CN206096166U - A negative pressure automatic sample introduction device for micro -fluidic chip - Google Patents
A negative pressure automatic sample introduction device for micro -fluidic chip Download PDFInfo
- Publication number
- CN206096166U CN206096166U CN201621105308.3U CN201621105308U CN206096166U CN 206096166 U CN206096166 U CN 206096166U CN 201621105308 U CN201621105308 U CN 201621105308U CN 206096166 U CN206096166 U CN 206096166U
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- micro
- fluidic chip
- breather
- waste liquid
- screw
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- 239000007788 liquid Substances 0.000 claims abstract description 36
- 239000002699 waste material Substances 0.000 claims abstract description 35
- 238000001514 detection method Methods 0.000 claims abstract description 23
- 238000005070 sampling Methods 0.000 claims description 16
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 9
- 238000002347 injection Methods 0.000 description 5
- 239000007924 injection Substances 0.000 description 5
- 230000005484 gravity Effects 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000002032 lab-on-a-chip Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000003437 trachea Anatomy 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model relates to a negative pressure automatic sample introduction device for micro -fluidic chip, including power supply, actuating mechanism, air -breather, micro -fluidic chip and controller, actuating mechanism connects in the output of power supply, and actuating mechanism's execution end is connected with the air -breather, last detection zone, waste liquid pool and a plurality of sample cell of being equipped with of micro -fluidic chip, every sample cell are linked together with the detection zone through microfluidic channel respectively, and the waste liquid pool is linked together with the detection zone through microfluidic channel, the air -breather includes breather pipe, normally -closed solenoid valves, tee bend, negative -pressure vacuum pump and vacuum chuck, tee junction in actuating mechanism's execution end, and vacuum chuck is connected to one end, and the other end passes through the breather pipe and links to each other with negative -pressure vacuum pump, and the third end links to each other with the atmosphere through the breather pipe to be equipped with normally -closed solenoid valves on the breather pipe that links to each other with the atmosphere. The utility model discloses it no longer needs artificial intervention behind micro -fluidic chip's the sample cell to pour into the sample into, wholely advances kind process and is accomplished intact fully intelligent by a controller control key formula.
Description
Technical field
This utility model is related in biology, medical science, chemistry, medicine and other fields entering for the analysis system based on micro-fluidic chip
Sample equipment, specifically a kind of negative pressure automatic sampling apparatus for micro-fluidic chip, is mainly used in realizing micro-fluidic chip
Auto injection.
Background technology
Microflow control technique (Microfluidics) be the sample preparation during biological, chemistry, medical analysis, reaction,
The basic operation units such as separation, detection are integrated into one piece to be had on the chip of micron level passage, complete so as to be automatically performed analysis
Portion's process.Chip carrier used in the process of this is referred to as micro-fluidic chip, is also called " chip lab " (lab-on-a-
chip).Sample introduction process is that analysis sample is injected in micro-fluidic chip, realizes the premise of sequence of operations on chip.Miniflow
The sample introduction of control chip mainly includes various driving means such as electronic, air pressure, centrifugal force, gravity, shearing force, and wherein air pressure drives is
The most frequently used a kind of type of drive, including the promotion using malleation mode and the suction using negative pressure mode.Based on micro-fluidic
In the miniaturization biochemical analyzer of chip, the part that sample introduction process is whole intelligent equipment is automatically performed.
Utility model content
For the sample introduction demand of the Intelligent biochemistry analyser based on micro-fluidic chip, the purpose of this utility model is to provide
A kind of negative pressure automatic sampling apparatus for micro-fluidic chip.Negative pressure driving side of the automatic sampling apparatus using vacuum draw
Formula, the one-touch sample introduction for completing micro-fluidic chip.
