CN205861688U - A kind of papery ELISA kit detecting Car T cell IFN γ release - Google Patents

Abstract

The utility model discloses a kind of papery ELISA kit detecting Car T cell IFN γ release, including box body, the inner chamber of described box body is disposed with standard QC from left to right, treatment fluid pipe and cleanout fluid pipe, the right side of described cleanout fluid pipe is respectively arranged with an anti-pipe and two anti-pipes, and two anti-pipe be positioned at the lower section of an anti-pipe, the right side of described two anti-pipes is provided with stop buffer pipe, the TMB that is provided above of described stop buffer pipe shows liquid pipe, the inner chamber of described box body is respectively arranged with aseptic independent packaging and cultivates orifice plate and lucifuge independent packaging covering antigen connection orifice plate, described lucifuge independent packaging covers the right side of antigen connection orifice plate and is provided with 96 orifice plates covering offset plate films.The papery ELISA kit of this detection Car T cell IFN γ release, by the improvement to box body, for Car T cell the factor discharge for, the most practical succinctly, simultaneously provide co-culture of cells program can more science, exactly detect Car T cell IFN γ burst size.

Description

A kind of papery ELISA kit detecting the release of Car-T cell IFN-γ

Technical field

This utility model relates to reagent field of immunology, a kind of papery detecting the release of Car-T cell IFN-γ ELISA kit.

Background technology

In field of biological pharmacy, one of product that test kit is indispensable in becoming industry as the instrument of loaded reagent, Test kit is especially applied to ELISA antibody test, and ELISA test is a kind of sensitivity height, high specificity, reproducible Laboratory diagnostic methods, due to its stable reagent, easily preserve, easy and simple to handle, result judges the factors such as more objective, have been widely used In immunologic each field, one of performance that Car-T is successfully prepared, is that it may result in the release of cytokines of relative high levels, IFN-γ is one in these cytokines, and its burst size can effectively assess the function of Car-T, at the cell of assessment Car-T Before factor emission levels, need first Car-T cell and tumor cell to be co-cultured a period of time, then detecting factor burst size, mesh Before there is the ELISA detection kit of many people's IFN-γ on the market, principle is basically identical, people's IFN-γ of such as Takara ELISA kit, is developed the color by antigen antibody reaction associating TMB and detects the content of IFN-γ, but the standard of these test kits Product diluted concentration is on the low side for the IFN-γ burst size relative to Car-T, provides co-culture of cells material the most simultaneously.

Utility model content

The purpose of this utility model is to provide a kind of papery ELISA kit detecting the release of Car-T cell IFN-γ, With the problem solving to propose in above-mentioned background technology.

For achieving the above object, this utility model provides following technical scheme: a kind of detection Car-T cell IFN-γ discharges Papery ELISA kit, including box body, the inner chamber of described box body is disposed with standard QC, treatment fluid pipe from left to right With cleanout fluid pipe, the right side of described cleanout fluid pipe is respectively arranged with an anti-pipe and two anti-pipes, and two anti-pipes are positioned under an anti-pipe Side, the right side of described two anti-pipes is provided with stop buffer pipe, and the TMB that is provided above of described stop buffer pipe shows liquid pipe, described box The inner chamber of body is respectively arranged with aseptic independent packaging and cultivates orifice plate and lucifuge independent packaging covering antigen connection orifice plate, and described lucifuge is only Vertical packaging covers the right side of antigen connection orifice plate and is provided with 96 orifice plates covering offset plate films, and described 96 orifice plates cover offset plate film Right side be provided with 96 orifice plate framves, the right side of described 96 orifice plate framves is provided with lucifuge bag, described aseptic independent packaging The bottom cultivating orifice plate is fixedly connected with aseptic independent packaging culture hole grillage.

Preferably, the quantity of described standard QC, treatment fluid pipe and cleanout fluid pipe is two.

Preferably, described lucifuge independent packaging covering antigen connection orifice plate is positioned at the right side of aseptic independent packaging cultivation orifice plate.

Preferably, the quantity that described 96 orifice plates cover offset plate film is two.

Preferably, it is 96 that the quantity of orifice plate is cultivated in described aseptic independent packaging, and is uniformly distributed.

Compared with prior art, the beneficial effects of the utility model are: the papery of this detection Car-T cell IFN-γ release ELISA kit, by the improvement to box body, for the factor of Car-T cell discharges, the most practical succinctly, simultaneously There is provided co-culture of cells program can more science, exactly detect Car-T cell IFN-γ burst size.

Accompanying drawing explanation

Fig. 1 is this utility model structural representation；

Fig. 2 is the sectional view of this utility model structure；

Fig. 3 is the structural representation that orifice plate is cultivated in the aseptic independent packaging of this utility model.

In figure: 1 box body, 2 standard QCs, 3 treatment fluid pipes, 4 cleanout fluid pipes, 5 one anti-pipes, 6 two anti-pipes, 7TMB show liquid Orifice plate, 10 lucifuge independent packagings covering antigen connection orifice plate, 11 96 orifice plates are cultivated in pipe, 8 stop buffer pipes, 9 aseptic independent packagings Cover offset plate film, 12 96 orifice plate framves, 13 lucifuge bags, 14 aseptic independent packaging culture hole grillages.

Detailed description of the invention

Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is carried out Clearly and completely describe, it is clear that described embodiment is only a part of embodiment of this utility model rather than whole Embodiment.Based on the embodiment in this utility model, those of ordinary skill in the art are not under making creative work premise The every other embodiment obtained, broadly falls into the scope of this utility model protection.

Refer to Fig. 1-3, this utility model provides a kind of technical scheme: a kind of detect the release of Car-T cell IFN-γ Papery ELISA kit, including box body 1, the inner chamber of box body 1 is disposed with standard QC 2, treatment fluid pipe 3 and from left to right Cleanout fluid pipe 4, the quantity of standard QC 2, treatment fluid pipe 3 and cleanout fluid pipe 4 is two, and the right side of cleanout fluid pipe 4 sets respectively Being equipped with anti-pipe 5 and two anti-pipes 6, and two anti-pipes 6 are positioned at the lower section of an anti-pipe 5, the right side of two anti-pipes 6 is provided with stop buffer pipe 8, The TMB that is provided above of stop buffer pipe 8 shows liquid pipe 7, and the inner chamber of box body 1 is respectively arranged with aseptic independent packaging and cultivates orifice plate 9 Covering antigen connection orifice plate 10 with lucifuge independent packaging, lucifuge independent packaging covers antigen connection orifice plate 10 and is positioned at aseptic independent packaging training Supporting the right side of orifice plate 9, it is 96 that the quantity of orifice plate 9 is cultivated in aseptic independent packaging, and is uniformly distributed, and lucifuge independent packaging is covered The right side of lid antigen connection orifice plate 10 is provided with 96 orifice plates and covers the right side of offset plate film 11,96 orifice plate covering offset plate film 11 The quantity being provided with 96 orifice plate frame 12,96 orifice plates covering offset plate films 11 is two, the right side of 96 orifice plate framves 12 Being provided with lucifuge bag 13, aseptic independent packaging is cultivated the bottom of orifice plate 9 and is fixedly connected with aseptic independent packaging culture hole grillage 14, The papery ELISA kit of this detection Car-T cell IFN-γ release, by the improvement to box body 1, for Car-T cell For factor release, the most practical succinctly, provide simultaneously co-culture of cells program can more science, detect Car-exactly The IFN-γ burst size of T cell.

Embodiment the most of the present utility model, for the ordinary skill in the art, It is appreciated that in the case of without departing from principle of the present utility model and spirit and these embodiments can be carried out multiple change, repair Changing, replace and modification, scope of the present utility model is defined by the appended claims and the equivalents thereof.

Claims (5)

1. detect a papery ELISA kit for Car-T cell IFN-γ release, including box body (1), it is characterised in that: institute State the inner chamber of box body (1) and be disposed with standard QC (2), treatment fluid pipe (3) and cleanout fluid pipe (4) from left to right, described clearly The right side of lotion pipeline (4) is respectively arranged with an anti-pipe (5) and two anti-pipes (6), and two anti-pipes (6) are positioned at the lower section of an anti-pipe (5), The right side of described two anti-pipes (6) is provided with stop buffer pipe (8), and the TMB that is provided above of described stop buffer pipe (8) shows liquid pipe (7), the inner chamber of described box body (1) is respectively arranged with aseptic independent packaging and cultivates orifice plate (9) and lucifuge independent packaging covering antigen Connection orifice plate (10), described lucifuge independent packaging covers the right side of antigen connection orifice plate (10) and is provided with 96 orifice plates covering offset plate films (11) right side that, described 96 orifice plates cover offset plate film (11) is provided with 96 orifice plate framves (12), described 96 orifice plates The right side of frame (12) is provided with lucifuge bag (13), and described aseptic independent packaging is cultivated the bottom of orifice plate (9) and is fixedly connected with aseptic Independent packaging culture hole grillage (14).

A kind of papery ELISA kit detecting the release of Car-T cell IFN-γ the most according to claim 1, its feature It is: the quantity of described standard QC (2), treatment fluid pipe (3) and cleanout fluid pipe (4) is two.

A kind of papery ELISA kit detecting the release of Car-T cell IFN-γ the most according to claim 1, its feature It is: described lucifuge independent packaging covers antigen connection orifice plate (10) and is positioned at the right side of aseptic independent packaging cultivation orifice plate (9).

A kind of papery ELISA kit detecting the release of Car-T cell IFN-γ the most according to claim 1, its feature It is: the quantity that described 96 orifice plates cover offset plate film (11) is two.

A kind of papery ELISA kit detecting the release of Car-T cell IFN-γ the most according to claim 1, its feature It is: it is 96 that the quantity of orifice plate (9) is cultivated in described aseptic independent packaging, and is uniformly distributed.