CN206082387U - Kit of survey serum glycocholic acid content - Google Patents
Kit of survey serum glycocholic acid content Download PDFInfo
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- CN206082387U CN206082387U CN201621116429.8U CN201621116429U CN206082387U CN 206082387 U CN206082387 U CN 206082387U CN 201621116429 U CN201621116429 U CN 201621116429U CN 206082387 U CN206082387 U CN 206082387U
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Abstract
The utility model discloses a kit of survey serum glycocholic acid content, the on -line screen storage device comprises a base, an upper cover, first reagent bottle and second reagent bottle, be equipped with in the base and be used for first recess, the second recess, third recess and controller, all be equipped with the opening at first reagent bottle and second reagent bottle top, threaded connection has the lid at the opening part, be connected with the motor of locating in the third recess on the controller, it is the double -deck bottle structure of indent structure that two reagent bottles are the bottom, overlap in the epaxial stirring sleeve pipe of the output of motor after all being connected with the skin that stretches out corresponding reagent bottle bottom the inlayer of first reagent bottle and second reagent bottle, be equipped with the spacing post of elasticity on the output shaft of motor, be equipped with on stirring sleeve pipe with the spacing spacing hole of post complex of elasticity, and be equipped with the limiting plate on the cell wall of third recess. The utility model discloses " have the function of even stirring reagent bottle, overall structure is simpler simultaneously for the installation of inside reagent bottle is more stable, improves the stability of transportation ".
Description
Technical field
The utility model is related to a kind of chemical instrument field, particularly a kind of kit for determining Serum CG content.
Background technology
Serum CG (Cholyglycine, CG) is one of mating type cholic acid that cholic acid is combined into glycine,
In liver cell, the enzymatic reaction of cholesterol process and its complexity is transformed into primary bile acid.Wherein there are cholic acid (CA) and goose to deoxygenate
Cholic acid（CD-CA）.When liver cell is impaired, liver cell intake CG abilities decline, and cause CG contents in blood to increase；Cholestegnosis
When, there is obstacle in hepatic excretion cholic acid, and sanguimotor CG contents of backflowing increase, and also increase blood CG contents；Oxyhepatitis,
CAH, primary carcinoma of liver, cirrhosis, chronic persistent hepatitis patient blood CG are obviously higher than normal person, and presentation property increases；
Cholelithiasis cause change of serum C G significantly to raise with Patients with Jaundice bile duct, gall-bladder excretory function obstacle；Cirrhosis, obstructive liver disease,
Intestines-hepatic circulatory disturbance change of serum C G level is higher than normal person.
The detection method of glycocholic acid mainly has following several：
1）Radioimmunology（radioimmunoassay,RIA）.This law has specificity good, sensitivity is high and instrument from
Dynamicization, it is easy to operate, the advantages of sample preparation is simple, but operating personnel's contact and to dispose radioactive substance be serious asking
Topic.
2）Chemiluminescence immunoassay（Chemiluminescence immunoassay, CLIA）.Chemiluminescence in principle is exempted from
Epidemic disease analysis bag contains two parts, i.e. chemiluminescence analysis system and immune response system.Chemiluminescence analysis system is utilizationization
The catalysis of luminescent substance Jing catalyst and the oxidation of oxidant are learned, the intermediate of an excitation state is formed, when in this excitation state
When mesosome returns to stable ground state, while launching photon (hM), using luminous signal measuring instrument quantum yield of luminscence is measured.Exempt from
Epidemic disease reaction system is that luminescent substance (exciting lower generation excitation state intermediate in reactant) is marked directly on into antigen (chemiluminescence
Immunoassay) or antibody (immunochemiluminometry) on, or enzyme effect is in luminous substrate.Chemiluminescence immunoassay is marking
Note method different and be divided into two kinds, i.e. chemiluminescent labeling immunoassay and chemiluminescence enzyme immunoassay.Chemiluminescence
Labelling immunoassay, also known as CLIA, is the immune analysis method with the direct labelled antigen of chemiluminescence agent or antibody.Chemiluminescence
EIA enzyme immunoassay (chemium inescen tenzyme immunoassay, CLEIA), belongs to EIA enzyme immunoassay, simply enzyme reaction
Substrate be luminous agent, it is identical that operating procedure exempts from analysis with enzyme：Bioactivator is marked (such as the antigen of enzyme mark with enzyme
Or antibody) immune response is carried out, the enzyme on immune response compound is remake for luminous substrate, is issued in signal reagent effect
Light, with luminous signal analyzer luminescence assays are carried out.At present conventional marker enzyme is horseradish peroxidase (HRP) and alkalescence
Phosphatase (ALP), they have respective luminous substrate.
