CN206204286U - Liquid transmission assembly and the gene sequencer of the liquid transmission assembly is installed - Google Patents

Abstract

The utility model provides a kind of liquid transmission assembly and is provided with the gene sequencer of the liquid transmission assembly, is related to technical field of biological.Gene sequencer includes control system, power module and liquid transmission assembly, control system is electrically connected with power module, liquid transmission assembly, liquid transmission assembly includes liquid transfer gun head, gene sequencing box, sewer pipe and drawdown pump, liquid transfer gun head is connected with gene sequencing box, gene sequencing box is connected by sewer pipe with drawdown pump, gene sequencing box includes housing and dividing plate, housing is provided with accommodating chamber, dividing plate simultaneously forms interlayer with housing, genetic chip is provided with accommodating chamber, temperature sensor and Peltier is provided with interlayer and the two is electrically connected with control system respectively.The liquid transmission assembly low cost, is capable of achieving rapid, accurate transition temperature.The gene sequencer can fast and accurately obtain the result of gene sequencing.

Description

Liquid transmission assembly and the gene sequencer of the liquid transmission assembly is installed

Technical field

The utility model is related to technical field of biological, in particular to a kind of liquid transmission assembly and is provided with The gene sequencer of the liquid transmission assembly.

Background technology

Gene sequencing technology is just turning into the science and technology for promoting human society fast development in this century, and gene sequencing technology is Principle according to base pair complementarity detects the nucleotide sequence of life entity.The liquid transmission assembly of current tradition gene sequencing is all It is by placing gene sequencing chip and gene sequencing reagent in tester, carrying out physics to gene sequencing reagent anti- The detection to gene sequencing reagent should be finally realized with the operation of chemical reaction, and it is anti-in the physical chemistry of gene sequencing reagent Should during, it is necessary to the operation lowered the temperature to sample or heated up, and these coolings, the operating process that heats up must be rapid, The time is not only otherwise expended, and is likely to result in gene sequencing reagent and gone bad so that testing result is inaccurate.In addition, having very Many materials are also different in the temperature needed for the differential responses stage, it is therefore desirable to the said equipment instrument can rapidly change temperature Degree.And attemperating units most in the prior art all can not simultaneously meet rapid, accurate cooling and warming and rapid transformation temperature The requirement of degree.Although having a small number of attemperating units can reach above-mentioned requirements, their manufacturing cost is high, use cost is high It is high, it is unfavorable for that popularization is used.

Utility model content

The purpose of this utility model is to provide a kind of liquid transmission assembly, and its low cost quick and precisely obtains gene survey Temperature in sequence box, so as to realize rapid, accurate cooling and warming and rapid transition temperature.

Another object of the present utility model is to provide a kind of gene sequencer, and it can fast and accurately obtain gene The result of sequencing.

What embodiment of the present utility model was realized in：

A kind of liquid transmission assembly, is adapted to be mounted to gene sequencer, and gene sequencer includes control system, power module With liquid transmission assembly, control system is electrically connected with power module, liquid transmission assembly, and liquid transmission assembly includes liquid relief Pipette tips, gene sequencing box, sewer pipe and drawdown pump, are used to accommodate gene sequencing reagent, liquid transfer gun head and gene in liquid transfer gun head Sequencing box connection, gene sequencing box is connected by sewer pipe with drawdown pump, and gene sequencing box includes housing and dividing plate, and housing sets There is accommodating chamber, dividing plate is arranged at housing and interlayer is formed between housing, and genetic chip is provided with accommodating chamber, is provided with interlayer Temperature sensor and Peltier, temperature sensor are electrically connected with control system respectively with Peltier.

In the utility model preferred embodiment, aforementioned barriers include the first dividing plate, and the first dividing plate is arranged at gene survey The bottom of sequence box, temperature sensor is arranged at housing.

In the utility model preferred embodiment, above-mentioned housing is provided with fin away from the side of accommodating chamber, positioned at fin The thickness of the housing in region for surrounding is less than the thickness of the housing outside the region that fin is surrounded, and temperature sensor interference is embedding Located at fin.

