CN206192892U - Lactoperoxidase's quantitative determination kit - Google Patents
Lactoperoxidase's quantitative determination kit Download PDFInfo
- Publication number
- CN206192892U CN206192892U CN201621138254.0U CN201621138254U CN206192892U CN 206192892 U CN206192892 U CN 206192892U CN 201621138254 U CN201621138254 U CN 201621138254U CN 206192892 U CN206192892 U CN 206192892U
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- bottle
- lactoperoxidase
- reagent
- reagent bottle
- fixed plate
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Lactoperoxidase's quantitative determination kit, it relates to peroxidase's in a raw materials breast and the dairy products quantitative determination kit to it exists loaded down with trivial details, the consuming time length of detection procedures, the great problem of operate miss to solve the current lactoperoxidase of detection. Fixed plate 5 sets up in box body 6, do first reagent bottle 1, second reagent bottle 2, test paper strip 3 and color measurement instrument 4 set up respectively in first bottle hole 6 1, second bottle hole 6 2, test paper strip recess 6 3 with color measurement instrument recess 6 4 in, first reagent bottle 1 is equipped with lactoperoxidase standard substance and hydrogen peroxide solution respectively with second reagent bottle 2, and 5 usefulness cardboards of fixed plate or foam board are made, and 6 usefulness cardboards of box body or thin plastic slab are made. The utility model is used for the short -term test of peroxidase content in raw materials breast and the goods.
Description
Technical field
The utility model is related to a kind of peroxide enzyme detection kit, and in particular to peroxide in a kind of cow's milk and its product
The quick detection kit of compound enzyme content.
Background technology
Lactoperoxidase is a kind of natural antimicrobial substance being present in animal breast, and the property should in milk and milk productses
With extensive.In diary product process, whether lactoperoxidase is commonly used to one of inspection sufficient index of pasteurize.Mirror
Acted in the important biological function of lactoperoxidase and operation instruction, determine lactoperoxidase content and activity for protecting
Card dairy food quality and extension dairy products shelf life have very important significance.
Research both at home and abroad to lactoperoxidase detection is more.Existing detection method is determined by large-scale instrument
Enzymatic activity and enzyme concentration, the degree of accuracy of method, sensitivity are higher, but due to live inspection need to be not suitable for by large-scale instrument
Survey.At present, in view of the instrument detection method of lactoperoxidase, complicated pre-treatment need to be carried out to sample and process time is long, nothing
The shortcomings of method realizes on-line checking.Therefore, the key technology of quick, sensitive, accurate detection lactoperoxidase content is developed, is used
Checked and accepted and its dairy products processing in raw milk, with most important theories researching value and practical application meaning.
Utility model content
The purpose of this utility model is to solve to exist in the analysis test method of existing lactoperoxidase content
It is cumbersome and need technical professional to be operated or high cost or the problems such as on-line checking cannot be realized, there is provided
A kind of quick, easily lactoperoxidase immue quantitative detection reagent box.
The utility model includes the first reagent bottle 1, the second reagent bottle 2, reagent strip 3, colour photometer 4, fixed plate 5 and box body 6,
The fixed plate 5 is arranged in box body 6, and first bottle of hole 6-1, second bottle of hole 6-2, test strips groove are respectively equipped with fixed plate 5
6-3 and colour photometer groove 6-4, the first reagent bottle 1 is arranged in first bottle of hole 6-1, and the second reagent bottle 2 is arranged on second bottle of hole 6-
In 2, test strips 3 are arranged in test strips groove 6-3, and colour photometer 4 is arranged in colour photometer groove 6-4, are filled in the first reagent bottle 1
There are lactoperoxidase standard items, hydrogenperoxide steam generator is housed in the second reagent bottle 2.
The utility model has the advantages that：Lactoperoxidase is prepared by manufacturer's weighing and hydrogenperoxide steam generator is molten
Liquid, and be sequentially loaded into respectively in the first reagent bottle 1 and the second reagent bottle 2, then the first reagent bottle 1, the second reagent bottle 2 are loaded into box
In body 6, when the activity of peroxidase in needing to raw milk and its product is detected, operator can say according to kit
The reagent that bright book is prepared, and being used according to given operating procedure, it is possible in testing and analyzing raw milk and its product
Peroxidase content.The utility model simplifies many numerous and diverse step in experimental implementation, can save the time of experimenter,
Operating error is small.
Brief description of the drawings
Fig. 1 is overall structure diagram of the present utility model.
Specific embodiment
Specific embodiment one：Present embodiment is illustrated with reference to Fig. 1, present embodiment includes that the first reagent bottle 1, second is tried
Agent bottle 2, reagent strip 3, colour photometer 4, fixed plate 5 and box body 6, the fixed plate 5 are arranged in box body 6, are set respectively in fixed plate 5
There are first bottle of hole 6-1, second bottle of hole 6-2, test strips groove 6-3 and colour photometer groove 6-4, the first reagent bottle 1 is arranged on first
In bottle hole 6-1, the second reagent bottle 2 is arranged in second bottle of hole 6-2, and test strips 3 are arranged in test strips groove 6-3, colour photometer 4
It is arranged in colour photometer groove 6-4, lactoperoxidase standard items is housed in the first reagent bottle 1, was equipped with the second reagent bottle 2
Hydrogen peroxide solution.Fixed plate 5 is made of cardboard or plastic foam plate, and box body 6 is made of cardboard or plastic board.Cardboard,
The characteristics of fixed plate 5 and box body 6 that plastic board or plastic foam plate are made have lightweight, easy to carry.
