waits and closes on directly over chip When, sample charging mechanism is realized to fall for the first time in the cooperation of slow descending mechanism, shortens the vertical range between sample-adding brush and chip.Then By second of whereabouts of auxiliary descending mechanism, chip brush is just realized with chip to be contacted with each other, and now sample charging mechanism drives sample-adding brush Motion, the reagent for being loaded the face that paints uniformly is brushed on chip, is covered in after having brushed with oil phase after chip aperture.By matched moulds arm Rotate around the shaft to the top of plummer and cover chip lid on chip, continuing to refuel in the hole that past chip is covered carries out oil sealing, Finally clung again with fixing glue, you can make a complete biochip.

This product is an instrument of sample introduction in digital pcr system, is uniformly painted on by being analysed to sample on chip, Combination PCR amplification instrument is expanded, and signal analysis is carried out on biological chip reading apparatus, you can to the target molecules number in sample Amount carries out absolute counting.It is therefore particularly suitable for human genome project is learned, legal medical expert's tumour is knitted with Population Biology, botany etc. Research field and virus, tumour, the quantitative detection in the clinical diagnosis field of hereditary disease.

The beneficial effect brought using the technical program：1st, the encapsulation of biochip is maintained into stationary temperature, kept away Influence of the change to testing result of temperature data is exempted from.2nd, sample-adding process is realized by way of automation, is improved and is added The automaticity of sample process.3rd, operating noise≤20db of whole device；Scraping blade speed 50S/ pieces；Sample introduction rate >=90%；Temperature Spend precision≤0.3 DEG C.By above-mentioned data, it is apparent that noise is low when the utility model has work, scraping blade speed is fast, The advantages of sample introduction rate height and accurate temperature control, the accuracy to digital PCR system Monitoring Data provides favourable guarantee.

As a further improvement, sample charging mechanism includes adjutage, connecting seat, fixed seat, rolling bearing and spindle motor； Adjutage one end is fixedly linked with connecting seat, and one end and the fixed seat of fixed sample-adding brush are fixedly linked；Fixed seat is provided with convenient Change the Anchor plate kit one of sample-adding brush；Boss of the bottom surface of adjutage provided with one section of inverted trapezoidal；Two sides of boss to extension Inclined plane is provided between the bottom surface of arm；Rolling bearing is fixed on pedestal, and the bottom surface face of original state rolling bearing and boss connects Touch, as the mobile rolling bearing of adjutage excessively arrives the bottom plane-plane contact with adjutage by means of inclined plane；Lead screw motor is consolidated It is scheduled in pedestal, lead screw motor is electrically connected with control panel, spindle motor is fixedly linked with connecting seat；The bottom of connecting seat be provided with The rail groove that the line slideway being fixed in pedestal cooperates；Connecting seat is under the drive of spindle motor relative to line slideway Advance or retreat with the screw mandrel of spindle motor.

After this programme, under original state, rolling bearing is in the lower section of boss, by pressing switch triggering lead screw motor Work starts, and lead screw motor drives connecting seat to be run forward along screw mandrel and line slideway, due to the bottom of rolling bearing and boss Face carries out face contact, and now adjutage is moved forward along horizontal direction, with working on for lead screw motor, by means of tilting Face rolling bearing can carry out face with the bottom surface of the sample arm on right side at leisure and contact, and then realize that the first time of sample-adding brush lands, Shorten the vertical range between sample-adding brush and chip.

As a further improvement, fixed seat includes contiguous block one and contiguous block two；Contiguous block one and adjutage stationary phase Even；Mutually corresponding two side of contiguous block two is provided with chucking lug；Contiguous block two by after chucking lug and the clamping of contiguous block one again It is fixedly linked；Contiguous block two is provided with projection；The projection corresponding position of sample-adding brush and contiguous block two is provided with locating slot；In sample-adding brush Between inclined buffer table is provided with groove.

