CN206418109U - The kit and Reagent Tube studied for immune group storehouse - Google Patents

The kit and Reagent Tube studied for immune group storehouse Download PDF

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Publication number
CN206418109U
CN206418109U CN201720079011.2U CN201720079011U CN206418109U CN 206418109 U CN206418109 U CN 206418109U CN 201720079011 U CN201720079011 U CN 201720079011U CN 206418109 U CN206418109 U CN 206418109U
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China
Prior art keywords
reagent
reagent tube
group
kit
pore
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CN201720079011.2U
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Chinese (zh)
Inventor
韩颖鑫
高晓峘
张印新
曾晓静
王佳伟
蒋馥蔓
李胜
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Guangzhou Jingke Medical Laboratory Co ltd
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Guangzhou Jingke Medical Laboratory Co ltd
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Abstract

The utility model is related to a kind of kit studied for immune group storehouse, and the kit includes:A drawer is set below box body, the box body;At least one Reagent Tube group is detachably placed with the box body, the surface of the Reagent Tube group sets Reagent Tube block graphics tag slot;The Reagent Tube group is provided with least one reagent pore, and the reagent pore is inserted and fixed for Reagent Tube.The utility model also provides a kind of Reagent Tube for mentioned reagent box.Design and detachable combination that the utility model passes through Reagent Tube group and reagent pore so that all types of reagents in kit are easily managed, and effectively can be shifted and be taken.And a drawer is set below kit, reagent can be incubated, it is not required that additionally look for ice chest or hold the box of ice cube, and without placing two kinds of boxes simultaneously, save the space of operation desktop according to the material such as placement ice cube the need for reagent.

