CN206352133U - Nucleic acid-extracting apparatus - Google Patents
Nucleic acid-extracting apparatus Download PDFInfo
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- CN206352133U CN206352133U CN201621124305.4U CN201621124305U CN206352133U CN 206352133 U CN206352133 U CN 206352133U CN 201621124305 U CN201621124305 U CN 201621124305U CN 206352133 U CN206352133 U CN 206352133U
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- solution conduit
- nucleic acid
- solution
- branch pipe
- extracting apparatus
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Abstract
The utility model discloses a kind of nucleic acid-extracting apparatus, it belongs to nucleic acid extraction technical field, including：The two-way pump being connected with the first branch pipe and the second branch pipe；Magnet accessible and away from the second branch pipe；The solution conduit being connected with the first branch pipe and the second branch pipe；The normal closed gate of the control solution conduit break-make is provided with each solution conduit.This nucleic acid-extracting apparatus with solution conduit place nucleic acid extraction during needed for solution, and by two-way pump control solution in solution conduit and branch Bottomhole pressure, so as to realize the extraction of nucleic acid.The nucleic acid-extracting apparatus is simple in construction, it is easy to operate, solution conduit clear layer in parallel, it is the purifying and extraction of achievable nucleic acid with a simple device, whole extraction process does not contact personnel, while producing the phenomenon of aerosol when avoiding mixing, the device also allows for the automation mechanized operation of sample.
Description
Technical field
The utility model is related to nucleic acid extraction technical field, more particularly to a kind of nucleic acid-extracting apparatus.
Background technology
Nucleic acid is the same with protein, is also large biological molecule.The relative molecular mass of nucleic acid is very big, and usually hundreds of thousands is extremely
It is millions of.Many nucleotides are obtained after nucleic acid hydrolysis, it is demonstrated experimentally that nucleotides is the base unit for constituting nucleic acid, that is, core are constituted
The monomer of acid molecule.One nucleic acid molecule is made up of the nitrogenous base of a molecule, a molecule pentose and a molecule phosphoric acid
's.Nucleotides can be divided into by deoxyribonucleotide and ribonucleotide according to the difference of pentose.
Nanometer magnetic bead is the super-paramagnetism nano microballoon that a kind of surface carries carboxyl, is stably dispersed in water and PH>6.5
Biological buffer solutions in (such as phosphate, Tris, carbonate, borate buffer solution).Its surface is rich in carboxyl, and the carboxyl can
With biological aglucon covalent bond, while because it has superparamagnetism, quick separating can be realized under externally-applied magnetic field, therefore can be extensively
Applied to fields such as biomarker, immunodiagnosis, protein separation, cell separation, immobilised enzymes, biology sensors, especially
It is applied to high throughput automated platform.
Beads enrichment extractive technique (lower to be referred to as " paramagnetic particle method ") is with surface of the nanometer technology to superparamagnetic nano particle
Special chemical group is modified, there is extremely strong specific affinity to nucleic acid molecules under certain condition, and when conditions change,
Magnetic bead can be with reversible release nucleic acid, so that fast separating and purifying nucleic acid, and can remove isolating protein to greatest extent and other miscellaneous
Matter, so as to ensure to extract the purity of nucleic acid.
In the prior art, the scientific research personnel in laboratory during " paramagnetic particle method " nucleic acid extraction is utilized usually using really
The deep-well plates that bottom common in room is U-shaped structure are tested, the monoblock magnet bought by the market is carried out from bottom or side
Separation.
Conventional instrument for extracting nucleic acid, uses deep-well plates as container, it is therein as a kind of open type device more at present
Organic solution is readily volatilized, working environment and human body can be impacted and injured.In addition, when sample is mixed with solution, by
In strenuous exercise, aerosol is easily produced, sample and environment are polluted.The utility model is exactly that manufacture is a kind of totally enclosed
Device, is prevented effectively from above mentioned problem.
Utility model content
The purpose of this utility model is to provide a kind of closed, integral type, nucleic acid-extracting apparatus simple in construction.
For up to this purpose, the utility model uses following technical scheme：
A kind of nucleic acid-extracting apparatus includes：
First branch pipe and the second branch pipe；
Two-way pump, it is connected with first branch pipe and the second branch pipe；
Magnet, it is set in the way of accessible and remote second branch pipe；
It is connected with first branch pipe and the second branch pipe and multiple solution conduits for being used to place solution parallel with one another；
The normal closed gate of the control solution conduit break-make is provided with each solution conduit.
