CN206385161U - A kind of anaerobic culture device of liquid humid acid fertilizer bacterium - Google Patents
A kind of anaerobic culture device of liquid humid acid fertilizer bacterium Download PDFInfo
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- CN206385161U CN206385161U CN201621005249.2U CN201621005249U CN206385161U CN 206385161 U CN206385161 U CN 206385161U CN 201621005249 U CN201621005249 U CN 201621005249U CN 206385161 U CN206385161 U CN 206385161U
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Abstract
The utility model discloses a kind of anaerobic culture device of liquid humid acid fertilizer bacterium, reactor is set to the area of reactor I, the area of reactor II and the area of reactor III；By the combination in aeration tank and deoxygenation pond, by reduction-state AH2QDS is oxidized to AQDS, and electron transfer process is participated in again, realizes recycling for nutrient solution, reduces microbial inoculum cost of manufacture.The utility model is compared with traditional anaerobic culture device, it is to avoid the means such as topping up or manufacture vacuum environment, and operating method is simple；And after each cultivation cycle terminates, using bentonite adsorption microbial bacterial agent, it is to avoid microbial inoculum loss, for the effective using providing guarantee of follow-up microbial inoculum.
Description
Technical field
The utility model is the anaerobism training on microbial bacterial agent culture apparatus, more particularly to a kind of liquid humid acid fertilizer bacterium
Support device.
Background technology
Traditional microbiological anaerobic culture device mainly includes two kinds of forms：One is biochemical oxygen demand, i.e., by adding chemical drugs
Agent, using oxygen in consumption of chemical reaction device, manufactures anaerobic environment；Two be to use gas cylinder topping up into reactor
Or artificially manufacture vacuum environment.Liquid humid acid fertilizer bacterium is widely present in river drift and soil as a kind of anaerobic bacteria, and
Environment greatly differs from each other with actual bed mud-overlying water system in traditional anaerobic culture device；Secondly, reactor is combined with gas cylinder,
Not only increase floor space, improve microbial inoculum cost of manufacture, and reactor aerobic state is easily caused during per sub-sampling, it is impossible to protect
Demonstrate,prove strictly anaerobic environment.
Liquid humid acid fertilizer bacterium has the microorganism of humic formula respiration capability as a class, it is possible to use organic matter is used as carbon source
And electron donor, it is coupled energy and is grown.Reduction-state humus produced by humic formula respiration can be reduced further
Oxidation state species in environment, such as Fe (III), Mn (IV), Cr (VI), U (VI), nitroaromatic and polyhalo pollutant.
Therefore, humus respiration can influence the biogeochemical cycle of Carbon and nitrogen cycles and some trace metals in environment, and energy
Enough promote the detoxification of heavy metal and organic pollution, in-situ immobilization, dirt in the self-purification of water, contaminated soil and river drift
Had broad application prospects in terms of water process, and the current pilot scale culture for liquid humid acid fertilizer bacterium is still in blank rank
Section.
AQDS (electron transit mediator) is as a kind of humus pattern thing, with the quinonyl structure similar to natural humus,
But its molecular weight is far smaller than the humus in soil and deposit, it is easier to utilized by microorganism growth metabolism, 1mmol/
LAQDS can be coupled about 60 times of thalli growth.Therefore, the utility model designs a kind of Anaerobic culturel method, using AQDS nutrient solutions,
And recycle nutrient solution, it is ensured that the enrichment and growth of microbial inoculum is carried out under anaerobic environment.
The content of the invention
The purpose of this utility model, be in view of at present to the pilot scale culture of liquid humid acid fertilizer bacterium still in blank stage,
To overcome the shortcomings of the anaerobic culture device of traditional liquid humid acid fertilizer bacterium, there is provided a kind of liquid humid acid fertilizer bacterium efficiently concentrating culture
New equipment.
The utility model is achieved by following technical solution.