The purpose of this utility model is achieved through the following technical solutions：
This utility model includes power source, actuator, breather, micro-fluidic chip and controller, wherein performing machine
Structure is connected to the outfan of power source, and the actuating station of the actuator is connected with breather；Set on the micro-fluidic chip
There are detection zone, waste liquid pool and multiple sample cells, each sample cell is connected with detection zone by microfluidic channel respectively, it is described useless
Liquid pool is connected with detection zone by microfluidic channel；The breather includes breather, normally closed solenoid valve, threeway, negative pressure
Vacuum pump and vacuum cup, the threeway are connected to the actuating station of the actuator, and one end connects the vacuum cup, the other end
It is connected with the negative-pressure vacuum pump by breather, the 3rd end is connected with air by breather, and it is logical what is be connected with air
Trachea is provided with the normally closed solenoid valve；The vacuum cup of the breather covers on the waste liquid pool with actuator movement
On, waste liquid pool is entered after detected by the sample suction detection zone in each sample cell；Institute
State power source, negative-pressure vacuum pump, normally closed solenoid valve and detection zone to be electrically connected with the controller respectively.
Wherein：External diameter of the internal diameter of the vacuum cup more than waste liquid pool, by institute when the vacuum cup is fallen on waste liquid pool
State waste liquid pool to cover, and do not contact with the waste liquid pool；
The actuator is slide block guide rail actuator, including vertical guide rail, slide block A, horizontal guide rail, slide block B and gas
Cylinder B, the power source is cylinder A, and the outfan of cylinder A is connected with slide block A, the slide block A and hanging down on cylinder A
Straight guide is slidably connected, and the horizontal guide rail is provided with cylinder B on the horizontal guide rail on slide block A, the cylinder B's
Outfan is connected with the slide block B being slidably connected with horizontal guide rail, and the threeway is connected in the slide block B；The vertical guide rail and
The position sensor being electrically connected with the controller is separately installed with horizontal guide rail；The threeway is connected with slide block B by gangbar；
The actuator is screw-nut actuator, including leading screw A, screw A, motor B, screw B and leading screw B, institute
Power source is stated for motor A, the outfan of motor A is connected with leading screw A, and the screw A is threadedly coupled with leading screw A, the motor B
On screw A, outfan is connected with the leading screw B, and the screw B is threadedly connected on leading screw B, the threeway connection
On screw B；The position sensor being electrically connected with the controller is separately installed with the leading screw A and leading screw B；Described three all
Cross gangbar to be connected with screw B；
With micro-fluidic chip sealing absorption when the vacuum cup is covered on waste liquid pool.
Advantage of the present utility model with good effect is：
1., relative to the various sample injection methods such as existing malleation, negative pressure, electronic, gravity, advantage of the present utility model is
Manual intervention, whole sample introduction process is no longer needed to control a key by controller after injecting a sample into the sample cell of micro-fluidic chip
Formula is completed, full intellectualized.
2. this utility model adopts Ngatively pressurized sampling, as long as there is one outlet, no matter has several injection ports, all only needs one to bear
Pressure vacuum pump can be completed.
3. this utility model wide adaptability, is adapted to the micro-fluidic chip sample introduction of various channel types.
Description of the drawings
Fig. 1 is structured flowchart of the present utility model；
Fig. 2 is this utility model structural representation；
Fig. 3 is the structural representation of this utility model another kind actuator；
Structural representations of the Fig. 4 for micro-fluidic chip；
Structural representations of the Fig. 5 for this utility model breather；
Fig. 6 is the waste liquid pool interface diagram of the vacuum cup on this utility model breather and micro-fluidic chip；
Wherein：1 is power source, and 2 is actuator, and 201 is vertical guide rail, and 202 is horizontal guide rail, and 203 is slide block A, 204
For position sensor, 205 is gangbar, and 206 is slide block B, and 207 is leading screw A, and 208 is screw A, and 209 is motor B, and 210 is silk
Female B, 211 is leading screw B, and 212 is cylinder B, and 3 is breather, and 301 is breather, and 302 is normally closed solenoid valve, and 303 is threeway,
304 is negative-pressure vacuum pump, and 305 is vacuum cup, and 4 is micro-fluidic chip, and 401 is microfluidic channel, and 402 is waste liquid pool, and 403 are
Sample cell, 404 is detection zone.