Description

The kit and Reagent Tube studied for immune group storehouse
Technical field
The utility model is related to biochemistry detection technical field of tools, more particularly to a kind of to be used for what immune group storehouse was studied Kit, and the Reagent Tube for the kit.
Background technology
In biochemical field, biochemical material is carried out the experimental implementation such as to detect, is required to using a variety of different Reagent and consumptive material combine to realize the operation.It is in the art to there are many consumptive materials not of the same race to be used for depositing different types of reagent, Also have and the plurality of reagents that an experiment needs is put in same kit, be managed collectively and use.But, current reagent Putting for box is unordered mostly, in reagent simple arrangement to one or more box body that will be required, this arrangement mode Reagent bottle or a fairly large number of situation of Reagent Tube are not particularly suited for, easily occurs obscuring examination when reagent bottle or more Reagent Tube Agent so that detection cycle is elongated, and then experiment may be caused error or even failure, the accuracy of reduction detection data and inspection occur Survey efficiency.Higher experiment and the more experiment of use reagent number, available reagent box are required clearly for experimental precision Simple put can not meet this requirement.The related experiment of two generations sequencing largely belongs to molecular biology experiment, tests Required precision is very high, such as immune group storehouse is studied, and the experiment is by being enriched with and being sequenced many of φt cell receptor and B-cell receptor Sample is cloned, and the species and the frequency of every kind of clone cloned usually point out Immune system imbalance or the information tended to be balanced, Disease may be further pointed out, therefore it is required that experimentation wants accurate, it is impossible to occur causing because the mistake of reagent is used Detect the situation of the accuracy reduction of data.
Immune system is that body resists pathogen invasion and immunoregulatory important system, and the diversity of macromolecular is immunized Body is recognized and is removed countless foreign substances, remove the part metabolin that produces in vivo even apoptosis or aging Cell, and remain the balance of body immune system.Immune group storehouse refers to many as the important symbol of reaction immune system status The summation at the immunocyte of sample a certain moment in an individual, and these summations have been reacted individual inheritance factor, antigen and connect Touch the immunoregulation of history, surrounding environment and individual.Accurately obtain these information need to ensure experimentation be accurately, Therefore one it is easy to use and be avoided that the kit that reagent is obscured is necessary.Current experimentation is to use volume The ice chest of outer purchase is tested, or is put into ice cube in the box of definite shape, and Reagent Tube is put on reagent pipe support, then Related pipe support is placed on ice cube to ensure the low temperature of reagent together.It is so various using equipment, it is both inconvenient, also take a large amount of tables Space of planes.
Utility model content
In consideration of it, being necessary that offer is a kind of puts kit that is orderly, taking convenience, carry ice chest.
The utility model provides a kind of kit studied for immune group storehouse, and the kit includes:
An at least drawer is set below box body, the box body;
At least one Reagent Tube group is detachably placed with the box body, the surface of the Reagent Tube group sets Reagent Tube group Pattern identification area;
The Reagent Tube group is provided with least one reagent pore, and the reagent pore is inserted and fixed for Reagent Tube.
According to embodiment of the present utility model, at least one low temperature hole in the reagent pore, the low temperature hole is used Cryogenic material is prepared or is filled with cryogenic material, and low temperature is kept for the reagent in the Reagent Tube that makes to be inserted.
According to embodiment of the present utility model, the quantity in the low temperature hole is 1~3.
According to embodiment of the present utility model, the cryogenic material is ice crystal.
According to embodiment of the present utility model, the quantity of the reagent pore is 1~6.
According to embodiment of the present utility model, the Reagent Tube block graphics tag slot is arranged at the lower-left on Reagent Tube group surface Side, the Reagent Tube block graphics tag slot is colored geometrical pattern.Reagent Tube block graphics tag slot can make experimenter Intuitively each Reagent Tube group can be gone out by Fast Identification, upset even if Reagent Tube group puts order, will not also malfunction.
According to embodiment of the present utility model, the geometric figure includes triangle, rectangle, cylinder or hexagon.
According to embodiment of the present utility model, Reagent Tube group Digital ID area is set below the reagent pore, for pair Reagent pore is ranked up.The Reagent Tube group Digital ID area identify successively Roman number " I, II, III, IV, V, VI……”。
According to embodiment of the present utility model, the Reagent Tube group is provided with least one tag identifier area, for pasting Label paper.The label paper is used for the title or purposes for identifying corresponding each reagent.
According to embodiment of the present utility model, tag identifier area quantity is 1~3.
According to embodiment of the present utility model, the kit includes 3 group reagent pipe groups, and the Reagent Tube group is respectively RNA Reverse Transcription pipes group, digestion RNA Reagent Tubes group and PCR reagent pipe group.
According to embodiment of the present utility model, in addition to specification rest area, the specification rest area is arranged at described Reagent Tube group side.The specification rest area is used to place specification or operation manual.
According to embodiment of the present utility model, ice cube, ice bag are placed in the drawer.For keeping reagent in Reagent Tube group Pipe is in low-temperature condition.
This experiment is new also to include the Reagent Tube for mentioned reagent box, the Reagent Tube of the Reagent Tube and the kit Hole size is harmonious, and Reagent Tube Digital ID area and Reagent Tube pattern identification area, the Reagent Tube figure are provided with the Reagent Tube Shape tag slot is identical with the Reagent Tube block graphics tag slot of its corresponding Reagent Tube group placed, the Reagent Tube Digital ID area It is identical with its corresponding Reagent Tube group Digital ID area placed.The time of reagent identification can be so saved, and then shortens reagent The possibility of reagent is obscured in preparatory period and reduction.
According to embodiment of the present utility model, the Reagent Tube Digital ID area identify successively Roman number " I, II, III, IV、V、VI……”。
According to embodiment of the present utility model, the Reagent Tube block graphics tag slot is colored geometrical pattern, described Geometric figure includes rhombus, triangle, rectangle, cylinder or hexagon.
Relative to prior art, the utility model is combined by the design of Reagent Tube group and reagent pore with detachable, So that any Reagent Tube group only tests the reagent needed comprising a step in kit, and these reagents are walked according to the experiment set Suddenly the order used is put or put from big to small according to amount of reagent.It so may be such that all types of reagents are easy to pipe Reason, effectively can be shifted and be taken, save reagent time, improve the accuracy and efficiency of experiment.
It is of the present utility model that a drawer is set below kit, can be according to the placement material such as ice cube or ice bag the need for reagent Material, can keep low temperature, it is not required that additionally look for ice chest or hold the box of ice cube, and without placing two kinds of boxes simultaneously, Save the space of operation desktop.It may also be used for placing specification, operation manual etc..
Brief description of the drawings
Fig. 1 is the structural representation opened after drawer of the kit of the embodiment of the utility model one.
Fig. 2 is to close the structural representation after drawer in Fig. 1 embodiments.