3）Enzyme linked immunosorbent assay（enzyme linked immunosorbent assay , ELISA）.ELISA is
One kind of enzyme-linked immunosorbent assay, enzyme-linked immunosorbent assay is efficiently to urge the specificity of antigen-antibody reaction and enzyme to substrate
Change effect combine, according to after zymolyte develop the color, result of the test is judged with color change, can Jing enzyme mark analyzers determine
Amount analysis, susceptibility is up to ng levels.Antigen or antibody are adsorbed in surface of solid phase carriers, antigen-antibody reaction is carried in solid phase
Body surface face is carried out as ELISA.Antigen or antibody can be determined with indirect method, double antibody sandwich method or competition law.Current people on sale
CG ELISA kits, but application is less extensive.
4）Latex enhancing immune turbidimetry (Latex enhanced turbidimetric Immunoassay, LETIA).
The content of determinand is determined by the change of turbidity, this law can be using two kinds of competition law and non-competing method.The method measurement result
Stable, accuracy, reproducible, the range of linearity reaches 80mg/L, and to experimenter's non-hazardous, environment affinity is good, and be adapted to Hitachi,
The various automatic clinical chemistry analyzers such as Beckman, Abbott Laboratories, Mai Rui, Bayer, but cost is of a relatively high.
But current various methods more or less all be present, therefore how to design a cost relatively
The high kit of low, easy to operate, sensitivity is particularly important.
Utility model content
The purpose of this utility model is that a kind of sensitivity height, structure letter are provided to solve above-mentioned the deficiencies in the prior art
The kit of single, easy to operate, low cost, good stability and a kind of measure Serum CG content for stirring.
To achieve these goals, the kit of a kind of measure Serum CG content designed by the utility model, bag
Base, upper lid, the first reagent bottle and the second reagent bottle are included, the base and upper lid coordinate composition to accommodate the first reagent bottle and second
The cavity volume of reagent bottle, be provided with base for install the first reagent bottle the first groove, for the second reagent bottle is installed the
Two grooves, above the first reagent bottle and the second reagent bottle opening is equipped with, and lid is threaded with opening, in base
Be additionally provided with the 3rd groove and controller, the motor being connected with the controller in the 3rd groove, first reagent bottle and
Second reagent bottle is the double-deck Bottle structure that bottom is concave inward structure, and the internal layer of first reagent bottle and the second reagent bottle
Bottom is respectively connected with stirring sleeve of the outer layer rear enclosure for stretching out correspondence reagent bottle on the output shaft of motor, in the output shaft of motor
Elastic spacing post is provided with, the spacing hole coordinated with elastic spacing post, the stirring sleeve and motor are provided with stirring sleeve
Output shaft socket after can separate the internal layer bottom jack-up of correspondence reagent bottle and with the inwall of the first reagent bottle outer layer.
By said structure, can be by the way that the reagent bottle be put into into after various medicaments are put in corresponding reagent bottle
In three grooves, and corresponding stirring sleeve is placed on the output shaft of motor, is then turned on controller and opens motor so that motor
Operating, drives internal layer to rotate and causes the medicament in internal layer to carry out rotation so that each medicament is sufficiently stirred so that be last
Reagent is more uniform, and finally sensitivity also can be correspondingly improved when detection, and the entirety of the kit of said structure
Structure is simpler, cost is relatively low.
In order to improve stability, it is provided with above the first groove for the first locating rack of the reagent bottle of clamping first,
The top of the second groove is used for the second locating rack of the reagent bottle of clamping second.
For convenience dropping liquid test, is provided with liquid droping port on lid, and dropping liquid lid is hinged with liquid droping port.
A kind of kit of measure Serum CG content that the utility model is obtained, the work(with uniform stirring reagent bottle
Can, while overall structure is simpler, cost is lower, and the structure is improved and causes the installation of interior reagent bottle more stable, is carried
The stability of height transport.
Description of the drawings
Fig. 1 is that embodiment 1 provides a kind of sectional view of the kit of measure Serum CG content；
Fig. 2 is the partial enlarged drawing of part A in Fig. 1；
Fig. 3 is the partial enlarged drawing of part B in Fig. 1；
Fig. 4 is the partial enlarged drawing of C portion in Fig. 1；
Fig. 5 is that embodiment 2 provides a kind of sectional view of the kit of measure Serum CG content；
Fig. 6 is that embodiment 3 provides a kind of sectional view of the kit of measure Serum CG content.