Preferably, the solution conduit is five, the first solution conduit of liquid is respectively combined for placing cracking, for putting
Put deproteinized cleaning solution the second solution conduit, for place go lipid cleaning solution the 3rd solution conduit, for place desalt, alcohols
4th solution conduit of cleaning solution, the 5th solution conduit for placing eluent.
Preferably, one end of first branch pipe and the second branch pipe is provided with first solution conduit, the other end is set
There is the two-way pump；Second solution conduit, the described 3rd are followed successively by from first solution conduit to the direction of the two-way pump
Solution conduit, the 4th solution conduit, the 5th solution conduit.
Preferably, setting having heaters in first solution conduit.
Preferably, setting having heaters in the 5th solution conduit.
Preferably, the top of first solution conduit is provided with first opening to be opened/closed.
Preferably, offering the second opening to be opened/closed in the 5th solution conduit.
Preferably, the magnet is permanent magnet or electromagnet.
Preferably, the normal closed gate is hand-operated valve or magnetic valve.
Preferably, when the nucleic acid-extracting apparatus by use, the solution conduit perpendicular to operation post plane or with work
The plane of industry platform is oblique.
Nucleic acid-extracting apparatus provided by the utility model, its with solution conduit place nucleic acid extraction during needed for it is molten
Liquid, and solution is controlled in solution conduit and branch Bottomhole pressure by two-way pump, so as to realize the extraction of nucleic acid.The nucleic acid-extracting apparatus
Solution conduit clear layer simple in construction, it is easy to operate, in parallel, and then make it that the extraction process of nucleic acid is safe, prevent gas
Colloidal sol and environmental pollution, device are easy, automation mechanized operation preferably.
Brief description of the drawings
Fig. 1 is the schematic diagram for the nucleic acid-extracting apparatus that the utility model embodiment is provided；
In figure：The branch pipes of 1- first, the branch pipes of 2- second, 3- two-way pumps, 4- first are open, the solution conduits of 5a- first, 5b- second
Solution conduit, the solution conduits of 5c- the 3rd, the solution conduits of 5d- the 4th, the solution conduits of 5e- the 5th, 6- normal closed gates, 7- magnet, 8- second are opened
Mouthful.
Embodiment
Further illustrate the technical solution of the utility model below in conjunction with the accompanying drawings and by embodiment.
As shown in figure 1, a kind of nucleic acid-extracting apparatus include the first branch pipe 1, it is the second branch pipe 2, two-way pump 3, solution conduit, normally closed
Valve 6 and magnet 7.Wherein, the first branch pipe 1 and the second branch pipe 2 be arranged in parallel, the branch pipe 1 of two-way pump 3 and first and the second branch pipe 2
It is connected.Magnet 7 is set in the way of accessible and remote second branch pipe 2, multiple solution conduits and first for being used to place solution
Branch pipe 1 is connected with the second branch pipe 2 and each solution conduit is parallel with one another, and the control solution conduit is provided with each solution conduit
The normal closed gate 6 of break-make, the normal closed gate 6 is hand-operated valve or magnetic valve.Hand-operated valve is easy to operating personnel's operation, and magnetic valve is easy
Automatically controlled in realization.
A kind of embodiment, as shown in figure 1, solution conduit is five, respectively combines the first solution of liquid for placing cracking
Pipe 5a, the second solution conduit 5b for placing deproteinized cleaning solution, for place split lipid cleaning solution the 3rd solution conduit 5c,
For place desalt, the 4th solution conduit 5d of alcohols cleaning solution, the 5th solution conduit 5e for placing eluent.Each solution conduit
And first normal closed gate 6 for being provided with the corresponding solution conduit of a control and the break-make of the first branch pipe 1 between branch pipe 1.Certainly, it is normally closed
Valve 6 may also be arranged between each solution conduit and the second branch pipe 2, also or, normal closed gate 6 may also be arranged on the second branch pipe 2
Between two-way pump 3.
One end of first branch pipe 1 and the second branch pipe 2 is provided with the first solution conduit 5a, and the other end is provided with two-way pump 3；From
The direction of one solution conduit 5a to two-way pump 3 is followed successively by the second solution conduit 5b, the 3rd solution conduit 5c, the 4th solution conduit 5d, the 5th molten
Liquid pipe 5e.
When the nucleic acid-extracting apparatus is by use, plane of the solution conduit perpendicular to the plane of operation post or with operation post
Obliquely, while ensuring that solution does not produce dead volume in the running.It is to utilize gravity during mixing, is disturbed with reference to bubble
Dynamic progress liquid mixing.