After the technical program, after chucking lug ensure that contiguous block two is fixed with connecting plate, it will not be sent out because of error Raw skew, passes through projection and is cooperated with locating slot, it is ensured that after sample-adding brush is fixed will not because of by external force with being connected Relative displacement influence work occurs for block two.Because during sample-adding, liquid-transfering gun can not prop up the inner surface sample-adding of brush, pass through The buffering of buffer table, pipettor will not prop up the inner surface of brush in whole liquid feeding process, while improving sample-adding efficiency Also ensure that will not have influence on final measuring accuracy because of the incorrect of liquid feeding process.

As a further improvement, the Anchor plate kit one includes pressing plate one, steady pin and torsion spring；Pressing plate one is provided with solid The block tongue of fixed sample-adding brush；Sample-adding is painted provided with neck corresponding with block tongue；Contiguous block two is provided with fixed ear；Steady pin and fixation Ear is fixedly linked；Pressing plate one is hinged and connected with contiguous block two by steady pin；Torsion spring is fastened on steady pin, and the one of torsion spring End is connected in the torsional spring slot of pressing plate one, and the other end is connected at block tongue；Pressing plate one is solid by sample-adding brush locking under torsion spring effect It is fixed.

After the technical program, by press pressing plate one can be achieved between sample-adding brush and fixed seat it is quick fixed with Separation, whole Renewal process is simple and facilitates.

As a further improvement, the screw mandrel of lead screw motor is provided with screw rodb base；Screw rodb base is made up of elastomeric material Fixed column is fixedly linked with connecting seat；Fixed column is symmetricly set on the both sides of screw mandrel.

After the technical program, by conventional being rigidly connected, this is flexible connection between screw mandrel and connecting seat, even if dress The deviation occurred once in a while with process can also obtain self during sample arm is movable, solve and be rigidly connected The problem of screw mandrel and easily stuck connecting seat.

As a further improvement, optoelectronic switch and monitoring matched moulds arm of the control panel provided with control adjutage extended distance The optical coupled switch of rotation；The baffle plate that connecting seat starts provided with triggering optoelectronic switch.

After the technical program, optoelectronic switch realizes the control to sample arm shift position, passes through regulating fender Relative position, sample-adding brush, which can be realized, accurately to be fallen on chip down.The use of optical coupled switch, when optical coupled switch detects matched moulds After arm is passed through from top, can there are the signal of a jerk, the problem of efficiently solving maloperation to lead screw motor.

As a further improvement, slow descending mechanism includes damper and damping spring；Damper is fixedly linked with adjutage； Damper is provided with equally distributed fixing hole around central shaft；Damping spring is wrapped on damper；One end of damping spring and company Joint chair is fixedly linked, and the other end is connected in the fixing hole of damper.

It is big to damper resistance to realize in different fixing holes by adjusting damping spring after the technical program Small adjustment.

As a further improvement, auxiliary descending mechanism includes the plunger that guide holder, connecting rod and elastomeric material are made；On guide holder Provided with rail groove and behaviour area；The length of behaviour area is less than the length of boss；Rail groove adds the length of behaviour area to be more than boss Length；The height of rail groove is more than the height of connecting rod；One end of connecting rod is hinged with adjutage to be fixedly linked, and the other end is provided with guiding Bar, original state guide rod is arranged on behaviour area；Plunger at one end is fixedly linked with connecting rod, and the other end is fixedly linked with adjutage； Original state plunger is in compressive state.

After the technical program, before first time declines, rolling bearing is raised adjutage by boss, delays drop machine Structure is with respect to the fulcrum between adjutage and connecting rod, according to lever principle, and now connecting rod has downward power, due to connecting rod and extension Plunger is provided between arm, when now plunger is in the compression stage, i.e. original state, plunger is at compressive state, by Length in behaviour area is less than the length of boss, and as adjutage moves forward, the guide rod of connecting rod can enter track from behaviour area In groove.The length of other rail groove adds the length of behaviour area to be more than the length of rail groove again, declines for the first time after completing, is oriented to Bar is also in guide groove；Adjutage continued advancement at any time, guide rod out, receives the work of plunger reaction force out of rail groove With connecting rod has an action shifted up, and now adjutage has the action of a whereabouts, is made by slow descending mechanism With this lower backward sample-adding brush reaches exactly to chip and contacted with each other in chip.Complete after sample-adding, lead screw motor is inverted, band Dynamic connecting seat is successively mobile, and during rear shifting, guide rod is streaked at the top of rail groove when adjutage is lifted by boss Rise after, connecting rod is moved down ram compression is deformed after, guide rod is turned again in behaviour area.

