Cell culture incubator
Technical field
The utility model is related to cell culture, more particularly to cell culture incubator.
Background technology
The growing environment good to ensure cell, the culture region of cell or operating area need to keep certain gas
Concentration (carbon dioxide or carbon dioxide and oxygen).Current gas control mode mainly using the gas for rising concentration and drops dense
The gas of degree controls the concentration of internal gas by pid algorithm.
But such technology is primarily present following deficiency:Due to during liter concentration or drop concentration, in order in keeping
The stabilization of portion's pressure, the injection meeting of gas causes the waste of injection gas along with the discharge of gas;Reduce the effect of control
Rate.
Utility model content
To solve the deficiency in above-mentioned prior art, the utility model provides that a kind of gas consumption is small, gas
The high cell culture incubator of concentration control efficiency.
The purpose of this utility model is achieved through the following technical solutions:
A kind of cell culture incubator, the cell culture incubator includes housing;The cell culture incubator further comprises:
Supply unit, the supply unit is used to the gas in the housing being transported to regulation device;
Regulation device, the regulation device is respectively at the storage of object gas, release according to the difference of running parameter
State, the output end of the regulation device connects the enclosure interior;
The content of object gas in the housing measured is sent to processor by sensor, the sensor;
Processor, the processor controls the working condition of the regulation device according to the content received.
Prior art is compared, the utility model have the advantage that for:
1. reduce the loss of gas;
2. accelerate the efficiency of gas control;
3. the hot gas set protection interlayer of internal layer periphery, the temperature in the gas inside internal layer, interlayer is heated by air circulation
Degree is higher than inside internal layer, effectively prevents that condensed water from occurring in internal layer inwall, reduces contaminated risk;
4. cell culture operations are integrated, the culture amount of cell is effectively increased.
Brief description of the drawings
Referring to the drawings, disclosure of the present utility model will be easier to understand.Skilled addressee readily understands that
It is:These accompanying drawings are used only for illustrating the technical solution of the utility model, and are not intended to protection of the present utility model
Scope is construed as limiting.In figure:
Fig. 1 is the structure diagram of the cell culture incubator according to the utility model embodiment 1;
Fig. 2 is the structure diagram of the cell culture incubator according to the utility model embodiment 3.
Embodiment
Fig. 1-2 and following description describe optional embodiment of the present utility model how to instruct those skilled in the art
Implement and reproduce the utility model.In order to instruct technical solutions of the utility model, simplify or eliminate some conventional aspects.This
Art personnel should be appreciated that modification or replacement from these embodiments will be in the range of the utility model.This area
Technical staff should be appreciated that following characteristics can combine to form multiple modifications of the present utility model in a variety of ways.Thus, originally
Utility model is not limited to following optional embodiments, and is only limited by claim and their equivalent.
Embodiment 1:
Fig. 1 schematically illustrates the structure diagram of the cell culture incubator of the utility model embodiment, as shown in figure 1, institute
Stating cell culture incubator includes:
Housing, the housing has gas access, and the double-decker constituted for internal layer, outer layer;The upper wall of the internal layer
There is through hole with lower wall;
Supply unit, such as blower fan, the supply unit are used to the gas in the housing being transported to regulation device, specifically
It is arranged on the downside of the through hole of the upper wall of the internal layer;
Regulation device, the regulation device is respectively at the storage of object gas, release according to the difference of running parameter
State, the output end of the regulation device connects the enclosure interior;The regulation device is 2, is oxygen molecule sieve, dioxy
Change carbon molecular sieve, adsorption and desorption are carried out to gas molecule using pressure swing adsorption (PSA), so as to store, discharge target
Gas;Regulation and control are arranged in the interlayer on the top of the double-decker;
Filter, the filter is arranged on the bottom of the supply unit, the projection of the filter in the horizontal plane
The figure surrounded with the inwall of the internal layer on the horizontal plane is overlapped;
Oxygen in the housing measured, the content of carbon dioxide are sent to processor by sensor, the sensor;
Processor, the processor controls the working condition of the regulation device according to the content received:If less than mesh
Scale value, makes regulation device be in release conditions by control;If higher than desired value, making regulation device be in storage shape by control
State.
