CN206089712U - Whole blood peptide powder extraction element - Google Patents
Whole blood peptide powder extraction element Download PDFInfo
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- CN206089712U CN206089712U CN201620870483.5U CN201620870483U CN206089712U CN 206089712 U CN206089712 U CN 206089712U CN 201620870483 U CN201620870483 U CN 201620870483U CN 206089712 U CN206089712 U CN 206089712U
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Abstract
The utility model relates to a whole blood peptide powder extraction element, especially one kind is drawed the whitewashed method of whole blood peptide with enzyme hydrolysis, ceramic membrane, reverse osmosis method coupling, belongs to biotechnology enzymolysis whole blood albumen and membrane separation technique field. Including: enzymolysis tank (1) for it handles to carry out the enzymolysis to livestock blood, solid -liquid separation equipment (8) for enzymolysis liquid to obtaining among the enzymolysis tank carries out solid -liquid separation disposal, milipore filter (4) for the edulcoration is filtered to feed liquid to behind the solid -liquid separation, reverse osmosis membrane (6) for penetrant to milipore filter (4) carries out the concentration processing. The device can obtain protein in the livestock blood effectively to draw the acid soluble protein content of product, the extraction efficiency is high, can isolate polypeptide and amino acid simultaneously, has realized cleaner production, has reduced area.
Description
Technical field
The utility model is related to a kind of whole blood Gly-His-Lys extraction element, particularly a kind of by enzyme hydrolysis, ceramic membrane, hyperfiltration
The method that whole blood Gly-His-Lys are extracted in coupling, belongs to biotechnology enzymolysis whole blood albumen and technical field of membrane separation.
Background technology
At present, of paramount importance albumen source is fish meal in China's feed.Gly-His-Lys as a kind of good feed addictive,
Per kilogram can produce the kilocalorie of digestible energy 41, and rich in protein, fat and various trace elements, nutrient content is comprehensive.Chicken, pig,
Add Gly-His-Lys in the animal and fowl fodders such as ox, growth of animals or poultry can be promoted, shorten breeding cycle.Gly-His-Lys animal digestion rate relies ammonia up to 90%
Acid is 7%～8%, is, leucine content 8% or so higher than fish meal, digested tankage content first of all natural feeds.In addition, livestock
Blood accounts for body weight 8%, and the recovery ratio of blood accounts for the 3%～5% of body weight, for the whole nation, can be used to process profit
Livestock blood is a huge numeral.Now some areas, can replacing whole using Gly-His-Lys and the mutual compatibility of feather meal
High-quality fish meal.
Chinese patent CN101843289B discloses a kind of use enzymatic isolation method and whole blood polypeptide albumen powder is extracted from livestock blood
Method, by selecting suitable substrates concentration, proteolysis complex enzyme and reaction temperature, makes livestock blood pass through enzymolysis, reduction, be dried
Whole blood polypeptide albumen powder is obtained with crushing.Patent CN101263861B discloses a kind of method of extraction annimal hydrolyzed protein powder,
Annimal hydrolyzed protein powder is obtained after enzyme hydrolysis, filtration, evaporation and concentration, drying.Although the protein one that above-mentioned method is extracted
Determine degree and remain its biologically active, but filtering accuracy is not high, therefore product purity is not high, additionally, adopted condensing mode is equal
To be concentrated by evaporation, the removal of the salts substances in high energy consumption and product is studied.Therefore a kind of economically feasible is developed, is produced
The high easily absorbed method of protein of product purity.
Utility model content
The purpose of this utility model is to provide a kind of whole blood Gly-His-Lys extraction element, needs to improve and extracts purity of protein, increases
The content of sour molten albumen in finished product, and it is simple to operate, pollute it is little, can realize clean manufacturing, suitable for large-scale production.Mainly
Purified concentration operation is carried out by ceramic membrane ultrafitration+reverse osmosis membrane integrated technology, the concrete technical scheme of employing is as follows：
A kind of whole blood Gly-His-Lys extracting method, comprises the steps：
1st step, in livestock blood add enzyme digested；
2nd step, enzymolysis liquid carry out separation of solid and liquid process；
The feed liquid that 3rd step, separation of solid and liquid are obtained carries out filtering and impurity removing by milipore filter；
4th step, the permeate of milipore filter are again through reverse osmosis membrane concentration.
Described livestock blood is the blood obtained after the butchering of pig, ox, sheep, horse, donkey, chicken, duck, goose etc..