As a further improvement, adjutage is provided with spacing block set, and connecting rod is under the cooperation of guide holder and spacing block set Rotated between can only being spent 0 to 90.

After the technical program, the design of the spacing block set nothing due to inordinate rotation after carrying or device are inverted Method returns to the problem of original state influence is normal to work.

As a further improvement, the heating module includes heat conduction shell, heating member, temperature sensor；The heating member It is arranged on temperature sensor inside heat conduction shell；Heating member and temperature sensor are electrically connected with control panel respectively；Heat conduction shell it is upper Limited block is provided with the mounting groove of fixation plummer of the surface provided with one side opening, mounting groove；Heat conduction shell is dismantled provided with convenient The Anchor plate kit two of plummer.

After the technical program, heating plate provides thermal source to plummer, and temperature sensor is setting heating and temperature control In fixed scope, limited block blocks plummer motionless, and Anchor plate kit two blocks the opening of heat conduction shell, solve plummer by Influence chip lid and the problem of covering effect of chip are subjected to displacement after pressure at opening.

Further to improve, Anchor plate kit two includes pressing plate two, steady pin and torsion spring；The opening pair of heat conduction shell and plummer It should locate to be provided with journal stirrup；Steady pin is fixed on journal stirrup；Pressing plate two is hinged and connected with heat conduction shell by steady pin；Torsion spring is fastened On steady pin, one end of torsion spring is connected in the torsional spring slot of pressing plate two, and the other end is connected on the heat conduction shell at journal stirrup；Pressing plate The opening of plummer is blocked stop plummer movement by two under torsion spring effect.

After the technical program, by press pressing plate two can be achieved between plummer and heat conduction shell it is quick fixed with Separation, the ecological chip for covering completion quickly can be taken out, rejoin new chip by aforesaid operations from plummer Afterwards, pressing plate two is stepped up into plummer again to prepare for next task.

Further to improve, retaining mechanism includes protecting cover, depression bar, guide rod and springs；The front of protecting cover is provided with fixation The fixing groove of chip lid；Provided with the through hole for facilitating guide rod activity at fixing groove；The back side of protecting cover, which is provided with, facilitates guide rod to move forward and backward Guiding groove and springs can in it deformation and the strip slot resetted；The two ends of strip slot are provided with to be carried out to springs Fixed stopper slot；Guiding groove is mutually perpendicular to strip slot；In the two ends clamping stopper slot of springs；The centre of springs Position is fixedly linked with guide rod；One end of depression bar is hinged and connected with matched moulds arm；The other end of depression bar is provided with breach；Guide rod is arranged on In guiding groove；One end of guide rod is stuck in depression bar breach, and the other end of guide rod is provided with fixture block；Fixture block is worn out of protecting cover through hole Go out, guide rod under the promotion of depression bar by deformation of the springs in strip slot or reset chip cover locking by fixture block or Open.

After the technical program, depression bar is pressed inward against, depression bar drives guide rod rearward displacement in through hole, chip lid is put Put in fixing groove, unclamp depression bar, by the reaction of springs, chip is fixed on by guide rod anterior displacement by fixture block In fixing groove, cover after completion, by moulded rod, clamping of the fixture block to chip lid is released after guide rod is moved afterwards again, and chip lid is stayed On plummer.Then matched moulds arm is inverted backward, reinstalls new chip lid and prepared for next task.

Brief description of the drawings

Fig. 1 is the schematic perspective view of digital pcr biochip sample adding device.

Fig. 2 be digital pcr biochip sample adding device hiding shell after schematic perspective view.

Fig. 3 is the schematic perspective view after the hiding shell and matched moulds arm of digital pcr biochip sample adding device.

Fig. 4 is the schematic diagram that digital pcr biochip sample adding device embodies fixed seat.