Fig. 3 is the structural representation of RNA Reverse Transcription pipe groups in Fig. 1 embodiments.
Fig. 4 is the structural representation of digestion RNA Reagent Tube groups in Fig. 1 embodiments.
Fig. 5 is the structural representation of PCR reagent pipe group in Fig. 1 embodiments.
Fig. 6 is the structural representation of the Reagent Tube of the another embodiment of the utility model.
Fig. 7 is the structural representation of the kit of another embodiment of the utility model.
Main element symbol description
Kit 10、30
Box body 110
RNA Reverse Transcription pipe groups 120
Reagent pore 121
Reagent Tube group Digital ID area 122
Reagent Tube block graphics tag slot 123
Tag identifier area 124
Low temperature hole 125
Digest RNA Reagent Tube groups 130
PCR reagent pipe group 140
Drawer 150
Reagent Tube 20
Reagent Tube Digital ID area 201
Reagent Tube pattern identification area 202
Operational manual rest area 160
Following embodiment will further illustrate the utility model with reference to above-mentioned accompanying drawing.
Embodiment
In order that the purpose of this utility model, technical scheme and advantage are more clearly understood, below in conjunction with accompanying drawing and implementation Example, the utility model is further elaborated.It should be appreciated that specific embodiment described herein is only used for explaining this Utility model, is not used to limit the utility model.
It is a kind of to be used for the kit 10 that immune group storehouse is studied referring to Fig. 1 and Fig. 2 according to the utility model first embodiment, Including a box body 110, and multiple Reagent Tube groups being removably connected in box body 110.
Specifically, the Reagent Tube group includes a RNA Reverse Transcription pipes group 120, the He of a digestion RNA Reagent Tubes group 130 One PCR reagent pipe group 140.
The box body 110 is preferably its long 10.6cm, wide 3.9cm, high 5.5cm in rectangle, the present embodiment.
The box body 110 has a drawer 150, and the drawer 150 is arranged at the lower section of box body 110, the inner placement ice of drawer 150 Block, ice bag etc., for being incubated to reagent.
As shown in figure 3, the RNA Reverse Transcriptions pipe group 120 is located in box body 110, it is long against the top wall of box body 110 Flushed with height with box body, width is 1.85cm.
The RNA Reverse Transcriptions pipe group 120 includes 6 reagent pores 121, and pore is uniformly distributed in Reagent Tube group, A diameter of 1.2cm, the RNA Reverse Transcriptions pipe group 120 and RNA Reverse Transcription pipe sizes are harmonious, so that RNA reverse transcriptions are tried Agent pipe is inserted and fixed.The RNA Reverse Transcriptions are respectively reverse transcription primer working solution, deoxyribonucleoside triphosphate mixing Liquid, 1 chain reaction buffer solution, dithiothreitol (DTT) (DTT), RNase inhibitor and reverse transcriptase.
Last 2 pores are low temperature hole 125 in the RNA Reverse Transcriptions pipe group 120, are loaded using ice crystal, to insert Enter RNase inhibitor therein and reverse transcriptase reagent keeps low temperature.
The lower section of the reagent pore 121 sets Reagent Tube group Digital ID area 122, the Reagent Tube group Digital ID area 122 are followed successively by " I, II, III, IV, V, VI " from left to right.
The lower left on the surface of RNA Reverse Transcriptions pipe group 120 sets a Reagent Tube block graphics tag slot 123, described Reagent Tube block graphics tag slot 123 is triangle pattern, and preferably it is carried in colour code, the present embodiment, and it is yellow three Angular pattern.
The side of the RNA Reverse Transcriptions pipe group 120 is provided with 2 tag identifier areas 124, for adhesive label paper (not shown).
As shown in figure 4, the digestion RNA Reagent Tubes group 130 is located at the RNA Reverse Transcriptions pipe group 120 in box body 110 Lower right, it is long and it is high flushed with box body, width is 1.85cm.The structure of the digestion RNA Reagent Tubes group 130 substantially with institute State RNA Reverse Transcription pipe groups 120 identical.
The digestion RNA Reagent Tubes group 130 includes 1 reagent pore 121.The reagent pore is low temperature hole 125, is used Ice crystal is loaded, so that the digestion RNA reagents being inserted keep low temperature.
The lower section of the digestion RNA reagents pore 121 sets Reagent Tube group Digital ID area 122, the Reagent Tube group number Word tag slot 122 is " I ".
The lower left on the surface of digestion RNA Reagent Tubes group 130 sets Reagent Tube block graphics tag slot 123, the reagent Pipe block graphics tag slot 123 is cylinder pattern, and preferably it is carried in colour code, the present embodiment, and it is red cylinder pattern.
It will be appreciated by those skilled in the art that, because the only a kind of reagent of digestion RNA Reagent Tubes group 130, namely only one Reagent pore, it is possible to which the Reagent Tube group is directly connected with RNA Reverse Transcription pipes group 120.When experiment, to proceed to RNA anti- During transcription, RNA Reverse Transcription pipes group 120 and digestion RNA Reagent Tubes group 130 can be taken out in the lump.
As shown in figure 5, the PCR reagent pipe group 140 is located in box body 110 under the RNA Reverse Transcriptions pipe group 120 Side left side, long and height is flushed with box body, and width is 1.85cm.The structure of the PCR reagent pipe group 140 is substantially anti-with the RNA Transcript reagent pipe group 120 is identical.
The PCR reagent pipe group 140 includes 4 reagent pores 121, the PCR examinations that the PCR reagent pipe group 140 is loaded Agent is respectively PCR buffer solutions, PCR primer working solution 1, PCR primer working solution 2 and PCR enzyme intermixtures.
Last 1 pore in the PCR reagent pipe group 140 is low temperature hole 125, is loaded using ice crystal, to insert it In PCR enzymes intermixture keep low temperature.
The lower section of the PCR reagent pore 121 sets Reagent Tube group Digital ID area 122, the Reagent Tube group numeral mark Know area 122 and be followed successively by " I, II, III, IV " from left to right.
The lower left on the surface of PCR reagent pipe group 140 sets Reagent Tube block graphics tag slot 123, the Reagent Tube group Pattern identification area 123 is hexagonal shaped pattern, and preferably it is carried in colour code, the present embodiment, and it is green hexagonal shaped pattern. The side of the PCR reagent pipe group 140 is provided with 2 tag identifier areas 124.
Those skilled in the art are it should be understood that the quantity of the Reagent Tube group and reagent pore in each described Reagent Tube group can root The reagent type and quantity needed according to the experimental procedure is increased and decreased accordingly.
According to second embodiment of the present utility model, as shown in fig. 6, one is used for the Reagent Tube 20 of mentioned reagent box, it is described Reagent Tube 20 is harmonious with the size of reagent pore 121 in the Reagent Tube group, and Reagent Tube numeral is provided with the Reagent Tube 20 Tag slot 201 and Reagent Tube pattern identification area 202, the Reagent Tube pattern identification area 202 and its corresponding Reagent Tube placed The Reagent Tube block graphics tag slot 123 of group is identical, the Reagent Tube Digital ID area 201 and its corresponding reagent placed The Reagent Tube group Digital ID area 122 of pipe group is identical.
According to second embodiment of the present utility model, as shown in fig. 7, Reagent Tube group and first embodiment in the kit Identical, difference is also to include an operational manual rest area 160, and the operational manual rest area 160 is arranged at the examination Agent pipe group side.
It is understood that in the case where not needing multiple Reagent Tube groups, the kit can be only containing an examination Agent pipe group.
Above-described embodiment is the utility model preferably embodiment, but embodiment of the present utility model is not by above-mentioned The limitation of embodiment, embodiment of above is only for explaining claims.Right protection domain of the present utility model not office It is limited to specification.Any one skilled in the art can think easily in the technical scope that the utility model is disclosed The change or replacement arrived, are included within protection domain of the present utility model.