In the 1st described step, it is trypsase, pancreatinum, chymotrypsin, stomach cardia to digest the ferment for using
In the protein decomposition enzymes such as enzyme, papain, kallikrein, cathepsin, thermolysin or V8 protease
One or several combination；55～70 DEG C of hydrolysis temperature, digests pH7～9, enzymolysis time 2～2.5 hours.
In the 2nd described step, the mode of separation of solid and liquid selected from centrifugation mode, expression separation mode, filter type, on
One or several combination in floating separate mode, sedimentation separation mode；It is preferred that first using coarse filter again using centrifugation
Separated.
In the 3rd described step, the 1000～200000Da of molecular cut off of described milipore filter, the material of milipore filter is selected from
Cellulose, cellulose esters, polysulfones, polyether sulfone, polyvinyl chloride, chloropropene, polyolefin, polyvinyl alcohol, polymethyl methacrylate,
One or several combination in polyvinylidene fluoride, polytetrafluoroethylene (PTFE), ceramics.
In the 3rd described step, described milipore filter is ceramic super-filtering film, and the scope of average pore size is 50～200nm, cross-film
Pressure differential range is 0.1～0.3MPa, and 2～5m/s of crossflow velocity, ultra-filtration filters temperature is preferably 40～60 DEG C, and multiple is concentrated by ultrafiltration
It is 3～4 times.
In the 4th described step, the material of reverse osmosis membrane is sub- selected from birds of the same feather flock together compound, polyamide, polyester, polyamides of cellulose acetate
One or several combination in amine or polyvinyl etc.；Reverse osmosis process preferred parameter is：Operating pressure is 2.5
～3.0MPa, circular flow is 2.0～3.0m3/ h, cycles of concentration is 2～3 times.
In one embodiment, the concentrate drying that reverse osmosis membrane is obtained is processed；Dried process is preferably using spray
Mist is dried.
In one embodiment, the permeate of milipore filter is after electrodialysis desalination, be re-fed into reverse osmosis membrane carry out it is dense
Contracting.The preferred operational factor of electrodialysis desalination is：Electrodialysis operation voltage is 100～200V, 1～3A of electric current, and feed pressure is
0.05～0.2MPa.
A kind of whole blood Gly-His-Lys extraction element, includes：
Enzymatic vessel, for carrying out enzymolysis processing to livestock blood；
Equipment for separating liquid from solid, for carrying out separation of solid and liquid process to the enzymolysis liquid obtained in enzymatic vessel；
Milipore filter, for carrying out filtering and impurity removing to the feed liquid after separation of solid and liquid；
Reverse osmosis membrane, for carrying out concentration to the penetrating fluid of milipore filter 4.
Described equipment for separating liquid from solid is filled selected from centrifugal separating device, expression separation device, filter, separation of floating
Put, one or several the component in settling separation device；It is preferred that first using the combination of centrifugal separating device and coarse filter,
Centrifugal separating device is used to carry out enzymolysis liquid centrifugal treating, and the mother liquor that coarse filter is used to obtain centrifugal separating device is carried out
Filtering and impurity removing process.
1000～the 200000Da of molecular cut off of described milipore filter, the material of milipore filter is selected from cellulose, cellulose
Ester, polysulfones, polyether sulfone, polyvinyl chloride, chloropropene, polyolefin, polyvinyl alcohol, polymethyl methacrylate, polyvinylidene fluoride,
One or several combination in polytetrafluoroethylene (PTFE), ceramics.
Described milipore filter is ceramic super-filtering film, and the scope of average pore size is 50～200nm.
The material of reverse osmosis membrane selected from cellulose acetate birds of the same feather flock together compound, polyamide, polyester, polyimides or vinyl gather
One or several combination in compound etc..
In one embodiment, drying device is also included, for being dried process to the concentrate of reverse osmosis membrane.
Drying device is spray-drying installation.
In one embodiment, electrodialysis plant is also included in device, for being concentrated into reverse osmosis membrane
The penetrating fluid of milipore filter 4 carries out desalting processing.
Enzyme is also included in described device and adds part, for adding enzyme in livestock blood.
Beneficial effect
The utility model provides a kind of method that membrane separation technique extracts whole blood Gly-His-Lys, using enzyme hydrolysis and ceramic membrane+
Hyperfiltration is coupled, and simple to operate, recovery rate is high, and energy consumption is low, realizes clean manufacturing, reduces floor space.Ceramic excessive filtration
Process greatly mitigates the pollution of electrodialysis desalination system and counter-infiltration system, and counter-infiltration system substitutes traditional economic benefits and social benefits and is concentrated by evaporation energy
Substantially reduce energy consumption, improving product quality, it is adaptable to industrial production.
