CN205814714U - The supertension inactivation equipment of pathogen in liquid bio goods - Google Patents
The supertension inactivation equipment of pathogen in liquid bio goods Download PDFInfo
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- CN205814714U CN205814714U CN201620464243.5U CN201620464243U CN205814714U CN 205814714 U CN205814714 U CN 205814714U CN 201620464243 U CN201620464243 U CN 201620464243U CN 205814714 U CN205814714 U CN 205814714U
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Abstract
This utility model relates to physical method and processes the technology of liquid biological product, is specifically related to a kind of to the supertension inactivation equipment of pathogen in liquid bio goods.Including ultrahigh pressure vessel and the super-pressure pump that connects with ultrahigh pressure vessel, the water inlet end of the outlet ultrahigh pressure vessel of super-pressure pump, the outlet of the inlet communication cooling-water machine of super-pressure pump, the water side of the water inlet connection ultrahigh pressure vessel of cooling-water machine.The beneficial effects of the utility model are: blood products or life virus culture fluid are placed in ultrahigh pressure vessel, by pressurization, pressurize, the repeated multiple times effect of technical process of moment pressure release, utilize the crunch inside and outside virion poor, viromembrane capsid is made to rupture, DNA effusion and complete virion can be kept, so that it is guaranteed that protein active enzyme keeps constant while virus loses activity, the technique of this utility model inactivation of bacterial virus compared with simple, cost is relatively low, the physical method using supertension processes, and has efficiency height and inactivation of viruses feature thoroughly.
Description
Technical field
This utility model relates to physical method and processes the technology of liquid biological product, is specifically related to a kind of to liquid bio system
The supertension inactivation equipment of pathogen in product.
Background technology
Blood is commonly present the viruses such as HBV, HCV, HIV, when making blood products and vaccine, medically generally by anti-
The means such as body screening, drug purification, pasteurization, actinic irradiation are killed virus and keep its immunity, thus keep blood
Liquid goods or vaccine non-infectious.The shortcoming of the means of above-mentioned inactivation of viruses is exactly cumbersome, and cost is the highest.Due to blood
In the antibody of some virus can not occur in time and viral gene sudden change, conventional sense means can not detect virus, due to
Conventional inactivation means are cumbersome, and cost is the highest, if do not processed this part blood, for the people transfused blood or use
The people of blood products or vaccine is possible to be infected.
Applicant Senmiao Biological Engineering Technology Co., Ltd., Tianjin City was application on 06 29th, 2003
200310119362.4 " ultra high pressure treatment blood plasma makes the method for inactivation of virus ", disclose the plastics that blood plasma vacuum is enclosed sterilization
In Dai, placing it in ultrahigh pressure vessel ,-10～10 DEG C are carried out under cryogenic, are filled with room temperature liquid in ultrahigh pressure vessel
Body medium, will improve the temperature of blood bag.
Applicant Tianjin Huatai Senmiao Bioengineering Technology Co., Ltd. was application on 05 18th, 2011
201110128847.4 " methods that the low-temperature quick-freezing of blood plasma combines with supertension inactivation of virus ", disclose only residing for blood plasma
Ambient temperature, blood bag be placed on-22～-25 DEG C under conditions of pre-cooling, cool down 20～30 minutes, the plasma bags after pre-cooling put into
In ultrahigh pressure vessel with the ultrahigh pressure treatment equipment of fast cooling device；Use the method, be often filled with in ultrahigh pressure vessel
Geothermal liquid medium, will improve the temperature of blood bag.
Applicant Senmiao Biological Engineering Technology Co., Ltd., Tianjin City has applied for 03130566.0 on 08 15th, 2003
" integrated superhigh pressure biological treating apparatus ", the piston rod disclosing extremely-high tension generator is directly deep in supertension loader
Pressurization, then carries out freezing, utilizes homoiothermic water jacket to cool itself supertension loader, this cooling method, knot
Structure is complicated, lowers the temperature the material in supertension loader, and required time is long.
Applicant Tang Xieming has applied for 200420028023.5 " ultra high-pressure virus inactivating devices " on 06 17th, 2004,
Disclosing super-pressure pump and be passed through ultrahigh pressure vessel, the closure part of ultrahigh pressure vessel uses the bottom of end cover to have Flos Mume lobed
Tooth, exports with the Flos Mume lobed of liquid cylinder upper end and is slidably matched, by rotating to an angle, make end cover be flush-mounted in annular
Sealing in cavity card, this sealing means, be difficult to seal sternly, owing to ultrahigh pressure vessel is intrinsic pressure very big, end cover needs
Intensity is the highest, and manufacturing cost is big.