Fig. 5 is that digital pcr biochip sample adding device embodies the schematic diagram that lead screw motor is flexible coupling with connecting seat.

Fig. 6 is the sectional view of the sample-adding brush of digital pcr biochip sample adding device.

Fig. 7 is the stereogram one of the retaining mechanism of digital pcr biochip sample adding device.

Fig. 8 is the stereogram two of the retaining mechanism of digital pcr biochip sample adding device.

Wherein：1st, pedestal, 2, plummer, 3, sample-adding brush, 4, locating slot, 5, raised, 6, heating module, 7, sample charging mechanism, 8th, chip, 9, slow descending mechanism, 10, auxiliary descending mechanism, 11, control panel, 12, shell, 13, perforate, 14, power switch, 15, power supply inserts Mouthful, 16, by compressing switch, 17, diffuser, 18, LED, 19, matched moulds arm, 20, retaining mechanism, 21, chip lid, 22, screw rodb base, 23rd, fixed column, 24, optoelectronic switch, 25, optical coupled switch, 26, chip lid, 27, fixing groove, 31, buffer table, 61, heat conduction shell, 62nd, mounting groove, 63, limited block, 64, Anchor plate kit two, 65, journal stirrup, 71, adjutage, 72, connecting seat, 73, fixed seat, 74, rolling Dynamic bearing, 75, lead screw motor, 76, Anchor plate kit one, 77, boss, 78, inclined plane, 79, line slideway, 91, damper, 92, Damping spring, 93, fixing hole, 101, guide holder, 102, connecting rod, 103, plunger, 104, rail groove, 105, behaviour area, 106, lead To bar, 201, protecting cover, 202, depression bar, 203, guide rod, 204, springs, 211, guiding groove, 212, strip slot, 213, spacing Groove, 214, breach, 215, fixture block, 271, through hole, 641, pressing plate two, 711, spacing block set, 721, baffle plate, 731, connecting plate one, 732nd, contiguous block two, 733, chucking lug, 734, projection, 735, fixed ear, 761, pressing plate one, 762, block tongue.

Embodiment

The mode preferred to the utility model is further elaborated below in conjunction with the accompanying drawings：

As shown in Figures 1 to 8, a kind of digital pcr biochip sample adding device, including fixed plummer 2, sample-adding brush 3 and The pedestal 1 of chip lid, plummer is provided with the locating slot 4 of chip placement；It is provided with locating slot by being uniformly distributed that chip is held up Projection 5.Heating module 6：Thermal source is provided chip and by temperature stabilization in prescribed limit, heating module is fixedly installed on base One end of seat, plummer 2 is detachably secured to the top of heating module 6.The heating module 6 include heat conduction shell 61, heating member, Temperature sensor；In the technical program, heating member uses power resistor or PTC heating plates, temperature sensor using prioritizing selection Using NTC or PT100 or PT1000.The heating member and temperature sensor are arranged on inside heat conduction shell；Heating member and temperature are passed Sensor is electrically connected with control panel respectively；The mounting groove 62 of fixation plummer of the upper surface of heat conduction shell provided with one side opening, is installed Limited block 63 is provided with groove；Anchor plate kit two 64 of the heat conduction shell provided with convenient dismounting plummer.

Anchor plate kit 2 64 includes pressing plate 2 641, steady pin and torsion spring；The opening corresponding position of heat conduction shell and plummer is provided with Journal stirrup 65；Steady pin is fixed on journal stirrup；Pressing plate 2 641 is hinged and connected with heat conduction shell by steady pin；Torsion spring is fastened On steady pin, one end of torsion spring is connected in the torsional spring slot of pressing plate two, and the other end is connected on the heat conduction shell at journal stirrup；Pressing plate two The opening of plummer is blocked into stop plummer 2 under torsion spring effect to move.