Description of the drawings
Fig. 1 is installation drawing of the present utility model.
Specific embodiment
The utility model is described in further detail below by specific embodiment.But those skilled in the art will
Understand, the following example is merely to illustrate the utility model, and should not be regarded as limiting scope of the present utility model.In embodiment not
Dated particular technique or condition person, enter according to the technology or condition described by document in the art or according to product description
OK.Agents useful for same or the unreceipted production firm person of instrument, be can pass through city available from conventional products.
Using range format expression value should be interpreted as in a flexible way not only include clearly include as scope
The numerical value of limit value, but also including all single numbers or subinterval for covering within the range, just as each numerical value and sub-district
Between be expressly recited out.For example, the concentration range of " about 0.1% to about 5% " should be understood to not only include clearly including
About 0.1% to about 5% concentration, also including in the range of alluding to single concentration (e.g., 1%, 2%, 3% and 4%) and son
Interval (for example, 0.1% to 0.5%, 1% to 2.2%, 3.3% to 4.4%).Percentage described in the utility model is in nothing
In the case of special instruction, percentage by weight is referred to.
" one embodiment ", " another embodiment ", " embodiment " for addressing in this manual etc., refer to
Specific features, structure with reference to embodiment description are included at least one embodiment of the application generality description.
It is not necessarily to refer to same embodiment that in the description statement of the same race occur in multiple places.Furthermore, it is understood that with reference to arbitrary
When embodiment describes specific features, structure or a feature, what is advocated is realizing this spy with reference to other embodiment
Levy, structure or feature also fall in the application scope of the claimed.
The extracting method first step of the present utility model is that the effect by digesting digests protein macromolecule in livestock blood,
Make it be more easy to be separated, purified and be more easy to animal absorption, further, since can produce in enzymolysis process a part of polypeptide and
Amino acid, simultaneously containing a large amount of impurity, the polypeptide and amino acid in this part of hydrolysate is sour molten albumen in finished product Gly-His-Lys
Main constituents, need to separate it from protein, improve the quality of product, and the utility model to enzymolysis liquid by carrying out
Solid-liquid initial gross separation, the detached process of ultrafiltration precision are by Impurity removal, then are extracted by reverse osmosis concentration, drying.Hydrolysis
The parameter of technique includes the type of enzyme, enzymolysis pH, hydrolysis temperature and enzymolysis time etc., and the difference of enzymolysis process influences whether egg
The Degree of Enzymatic Hydrolysis of white matter, the technological parameter of ultrafiltration, yield of product etc..If Degree of Enzymatic Hydrolysis is higher, protein can be caused excessive
Be hydrolyzed and yield is low；If conversely, Degree of Enzymatic Hydrolysis not enough, can cause protein to be unable to complete hydrolysis, more albumen
Matter can produce pollution to milipore filter.More preferably digesting parameter is：The ferment that uses of enzymolysis be trypsase, pancreatinum,
Chymotrypsin, pepsin, papain, kallikrein, cathepsin, thermolysin and V8 protease
Deng protein decomposition enzyme；55～70 DEG C of hydrolysis temperature, digests pH7～9, enzymolysis time 2～2.5 hours.
Livestock blood described in the utility model, is not particularly limited, as long as the blood of the livestock for generally cultivating is all
It is achieved that for example：The blood obtained after the butchering of pig, ox, sheep, horse, donkey, chicken, duck, goose etc..
After enzymolysis liquid has been obtained, larger solids therein can be gone by way of separation of solid and liquid
Remove, the mode of separation of solid and liquid here is not particularly limited.As the method for specific separation of solid and liquid process, centrifugation can be enumerated
Separate mode, expression separation mode, filter type, floating separate mode, sedimentation separation mode.As centrifugation mode, can
To illustrate horizontal continuous centrifuge (process of spiral decanter), separate board-like centrifugal separator, filter centrifugal, the general Le in tall building
This type UCF ultracentrifuge, as filter type, can illustrate band filter, belt press, flypress, precoat filter,
Filter press, as floating separate mode, can illustrate continuous floating separator, as sedimentation separation mode, can illustrate solidifying
Collection sedimentation separation machine, rapid sedimentation separation machine etc., but it is not particularly limited in above-mentioned any one.But above-mentioned appointing can be passed through
One or its combination are reducing when secondary filter film and/or ultrafiltration membrane treatment to the load of film.More preferably mode is to first pass through
Centrifugation goes out solids, then with coarse filter after filtration, is re-fed into carrying out filtering and impurity removing to follow-up milipore filter.Centrifugation
Parameter is preferably：2500～3000 revs/min of mair motor, 1000 revs/min of auxiliary motor.In 3rd step, hydrolysate firstly the need of
Large granular impurity therein is removed by coarse filter, this is mainly livestock hair, skin etc., the pollution of milipore filter can be mitigated, carried
High product purities, coarse filter can be conventional sheet frame, video disc etc..
