CN206057339U - Immuno-chromatographic test paper strip and portable detector - Google Patents
Immuno-chromatographic test paper strip and portable detector Download PDFInfo
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- CN206057339U CN206057339U CN201620452415.7U CN201620452415U CN206057339U CN 206057339 U CN206057339 U CN 206057339U CN 201620452415 U CN201620452415 U CN 201620452415U CN 206057339 U CN206057339 U CN 206057339U
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Abstract
This utility model provides a kind of immuno-chromatographic test paper strip for detection by quantitative Aflatoxins M1.The immuno-chromatographic test paper strip includes analyte binding regions and detection line, and the analyte binding regions are coated with quantum dot-labeled Aflatoxins M1 monoclonal antibody, and the detection line is coated with Aflatoxins M1 antigen/hapten-carrier protein conjugate.The emission wavelength of the quantum dot-labeled Aflatoxins M1 monoclonal antibody is 520 ~ 650 nm, and wherein each quantum dot has at most been coupled 2 Aflatoxins M1 monoclonal antibodies.This utility model additionally provides a portable detector, coordinates the immuno-chromatographic test paper strip to carry out detection by quantitative to the Aflatoxins M1 in sample.The portable detector includes light source module and display module, and the light source module is used for the quantum dot light emitting for exciting the immuno-chromatographic test paper strip, the concentration of Aflatoxins M1 in the display module quantitative display sample.
Description
Technical field
This utility model is related to field of food detection, and in particular to one kind is based on preferred material, glimmering using quantum dot
The immuno-chromatographic test paper strip and portable detector of light immunochromatography Quantitative detection Aflatoxins M1.
Background technology
Aflatoxin is by some mycetes（Such as Aspergillus flavus and aspergillus parasiticus）One group of chemical constitution that metabolism is produced is similar,
Intoxicating group identical compound, Jing is often in storing mode improperly Semen arachidis hypogaeae, Semen Maydiss, Oryza sativa L., Semen Tritici aestivi, Sorghum vulgare Pers., sunflower seed, heavily fortified point
Really, find in the staple commodities such as cotton seeds.It is at present separated to identify more than 20 and plant aflatoxin, wherein, mainly there is Huang Qu
Syphilis element B1, B2, G1, G2 and by B1 and B2 in vivo through hydroxylation metabolite M1, M2 for being derivatized to etc..Aflatoxin
B1 and M1 are the mutagenic toxic compounds of carcinogenic teratogenesis, the position such as main harm liver, kidney, while having immunity poison
Property, immunocompetence is reduced, human health is worked the mischief.Two kinds are generally included by the food that Aspergillus flavus pollute, the first is yellow
Aspertoxin（Predominantly B1）The plant food of pollution, which two is by aflatoxin via feedstuff（Predominantly M1）Pollution
Breast or milk product（Such as cheese, milk powder etc.）.In order to safeguard human health, multiple countries and regions are all to aflatoxin in food
Content done strict limitation requirement.
Main source of the milk product as infant food, is raised year by year in the consumption figure of China, its quality security problem
Often cause the extensive concern of society.China exists《National food safety standard --- mycotoxin limitation in food》（GB
2761-2011）Middle regulation, in milk and milk productses and food for special foods（Such as all kinds of infant formulas）Middle aflatoxin
The content of M1 must not exceed 0.5 μ g/kg.The relevant criterion of European Union more strictly specifies aflatoxin in milk and milk productses
The content of M1 must not exceed 0.05 μ g/kg, and in infant formula, the content of Aflatoxins M1 must not exceed 0.025 μ g/kg.
The detection method of Aflatoxins M1 mainly have chromatography, immuno-chemical method, electrochemical method, chemiluminescence and various methods it
Between combination.However, there is sample pre-treatments complexity, instrument and equipment costliness, detection time length etc. in the instrument analytical method of routine
Problem, it is impossible to the requirement that satisfaction is used for quickly detecting to sample at the scene.Chinese patent 201410079913.7 and Chinese patent
201520275946.9 being based on immunochromatography (immunochromatography) technology, it is utilized respectively and is embedded with fluorescent dye
Fluorescent microsphere and colloid gold label Aflatoxins M1 monoclonal antibody, prepare detection Aflatoxins M1 immunity-chromatography test
Paper slip.Compared with traditional detection method, both immuno-chromatographic test paper strips have the features such as simple to operate, detection time is shorter.