The process for extracting nucleic acid is as follows：
Injection deproteinized cleaning solution, the 3rd solution conduit in the first solution conduit 5a implosion solution combination liquid, the second solution conduit 5b
Lipid cleaning solution is removed in injection in 5c, and the interior injections of the 4th solution conduit 5d are desalted, alcohols cleaning solution, injection elution in the 5th solution conduit 5e
Original state just contains magnetic bead in liquid in liquid, and above-mentioned first solution conduit 5a.
Determinand is injected into the first solution conduit 5a, and the first solution conduit 5a is heated at a temperature of 80 DEG C or so；
First solution conduit 5a top is provided with first opening 4 to be opened/closed, and when that need to inject determinand, first opening 4 is decontroled
Open, be usually then in closed state.
Open and be arranged on normal closed gate 6 between the first branch pipe 1 and the first solution conduit 5a, magnet 7 away from the second branch pipe 2,
Start two-way pump 3 and reversion, using gravity, allow the air of the second branch pipe 2 to enter and bubble is produced in the first solution conduit 5a, disturb molten
Liquid, allows two solution to be sufficiently mixed in 5a；
Magnet 7 is rotated forward close to the second branch pipe 2, two-way pump 3, and the liquid in the first solution conduit 5a is flowed in two-way pump 3, liquid
Magnetic bead adsorbed by magnet 7, this reaction time is about 2 minutes or so；
Two-way pump 3 is inverted, and utilizes gravity, it is to avoid liquid backflow reenters branch pipe 1；
The normal closed gate 6 being arranged between the first branch pipe 1 and the first solution conduit 5a is closed, the first branch pipe 1 and second is opened
Normal closed gate 6 between solution conduit 5b；
Two-way pump 3 continues to rotate forward, and the liquid in the second solution conduit 5b flows to two-way pump 3, while magnet 7 is away from the second branch pipe
Solution in 2, the second solution conduit 5b is mixed with magnetic bead, and this reaction time is about 1-2 minutes；
Two-way pump 3 is inverted, and liquid is flowed back in the second solution conduit 5b, is sufficiently mixed cleaning solution and is subject to deproteinized；
Magnet 7 rotates forward liquid and flows to two-way pump 3 close to the second branch pipe 2, two-way pump 3, and this reaction time is about 2 minutes left sides
It is right；；
Two-way pump 3 is inverted, and fluid is flowed back in the second solution conduit 5b；
The normal closed gate 6 closed between the first branch pipe 1 and the second solution conduit 5b, opens the 3rd solution conduit 5c and the first branch pipe
Normal closed gate 6 between 1；
Two-way pump 3 continues to rotate forward, and the liquid in the 3rd solution conduit 5c flows to two-way pump 3, while magnet 7 is away from the second branch pipe
2, this reaction time is 1-2 minutes；
Two-way pump 3 is inverted, and liquid is flowed back in the 3rd solution conduit 5c, is sufficiently mixed cleaning solution and is gone lipid；
Magnet 7 rotates forward liquid and flows to two-way pump 3 close to the second branch pipe 2, two-way pump 3, and this reaction time is about 2 minutes left sides
It is right；
Two-way pump 3 is inverted, and fluid is flowed back in the 3rd solution conduit 5c；
The normal closed gate 6 closed between the 3rd solution conduit 5c and the first branch pipe 1, opens the 4th solution conduit 5d and the first branch pipe
Normal closed gate 6 between 1；
Two-way pump 3 continues to rotate forward, and the liquid in the 4th solution conduit 5d flows to two-way pump 3, while magnet 7 is kept close to second
Branch pipe 2, this reaction time is 1-2 minutes；
Two-way pump 3 is inverted, and liquid is flowed back in the 4th solution conduit 5d, be sufficiently mixed cleaning solution desalted, alcohols, and clean
Pipeline；
The normal closed gate 6 closed between the 4th solution conduit 5d and the first branch pipe 1, opens the first branch pipe 1 and the 5th solution conduit
Normal closed gate 6 between 5e, and the 5th solution conduit 5e is heated at a temperature of 50-60 DEG C or so；
Magnet 7 is rotated forward away from the second branch pipe 2, two-way pump 3, and the eluent in the 5th solution conduit 5e flows to two-way pump 3, reaction
Time is 1-2 minutes；
Two-way pump 3 is inverted, and liquid is flowed back in the 5th solution conduit 5e, is sufficiently mixed eluent, and the reaction time is 5-6 minutes,
Magnetic bead and nucleic acid are separated；
The second opening 8 to be opened/closed is offered on 5th solution conduit 5e, so that solution is shifted.
For the ease of being heated to the first solution conduit 5a and the 5th solution conduit 5e, then, the first solution conduit 5a and the 5th
Heater is provided with solution conduit 5e.
