CN206115050U - Structured light generates device based on polarized light phase modulation - Google Patents
Structured light generates device based on polarized light phase modulation Download PDFInfo
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- CN206115050U CN206115050U CN201621062724.XU CN201621062724U CN206115050U CN 206115050 U CN206115050 U CN 206115050U CN 201621062724 U CN201621062724 U CN 201621062724U CN 206115050 U CN206115050 U CN 206115050U
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Abstract
The utility model discloses a structured light generates device based on polarized light phase modulation. Optical fiber penetrate that the laser instrument sent linearly polarized light is to the half -wave plate, through half -wave plate the polarization direction rotation angle, resolve into two polarized components again to the electric optical phase modulator in, produce the phase difference by the modulation of electric optical phase modulator, it incides a left side after restrainting to expand through the beam expanding lens after that, let the light beam after modulating see through in the middle of the terminal surface that right polarization spectroscope concatenation back formed, then two way optical fiber penetrate only see through along the weight formation light beam of the same kind of its optical axis direction to inclined to one side vibration -damping sheet, the focus department that finally focuses the focusing mirror takes place to interfere. The utility model discloses on using the micro - formation of image of fluorescence, can obtain better the fluorescence signal SNR and the stronger inside big degree of depth formation of image ability of tissue.
Description
Technical field
This utility model is related to laser application technique field, and in particular to a kind of structure light based on polarization light phase modulation
Generating means.
Background technology
Structured Illumination is to apply a kind of common laser lighting method in micro-imaging field, and Structured Illumination is micro-
Method (SIM) is that Mats doctors Gustafsson of U.S. Janelia Farm mainly research and develop, and this technology is using specific knot
Structure light goes to irradiate sample to be observed.The generation of wherein structure light is by one spatial light modulator (light of insertion in illumination path
Grid or digital micromirror array), illumination light is projected on testing sample by Jing object lens after Grating Modulation, receive in the focal plane of sample
It is also unaffected in focal plane to the irradiation of modulation light, modulate the fluorescence information produced by light and connect by CCD by imaging system
Receive.In Structured Illumination micro-imaging, the structure light in out of phase and direction illuminates successively sample, these structure lights and sample
Moire fringe produced by this different angle mixing is gathered successively and decoded by CCD, that is, extracts high fdrequency components, eventually through
Reconstruction forms the SIM images of ultrahigh resolution.
Current structure light micro imaging method can only carry out micro-imaging to sample surfaces, it is impossible to enough to thick biological tissue
Effectively micro-imaging is carried out at certain depth.Structured Illumination based on polarization light phase modulation of the present utility model is applied
Micro-imaging field can effectively solving this problem, greatly improve the signal to noise ratio of the big depth micro-imaging of biological tissue.
Utility model content
In order to solve problem present in background technology, this utility model provides a kind of based on polarization light phase modulation
Structure light generating means.
The technical solution adopted in the utility model is：
This utility model includes that laser instrument sends light beam successively with laser instrument front edge is arranged in along the transmission locus of light
The half-wave plate of arrangement, electro-optic phase modulator, beam expanding lens, two polarization spectroscopes (PBS) spliced side by side in the horizontal direction, partially
Shake piece and focus lamp；Laser instrument sends the light beam of line polarized light and incides on half-wave plate, through the half-wave plate polarization direction anglec of rotation
Degree, is again incident in electro-optic phase modulator (EOM) being broken down into two polarized components of horizontal direction and vertical direction, two
Polarized component produces phase contrast by electro-optic phase modulator modulation, and then together Jing expands the two-way light beam comprising respective polarized component
Shu Jing is incided after expanding and allow in the middle of the end face of formation after left polarization spectroscope and the splicing of right polarization spectroscope the light beam after modulating
Through then two-way light beam incides the component only transmitted on polaroid along its optical axis direction and forms light beam all the way, final to focus on
Interfere in the focal point of focus lamp.
The direction of vibration of the line polarized light that described laser instrument sends is horizontal direction.
The light beam that described half-wave plate sends perpendicular to laser instrument, optical axis and the horizontal direction of half-wave plate are in 22.5 ° of angles.
The optical axis of described electro-optic phase modulator vertically, and the light beam sent perpendicular to laser instrument.
Two described polarization spectroscopes (PBS) are respectively left polarization spectroscope and right polarization spectroscope, left polarization spectro
Mirror and right polarization spectroscope are close to side by side, and light beam is incided in the middle of left polarization spectroscope and right polarization spectroscope, left polarization spectro
Light beam is divided into the big semicircles such as two by the intermediate line between mirror and right polarization spectroscope, and left avertence is shaken spectroscopical light splitting surface and water
Plane is at 45 ° and be in 45° angle with beam direction, and right avertence shakes spectroscopical light splitting surface and be in horizontal plane and with beam direction
45° angle.
The light beam that described polaroid sends perpendicular to laser instrument, light transmission shaft and the horizontal direction of polaroid are in 45 ° of angles.
Structure light of the present utility model is modulated by electro-optic phase modulator to polarized light, and by two according to special
The polarization spectroscope of orientation splicing carries out light splitting spatially, and line focus mirror forms the interferogram of two-beam after focusing in focal area
Sample, and interference pattern changes over, and causes the cyclically-varying of focal area light distribution, and the light intensity bag in focal volume
DC component and AC compounent (i.e. high fdrequency components) are contained.
This utility model beneficial effect is：Solve the structure light at thick sample tissue certain depth and efficiently generate ground technology
Blank, applies it on fluorescent microscopic imaging, can obtain more preferable fluorescence signal signal to noise ratio and the big depth of higher organization internal
Degree imaging capability.
Description of the drawings
Fig. 1 is the structure chart of this utility model device；
Fig. 2 is this utility model to polarizing the schematic diagram of light phase modulation and light splitting；
Fig. 3 is the position that the splicing orientation and laser beam of two PBS is impinged upon on PBS；
Fig. 4 is P light and the reflection of S light and transmission situation in two PBS；
Fig. 5 is focal area light distribution, different with modulation light and the change of unmodulated light phase contrast.
In figure：Laser instrument 1, half-wave plate 2, electro-optic phase modulator 3, beam expanding lens 4, left polarization spectroscope 5, right polarization spectro
Mirror 6, polaroid 7, focus lamp 8.
Specific embodiment
This utility model embodiment is described in detail below in conjunction with the accompanying drawings.
This utility model system includes that laser instrument 1 sends the half-wave that light beam is sequentially arranged with the front of laser instrument 1 edge is arranged in
Piece 2, electro-optic phase modulator 3, beam expanding lens 4, two polarization spectroscopes (PBS), the and of polaroid 7 that splice side by side in the horizontal direction
Focus lamp 8；
Laser instrument 1 sends the light beam of line polarized light and incides on half-wave plate 2, through the polarization direction anglec of rotation of half-wave plate 2,
It is again incident in electro-optic phase modulator 3 (EOM) being broken down into two polarized components of horizontal direction and vertical direction, two partially
The component that shakes modulates generation phase contrast by electro-optic phase modulator 3, and then together Jing expands the two-way light beam comprising respective polarized component
Beam mirror 4 is incided after expanding and allow in the middle of the end face of formation after left polarization spectroscope 5 and the splicing of right polarization spectroscope 6 light after modulating
Beam is passed through, and then two-way light beam incides the component only transmitted on polaroid 7 along its optical axis direction and forms light beam all the way, final poly-
The burnt focal point in focus lamp 8 is interfered.