But, these methods yet suffer from certain deficiency.The former is using the fluorescent microsphere based on traditional organic fluorescent dye as glimmering
Light probe, traditional organic fluorescent dye luminescent lifetime are shorter, and stability is not good enough, photobleaching phenomenon easily occur, and using in addition should
Test strips need to heat sample during being detected, operation is relatively complicated, increased work required when detecting a large amount of samples
Measure；As absorbing, probe, colloid gold particle uniform particle diameter are poor using colloid gold particle for the latter, and immune marker is unstable,
Clever density is relatively low.Importantly, prior art can only carry out qualitative detection, it is impossible to quick in laboratory and production scene
Detection breast and milk product etc. in Aflatoxins M1 content, it is difficult to directly judge whether Related product has reached related food
Product safety criterion.
Be otherwise known as quantum dot (quantum dots, QDs) semiconductor nano, generally by II-VI group and III-V
Race is elementary composition, with size adjustable, exciting light spectrum width, emission spectrum is narrow, stoke shift is larger the features such as.Quantum dot mole
Specific absorbance and fluorescence quantum yield are higher, can produce stronger fluorescence signal, improve the sensitivity of detection.Meanwhile, amount
Son point photochemical stability is good, fast light bleachability is good, improves the stability of testing result.In sum, quantum dot is one
Preferable fluorescence probe material is planted, before the detection field based on immunochromatography technique has unique advantage and is widely applied
Scape.At this stage, the quantum dot as fluorescent probe mainly has monokaryon quantum dot (CdSe, CdTe, CdS) and core-shell type quantum
Point (CdSe/ZnS, CdSe/ZnSe), can be based between Organic substance and electrodeless metallic compound or organo-metallic compound
Pintsch process reacts and prepares in non-polar organic solvent.Synthesize in organic system the quantum dot for obtaining have it is almost ideal
Crystal structure, good monodispersity, stronger light stability and higher fluorescent yield, but used in conventional synthesis process
Tributylphosphine, hypertoxic, inflammable, the unstable organic phosphine compound such as tri octyl phosphine, explosive, expensive, and to operation
Environment has higher requirement, limits the large-scale production and application of quantum dot product.Additionally, preparing quantum dot fluorescence probe
During, quantum dot and the coupled product of corresponding antibody molecule have that homogeneity and dispersibility are poor, be easy to occur to reunite,
The problems such as luminous efficiency is relatively low, emission spectrum half-peak breadth is wide, have impact on the detection performance of Related product.
Utility model content
This utility model overcomes deficiency of the prior art, using immunochromatography technique and quanta point material, there is provided
A kind of immuno-chromatographic test paper strip and portable detector of Quantitative detection Aflatoxins M1.
This utility model provides a kind of immuno-chromatographic test paper strip for detection by quantitative Aflatoxins M1, including sample
Land and detection line.The analyte binding regions are coated with quantum dot-labeled Aflatoxins M1 monoclonal antibody, the detection line
It is coated with Aflatoxins M1 antigen/hapten-carrier protein conjugate.The quantum dot-labeled Aflatoxins M1 monoclonal
Each quantum dot in antibody is at most coupled 2 Aflatoxins M1 monoclonal antibodies, the quantum dot-labeled Aspergillus flavus poison
The emission wavelength of plain M1 monoclonal antibodies is 520 ~ 650 nm.
Preferably, the detection line is coated with Aflatoxins M1 hapten-bovine serum albumin conjugate
Further, the immuno-chromatographic test paper strip also includes nature controlling line, and the nature controlling line is coated with sheep anti-mouse antibody.
Preferably, the quantum dot used in this utility model be water-soluble quantum dot, the diameter of the water-soluble quantum dot
For 30 ~ 60 nm.