" the superelevation of pathogen in biological product that the applicant applied for 201410433271.6 on 08 29th, 2014
Pressure ablation method and application ", disclose the environmental conditions such as the temperature residing for detailed biological product, pressure.
The fluid media (medium) that above-mentioned patent applies supertension for biological product is all that room temperature enters in container, this situation
Under, owing to fluid media (medium) cooling needs release energy, the most just improve the temperature of biological product, and fluid media (medium) and life
Tetramune heat exchange, its variations in temperature is not easily controlled；Above-mentioned patent all fails to disclose how the temperature in holding ultrahigh pressure vessel
Degree, does not accounts for repeatedly boosting, the problem that biological product heat up with fluid media (medium).
Summary of the invention
The problem existed for prior art, this utility model provides and a kind of surpasses pathogen in liquid bio goods
High pressure inactivation equipment, while effectively inactivateing multiple pathogens, can effectively keep effective ingredient activity in biological product.
This utility model is by the following technical solutions: the supertension inactivation equipment of pathogen in liquid bio goods, including
Ultrahigh pressure vessel and the super-pressure pump connected with ultrahigh pressure vessel, the water inlet end of the outlet ultrahigh pressure vessel of super-pressure pump,
The outlet of the inlet communication cooling-water machine of super-pressure pump, the water side of the water inlet connection ultrahigh pressure vessel of cooling-water machine.
Pipeline between supertension delivery side of pump and the water inlet end of ultrahigh pressure vessel is provided with check valve, super-pressure pump
Pipeline between import and the outlet of cooling-water machine is provided with check valve.
The water inlet end of described ultrahigh pressure vessel and water side are respectively water inlet port and water outlet port, the supertension of three-way valve
The intake-outlet of container connects with the another port of three-way valve.
Described ultrahigh pressure vessel is the cylinder body being provided with column blind hole, and inferior part of the cylinder block accommodates ice-cold liquid and biological product bag
Position sets collet, and collet coordinates with cylinder gap, sets plunger rod, set close between plunger rod and column blind hole in column blind hole
Seal ring, cylinder body is fixed on base, and plunger rod is fixed on the piston rod of the first hydraulic cylinder, and the first hydraulic cylinder is fixed on door type frame
On, door type frame is fixed with the second hydraulic cylinder, the piston rod of the second hydraulic cylinder is fixed with U-shaped piece.
Described base and door type frame relative movement.
Described base is connected with frame by guide rail, and door type frame is fixed in frame, base and the piston of the 3rd hydraulic cylinder
Bar is fixing to be connected, and the 3rd hydraulic cylinder is fixed in frame.
Described base is fixed in frame, and door type frame is connected with frame by guide rail, door type frame and the work of the 3rd hydraulic cylinder
Stopper rod is fixing to be connected, and the 3rd hydraulic cylinder is fixed in frame.
The beneficial effects of the utility model are: blood products or life virus culture fluid are placed in ultrahigh pressure vessel, logical
Cross the repeated multiple times effect of technical process of pressurization, pressurize, moment pressure release, utilize the crunch inside and outside virion poor, make virus
Film capsid ruptures, DNA effusion and can keep complete virion, so that it is guaranteed that protein active while virus loses activity
Enzyme keeps constant, the technique of this utility model inactivation of bacterial virus compared with simple, cost is relatively low, use at the physical method of supertension
Reason, has efficiency height and inactivation of viruses feature thoroughly.
Accompanying drawing explanation
Fig. 1 is structural principle schematic diagram of the present utility model.
Detailed description of the invention
As it is shown in figure 1, the supertension inactivation equipment of pathogen in liquid bio goods, including ultrahigh pressure vessel 3 and with super
The super-pressure pump 14 of high-pressure bottle 3 connection, the outlet 19 of super-pressure pump 14 connects the water inlet end of ultrahigh pressure vessel 3, super-pressure pump
The import 16 of 14 connects the outlet 17 of cooling-water machine 15, and the water inlet 18 of cooling-water machine 15 connects the water side of ultrahigh pressure vessel 3.
Pipeline between outlet 19 and the water inlet end of ultrahigh pressure vessel 3 of super-pressure pump 14 is provided with check valve, superelevation
Pipeline between import 16 and the outlet 17 of cooling-water machine 15 of press pump 14 is provided with check valve.The water inlet of ultrahigh pressure vessel 3
End and water side are respectively water inlet port and the water outlet port of three-way valve 20, the intake-outlet 21 of ultrahigh pressure vessel 3 and three-way valve
The another port connection of 20.Ultrahigh pressure vessel 3 is the cylinder body being provided with column blind hole 22, and inferior part of the cylinder block accommodates ice-cold liquid and biology
The position of product bag sets collet 25, and collet 25 coordinates with cylinder gap, sets plunger rod 6 in column blind hole 22, plunger rod 6 with
Setting sealing ring 4 between column blind hole 22, cylinder body is fixed on base 2, and plunger rod 6 is fixed on the piston rod of the first hydraulic cylinder 5,
First hydraulic cylinder 5 is fixed on door type frame 12, door type frame 12 is fixed with the second hydraulic cylinder 8, on the piston rod of the second hydraulic cylinder 8
It is fixed with U-shaped piece 7.