Specific implementation process of the present utility model is as follows：
(1) can be with the helium neon laser of 632.8nm as light source, laser instrument sends the light beam of line polarized light and incides half-wave
On piece, the line polarized light direction of vibration of outgoing is horizontal direction, and rotation adjusts optical axis and the level side that half-wave plate causes half-wave plate
To in 22.5 ° of angles, light beam Jing half-wave plate rear polarizers direction is rotated to be with horizontal direction in 45 ° of angles, and now light beam is P to note；
(2) then, light beam P is incided in electro-optic phase modulator (EOM), the optical axis edge of electro-optic phase modulator (EOM)
Vertical direction and the light beam sent perpendicular to laser instrument 1, light beam is broken down into amplitude ratio in the crystal of electro-optic phase modulator
1:1 horizontal direction and the two of vertical direction polarized components, two polarized components are respectively Ex components and Ey components, such as Fig. 2
It is shown；
(3) according to the principle of electro-optic phase modulator, electro-optic phase modulator input EOM square wave letters are given with signal generator
Number, EOM has the modulating frequency of time response quickly and highest 10MHz.Along the polarization of electro-optic phase modulator optical axis direction
Component (Ey) is phase-modulated and the polarized component (Ex) perpendicular to electro-optic phase modulator optical axis direction is not phase-modulated, and one
Individual polarized component is produced with the Phase delay of time mechanical periodicity with respect to another polarized component, from electro-optic phase modulator output
Light beam be designated as M；
(4) and then, light beam M incides the end face of left polarization spectroscope 5 and right polarization spectroscope 6 Jing after beam expanding lens 4 is expanded
On, light beam M is transmitted respectively in two polarization spectroscopes, and the light splitting surface of left polarization spectroscope 5 is with the horizontal 45 ° and and light beam
Direction is in 45° angle in 45° angle, the light splitting surface and horizontal plane of right polarization spectroscope 6 and with beam direction.
As shown in figure 3, the area that light beam is impinged upon on two polarization spectroscopes is identical, it is respectively semi-circular shape.Therefore, it is left
The polarized light component of the permission vertical direction of polarization spectroscope 5 by and 6 tolerable injury level direction polarized light of right polarization spectroscope
Component passes through, so as to modulate light and unmodulated light from spatially separating, along the polarization point of electro-optic phase modulator optical axis direction
The light beam of amount (Ey) passes through left polarization spectroscope 5, perpendicular to the light of the polarized component (Ey) of electro-optic phase modulator optical axis direction
Beam is designated as S by right polarization spectroscope 6, the two-way light beam for passing through.
(5) last, light beam S is incided on polaroid 7, as shown in figure 4, the light that polaroid 7 sends perpendicular to laser instrument 1
Beam, light transmission shaft and the horizontal direction of polaroid 7 are in 45 ° of angles, and the two-beam that light beam S is included is after respectively only by along polarization
The component of the optical axis direction of piece 7, and component amplitude is equal in magnitude, the component line focus mirror of the two-beam for passing through occurs to do in its focus
Relate to, generating structure light.
When phase contrast is 0 between the two-beam Jing after electro-optic phase modulator modulation, there is constructive interference in focal point,
Light intensity in focus lamp focal volume region is largest light intensity；
When phase contrast is π between the two-beam Jing after electro-optic phase modulator modulation, there is destructive interference in focal point,
Focal point light intensity is minimum, and largest light intensity is in focal volume both sides.
The fluorescent inspired by the structure light of the present invention equally has direct current and exchanges two kinds of components, can effectively suppress
Background noise, obtains the ability of fluorescence signal, to biological tissue's big depth fluorescence microscopy when being imaged to organization internal so as to improve
Imaging field has good application prospect.
Claims (6)
1. a kind of based on the structure light generating means for polarizing light phase modulation, it is characterised in that：Along the transmission locus of light, including
Laser instrument (1) and laser instrument (1) front is arranged in along sending half-wave plate (2), electro-optic phase modulator that light beam is sequentially arranged
(3), beam expanding lens (4), two polarization spectroscopes (5,6), polaroid (7) and the focus lamp (8) that splice side by side in the horizontal direction；Swash
Light device (1) sends the light beam of line polarized light and incides on half-wave plate (2), through half-wave plate (2) the polarization direction anglec of rotation, reenters
It is mapped in electro-optic phase modulator (3) and is broken down into two polarized components of horizontal direction and vertical direction, two polarized components
Phase contrast is produced by electro-optic phase modulator (3) modulation, then the Jing beam expanding lens together of the two-way light beam comprising respective polarized component
(4) incide after expanding and allow in the middle of the end face of formation after left polarization spectroscope (5) and right polarization spectroscope (6) splicing after modulation
Light beam is passed through, and then two-way light beam incides the component only transmitted on polaroid (7) along its optical axis direction and forms light beam all the way, most
The focal point for focusing on focus lamp (8) eventually is interfered.
2. according to claim 1 a kind of based on the structure light generating means for polarizing light phase modulation, it is characterised in that：Institute
The direction of vibration of the line polarized light that the laser instrument (1) stated sends is horizontal direction.
3. according to claim 1 a kind of based on the structure light generating means for polarizing light phase modulation, it is characterised in that：Institute
The light beam that the half-wave plate (2) stated sends perpendicular to laser instrument (1), optical axis and the horizontal direction of half-wave plate (2) are in 22.5 ° of angles.
4. according to claim 1 a kind of based on the structure light generating means for polarizing light phase modulation, it is characterised in that：Institute
The optical axis of the electro-optic phase modulator (3) stated vertically, and the light beam sent perpendicular to laser instrument (1).
5. according to claim 1 a kind of based on the structure light generating means for polarizing light phase modulation, it is characterised in that：Institute
Two polarization spectroscopes (5,6) stated are respectively left polarization spectroscope (5) and right polarization spectroscope (6), left polarization spectroscope (5)
It is close to side by side with right polarization spectroscope (6), light beam is incided in the middle of left polarization spectroscope (5) and right polarization spectroscope (6), left avertence
Shake spectroscope (5) light splitting surface with the horizontal 45 ° and with beam direction in 45° angle, the light splitting surface of right polarization spectroscope (6) with
Horizontal plane and with beam direction be in 45° angle.
6. according to claim 1 a kind of based on the structure light generating means for polarizing light phase modulation, it is characterised in that：Institute
The light beam that the polaroid (7) stated sends perpendicular to laser instrument (1), light transmission shaft and the horizontal direction of polaroid (7) are in 45 ° of angles.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621062724.XU CN206115050U (en) | 2016-09-19 | 2016-09-19 | Structured light generates device based on polarized light phase modulation |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201621062724.XU CN206115050U (en) | 2016-09-19 | 2016-09-19 | Structured light generates device based on polarized light phase modulation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106291962A (en) * | 2016-09-19 | 2017-01-04 | 浙江大学 | Structure photogenerated apparatus and method based on polarized light phase-modulation |
| CN109459416A (en) * | 2018-11-07 | 2019-03-12 | 天津大学 | The device and method of THz wave imaging signal to noise ratio is improved based on reflection windows |
| CN111670398A (en) * | 2018-02-01 | 2020-09-15 | 莱卡微系统Cms有限责任公司 | Method for imaging a sample by means of a light-sheet microscope |
-
2016
- 2016-09-19 CN CN201621062724.XU patent/CN206115050U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106291962A (en) * | 2016-09-19 | 2017-01-04 | 浙江大学 | Structure photogenerated apparatus and method based on polarized light phase-modulation |
| CN111670398A (en) * | 2018-02-01 | 2020-09-15 | 莱卡微系统Cms有限责任公司 | Method for imaging a sample by means of a light-sheet microscope |
| CN111670398B (en) * | 2018-02-01 | 2023-09-08 | 莱卡微系统Cms有限责任公司 | Method for imaging a sample by means of a light sheet microscope |
| CN109459416A (en) * | 2018-11-07 | 2019-03-12 | 天津大学 | The device and method of THz wave imaging signal to noise ratio is improved based on reflection windows |
| CN109459416B (en) * | 2018-11-07 | 2023-12-22 | 天津大学 | Device and method for improving terahertz wave imaging signal-to-noise ratio based on reflection window |
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