Further, the water-soluble quantum dot elder generation Jing oil phases used in this utility model synthesize Jing water phase transfer system again
.The oil phase synthesis step prepares oil-soluble quantum dot using without phosphine method.Amphipathy macromolecule is adopted in the water phase transfer step
Coat the oil-soluble quantum dot.Interaction force between the amphipathy macromolecule and the oil-soluble quantum dot is made for non-covalent bond
With.
Preferably, a diameter of 10 ~ 30 nm of the oil-soluble quantum dot, the oil-soluble quantum dot be CdS, CdSe, CdTe,
ZnS、ZnSe、CdSe/ZnS、CdSe/CdS、CdSe/CdS/ZnS、ZnSe/ZnS、ZnSe/CdSe/ZnS、CuInS2/ZnS、
CuInZnxS2+x/ZnS、CdxZn1-xOne or more in Se/ZnS, CdS/ZnSe, ZnSe/CdSe/ZnS, CdTe/CdSe
Combination.
Preferably, the amphipathy macromolecule includes polymaleic anhydride hexadecanol ester.
In a preferred embodiment, the Monitoring lower-cut of the immuno-chromatographic test paper strip be 0.05 ng/mL, detection by quantitative model
Enclose for 0.05 ~ 1.0 ng/mL.
Preferably, the immuno-chromatographic test paper strip also includes Quick Response Code, and the Quick Response Code records the information of immuno-chromatographic test paper strip
With detection project data.
This utility model additionally provides a portable detector, coordinates the immuno-chromatographic test paper strip detection by quantitative Aspergillus flavus
Toxin M1.The portable detector includes light source module and display module.The wavelength of the light that the light source module is produced is 360 ~ 485
Nm, for exciting the quantum dot light emitting of the immuno-chromatographic test paper strip；Aspergillus flavus poison in the display module quantitative display sample
The concentration of plain M1.
Further, the portable detector also includes reading code module, and the reading code module can read Quick Response Code.The two dimension
Code records the information and detection project data of immuno-chromatographic test paper strip.
This utility model is by the quantum dot that adopted to immuno-chromatographic test paper strip and quantum dot-labeled aflatoxin
The structure and performance of M1 monoclonal antibodies is strictly controlled, and the detection card can be carried out to Aflatoxins M1 at normal temperatures soon
Fast, sensitive, accurate detection by quantitative, has linear relationship in larger concentration ranges, and the stability of testing result is high, quantitatively
The deviation of testing result is less than 15%, and detection sensitivity can reach 0.05 ng/mL, meet domestic and international relevant food safety post
The accurate detection demand to Aflatoxins M1 content in sample.
Description of the drawings
Fig. 1 is the structural representation of immuno-chromatographic test paper strip in first embodiment.
Fig. 2 is to synthesize the schematic diagram of quantum dot-labeled Aflatoxins M1 monoclonal antibody in first embodiment.
Fig. 3 is the structural representation of immuno-chromatographic test paper strip in another embodiment.
Fig. 4 is the standard curve that immuno-chromatographic test paper strip detects Aflatoxins M1 used in a specific embodiment.
It should be pointed out that what above-mentioned accompanying drawing was not drawn to scale, and in accompanying drawing analog structure or function component
Generally represented by identical label for purposes of illustration.It is also important to note that accompanying drawing is for only for ease of preferred embodiment
Description.Accompanying drawing does not show the every aspect of described embodiment, does not limit scope of the present utility model yet.
Main element symbol description
| Immuno-chromatographic test paper strip | 100,200 |
| Sample application zone | 10 |
| Well | 12 |
| Sample | 14 |
| Analyte binding regions | 20 |
| Quantum dot-labeled Aflatoxins M1 monoclonal antibody | 22 |
| Aflatoxins M1 monoclonal antibody | 24 |
| Water-soluble quantum dot | 26 |
| Oil-soluble quantum dot | 27 |
| Amphipathy macromolecule | 28 |
| Chromatographic film | 32 |
| Detection line | 34 |
| Nature controlling line | 36 |
| Suction zones | 40 |
| End liner | 50 |
| Quick Response Code | 210 |
| Shell | 220 |
| Immunochromatography detection zone | 230 |
Following specific embodiment will further illustrate this utility model with reference to above-mentioned accompanying drawing.