A kind of anaerobic culture device of liquid humid acid fertilizer bacterium, including reactor, automatic liquid-level control device, water distributor and remove
Oxygen pond；Characterized in that, the reactor is provided with the area of reactor I, the area of reactor II and the area of reactor III；
Unidirectional gas eduction unit 8 is provided with the top of the area of reactor I and activated sludge fills into mouth 9, the side in the area of reactor I
Face is provided with nutrient solution and fills into a mouthful A10, automatic liquid-level control device A11 and valve A12, and the valve A12 port of export is provided with admittedly
Liquid/gas separator A13, solid-liquid separator A13 are connected with the area of reactor II with aeration tank 19 respectively；
The side in the area of reactor II is provided with nutrient solution and fills into a mouthful B14, automatic liquid-level control device B15 and valve B16, valve
The door B16 port of export is provided with solid-liquid separator B17, and solid-liquid separator B17 is connected with the area of reactor III with aeration tank 19 respectively
It is logical；
The opposite side in the area of reactor II is provided with deoxygenation pond 3, and deoxygenation pond 3 is connected by centrifugal pump 4 with aeration tank 19；Remove
The top in oxygen pond 3 is provided with oxygen scavenger supply port 1 and automatic liquid-level control device 2, and deoxygenation pond 3 is by multidirectional valve 6 with setting
Water distributor A5 in the area of reactor II is connected with the water distributor B7 for being arranged on the area of reactor III；
The side in the area of reactor III is provided with automatic liquid-level control device C18；The bottom in the area of reactor III is provided with absorption
Area 21, adsorption zone 21 is connected by valve 20 with the area of reactor III；The side of adsorption zone 21 is provided with discharge gate 22, adsorption zone
21 bottom is provided with milipore filter 23；The top of milipore filter 23 is additionally provided with absorption carrier in adsorption zone 21, and the absorption carrier is
Bentonite.
The area of reactor I, the area of reactor II, the dischargeable capacity in the area of reactor III are respectively 0.2,0.2,0.4m3。
The area of reactor I, the gradient of the sections bottom dividing plate in the area of reactor II are 3%~5%.
The deoxygenation pond 3, the dischargeable capacity of aeration tank 19 are 0.2m3, the dischargeable capacity of adsorption zone 21 is 0.4m3。
The water distributor A5, water distributor B7 are the unilateral horizontal direction perforate in lower section, and opening diameter is 50mm, and quantity is
20。
The absorption carrier is the bentonite through grinding and crossing 80 mesh sieves and high-temperature sterilization, and bentonite addition is adsorption zone
The 1/4 of dischargeable capacity；And the medicament KH through grinding and crossing 80 mesh sieves and high-temperature sterilization is added wherein2PO4、CaCl2、MgSO4And
NaHCO3, the quality proportioning of medicament is 5：2：1：1, the volume of medicament addition is the 5~10% of the volume of bentonite addition,
It is laid in after medicament is well mixed with bentonite above milipore filter.
The utility model compensate for the blank of liquid humid acid fertilizer bacterium pilot scale culture device, and with traditional anaerobic culture device
Compare, it is to avoid the means such as topping up or manufacture vacuum environment, operating method is simple.In addition, by aeration tank and
The combination in deoxygenation pond, by reduction-state AH2QDS is oxidized to AQDS, and electron transfer process is participated in again, realizes the circulation of nutrient solution
Utilize, reduce microbial inoculum cost of manufacture.Meanwhile, the suction to microbial inoculum is realized using the stronger bentonite of adsorption capacity in adsorption zone
It is attached, it is to avoid microbial inoculum loss, for the effective using providing guarantee of follow-up microbial inoculum.
Brief description of the drawings
Fig. 1 is liquid humid acid fertilizer bacterium anaerobic culture device schematic diagram.
Reference is as follows：
1 --- oxygen scavenger supply port 2 --- automatic liquid-level control device
3 --- deoxygenation pond 4 --- centrifugal pumps
5 --- water distributor A 6 --- multidirectional valves
The unidirectional gas export mouths of 7 --- water distributor B 8 ---
Nutrient solution fills into a mouthful A to 9 --- activated sludge fills into mouth 10 ---
11 --- automatic liquid-level control device A 12 --- valve A
Nutrient solution fills into a mouthful B to 13 --- solid-liquid separator A 14 ---
15 --- automatic liquid-level control device B 16 --- valve B
17 --- solid-liquid separator B 18 --- automatic liquid-level control device C
19 --- aeration tank 20 --- valves
21 --- adsorption zone 22 --- discharge gates
23 --- milipore filter 24 --- bentonites
Embodiment
The utility model is prepared using conventional raw materials and common process method.
