CN206223590U - A kind of potato root pressure and transpiration pull measurement system - Google Patents

A kind of potato root pressure and transpiration pull measurement system Download PDF

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CN206223590U
CN206223590U CN201621354922.3U CN201621354922U CN206223590U CN 206223590 U CN206223590 U CN 206223590U CN 201621354922 U CN201621354922 U CN 201621354922U CN 206223590 U CN206223590 U CN 206223590U
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glass tube
transpiration
pressure
valve
plant
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唐勋
司怀军
张宁
付学
李豆豆
冯亚
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Gansu Agricultural University
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Abstract

The utility model provides a kind of potato root pressure and transpiration pull measurement system, using the glass tube that can be sealed, one section of air is sealed with watertight, the other end connects the section of axis, and plant transpiration heat absorbing glass inner air tube volume increase, pressure diminishes, or plant bleeding under the effect of root pressure, inner air tube volume reduces, and pressure becomes big, further according to equation for ideal gases(Clapyron Equation)Calculate transpiration pull or root pressure.The utility model simple structure, low manufacture cost is simple to operate, and the root that can simultaneously measure same plant using two covering devices is pressed and rising pressure, the water suction power total so as to calculate plant, and the measuring system can also realize field measurement in addition.

Description

一种马铃薯根压和蒸腾拉力测定系统A system for measuring potato root pressure and transpiration tension

技术领域technical field

本实用新型涉及一种植物生理学实验装置,具体地说是一种能够测定马铃薯根压和蒸腾拉力的装置。The utility model relates to a plant physiology experiment device, in particular to a device capable of measuring potato root pressure and transpiration pulling force.

背景技术Background technique

蒸腾作用是水分从植物体表面(主要是叶)以水蒸气状态散失到大气中的过程,与物理学的蒸发过程不同,蒸腾作用不仅受外界环境条件的影响,而且还受植物本身的调节和控制,因此它是一种复杂的生理过程。蒸腾作用的生理意义有下列三点:1.蒸腾作用是植物对水分的吸收和运输的一个主要动力,即蒸腾拉力。2.由于矿质盐类要溶于水中才能被植物吸收和在体内运转,蒸腾作用是对水分吸收和流动的动力,因此,矿物质也随水分的吸收和流动而被吸入和分布到植物体各部分中去。3.蒸腾作用能够降低叶片的温度。太阳光照射到叶片上时,大部分能量转变热能,蒸腾作用能降低叶片的温度避免被灼伤。Transpiration is the process in which water is lost from the surface of plants (mainly leaves) to the atmosphere in the form of water vapor. Unlike the evaporation process in physics, transpiration is not only affected by external environmental conditions, but also regulated by the plant itself. control, so it is a complex physiological process. The physiological significance of transpiration has the following three points: 1. Transpiration is a major driving force for plants to absorb and transport water, that is, transpiration pull. 2. Since mineral salts must be dissolved in water to be absorbed by plants and operate in the body, transpiration is the driving force for water absorption and flow, therefore, minerals are also inhaled and distributed to various parts of plants with the absorption and flow of water go. 3. Transpiration reduces the temperature of the leaves. When the sun shines on the leaves, most of the energy is converted into heat energy, and transpiration can reduce the temperature of the leaves to avoid being burned.

蒸腾拉力是由于植物的蒸腾作用而产生一系列水势梯度,使导管中的水分上升的一种力量。当气孔张开后,气孔下腔附近的叶肉细胞因蒸腾失水,水势下降,所以从相邻细胞夺取水分,失水的细胞又从旁边的另一个细胞取得水分,如此下去,从气孔下腔到叶脉导管,再到叶柄、茎的导管,最后到根系导管之间就形成了一系列的水势梯度,最后引起根系从外界土壤环境中吸收水分。这种力量完全是由于叶片的蒸腾作用而形成的,不需要消耗代谢能,因此认为是一种被动吸水,也是植物吸水的主要方式。Transpiration pull is a force that creates a series of water potential gradients due to the transpiration of plants, causing the water in the conduit to rise. When the stomata open, the mesophyll cells near the stomatal cavity lose water due to transpiration, and the water potential drops, so they take water from adjacent cells, and the dehydrated cells get water from another cell next to them, and so on, from the stomatal cavity A series of water potential gradients are formed between the vein ducts, the petiole and stem ducts, and finally the root ducts, which finally cause the roots to absorb water from the external soil environment. This force is completely formed by the transpiration of the leaves, and does not need to consume metabolic energy. Therefore, it is considered to be a passive water absorption, and it is also the main way for plants to absorb water.

