CN205865772U - Freezing carrier of preserving of oocyte and embryonal glass ization - Google Patents

Freezing carrier of preserving of oocyte and embryonal glass ization Download PDF

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Publication number
CN205865772U
CN205865772U CN201620630229.8U CN201620630229U CN205865772U CN 205865772 U CN205865772 U CN 205865772U CN 201620630229 U CN201620630229 U CN 201620630229U CN 205865772 U CN205865772 U CN 205865772U
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CN
China
Prior art keywords
straw
suction pipe
oocyte
carrier
freezing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201620630229.8U
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Chinese (zh)
Inventor
曹新燕
许保增
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Institute Special Animal and Plant Sciences CAAS
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Institute Special Animal and Plant Sciences CAAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Priority to CN201620630229.8U priority Critical patent/CN205865772U/en
Application granted granted Critical
Publication of CN205865772U publication Critical patent/CN205865772U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

Freezing carrier of preserving of oocyte and embryonal glass ization belongs to procreation biology technical field. The utility model relates to a solve present germ cell and preserve the carrier because the structure is complicated, appear loosely when certain parts, cause the problem that the cell loses easily. It includes straw, top stopper and sealed lid, the last port of top stopper sealing connection straw, and the bottom of straw is needle -like, is provided with a ring section of thick bamboo of taking the cavity on first section the lateral wall of straw, sets up the metal block in the ring section of thick bamboo, and straw inner chamber and the ring cavity air vent on through the straw lateral wall communicates with each other, through threaded connection, the straw bottom is located sealed lid between a sealed lid and the ring section of thick bamboo. The utility model is used for freezing oocyte and embryonal glass ization are freezing preserves.

Description

The vitrificated cryopreserration carrier of oocyte and embryo
Technical field
This utility model relates to the vitrificated cryopreserration carrier of oocyte and embryo, belongs to biology of reproduction technology neck Territory.
Background technology
Oocyte, embryo vitrifying freeze Techniques of preserving are the most effectual ways preserving sexual cell at present, and it is not only The continuity of rare species can be ensured, moreover it is possible to ensure diversity of organism.In the society of Modern High-Speed development, for uncommon Hope and postpone child's date of birth, and need to preserve in advance all demands of sexual cell for suffering from the Mr. and Mrs of disease, will be raw Cell colonization carries out freezen protective and all has great importance.
During sexual cell carries out freezen protective, it is female thin that the design of vitrificated cryopreserration carrier directly affects ovum The freezen protective efficiency of born of the same parents and embryo.Current conventional micro drop method preservation carrier is few due to the freezing liquid comprised such that it is able to fall Low cytotoxicity, is the most just widely used.But it is complicated, at process of cryopreservation because forming structure that this microdroplet preserves carrier In, not only can increase the probability of operator's error;And after carrier puts into liquid nitrogen, also it is easily caused all parts loose, Cause cell loss.
Summary of the invention
This utility model purpose be in order to solve existing sexual cell preserve carrier due to structure complicated, when certain parts goes out Problem that is existing loose, that easily cause cell loss, it is provided that the vitrificated cryopreserration carrier of a kind of oocyte and embryo.
The vitrificated cryopreserration carrier of oocyte described in the utility model and embryo, it includes that suction pipe, top are filled in and close Capping,
Top plug is tightly connected the upper port of suction pipe, and the bottom of suction pipe is needle-like,
Be provided with the ring cylinder of band cavity on the lateral wall of the upper semisection of suction pipe, metal derby be set in ring cylinder, suction pipe inner chamber with Ring cylinder cavity is communicated by the passage on sidewall of suction pipe;
Sealing and be threaded connection between lid and ring cylinder, suction pipe bottom is positioned at sealing lid.
Described metal derby is aluminium block or Al alloy block.
Advantage of the present utility model: this utility model adsorbs sexual cell by suction pipe is carried out evacuation, then seals After suction pipe, preservation carrier is sunk to liquid nitrogen and carry out freezen protective.This utility model has carried out totally-enclosed preservation to sample, prevents sample Product contact with liquid nitrogen, it is to avoid the potential threat that sample is contaminated.
This utility model is after sinking to liquid nitrogen, and due to the effect of expanding with heat and contract with cold of metal derby, volume reduces, can increase further Vacuum in suction pipe, makes sexual cell be adsorbed in the aspiration port of suction pipe reliably, reduces freezing oocyte and embryo Loss probability.In addition the heat conductivility of metal derby, contributes to the quick freezing of Seal and preservation sample, reduces the freezing damage of sample Wound.And, the structure of this utility model preservation carrier arranges and facilitates operator more quickly to take out cell from liquid nitrogen, improves Freezen protective efficiency.
Ring cylinder threadedly realizes being tightly connected with sealing lid, and connected mode is simply effective, connects reliable for effect, also It is prevented from connection procedure the vibration effect that sexual cell is caused, efficiently solves and prior art easily causes cell lose The problem lost.This utility model has simple in construction, the convenient advantage being suitable for, and is a kind of preferably biological material vitrified frozen Preserve carrying tool.
Accompanying drawing explanation
Fig. 1 is the structural representation of the vitrificated cryopreserration carrier of oocyte described in the utility model and embryo.
Detailed description of the invention
Detailed description of the invention one: present embodiment is described below in conjunction with Fig. 1, oocyte and embryo described in present embodiment Vitrificated cryopreserration carrier, it include suction pipe 1, top plug 2 and seal lid 3,
Top plug 2 is tightly connected the upper port of suction pipe 1, and the bottom of suction pipe 1 is needle-like,
It is provided with the ring cylinder 1-1 of band cavity on the lateral wall of the upper semisection of suction pipe 1, in ring cylinder 1-1, metal derby 1-2 is set, Suction pipe 1 inner chamber is communicated by the passage 1-3 on suction pipe 1 sidewall with ring cylinder 1-1 cavity;
Sealing and be threaded connection between lid 3 and ring cylinder 1-1, suction pipe 1 bottom is positioned at sealing lid 3.
In present embodiment, suction pipe 1, top plug 2 and sealing lid 3 define a carrier tool being tightly connected, ring cylinder 1-1 Setting holding position when can move as carrier;When metal derby 1-2 volume-diminished at low temperatures, the thin sky in suction pipe Gas can be circulated by passage, thus contributes to the air-flow equilibrium in suction pipe, and the adsorption effect making sexual cell is more preferable.
Detailed description of the invention two: embodiment one is described further by present embodiment, described metal derby 1-2 is aluminium block Or Al alloy block.
The thermal linear expansion coefficient of aluminum metal is relatively big, which dictates that its effect expanded with heat and contract with cold becomes apparent from, thus effectively Reduce the pressure in suction pipe.

