CN205941366U - Aquatic products freshness detection device based on FRET - Google Patents
Aquatic products freshness detection device based on FRET Download PDFInfo
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- CN205941366U CN205941366U CN201620748954.5U CN201620748954U CN205941366U CN 205941366 U CN205941366 U CN 205941366U CN 201620748954 U CN201620748954 U CN 201620748954U CN 205941366 U CN205941366 U CN 205941366U
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- histamine
- storehouse
- fret
- aquatic products
- fluorescence
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Abstract
The utility model discloses an aquatic products freshness detection device based on FRET for swiftly, the histamine of convenient detection aquatic products, combine storehouse and fluorescence response storehouse including the histamine, it switches on the passageway to be equipped with between histamine combination storehouse and the fluorescence response storehouse, it is equipped with the valve on the passageway to switch on, the histamine combines to be equipped with in the storehouse magnetic nanoparticle that can combine with the histamine, install the luminous platform of FRET in the fluorescence response storehouse, fluorescence response storehouse corresponds the luminous platform of FRET and is equipped with fluorescence detector. The utility model discloses a histamine combines the storehouse to be used for magnetic nanoparticle and histamine reagent, even histamine and magnetic nanoparticle specifically bind to get into the luminous platform of FRET in the fluorescence response storehouse between then, can the glimmering light intensity of visual test through fluorescence detector, and and then judge the concentration of histamine and the freshness of aquatic products.
Description
Technical field
The utility model is related to field of food detection and in particular to filling to a kind of detection of the aquatic products freshness based on FRET
Put.
Background technology
Histamine is a kind of low molecule organic compounds containing nitrogen with biologically active, is prevalent in varieties of food items as fermented
In product, dairy products, drinks, aquatic products and its product, especially aquatic products are the most prominent.Histamine is as a kind of chemistry in human body
Transmitter, by with cell membrane on receptor acting and play toxicity, such as eat excessive histamine, the lighter causes allergic reaction.
By the detection to histamine in aquatic products, the freshness of aquatic products can be detected, the safety of aquatic products can be detected again.At present, aquatic products
In product, the detection method of histamine mainly includes HPLC method, capillary electrophoresis, colorimetric method, biochemical sensor method, ELISA
Deng.These analysis methods are complicated, time-consuming and relatively costly.
FRET is FRET, be a kind of by intermolecular dipolar interaction, by donor excitation state
To the nonradiative transition process of acceptor excitation state, acceptor can launch the characteristic spectrum of itself to energy transfer, produces fluorescence.FRET
In composition detection experiment, there is simple to operate, visual result.
Utility model content
For the histamine of quick and easy detection aquatic products, to judge the freshness of aquatic products, the utility model provides
One kind detects histamine based on FRET, and then detects aquatic products freshness detection means.
The technical solution adopted in the utility model is as follows：
A kind of aquatic products freshness detection means based on FRET, combines storehouse including histamine and senses storehouse, described histamine with fluorescence
It is provided with conductive channel in conjunction between storehouse and fluorescence sensing storehouse, described conductive channel is provided with valve, and described histamine sets with reference in storehouse
There is the magnetic nanoparticle being combined with histamine, the luminous platform of FRET, described fluorescence sensing are installed in described fluorescence sensing storehouse
The luminous platform of the corresponding FRET in storehouse is provided with fluorescence detector.
Described fluorescence sensing storehouse is arranged on described histamine and combines below storehouse, and it is cone bottom that described histamine combines storehouse, described conducting
Passage is arranged on the lower section at cone bottom.
Described histamine is provided with the added holes of histamine reagent with reference to storehouse.
Described magnetic nanoparticle includes Fe3O4 base core, and the external engagement of described Fe3O4 base core has histamine antibody.
The luminous platform of described FRET includes nano-porous films, is furnished with Graphene quantum in the nano-pore of described nano-porous films
Point, the combined outside of described graphene quantum dot has histamine antibody.
The beneficial effects of the utility model are：
Histamine of the present utility model combines storehouse and is used for magnetic nanoparticle and histamine reagent, even if histamine and magnetic Nano
Particle specifically bind, then between enter fluorescence sense storehouse in FRET light platform, can intuitively be examined by fluorescence detector
Survey the intensity of fluorescence, and then judge the concentration of histamine and the freshness of aquatic products.
Brief description
Fig. 1 is the schematic diagram of the utility model embodiment.
Fig. 2 is the schematic diagram of magnetic nanoparticle in the utility model embodiment.
Fig. 3 is the schematic diagram after magnetic nanoparticle and histamine specific binding in the utility model embodiment.
Fig. 4 is the schematic diagram of the luminous platform of FRET in the utility model embodiment.
Histamine combines storehouse 1, fluorescence senses storehouse 2, conductive channel 3, valve 4, FRET luminous platform 5, fluorescence detector 6, magnetic
Property nano particle 7, cone bottom 11, added holes 12, nano-porous films 51, graphene quantum dot 52, Fe3O4 base core 71, histamine reagent
01st, histamine antibody 02.
Specific embodiment
The utility model is described in further detail with embodiment below in conjunction with the accompanying drawings.
In embodiment, as shown in figure 1, a kind of aquatic products freshness detection means based on FRET, including histamine combine storehouse 1 with
Fluorescence senses storehouse 2, and described histamine is provided with conductive channel 3 with reference between storehouse 1 and fluorescence sensing storehouse 2, and described conductive channel 3 is provided with
Valve 4, described histamine, with reference to being provided with the magnetic nanoparticle 7 being combined with histamine in storehouse 1, is installed in described fluorescence sensing storehouse 2
FRET is had to light platform 5, the corresponding FRET in described fluorescence sensing storehouse 2 platform 5 that lights is provided with fluorescence detector 6.The group of the present embodiment
Amine combines storehouse 1 and is used for magnetic nanoparticle 7 and histamine reagent 01, even if histamine is specifically bound with magnetic nanoparticle 7, so
Enter the luminous platform 5 of FRET that fluorescence senses in storehouse 2 between afterwards, by the intensity of fluorescence detector 6 energy visual inspection fluorescence, enter
And judge the concentration of histamine and the freshness of aquatic products.
In embodiment, as shown in figure 1, described fluorescence sensing storehouse 2 is arranged on described histamine combining below storehouse 1, described histamine
It is cone bottom 11 in conjunction with storehouse 1, described conductive channel 3 is arranged on the lower section at cone bottom 11.The present embodiment structure, with histamine specific binding
The magnetic nanoparticle 7 finishing directly can enter the luminous platform 5 of FRET by conductive channel 3, completes FRET detection process, inspection
The survey cycle is shorter, and operation is simpler.
In embodiment, as shown in figure 1, described histamine is provided with the added holes 12 of histamine reagent 01 with reference to storehouse 1.The present embodiment
Added holes 12 be located at histamine combine storehouse 1 top, facilitate the interpolation of histamine reagent 01.
In embodiment, as shown in Figure 2 and Figure 3, described magnetic nanoparticle 7 includes Fe3O4 base core 31, described Fe3O4 base core
31 external engagement has histamine antibody 02.The present embodiment, Fe3O4 base core 31 also can be selected for Fe3O4@Au nano particle, and outside connects
Conjunction has histamine antibody 02, and histamine antibody 02 is used for being combined with histamine.
In embodiment, as shown in figure 4, described FRET lights, platform 5 includes nano-porous films 51, described nano-porous films 51
Nano-pore in be furnished with graphene quantum dot 52, the combined outside of described graphene quantum dot 52 has histamine antibody 02.This enforcement
Histamine antibody 02 on the graphene quantum dot 52 of example and magnetic nanoparticle 7 occur produce during FRET
Fluorescence, detects this FRET fluorescence radiation Strength Changes, just can judge Histamine concentrations size in different storage period marine products, and then
Disclose the change procedure of marine product quality safety.
Obviously, above-described embodiment of the present utility model is merely to illustrate that the utility model example, and not
Restriction to embodiment of the present utility model.For those of ordinary skill in the field, on the basis of described above
On can also make change or the variation of other multi-forms.There is no need to poor example is given to all of embodiment.
And these belong to the obvious change that connotation of the present utility model amplified out or change and still fall within the utility model
Protection domain.
Claims (5)
1. a kind of aquatic products freshness detection means based on FRET it is characterised in that：Combine storehouse including histamine（1）With fluorescence sensing
Storehouse（2）, described histamine combines storehouse（1）Sense storehouse with fluorescence（2）Between be provided with conductive channel（3）, described conductive channel（3）On set
There is valve（4）, described histamine combines storehouse（1）Inside it is provided with the magnetic nanoparticle being combined with histamine（7）, described fluorescence sensing storehouse
（2）The luminous platform of FRET is inside installed（5）, described fluorescence sensing storehouse（2）Correspond to the luminous platform of FRET（5）It is provided with fluorescence detector
（6）.
2. the aquatic products freshness detection means based on FRET according to claim 1 it is characterised in that：Described fluorescence sensing
Storehouse（2）It is arranged on described histamine and combine storehouse（1）Lower section, described histamine combines storehouse（1）For boring bottom（11）, described conductive channel（3）
It is arranged on cone bottom（11）Lower section.
3. the aquatic products freshness detection means based on FRET according to claim 1 it is characterised in that：Described histamine combines
Storehouse（1）It is provided with histamine reagent（01）Added holes（12）.
4. the aquatic products freshness detection means based on FRET according to claim 1 it is characterised in that：Described magnetic Nano
Particle（7）Including Fe3O4 base core（71）, described Fe3O4 base core（71）External engagement have histamine antibody（02）.
5. the aquatic products freshness detection means based on FRET according to claim 1 it is characterised in that：Described FRET lights
Platform（5）Including nano-porous films（51）, described nano-porous films（51）Nano-pore in be furnished with graphene quantum dot（52）, institute
State graphene quantum dot（52）Combined outside have histamine antibody（02）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620748954.5U CN205941366U (en) | 2016-11-08 | 2016-11-08 | Aquatic products freshness detection device based on FRET |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620748954.5U CN205941366U (en) | 2016-11-08 | 2016-11-08 | Aquatic products freshness detection device based on FRET |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107421937A (en) * | 2017-08-29 | 2017-12-01 | 浙江工业大学 | The histamine detection method of FRET biology sensor based on magnetic nano particle separation |
| CN110618116A (en) * | 2019-08-28 | 2019-12-27 | 江苏大学 | Preparation method and application of intelligent indication label for visually detecting freshness of meat |
-
2016
- 2016-11-08 CN CN201620748954.5U patent/CN205941366U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107421937A (en) * | 2017-08-29 | 2017-12-01 | 浙江工业大学 | The histamine detection method of FRET biology sensor based on magnetic nano particle separation |
| CN110618116A (en) * | 2019-08-28 | 2019-12-27 | 江苏大学 | Preparation method and application of intelligent indication label for visually detecting freshness of meat |
| CN110618116B (en) * | 2019-08-28 | 2022-01-11 | 江苏大学 | Preparation method and application of intelligent indication label for visually detecting freshness of meat |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170208 Termination date: 20211108 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |