CN206188794U - Photo -bioreactor - Google Patents

Photo -bioreactor Download PDF

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Publication number
CN206188794U
CN206188794U CN201621173332.0U CN201621173332U CN206188794U CN 206188794 U CN206188794 U CN 206188794U CN 201621173332 U CN201621173332 U CN 201621173332U CN 206188794 U CN206188794 U CN 206188794U
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China
Prior art keywords
reactor body
diversion column
bioreactor
reactor
nostoc
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CN201621173332.0U
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Inventor
熊斌
安言君
董美丽
王远忠
陈牧
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HUNAN YANDI BIOLOGICAL ENGINEERING Co Ltd
CHANGDE YANDI BIOLOGICAL TECHNOLOGY Co Ltd
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HUNAN YANDI BIOLOGICAL ENGINEERING Co Ltd
CHANGDE YANDI BIOLOGICAL TECHNOLOGY Co Ltd
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Abstract

The utility model relates to a biont cultivation equipment field discloses a photo -bioreactor. It includes the reactor main part, and it is still including setting up the transparent water conservancy diversion post in the reactor main part, the height that highly is less than the reactor main part of water conservancy diversion post, the department is provided with the entry in the lateral wall bottom of water conservancy diversion post, and the portion is provided with aeration equipment at the water conservancy diversion toe. The utility model provides a photo -bioreactor can avoid ventilating afterwards at the reactor effectively, and the nostoc forms the sinuous flow and causes the part to spin and hang the limit phenomenon at reactor to the smooth reaction time of nostoc can be effectively prolonged, and then the volume of reactor is enlarged.