Specific embodiment
Fig. 1 is referred to, first embodiment of the present utility model provides a kind of for detection by quantitative Aflatoxins M1
Immuno-chromatographic test paper strip 100.The immuno-chromatographic test paper strip 100 includes end liner 50, sample application zone 10, analyte binding regions 20, chromatography
Film 32, suction zones 40 are pasted onto on end liner 50 in order.The sample application zone 10 has well 12.The top of the sample application zone 10 with
The top alignment of the end liner 50, the end of the suction zones 40 are alignd with the end of the end liner 50, the end of the analyte binding regions 20
It is connected with the top of the chromatographic film 32, the end of the chromatographic film 32 is connected with the suction zones 40.There is in the chromatographic film 32 band
The detection line 34 and nature controlling line 36 of shape, perpendicular to the longer both sides of the immuno-chromatographic test paper strip 100.The detection line 34 is coated with Huang
Aspertoxin M1 antigens/hapten-carrier protein conjugate, positioned at the one end near analyte binding regions 20；The nature controlling line 36
In the one end away from analyte binding regions 20.
In one embodiment, the analyte binding regions 20 be glass fibre membrane, the chromatographic film 32 be NC Nitroncellulose, the detection
Line 34 is coated with Aflatoxins M1 hapten-bovine serum albumin conjugate, and the nature controlling line 36 is coated with sheep anti-mouse antibody, should
Suction zones 40 are absorbent paper, and the end liner 50 is PVC base plates.
To realize the detection by quantitative to Aflatoxins M1, the analyte binding regions 20 are coated with quantum dot-labeled Aspergillus flavus
Toxin M1 monoclonal antibodies 22, wherein each quantum dot surface have at most been coupled 2 Aflatoxins M1 monoclonal antibodies, transmitting
Wavelength is 520 ~ 630 nm.As shown in Fig. 2 the quantum dot-labeled Aflatoxins M1 monoclonal antibody 22 is by being coated with two
The high molecular water-soluble quantum dot 26 of parent's property is with Aflatoxins M1 monoclonal antibody 24 by being coupled (bioconjugation)
Reaction is obtained.A diameter of 30 ~ 60 nm of the water-soluble quantum dot 26.Specifically, the preparation of the water-soluble quantum dot 26 includes
Oil phase synthesizes and two steps of water phase transfer, i.e., by the synthetically prepared oil-soluble quantum dot 27 of oil phase and by water phase transfer by two
Parent's property macromolecule 28 is coated on 27 surface of oil-soluble quantum dot.
Wherein, the oil-soluble quantum dot 27 selected from CdS, CdSe, CdTe, ZnS, ZnSe, CdSe/ZnS, CdSe/CdS,
CdSe/CdS/ZnS、ZnSe/ZnS、ZnSe/CdSe/ZnS、CuInS2/ZnS、CuInZnxS2+x/ZnS、CdxZn1-xSe/ZnS、
The combination of one or more in CdS/ZnSe, ZnSe/CdSe/ZnS, CdTe/CdSe.The oil-soluble quantum dot 27 is to adopt nothing
Quantum dot with nucleocapsid structure prepared by phosphine method (phosphine-free synthesis), its a diameter of 10 ~ 30 nm,
Emission wavelength is 520 ~ 650 nm.The quantum dot of required particle diameter and launch wavelength is obtained by controlling the response time.
Wherein, the hydrophobic side of the amphipathy macromolecule 28 is by non-covalent bonds such as hydrophobic forces, Van der Waals force or hydrogen bonds
Effect is interacted with the hydrophobic group on 27 surface of oil-soluble quantum dot, is coated on the surface of the oil-soluble quantum dot 27, is obtained
To the water-soluble quantum dot 26.By the bar for further controlling the molecular weight and water phase transfer reaction of the amphipathy macromolecule 28
Part, the particle diameter of the prepared water-soluble quantum dot 26 is 30 ~ 60nm.In one embodiment, the amphipathy macromolecule 28 is poly-
Maleic acid hexadecanol ester.