Specific embodiment two：Present embodiment is illustrated with reference to Fig. 1, the first reagent bottle 1 of present embodiment is brown glass
Glass bottle.Bottle inner sealing is packaged with lactoperoxidase standard items, and this is reagent I, -20 DEG C of preservations.Other compositions and annexation
It is identical with specific embodiment one.
Specific embodiment three：Present embodiment is illustrated with reference to Fig. 1, the second reagent bottle 2 of present embodiment is brown modeling
Material bottle.Bottle inner sealing is packaged with hydrogenperoxide steam generator, and this is reagent II, and normal temperature is preserved.Other composition and annexation with it is specific
Implementation method one is identical.
Specific embodiment four：Present embodiment is illustrated with reference to Fig. 1, the test strips 3 of present embodiment are quantified by middling speed
Filter paper is made, and is sealed using vacuum packaging, 4 DEG C of preservations.Other compositions and annexation are identical with specific embodiment one.
Specific embodiment five：Present embodiment is illustrated with reference to Fig. 1, the colour photometer 4 of present embodiment is photoelectric color measurement instrument.
The photoelectric color measurement instrument is mainly made up of three parts and is respectively：Mechanical system, electro-optical system, circuit system.Normal temperature is preserved.Other groups
Into and annexation it is identical with specific embodiment one.
The utility model application method：
First, the preparation of reagent：
It is prepared by lactoperoxidase standard serial solution：Reagent I is taken out, 10mL deionized waters are used, final concentration point is diluted to
Wei not 0mg/mL, 1mg/mL, 2mg/mL, 5mg/mL, the peroxidase mark of 10mg/mL, 15mg/mL, 20mg/mL, 25mg/mL
Quasi- liquid.Face used time preparation.
2nd, the treatment of testing sample：
1. the treatment of liquid sample：
Take liquid sample and 20min (4000r/min, 1~5 DEG C) is centrifuged in refrigerated centrifuge, then removal precipitation utilizes
Distilled water diluting is mixed to debita spissitudo, standby.
2. the treatment of solid sample：
After various types of solid samples are crushed, 0.05g samples are weighed, using distilled water diluting to debita spissitudo, mixed
It is even, it is standby.
3rd, detecting step：
During detection paper, in the test strips for preparing, 3 μ L hydrogenperoxide steam generators are first added dropwise, then 3 μ L breasts to be detected are added dropwise
Sample, after reaction 3min, in determining reflective value on photoelectric color measurement instrument under wavelength 655nm.According to the reflective value of institute's test paper, borrow
Standard curve is helped to understand the content of lactoperoxidase in sample.
Claims (5)
1. the immue quantitative detection reagent box of lactoperoxidase, it includes the first reagent bottle 1, the second reagent bottle 2, reagent strip 3, colour examining
Instrument 4, fixed plate 5 and box body 6, it is characterised in that：The fixed plate 5 is arranged in box body 6, and first is respectively equipped with fixed plate 5
Bottle hole 6-1, second bottle of hole 6-2, test strips groove 6-3 and colour photometer groove 6-4, the first reagent bottle 1 are arranged on first bottle of hole 6-1
In, the second reagent bottle 2 is arranged in second bottle of hole 6-2, and test strips 3 are arranged in test strips groove 6-3, and colour photometer 4 is arranged on
In colour photometer groove 6-4, lactoperoxidase standard items are housed in the first reagent bottle 1, hydrogen peroxide is housed in the second reagent bottle 2
Solution, fixed plate 5 is made of cardboard or plastic foam plate, and box body 6 is made of cardboard or plastic board.
2. the immue quantitative detection reagent box of lactoperoxidase according to claim 1, it is characterised in that：First reagent bottle
1 is Brown Glass Brown glass bottles and jars only.
3. the immue quantitative detection reagent box of lactoperoxidase according to claim 1, it is characterised in that：Second reagent bottle
2 is brown plastic bottle.
4. the immue quantitative detection reagent box of lactoperoxidase according to claim 1, it is characterised in that：The test strips 3 are
Middling speed quantitative filter paper article, is sealed using vacuum packaging.
5. the immue quantitative detection reagent box of lactoperoxidase according to claim 1, it is characterised in that：The colour photometer 4 is
Photoelectric color measurement instrument.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621138254.0U CN206192892U (en) | 2016-10-20 | 2016-10-20 | Lactoperoxidase's quantitative determination kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621138254.0U CN206192892U (en) | 2016-10-20 | 2016-10-20 | Lactoperoxidase's quantitative determination kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206192892U true CN206192892U (en) | 2017-05-24 |
Family
ID=58732254
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621138254.0U Expired - Fee Related CN206192892U (en) | 2016-10-20 | 2016-10-20 | Lactoperoxidase's quantitative determination kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206192892U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114674816A (en) * | 2022-04-18 | 2022-06-28 | 广州新原生物科技有限公司 | Lactoperoxidase detection card and preparation method and application thereof |
-
2016
- 2016-10-20 CN CN201621138254.0U patent/CN206192892U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114674816A (en) * | 2022-04-18 | 2022-06-28 | 广州新原生物科技有限公司 | Lactoperoxidase detection card and preparation method and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170524 Termination date: 20191020 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |