CN206192842U - Flow cytometer based on microfluid lens - Google Patents
Flow cytometer based on microfluid lens Download PDFInfo
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- CN206192842U CN206192842U CN201620727185.0U CN201620727185U CN206192842U CN 206192842 U CN206192842 U CN 206192842U CN 201620727185 U CN201620727185 U CN 201620727185U CN 206192842 U CN206192842 U CN 206192842U
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- China
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- microcavity
- sandwich layer
- runner
- covering
- flow cytometer
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Abstract
The utility model provides a flow cytometer based on microfluid lens, includes cell passageway and waveguide, flow cytometer still includes the microfluid lens, arrange between cell passageway and the waveguide the microfluid lens, the fluid lenticule includes microcavity, sandwich layer runner, covering runner and export runner, the covering runner with the round on every side of the import of microcavity all communicates, sandwich layer runner and sandwich layer entry intercommunication, the internal diameter of sandwich layer entry compares the internal diameter of microcavity is little, just sandwich layer entry with the microcavity is on the same axis line, the exit of sandwich layer entry with covering runner intercommunication and just right the import of microcavity, the export of microcavity with export runner intercommunication. The utility model provides a dynamic verification ability is strong, the good flow cytometer based on microfluid lens of suitability.
Description
Technical field
The utility model is related to integrated-type optics, belongs to a kind of flow cytometer, especially using focal length and focal spot
The flow cytometer of the adjustable microfluid lens of dynamic.
Background technology
Flow cytometer processes and mass produces potential cost control due to its portability, small size, convenience
Deng a little, there is huge development potentiality in biomedical sector.Optical fiber or waveguide are used mostly on current flow cytometer
Light beam is focused into detection zone.But this way only has less numerical aperture, the accuracy of detection is influenceed, and it is this
Design have impact on the integrated of " chip lab " to a certain extent.J.Godin et al. is integrated by a microfluid lens
(the Jessica Godin in flow cytometer；Victor Lien；and Yu-Hwa Lo,Demonstration of two-
dimensional fluidic lens for integration into microfluidic flow cytometers,
APPLIED PHYSICS LETTERS 89,061106(2006)；That is Jessica Godin；Victor Lien；And Yu-Hwa
Lo, for the two dimensional fluid lens example of integrated microfluidic flow cytometer, Applied Physics journal 89,061106 (2006)), carry
Detecting system on a kind of simple, tight, piece that consuming is few is supplied.But the system and most of system can only provide one admittedly
The focal spot of sizing, when to variety classes (size) cell detection, accuracy is not high.
The content of the invention
In order to the dynamic detection ability for overcoming the shortcomings of existing flow cytometer is weaker, applicability is poor, the utility model
There is provided a kind of dynamic detection ability the flow cytometer based on microfluid lens good compared with strong, applicability.
The utility model solves the technical scheme that its technical problem used：
A kind of flow cytometer based on microfluid lens, including cell passage and waveguide, the flow cytometer are also wrapped
Microfluid lens are included, the microfluid lens are arranged between the cell passage and waveguide；The fluid lenticule include microcavity,
Sandwich layer runner, covering runner and outlet flow, the covering runner make a circle with the week of the import of the microcavity and connect, described
Sandwich layer runner is connected with sandwich layer entrance, and the internal diameter of the sandwich layer entrance is smaller than the internal diameter of the microcavity, and the sandwich layer entrance with
On same axis line, the exit of the sandwich layer entrance is with the covering flow passage and just to the microcavity for the microcavity
Import, the outlet of the microcavity is connected with the outlet flow.
Further, the covering runner contain 2 runners, sandwich layer is wrapped up by fillet design, and the covering runner with
The axis of the microcavity is in be mutually perpendicular to arrangement.
Further, the distance between the fluid lenticule and cell passage are 150~250 μm, in the range of this, focal spot
And focal length has good dynamic regulation ability.
Technology design of the present utility model is：Graded index fluid lenticule (L-GRIN) is based on different refractivity laminar flow
Diffusion and convective principles work, rather than rely on fixed liquid-liquid curved surface, therefore do not need laminar velocity high, and micro-
Lens are, by dynamic regulation fluid condition, rather than to change micro-lens surface curvature and realize gradually changed refractive index, therefore its optics
Characteristic can be with real-time regulation.From principle, the utility model can be by 2 covering runner flow velocitys and liquid quality fraction
Regulation, so as to the hot spot and focal length to light beam realize dynamic regulation in 2 dimensions.
The beneficial effects of the utility model are mainly manifested in：Strengthen the integrated level of flow cytometer, it is dynamic according to detection demand
State adjusting focal length, focal spot, to reach more accurate detection purpose.
Brief description of the drawings
Fig. 1 is the overall structure diagram that microfluid lens are used for the utility model flow cytometer optical detection system.
Fig. 2 is the L-GRIN structural representations in Fig. 1.
Fig. 3 is the utility model cavity schematic diagram, and equivalent sandwich layer entrance and covering entrance are identified in figure.
Fig. 4 is refractive index profile in chamber under specific flow velocity.
Fig. 5 is the focused view of index distribution under Fig. 4.
Under the conditions of Fig. 6 is identical flow velocity, the focused view under different solutions mass fraction.
Under Fig. 7 is same solution mass fraction, situation focal spot size figure different in flow rate.
Fig. 8 be it is different in flow rate under when different focal spot sizes (i.e.), focused at 200 μm corresponding to liquid quality fraction
Figure.
Specific embodiment
The utility model is further described below in conjunction with the accompanying drawings.
Referring to Figures 1 and 2, a kind of flow cytometer based on microfluid lens, including cell passage 6 and waveguide 7, it is described
Flow cytometer also includes microfluid lens 8, and the microfluid lens 8 are arranged between the cell passage 6 and waveguide 7；It is described
Fluid lenticule 8 includes microcavity, sandwich layer runner, covering runner and outlet flow, the import of the covering runner and the microcavity
Make a circle in week and connect, the sandwich layer runner is connected with sandwich layer entrance, and the internal diameter of the sandwich layer entrance is than in the microcavity
Footpath is small, and the sandwich layer entrance with the microcavity on same axis line, the exit of the sandwich layer entrance and the covering stream
Road is connected and just to the import of the microcavity, and the outlet of the microcavity is connected with the outlet flow.
Further, the covering runner contain 2 runners, sandwich layer is wrapped up by fillet design, and the covering runner with
The axis of the microcavity is in be mutually perpendicular to arrangement.
As shown in Fig. 2 wherein 2,3 passages are covering liquid inlet to microfluid lens arrangement, and 1 passage enters for sandwich layer liquid
Mouthful, 4,5 passages are exported for fluid-mixing.Integrated approach is as shown in figure 1, fluid lenticule is fixed at 200 μm of cell passage；
Certainly, the distance between the fluid lenticule and cell passage can also select any other point in 150~250 μ ms
Value.
The sandwich layer runner and two covering runner parallel arrangements.In the present embodiment, sandwich layer liquid is from sandwich layer liquid inlet 1
Injection, covering liquid injects from covering liquid inlet 2,3, in order to exclude influence of the flow velocity direction to liquid laminar flow, covering entrance
Fillet design is used, changes velocity vector direction, mixing liquid exports 4,5 and flows out by fluid-mixing respectively.Fluid is micro-
The major part of mirror is middle mixing chamber, and sandwich layer liquid occurs diffusion convection effect wherein with covering liquid, forms gradual change
Index distribution.The equivalent structure of fluid lenticule cavity as shown in figure 3, the entrance length of side of passage 1 is 50 μm, passage 2,3,4,
5 are 50 μm of * 150 μm of rectangles.
Ethylene glycol solution (sandwich layer liquid) is with deionized water (DI, covering liquid) while injecting cavity, xoy and yoz cuts
Axisymmetric graded--index planar waveguides on face：Paraxial refractive index is maximum, along chamber central axial direction and vertical axis direction
Index distribution is gradually reduced.
Simulated using FInite Element (FEM) and ray tracing method and Optimal Parameters.The index distribution of device can lead to
Concentration after crossing simulation and calculating two-phase fluid convection current and diffusion process stably in cavity is obtained.In cavity, the expansion of fluid
Dissipate the index distribution that fluid lenticule is have impact on convection current, during Convention diffusion, U=(Qcore+Qclad)/R2Represent cavity
Fluid velocity, QcoreAnd QcladThe flow velocity of sandwich layer and covering is represented respectively, and R is the length of side.
The larger analog result of diffusion coefficient is along the diffusion effect of laterally longitudinal direction it is obvious that opposite diffusion coefficient is less
Index distribution fade effect is not obvious.This explanation, diffusion coefficient is a very effective control graded--index planar waveguides
Means, so also directly affects the performance of lenticule.Due to the change of environment temperature, liquid diffusion coefficient will be produced very big
Influence, therefore the regulation of diffusion coefficient can realize by changing fluid temperature.Assuming that deionized water and ethylene glycol are divided
Do not elect covering and sandwich layer liquid as, and assume that covering sandwich layer flow rate of liquid is identical and is calculated without Relative sliding.Low flow velocity
Under can realize effective focus adjustment, the regulation of focal spot size can be realized under high flow rate.
By adjusting the flow velocity of each entrance, the distributed effect of different refractivity is obtained, so as to adjust focal spot and focal length.
The influence of liquid quality fraction：Because the concentration of solution will directly affect diffusion coefficient, the change of diffusion coefficient will
Directly influence the change of lenticule.In spreading convection process in microcavity, the effect spread along flow direction is more and more brighter
It is aobvious.However, this diffusion causes the strength of fluid in diverse location different, the change of this concentration is by direct reaction in each position
On the diffusion coefficient put, therefore diffusion coefficient is not invariable in whole process.This phenomenon is outstanding in concentrated solution
It is protrusion.So far, the definite expression formula of the Changing Pattern of the phenomenon also neither one can be described.Therefore, for simplification
The complexity of calculating, is not solution very high using concentration, using the ethylene glycol solution of mass fraction 0.2, in diffusion convection current
During emulated using constant diffusion coefficient D.
Because the utility model contains 4 covering entrances, it is possible to achieve the refractive index dynamic regulation effect on two-dimensional surface, can be with
It is applied to more different testing requirements.
Fig. 4 is to be 25*10 in four covering passages and sandwich layer channel velocity3The equivalent folding obtained in the case of pL/s
It is then the focusing schematic diagram obtained by ray tracing method to penetrate rate distribution and Fig. 5.
It is 40*10 that Fig. 6 is given in covering flow velocity3PL/s, index distribution occurs during adjustment quality of glycol fraction
Change.When sandwich layer liquid quality fraction is increased, corresponding focal length reduces therewith, and 6 rank fitting functions are：F (b)=9.03*
106*b6-1.30*107*b5+7.65*106*b4-2.36*106*b3+416377.0*b2-42645.0*b+2342.6.Wherein b
It is liquid quality fraction, F (b) is focal length.By this property, we can adjust fluid lenticule focal length, make it different in flow rate
Under, remain to focus on detection zone.
Fig. 7 gives the graph of a relation of covering flow velocity and focal spot size, by the emulation under different covering flow velocitys, Wo Menke
To obtain one 5~50 μm of tunable focal spot, cell size is generally 10~50 μm, can meet the accurate inspection of most cells
Survey.Because covering flow velocity can also influence light beam focal length, adjust focal spot size after, we should by adjusting liquid quality fraction,
Keep focal length at 200 μm, focus on monitored area.Fig. 8 is given under different covering flow velocitys, is focused on corresponding to 200 μm
Liquid quality fraction.This flexibly adjustable light beam regulating effect can be detected in biomedicine, especially on flow cytometer
There is important application.
Claims (3)
1. a kind of flow cytometer based on microfluid lens, including cell passage and waveguide, it is characterised in that：The streaming is thin
Born of the same parents' instrument also includes microfluid lens, and the microfluid lens are arranged between the cell passage and waveguide；The fluid lenticule
Including microcavity, sandwich layer runner, covering runner and outlet flow, the import of the covering runner and the microcavity makes a circle in week
Connection, the sandwich layer runner is connected with sandwich layer entrance, and the internal diameter of the sandwich layer entrance is smaller than the internal diameter of the microcavity, and the core
With the microcavity on same axis line, the exit of the sandwich layer entrance is with the covering flow passage and just to institute for layer entrance
The import of microcavity is stated, the outlet of the microcavity is connected with the outlet flow.
2. the flow cytometer of microfluid lens is based on as claimed in claim 1, it is characterised in that：The covering runner contains 2
Individual runner, is wrapped up sandwich layer by fillet design, and the covering runner and the axis of the microcavity are in be mutually perpendicular to arrangement.
3. the flow cytometer of microfluid lens is based on as claimed in claim 1 or 2, it is characterised in that：The fluid is micro-
The distance between mirror and cell passage are 150 μm~250 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620727185.0U CN206192842U (en) | 2016-07-06 | 2016-07-06 | Flow cytometer based on microfluid lens |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620727185.0U CN206192842U (en) | 2016-07-06 | 2016-07-06 | Flow cytometer based on microfluid lens |
Publications (1)
| Publication Number | Publication Date |
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| CN206192842U true CN206192842U (en) | 2017-05-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620727185.0U Expired - Fee Related CN206192842U (en) | 2016-07-06 | 2016-07-06 | Flow cytometer based on microfluid lens |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206192842U (en) |
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2016
- 2016-07-06 CN CN201620727185.0U patent/CN206192842U/en not_active Expired - Fee Related
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170524 Termination date: 20210706 |