Description

A kind of bioreactor
Technical field
The present invention relates to biological culture equipment field, more particularly to a kind of bioreactor.
Background technology
Existing frequently-used nostoc cultivation reactor is airlift photobioreactor, because nostoc is photoautotrophy algae Class, the CO needed for mainly using the means of ventilation to make nostoc in suspended state and light reaction being provided2, then produce reaction with light Carry out photoautotrophy.Bioreactor mainly includes cylindrical and square cylinder formula reactor.In the cultivation of nostoc, light is passed through Depth is a conclusive index, usually no more than 40cm.Calculated i.e. since the contact surface of light is seen, light transit dose exists Below 100lx is just decayed to after 40cm.Due to nostoc color depth, to blocking it is obvious that so typically cultivating nostoc for light The volume of drum or square cylinder is not too large, generally between 100-300 liters, and drives current using aeration stone outlet Flowing makes nostoc be moved along the flowing of current in container.
However, for cylindrical reactor, the suspended state if necessary to keep nostoc, it is necessary to provide enough pressure Air, particularly nostoc as macro diameter more than 2mm after, air pressure deficiency is easy for causing nostoc The phenomenon with hanging end side is sunk to the bottom, so throughput is very big, the flowing of whole water body has thus been driven and because of the mutual of current Act on and cause to have local whirlpool, the phenomenon that nostoc spins in these whirlpools in part, if whirlpool is in black light Position can then cause nostoc to be constantly in lose light state and can not complete photosynthesis.And because throughput is big, current Speed is fast, and light dark reaction exchange frequency is fast, and the growth to nostoc is unfavorable.Because these limit the body for causing cylindrical reactor Product is little, typically below 200 liters.
Although and square cylinder formula reactor can amplify in volume, due to the shape issue of square container so that logical During air culture is supported, nostoc presence sinks to the bottom and is interacted with current, can produce substantial amounts of sinking to the bottom in intersection, it is impossible in suspension State, thus influence the growth of nostoc.
The content of the invention
(1) technical problem to be solved
It is an object of the invention to provide a kind of bioreactor, to avoid after reactor is ventilated nostoc in reactor It is interior to form sinuous flow and cause locally to spin and hang side phenomenon, and can effectively extend the optical response time of nostoc, and then expand The volume of reactor.
(2) technical scheme
In order to solve the above-mentioned technical problem, the invention provides a kind of bioreactor, including reactor body, its In, the bottom surface of the reactor body is indent, also including the transparent diversion column being arranged in reactor body, the water conservancy diversion The height of post is less than the height of reactor body, and at the most deep position in the bottom surface of reactor body, in the side of diversion column Wall is provided with entrance at bottom, and water conservancy diversion column bottom is provided with aerator.
Wherein, the reactor body is cylindrical shape, and the diversion column is cylindrical shape, and the reactor body and described Diversion column is coaxial.
Wherein, the bottom surface of the reactor body uses spherical, and the centre of sphere of the spherical is located at the axle of the diversion column On line.
Wherein, the bottom of the reactor body is provided with the guide groove being adapted to the side wall of the diversion column, the water conservancy diversion Column bottom is pasted onto in the guide groove of the reactor body bottom.
Wherein, the bottom of the diversion column is provided with support feet, the bottom of the support feet is pasted onto the reactor master In the guide groove of body bottom.
Wherein, the entrance is multiple, and multiple entrances are evenly distributed on the side wall of diversion column.
Wherein, the reactor body and the diversion column are made by lucite.
Wherein, being smaller than between reactor body side wall and diversion column side wall is equal to 40cm.
Wherein, the reactor body bottom is provided with osculum, the osculum is centrally located at the diversion column On axis.
(3) beneficial effect
Bioreactor involved in the present invention guides nostoc by setting guide shell in reactor body Motion, prevents influence of the sinuous flow to nostoc circulatory motion, and each nostoc individuality can be entered along round and round track Row operation, and the slow effect in shape of holding court from behind a screen can be formed after frond reaches the water surface of container, so that see that light is uniform, Avoid some individuals due to the influence of sinuous flow, occur seeing that light is uneven, available energy in the place motion of bottom or insufficient light The low problem of consumption;Quickening frond is additionally, since in the speed of service at unglazed place, is slowed down the speed of service at visible ray and is made Pueraria lobota Celestial rice can run the longer time optical position is seen, the need for meeting photosynthetic response, thus can increase the body of reaction vessel Product, makes its diameter increase on the basis of existing and is twice the above, large-scale column reactor is can be used in the culture of nostoc, So as to increase the yield per unit area, reduce the biological proportion of goods damageds and the death rate.Because using a small amount of water body, quickly flowing is whole to drive The flowing of individual water body, then the water body flow of bottom is slow, and the present invention makes nostoc frond by using reactor body bottom concave surface It is gathered near the guide shell of middle part so that only can just drive substantial amounts of water body to form a circulation with a small amount of throughput, because And greatly save throughput, that is, the energy is saved, contrast existing frequently-used culture vessel, energy consumption reduction half.Institute of the present invention The bioreactor being related to can be adapted to the culture of various algae, including colony state algae and unicellular alga.
Brief description of the drawings
Fig. 1 is a sectional view for preferred embodiment according to bioreactor of the invention.
In figure, 1:Reactor body;2:Diversion column;3:Osculum;4:Guide groove;5:Entrance.
Specific embodiment
With reference to the accompanying drawings and examples, specific embodiment of the invention is described in further detail.Following instance For illustrating the present invention, but it is not limited to the scope of the present invention.
Fig. 1 shows a preferred embodiment of bioreactor of the invention.As shown in figure 1, the photo-biological Reactor includes reactor body and the transparent diversion column 2 being arranged in reactor body, wherein, the height of diversion column 2 is less than The height of reactor body, is provided with entrance 5 in the bottom sidewall of diversion column 2, it is preferable that the quantity of entrance 5 is multiple, this Multiple entrances 5 are evenly distributed on the sidewall bottom of diversion column 2.Additionally, the bottom of diversion column 2 is provided with aerator (not showing Go out).Nostoc enters along the entrance 5 of the bottom of diversion column 2, and in the presence of aerator, nostoc runs to diversion column 2 and pushes up End starts uniformly to be dispersed on the water surface, and is run to surrounding, is settled until reactor body bottom with the speed for slowing down.By The direction of current is limited in middle diversion column 2 so that the kinetic energy that all zones of aeration come runs up all along diversion column 2, band Dynamic frond is moved together, in the exit of diversion column 2, the water column being driven run up and it is spherical in shape diffuse into, in fact with few The current of amount promote the current of the outer whole container of diversion column 2, so speed can slow down, form the running orbit of cascade shape.
Additionally, the bottom 4 of reactor body uses the spherical of indent, the centre of sphere of the spherical to be located at the axle of diversion column 2 On line.The flowing of whole water body is driven due to quickly being flowed using a small amount of water body, then the water body flow of bottom is slow, it is necessary to borrow Bottom concave surface makes nostoc frond be gathered near the diversion column 2 of middle part, and such spherical bottom could be used plus middle diversion column 2 A small amount of throughput forms a circulation driving substantial amounts of water body.Throughput is greatlyd save, that is, saves the energy, contrast is existing In conventional culture vessel, energy consumption reduction half.
It should be noted that, although in this embodiment, the bottom 4 of reactor body uses the spherical of indent.Ability Field technique personnel should be appreciated that the bottom 4 of the reactor body also can be using the taper surface or other shapes of inner concave of indent.
Specifically, in this embodiment, reactor body is cylindrical shape, and diversion column 2 is also cylindrical shape, and reactor body It is coaxial with diversion column 2.It should be noted that it will be appreciated by those skilled in the art that the reactor body and diversion column 2 Shape can also be other shapes, such as square.
The bottom 4 of reactor body is provided with the guide groove being adapted to the side wall of diversion column 2, and the bottom of diversion column 2 is pasted onto In the guide groove of reactor body bottom 4.
Further, current are waited to bring middle diversion column 2 into order to prevent frond from needing, the lower end of diversion column 2 is provided with Three supporting legs, the bottom of the support feet is pasted onto in the guide groove of reactor body bottom 4, to expand entering for the bottom of diversion column 2 Mouth region domain, it is to avoid frond is blocked in the region between two entrances 5.
Further, since nostoc self color is partially blackish green, the density for adding cultivation is very big, and sunlight exists into culture vessel Decay in water can be severe, so the penetratingdepth of light is 40cm or so in general cultivation, i.e., is opened from the contact surface for seeing light Begin to calculate, light transit dose just decays to below 100lx after 40cm.Therefore between reactor body side wall 1 and the side wall of diversion column 2 Be smaller than be equal to 40cm.
Preferably, reactor body bottom 4 is provided with osculum 3, the osculum 3 is centrally located at the axle of diversion column 2 On line.
Reactor body and diversion column 2 are made by lucite.
The preparation method of above-mentioned bioreactor, it is comprised the following steps:The side of reactor body is integrally formed first Wall 1;The bottom 4 of reactor body is polished into the sphere (such as sphere most recess is 5mm) of indent, and in disc heartcut Guide groove simultaneously opens up osculum;Then the side wall 1 of reactor body and bottom 4 are bonded together by binders such as AB glue;Treat After thoroughly gluing, diversion column 2 is first cut into gateway, the bottom of diversion column 2 is pasted onto by guide groove by binders such as 502 glue In, and filled and led up remaining guide groove with binders such as AB glue.Last just aerator is arranged on the bottom of diversion column 2.
The bioreactor guides the motion of nostoc by the diversion column 2 at middle part, prevents sinuous flow to nostoc ring The influence of shape motion, each nostoc individuality can be run along round and round track, and reaches anti-in frond The slow effect in shape of holding court from behind a screen can be formed after answering the water surface of device, so that seeing that light is uniform, it is to avoid some individuals are due to sinuous flow Influence, in the place motion of bottom or insufficient light, and see that light is uneven, the low problem of available energy dissipation.Further, since accelerating Frond slows down the speed of service at visible ray and frond is being seen that optical position operation is longer in the speed of service at unglazed place Time, the need for meeting photosynthetic response.Therefore the volume of reaction vessel can be increased, its diameter is increased on the basis of existing The above is twice, large-scale column reactor is can be used in the culture of nostoc, so as to increase the yield per unit area, reduce life The thing proportion of goods damageds and the death rate.Present invention condition of photosynthetic response according to needed for nostoc growth is designed, that is, reach nostoc The time of intensity needed for photosynthetic response is at 8 more than hour.Nostoc is allowed daytime to be transported in the region of light reaction in the culture vessel It is dynamic to have reached more than 8 hours, thus need not carry out photosynthesis by artificial supplementation light source again.
Presently preferred embodiments of the present invention is the foregoing is only, is not intended to limit the invention, it is all in essence of the invention Within god and principle, any modification, equivalent substitution and improvements made etc. should be included within the scope of the present invention.