If the U mouth width of U-shaped piece 7 is a, a diameter of d of the piston rod of the first hydraulic cylinder 5, a diameter of D of plunger rod 6, then
D≤a ＜ D.
Base 2 and door type frame 12 relative movement.Base 2 is connected with frame 1 by guide rail 13, and door type frame 12 is fixed on frame
On 1, base 2 is fixing with the piston rod of the 3rd hydraulic cylinder 10 to be connected, and the 3rd hydraulic cylinder 10 is fixed in frame 1；Or base 2 is solid
Being scheduled in frame 1, door type frame 12 is connected with frame 1 by guide rail 13, and the piston rod of door type frame 12 and the 3rd hydraulic cylinder 10 is fixed
Connecting, the 3rd hydraulic cylinder 10 is fixed in frame 1, and the 3rd hydraulic cylinder 10 is connected with petrol station II 11 by hydraulic pump II 9.
Super-pressure pump 14 includes that Master cylinder body 23, Master cylinder body 23 two ends are symmetrically arranged with subsidiary cylinder 24, Master cylinder body 23 and secondary cylinder
Being provided with piston in body 24, piston is connected by piston rod, and Master cylinder body 23 is connected with petrol station I by hydraulic pump I, subsidiary cylinder
Being provided with import 16 and outlet 19 on 24, Master cylinder body 23 connects reversal valve 25.
The operation principle of super-pressure pump is: Master cylinder body 23 is divided into left cavity and right cavity by piston, adjusts reversal valve, makes liquid
Force feed enters in left cavity, promotes piston rod to move right, the ice-cold liquid in the subsidiary cylinder on right side is injected high-pressure-resistant vessel
In, the most ice-cold liquid, by the subsidiary cylinder on the left of the import entrance of left side subsidiary cylinder, makes hydraulic oil enter in right cavity, pushes away
Dynamic piston rod is moved to the left, and is injected in high-pressure-resistant vessel by the ice-cold liquid in the subsidiary cylinder in left side, and the most ice-cold liquid passes through
In subsidiary cylinder on the right side of the import entrance of right side subsidiary cylinder.
The work process of this equipment is as follows: processed liquid bio goods vacuum is enclosed aseptic plastic bag and puts into super
In high-pressure bottle, start the 3rd hydraulic cylinder and move door type frame or base so that the column blind hole of ultrahigh pressure vessel and plunger rod pair
Just, start in the column blind hole that plunger rod is stretched into ultrahigh pressure vessel by the first hydraulic cylinder, start the second hydraulic cylinder by U-shaped piece of movement
To between plunger rod and door type frame, start super-pressure pump, in ultrahigh pressure vessel, inject ice-cold liquid.
To the supertension ablation method of pathogen in liquid bio goods:Processed liquid bio goods insert vacuum
In Dai；2. vacuum bag is forced into 200～300MPa, and keeps 1～5 minute；3. normal pressure it is down to；4. circulate above-mentioned 1.～the most extremely
Few 2 times,
Above-mentioned steps 1. middle vacuum bagged liquid bio goods are cooled to-5～8 DEG C in advance, are placed in the ice-cold liquid of-5～8 DEG C
In, biological product bag is together forced into 200～300MPa together with ice-cold liquid.
Described step cycle-index 4. is more than or equal to 2 times, but after last pressurization, the temperature of ice-cold liquid is little
In 10 DEG C.
Described stepCycle-index more than or equal to 2 times, change the ice-cold liquid of-5～8 DEG C after pressurization every time
The ice-cold liquid of described-5～8 DEG C takes from the ice-cold liquid outlet of cooling-water machine through super-pressure pump, ice-cold in cooling-water machine
The temperature of liquid is lower than the temperature of vacuum bag 0.5～1 DEG C, because ice-cold liquid is after super-pressure pump, temperature can rise.
Claims (7)
1. the supertension inactivation equipment of pathogen in liquid bio goods, including ultrahigh pressure vessel and connects with ultrahigh pressure vessel
Super-pressure pump, the water inlet end of the outlet ultrahigh pressure vessel of super-pressure pump, it is characterised in that: the inlet communication of super-pressure pump is cold
The outlet of water machine, the water side of the water inlet connection ultrahigh pressure vessel of cooling-water machine.