In figure：Base 1, upper lid 2, the first reagent bottle 3, the second reagent bottle 4, the first groove 5, the second groove 6, the 3rd groove
7th, controller 8, motor 9, output shaft 9-1, stirring sleeve 10, elastic spacing post 11, spacing hole 12, dropping liquid lid 13, first are positioned
Frame 14, the second locating rack 15, opening 16, lid 2, liquid droping port 18.
Specific embodiment
The utility model is further illustrated with reference to the accompanying drawings and examples.
Embodiment 1：
As shown in Figure 1, Figure 2, Figure 3, Figure 4, the reagent of a kind of measure Serum CG content provided in the present embodiment
Box, including base 1, upper lid 2, the first reagent bottle 3 and the second reagent bottle 4, the base 1 and upper lid 2 coordinate composition to accommodate first
The cavity volume of the reagent bottle 4 of reagent bottle 3 and second, is provided with for installing the first groove 5 of the first reagent bottle 3, for pacifying in base 1
The second groove 6 of the second reagent bottle 4 is filled, opening 16 is equipped with the first reagent bottle 3 and the top of the second reagent bottle 4, in opening 16
Place is threaded with lid 17, is additionally provided with the 3rd groove 7 and controller 8 in the base 1, is connected with controller 8 located at the
Motor 9 in three grooves 7, the reagent bottle 4 of first reagent bottle 3 and second is the double-deck bottle knot that bottom is concave inward structure
Structure, and the internal layer bottom of the reagent bottle 4 of first reagent bottle 3 and second be respectively connected with stretch out correspondence reagent bottle outer layer rear enclosure in
Stirring sleeve 10 on the output shaft 9-1 of motor 9, on the output shaft 9-1 of motor 9 elastic spacing post 11 is provided with, in stirring set
Pipe 10 is provided with the spacing hole 12 coordinated with elastic spacing post 11, after the stirring sleeve 10 is socketed with the output shaft 9-1 of motor 9
Can separate by the internal layer bottom jack-up of correspondence reagent bottle and with the inwall of the outer layer of the first reagent bottle 3.
By said structure, can be by the way that the reagent bottle be put into into after various medicaments are put in corresponding reagent bottle
In three grooves, and corresponding stirring sleeve is placed on the output shaft of motor, is then turned on controller and opens motor so that motor
Operating, drives internal layer to rotate and causes the medicament in internal layer to carry out rotation so that each medicament is sufficiently stirred so that be last
Reagent is more uniform, and finally sensitivity also can be correspondingly improved when detection, and the entirety of the kit of said structure
Structure is simpler, cost is relatively low.
First reagent bottle 3 equipped with reagent 1 comprising the buffer solution of 10-100 mmol/L, turbid dose of the increasing of 0.1-1%w/v,
The preservative of 0.01-0.05%w/v；Second reagent bottle 4 is marked with AHS equipped with reagent 2 comprising 0.1-0.5%w/v
The sensitizing latex particle of glycocholic acid antibody, the stable material of 0.1-5%w/v, the buffer solution of 10-100 mmol/L, 0.01-0.05%
The anti-corrosion of w/v.
The preparation method of mentioned reagent 1 is adopted and added successively in the phosphate buffer of 50 mmol/L in the present embodiment
Final concentration of 0.1%（w/v）Polyethylene glycol, final concentration of 0.02%（w/v）NaN3, be sufficiently stirred for mixing, reagent Isosorbide-5-Nitrae is obtained
DEG C preserve.
The preparation method of mentioned reagent 2 is adopted：
1) NHS of 5 mg/mL of the EDC and 0.2 mL of 5 mg/mL of 0.2 mL is added in 1 mg present latex particulates；
2) room temperature（20-25 degree Celsius）The min of concussion reaction 30；
3) suspended particulate is rinsed with the phosphate buffer of 50 mmol/L, to remove unnecessary unreacted EDC and NHS,
Obtain latex suspension；
4) anti-human CG antibody is diluted to into 1 mg/mL with the phosphate buffer of 50 mmol/L；
5) antibody 10 the μ L, room temperature reaction 2h of dilution are added in latex suspension；
6) suspension is cleaned with the phosphate buffer of 50 mmol/L, it is unnecessary on latex particle to be not bonded to removing
Antibody；
7) 10000 rpm are centrifuged 20 min, supernatant discarded；
8) 10% is used（w/v）BSA is closed, room temperature reaction 1h；
9) 10000 rpm are centrifuged 20 min, supernatant discarded, the immune latex after being closed；
10) by closing after immune latex be resuspended in solution A, the solution A containing 10 mmol/L phosphate, 1%
（w/v）BSA and 0.02%（w/v）Sodium azide, concentration of the immune latex in solution A is 0.1%（w/v）.