根压指的是植物通过消耗能量,通过主动吸收离子,水分随浓度差往上沿木质部运动的生理过程。根压是植物体除蒸腾作用外第二个为水分逆重力流动提供动力的过程。水和溶解其中的离子通过质外体到达根的内皮层,为内皮层细胞间凯氏带所阻,不能自由扩散到内面。内皮细胞上载体蛋白选择性运载离子。离子于是从质外体途径进入到共质体途径。此过程离子是逆浓度运输,植物需消耗ATP以完成这一过程。离子运动的结果,造成内皮层离子浓度高于外面。水分自然会随浓度梯度往中柱流动,进入木质部,被往上引导到植物其他器官。Root pressure refers to the physiological process that plants consume energy and actively absorb ions, and water moves upward along the xylem with the concentration difference. Root pressure is the second process in plants that provides power for water flow against gravity besides transpiration. Water and ions dissolved in it reach the endothelial layer of the root through the apoplast, which is blocked by the Kjeldahl band between the cells of the endothelial layer and cannot freely diffuse to the inner surface. Carrier proteins on endothelial cells selectively transport ions. Ions then pass from the apoplast pathway to the symplast pathway. In this process, ions are transported against the concentration, and plants need to consume ATP to complete this process. As a result of the ion movement, the concentration of ions in the inner cortex is higher than that in the outer layer. Water will naturally flow to the stele along the concentration gradient, enter the xylem, and be guided upward to other organs of the plant.

在湿度较大蒸腾作用很弱的情况下(清晨或夜晚),建立起根压的植物,其木质部的水通过叶缘维管束末端管胞处的排水孔渗出,表现为叶片边缘有水滴存在,这种现象称为吐水。伤流现象指从受伤或折断的植物组织中溢出处液体的一种现象,当横向截断植物的茎,等待两三分钟后,会发现横截面上布满水珠。流出的汁液是伤流液。不同植物伤流程度不同,葫芦科植物伤流液多,稻、麦等的较少。同一植物在不同季节中根系生理活动强弱、根系有效吸收面积大小等都直接影响伤流液的多少。吐水和伤流都说明根压的存在。In the case of high humidity and weak transpiration (early morning or night), the water in the xylem of the plant that has established root pressure seeps out through the drainage holes at the end tracheids of the leaf margin vascular bundles, which is manifested by the presence of water droplets on the leaf edge , this phenomenon is called spit water. Injury flow phenomenon refers to a phenomenon in which liquid overflows from injured or broken plant tissues. When the stem of a plant is cut horizontally and waits for two or three minutes, it will be found that the cross section is covered with water droplets. The sap that comes out is wound fluid. Different plants have different degrees of bleeding, cucurbit plants have more wounding fluid, and rice, wheat, etc. have less. The strength of physiological activities of the root system and the size of the effective absorption area of the root system in the same plant in different seasons all directly affect the amount of wounding fluid. Spitting water and wounding flow both indicate the existence of root pressure.

根压一般较低,大多数植物的根压0.05-0.5MPa,某些木本植物和葡萄的根压则更大一些。在正常情况下,根压对植物所起的作用是有限的,在春季叶片未展开时,蒸腾作用很弱的植株,主动吸水所形成的根压才成为主要吸水动力。一切影响植物根系生理活动的因素都会影响植物根系根压作用的吸水。The root pressure is generally low, the root pressure of most plants is 0.05-0.5MPa, and the root pressure of some woody plants and grapes is greater. Under normal circumstances, the effect of root pressure on plants is limited. When the leaves are not unfolded in spring, the root pressure formed by active water absorption becomes the main water absorption power for plants with weak transpiration. All factors that affect the physiological activities of plant roots will affect the water absorption of plant root root pressure.

综上所述,植物根压和蒸腾拉力是植物吸收水分的两大主要动力,因此在植物水分吸收,抗旱性,节水灌溉等领域的研究中需要测量根压和蒸腾拉力。而现有技术中未见方便,灵敏的测量仪器。To sum up, plant root pressure and transpiration pull are the two main driving forces for plants to absorb water. Therefore, it is necessary to measure root pressure and transpiration pull in the fields of plant water absorption, drought resistance, and water-saving irrigation. And there is no convenience in the prior art, sensitive measuring instrument.

发明内容Contents of the invention

鉴于现有技术的不足,本实用新型提供了一种马铃薯根压和蒸腾拉力测定系统,采用能够密封的玻璃管,用水密封住一段空气,另一端连接植物茎的断面。植物蒸腾吸收玻璃管内空气体积增大,压强变小,或植物在根压作用下伤流,管内空气体积减小,压强变大,根据理想气体方程(克拉伯龙方程)计算出蒸腾拉力或根压。In view of the deficiencies in the prior art, the utility model provides a potato root pressure and transpiration tension measuring system, which adopts a glass tube that can be sealed, seals a section of air with water, and connects the section of the plant stem at the other end. Plant transpiration absorbs the air volume in the glass tube to increase and the pressure to decrease, or the plant is wounded under the root pressure, the air volume in the tube decreases and the pressure increases, and the transpiration pull or root pressure is calculated according to the ideal gas equation (Clapeyron equation). pressure.

为了达到上述目的,本实用新型采用了如下的技术方案:In order to achieve the above object, the utility model adopts the following technical solutions:

一种马铃薯根压和蒸腾拉力测定系统,其包括:固定机构和测定装置,所述固定机构包括:测量台、茎固定器、水平仪;所述测定装置包括:玻璃管、阀门1、阀门2、阀门3、阀门4、储水箱、温度计、橡胶管、橡皮套。茎固定器、水平仪和测定装置都放置在测量台上;橡皮套、阀门3、橡胶管、阀门4、储水箱、阀门1、玻璃管、阀门2依次连接。A potato root pressure and transpiration tension measuring system, comprising: a fixing mechanism and a measuring device, the fixing mechanism comprising: a measuring platform, a stem fixer, a level; the measuring device comprising: a glass tube, a valve 1, a valve 2, Valve 3, valve 4, water storage tank, thermometer, rubber tube, rubber sleeve. Stem fixer, level meter and measuring device are all placed on the measuring table; rubber sleeve, valve 3, rubber tube, valve 4, water storage tank, valve 1, glass tube, valve 2 are connected in sequence.

优选地,所述玻璃管上有均匀的刻度,能读出气体体积大小,玻璃管的内径根据水的表面张力能够使水在玻璃管内形成水柱,经计算内径为0.1-50mm为宜。Preferably, there is a uniform scale on the glass tube, which can read the volume of the gas. The inner diameter of the glass tube can make the water form a water column in the glass tube according to the surface tension of the water. The calculated inner diameter is preferably 0.1-50mm.

优选地,所述测量台为一折叠小桌,桌腿能够伸缩以调节高度。Preferably, the measuring table is a folding small table, and the legs of the table can be stretched to adjust the height.

优选地,所述橡皮套为弹性橡胶制成,能够密封茎的断面。Preferably, the rubber sheath is made of elastic rubber, capable of sealing the section of the stem.

优选地,所述阀门3的出口能够连接注射器。Preferably, the outlet of the valve 3 can be connected with a syringe.

优选地,所述茎固定器为金属支架,通过螺栓固定切下的植物枝条。Preferably, the stem holder is a metal bracket, and the cut plant branches are fixed by bolts.

优选地,所述温度计放置在玻璃管旁边,测量实验温度。Preferably, the thermometer is placed next to the glass tube to measure the experimental temperature.

优选地,所述玻璃管还可以在其一端设有小孔,能将温度计套一橡皮环再塞入小孔,直接测量玻璃管内气体温度。Preferably, the glass tube can also be provided with a small hole at one end thereof, and a rubber ring can be inserted into the small hole with a thermometer covered to directly measure the temperature of the gas in the glass tube.

优选地,所述玻璃管还可以在其一端的内侧安装温度传感器,利用导线穿过玻璃管壁,连接电子温度计测量管内空气温度。Preferably, a temperature sensor can also be installed inside one end of the glass tube, and a wire can be used to pass through the wall of the glass tube and connected to an electronic thermometer to measure the temperature of the air in the tube.

优选地,所述玻璃管还可以放置在恒温箱内,使玻璃管温度恒定。Preferably, the glass tube can also be placed in a constant temperature box to keep the temperature of the glass tube constant.