2. the supertension inactivation equipment of pathogen in liquid bio goods as claimed in claim 1, it is characterised in that: supertension
Pipeline between delivery side of pump and the water inlet end of ultrahigh pressure vessel is provided with check valve, the import of super-pressure pump and cooling-water machine
Pipeline between outlet is provided with check valve.
3. the supertension inactivation equipment of pathogen in liquid bio goods as claimed in claim 1, it is characterised in that: described super
The water inlet end of high-pressure bottle and water side are respectively water inlet port and the water outlet port of three-way valve, the intake-outlet of ultrahigh pressure vessel
Connect with the another port of three-way valve.
4. the supertension inactivation equipment of pathogen in liquid bio goods as claimed in claim 1, it is characterised in that: described super
High-pressure bottle is the cylinder body being provided with column blind hole, and inferior part of the cylinder block accommodates the position of ice-cold liquid and biological product bag and sets collet,
Collet coordinates with cylinder gap, and inferior part of the cylinder block accommodates the position of ice-cold liquid and biological product bag and sets collet, collet with
Cylinder gap sets plunger rod in coordinating column blind hole, sets sealing ring between plunger rod and column blind hole, and cylinder body is fixed on base,
Plunger rod is fixed on the piston rod of the first hydraulic cylinder, and the first hydraulic cylinder is fixed on door type frame, and door type frame is fixed with second
Hydraulic cylinder, the piston rod of the second hydraulic cylinder is fixed with U-shaped piece.
5. the supertension inactivation equipment of pathogen in liquid bio goods as claimed in claim 4, it is characterised in that: the described end
Seat and door type frame relative movement.
6. the supertension inactivation equipment of pathogen in liquid bio goods as claimed in claim 5, it is characterised in that: the described end
Seat is connected with frame by guide rail, and door type frame is fixed in frame, and the piston rod of base and the 3rd hydraulic cylinder is fixing to be connected, and the 3rd
Hydraulic cylinder is fixed in frame.
7. the supertension inactivation equipment of pathogen in liquid bio goods as claimed in claim 5, it is characterised in that: the described end
Seat is fixed in frame, and door type frame is connected with frame by guide rail, and the piston rod of door type frame and the 3rd hydraulic cylinder is fixing to be connected, the
Three hydraulic cylinders are fixed in frame.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620464243.5U CN205814714U (en) | 2016-05-21 | 2016-05-21 | The supertension inactivation equipment of pathogen in liquid bio goods |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620464243.5U CN205814714U (en) | 2016-05-21 | 2016-05-21 | The supertension inactivation equipment of pathogen in liquid bio goods |
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| Publication Number | Publication Date |
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| CN205814714U true CN205814714U (en) | 2016-12-21 |
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| CN201620464243.5U Active CN205814714U (en) | 2016-05-21 | 2016-05-21 | The supertension inactivation equipment of pathogen in liquid bio goods |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107804716A (en) * | 2017-11-03 | 2018-03-16 | 天津华泰森淼生物工程技术股份有限公司 | A kind of Dumpage type extra-high tension unit of automatic opening |
| CN107899029A (en) * | 2017-12-21 | 2018-04-13 | 郑州飞龙医疗设备有限公司 | Fibrous composite super-pressure inactivates nacelle |
| CN108030935A (en) * | 2017-12-21 | 2018-05-15 | 郑州飞龙医疗设备有限公司 | Light-duty integration liquid bio product super-pressure inactivation equipment |
| CN108096590A (en) * | 2017-12-21 | 2018-06-01 | 郑州飞龙医疗设备有限公司 | Light duty liquid biological products super-pressure inactivates equipment |
-
2016
- 2016-05-21 CN CN201620464243.5U patent/CN205814714U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107804716A (en) * | 2017-11-03 | 2018-03-16 | 天津华泰森淼生物工程技术股份有限公司 | A kind of Dumpage type extra-high tension unit of automatic opening |
| CN107804716B (en) * | 2017-11-03 | 2023-10-13 | 天津华泰森淼生物工程技术股份有限公司 | Automatic open toppling type ultrahigh pressure equipment |
| CN107899029A (en) * | 2017-12-21 | 2018-04-13 | 郑州飞龙医疗设备有限公司 | Fibrous composite super-pressure inactivates nacelle |
| CN108030935A (en) * | 2017-12-21 | 2018-05-15 | 郑州飞龙医疗设备有限公司 | Light-duty integration liquid bio product super-pressure inactivation equipment |
| CN108096590A (en) * | 2017-12-21 | 2018-06-01 | 郑州飞龙医疗设备有限公司 | Light duty liquid biological products super-pressure inactivates equipment |
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