Specific embodiment
The utility model is described in further detail below in conjunction with the accompanying drawings.
As shown in Figure 1, Figure 2 and shown in Fig. 4, this utility model includes power source 1, actuator 2, breather 3, micro-fluidic core
Piece 4 and controller, wherein actuator 2 are connected to the outfan of power source 1, are connected with ventilation dress in the actuating station of actuator
Put 3；Micro-fluidic chip 4 is provided with detection zone 404, waste liquid pool 402 and multiple sample cells 403, and each sample cell 403 passes through respectively
Microfluidic channel 401 is connected with detection zone 404, and waste liquid pool 402 is connected with detection zone 404 by microfluidic channel 401.It is micro-
Fluidic chip 4 can have multiple injection ports, and each injection port one sample cell 403 of correspondence, sample are manually injected into sample cell 403
Waste liquid pool 402 is entered after detection zone 404 by negative pressure-pumping.
As shown in Figure 5, Figure 6, breather 3 includes breather 301, normally closed solenoid valve 302, threeway 303, negative-pressure vacuum pump
304 and vacuum cup 305, the top of the threeway 303 is connected to the actuating station of actuator, one end connection vacuum cup 305, separately
One end is connected with negative-pressure vacuum pump 304 by breather 301, and the 3rd end is connected with air by breather 301, and with air
Connected breather 301 is provided with normally closed solenoid valve 302.The internal diameter of vacuum cup 305 is more than waste liquid pool on micro-fluidic chip 4
402 external diameter, when vacuum cup 305 is fallen on waste liquid pool 402, it is ensured that waste liquid pool 402 is covered by vacuum cup 305,
And do not contact with waste liquid pool 402, so as to avoid cross-contamination.Vacuum cup of the present utility model 305 is resilient for tool
Silica gel material, when vacuum cup 305 is covered on waste liquid pool 402 with the sealing absorption of micro-fluidic chip 4, can play close well
Envelope is acted on, it is ensured that enough vacuum realizes Ngatively pressurized sampling.
As shown in Fig. 2 actuator 2 is slide block guide rail actuator, including vertical guide rail 201, slide block A203, level are led
Rail 202, slide block B 206, position sensor 204, gangbar 205 and cylinder B212, power source 1 be cylinder A, the output of cylinder A
End is connected with slide block A203, and slide block A203 is slidably connected with the vertical guide rail 201 on cylinder A；Horizontal guide rail 202 is installed
On slide block A203, cylinder B212 is provided with the horizontal guide rail 202, the outfan of cylinder B212 is connected with and horizontal guide rail
202 slide block Bs 206 being slidably connected, the top of threeway 303 are connected in the slide block B 206 by gangbar 205.In vertical guide rail
201 and horizontal guide rail 202 on be separately installed with the position sensor 204 being electrically connected with the controller.
Actuator of the present utility model 2 is not limited to guide rail motion, also can be as shown in figure 3, actuator 2 is screw-nut
Actuator, including leading screw A207, screw A208, motor B209, screw B210, position sensor 204, gangbar 205 and silk
Thick stick B211, power source 1 is motor A, and the outfan of motor A is connected with leading screw A207, screw A208 and leading screw A207 screw thread and connects
Connect, on screw A208, outfan is connected with leading screw B211 to motor B209, and screw B210 is threadedly connected to leading screw B211
On, the top of threeway 303 is connected on screw B210 by gangbar 205.Pacify on leading screw A207 and leading screw B211 respectively
Equipped with the position sensor 204 being electrically connected with the controller.
Or, actuator can also be rotary motion, drive breather to reach the waste liquid pool 402 on micro-fluidic chip
Place.
Power source of the present utility model 1, negative-pressure vacuum pump 304, normally closed solenoid valve 302, detection zone 404 and position sensor
204 are electrically connected with the controller respectively.
Operation principle of the present utility model is：
So that actuator 2 is slide block guide rail actuator as an example.
Controller (controller of the present utility model is as prior art) control with embedded microprocessor as core is whole
Automatic sampling apparatus.After controller sends sample introduction to be instructed, cylinder A work, band movable slider A203 are moved on vertical guide rail 201
Dynamic, with slide block A203 synchronizing movings, the cylinder B212 on horizontal guide rail 202 drives slide block B 206 to lead in level to horizontal guide rail 202
(can first vertically move and move horizontally afterwards, or first move horizontally vertically move afterwards) is moved on rail 202, by 205 He of gangbar
Breather 3 is connected, and makes breather 3 reach specified location by controlling the horizontally or vertically motion of actuator 2, and position passes
Sensor 204 feeds back stop motion signal to controller, and controller control actuator 2 stops movement, and makes on breather 3
At the waste liquid pool 402 that vacuum cup 305 just falls on micro-fluidic chip 4, and waste liquid pool 402 is covered.Controller starts logical
Negative-pressure vacuum pump 304 in device of air 3 works, and in the presence of negative pressure, vacuum cup 305 tightly holds micro-fluidic chip 4, and
Starting to aspirate makes sample enter into waste liquid pool Jing after microfluidic channel 401 flows through detection zone 404 from the sample cell 403 of import
402.After sample is all flowed into waste liquid pool 402, the detection zone sensors detection sample introduction end signal of micro-fluidic chip 4 is simultaneously anti-
Controller of feeding prompting sample introduction is completed, and controller sends instruction stopping negative-pressure vacuum pump 304 and works, and opens breather 3
Normally closed solenoid valve 302 admits air into release vacuum cup 305 in threeway 303, makes vacuum cup 305 leave micro-fluidic core
Piece.Subsequently, controller control actuator 2 drives breather 3 to leave waste liquid pool 402, returns to original position, completes once to enter
Sample process.
The targeted micro-fluidic chip of this utility model is not limited to configuration shown in Fig. 4, for other configurations are with one
Outlet, multiple entrances micro-fluidic chip it is equally applicable.
Claims (9)
1. a kind of negative pressure automatic sampling apparatus for micro-fluidic chip, it is characterised in that：Including power source (1), actuator
(2), breather (3), micro-fluidic chip (4) and controller, wherein actuator (2) are connected to the outfan of power source (1),
The actuating station of the actuator is connected with breather (3)；The micro-fluidic chip (4) is provided with detection zone (404), waste liquid
Pond (402) and multiple sample cells (403), each sample cell (403) is respectively by microfluidic channel (401) and detection zone (404)
It is connected, the waste liquid pool (402) is connected with detection zone (404) by microfluidic channel (401)；The breather (3)
Including breather (301), normally closed solenoid valve (302), threeway (303), negative-pressure vacuum pump (304) and vacuum cup (305), this three
Logical (303) are connected to the actuating station of the actuator, and one end connects the vacuum cup (305), and the other end passes through breather
(301) it is connected with the negative-pressure vacuum pump (304), the 3rd end is connected with air by breather (301), and is being connected with air
Breather (301) be provided with the normally closed solenoid valve (302)；The vacuum cup (305) of the breather (3) is with the machine of execution
Structure (2) movement is covered on the waste liquid pool (402), by the negative-pressure vacuum pump (304) by each sample cell (403)
Sample suction detection zone (404) enters waste liquid pool (402) after being detected；The power source (1), negative-pressure vacuum pump (304), often
Close electromagnetic valve (302) and detection zone (404) is electrically connected with the controller respectively.
2. the negative pressure automatic sampling apparatus of micro-fluidic chip are used for as described in claim 1, it is characterised in that：The vacuum cup
(305) external diameter of the internal diameter more than waste liquid pool (402), by the waste liquid when vacuum cup (305) is fallen on waste liquid pool (402)
Pond (402) covers, and does not contact with the waste liquid pool (402).