Sample charging mechanism 7：It is fixed on pedestal 1, sample-adding brush 3 is removably fixedly linked with sample charging mechanism 7, sample-adding brush 3 exists Sample charging mechanism 7 is slow along after being moved straightly to close to above plummer 2 first under driving to be dropped to directly over chip 8.Sample-adding machine Structure 7 includes adjutage 71, connecting seat 72, fixed seat 73, rolling bearing 74 and spindle motor 75；The one end of adjutage 71 is with being connected Seat 72 is fixedly linked, and one end and the fixed seat 73 of fixed sample-adding brush 3 are fixedly linked；Fixed seat, which is provided with, is convenient for changing sample-adding brush 3 Anchor plate kit 1；Boss 77 of the bottom surface of adjutage provided with one section of inverted trapezoidal；Two sides of boss to the bottom of adjutage Inclined plane 78 is provided between face；Rolling bearing 74 is fixed on pedestal 1, and the bottom surface face of original state rolling bearing and boss connects Touch, as the mobile rolling bearing of adjutage excessively arrives the bottom plane-plane contact with adjutage by means of inclined plane；Lead screw motor is consolidated It is scheduled in pedestal, lead screw motor is electrically connected with control panel 11, and spindle motor is fixedly linked with connecting seat；The bottom of connecting seat is provided with With the rail groove for the mutual cooperation of line slideway 79 being fixed in pedestal；Connecting seat is under the drive of spindle motor relative to straight line The screw mandrel of guide rail and spindle motor advances or retreated.

Fixed seat 73 includes contiguous block 1 and contiguous block 2 732；Contiguous block one is fixedly linked with adjutage 71；Connection Mutually corresponding two side of block two is provided with chucking lug 733；Contiguous block two is by chucking lug with being fixed again after the clamping of contiguous block one It is connected；Contiguous block two is provided with projection 734；The projection corresponding position of sample-adding brush and contiguous block two is provided with locating slot；In sample-adding brush Between inclined buffer table 31 is provided with groove.

The Anchor plate kit 1 includes pressing plate 1, steady pin and torsion spring；Pressing plate 1 is provided with fixed sample-adding brush Block tongue 762；It is loaded brush 3 and is provided with neck corresponding with block tongue；Contiguous block 2 732 is provided with fixed ear 735；Steady pin is with consolidating Determine ear to be fixedly linked；Pressing plate 1 is hinged and connected with contiguous block 2 732 by steady pin；Torsion spring is fastened on steady pin, One end of torsion spring is connected in the torsional spring slot of pressing plate one, and the other end is connected at block tongue；Pressing plate one will sample-adding under torsion spring effect Brush is locked.

The screw mandrel of lead screw motor is provided with screw rodb base 22；Fixed column 23 and connecting seat that screw rodb base is made up of elastomeric material 72 are fixedly linked；Fixed column 23 is symmetricly set on the both sides of screw mandrel.

Slow descending mechanism 9：It is fixedly linked with sample charging mechanism 7, slows down the sinking speed of sample-adding brush 3；Slow descending mechanism 9 includes resistance Buddhist nun's device 91 and damping spring 92；Damper is fixedly linked with adjutage；Damper is provided with equally distributed fixing hole around central shaft 93；Damping spring is wrapped on damper；One end of damping spring is fixedly linked with connecting seat, and the other end is connected to damper In fixing hole.

Auxiliary descending mechanism 10：It is fixedly linked with sample charging mechanism, with the chip in locating slot 4 after 3 two landing of brush of auxiliary sample-adding It is in contact.The plunger 103 that auxiliary descending mechanism 10 includes guide holder 101, connecting rod 102 and elastomeric material are made；Guide holder is provided with rail Road groove 104 and behaviour area 105；The length of behaviour area is less than the length of boss 77；Rail groove adds the length of behaviour area to be more than boss Length；The height of rail groove is more than the height of connecting rod；One end of connecting rod 102 is hinged with adjutage to be fixedly linked, and the other end is set There is guide rod 106, original state guide rod is arranged on behaviour area；Plunger at one end is fixedly linked with connecting rod, the other end and adjutage It is fixedly linked；Original state plunger 103 is in compressive state.Adjutage 71 is provided with spacing block set 711, connecting rod in guide holder and Rotated between can only being spent under the cooperation of spacing block set 0 to 90.

Control panel 11：Control is provided to device, control panel 11 is electrically connected with heating module 6 and sample charging mechanism 7.Control panel 11 The optical coupled switch 25 of optoelectronic switch 24 and the arm rotation of monitoring matched moulds provided with control adjutage extended distance；The connecting seat 72 The baffle plate 721 started provided with triggering optoelectronic switch.

Shell 12：Sample charging mechanism 7, slow descending mechanism 9 and auxiliary descending mechanism 10 are covered in it after being fixedly linked with pedestal 1 and carried out Protection；Shell 12 is provided with the perforate 13 for facilitating sample charging mechanism to advance or retreat with the corresponding position of sample charging mechanism 7；On the side of shell 12 Provided with power switch 14 and outlet 15；The top of shell is provided with by 16 and the diffuser 17 of compressing switch；Diffuser 17 is arranged on The surface for the LED 18 being fixed on control panel 11 appears light；Power switch 14, outlet 15, by compressing switch 16 points Do not electrically connected with control panel 11；Matched moulds arm 19：It is hinged and connected by rotating shaft with pedestal 1；Matched moulds arm 19 is provided with fixed chip lid Retaining mechanism 20；Chip lid 21 is removably attached on retaining mechanism 20；Matched moulds arm rotates to plummer around the shaft will Chip lid is covered on chip.

Retaining mechanism 20 includes protecting cover 201, depression bar 202, guide rod 203 and springs 204；The front of protecting cover is provided with fixation The fixing groove 27 of chip lid 26；Provided with the through hole 271 for facilitating guide rod activity at fixing groove；The back side of protecting cover 201 is provided with and conveniently led The strip slot 212 that the movable guiding groove 211 of bar and springs 204 can be deformed and resetted in it；The two of strip slot End is provided with the stopper slot 213 that springs are fixed；Guiding groove is mutually perpendicular to strip slot；The two ends clamping of springs In stopper slot；The middle part of springs is fixedly linked with guide rod；In order to increase dynamics when guide rod resets, in strip slot A strip of sheet spring is also equipped with, the two ends of the strip of sheet spring are contradicted on the two side of strip slot respectively, the sheet bullet Spring is fixedly linked with guide rod.One end of depression bar is hinged and connected with matched moulds arm, and the other end of depression bar is provided with breach 214；In order to realize Depression bar can Rapid reset, also set up a strip of sheet spring on depression bar, one end and the depression bar of the springs are fixedly linked, another End offsets with matched moulds arm.Guide rod is arranged in guiding groove；One end of guide rod is stuck in depression bar breach, and the other end of guide rod is provided with card Block 215；Fixture block is passed out of protecting cover through hole, guide rod under the promotion of depression bar by deformation of the springs in strip slot or Reset and pass through fixture block by chip cover locking or opening.

The utility model is not limited to above-mentioned preferred forms, and anyone can draw under enlightenment of the present utility model Other various forms of products, however, make any change in its shape or structure, it is every that there is same as the present application or phase Approximate technical scheme, all falls within protection domain of the present utility model.

Claims (12)