The control method of the cell culture incubator of the utility model embodiment, namely above-mentioned cell culture incubator method of work,
The control method of the cell culture incubator comprises the following steps:
(A1) content of the object gas such as oxygen, carbon dioxide in gas sensor detection cell culture incubator internal layer, and
It is sent to processor;
(A2) processor goes to control the working condition of regulation device according to the content received:
If desired for the content of reduction object gas, into step (A3);
If desired for the content of rise object gas, into step (A4);
As that need not adjust, into step (A5);
(A3) parameter of the regulation device is controlled, regulation device is in object gas storing state;In supply unit
In the presence of, gas enters in interlayer from the lower wall of internal layer, is flowed up to along the interlayer between the wall of side in the interlayer between upper wall,
Object gas in gas is adjusted device storage, finally enters from the upper wall of internal layer inside internal layer;Into step (A1);
(A4) parameter of the regulation device is controlled, object gas release conditions are in using regulation device;It is single in conveying
In the presence of member, gas enters in interlayer from the lower wall of internal layer, the interlayer flowed up to along the interlayer between the wall of side between upper wall
Interior, the object gas of regulation device storage is discharged into gas, is finally entered from the upper wall of internal layer inside internal layer;Into step
(A1);
(A5) terminate;
In above process, if by circulating several times, the target gas content in internal layer still can not reach target
Value, then need to inject foreign gas into the housing by the gas access on housing:As target gas content is all the time in housing
Less than desired value, then inject target gas content and be higher than the gas of desired value, so as to reduce in housing object gas in gas
Content;Target gas content is consistently higher than desired value in such as housing, then injects the gas that target gas content is less than desired value, from
And reduce the content of object gas in gas in housing.
Embodiment 2:
The cell culture incubator of the utility model embodiment, as different from Example 1:
1. regulation device is arranged on the outside of the housing, input and output end are respectively communicated with the double-decker of housing
Interlayer between the wall of side;
2. being additionally provided with supply unit, the gas of interlayer is transported to the regulation device.
Embodiment 3:
Fig. 2 schematically illustrates the structure diagram of the cell culture incubator of the utility model embodiment, as shown in Fig. 2 institute
Stating cell culture incubator includes:
Housing, the housing has gas access, gas vent and humidification port, and the double-deck knot constituted for internal layer, outer layer
Structure;The upper wall and lower wall of the internal layer have through hole;The front side of housing has operation gloves;
Conveyor module, such as blower fan, the conveyor module are arranged on the through hole of the upper wall of the internal layer or the downside of through hole,
For the gas in the internal layer to be transported into regulation device so that gas is in internal layer, interlayer internal circulation flow;
Heating module, the flake heater, the heating module is arranged in the interlayer, such as the inwall of outer layer, side
Portion's interlayer, top interlayer, bottom interlayer have heating module, wherein the heating module in the interlayer of front side is transparent, are easy to see
Internal layer is examined, while improving heating uniformity;
Filter, the filter is arranged on the bottom of the conveyor module, the projection of the filter in the horizontal plane
The figure surrounded with the inwall of the internal layer on the horizontal plane is overlapped;
Regulation device, the regulation device is respectively at the storage of object gas, release according to the difference of running parameter
State, the regulation device is arranged in the top interlayer;The regulation device is 2, is oxygen molecule sieve, carbon dioxide
Molecular sieve, carries out adsorption and desorption, so as to store, discharge object gas using pressure swing adsorption (PSA) to gas molecule;
Oxygen in the internal layer measured, the content of carbon dioxide are sent to processor by sensor, the sensor;
Processor, the processor controls the working condition of the regulation device according to the content received:If less than mesh
Scale value, makes regulation device be in release conditions by control;If higher than desired value, making regulation device be in storage shape by control
State.
The control method of the cell culture incubator of the utility model embodiment, the control method of the cell culture incubator include with
Lower step:
(A1) the target gas such as oxygen, carbon dioxide in gas sensor detection cell culture operations integral system internal layer
The content of body, and it is sent to processor;
(A2) processor goes to control the working condition of regulation device according to the content received:
If desired for the content of reduction object gas, into step (A3);
If desired for the content of rise object gas, into step (A4);
As that need not adjust, into step (A5);
(A3) parameter of the regulation device is controlled, regulation device is in object gas storing state;In conveyor module
In the presence of, gas enters in interlayer from the lower wall of internal layer, is flowed up to along the interlayer between the wall of side in the interlayer between upper wall,
Object gas in gas is adjusted device storage, finally enters from the upper wall of internal layer inside internal layer;Into step (A1);
(A4) parameter of the regulation device is controlled, object gas release conditions are in using regulation device;In conveying mould
In the presence of block, gas enters in interlayer from the lower wall of internal layer, the interlayer flowed up to along the interlayer between the wall of side between upper wall
Interior, the object gas of regulation device storage is discharged into gas, is finally entered from the upper wall of internal layer inside internal layer;Into step
(A1);
(A5) terminate;
In above process, if by circulating several times, the target gas content in internal layer still can not reach target
Value, then need to inject foreign gas into the housing by the gas access on housing:As target gas content is all the time in housing
Less than desired value, then inject target gas content and be higher than the gas of desired value, so as to reduce in housing object gas in gas
Content;Target gas content is consistently higher than desired value in such as housing, then injects the gas that target gas content is less than desired value, from
And reduce the content of object gas in gas in housing;
In the above-mentioned course of work, the gas in heating module heating interlayer so that the temperature in interlayer is higher than in internal layer
Temperature, the inwall of the internal layer do not have condensed water.