Milipore filter used in the utility model, be molecular cut off be 1000～200000 film, referred to as ultrafiltration
Film, UF films etc..Here, being difficult to determine the aperture on film surface with electron microscope etc. because the aperture of milipore filter is too small, institute
To replace average pore size to be used as the index of pore size with the value of referred to as molecular cut off.With regard to molecular cut off, such as at this
Described in the handbook of field：" will with molecular weight solute as transverse axis, prevention rate as the longitudinal axis, the curve for carrying out drawing to data
Referred to as molecular cut off curve.And the molecular weight that prevention rate is 90% is referred to as into the molecular cut off of film ", molecular cut off is made
To represent the index of the film properties of milipore filter, it is well known to those skilled in the art.
As the material of these milipore filters, as long as can realize removing as above-mentioned water soluble polymer and colloid composition
The utility model purpose, is not particularly limited, and can enumerate：Cellulose, cellulose esters, polysulfones, polyether sulfone, polychlorostyrene second
The organic materials such as alkene, chloropropene, polyolefin, polyvinyl alcohol, polymethyl methacrylate, polyvinylidene fluoride, polytetrafluoroethylene (PTFE),
Or the inorganic material such as metal or ceramics such as stainless steel.The material of milipore filter can consider the proterties of zymolyte or operation
Into original appropriate selection, consider from the service life of film, the milipore filter of preferably ceramic material.As many of composition ceramic separation film
The material of pore membrane, can suitably select from existing known ceramic material.It is, for example possible to use aluminum oxide, zirconium oxide, oxidation
The oxide materials such as magnesium, silica, titanium oxide, cerium oxide, yittrium oxide, barium titanate；Cordierite, mullite, magnesium olive
The combined oxidation species material such as stone, steatite, sialon, zircon, ferrite；Silicon nitride, aluminum nitride and other nitride class
Material；The carbon compound material such as carborundum；The hydroxide composition material such as hydroxyapatite；The element class material such as carbon, silicon；Or contain
There are their two or more inorganic composite materials etc..Can also using natural minerals (clay, clay mineral, cry loudly slag, silica sand,
Pottery stone, feldspar, white sand) or blast-furnace slag, flying dust etc..Wherein, be preferably selected from aluminum oxide, zirconium dioxide, titanium oxide, magnesia,
Ceramic powder that is one kind or two or more in silica, more preferably being constituted as main body using aluminum oxide, zirconium dioxide or titanium oxide
End.More than 50 overall mass % of " as main body " wherein, mentioned here expression ceramic powders (more than preferably 75 mass %,
More preferably 80 mass %～100 mass %) it is aluminum oxide or silica.For example, in the porous material, aluminum oxide is relatively inexpensive
And operability is excellent.Further, it is possible to be readily formed with the loose structure for being suitable for the detached aperture of liquid, therefore, it is possible to hold
Manufacture of changing places has the ceramic separation film of excellent liquid permeability.Also, in above-mentioned aluminum oxide, particularly preferably using α-oxygen
Change aluminium.Alpha-alumina has the characteristic of stable and fusing point and high mechanical strength in terms of chemistry.Therefore, by using alpha-oxidation
Aluminium, can manufacture the ceramic separation film that can be utilized in broad use (such as industrial circle).
In the operating process of ceramic super-filtering film, to grope through lot of experiments, the scope of the average pore size of milipore filter is 50～
200nm, because aperture is too big, the purity that can cause product declines；Aperture is too little, a part of protein while flux is relatively low
Also it is trapped, product yield is reduced.The material of ceramic super-filtering film is preferably the one kind in aluminum oxide, zirconium oxide, titanium oxide.Super
During filter, the selection of transmembrane pressure suffers from interactional pass with the average pore size of milipore filter, filtration flux, product purity
System, if transmembrane pressure is excessive, per-meate side is reached after a part of protein colloid can be caused pressurized through fenestra, affects product pure
Degree, if transmembrane pressure is too small, can cause filtration flux too small, and cost increases.Groping through lot of experiments, preferably across
Film pressure differential range is 0.1～0.3MPa.Crossflow velocity influences whether filtration flux, if crossflow velocity is excessive, transmembrane pressure meeting
Reduce, filtration flux can be too small, if crossflow velocity is too small, film surface pollution can aggravate, and flux stability can be reduced, preferred model
It is 2～5m/s to enclose.Filtration temperature is preferably 40～60 DEG C, and cocnentration factor is preferably material and concentrates 3～4 times.
After the permeate for obtaining ultrafiltration, can concentration by way of feed liquid is processed so that amino acid and
Polypeptide is concentrated, and here the mode of preferred concentration is concentrated using counter-infiltration, due to counter-infiltration for salt has one
Fixed permeability, passes through can the part inorganic salts in material, so that amino acid and polypeptide are purified.
As the material of reverse osmosis membrane, generally use cellulose acetate and birds of the same feather flock together compound, polyamide, polyester, polyimides, second
Alkenyl polymer Polymer material.In addition, as its construction, having and there is compacted zone at least side of film, from the compacted zone
There is the anisotropic membrane of the minute aperture in the aperture for slowly becoming big to the surface of film inside or opposite side and in the anisotropic membrane
Compacted zone on there is composite membrane of very thin active layer for being formed by other materials etc..Wherein, as the shape of reverse osmosis membrane
Formula, there is macaroni yarn, flat film etc..Generally, it is preferred to the thickness of macaroni yarn peace film is 10 μm～1mm, the external diameter of macaroni yarn is 50 μm
～4mm.In addition, as flat film, preferred anisotropic membrane, as composite membrane preferably by substrate supports such as fabric, braid, non-woven fabrics
Film.But, method of the present utility model is used in which can not rely on the material of reverse osmosis membrane, film construction or form,
It is all effective for any one situation.As representational reverse osmosis membrane, for example, can enumerate cellulose acetate class or polyamide
Class anisotropic membrane, with polyamide-based, polyureas active layer composite membrane etc..Wherein, method of the present utility model is for acetic acid
Cellulose family anisotropic membrane, polyamide-based composite membrane are especially effective.Reverse osmosis membrane assembly is for actually used above-mentioned counter-infiltration
Film and be allowed to the material of shape.In the case that the form of reverse osmosis membrane is flat film, can be incorporated into helical form, tubulose or plate with
Used in the component of frame, in addition, in the case of macaroni yarn, component being incorporated on the basis of bundles of and be used.
The utility model is suitable in which can not rely on the form of the composition of these reverse osmosis membrane assemblies.Reverse osmosis process preferred parameter is：
Operating pressure is 2.5～3.0MPa, and circular flow is 2.0～3.0m3/ h, cycles of concentration is 2～3 times.Reverse osmosis concentration can be with
Material liquid volume is substantially reduced, mitigates the load that subsequent spray is dried.Grope through many experiments, the choosing of reverse osmosis membrane molecular cut off
50Da～100Da is selected, because containing small molecule amino acid such as lysine, tryptophans in feed liquid, molecular cut off crosses conference and makes yield
Reduce；Molecular cut off is too small to increase can energy consumption.
After reverse osmosis concentrated liquid has been obtained, pressed powder can be obtained by way of other dryings, for example may be used
To be processed reverse osmosis concentrated liquid using spray dried form, the operating time is short, it is ensured that protein is in dry mistake
Will not be destroyed in journey, obtain the Gly-His-Lys containing polypeptide and amino acid.
In one embodiment, ultrafiltration permeate can be carried out after desalting processing using electrodialysis, is re-fed into reverse osmosis
Permeable membrane is concentrated, and electrodialysis with good desalting effect, shows because operating condition is gentle, without phase transformation and the features such as easily amplify
Work improves product quality, it is possible to improve the rejection of reverse osmosis membrane, and preferred operational factor is：Electrodialysis operation voltage is
100～200V, 1～3A of electric current, feed pressure is 0.05～0.2MPa.
The device that the utility model is provided is as shown in figure 1, include：
Enzymatic vessel 1, for carrying out enzymolysis processing to livestock blood；
Equipment for separating liquid from solid 8, for carrying out separation of solid and liquid process to the enzymolysis liquid obtained in enzymatic vessel；
Milipore filter 4, for carrying out filtering and impurity removing to the feed liquid after separation of solid and liquid；
Reverse osmosis membrane 6, for carrying out concentration to the penetrating fluid of milipore filter 4；
Drying device 7, for being dried process to the concentrate of reverse osmosis membrane 6.