1. a kind of digital pcr biochip sample adding device, includes the pedestal of fixed plummer (2), sample-adding brush (3) and chip lid (1), plummer is provided with the locating slot (4) of chip placement；Provided with the equally distributed projection for holding up chip in locating slot (5)；It is characterized in that：Also include heating module (6)：Thermal source is provided chip and by temperature stabilization in prescribed limit, heating Module is fixedly installed on one end of pedestal, and plummer (2) is detachably secured to the top of heating module (6)；Sample charging mechanism (7)： It is fixed on pedestal (1), sample-adding brush (3) is removably fixedly linked with sample charging mechanism (7), sample-adding brush (3) is in sample charging mechanism (7) It is slow along after being moved straightly to close to above plummer (2) first under drive to drop to directly over chip (8)；Slow descending mechanism (9)： It is fixedly linked with sample charging mechanism (7), slows down the sinking speed of sample-adding brush (3)；Auxiliary descending mechanism (10)：With sample charging mechanism stationary phase Even, it is in contact after (3) the secondary landing of auxiliary sample-adding brush with the chip in locating slot (4)；Control panel (11)：Control is provided to device System, control panel (11) is electrically connected with heating module (6) and sample charging mechanism (7)；Shell (12)：Will after being fixedly linked with pedestal (1) Sample charging mechanism (7), slow descending mechanism (9) and auxiliary descending mechanism (10) are covered on and protected in it；Shell (12) and sample charging mechanism (7) are right It should locate provided with the perforate (13) for facilitating sample charging mechanism to advance or retreat；The side of shell (12) is provided with power switch (14) and electricity Source socket (15)；The top of shell is provided with by compressing switch (16) and diffuser (17)；Diffuser (17), which is arranged on, is fixed on control The surface of LED (18) on plate (11) appears light；Power switch (14), outlet (15), by compressing switch (16) point Do not electrically connected with control panel (11)；Matched moulds arm (19)：It is hinged and connected by rotating shaft with pedestal (1)；Matched moulds arm (19) is provided with solid Determine the retaining mechanism (20) of chip lid；Chip lid (21) is removably attached on retaining mechanism (20)；Matched moulds arm revolves around the shaft Go at plummer and to cover chip lid on chip.

2. digital pcr biochip sample adding device according to claim 1, it is characterised in that：Sample charging mechanism (7) includes prolonging Semi-girder (71), connecting seat (72), fixed seat (73), rolling bearing (74) and spindle motor (75)；Adjutage (71) one end and company Joint chair (72) is fixedly linked, and one end and the fixed seat (73) of fixed sample-adding brush (3) are fixedly linked；Fixed seat is provided with and is convenient for changing The Anchor plate kit one (76) of sample-adding brush (3)；Boss (77) of the bottom surface of adjutage provided with one section of inverted trapezoidal；Two sides of boss To between the bottom surface of adjutage be provided with inclined plane (78)；Rolling bearing (74) is fixed on pedestal (1), original state rolling bearing With the bottom plane-plane contact of boss, as the mobile rolling bearing of adjutage excessively arrives the bottom surface face with adjutage by means of inclined plane Contact；Lead screw motor is fixed in pedestal, and lead screw motor is electrically connected with control panel (11), spindle motor and connecting seat stationary phase Even；The bottom of connecting seat is provided with the rail groove that the line slideway (79) with being fixed in pedestal cooperates；Connecting seat is in leading screw Screw mandrel under the drive of motor relative to line slideway and spindle motor advances or retreated.

3. digital pcr biochip sample adding device according to claim 2, it is characterised in that：Fixed seat (73) includes connecting Connect block one (731) and contiguous block two (732)；Contiguous block one is fixedly linked with adjutage (71)；Contiguous block two mutually corresponding two Side is provided with chucking lug (733)；Contiguous block two is by chucking lug with being fixedly linked again after the clamping of contiguous block one；On contiguous block two Provided with projection (734)；The projection corresponding position of sample-adding brush and contiguous block two is provided with locating slot；It is loaded to be provided with the intermediate groove of brush and inclines Oblique buffer table (31).

4. digital pcr biochip sample adding device according to claim 3, it is characterised in that：The Anchor plate kit one (76) pressing plate one (761), steady pin and torsion spring are included；Block tongue (762) of the pressing plate one (761) provided with fixed sample-adding brush；Sample-adding Brush (3) and be provided with neck corresponding with block tongue；Contiguous block two (732) is provided with fixed ear (735)；Steady pin is fixed with fixed ear It is connected；Pressing plate one (761) is hinged and connected with contiguous block two (732) by steady pin；Torsion spring is fastened on steady pin, torsion spring One end be connected in the torsional spring slot of pressing plate one, the other end is connected at block tongue；Pressing plate one is under torsion spring effect by sample-adding brush lock Fasten.

5. digital pcr biochip sample adding device according to claim 2, it is characterised in that：On the screw mandrel of lead screw motor Provided with screw rodb base (22)；The fixed column (23) that screw rodb base is made up of elastomeric material is fixedly linked with connecting seat (72)；Fixed column (23) both sides of screw mandrel are symmetricly set on.