In the utility model preferred embodiment, aforementioned barriers include second partition, second partition and gene sequencing box At least one side wall form the second interlayer, Peltier is arranged in the second interlayer, and the position of second partition correspondence Peltier sets It is equipped with louvre.

In the utility model preferred embodiment, above-mentioned Peltier is arranged at the outer wall of housing.

In the utility model preferred embodiment, the thickness of the thickness less than the second interlayer of above-mentioned Peltier.

In the utility model preferred embodiment, said temperature sensor is infrared temperature-test sensor.

Above-mentioned also to include surge flask in the utility model preferred embodiment, surge flask is connected to decompression by pipeline Between pump and sewer pipe.

Above-mentioned also to include controlled valve in the utility model preferred embodiment, controlled valve is arranged at sewer pipe.

A kind of gene sequencer, it is provided with aforesaid liquid transmission assembly.

Liquid transmission assembly and the gene sequencer of the liquid transmission assembly is installed that the utility model embodiment is provided Beneficial effect be：Temperature sensor is set in interlayer by gene sequencing box, is surveyed such that it is able to quick and precisely obtain gene Temperature in sequence box, controls Peltier to freeze or heat, so as to quickly carry out temperature in gene sequencing box by control system Regulation, makes testing result more accurate.

Brief description of the drawings

In order to illustrate more clearly of the technical scheme of the utility model embodiment, below will be to be used needed for embodiment Accompanying drawing be briefly described, it will be appreciated that the following drawings illustrate only some embodiments of the present utility model, therefore should not be by Regard the restriction to scope as, for those of ordinary skill in the art, on the premise of not paying creative work, may be used also Other related accompanying drawings are obtained with according to these accompanying drawings.

The structural representation of the liquid transmission assembly that Fig. 1 is provided for the utility model embodiment；

The structural representation of the gene sequencing box that Fig. 2 is provided for the utility model embodiment；

Fig. 3 is close-up schematic view at III in Fig. 2；

Fig. 4 is close-up schematic view at IV in Fig. 2.

Icon：200- liquid transmission assemblies；210- gene sequencing boxes；211- housings；212- accommodating chambers；213- gene cores Piece；214- fins；220- caps；230- dividing plates；231- interlayers；The dividing plates of 232- first；The interlayers of 233- first；234- second every Plate；The interlayers of 235- second；236- louvres；240- temperature sensors；250- Peltiers；260- liquid transfer gun heads；270- sewer pipes； 271- controlled valves；280- drawdown pumps；290- surge flasks.

Specific embodiment

It is new below in conjunction with this practicality to make the purpose, technical scheme and advantage of the utility model embodiment clearer Accompanying drawing in type embodiment, is clearly and completely described, it is clear that retouched to the technical scheme in the utility model embodiment The embodiment stated is a part of embodiment of the utility model, rather than whole embodiments.Generally described in accompanying drawing herein and The component of the utility model embodiment for showing can be arranged and designed with a variety of configurations.

Therefore, the detailed description of embodiment of the present utility model below to providing in the accompanying drawings is not intended to limit requirement The scope of the present utility model of protection, but it is merely representative of selected embodiment of the present utility model.Based in the utility model Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all Belong to the scope of the utility model protection.

It should be noted that：Similar label and letter represents similar terms in following accompanying drawing, therefore, once a certain Xiang Yi It is defined in individual accompanying drawing, then it need not be further defined and explained in subsequent accompanying drawing.

, it is necessary to explanation, term " " center ", " on ", D score " interior ", " outward " etc. are indicated in description of the present utility model Orientation or position relationship be that, based on orientation shown in the drawings or position relationship, or the utility model product is usual when using The orientation or position relationship put, are for only for ease of and describe the utility model and simplify to describe, rather than instruction or hint institute The device or element of finger must have specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to this reality With new limitation.Additionally, term " first ", " second " etc. are only used for distinguishing description, and it is not intended that indicating or implying phase To importance.