Wherein, the water-wet side of the amphipathy macromolecule 28 is had and can be formed altogether with the Aflatoxins M1 monoclonal antibody 24
The group that valence link or intermolecular specificity interact, prepares the quantum dot-labeled Aflatoxins M1 list by coupling reaction
Clonal antibody 22.The coupling reaction can both be that the covalent bond realized using chemical cross-linking agent is coupled, it is also possible to using intermolecular
Specificity interacts（Or molecular recognition, molecular recognition）The non-covalent strong coupling realized.In this practicality
In a new embodiment, the coupling reaction adopts 1- (3- dimethylamino-propyls) -3- ethyl carbodiimides (EDC) and N- hydroxyls
Used as cross-linking agent, its general step is base thiosuccimide (Sulfo-NHS)：By the water-soluble quantum dot 26 and EDC and
NHS after mix homogeneously, is added thereto to a certain amount of Aflatoxins M1 monoclonal antibody in buffer, reacts certain hour
Afterwards, 1% bovine serum albumin or the closing of lact albumin hydrolysate solution are added, purification is carried out to product by being centrifuged repeatedly, washing.
Make this quantum dot-labeled by controlling the mol ratio between each reactant, addition/hybrid mode, reaction temperature, response time etc.
Aflatoxins M1 monoclonal antibody 22 in each quantum dot at most be coupled have 2 antibody.Using agarose gel electricity
Swimming is characterized to prepared quantum dot-labeled Aflatoxins M1 monoclonal antibody 22, observes which in agarose gel
Displacement and migration rate, obtain the quantity of the Aflatoxins M1 monoclonal antibody 24 that the water-soluble quantum dot 26 is coupled.
The absorbance value of the quantum dot-labeled Aflatoxins M1 monoclonal antibody 22, fluorescent value and the fluorescence response rate
Can be detected by continuous wavelength multi-function microplate reader.Additionally, the method is realized to sample using the light absorption value of sample solution
The quantitative control of the concentration of quantum dot in product.In one embodiment, 10 μ L samples are added into 240 μ L buffer dilution 25
Times, after mixing, sample in the sample solution addition ELISA Plate hole after 100 μ L dilutions, carry out multiple holes test.Existed using end-point method
The absorption value of the sample solution at 400nm wavelength after simple scan detection dilution.Multiple holes data result is averaged, then with
Absorption value carries out constant volume to sample for standard for 2.0.Afterwards, the buffer of 399 μ L is added to dilute 400 times 1 μ L samples.It is mixed
After even, take in the sample solution addition ELISA Plate hole after 150 μ L dilutions, carry out multiple holes test.Under the excitation wavelength of 365 nm
Fluorescent value in the range of 610 nm-650 nm of continuous scanning, averages to multiple holes data result, restores to former times（With 400
It is multiplied by the meansigma methodss）The fluorescent value of the quantum dot-labeled Aflatoxins M1 monoclonal antibody 22 after being coupled.To be coupled
The fluorescent value of product obtains fluorescence divided by the fluorescent value using water-soluble quantum dot 26 before the coupling obtained by same detection method
The response rate.
In a preferred embodiment, the quantum dot-labeled aflatoxin to the different batches with identical absorbance value
The fluorescent value and the fluorescence response rate of M1 monoclonal antibodies 22 is detected.Under the testing result that represents show, this utility model
The embodiment of offer can drop to the fluorescence losses of quantum dot after coupling reaction within 30%.