In one embodiment, centrifugal separating device 2 and coarse filter 3, centrifugation are included in equipment for separating liquid from solid 8
Device 2 is used to carry out enzymolysis liquid centrifugal treating, and coarse filter 3 is used to filter the mother liquor that centrifugal separating device 2 is obtained
Removal of impurities is processed.
In one embodiment, electrodialysis plant 5 is also included in device, for concentrating into reverse osmosis membrane 6
The penetrating fluid of milipore filter 4 carry out desalting processing.
In one embodiment, drying device 7 is spray-drying installation.
Embodiment 1
Take the livestock blood 200kg that pig slaughtering factory obtains and pump into enzymatic vessel, be passed through steam and be warming up to 55 DEG C, add tryptose
Enzyme 100g is digested, and it is 7 to control pH, digests 2h, 65 DEG C of hydrolysis temperature.Enzymolysis liquid feeding centrifuge is centrifuged, main electricity
2500 revs/min of machine, 1000 revs/min of auxiliary motor.Centrifuge mother liquor sends into sheet frame and carries out entering ceramic super-filtering film, pottery after press filtration
It is respectively that using 20nm, 50nm, 200nm, 500nm, material is aluminum oxide, ultra-filtration process cross-film that the average pore size of porcelain milipore filter is
Pressure reduction 0.1Mpa, crossflow velocity 2m/s, 40 DEG C of filtration temperature stops after feed liquid is concentrated into 3 times.Ultrafiltration dope is returned into enzymatic vessel
Continue to digest, the penetrating fluid of ultrafiltration is sent into reverse osmosis membrane and concentrated, and operating pressure is 2MPa, and circular flow is 3.0m3/h, dense
Demagnification number is 3 times, obtains the mixture of polypeptide and amino acid.Result of the test at different conditions is as follows：3
| Ceramic membrane average pore size | 20nm | 50nm | 200nm | 500nm |
| Peptide content g/100g | 12.4 | 14.1 | 13.8 | 11.4 |
| Sour molten total amino acid content g/100g in Gly-His-Lys | 15.4 | 16.3 | 16.4 | 14.2 |
| Reverse osmosis membrane amino acid rejection % | 95.4 | 96.3 | 96.5 | 94.2 |
| Gly-His-Lys number-average molecular weight | 513 | 564 | 789 | 2432 |
| Gly-His-Lys weight average molecular weight | 538 | 623 | 884 | 2682 |
| VBN mg/100g | 72 | 70 | 84 | 96 |
| Fatty % | 0.9 | 0.8 | 1.3 | 1.5 |
Embodiment 2
Take the livestock blood 200kg that pig slaughtering factory obtains and pump into enzymatic vessel, be passed through steam and be warming up to 55 DEG C, add tryptose
Enzyme 100g is digested, and it is 7 to control pH, digests 2h, 65 DEG C of hydrolysis temperature.Enzymolysis liquid feeding centrifuge is centrifuged, main electricity
2500 revs/min of machine, 1000 revs/min of auxiliary motor.Centrifuge mother liquor sends into sheet frame and carries out entering ceramic super-filtering film, pottery after press filtration
The average pore size of porcelain milipore filter is 50nm, and material is aluminum oxide, ultra-filtration process transmembrane pressure is respectively 0.1,0.2,0.3,0.4,
0.5Mpa, crossflow velocity 2m/s, 40 DEG C of filtration temperature stops after feed liquid is concentrated into 3 times.Ultrafiltration dope is returned into enzymatic vessel to continue
Enzymolysis, the penetrating fluid of ultrafiltration is sent into reverse osmosis membrane and is concentrated, and operating pressure is 2MPa, and circular flow is 3.0m3/h, concentration times
Number is 3 times, obtains the mixture of polypeptide and amino acid.Result of the test at different conditions is as follows：3
| Ceramic membrane transmembrane pressure MPa | 0.1 | 0.2 | 0.3 | 0.4 | 0.5 |
| Peptide content g/100g | 14.1 | 14.3 | 14.5 | 13.2 | 13.6 |
| Sour molten total amino acid content g/100g in Gly-His-Lys | 16.3 | 16.4 | 15.6 | 15.3 | 15.1 |
| Reverse osmosis membrane amino acid rejection % | 96.3 | 96.5 | 95.6 | 95.7 | 95.1 |
| Gly-His-Lys number-average molecular weight | 564 | 532 | 635 | 754 | 891 |
| Gly-His-Lys weight average molecular weight | 623 | 565 | 681 | 795 | 962 |
| VBN mg/100g | 70 | 72 | 86 | 84 | 88 |
| Fatty % | 0.8 | 0.7 | 0.9 | 1.1 | 1.1 |
Embodiment 3
Take the livestock blood 200kg that pig slaughtering factory obtains and pump into enzymatic vessel, be passed through steam and be warming up to 55 DEG C, add tryptose
Enzyme 80g is digested, and it is 7 to control pH, digests 3h, 60 DEG C of hydrolysis temperature.Enzymolysis liquid feeding centrifuge is centrifuged, mair motor
