CN206052013U - A kind of centrifuge tube with cell filter - Google Patents

A kind of centrifuge tube with cell filter Download PDF

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Publication number
CN206052013U
CN206052013U CN201620650132.3U CN201620650132U CN206052013U CN 206052013 U CN206052013 U CN 206052013U CN 201620650132 U CN201620650132 U CN 201620650132U CN 206052013 U CN206052013 U CN 206052013U
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China
Prior art keywords
drive rod
cartridge filter
filter
centrifuge tube
cell
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CN201620650132.3U
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Chinese (zh)
Inventor
刘召宏
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Nanjing Jianbang Jinyuan Medical Treatment Co Ltd
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Nanjing Jianbang Jinyuan Medical Treatment Co Ltd
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Priority to CN201620650132.3U priority Critical patent/CN206052013U/en
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Abstract

The utility model discloses a kind of centrifuge tube with cell filter, belong to a kind of cell culture centrifuge tube, aim to provide a kind of centrifuge tube with cell filter, delay with the cell solution reduced on filter membrane, the advantage for avoiding cell solution from wasting, a kind of centrifuge tube with cell filter of its drip irrigation device, including the body of upper end open, the upper end of the body is equipped with cartridge filter, the cartridge filter upper end is provided with the baffle plate for being fixed on cartridge filter on body, the cartridge filter is provided with angular cut, the cartridge filter lower end forms a point with angular cut, the cartridge filter is provided with the first filter membrane for covering angular cut in the position of angular cut.

Description

A kind of centrifuge tube with cell filter
Technical field
This utility model is related to a kind of cell culture centrifuge tube, more particularly to a kind of centrifuge tube with cell filter.
Background technology
Centrifuge tube with cell filter causes cell solution that early stage filtration is carried out when centrifuge tube is poured into, and volume is larger Cell is filtered out coming so that the later stage rotates the rotation time required for separating and reduces, so as to reduce the loss of the energy, energy-conservation Environmental protection.
The Chinese patent of Publication No. CN203569111U discloses a kind of centrifuge tube with cell filter, including pipe The lid under body, lid, apocarpy is threaded connection the upper end of the body, and which also includes cell filter, institute State cell filter and specifically include framework, filter membrane, the framework includes top anchor ring, bottom anchor ring, middle brace rod, institute State, the filter membrane includes bottom filter membrane, exterior annular filter Film, the lower surface of the excircle part of the bottom filter membrane are supported on the inner top side face in the lower annular face.But when should be from When the bottom filter membrane of the cell filter in heart pipe is blocked by impurity, some cell solutions can be flowed out from exterior annular filter membrane, and The cell solution in later stage can not flow on the filter membrane of bottom as bottom filter membrane is horizontally disposed, and then be trapped in bottom filter membrane On, cause the waste of cell solution.
Utility model content
The purpose of this utility model is to provide a kind of centrifuge tube with cell filter, with the cell reduced on filter membrane The delay of solution, it is to avoid the advantage that cell solution is wasted.
Above-mentioned technical purpose of the present utility model technical scheme is that:A kind of band cell filter Centrifuge tube, including the body of upper end open, the upper end of the body be arranged with cartridge filter, the cartridge filter upper end be provided with for Baffle plate cartridge filter being fixed on body, the cartridge filter are provided with angular cut, and the cartridge filter lower end is formed with angular cut One point, the cartridge filter are provided with the first filter membrane for covering angular cut in the position of angular cut.
Tubular body is entered from body open by adopting above-mentioned technical proposal, cartridge filter, by the pipe at body open Support of the wall to baffle plate causes cartridge filter to be fixed in body, and cell solution is entered in cartridge filter, will be thin by the first filter membrane Contaminant filter in cell lysis liquid falls so that the Cell sap of needs is collected in body by the first filter membrane, is filtering Angular cut is opened up on cylinder, now the first filter membrane is covered on angular cut, the angle of inclination of the first filter membrane is increased, and then Filter area is increased, when cell solution is flowed in cartridge filter, cartridge filter lower end forms a point with angular cut, therefore Cell solution will not be piled up in cartridge filter lower end, and with progressing into for cell solution, cell solution is filtered from inclined first Gradually flow out on film, as the first filter membrane is obliquely installed, cell solution will not be detained on the first filter membrane, due to cartridge filter Lower end forms a point with angular cut, it is to avoid cell solution is trapped in cartridge filter lower end, reduces cell on the first filter membrane The accumulation of solution, it is to avoid the waste of cell solution.
This utility model is further arranged to:The arc for driving cartridge filter double swerve in the body is hinged with outside the body The first drive rod of shape, described first drive rod one end are hinged on tube wall, and the other end is contradicted with baffle plate, and described first drives Bar is provided with the position being hinged with body makes the first drive rod be provided with limit outside the connecting rod for being hinged spot wobble, the body Make the stopper slot of the first drive rod pendulum angle.
It is hinged on tube wall by adopting above-mentioned technical proposal, the first drive rod, therefore can be around being hinged with body Position rotate, as connecting rod is arranged on the first drive rod, for drive the first drive rod around pin joint rotate, by first Drive rod passes stopper slot, stirs connecting rod so that the first drive rod is moved in stopper slot, it is to avoid the first drive rod is because of itself Gravity and rotate larger angle, the first drive rod is contradicted away from position and the baffle plate of pin joint, and then connecting rod drives first When drive rod is rotated, the first drive rod also causes baffle plate to move left and right, and baffle plate setting is on cartridge filter, therefore baffle plate movement can be with So that cartridge filter is moved left and right in body, and then causes the cellular liquid in cartridge filter when filtering, cell solution is increased The speed of the first filter membrane for flowing out, reduces filtration time, while the impurity in also avoiding cell solution sticks to the first filtration The first filter membrane is blocked on film, it is ensured that cell solution is quickly leached from the first filter membrane, while the cartridge filter for rocking is avoided Cell solution is detained on the first filter membrane.