| Coupled product is numbered | Fluorescent value after coupling | The fluorescence response rate | Absorbance value |
| C6816032401 | 6.9422E+10 | 75.60% | 2.0 |
| C6816032404 | 6.5011E+10 | 70.80% | 2.0 |
| P6816032413 | 6.4859E+10 | 70.63% | 2.0 |
| M6816032416 | 6.4481E+10 | 70.22% | 2.0 |
| M6816032419 | 6.4877E+10 | 70.65% | 2.0 |
| C7016041214 | 1.1265E+11 | 77.43% | 2.0 |
| C7016041502 | 1.164E+11 | 73.04% | 2.0 |
| P7016042003 | 1.1725E+11 | 79.19% | 2.0 |
| M7016041202 | 1.1287E+11 | 79.76% | 2.0 |
| F7016042105 | 1.0738E+11 | 72.45% | 2.0 |
| M7016042110 | 1.1369E+11 | 76.12% | 2.0 |
Further, immuno-chromatographic test paper strip provided by the utility model can also have Quick Response Code.Refer to Fig. 3,
In another embodiment, immuno-chromatographic test paper strip 200 includes Quick Response Code 210, immunochromatography detection zone 230 and shell 220.Its
In, the Quick Response Code 210 is arranged at the surface of shell 220, and the immunochromatography detection zone 230 is arranged at the interior of the shell 220
Portion.The Quick Response Code 210 records detection project data, including the name information of the detection card, detection project, product batch number, matter
The minimum effective brightness in control area, calibration curve equation, shelf-life etc..Importantly, the detection project described in Quick Response Code 210
Data include one or more detection pattern corresponding to the detection project, and the detection needed for every kind of detection pattern is when waiting
Between.Such as above immunoassay test strips 110 are essentially identical for the structure of the immunochromatography detection zone 230, and here is omitted.Will
The immunochromatography detection zone 230 loads in the shell 220 with Quick Response Code 210.
Second embodiment of the present utility model provides a portable detector, coordinates foregoing immunity-chromatography test
Paper slip realizes the detection by quantitative to Aflatoxins M1.The portable detector includes light source module, display module and power supply mould
Block.Wherein, the light source module can launch wavelength for 360-485 nm ultraviolet light or blue light, the display module directly can show
Show the concentration of Aflatoxins M1 in sample, the supply module is the low-voltage lithium battery installed in 220V power supplys or machine.
In sample shown by the display module, the concentration of Aflatoxins M1 is calculated by formula 1：
Y = (A-D)/{1+(X/C)B+ D formula 1
Wherein, A, B, C, D are four fitting parameters, and X is the concentration of Aflatoxins M1, when Y is to be excited by same light source
The ratio of the luminous intensity that detection zone (T) and quality control region (C) detection are obtained（T/C ratios）.
To coordinate the use of above-mentioned immuno-chromatographic test paper strip 200, in another embodiment, the portable detector enters one
Step includes reading code module.The reading code module can read the detection described in Quick Response Code 210 in the immuno-chromatographic test paper strip 200
Project data.When the immuno-chromatographic test paper strip 200 added with sample being inserted the portable detector being detected, this takes formula inspection
Survey instrument and read the detection project data that the Quick Response Code 210 is recorded first, judge whether used detection card is expired, detection card institute
Whether corresponding production batch is qualified, the concentration range of the type of tested subject matter and the tested subject matter that accurately can be detected
（The range of linearity）Etc. information.If the detection project data for reading are satisfied by corresponding conditionses, the portable detector is according to the two dimension
The detection project data that code 210 is recorded determine the detection waiting time, at the corresponding moment with the light source activation immuno-chromatographic test paper strip
Quantum dot light emitting in 200, processes and analyzes the optical signal that the immuno-chromatographic test paper strip 200 is produced, and shows testing result, i.e., yellow
The concentration of aspertoxin M1.
Using the method for Aflatoxins M1 in 100 detection by quantitative sample of immuno-chromatographic test paper strip, comprise the following steps：
One portable detector is provided., with described in second embodiment, here is omitted for the portable detector.
Prepare sample 14.In a specific embodiment, the sample 14 is milk product, need to be selected according to the feature of the milk product
Select different sample handling characteristics.If the sample 14 is liquid milk product, for liquid milk product of the fatty degree more than 5%, by which
It is centrifuged 5 minutes with the rotating speed of 6 000 rpm at 4 DEG C, after removing upper-layer fat layer, removes layer liquid to be checked；For fatty degree
Liquid milk product less than 5%, can be directly used for detection.If the sample 14 is solid-state milk product, such as milk powder etc., by solid-state milk
Product presses 1:8 ratio is dissolved in distilled water and is configured to liquid milk, i.e., the distilled water of 8 g is added in the milk powder of 1g, joins after mixing
Sample is processed according to the correlation technique of liquid milk product.