2000 revs/min, 1500 revs/min of auxiliary motor.Centrifuge mother liquor sends into sheet frame and carries out entering ceramic super-filtering film, ceramics after press filtration
The average pore size of milipore filter is 200nm, and material is aluminum oxide, and ultra-filtration process transmembrane pressure is respectively 0.3Mpa, crossflow velocity 2m/
S, 35 DEG C of filtration temperature stops after feed liquid is concentrated into 4 times.Ultrafiltration dope is returned into enzymatic vessel to continue to digest, the penetrating fluid of ultrafiltration
Send into reverse osmosis membrane to be concentrated, operating pressure is respectively 1.5,2.0,2.5,3.0MPa, circular flow is 2.5m3/h, concentration
Multiple is 3.5 times, obtains the mixture of polypeptide and amino acid.Result of the test at different conditions is as follows：3
| Counter-infiltration operates MPa | 1.5 | 2.0 | 2.5 | 3.0 |
| Peptide content g/100g | 13.6 | 12.4 | 14.1 | 13.8 |
| Sour molten total amino acid content g/100g in Gly-His-Lys | 16.5 | 15.4 | 16.3 | 16.4 |
| Reverse osmosis membrane amino acid rejection % | 95.4 | 96.9 | 96.8 | 97.1 |
| Gly-His-Lys number-average molecular weight | 768 | 786 | 795 | 826 |
| Gly-His-Lys weight average molecular weight | 779 | 854 | 815 | 849 |
| VBN mg/100g | 75 | 83 | 79 | 80 |
| Fatty % | 1.1 | 1.2 | 1.1 | 1.2 |
Embodiment 4
Take the livestock blood 200kg that pig slaughtering factory obtains and pump into enzymatic vessel, be passed through steam and be warming up to 55 DEG C, add tryptose
Enzyme 100g is digested, and it is 7 to control pH, digests 2h, 65 DEG C of hydrolysis temperature.Enzymolysis liquid feeding centrifuge is centrifuged, main electricity
2500 revs/min of machine, 1000 revs/min of auxiliary motor.Centrifuge mother liquor sends into sheet frame and carries out entering ceramic super-filtering film, pottery after press filtration
It is respectively that using 20nm, 50nm, 200nm, 500nm, material is aluminum oxide, ultra-filtration process cross-film that the average pore size of porcelain milipore filter is
Pressure reduction 0.1Mpa, crossflow velocity 2m/s, 40 DEG C of filtration temperature stops after feed liquid is concentrated into 3 times.Ultrafiltration dope is returned into enzymatic vessel
Continue to digest, the penetrating fluid of ultrafiltration sends into electrodialysis plant desalination, electrodialysis operation voltage is 150V, electric current 2A, feed pressure
For 0.1MPa, electrodialytic desalinization liquor is sent into reverse osmosis membrane and is concentrated, and operating pressure is 2MPa, and circular flow is 3.0m3/h,
Cycles of concentration is 3 times, obtains the mixture of polypeptide and amino acid.Result of the test at different conditions is as follows：3
| Ceramic membrane average pore size | 20nm | 50nm | 200nm | 500nm |
| Peptide content g/100g | 13.7 | 14.5 | 14.6 | 12.3 |
| Sour molten total amino acid content g/100g in Gly-His-Lys | 16.2 | 16.5 | 16.7 | 14.8 |
| Reverse osmosis membrane amino acid rejection % | 97.2 | 98.7 | 97.7 | 96.6 |
| Gly-His-Lys number-average molecular weight | 502 | 534 | 782 | 2653 |
| Gly-His-Lys weight average molecular weight | 528 | 603 | 852 | 2745 |
| VBN mg/100g | 38 | 37 | 40 | 42 |
| Fatty % | 0.7 | 0.7 | 1.1 | 1.3 |
Embodiment 5
Take the livestock blood 200kg that pig slaughtering factory obtains and pump into enzymatic vessel, be passed through steam and be warming up to 55 DEG C, add tryptose
Enzyme 80g is digested, and it is 9 to control pH, digests 3h, 60 DEG C of hydrolysis temperature.Enzymolysis liquid feeding centrifuge is centrifuged, mair motor
2000 revs/min, 1500 revs/min of auxiliary motor.Centrifuge mother liquor sends into sheet frame and carries out entering ceramic super-filtering film, ceramics after press filtration
The average pore size of milipore filter is 200nm, and material is aluminum oxide, and ultra-filtration process transmembrane pressure is respectively 0.3Mpa, crossflow velocity 2m/
S, 35 DEG C of filtration temperature stops after feed liquid is concentrated into 4 times.Ultrafiltration dope is returned into enzymatic vessel to continue to digest, the penetrating fluid of ultrafiltration
Send into reverse osmosis membrane to be concentrated, operating pressure is respectively 1.5,2.0,2.5,3.0MPa, circular flow is 2.5m3/ h, concentration
Multiple is 3.5 times, obtains the mixture of polypeptide and amino acid.Result of the test at different conditions is as follows.