一种马铃薯根压和蒸腾拉力测定方法,包括以下操作步骤:A method for measuring potato root pressure and transpiration tension, comprising the following steps:

(1)实验准备:将上述植物根压和蒸腾拉力测定系统带到温室或者田间,调节测量台高度,使之与将要截取的茎截面相当。(1) Experiment preparation: Take the above-mentioned plant root pressure and transpiration tension measurement system to the greenhouse or field, and adjust the height of the measuring platform to make it equivalent to the section of the stem to be cut.

(2)玻璃管注水:手持玻璃管阀门2一端,使玻璃管竖直,用细绳扎住橡皮套,再用注射器连接阀门3,打开阀门2,用注射器往玻璃管内注水直到水面上升到玻璃管2/3处停止,同时关闭阀门2。(2) Glass tube water injection: hold one end of the glass tube valve 2, make the glass tube vertical, tie the rubber sheath with a string, connect the valve 3 with a syringe, open the valve 2, and fill the glass tube with water until the water surface rises to the glass tube. Pipe 2/3 stops, and valve 2 is closed at the same time.

(3)连接植物茎断面:轻轻截断待测植物的茎,使断面平整,将橡皮套套在断面上,再用封口膜缠绕数周,使之密封良好;解开扎橡皮套的细绳;橡皮套与植物断面接触处充满水。(3) Connect the section of the plant stem: Gently cut off the stem of the plant to be tested to make the section flat, put a rubber sleeve on the section, and then wrap it with a parafilm for several weeks to make it well sealed; untie the string tied with the rubber sleeve; The contact between the rubber sleeve and the plant section is filled with water.

(4)观察:将玻璃管平放与植物断面等高,观察并记录气体体积和气体温度,在蒸腾拉力作用下,植物蒸腾消耗水,气体体积扩大;在根压作用下,植物茎断面伤流,玻璃管内水增多,气体体积变小;直到平衡时(气体体积不再变化)记录气体体积和温度。(4) Observation: Place the glass tube flat on the same height as the plant section, observe and record the gas volume and gas temperature, under the action of transpiration tension, the plant consumes water through transpiration, and the gas volume expands; under the action of root pressure, the plant stem section is injured Flow, the water in the glass tube increases, and the volume of the gas decreases; until the equilibrium (the volume of the gas does not change), record the volume and temperature of the gas.

(5)计算:测量前玻璃管内空气为大气气压,根据实验当地海拔可知;玻璃管内气体满足理想气体的条件,并且玻璃管内气体物质的量不发生变化,因此,根据前后两次体积和温度(可将玻璃管放在恒温箱内,使温度不变)即可计算出测量前后玻璃管内气体的压强,压强差即为根压和蒸腾拉力,计算公式如下:(5) Calculation: The air in the glass tube is atmospheric pressure before measurement, which can be known according to the local altitude of the experiment; the gas in the glass tube meets the conditions of an ideal gas, and the amount of gas substances in the glass tube does not change. Therefore, according to the volume and temperature ( The glass tube can be placed in a constant temperature box to keep the temperature constant) to calculate the pressure of the gas in the glass tube before and after the measurement. The pressure difference is the root pressure and the transpiration pull. The calculation formula is as follows:

PV=nRTPV=nRT

其中,in,

P—为玻璃管内气体气压,单位为Pa;P - is the gas pressure in the glass tube, the unit is Pa;

V—为玻璃管内气体体积,单位为m3 V—is the volume of gas in the glass tube, in m3

n—为玻璃管内气体物质的量,单位为mol;n—is the amount of gas substance in the glass tube, the unit is mol;

R—为常量,数值和单位为8.31Pa·m3·mol-1·K-1 R—is a constant, the value and unit are 8.31Pa·m 3 ·mol -1 ·K -1

T—为玻璃管内气体温度,单位为K。T—is the gas temperature in the glass tube, in K.

有益效果:本实用新型结构简单,制作成本低,操作简单,采用两套装置即能同时测量同一植株的根压和蒸腾压力,从而计算出植物总的吸水动力,此外该测量系统还能够实现田间测量。Beneficial effects: the utility model has the advantages of simple structure, low production cost and simple operation. Two sets of devices can be used to simultaneously measure the root pressure and transpiration pressure of the same plant, thereby calculating the total water absorption power of the plant. In addition, the measurement system can also realize field Measurement.

附图说明Description of drawings

图1是本实用新型一种马铃薯根压和蒸腾拉力测定系统的结构示意图;Fig. 1 is the structural representation of a kind of potato root pressure of the present utility model and transpiration tension measuring system;

图2是本实用新型一种马铃薯根压和蒸腾拉力测定系统温度计连接示意图;Fig. 2 is a kind of potato root pressure of the utility model and transpiration pulling force measuring system thermometer connection schematic diagram;

图3是本实用新型一种马铃薯根压和蒸腾拉力测定系统温度传感器连接示意图;Fig. 3 is a kind of potato root pressure of the utility model and transpiration tension measurement system temperature sensor connection schematic diagram;

图4是本实用新型一种马铃薯根压和蒸腾拉力测定系统测定根压示意图;Fig. 4 is that a kind of potato root pressure of the utility model and transpiration tension measuring system measure root pressure schematic diagram;

图5是本实用新型一种马铃薯根压和蒸腾拉力测定系统测定蒸腾拉力示意图。Fig. 5 is a schematic diagram of measuring transpiration pulling force by a potato root pressure and transpiration pulling force measuring system of the present invention.

具体实施方式detailed description

为使实用新型的目的、技术方案和优点更加清楚,下面结合附图对本实用新型的具体实施方式进行详细说明。这些优选实施方式的示例在附图中进行了例示。附图中所示和根据附图描述的本实用新型的实施方式仅仅是示例性的,并且本实用新型并不限于这些实施方式。In order to make the purpose, technical solution and advantages of the utility model clearer, the specific implementation of the utility model will be described in detail below in conjunction with the accompanying drawings. Examples of these preferred embodiments are illustrated in the accompanying drawings. The embodiments of the invention shown in the drawings and described with reference to the drawings are merely exemplary, and the invention is not limited to these embodiments.

在此,还需要说明的是,为了避免因不必要的细节而模糊了本实用新型,在附图中仅仅示出了与根据本实用新型的方案密切相关的结构和/或处理步骤,而省略了关系不大的其他细节。Here, it should also be noted that, in order to avoid obscuring the utility model due to unnecessary details, only the structures and/or processing steps closely related to the solution according to the utility model are shown in the drawings, and the other minor details.

实施例1Example 1

本实施例提供了一种马铃薯根压和蒸腾拉力测定系统,如图1所示,其包括:固定机构和测定装置,所述固定机构包括:测量台9、茎固定器7、水平仪10;所述测定装置包括:玻璃管12、阀门1、阀门2、阀门3、阀门4、储水箱5、温度计8、橡胶管11、橡皮套6。茎固定器7、水平仪10和测定装置都放置在测量台上;橡皮套6、阀门3、橡胶管11、阀门4、储水箱5、阀门1、玻璃管12、阀门2依次连接。The present embodiment provides a kind of potato root pressure and transpiration tension measurement system, as shown in Figure 1, it comprises: fixing mechanism and measuring device, and described fixing mechanism comprises: measuring table 9, stem holder 7, level 10; The measuring device includes: a glass tube 12, a valve 1, a valve 2, a valve 3, a valve 4, a water storage tank 5, a thermometer 8, a rubber tube 11, and a rubber sleeve 6. Stem fixer 7, spirit level 10 and measuring device are all placed on the measuring platform; Rubber sleeve 6, valve 3, rubber tube 11, valve 4, water storage tank 5, valve 1, glass tube 12, valve 2 are connected successively.

进一步地,所述玻璃管12上有均匀的刻度,能读出气体体积大小,玻璃管12的内径根据水的表面张力能够使水在玻璃管12内形成水柱,经计算内径为0.1-50mm为宜。Further, there is a uniform scale on the glass tube 12, which can read the volume of the gas. The inner diameter of the glass tube 12 can make water form a water column in the glass tube 12 according to the surface tension of the water. After calculation, the inner diameter is 0.1-50mm. should.

进一步地,所述测量台9为一折叠小桌,桌腿能够伸缩以调节高度。Further, the measuring table 9 is a foldable small table, and the legs of the table can be stretched to adjust the height.

进一步地,所述橡皮套6为弹性橡胶制成,能够密封茎的断面。Further, the rubber sheath 6 is made of elastic rubber, which can seal the section of the stem.

进一步地,所述阀门3的出口能够连接注射器。Further, the outlet of the valve 3 can be connected with a syringe.

进一步地,所述茎固定器7为金属支架,通过螺栓固定切下的植物枝条。Further, the stem fixer 7 is a metal bracket, and the cut plant branches are fixed by bolts.

进一步地,所述温度计8放置在玻璃管12旁边,测量实验温度。Further, the thermometer 8 is placed next to the glass tube 12 to measure the experimental temperature.

进一步地,所述玻璃管12还可以放置在恒温箱内,使玻璃管12温度恒定。Further, the glass tube 12 can also be placed in a constant temperature box to keep the temperature of the glass tube 12 constant.

如附图2,所述玻璃管还可以在其一端设有小孔,能将温度计8套一橡皮环塞入小孔,直接测量玻璃管8内气体温度。As accompanying drawing 2, described glass tube can also be provided with aperture at its one end, can insert a rubber ring of 8 sets of thermometers into aperture, directly measure the gas temperature in glass tube 8.

如附图3,所述玻璃管还可以在其一端的内侧安装温度传感器13,利用导线穿过玻璃管壁,连接电子温度计14测量管内空气温度。As accompanying drawing 3, described glass tube can also install temperature sensor 13 in the inboard of its one end, utilize wire to pass glass tube wall, connect electronic thermometer 14 to measure the air temperature in the tube.

实施例2Example 2

本实施例提供了一种马铃薯根压和蒸腾拉力测定方法,如图4和5所示,包括以下操作步骤:Present embodiment provides a kind of potato root pressure and transpiration pulling force measuring method, as shown in Figure 4 and 5, comprises the following steps:

(1)实验准备:将上述植物根压和蒸腾拉力测定系统带到温室或者田间,调节测量台9高度,使之与将要截取的茎截面相当。(1) Experiment preparation: Take the above-mentioned plant root pressure and transpiration tension measurement system to the greenhouse or field, and adjust the height of the measuring platform 9 to be equivalent to the section of the stem to be cut.

(2)玻璃管注水:手持玻璃管阀门2一端,使玻璃管9竖直,用细绳扎住橡皮套6,再用注射器连接阀门3,打开阀门2,用注射器往玻璃管9内注水直到水面上升到玻璃管2/3处停止,同时关闭阀门2。(2) Glass tube water injection: hold one end of the glass tube valve 2, make the glass tube 9 vertical, tie the rubber sheath 6 with a string, connect the valve 3 with a syringe, open the valve 2, and fill the glass tube 9 with water until The water surface rises to the glass tube 2/3 and stops, and the valve 2 is closed at the same time.

(3)连接植物茎断面:轻轻截断待测植物的茎,使断面平整,将橡皮套6套在断面上,再用封口膜缠绕数周,使之密封良好;解开扎橡皮套6的细绳;橡皮套6与植物断面接触处充满水。(3) Connect the section of the plant stem: Gently cut off the stem of the plant to be tested to make the section flat, put the rubber sleeve 6 on the section, and wrap it with a parafilm for several weeks to make it well sealed; untie the rubber sleeve 6 String; Rubber sheath 6 is full of water with plant cross-section contact place.

(4)观察:将玻璃管12平放与植物断面等高,观察并记录气体体积和气体温度,在蒸腾拉力作用下,植物蒸腾消耗水,气体体积扩大;在根压作用下,植物茎断面伤流,玻璃管12内水增多,气体体积变小;直到平衡时(气体体积不再变化)记录气体体积和温度。(4) Observation: place the glass tube 12 flat on the same height as the plant section, observe and record the gas volume and gas temperature, under the action of transpiration tension, the plant consumes water through transpiration, and the gas volume expands; under the action of root pressure, the plant stem section Injured flow, the water in the glass tube 12 increases, and the gas volume decreases; until the equilibrium (gas volume no longer changes), record the gas volume and temperature.

(5)计算:测量前玻璃管12内空气为大气气压,根据实验当地海拔可知;玻璃管12内气体满足理想气体的条件,并且玻璃管12内气体物质的量不发生变化,因此,根据前后两次体积和温度(可将玻璃管放在恒温箱内,使温度不变)即可计算出测量前后玻璃管12内气体的压强,压强差即为根压和蒸腾拉力,计算公式如下:(5) Calculation: The air in the glass tube 12 before measurement is atmospheric pressure, which can be known according to the local altitude of the experiment; the gas in the glass tube 12 satisfies the conditions of an ideal gas, and the amount of the gas substance in the glass tube 12 does not change. Therefore, according to the before and after Two times of volume and temperature (the glass tube can be placed in a constant temperature box to keep the temperature constant) can be used to calculate the pressure of the gas in the glass tube 12 before and after the measurement. The pressure difference is the root pressure and the transpiration pull. The calculation formula is as follows:

PV=nRTPV=nRT

其中,in,

P—为玻璃管内气体气压,单位为Pa;P - is the gas pressure in the glass tube, the unit is Pa;

V—为玻璃管内气体体积,单位为m3 V—is the volume of gas in the glass tube, in m3

n—为玻璃管内气体物质的量,单位为mol;n—is the amount of gas substance in the glass tube, the unit is mol;

R—为常量,数值和单位为8.31Pa·m3·mol-1·K-1 R—is a constant, the value and unit are 8.31Pa·m 3 ·mol -1 ·K -1

T—为玻璃管内气体温度,单位为K。T—is the gas temperature in the glass tube, in K.

综上所述,本实用新型提供了一种马铃薯根压和蒸腾拉力测定系统,采用能够密封的玻璃管,用水密封住一段空气,另一端连接植物茎的断面。植物蒸腾吸收玻璃管内空气体积增大,压强变小,或植物在根压作用下伤流,管内空气体积减小,压强变大,根据理想气体方程(克拉伯龙方程)计算出蒸腾拉力或根压。In summary, the utility model provides a measurement system for potato root pressure and transpiration tension, which adopts a glass tube that can be sealed, seals a section of air with water, and connects the section of the plant stem at the other end. Plant transpiration absorbs the air volume in the glass tube to increase and the pressure to decrease, or the plant is wounded under the root pressure, the air volume in the tube decreases and the pressure increases, and the transpiration pull or root pressure is calculated according to the ideal gas equation (Clapeyron equation). pressure.

以上所述仅是本申请的具体实施方式,应当指出,对于本技术领域的普通技术人员来说,在不脱离本申请原理的前提下,还可以做出若干改进和润饰,这些改进和润饰也应视为本申请的保护范围。The above description is only the specific implementation of the present application. It should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present application, some improvements and modifications can also be made. It should be regarded as the protection scope of this application.

Claims (5)

1. A potato root pressure and transpiration tension measuring system is characterized by comprising: a fixing mechanism and an assay device, the fixing mechanism comprising: a measuring table, a stem fixer and a level meter; the assay device comprises: the device comprises a glass tube, a valve 1, a valve 2, a valve 3, a valve 4, a water storage tank, a thermometer, a rubber tube and a rubber sleeve; the stem fixator, the gradienter and the measuring device are all arranged on the measuring table; the rubber sleeve, the valve 3, the rubber tube, the valve 4, the water storage tank, the valve 1, the glass tube and the valve 2 are connected in sequence.
2. The potato root pressure and transpiration tension measuring system according to claim 1, wherein an outlet of the valve 3 is connectable to a syringe.
3. The potato root pressure and transpiration tension measuring system according to claim 1, wherein the glass tube may be provided with a small hole at one end thereof, and a thermometer sleeve and a rubber ring can be inserted into the small hole to directly measure the temperature of the gas in the glass tube.
4. The potato root pressure and transpiration tension measuring system according to claim 1, wherein the glass tube is further provided with a temperature sensor at an inner side of one end thereof, and a lead wire is passed through the wall of the glass tube and connected with an electronic thermometer to measure the temperature of air in the tube.
5. The potato root pressure and transpiration tension determination system of claim 1, wherein the glass tube is placed in an incubator so that the temperature of the glass tube is constant.
CN201621354922.3U 2016-12-12 2016-12-12 A kind of potato root pressure and transpiration pull measurement system Expired - Fee Related CN206223590U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106483038A (en) * 2016-12-12 2017-03-08 甘肃农业大学 Plant roots pressure and transpiration pull analyzer and its assay method
CN107493780A (en) * 2017-09-26 2017-12-22 遵义百草谷药业有限公司 A kind of implantation methods of the fleece-flower root

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106483038A (en) * 2016-12-12 2017-03-08 甘肃农业大学 Plant roots pressure and transpiration pull analyzer and its assay method
CN107493780A (en) * 2017-09-26 2017-12-22 遵义百草谷药业有限公司 A kind of implantation methods of the fleece-flower root

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