Claims (9)

1. a kind of bioreactor, including reactor body, it is characterised in that the bottom surface of the reactor body is indent , also including the transparent diversion column being arranged in reactor body, the height of the height less than reactor body of the diversion column, And at the most deep position in the bottom surface of reactor body, entrance is provided with the sidewall bottom of diversion column, at diversion column bottom Portion is provided with aerator.
2. bioreactor as claimed in claim 1, it is characterised in that the reactor body is cylindrical shape, described to lead Fluidization tower is cylindrical shape, and the reactor body and the diversion column are coaxial.
3. bioreactor as claimed in claim 1, it is characterised in that the bottom surface of the reactor body is using spherical Face, the centre of sphere of the spherical is located on the axis of the diversion column.
4. bioreactor as claimed in claim 1, it is characterised in that the bottom of the reactor body is provided with and institute The guide groove of the side wall adaptation of diversion column is stated, the water conservancy diversion column bottom is pasted onto in the guide groove of the reactor body bottom.
5. bioreactor as claimed in claim 4, it is characterised in that the bottom of the diversion column is provided with support Pin, the bottom of the support feet is pasted onto in the guide groove of the reactor body bottom.
6. bioreactor as claimed in claim 1, it is characterised in that the entrance is multiple, multiple entrances uniformly divide Cloth is on the side wall of diversion column.
7. the bioreactor as any one of claim 1-6, it is characterised in that the reactor body and described Diversion column is made by lucite.
8. the bioreactor as any one of claim 1-6, it is characterised in that reactor body side wall with Being smaller than between the wall of the diversion column side is equal to 40cm.
9. the bioreactor as any one of claim 1-6, it is characterised in that in the reactor body bottom Osculum is provided with, the osculum is centrally located on the axis of the diversion column.
CN201621173332.0U 2016-11-02 2016-11-02 Photo -bioreactor Active CN206188794U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106544263A (en) * 2016-11-02 2017-03-29 常德炎帝生物科技有限公司 A kind of bioreactor and preparation method thereof
CN113796551A (en) * 2020-06-15 2021-12-17 刘世滨 Coffee bean baking cup and baking machine

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106544263A (en) * 2016-11-02 2017-03-29 常德炎帝生物科技有限公司 A kind of bioreactor and preparation method thereof
CN113796551A (en) * 2020-06-15 2021-12-17 刘世滨 Coffee bean baking cup and baking machine

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