Claims (10)

1. a kind of kit studied for immune group storehouse, it is characterised in that the kit includes:
A drawer is set below box body, the box body;
Dismounting is placed with least one Reagent Tube group in the box body, and the surface of the Reagent Tube group sets Reagent Tube block graphics mark Know area;
The Reagent Tube group is provided with least one reagent pore, and the reagent pore is inserted and fixed for Reagent Tube.
2. kit according to claim 1, it is characterised in that at least one low temperature hole, institute in the reagent pore State low temperature hole to prepare using cryogenic material or be filled with cryogenic material, keep low for the reagent in the Reagent Tube that makes to be inserted Temperature.
3. kit according to claim 2, it is characterised in that the cryogenic material is ice crystal.
4. kit according to claim 1, it is characterised in that the Reagent Tube block graphics tag slot is arranged at Reagent Tube The lower left on group surface, the Reagent Tube block graphics tag slot is colored geometrical pattern.
5. kit according to claim 1, it is characterised in that the quantity of the reagent pore is 1~6.
6. kit according to claim 1, it is characterised in that Reagent Tube group numeral mark is set below the reagent pore Area is known, for being ranked up to reagent pore.
7. kit according to claim 1, it is characterised in that the kit includes 3 group reagent pipe groups, the reagent Pipe group is respectively RNA Reverse Transcription pipes group, digestion RNA Reagent Tubes group and PCR reagent pipe group.
8. kit according to claim 1, it is characterised in that also including specification rest area, the specification is placed Area is arranged at the Reagent Tube group side.
9. kit according to claim 1, it is characterised in that ice cube, ice bag are placed in the drawer.
10. a kind of Reagent Tube of kit for described in claim 1, the reagent pore of the Reagent Tube and the kit Size is harmonious, and Reagent Tube Digital ID area and Reagent Tube pattern identification area, the Reagent Tube figure are provided with the Reagent Tube Tag slot is identical with the Reagent Tube block graphics tag slot of its corresponding Reagent Tube group placed, the Reagent Tube Digital ID area with Its corresponding Reagent Tube group Digital ID area placed is identical.
CN201720079011.2U 2017-01-19 2017-01-19 The kit and Reagent Tube studied for immune group storehouse Active CN206418109U (en)

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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108774642A (en) * 2018-07-13 2018-11-09 南京市儿童医院 Purposes of the miR-214 as the biomarker of renal fibrosis in urine

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108774642A (en) * 2018-07-13 2018-11-09 南京市儿童医院 Purposes of the miR-214 as the biomarker of renal fibrosis in urine

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