In a specific embodiment, before detection, the immuno-chromatographic test paper strip 100 is placed in into room temperature environment first.Treat its balance
To room temperature, check whether the outer package of the immuno-chromatographic test paper strip 100 is intact, confirm to open the package after which is intact to take out and be somebody's turn to do
Immuno-chromatographic test paper strip 100.Meanwhile, the portable detector, pre- thermal light source are opened in advance.The sample 14 is located as stated above
After reason, the sample-adding that the sample 14 after 80 μ L are processed lentamente vertically instills the immuno-chromatographic test paper strip 100 is taken with pipettor
Hole 12.Then, by the immuno-chromatographic test paper strip 100 insert the portable detector in the sample 14 Aflatoxins M1 it is dense
Degree carries out detection by quantitative.
In another embodiment, using the immuno-chromatographic test paper strip 200 with above-mentioned Quick Response Code 210, coordinate with reading
The content of Aflatoxins M1 in the portable detector detection by quantitative sample of code module.In one embodiment, by after process
After sample instills the well of the immuno-chromatographic test paper strip 200, the immuno-chromatographic test paper strip 200 is inserted into the portable inspection immediately
Survey instrument and select " standard detection " pattern；After the portable detector reads the detection project data that the Quick Response Code 210 is recorded, enter
Enter countdown state, the dense of Aflatoxins M1 in sample is detected and be given after the waiting time for pre-setting
Degree.In another embodiment, after the sample after process to be instilled the well of the immuno-chromatographic test paper strip 200, by the immune layer
After analysis test strips 200 are stored at room temperature 15 minutes, then the immuno-chromatographic test paper strip 200 are inserted into the portable detector and is selected
" quick detection " pattern；The portable detector is directly proceeded by after reading the detection project data that the Quick Response Code 210 is recorded
Detect and provide the concentration of Aflatoxins M1 in sample.
In one embodiment, using the immuno-chromatographic test paper strip 100 and portable inspectiont disclosed by this utility model
Instrument, according to above-mentioned sample treatment and detection method, detects the standard sample of one group of variable concentrations.Fig. 4 is referred to, x-axis is yellow bent
The concentration of syphilis element M1, y-axis are detection line 34 on immuno-chromatographic test paper strip 100 during detection（T）On fluorescence intensity and nature controlling line
36（C）On fluorescence intensity ratio（T/C values）.Detection by quantitative is obtained by tetra- parameter curve models fittings of Logistic yellow bent
The calibration curve equation of syphilis element M1：y = (7.02+0.34) / {1+(x/0.14)0.84- 0.34 (i.e. be fitted after formula
A=7.02 in 1, B=0.84, C=0.14, D=0.34).In the concentration range of 0.05 ng/mL ~ 1 ng/mL, the fitting is bent
The correlation coefficient r of line square（r2）For 0.993, illustrate the immuno-chromatographic test paper strip disclosed by this utility model in the concentration model
There is in enclosing extraordinary linear relationship, detection sensitivity can reach 0.05 ng/mL, disclosure satisfy that domestic and international all correlations
Detection of the food security standard to Aflatoxins M1 content in sample is required.
This utility model realize production scene, laboratory or testing agency rapidly and accurately detection by quantitative breast, breast system
The content of Aflatoxins M1 in product and related diet and foodstuffs.This utility model is by controlling quantum dot-labeled aflatoxin
The property of the coupled product of M1 monoclonal antibodies, improve the dispersibility of quantum dot-antibody coupling matter, it is to avoid quantum dot-anti-
The generation that body conjugate is reunited, so as to improve the stability and susceptiveness of immunity test strip product.