Claims (10)
1. a kind of whole blood Gly-His-Lys extraction element, it is characterised in that include：
Enzymatic vessel（1）, for carrying out enzymolysis processing to livestock blood；
Equipment for separating liquid from solid（8）, for carrying out separation of solid and liquid process to the enzymolysis liquid obtained in enzymatic vessel；
Milipore filter（4）, for carrying out filtering and impurity removing to the feed liquid after separation of solid and liquid；
Reverse osmosis membrane（6）, for milipore filter（4）Penetrating fluid carry out concentration.
2. whole blood Gly-His-Lys extraction element according to claim 1, it is characterised in that described equipment for separating liquid from solid（8）Choosing
It is a kind of or several from centrifugal separating device, expression separation device, filter, floating separator, settling separation device
The component planted.
3. whole blood Gly-His-Lys extraction element according to claim 2, it is characterised in that described equipment for separating liquid from solid（8）It is
Centrifugal separating device（2）And coarse filter（3）Combination, centrifugal separating device（2）For carrying out centrifugal treating to enzymolysis liquid, slightly
Filter（3）For to centrifugal separating device（2）The mother liquor for obtaining carries out filtering and impurity removing process.
4. whole blood Gly-His-Lys extraction element according to claim 1, it is characterised in that described milipore filter（4）Retention point
Son 1000～200000Da of amount.
5. whole blood Gly-His-Lys extraction element according to claim 1, it is characterised in that milipore filter（4）Material be selected from fiber
It is element, cellulose esters, polysulfones, polyether sulfone, polyvinyl chloride, chloropropene, polyolefin, polyvinyl alcohol, polymethyl methacrylate, poly- inclined
One or several combination in difluoroethylene, polytetrafluoroethylene (PTFE), ceramics.
6. whole blood Gly-His-Lys extraction element according to claim 1, it is characterised in that described milipore filter（4）It is that ceramics are super
Filter membrane, the scope of average pore size is 50～200nm.
7. whole blood Gly-His-Lys extraction element according to claim 1, it is characterised in that reverse osmosis membrane（6）Material be selected from vinegar
Acid cellulose is birdsed of the same feather flock together one or several the group in compound, polyamide, polyester, polyimides or polyvinyl etc.
Close.
8. whole blood Gly-His-Lys extraction element according to claim 1, it is characterised in that also include drying device（7）, it is used for
To reverse osmosis membrane（6）Concentrate be dried process.
9. whole blood Gly-His-Lys extraction element according to claim 8, it is characterised in that drying device（7）It is to be spray-dried dress
Put.
10. whole blood Gly-His-Lys extraction element according to claim 1, it is characterised in that electrodialysis dress is also included in device
Put（5）, for entering reverse osmosis membrane（6）The milipore filter for being concentrated（4）Penetrating fluid carry out desalting processing；Described device
In also include enzyme and add part, for adding enzyme in livestock blood.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106086142A (en) * | 2016-08-12 | 2016-11-09 | 江苏久吾高科技股份有限公司 | A kind of whole blood Gly-His-Lys extracting method and device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106086142A (en) * | 2016-08-12 | 2016-11-09 | 江苏久吾高科技股份有限公司 | A kind of whole blood Gly-His-Lys extracting method and device |
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