This utility model is further arranged to:Extension spring is provided between first drive rod and tube wall, it is described One end of extension spring is connected with tube wall, and the other end is fixedly connected with the first drive rod, when extension spring is in original shape There is gap between first drive rod and baffle plate during state.
By adopting above-mentioned technical proposal, the extension spring arranged between the first drive rod and tube wall, in connection Bar by the first drive rod to move away from baffle plate direction when, extension spring is stretched and has elastic force, unclamp connecting rod when, stretching First drive rod is pulled by the elastic force of spring to baffle plate direction, and then causes the first drive rod to drive baffle plate movement, extension spring Setting cause the first drive rod to drive automatically baffle plate to move, it is simple to operate, it is convenient.
This utility model is further arranged to:The baffle plate is provided with to the confession first extended away from body central direction and drives The driving plate that lever drives.
The contact area of the first drive rod and baffle plate is increased by adopting above-mentioned technical proposal, the setting of driving plate, just In the first drive rod Rapid contact driving plate and then drive baffle plate movement, the setting of driving plate avoid the first drive rod for a long time with gear Plate is contacted and causes baffle plate deformation, is unfavorable for that baffle plate is supported on body, and then ensure that cartridge filter is fixed in body.
This utility model is further arranged to:The inboard wall of tube body is provided with gathering sill, and the cartridge filter is provided with and leads To the guide pad that groove coordinates, gap is provided with when guide pad inserts gathering sill between guide pad and gathering sill and when coordinating first Drive rod drives driving plate movement.
By adopting above-mentioned technical proposal, when in cartridge filter insertion tube body, guide pad is inserted in gathering sill, it is ensured that mistake During filter cylinder insertion body, driving plate and the first drive rod are to contact, while depositing and gathering sill between during guide pad insertion gathering sill In gap, therefore guide pad does not interfere with movement of the cartridge filter in body with the cooperation to gathering sill.
This utility model is further arranged to:First drive rod is in the position with body pin joint and baffle plate contact point Between be provided with the second drive rod, when extension spring in a state of nature when there is gap between the second drive rod and body, when When one drive rod is contradicted with baffle plate, the second drive rod is contradicted with tube wall.
It is easy to when the first drive rod drives baffle plate to move by adopting the setting of above-mentioned technical proposal, the second drive rod, Second drive rod is rotated also with the first drive rod and clashes into tube wall so that tube wall produces vibration so that in body Cell solution on wall flows rapidly into its bottom, accelerates the collection of cell solution, improves the efficiency of filtration.
This utility model is further arranged to:The cover plate for cartridge filter is closed is hinged with the top of the cartridge filter.
Avoided when cell solution filters membrane filtration into first by adopting the setting of above-mentioned technical proposal, cover plate, it is miscellaneous Matter liquid is entered in cartridge filter, is caused the pollution of cell solution, is protected cell solution not to be contaminated.
This utility model is further arranged to:The tube wall is provided with graduation mark.
By adopting above-mentioned technical proposal, needed for the graduation mark arranged on tube wall is easy to operator to filter as needed Cell solution, it is to avoid many filtration cell solution, also reduce filtration time while causing the wasting of resources.
This utility model is further arranged to:The bottom of the pipe is set to cone.
The speed that cell solution flows into its bottom be increased by adopting above-mentioned technical proposal, the setting of cone, just The cell solution for completing is filtered in collecting.
This utility model is further arranged to:The point is provided with the stationary plane for extending downwardly.
It is easy to the first filter membrane is fixed on angular cut by adopting above-mentioned technical proposal, the setting of stationary plane, increases The contact area of the first filter membrane and stationary plane so that the first filter membrane is firm to be bonded on angular cut, it is ensured that cell is molten The stability that liquid is filtered.
In sum, this utility model has the advantages that:On cartridge filter on the setting of angular cut and angular cut The setting of the first filter membrane increases the filter area of cell solution, while avoid cell solution from being trapped on the first filter membrane, The setting of the first drive rod and connecting rod causes cartridge filter to move in body, accelerates shaking for cell solution on the first filter membrane It is dynamic, and then the rate of outflow is increased, improve filter efficiency.
Description of the drawings
Fig. 1 is the configuration schematic diagram of embodiment 1;
Fig. 2 is the structural representation of embodiment 1;
Fig. 3 is the structural representation of embodiment 2;
Fig. 4 is the annexation schematic diagram for embodying the first drive rod and stopper slot of embodiment 2;
Fig. 5 is the structural representation of embodiment 3;
Fig. 6 is the structural representation of embodiment 4.
In figure, 1, body;11st, cartridge filter;111st, angular cut;112nd, baffle plate;113rd, point;114th, the first filter membrane; 115th, stationary plane;116th, cover plate;117th, graduation mark;118th, driving plate;119th, the first drive rod;1191st, connecting rod;1192nd, limit Position groove;12nd, guide pad;13rd, gathering sill;14th, extension spring;15th, the second drive rod.
Specific embodiment
This utility model is described in further detail below in conjunction with accompanying drawing.
Embodiment 1:A kind of centrifuge tube with cell filter, sees figures.1.and.2, including the body 1, mistake of upper end open Filter cylinder 11,11 upper end of cartridge filter arrange to the baffle plate 112 extended away from 11 center position of cartridge filter, when cartridge filter 11 passes through When the opening of body 1 is entered in body 1, the tube wall of body 1 has supporting role to baffle plate 112, and then cartridge filter 11 is fixed In body 1, be provided with the cover plate 116 for the opening of closed tube 1 in 1 upper end of body of upper end open, cover plate 116 so that incited somebody to action Filter cylinder 11 is closed, it is to avoid when cell solution enters the first filter membrane 114 and filters, impurity liquid is entered in cartridge filter 11, causes thin The pollution of cell lysis liquid, offers an angular cut 111 on cartridge filter 11, and 11 lower end of cartridge filter forms one with angular cut 111 Point 113, is provided with the stationary plane 115 for extending downwardly in point 113, and cartridge filter 11 is provided with the position of angular cut 111 One filter membrane 114, stationary plane 115 increased the contact area of the first filter membrane 114 and cartridge filter 11, be easy to firm fix the Angular cut 111 is completely covered by one filter membrane 114, the first filter membrane 114;1 lower end of body is set to into cone, is easy to cell molten The flowing of liquid;In addition graduation mark 117 is provided with 1 outer wall of body, the cell solution needed for being easy to operator to filter as needed, Many filtration cell solution are avoided, the wasting of resources is caused.
Specific operation process:When filtration cell solution is needed, cover plate 116 is opened, cell solution is poured into cartridge filter 11 Inside hereafter cover cover plate 116 to close cartridge filter 11, as the baffle plate 112 of 11 upper end of cartridge filter is subject to the pipe walls support of body 1 Effect, therefore with the inflow of cell solution, cartridge filter 11 is fixed in body 1, cell solution is contacting the first filter membrane When 114, as the first filter membrane 114 is fixed on the angular cut 111 of filter tube, therefore cell solution can be filtered from first Filter on film 114, flow in body 1,11 lower end of cartridge filter forms a point 113, therefore cell with angular cut 111 When solution flows into 11 lower end of cartridge filter, can't pile up in 11 lower end of cartridge filter, flow out from the first filter membrane 114 successively, and then Into in body 1, during the conical lower end of stream to body 1, acquisition time, operator are saved in the quickening of the flow velocity of cell solution According to the collection cell solution of graduation mark 117 on 1 outer wall of body, it is to avoid excessive filtration cell solution, the wasting of resources is caused Also reduce filtration time simultaneously.
Embodiment 2:A kind of centrifuge tube with cell filter, the difference with embodiment 1 is, with reference to Fig. 3 and Tu 4, gathering sill 13 is provided with 1 inwall of body, be provided with the guide pad 12 coordinated with gathering sill 13, when leading on 11 outer wall of cartridge filter Be provided with gap between guide pad 12 and gathering sill 13 when gathering sill 13 is inserted to block 12, so that cartridge filter 11 is in 1 interior left and right of body Rock, first drive rod of arc 119 for making the double swerve in the body 1 of cartridge filter 11 is hinged outside body 1, first drives 119 one end of bar is hinged with 1 outer wall of body, and the other end is contradicted with baffle plate 112, in order to preferably make the first drive rod 119 drive gear Plate 112 is rocked, therefore the drive for driving is provided with to the first drive rod of confession 119 extended away from 1 center position of body on baffle plate 112 Dynamic plate 118, when guide pad 12 inserts gathering sill 13, the first drive rod 119 drives driving plate 118 to move, and 1 outer wall of body is in hinge It is provided with above the position for connecing the first drive rod 119 to the stopper slot 1192 arranged away from 1 center of body, the first drive rod 119 pass through stopper slot 1192, stopper slot 1192 limit the first drive rod 119 swing angle, it is to avoid the first drive rod 119 because Self gravitation and rotate larger angle, be not easy to adjust, the position being hinged with body 1 in the first drive rod 119 is fixed with Connecting rod 1191, connecting rod 1191 drive the first drive rod 119 around being hinged spot wobble.
Specific operation process:When cartridge filter 11 is put into body 1, in the insertion gathering sill 13 of guide pad 12 so that driving plate 118 are contacted with the first drive rod 119, and guide pad 12 is caused in 11 quick insertion body 1 of cartridge filter with the cooperation of gathering sill 13, are increased Added cartridge filter 11 with the connection of body 1 while being easy to quick positioning, stir connecting rod 1191 and the first drive rod 119 is caused in limit Move in the groove 1192 of position, when the first drive rod 119 is moved to the direction of close driving plate 118, the first drive rod 119 pairs drives Plate 118 applies and 119 moving direction identical thrust of the first drive rod, and the first drive rod 119 will drive driving plate 118 to close 1 center of body is moved, and then causes cartridge filter 11 also in body 1 as the direction of the first drive rod 119 is moved, when the first drive Lever 119 to away from driving plate 118 direction move when, driving plate 118 due to not by the first drive rod 119 thrust and Homing, therefore cartridge filter 11, also because the gravity of itself and homing, the first drive rod 119 and so on moves, makes The double swerve in the body 1 of cartridge filter 11, and then cause the cellular liquid in cartridge filter 11 when filtering, increased cell molten The speed of the first filter membrane 114 that liquid flows out, reduces filtration time, while the impurity in also avoiding cell solution sticks to first First filter membrane 114 is blocked on filter membrane 114, it is ensured that cell solution is quickly leached from the first filter membrane 114, while rocking Cartridge filter 11 avoid cell solution from being detained on the first filter membrane 114.
Embodiment 3:A kind of centrifuge tube with cell filter, the difference with embodiment 2 is, with reference to Fig. 5, the Extension spring 14 is provided between 1 outer wall of one drive rod 119 and body, one end of extension spring 14 is fixed with the first drive rod 119 Connection, the other end is fixedly connected with 1 outer wall of body, when extension spring 14 in a state of nature when the first drive rod 119 and baffle plate There is gap between 112, it is to avoid the first drive rod 119 hinders installation of the cartridge filter 11 in body 1, in connecting rod 1191 by the One drive rod 119 to move away from 112 direction of baffle plate when, extension spring 14 is stretched and has elastic force, unclamp connecting rod 1191 When, the first drive rod 119 is pulled by the elastic force of extension spring 14 to 112 direction of baffle plate, and then the first drive rod 119 is driven Baffle plate 112 is moved, and the setting of extension spring 14 causes the first drive rod 119 to drive automatically baffle plate 112 to move, easy to operate.
Embodiment 4:A kind of centrifuge tube with cell filter, the difference with embodiment 3 is, with reference to Fig. 6, first Drive rod 119 is provided with to 1 outer wall of body the second drive rod 15 for extending, and the second drive rod 15 is in the first drive rod 119 and pipe The top of 1 pin joint of body, and below the position of 112 contact point of the first drive rod 119 and baffle plate, when extension spring 14 is in original There is gap between second drive rod 15 and body 1 during beginning state, second drives when the first drive rod 119 is contacted with baffle plate 112 Lever 15 is contradicted with 1 outer wall of body, therefore when the first drive rod 119 drives baffle plate 112 to move, the second drive rod 15 also with First drive rod 119 is rotated and clashes into 1 outer wall of body so that 1 outer wall of body produces vibration, and then causes on 1 inwall of body Cell solution flows rapidly into 1 bottom of body, accelerates the collection of cell solution, improves the efficiency of filtration.
This specific embodiment is only which is not to restriction of the present utility model, ability to explanation of the present utility model Field technique personnel can make the modification for not having creative contribution after this specification is read as needed to the present embodiment, but As long as all being protected by Patent Law in right of the present utility model.

Claims (10)

1. a kind of centrifuge tube with cell filter, including the body of upper end open(1), it is characterised in that:The body(1)'s Upper end is arranged with cartridge filter(11), the cartridge filter(11)Upper end is provided with for by cartridge filter(11)It is fixed on body(1)On Baffle plate(112), the cartridge filter(11)It is provided with angular cut(111), the cartridge filter(11)Lower end and angular cut(111)Formed One point(113), the cartridge filter(11)In angular cut(111)Position be provided with covering angular cut(111)The first mistake Filter membrane(114).
2. a kind of centrifuge tube with cell filter according to claim 1, it is characterised in that:The body(1)Outer hinge It is connected to driving cartridge filter(11)In body(1)First drive rod of arc of interior double swerve(119), first drive rod (119)One end is hinged on body(1)On outer wall, the other end and baffle plate(112)Contradict, first drive rod(119)With pipe Body(1)The position being hinged is provided with makes the first drive rod(119)Around the connecting rod for being hinged spot wobble(1191), the body(1)Outward Side is provided with the first drive rod of restriction(119)The stopper slot of pendulum angle(1192).
3. a kind of centrifuge tube with cell filter according to claim 2, it is characterised in that:First drive rod (119)With body(1)Extension spring is provided between outer wall(14), the extension spring(14)One end and body(1)Outer wall connects Connect, the other end and the first drive rod(119)It is fixedly connected, works as extension spring(14)First drive rod when in a state of nature (119)With baffle plate(112)Between there is gap.
4. a kind of centrifuge tube with cell filter according to claim 3, it is characterised in that:The baffle plate(112)On It is provided with to away from body(1)The first drive rod of confession that center position extends(119)The driving plate of driving(118).
5. a kind of centrifuge tube with cell filter according to claim 4, it is characterised in that:The body(1)Inwall It is provided with gathering sill(13), the cartridge filter(11)It is provided with and gathering sill(13)The guide pad of cooperation(12), work as guide pad (12)Insertion gathering sill(13)When guide pad(12)With gathering sill(13)Between be provided with gap and coordinate when the first drive rod (119)Drive driving plate(118)It is mobile.
6. a kind of centrifuge tube with cell filter according to claim 3, it is characterised in that:First drive rod (119)With body(1)Pin joint and baffle plate(112)The second drive rod is provided between the position of contact point(15), when stretching bullet Spring(14)Second drive rod when in a state of nature(15)With body(1)Between there is gap, when the first drive rod(119)With gear Plate(112)Second drive rod during conflict(15)With body(1)Outer wall is contradicted.
7. a kind of centrifuge tube with cell filter according to claim 1, it is characterised in that:The cartridge filter(11)Top Portion is hinged with for by cartridge filter(11)The cover plate of closing(116).
8. a kind of centrifuge tube with cell filter according to claim 1, it is characterised in that:The body(1)Outer wall It is provided with graduation mark(117).
9. a kind of centrifuge tube with cell filter according to claim 1, it is characterised in that:The body(1)Lower end It is set to cone.
10. a kind of centrifuge tube with cell filter according to claim 1, it is characterised in that:The point(113) It is provided with the stationary plane for extending downwardly(115).
CN201620650132.3U 2016-06-25 2016-06-25 A kind of centrifuge tube with cell filter Active CN206052013U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201620650132.3U CN206052013U (en) 2016-06-25 2016-06-25 A kind of centrifuge tube with cell filter

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201620650132.3U CN206052013U (en) 2016-06-25 2016-06-25 A kind of centrifuge tube with cell filter

Publications (1)

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CN206052013U true CN206052013U (en) 2017-03-29

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107510986A (en) * 2017-10-13 2017-12-26 上海海洋大学 It is a kind of cut and filter and cartridge filter
CN109652308A (en) * 2018-12-20 2019-04-19 中国科学院微电子研究所 Cell sorting device
CN109932212A (en) * 2019-04-18 2019-06-25 首都医科大学附属北京安贞医院 It is centrifuged extraction element
CN111589484A (en) * 2020-06-29 2020-08-28 兰州东立龙信息技术有限公司 Side-sliding centrifuge tube for high-speed centrifuge

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107510986A (en) * 2017-10-13 2017-12-26 上海海洋大学 It is a kind of cut and filter and cartridge filter
CN109652308A (en) * 2018-12-20 2019-04-19 中国科学院微电子研究所 Cell sorting device
CN109932212A (en) * 2019-04-18 2019-06-25 首都医科大学附属北京安贞医院 It is centrifuged extraction element
CN111589484A (en) * 2020-06-29 2020-08-28 兰州东立龙信息技术有限公司 Side-sliding centrifuge tube for high-speed centrifuge

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