In summary, the solution needed for this nucleic acid-extracting apparatus is placed during nucleic acid extraction with solution conduit, and passes through
Two-way pump controls solution in solution conduit and branch Bottomhole pressure, so as to realize the extraction of nucleic acid.The nucleic acid-extracting apparatus is simple in construction,
Easily operated, in parallel solution conduit clear layer, is the purifying and extraction of achievable nucleic acid, whole extraction with a simple device
Process does not contact personnel, safe, reduces the pollution between sample and environment, is easy to automation mechanized operation.
Obviously, above-described embodiment of the present utility model is used for the purpose of clearly illustrating the utility model example, and
It is not the restriction to embodiment of the present utility model.For those of ordinary skill in the field, in described above
On the basis of can also make other changes in different forms.There is no need and unable to give all embodiments
It is exhaustive.All any modifications, equivalent substitutions and improvements made within spirit of the present utility model and principle etc., should be included in
Within the utility model scope of the claims.
Claims (10)
1. a kind of nucleic acid-extracting apparatus, it is characterised in that including：
First branch pipe and the second branch pipe；
Two-way pump, it is connected with first branch pipe and the second branch pipe；
Magnet, it is set in the way of accessible and remote second branch pipe；
It is connected with first branch pipe and the second branch pipe and multiple solution conduits for being used to place solution parallel with one another；
The normal closed gate of the control solution conduit break-make is provided with each solution conduit.
2. nucleic acid-extracting apparatus according to claim 1, it is characterised in that：The solution conduit is five, is respectively used for
Place and crack the first solution conduit for combining liquid, the second solution conduit for placing deproteinized cleaning solution, go lipid to wash for placing
Wash liquid the 3rd solution conduit, for place desalt, the 4th solution conduit of alcohols cleaning solution, the 5th solution for placing eluent
Pipe.
3. nucleic acid-extracting apparatus according to claim 2, it is characterised in that：One end of first branch pipe and the second branch pipe
First solution conduit is provided with, the other end is provided with the two-way pump；From first solution conduit to the side of the two-way pump
To being followed successively by second solution conduit, the 3rd solution conduit, the 4th solution conduit, the 5th solution conduit.
4. nucleic acid-extracting apparatus according to claim 3, it is characterised in that：Heating is provided with first solution conduit
Device.
5. nucleic acid-extracting apparatus according to claim 3, it is characterised in that：Heating is provided with 5th solution conduit
Device.
6. nucleic acid-extracting apparatus according to claim 2, it is characterised in that：The top of first solution conduit is provided with one
First opening to be opened/closed.
7. nucleic acid-extracting apparatus according to claim 2, it is characterised in that：Offer and can be opened and closed in 5th solution conduit
Second opening.
8. nucleic acid-extracting apparatus according to claim 1, it is characterised in that：The magnet is permanent magnet or electromagnet.
9. nucleic acid-extracting apparatus according to claim 1, it is characterised in that：The normal closed gate is hand-operated valve or electromagnetism
Valve.
10. nucleic acid-extracting apparatus according to claim 1, it is characterised in that：When the nucleic acid-extracting apparatus is by use, institute
State solution conduit perpendicular to the plane of operation post or oblique with the plane of operation post.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621124305.4U CN206352133U (en) | 2016-10-14 | 2016-10-14 | Nucleic acid-extracting apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621124305.4U CN206352133U (en) | 2016-10-14 | 2016-10-14 | Nucleic acid-extracting apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206352133U true CN206352133U (en) | 2017-07-25 |
Family
ID=59346316
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621124305.4U Active CN206352133U (en) | 2016-10-14 | 2016-10-14 | Nucleic acid-extracting apparatus |
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| CN (1) | CN206352133U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113308364A (en) * | 2021-06-09 | 2021-08-27 | 北京京东方技术开发有限公司 | Nucleic acid extraction substrate, nucleic acid extraction apparatus, and nucleic acid extraction method |
| CN113528333A (en) * | 2021-07-20 | 2021-10-22 | 北京擎科生物科技有限公司 | Nucleic acid synthesis reaction apparatus and nucleic acid synthesis method |
-
2016
- 2016-10-14 CN CN201621124305.4U patent/CN206352133U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113308364A (en) * | 2021-06-09 | 2021-08-27 | 北京京东方技术开发有限公司 | Nucleic acid extraction substrate, nucleic acid extraction apparatus, and nucleic acid extraction method |
| CN113528333A (en) * | 2021-07-20 | 2021-10-22 | 北京擎科生物科技有限公司 | Nucleic acid synthesis reaction apparatus and nucleic acid synthesis method |
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