Bentonite is a kind of claystone, and the loose such as soil of quality can be dispersed into suspension or gel, therefore in aqueous medium
There is stronger adsorption capacity to liquid, organic substance, maximal absorptive capacity is up to 5 times of weight with itself.
Bentonite is general designation of the class using montmorillonite as the nonmetallic minerals of main mineral constituent, according to interlayer cation
Difference, is divided into sodium bentonite, calcium-base bentonite, hydrogen-based bentonite and organobentonite again.The adsorption capacity of sodium bentonite
Other species bentonites are substantially better than, therefore the preferred sodium bentonite of the utility model is as adsorbent, and after being ground
Used.
The operation principle of the utility model liquid humid acid fertilizer bacterium anaerobic culture device is as follows：
The complete cultivation cycle of specific embodiment is 96h, including three phases.First cultivation stage is 48h, in reaction
Carried out in the area of device I, dischargeable capacity is 0.2m3；Second cultivation stage is 24h, is carried out in the area of reactor II, and dischargeable capacity is
0.2m3；3rd cultivation stage is 24h, is carried out in the area of reactor III, dischargeable capacity is 0.2m3；After each cycle culture terminates,
It there are about 0.3m3Thalline and nutrient solution enter adsorption zone, adsorbed using bentonite.
Nutrient solution is AQDS nutrient solutions, and main component is beer waste water or molasses containing waste water, and supplement AQDS makees into waste water
For electron acceptor, magnitude of recruitment is controlled in 40~60g/m3, injection nutrient solution volume is 0.1m3, therefore the control of AQDS magnitude of recruitments is 6g.
(1) first cultivation stage, incubation time is 48h
Mouthful A10 is filled into the area of reactor I injection fresh medium by nutrient solution, when liquid level reaches automatic liquid level control
Stop during the setting height of device 11, i.e. I 1/2 volume of area of reactor processed.Take sewage plant anaerobic activated sludge as inoculation mother liquor, connect
The amount of kind is 0.08m3, activated sludge is by filling into culture mouth 9 enters the area of reactor I.In incubation, anaerobe is with lemon
Lemon acid sodium is carbon source and electron donor, and AQDS is that electron acceptor carries out quinone breathing, transmittance process of the electronics in cell membrane respiratory chain
In, coupling produces energy and supports thalli growth.With the proliferation and metabolism of liquid humid acid fertilizer bacterium, AQDS receives electronics and is reduced to
AH2QDS, nutrient solution gradually becomes orange red by yellow.The CH produced is excluded by unidirectional gas eduction unit 84、CO2And N2Deng
Gas.
Cultivate after 48h, mouthful B14 is filled into the area of reactor II injection fresh medium by nutrient solution, when liquid level reaches
Stop during to the setting height of automatic liquid-level control device 15, i.e. II 1/2 volume of area of reactor.Simultaneously open valve A12, thalline and
Nutrient solution enters the second cultivation stage culture of progress of the area of reactor II through solid-liquid separator A13, wherein thalline, and nutrient solution, which enters, to expose
Gas pond 19, when thalline and nutrient solution are gradually emptied, closes valve A12.It is 0.6m to control aeration quantity in aeration tank3/ h, aeration
Time 1h, in aeration process, nutrient solution gradually becomes yellow by orange red, wherein the AH being reduced2QDS is gradually oxidized to
AQDS, can participate in electron transmission, it is achieved thereby that nutrient solution is recycled as electron acceptor again.
In addition, after thalline and nutrient solution are gradually emptied in the area of reactor I, mouthful A10 is filled into reactor I by nutrient solution
Fresh medium is injected in area, when liquid level reaches automatic liquid-level control device A11 setting heights, the i.e. volume of Ith area of reactor 1/2
When stop.Activated sludge enters the area of reactor I by filling into mouth 9, and inoculum concentration is 0.08m3, a new round is restarted in the area of reactor I
The first stage culture of liquid humid acid fertilizer bacterium.