Additionally, this utility model simplifies the preparation technology of the immuno-chromatographic test paper strip detection card based on quantum dot probe,
Higher, to the reaction condition requirement harsh organic phosphine compound of toxicity is avoided during synthesis quantum dot particularly
Use, alleviate the pollution to environment and the potential hazard to related experiment personnel.This utility model is also adopted to detection card
Quantum dot and the structure and performance of quantum dot-labeled Aflatoxins M1 monoclonal antibody carried out strict control, obtain
With high luminous intensity, high quantum production rate, stable dispersion quantum dot probe, so as to improve detection Aflatoxins M1
The repeatable and credibility of sensitivity, accuracy, the scope of application and testing result.
This utility model can read the detection mesh number described in the Quick Response Code on detection card by portable detector
A series of anticipations and Data Enter are carried out according to detection card and sample, the workload of testing staff is alleviated, is simplified inspection
Survey process, improves detection speed, it is adaptable to the Quantitative detection of batch samples.
The utility model discloses a kind of immuno-chromatographic test paper strip of quick, sensitive, detection by quantitative Aflatoxins M1 and
Portable detector.It should be appreciated that the embodiment is not limited to the concrete form for having disclosed, on the contrary, this practicality
It is new by all changes covered in this utility model concept, substitute and shift gears.
Claims (7)
1. a kind of immuno-chromatographic test paper strip for detection by quantitative Aflatoxins M1, including analyte binding regions and detection line, institute
State analyte binding regions and be coated with quantum dot-labeled Aflatoxins M1 monoclonal antibody, the detection line is coated with Aspergillus flavus poison
Plain M1 antigens/hapten-carrier protein conjugate；Each in the quantum dot-labeled Aflatoxins M1 monoclonal antibody
Quantum dot is at most coupled 2 Aflatoxins M1 monoclonal antibodies, the quantum dot-labeled Aflatoxins M1 monoclonal
The emission wavelength of antibody is 520～650nm.
2. immuno-chromatographic test paper strip as claimed in claim 1, it is characterised in that the immuno-chromatographic test paper strip also includes Quality Control
Line, the detection line are coated with Aflatoxins M1 hapten-bovine serum albumin conjugate, and the nature controlling line is coated with goat-anti
Murine antibody.
3. immuno-chromatographic test paper strip as claimed in claim 1, it is characterised in that the quantum dot is water-soluble quantum dot, institute
State a diameter of 30～60nm of water-soluble quantum dot.
4. immuno-chromatographic test paper strip as claimed in claim 1, it is characterised in that the Monitoring lower-cut of the immuno-chromatographic test paper strip
For 0.05ng/mL, detection by quantitative scope is 0.05～1.0ng/mL.
5. immuno-chromatographic test paper strip as claimed in claim 1, it is characterised in that the immuno-chromatographic test paper strip includes two dimension
Code, the Quick Response Code record the information and detection project data of the immuno-chromatographic test paper strip.
6. a kind of portable detector, coordinates immuno-chromatographic test paper strip detection by quantitative aflatoxin as claimed in claim 1
M1, including light source module and display module；The wavelength of the light that the light source module is produced is 360～485nm, described for exciting
The quantum dot light emitting of immuno-chromatographic test paper strip；The concentration of Aflatoxins M1 in the display module quantitative display sample.
7. portable detector as claimed in claim 6, it is characterised in that the portable detector includes reading code module,
The reading code module can read Quick Response Code, and the Quick Response Code records the information and detection mesh number of the immuno-chromatographic test paper strip
According to.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108717054A (en) * | 2018-04-26 | 2018-10-30 | 河南省农业科学院农业质量标准与检测技术研究所 | A kind of quantum dot-labeled antibody probe test strips and its preparation method and application |
| CN109490538A (en) * | 2018-12-27 | 2019-03-19 | 公安部第研究所 | A kind of fluorescence immunoassay trace hair drugs four-in-one detection kit |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108717054A (en) * | 2018-04-26 | 2018-10-30 | 河南省农业科学院农业质量标准与检测技术研究所 | A kind of quantum dot-labeled antibody probe test strips and its preparation method and application |
| CN109490538A (en) * | 2018-12-27 | 2019-03-19 | 公安部第研究所 | A kind of fluorescence immunoassay trace hair drugs four-in-one detection kit |
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