Claims (1)

1. an oocyte and the vitrificated cryopreserration carrier of embryo, it is characterised in that it includes suction pipe (1), top plug (2) (3) are covered with sealing,
Top plug (2) is tightly connected the upper port of suction pipe (1), and the bottom of suction pipe (1) is needle-like,
It is provided with the ring cylinder (1-1) of band cavity on the lateral wall of the upper semisection of suction pipe (1), in ring cylinder (1-1), metal derby (1-is set 2), suction pipe (1) inner chamber is communicated by the passage (1-3) on suction pipe (1) sidewall with ring cylinder (1-1) cavity;
Sealing and be threaded connection between lid (3) and ring cylinder (1-1), suction pipe (1) bottom is positioned at sealing lid (3);
Described metal derby (1-2) is aluminium block or Al alloy block.
CN201620630229.8U 2016-06-23 2016-06-23 Freezing carrier of preserving of oocyte and embryonal glass ization Expired - Fee Related CN205865772U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201620630229.8U CN205865772U (en) 2016-06-23 2016-06-23 Freezing carrier of preserving of oocyte and embryonal glass ization

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201620630229.8U CN205865772U (en) 2016-06-23 2016-06-23 Freezing carrier of preserving of oocyte and embryonal glass ization

Publications (1)

Publication Number Publication Date
CN205865772U true CN205865772U (en) 2017-01-11

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201620630229.8U Expired - Fee Related CN205865772U (en) 2016-06-23 2016-06-23 Freezing carrier of preserving of oocyte and embryonal glass ization

Country Status (1)

Country Link
CN (1) CN205865772U (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110392527A (en) * 2017-03-10 2019-10-29 Imv技术股份有限公司 The vitrification system and method for biological substance
CN113016776A (en) * 2019-12-24 2021-06-25 上海明悦医疗科技有限公司 Carrier, vacuumizing device and tissue cryopreservation system

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110392527A (en) * 2017-03-10 2019-10-29 Imv技术股份有限公司 The vitrification system and method for biological substance
CN110392527B (en) * 2017-03-10 2021-12-03 Imv技术股份有限公司 Biological material vitrification system and method
CN113016776A (en) * 2019-12-24 2021-06-25 上海明悦医疗科技有限公司 Carrier, vacuumizing device and tissue cryopreservation system

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GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20170111

Termination date: 20170623