(2) second cultivation stages, incubation time is 24h
The timing since thalline enters the area of reactor II.
Nutrient solution in aeration tank 19 is pumped in deoxygenation pond 3 through centrifugal pump 4, and aeration tank (19) are effective with deoxygenation pond 3
Volume is 0.2m3；Using sulphite as oxygen scavenger in deoxygenation pond 3, added amount of chemical is 15g.Nutrient solution is in deoxygenation pond 3
2h is stopped, by automatic liquid-level control device 2 and multidirectional Valve controlling 6, through water distributor A5, water distributor B7, there is 1/2 volume respectively
Nutrient solution enters the area of reactor II and 1/2 volume nutrient solution enters the area of reactor III.Water distributor A5, water distributor B7 are that lower section is single
STH, and using Cyclic culture liquid from water distributor injection realize to lower section cultivate region hydraulic mixing, increase thalline with
The contact of nutrient solution, so as to improve phage surface mass-transfer efficiency.
After thalline culture 24h, open thalline and nutrient solution in valve B16, the area of reactor II and pass through solid-liquid separator B17,
Thalline enters the area of reactor III and carries out the 3rd cultivation stage culture；Nutrient solution enters aeration tank 19, and it is 0.6m to control aeration quantity3/
H, aeration time 1h, when thalline and nutrient solution are gradually emptied, close valve B16.
In addition, after thalline in the area of reactor II and nutrient solution are emptied, mouthful B14 is filled into the area of reactor II by nutrient solution
Fresh medium is injected, when liquid level reaches the setting height of automatic liquid-level control device 15, i.e. II 1/2 volume of area of reactor
Stop.
(3) the 3rd cultivation stages, incubation time is 24h
The timing since thalline enters the area of reactor III.
Nutrient solution in aeration tank is pumped to deoxygenation pond 3 through centrifugal pump 4, stops after 2h, by passing through automatic liquid level control
Device 2 and multidirectional Valve controlling 6, through water distributor B7, fully enter the area of reactor III.
After thalline culture 24h, open and be located at reactor bottom valve 20, thalline and nutrient solution enter adsorption zone 21, pass through
Automatic liquid-level control device 18 is controlled, when liquid flows to end, and closes valve 20.
Now, thalline is cultivated by 48h in the area of reactor I, opens valve A12, thalline and nutrient solution are through separation of solid and liquid
Device A13, wherein thalline enter the area of reactor II and carry out second stage culture, and nutrient solution is aerated into aeration tank 19, when anti-
When the thalline and nutrient solution in the areas of Ying Qi I are gradually emptied, valve A12 is closed；It is 0.3~0.6m to control aeration quantity in aeration tank 193/
H, 0.5~1h of aeration time, the continuous culture of liquid humid acid fertilizer bacterium is realized in repeat step (2), (3).
In addition, after thalline and nutrient solution are gradually emptied in the area of reactor I, mouthful A10 is filled into reactor I by nutrient solution
Fresh medium is injected in area, and liquid level is controlled by automatic liquid-level control device A11；Activated sludge fills into mouth by activated sludge
9 enter the area of reactor I, and inoculum concentration is the 30~40% of the area's dischargeable capacity of reactor I, and it is rotten that a new round is restarted in the area of reactor I
Grow the first stage culture of matter reducing bacteria.
The dischargeable capacity of adsorption zone 21 is 0.4m3, absorption carrier is provided with adsorption zone 21, absorption carrier is through grinding 80
The bentonite of mesh sieve and high-temperature sterilization, addition is 0.1m3；And add wherein through grinding 80 mesh sieves and high-temperature sterilization
Medicament KH2PO4、CaCl2、MgSO4、NaHCO3, mass ratio is 5：2：1：1, addition is the 5% of bentonite amount.
Thalline and nutrient solution open discharge gate 22 and collect thalline, waste liquid is through adsorption zone lower end after adsorption zone 21 is adsorbed
Milipore filter 23 is discharged.