Additionally, the term such as term " level ", " vertical " is not offered as requiring part abswolute level or pendency, and can be slightly Low dip.It is not that the expression structure must be complete if " level " refers to only its direction with respect to more level for " vertical " Full level, and can be to be slightly tilted.

In description of the present utility model, in addition it is also necessary to explanation, unless otherwise clearly defined and limited, term " sets Put ", " installation ", " connection " should be interpreted broadly, for example, it may be fixedly connected, or be detachably connected, or integratedly Connection；Can mechanically connect, or electrically connect；Can be joined directly together, it is also possible to be indirectly connected to by intermediary, Can be two connections of element internal.For the ordinary skill in the art, above-mentioned art can be understood with concrete condition Concrete meaning of the language in the utility model.

The present embodiment provides a kind of gene sequencer (not shown), and it includes control system (not shown), power module (figure Do not show) and liquid transmission assembly 200, wherein, control system is electrically connected with power module, liquid transmission assembly 200.

Fig. 1 is referred to, liquid transmission assembly 200 includes gene sequencing box 210, liquid transfer gun head 260, sewer pipe 270, decompression Pump 280 and surge flask 290.

Also referring to Fig. 2, Fig. 3 and Fig. 4, wherein gene sequencing box 210 include housing 211, cap 220, dividing plate 230, Temperature sensor 240 and Peltier 250.

Referring to has accommodating chamber 212 in Fig. 2, housing 211, it is preferable that in the present embodiment, the cross section of housing 211 is " u "-shaped, the housing 211 and accommodating chamber 212 are substantially rectangular in the projection of horizontal plane, its each corner angle through corners at Reason, is easy to the later stage to clean.It is horizontally disposed in accommodating chamber 212 to have genetic chip 213 and accommodate reaction solution (not shown), base Because chip 213 is used to be sequenced, reaction solution is used to protect the genetic chip 213.

Cap 220 is arranged at the top of housing 211, and with the opening of housing 211 at interference fit, accommodated for sealing Chamber 212.Cap 220 offers the first opening (not shown) with the second opening (not shown).

Dividing plate 230 is arranged at housing 211 and interlayer 231 is formed between housing 211.Specifically, dividing plate 230 includes first Dividing plate 232 and second partition 234, interlayer 231 include the first interlayer 233 and the second interlayer 235.

Preferably, Fig. 3 is referred to, the first dividing plate 232 is arranged at the bottom of housing 211, the first dividing plate 232 and housing 211 Between form the first interlayer 233, the thickness of the thickness more than housing 211 of the first dividing plate 232.

Second partition 234 forms the second interlayer 235 with least one side wall of housing 211；Such as second partition 234 and shell The relative two side wall of body 211 forms second interlayer 235 etc., in the present embodiment, preferably second partition 234 and gene sequencing Four side walls of box 210 are respectively formed the second interlayer 235, that is to say that the second interlayer 235 encloses the side wall located at gene sequencing box 210, And first do not contact between dividing plate 232 and second partition 234.Preferably, the top end face of the second interlayer 235 and housing 211 it Between distance more than the distance between the second interlayer 235 and bottom end face of housing 211.

Temperature sensor 240, is the rule varied with temperature using the various physical properties of material, is electricity temperature transition Sensor.Preferably, please continue to refer to Fig. 3, temperature sensor 240 is arranged in the first interlayer 233 and is installed on housing 211 On, it is easy to faster obtain the situation of change of temperature.Specifically, housing 211 is provided with along vertical away from the side of accommodating chamber 212 The fin 214 that direction extends downwardly, it is preferable that the fin 214 is located at the center of the bottom end face of housing 211, and positioned at fin The thickness of the housing 211 in 214 regions for surrounding is less than the thickness of the housing 211 outside the region surrounded positioned at fin 214, and position The material in the region surrounded in fin 214 is heat conducting material, such that it is able to more rapidly be accurately obtained in gene sequencing box 210 Temperature, the thickness of the housing 211 being preferably placed in the region that fin 214 is surrounded is uniform, so that temperature sensor 240 is received Hot uniform, the temperature of measurement is more accurate.The preferred interference of temperature sensor 240 is embedded at fin 214, so as to by temperature sensor 240 while be firmly fixed to 211 surface of housing, the problems such as the fixation of the initiation that prevents from expanding with heat and contract with cold is insecure.

Temperature sensor 240 is electrically connected with control system.Preferably, temperature sensor 240 is infrared temperature-test sensor, its It is cheap, it is preferable that the position of the correspondence infrared temperature-test sensor of the first dividing plate 232 is provided with signal launch hole (not shown), Signal is transmitted to control system through signal launch hole.

Peltier 250 is the cooling and warming element made based on peltier effect.Peltier effect refers to DC current warp During two kinds of metallic conductors being connected, its junction will produce the temperature difference, i.e. junction to produce heat absorption and exothermic phenomenon.Peltier is imitated Should in caloric receptivity and thermal discharge number determined by the size of electric current.The Peltier 250 that the utility model is provided refers to Peltier Heating and cooling chip, it is simultaneously absorbed heat (refrigeration) when being powered, and another side heat release (is heated), can be by changing sense of current Change heat-absorbent surface and heat delivery surface.Because Peltier heating and cooling chip belongs to prior art, therefore herein will no longer to its specific knot Structure and manufacture method etc. are described in detail.

Preferably, Fig. 4 is referred to, Peltier 250 is arranged in the second interlayer 235 and is connected with housing 211.Preferably, Less than the thickness of the second interlayer 235, the thickness of Peltier 250 described herein refers to it away from housing to the thickness of Peltier 250 To the distance near the side of housing 211, the thickness of the second interlayer 235 described herein refers to the outer wall of housing 211 for 211 side To second partition 234 near the distance between side of the outer wall of housing 211.It is highly preferred that the quantity of Peltier 250 is two pieces, The relative both sides of housing 211 are respectively arranged at, so that be heated in accommodating chamber 212 or caught a cold uniform.The corresponding handkerchief of second partition 234 You are provided with louvre 236 in the position of patch 250, wherein, louvre 236 is evenly distributed in second partition 234, so as to prevent handkerchief The problem of your 250 worked thermal initiations of patch, and the state of the size, shape and distribution of louvre 236, this area staff Can specifically be set according to actual conditions, be belonged to the scope of the utility model protection.

Two equal control systems of Peltier 250 electrical connection, so that when temperature sensor 240 is by the temperature in accommodating chamber 212 Transmit to control system, control system judgement or the rise or decline of manual control temperature, and Paar is controlled by control system The heating of patch 250 or refrigeration, thus precisely, the step of quickly realize to being heated up or being lowered the temperature in gene sequencing box 210.

Liquid transfer gun head 260 is connected by the first opening with accommodating chamber 212, for gene sequencing reagent to be added into gene sequencing Box 210.

Please continue to refer to Fig. 1, sewer pipe 270 is connected by the second opening with accommodating chamber 212, for by gene sequencing box Waste reaction solution outflow in 210, is that gene sequencing box 210 can keep gene sequencing reaction.Sewer pipe 270 is provided with and connects with it Logical controlled valve 271, the air pressure break-make of sewer pipe 270 can be controlled using controlled valve 271, and then control gene sequencing box The flowing break-make of gene sequencing reagent in 210.Preferably, controlled valve 271 is check valve, and it includes relative liquid feeding end and goes out Liquid end, wherein liquid feeding end connect with sewer pipe 270 near one end of gene sequencing box 210, and outlet end is with sewer pipe 270 near slow Rush one end connection of bottle 290.

Drawdown pump 280 is arranged on sewer pipe 270 and is connected with sewer pipe 270, and it passes through sewer pipe 270 and extracts gene survey Air in sequence box 210, and then reduce the air pressure in gene sequencing box 210, and then by liquid relief under the actuating pressure of atmospheric pressure In gene sequencing reagent press-in gene sequencing box 210 in pipette tips 260, it is acquired for gene sequencing chip.