Embodiment 2：
As shown in figure 5, a kind of general configuration of the kit of the measure Serum CG content provided in the present embodiment
It is same as Example 1, except for the difference that, in order to improve stability, it is provided with for the reagent bottle of clamping first in the top of the first groove 5
3 the first locating rack 14, in the top of the second groove 6 the second locating rack 15 of the second reagent bottle of clamping 4 is used for；By above-mentioned knot
The design of structure so that be prevented from body in transportation and rock, affects stability.
Embodiment 3：
As shown in fig. 6, a kind of general configuration of the kit of the measure Serum CG content provided in the present embodiment
Same as Example 1, except for the difference that, for convenience dropping liquid test, is provided with liquid droping port 18 on lid 17, cuts with scissors on liquid droping port 18
Dropping liquid lid 13 is connected to, when needing addition or extracting medicament, it is only necessary to test tube is extend in corresponding dropping liquid lid and is capable of achieving,
So be prevented from extract when bottle in medicament receive external environment pollution and impact effect.
Claims (3)
1. a kind of kit for determining Serum CG content, including base（1）, upper lid（2）, the first reagent bottle（3）With second
Reagent bottle（4）, the base（1）With upper lid（2）Coordinate to constitute and accommodate the first reagent bottle（3）With the second reagent bottle（4）Cavity volume,
In base（1）Inside it is provided with for installing the first reagent bottle（3）The first groove（5）, for installing the second reagent bottle（4）Second
Groove（6）, in the first reagent bottle（3）With the second reagent bottle（4）Top is equipped with opening（16）, in opening（16）Place's threaded connection
There is lid（17）, it is characterized in that：In base（1）Inside it is additionally provided with the 3rd groove（7）And controller（8）, in controller（8）Upper connection
Have located at the 3rd groove（7）Interior motor（9）, first reagent bottle（3）With the second reagent bottle（4）It is indent knot to be bottom
The double-deck Bottle structure of structure, and first reagent bottle（3）With the second reagent bottle（4）Internal layer bottom be respectively connected with stretch out correspondence
The outer layer rear enclosure of reagent bottle is in motor（9）Output shaft（9-1）On stirring sleeve（10）, in motor（9）Output shaft（9-1）
It is provided with elastic spacing post（11）, in stirring sleeve（10）It is provided with and elastic spacing post（11）The spacing hole of cooperation（12）, institute
State stirring sleeve（10）With motor（9）Output shaft（9-1）After socket can by correspondence reagent bottle internal layer bottom jack-up and with
First reagent bottle（3）The inwall of outer layer is separated.
2. a kind of kit for determining Serum CG content according to claim 1, is characterized in that：In the first groove
（5）Top be provided with for the reagent bottle of clamping first（3）The first locating rack（14）, in the second groove（6）Top be used for block
Connect the second reagent bottle（4）The second locating rack（15）.
3. a kind of kit for determining Serum CG content according to claim 1 and 2, is characterized in that：In lid
（17）It is provided with liquid droping port（18）, in liquid droping port（18）On be hinged with dropping liquid lid（13）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621116429.8U CN206082387U (en) | 2016-10-12 | 2016-10-12 | Kit of survey serum glycocholic acid content |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621116429.8U CN206082387U (en) | 2016-10-12 | 2016-10-12 | Kit of survey serum glycocholic acid content |
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| Publication Number | Publication Date |
|---|---|
| CN206082387U true CN206082387U (en) | 2017-04-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201621116429.8U Active CN206082387U (en) | 2016-10-12 | 2016-10-12 | Kit of survey serum glycocholic acid content |
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| CN (1) | CN206082387U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113041905A (en) * | 2021-03-17 | 2021-06-29 | 李亚军 | Stirring device and stirring method for printing ink |
-
2016
- 2016-10-12 CN CN201621116429.8U patent/CN206082387U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113041905A (en) * | 2021-03-17 | 2021-06-29 | 李亚军 | Stirring device and stirring method for printing ink |
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