Claims (5)
1. a kind of anaerobic culture device of liquid humid acid fertilizer bacterium, including reactor, automatic liquid-level control device, water distributor and deoxygenation
Pond；Characterized in that, the reactor is provided with the area of reactor I, the area of reactor II and the area of reactor III；
Unidirectional gas eduction unit (8) is provided with the top of the area of reactor I and activated sludge fills into mouth (9), the side in the area of reactor I
Face is provided with nutrient solution and fills into a mouthful A (10), automatic liquid-level control device A (11) and valve A (12), valve A (12) port of export
Solid-liquid separator A (13) is provided with, solid-liquid separator A (13) is connected with the area of reactor II with aeration tank (19) respectively；
The side in the area of reactor II is provided with nutrient solution and fills into a mouthful B (14), automatic liquid-level control device B (15) and valve B (16),
Valve B (16) port of export is provided with solid-liquid separator B (17), and solid-liquid separator B (17) is respectively with the area of reactor III with being aerated
Pond (19) is connected；
The opposite side in the area of reactor II is provided with deoxygenation pond (3), and deoxygenation pond (3) are connected by centrifugal pump (4) with aeration tank (19)
It is logical；Oxygen scavenger supply port (1) and automatic liquid-level control device (2) are provided with the top of deoxygenation pond (3), deoxygenation pond (3) are by more
It is connected to valve (6) with the water distributor A (5) for being arranged on the area of reactor II and the water distributor B (7) for being arranged on the area of reactor III；
The side in the area of reactor III is provided with automatic liquid-level control device C (18)；The bottom in the area of reactor III is provided with adsorption zone
(21), adsorption zone (21) is connected by valve (20) with the area of reactor III；The side of adsorption zone (21) is provided with discharge gate
(22), the bottom of adsorption zone (21) is provided with milipore filter (23)；Suction is additionally provided with above adsorption zone (21) interior milipore filter (23)
Appendix body, the absorption carrier is bentonite.
2. a kind of anaerobic culture device of liquid humid acid fertilizer bacterium according to claim 1, it is characterised in that the reactor
Ith area, the area of reactor II, the dischargeable capacity in the area of reactor III are respectively 0.2,0.2,0.4m3。
3. a kind of anaerobic culture device of liquid humid acid fertilizer bacterium according to claim 1, it is characterised in that the reactor
Ith area, the gradient of the sections bottom dividing plate in the area of reactor II are 3%~5%.
4. a kind of anaerobic culture device of liquid humid acid fertilizer bacterium according to claim 1, it is characterised in that the deoxygenation pond
(3), aeration tank (19) dischargeable capacity is 0.2m3, adsorption zone (21) dischargeable capacity is 0.4m3。
5. a kind of anaerobic culture device of liquid humid acid fertilizer bacterium according to claim 1, it is characterised in that the water distributor
A (5), water distributor B (7) are the unilateral horizontal direction perforate in lower section, and opening diameter is 50mm, and quantity is 20.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201621005249.2U CN206385161U (en) | 2016-08-30 | 2016-08-30 | A kind of anaerobic culture device of liquid humid acid fertilizer bacterium |
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| CN201621005249.2U CN206385161U (en) | 2016-08-30 | 2016-08-30 | A kind of anaerobic culture device of liquid humid acid fertilizer bacterium |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106167763A (en) * | 2016-08-30 | 2016-11-30 | 天津大学 | The anaerobic culture device of liquid humid acid fertilizer bacterium |
| WO2022160571A1 (en) * | 2021-01-29 | 2022-08-04 | 上海睿钰生物科技有限公司 | In vitro life culture system and method |
-
2016
- 2016-08-30 CN CN201621005249.2U patent/CN206385161U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106167763A (en) * | 2016-08-30 | 2016-11-30 | 天津大学 | The anaerobic culture device of liquid humid acid fertilizer bacterium |
| WO2022160571A1 (en) * | 2021-01-29 | 2022-08-04 | 上海睿钰生物科技有限公司 | In vitro life culture system and method |
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| AV01 | Patent right actively abandoned | ||
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Granted publication date: 20170808 Effective date of abandoning: 20180817 |