Surge flask 290 is connected between drawdown pump 280 and sewer pipe 270 by pipeline, so as to prevent gene sequencing box 210 Interior gene sequencing reagent enters drawdown pump 280, and then prevents drawdown pump 280 to be damaged.

The working condition of liquid transmission assembly 200 and the gene sequencer for being provided with the liquid transmission assembly 200 is：Liquid relief Pipette tips 260 add gene sequencing reagent in gene sequencing box 210, are acquired for gene sequencing chip, in gatherer process, temperature The temperature in accommodating chamber 212 that degree sensor 240 will constantly be measured is transmitted to air strike system, control system control Peltier 250 heating are lowered the temperature, and reaction is finished, and waste liquid flows out through sewer pipe 270.

In sum, the utility model embodiment is provided liquid transmission assembly 200 and the liquid transmission assembly is installed 200 gene sequencer, reduces manufacturing cost, quick and precisely obtains the temperature in gene sequencing box 210, thus realize it is rapid, Accurate cooling and warming and rapid transition temperature, make the result for finally obtaining gene sequencing more accurate.

Preferred embodiment of the present utility model is these are only, the utility model is not limited to, for this area Technical staff for, the utility model can have various modifications and variations.It is all it is of the present utility model spirit and principle within, Any modification, equivalent substitution and improvements made etc., should be included within protection domain of the present utility model.

Claims (10)

1. a kind of liquid transmission assembly, is adapted to be mounted to gene sequencer, and the gene sequencer includes control system, power supply mould Block and liquid transmission assembly, the control system are electrically connected with the power module, the liquid transmission assembly, its feature It is that the liquid transmission assembly includes being used in liquid transfer gun head, gene sequencing box, sewer pipe and drawdown pump, the liquid transfer gun head To accommodate gene sequencing reagent, the liquid transfer gun head is connected with the gene sequencing box, and the gene sequencing box is by described useless Liquid pipe is connected with the drawdown pump, and the gene sequencing box includes housing and dividing plate, and the housing is provided with accommodating chamber, it is described every Plate is arranged at the housing and interlayer is formed between the housing, and genetic chip, the interlayer are provided with the accommodating chamber Temperature sensor and Peltier are inside provided with, the temperature sensor is electrically connected with the control system respectively with the Peltier Connect.

2. transmission assembly according to claim 1, it is characterised in that the dividing plate includes the first dividing plate, described first every Plate is arranged at the bottom of the gene sequencing box, and the temperature sensor is arranged at the housing.

3. transmission assembly according to claim 2, it is characterised in that the housing is provided with away from the side of the accommodating chamber Fin, the thickness of the housing in the region that the fin is surrounded is less than the institute outside the region surrounded positioned at the fin The thickness of housing is stated, the temperature sensor interference is embedded at the fin.

4. transmission assembly according to claim 2, it is characterised in that the dividing plate includes second partition, described second every Plate forms the second interlayer with least one side wall of the gene sequencing box, and the Peltier is arranged in second interlayer, The position of the second partition correspondence Peltier is provided with louvre.

5. transmission assembly according to claim 4, it is characterised in that the Peltier is arranged at the outer wall of the housing.

6. described transmission assembly according to claim 4, it is characterised in that the thickness of the Peltier is less than the described second folder The thickness of layer.

7. transmission assembly according to claim 1, it is characterised in that the temperature sensor is infrared temperature-test sensor.

8. transmission assembly according to claim 1, it is characterised in that also including surge flask, the surge flask passes through pipeline It is connected between the drawdown pump and the sewer pipe.

9. transmission assembly according to claim 1, it is characterised in that also including controlled valve, the controlled valve is set In the sewer pipe.

10. a kind of gene sequencer, it is characterised in that it is installed and is transmitted just like the liquid described in claim 1-9 any one Component.