3. the negative pressure automatic sampling apparatus of micro-fluidic chip are used for as described in claim 1 or 2, it is characterised in that：The execution
Mechanism (2) is slide block guide rail actuator, including vertical guide rail (201), slide block A (203), horizontal guide rail (202), slide block B
(206) and cylinder B (212), the power source (1) is cylinder A, and the outfan of cylinder A is connected with slide block A (203), the cunning
Block A (203) is slidably connected with the vertical guide rail (201) on cylinder A, and the horizontal guide rail (202) is installed in slide block A
(203) on, cylinder B (212) is provided with the horizontal guide rail (202), the outfan of the cylinder B (212) is connected with and level
The slide block B (206) that guide rail (202) is slidably connected, the threeway (303) are connected in the slide block B (206).
4. the negative pressure automatic sampling apparatus of micro-fluidic chip are used for as described in claim 3, it is characterised in that：The vertical guide rail
(201) and on horizontal guide rail (202) it is separately installed with the position sensor (204) being electrically connected with the controller.
5. the negative pressure automatic sampling apparatus of micro-fluidic chip are used for as described in claim 3, it is characterised in that：The threeway
(303) it is connected with slide block B (206) by gangbar (205).
6. the negative pressure automatic sampling apparatus of micro-fluidic chip are used for as described in claim 1 or 2, it is characterised in that：The execution
Mechanism (2) is screw-nut actuator, including leading screw A (207), screw A (208), motor B (209), screw B (210) and silk
Thick stick B (211), the power source (1) is motor A, and the outfan of motor A is connected with leading screw A (207), the screw A (208)
It is threadedly coupled with leading screw A (207), on screw A (208), outfan is connected with the leading screw B to the motor B (209)
(211), the screw B (210) is threadedly connected on leading screw B (211), and the threeway (303) is connected on screw B (210).
7. the negative pressure automatic sampling apparatus of micro-fluidic chip are used for as described in claim 6, it is characterised in that：The leading screw A
(207) and on leading screw B (211) it is separately installed with the position sensor (204) being electrically connected with the controller.
8. the negative pressure automatic sampling apparatus of micro-fluidic chip are used for as described in claim 6, it is characterised in that：The threeway
(303) it is connected with screw B (210) by gangbar (205).
9. the negative pressure automatic sampling apparatus of micro-fluidic chip are used for as described in claim 1 or 2, it is characterised in that：The vacuum
With micro-fluidic chip (4) sealing absorption when sucker (305) is covered on waste liquid pool (402).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621105308.3U CN206096166U (en) | 2016-10-09 | 2016-10-09 | A negative pressure automatic sample introduction device for micro -fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621105308.3U CN206096166U (en) | 2016-10-09 | 2016-10-09 | A negative pressure automatic sample introduction device for micro -fluidic chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206096166U true CN206096166U (en) | 2017-04-12 |
Family
ID=58482902
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621105308.3U Withdrawn - After Issue CN206096166U (en) | 2016-10-09 | 2016-10-09 | A negative pressure automatic sample introduction device for micro -fluidic chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206096166U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106370878A (en) * | 2016-10-09 | 2017-02-01 | 戴敬 | Negative pressure automatic sampling device for microfluidic chip |
| CN110538680A (en) * | 2019-08-19 | 2019-12-06 | 昆山汇先医药技术有限公司 | Micro-fluidic sample processing equipment |
-
2016
- 2016-10-09 CN CN201621105308.3U patent/CN206096166U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106370878A (en) * | 2016-10-09 | 2017-02-01 | 戴敬 | Negative pressure automatic sampling device for microfluidic chip |
| CN110538680A (en) * | 2019-08-19 | 2019-12-06 | 昆山汇先医药技术有限公司 | Micro-fluidic sample processing equipment |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180622 Address after: 215123 9 Tengfei Science Park, 388 Xinping street, Suzhou Industrial Park, Suzhou, Jiangsu. Co-patentee after: Suzhou painted real Biology Technology Co., Ltd. Patentee after: Suzhou industry park dragon biological science and Technology Research Institute Co Ltd Co-patentee after: Suzhou de SAP Biological Technology Co., Ltd. Address before: 110179 hunhunwan District, Hunnan New District, Shenyang, Liaoning 3, No. 3-17, Hunhe Bay, 3-17 Patentee before: Dai Jing |
|
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20170412 Effective date of abandoning: 20180918 |