6. digital pcr biochip sample adding device according to claim 2, it is characterised in that：Control panel (11) is provided with Control the optoelectronic switch (24) of adjutage extended distance and the optical coupled switch (25) of monitoring matched moulds arm rotation；The connecting seat (72) The baffle plate (721) started provided with triggering optoelectronic switch.

7. digital pcr biochip sample adding device according to claim 2, it is characterised in that：Slow descending mechanism (9) includes resistance Buddhist nun's device (91) and damping spring (92)；Damper is fixedly linked with adjutage；Damper is around central shaft provided with equally distributed solid Determine hole (93)；Damping spring is wrapped on damper；One end of damping spring is fixedly linked with connecting seat, and the other end is connected to resistance In the fixing hole of Buddhist nun's device.

8. digital pcr biochip sample adding device according to claim 2, it is characterised in that：Auxiliary descending mechanism (10) includes The plunger (103) that guide holder (101), connecting rod (102) and elastomeric material are made；Guide holder is provided with rail groove (104) and activity Area (105)；The length of behaviour area is less than the length of boss (77)；Rail groove adds the length of behaviour area to be more than the length of boss；Rail The height of road groove is more than the height of connecting rod；One end of connecting rod (102) is hinged with adjutage to be fixedly linked, and the other end is provided with guide rod (106), original state guide rod is arranged on behaviour area；Plunger at one end is fixedly linked with connecting rod, the other end and adjutage stationary phase Even；Original state plunger (103) is in compressive state.

9. digital pcr biochip sample adding device according to claim 8, it is characterised in that：Adjutage (71) is provided with Spacing block set (711), connecting rod is rotated between can only being spent under the cooperation of guide holder and spacing block set 0 to 90.

10. digital pcr biochip sample adding device according to claim 1, it is characterised in that：The heating module (6) Including heat conduction shell (61), heating member, temperature sensor；The heating member and temperature sensor are arranged on inside heat conduction shell；Heating Part and temperature sensor are electrically connected with control panel respectively；The installation of fixation plummer of the upper surface of heat conduction shell provided with one side opening Limited block (63) is provided with groove (62), mounting groove；Anchor plate kit two (64) of the heat conduction shell provided with convenient dismounting plummer.

11. digital pcr biochip sample adding device according to claim 10, it is characterised in that：Anchor plate kit two (64) Including pressing plate two (641), steady pin and torsion spring；The opening corresponding position of heat conduction shell and plummer is provided with journal stirrup (65)；Steady pin is consolidated It is scheduled on journal stirrup；Pressing plate two (641) is hinged and connected with heat conduction shell by steady pin；Torsion spring is fastened on steady pin, torsion spring One end be connected in the torsional spring slot of pressing plate two, the other end is connected on the heat conduction shell at journal stirrup；Pressing plate two is under torsion spring effect The opening of plummer is blocked into stop plummer (2) mobile.

12. digital pcr biochip sample adding device according to claim 4, it is characterised in that：Retaining mechanism (20) includes Protecting cover (201), depression bar (202), guide rod (203) and springs (204)；The front of protecting cover consolidating provided with fixed chip lid (26) Determine groove (27)；Provided with the through hole (271) for facilitating guide rod activity at fixing groove；The back side of protecting cover (201) is provided with before and after facilitating guide rod The strip slot (212) that mobile guiding groove (211) and springs (204) can be deformed and resetted in it；The two of strip slot End is provided with the stopper slot (213) that springs are fixed；Guiding groove is mutually perpendicular to strip slot；The two ends card of springs Connect in stopper slot；The middle part of springs is fixedly linked with guide rod；One end of depression bar is hinged and connected with matched moulds arm；Depression bar The other end is provided with breach (214)；Guide rod is arranged in guiding groove；One end of guide rod is stuck in depression bar breach, the other end of guide rod Provided with fixture block (215)；Fixture block is passed out of protecting cover through hole, and guide rod is under the promotion of depression bar by springs in strip slot Deformation or reset chip cover locking or opening by fixture block.